AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0136550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136550 MtrunA17_Chr1g0208031 98.603 358 5 0 1 358 1 358 0.0 723
Msa0136550 MtrunA17_Chr5g0411911 52.023 346 166 0 9 354 13 358 3.56e-124 361
Msa0136550 MtrunA17_Chr5g0411801 51.312 343 167 0 9 351 43 385 1.78e-122 358
Msa0136550 MtrunA17_Chr5g0411871 52.000 350 168 0 2 351 19 368 5.45e-122 356
Msa0136550 MtrunA17_Chr5g0411841 52.507 339 161 0 13 351 15 353 9.73e-122 355
Msa0136550 MtrunA17_Chr4g0064291 50.708 353 172 1 1 351 1 353 1.57e-120 352
Msa0136550 MtrunA17_Chr5g0411901 50.729 343 169 0 9 351 12 354 1.73e-120 352
Msa0136550 MtrunA17_Chr5g0411881 50.146 343 171 0 9 351 12 354 5.67e-120 351
Msa0136550 MtrunA17_Chr5g0411821 52.507 339 161 0 13 351 15 353 3.06e-119 350
Msa0136550 MtrunA17_Chr5g0411931 50.729 343 169 0 9 351 11 353 4.79e-119 348
Msa0136550 MtrunA17_Chr8g0367151 52.478 343 163 0 9 351 11 353 1.24e-118 347
Msa0136550 MtrunA17_Chr5g0411811 53.097 339 159 0 13 351 15 353 1.34e-116 342
Msa0136550 MtrunA17_Chr5g0411941 51.297 347 169 0 8 354 10 356 6.41e-114 335
Msa0136550 MtrunA17_Chr1g0157021 49.280 347 175 1 9 354 10 356 4.02e-109 323
Msa0136550 MtrunA17_Chr8g0383721 50.292 342 169 1 13 354 15 355 2.29e-108 321
Msa0136550 MtrunA17_Chr5g0411791 51.863 322 155 0 30 351 6 327 8.44e-105 311
Msa0136550 MtrunA17_Chr2g0279791 44.759 353 193 2 3 354 53 404 1.78e-99 300
Msa0136550 MtrunA17_Chr2g0279801 41.706 211 112 2 144 354 13 212 1.94e-41 144
Msa0136550 MtrunA17_Chr8g0385221 25.269 372 242 10 13 351 56 424 5.26e-24 102
Msa0136550 MtrunA17_Chr5g0411851 50.820 61 30 0 9 69 11 71 2.81e-18 79.7
Msa0136550 MtrunA17_Chr5g0411861 44.068 59 33 0 296 354 2 60 8.19e-14 65.9
Msa0136550 MtrunA17_Chr5g0440521 25.000 340 221 9 35 342 44 381 4.63e-13 69.7
Msa0136550 MtrunA17_Chr3g0125961 23.582 335 226 8 37 342 37 370 3.09e-11 64.3
Msa0136550 MtrunA17_Chr3g0125911 23.881 335 225 8 37 342 37 370 3.09e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136550 AT3G19450.1 76.536 358 84 0 1 358 1 358 0.0 525
Msa0136550 AT4G34230.1 77.311 357 80 1 1 357 1 356 5.34e-178 498
Msa0136550 AT4G34230.2 77.031 357 81 1 1 357 1 356 6.20e-177 495
Msa0136550 AT2G21890.1 49.432 352 177 1 8 358 4 355 2.31e-121 355
Msa0136550 AT4G39330.1 50.877 342 168 0 13 354 15 356 2.41e-120 352
Msa0136550 AT2G21730.1 49.292 353 177 2 8 358 4 356 6.47e-120 351
Msa0136550 AT4G37970.1 48.830 342 175 0 13 354 16 357 3.70e-118 347
Msa0136550 AT4G37990.1 51.009 347 170 0 8 354 6 352 1.54e-113 335
Msa0136550 AT4G37980.1 47.839 347 181 0 8 354 6 352 7.88e-108 320
Msa0136550 AT1G72680.1 43.503 354 199 1 1 354 1 353 1.57e-97 293
Msa0136550 AT4G39330.2 49.481 289 146 0 13 301 15 303 1.36e-96 290
Msa0136550 AT4G37970.4 47.857 280 146 0 13 292 16 295 2.10e-89 271
Msa0136550 AT4G37980.2 48.239 284 146 1 8 290 6 289 1.08e-82 254
Msa0136550 AT4G37970.3 50.000 228 114 0 13 240 16 243 7.48e-75 232
Msa0136550 AT4G37970.2 50.000 228 114 0 13 240 16 243 1.14e-74 232
Msa0136550 AT5G63620.1 26.005 373 238 12 13 351 58 426 5.47e-24 102
Msa0136550 AT5G63620.2 25.737 373 239 12 13 351 58 426 8.80e-24 102
Msa0136550 AT5G63620.3 28.870 239 145 6 13 229 58 293 1.74e-21 94.0
Msa0136550 AT1G32780.1 25.439 342 215 10 37 339 37 377 4.70e-14 73.2
Msa0136550 AT1G22430.2 26.254 339 211 13 37 340 42 376 1.97e-13 71.2
Msa0136550 AT1G22430.1 26.254 339 211 13 37 340 42 376 1.97e-13 71.2
Msa0136550 AT1G64710.2 23.512 336 224 10 37 340 13 347 1.34e-11 65.5
Msa0136550 AT1G64710.1 23.512 336 224 10 37 340 36 370 1.74e-11 65.5
Msa0136550 AT4G22110.2 24.478 335 218 11 37 338 43 375 2.15e-11 65.1
Msa0136550 AT4G22110.1 24.478 335 218 11 37 338 43 375 2.15e-11 65.1

Find 115 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAGTCCACTTTCACATTT+TGG 0.140332 1_3:+68564273 Msa0136550:CDS
GAAACTAAAGCAATAATATT+TGG 0.171337 1_3:-68565628 None:intergenic
CAGAGGAGATGTTGGAGTTT+TGG 0.195897 1_3:+68565048 Msa0136550:CDS
CCCAGGAACCATAGGATAAT+TGG 0.245188 1_3:-68563231 None:intergenic
CATGATTGAAATTGTAACTA+TGG 0.253443 1_3:+68565091 Msa0136550:CDS
GCTTACCTAATATTTGAAAC+TGG 0.254010 1_3:+68565565 Msa0136550:three_prime_UTR
ATGTCCCAGTTTCAAATATT+AGG 0.285207 1_3:-68565570 None:intergenic
TCCAATTATCCTATGGTTCC+TGG 0.314697 1_3:+68563230 Msa0136550:CDS
AAGCAATAATATTTGGCATT+TGG 0.317520 1_3:-68565621 None:intergenic
AGTCGTGTTTGCTTTGCTTT+TGG 0.333364 1_3:+68565487 Msa0136550:three_prime_UTR
CTTTCATTGCTCAAAATTGA+TGG 0.339894 1_3:+68564552 Msa0136550:CDS
TGGGCTAAGAGGTGGAATAT+TGG 0.354895 1_3:+68564308 Msa0136550:CDS
TCAATTACTGGAAGCTTTGT+TGG 0.375046 1_3:+68565011 Msa0136550:CDS
GGGCTAAGAGGTGGAATATT+GGG 0.377765 1_3:+68564309 Msa0136550:CDS
CATAGTTACAATTTCAATCA+TGG 0.394791 1_3:-68565090 None:intergenic
GGCTCAAGAGGGTGACCAAC+AGG 0.414159 1_3:-68564525 None:intergenic
TCTTCAATTTGTTACACCTA+TGG 0.415794 1_3:+68564608 Msa0136550:CDS
AAAGAGGCACTTGAGGATCT+TGG 0.422297 1_3:+68564420 Msa0136550:CDS
TGATGGGAAATTGATCTTGA+TGG 0.430428 1_3:+68564569 Msa0136550:CDS
AACAATGGTGCAACTTGCTC+TGG 0.430947 1_3:-68564231 None:intergenic
TGCTCTGGTGCCAATCCCTC+TGG 0.434175 1_3:-68564216 None:intergenic
CAATTACTGGAAGCTTTGTT+GGG 0.441579 1_3:+68565012 Msa0136550:CDS
GTGAAAGTAGCAAAGGCATT+AGG 0.442216 1_3:+68564357 Msa0136550:CDS
TTGTTGTGTTTGTATAATTG+AGG 0.442514 1_3:+68565269 Msa0136550:three_prime_UTR
CATGATTGCTTAGGGAATGA+AGG 0.442522 1_3:+68565665 Msa0136550:three_prime_UTR
AGAAGAATGATGTGAGGTAT+AGG 0.457166 1_3:+68565144 Msa0136550:CDS
CCGTCCTATGTTATTCCAAC+GGG 0.464767 1_3:+68565707 Msa0136550:three_prime_UTR
GTAGGGAGGAAGTCAATTAC+TGG 0.465862 1_3:+68564999 Msa0136550:intron
TGGCATTTGGCATACGTGTA+TGG 0.466430 1_3:-68565608 None:intergenic
TTGTTAGCTCAGATACTGTT+GGG 0.467194 1_3:+68564457 Msa0136550:CDS
CTTGTTAGCTCAGATACTGT+TGG 0.468029 1_3:+68564456 Msa0136550:CDS
GTTTCAAATATTAGGTAAGC+AGG 0.470896 1_3:-68565562 None:intergenic
AGTGCAAACGTGAGTGGGAT+TGG 0.475366 1_3:-68562827 None:intergenic
TGGCATGTCCAATTATCCTA+TGG 0.476856 1_3:+68563223 Msa0136550:CDS
ACGTGAGTGGGATTGGTTAG+TGG 0.477400 1_3:-68562820 None:intergenic
GAAGGAGACAGAGGAGATGT+TGG 0.486422 1_3:+68565040 Msa0136550:CDS
TACAATGATGTTTACACTGA+CGG 0.486763 1_3:+68563571 Msa0136550:CDS
CTCCATACACATACACTCTC+AGG 0.487023 1_3:+68562979 Msa0136550:CDS
TTTCATTGCTCAAAATTGAT+GGG 0.487627 1_3:+68564553 Msa0136550:CDS
AATACTGCAACAAGAAGATC+TGG 0.488627 1_3:+68563545 Msa0136550:CDS
AAGTAAATTTGAGGAGTGAA+AGG 0.492257 1_3:+68565187 Msa0136550:exon
TGTGAAAATACCAGAGGGAT+TGG 0.495073 1_3:+68564206 Msa0136550:CDS
TTGGCTGCAAAAGACCCTTC+CGG 0.496088 1_3:+68562948 Msa0136550:CDS
CACCTGAGAGTGTATGTGTA+TGG 0.504281 1_3:-68562981 None:intergenic
TTCAGCCCAAAATGTGAAAG+TGG 0.506368 1_3:-68564279 None:intergenic
AGAGGTGGAATATTGGGTCT+TGG 0.506833 1_3:+68564315 Msa0136550:CDS
GAAAACAGTGCAAACGTGAG+TGG 0.508827 1_3:-68562833 None:intergenic
GTTGCACCATTGTTGTGTGC+AGG 0.511420 1_3:+68564240 Msa0136550:CDS
CTTGGTGGAGTAGGACACAT+GGG 0.512497 1_3:+68564333 Msa0136550:CDS
TGTGTATGGAGTGAGAATTC+CGG 0.512818 1_3:-68562967 None:intergenic
CTTACCTAATATTTGAAACT+GGG 0.515181 1_3:+68565566 Msa0136550:three_prime_UTR
GGTGGAATATTGGGTCTTGG+TGG 0.517471 1_3:+68564318 Msa0136550:CDS
AAAATATTTGATAAATCACT+TGG 0.517804 1_3:+68565789 Msa0136550:three_prime_UTR
CTGAAAACACCTGGGCTAAG+AGG 0.518039 1_3:+68564297 Msa0136550:CDS
CCAATTATCCTATGGTTCCT+GGG 0.521261 1_3:+68563231 Msa0136550:CDS
CGTGAGTGGGATTGGTTAGT+GGG 0.524978 1_3:-68562819 None:intergenic
GTGGGAGTTGGTCTTCTCGT+TGG 0.525528 1_3:+68563481 Msa0136550:CDS
GTTCAAAGTGGGAGAAATTG+TGG 0.526442 1_3:+68563462 Msa0136550:CDS
GTGGGAGAAATTGTGGGAGT+TGG 0.528018 1_3:+68563469 Msa0136550:CDS
AGAAAGAACAACAGTAGGAT+TGG 0.528316 1_3:+68562929 Msa0136550:CDS
GAAGAAGAAAGAGGCACTTG+AGG 0.529985 1_3:+68564413 Msa0136550:CDS
TGTCAAAGGAAGTAAATTTG+AGG 0.531733 1_3:+68565178 Msa0136550:CDS
AGTAAATTTGAGGAGTGAAA+GGG 0.536045 1_3:+68565188 Msa0136550:exon
GGTTGGTGAGGTACTAGAGG+TGG 0.538320 1_3:+68563423 Msa0136550:CDS
CATACATACCCAGGAACCAT+AGG 0.538858 1_3:-68563239 None:intergenic
GATGGGAAATTGATCTTGAT+GGG 0.539815 1_3:+68564570 Msa0136550:CDS
GTTACACCTATGGTCATGCT+AGG 0.542756 1_3:+68564618 Msa0136550:CDS
TTCAAAGTGGGAGAAATTGT+GGG 0.545521 1_3:+68563463 Msa0136550:CDS
ATCATTGACACTGTTCCTGT+TGG 0.558930 1_3:+68564510 Msa0136550:CDS
ATATTGGGTCTTGGTGGAGT+AGG 0.559461 1_3:+68564324 Msa0136550:CDS
TTCTGATAAGAAGAAGAAAG+AGG 0.560483 1_3:+68564404 Msa0136550:CDS
AGGTGGGTTCAAATGTTACA+AGG 0.565769 1_3:+68563440 Msa0136550:CDS
GTTGGTGAGGTACTAGAGGT+GGG 0.567462 1_3:+68563424 Msa0136550:CDS
AGGTTCGTTGTGGATGTCAA+AGG 0.573106 1_3:+68565164 Msa0136550:CDS
AGGTAAAGATTTGTCAAAGT+AGG 0.575343 1_3:-68565542 None:intergenic
AAGTCCCGTTGGAATAACAT+AGG 0.576858 1_3:-68565711 None:intergenic
TAGGGAATGAAGGTATTGCA+TGG 0.578144 1_3:+68565675 Msa0136550:three_prime_UTR
TACATCAAAATTCATTACTG+TGG 0.582501 1_3:+68563161 Msa0136550:CDS
TCCGTCCTATGTTATTCCAA+CGG 0.584475 1_3:+68565706 Msa0136550:three_prime_UTR
AGGGAATGAAGGTATTGCAT+GGG 0.585204 1_3:+68565676 Msa0136550:three_prime_UTR
CCCGTTGGAATAACATAGGA+CGG 0.587997 1_3:-68565707 None:intergenic
TTGGTGGAGTAGGACACATG+GGG 0.596788 1_3:+68564334 Msa0136550:CDS
AAATGTTACAAGGTTCAAAG+TGG 0.597798 1_3:+68563450 Msa0136550:CDS
AAAACAGTGCAAACGTGAGT+GGG 0.598960 1_3:-68562832 None:intergenic
AATGTTACAAGGTTCAAAGT+GGG 0.599126 1_3:+68563451 Msa0136550:CDS
ACTCTCGTTCTCAAAAGAGT+TGG 0.599535 1_3:-68565467 None:intergenic
TAGGTGTAACAAATTGAAGA+GGG 0.600517 1_3:-68564605 None:intergenic
ATAGGTGTAACAAATTGAAG+AGG 0.601584 1_3:-68564606 None:intergenic
AATATTCCACCTCTTAGCCC+AGG 0.603820 1_3:-68564306 None:intergenic
AAAACACCTGGGCTAAGAGG+TGG 0.604672 1_3:+68564300 Msa0136550:CDS
GTGAGTATAATAAGTCCCGT+TGG 0.605685 1_3:-68565722 None:intergenic
AATGAAAGATAAGGCTCAAG+AGG 0.608696 1_3:-68564537 None:intergenic
TCTTGGTGGAGTAGGACACA+TGG 0.610428 1_3:+68564332 Msa0136550:CDS
GCTGCAGAAAGAACAACAGT+AGG 0.613158 1_3:+68562924 Msa0136550:CDS
TGTGAGGTATAGGTTCGTTG+TGG 0.619087 1_3:+68565154 Msa0136550:CDS
AAGCTTTGTTGGGAGCGTGA+AGG 0.622128 1_3:+68565022 Msa0136550:CDS
TTTGTTGTGAAAATACCAGA+GGG 0.624457 1_3:+68564201 Msa0136550:CDS
CAGGCATGAAGTGGTTGGTG+AGG 0.629500 1_3:+68563411 Msa0136550:intron
CACTGACGGAAAGATCACTC+AGG 0.632595 1_3:+68563585 Msa0136550:CDS
TTAAAACTATGCAGAAACAC+AGG 0.640578 1_3:+68563125 Msa0136550:intron
ACTATGCAGAAACACAGGAC+CGG 0.643408 1_3:+68563130 Msa0136550:intron
ATACAATATCATGAAGAGTG+TGG 0.644208 1_3:+68565229 Msa0136550:three_prime_UTR
GTCACACCTGCACACAACAA+TGG 0.650120 1_3:-68564246 None:intergenic
TGGGAGCGTGAAGGAGACAG+AGG 0.652158 1_3:+68565031 Msa0136550:CDS
ATGAAAGATAAGGCTCAAGA+GGG 0.658619 1_3:-68564536 None:intergenic
GATTGGAGAAGAATGATGTG+AGG 0.663743 1_3:+68565138 Msa0136550:CDS
GTTTGTTGTGAAAATACCAG+AGG 0.664399 1_3:+68564200 Msa0136550:CDS
AGTGGTTGGTGAGGTACTAG+AGG 0.677629 1_3:+68563420 Msa0136550:CDS
ACTGACGGAAAGATCACTCA+GGG 0.682017 1_3:+68563586 Msa0136550:CDS
TATGGAGTGAGAATTCCGGA+AGG 0.698628 1_3:-68562963 None:intergenic
ATGGAGTGAGAATTCCGGAA+GGG 0.705897 1_3:-68562962 None:intergenic
AAGAGCTTATCACAGTGACA+TGG 0.707377 1_3:-68564383 None:intergenic
AGGTGTAACAAATTGAAGAG+GGG 0.708980 1_3:-68564604 None:intergenic
GACGGAAAGATCACTCAGGG+TGG 0.713421 1_3:+68563589 Msa0136550:CDS
TGGTGGAGTAGGACACATGG+GGG 0.751756 1_3:+68564335 Msa0136550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAGTAATTAATTTAGTT+GGG - chr1_3:68564083-68564102 None:intergenic 10.0%
!! ATAAAAGTAATTAATTTAGT+TGG - chr1_3:68564084-68564103 None:intergenic 10.0%
!! TAAAAATTACTAACAAATTT+AGG - chr1_3:68564758-68564777 None:intergenic 10.0%
!! ATATAATTAGAAAAATTAGA+AGG + chr1_3:68565410-68565429 Msa0136550:three_prime_UTR 10.0%
!!! ATCATCAATGTTTTTTAAAT+GGG - chr1_3:68563750-68563769 None:intergenic 15.0%
!!! AATCATCAATGTTTTTTAAA+TGG - chr1_3:68563751-68563770 None:intergenic 15.0%
!!! ATGTTATGAAAAATGTTTTA+TGG + chr1_3:68563901-68563920 Msa0136550:intron 15.0%
!!! ATTAATTACTTTTATGAATC+TGG + chr1_3:68564089-68564108 Msa0136550:intron 15.0%
!!! CTTTTAAATAACTTGTAATT+AGG + chr1_3:68564806-68564825 Msa0136550:intron 15.0%
!!! TTATTTAAAAATCTAGTTCA+AGG + chr1_3:68564856-68564875 Msa0136550:intron 15.0%
!! AAAATATTTGATAAATCACT+TGG + chr1_3:68565789-68565808 Msa0136550:three_prime_UTR 15.0%
!! ATCTATCAAATTATTATGTC+CGG + chr1_3:68563287-68563306 Msa0136550:intron 20.0%
!!! TATGATTGTTTTGAAAAAAC+AGG + chr1_3:68563392-68563411 Msa0136550:intron 20.0%
!! AATTTAGATATGTTCAATTG+TGG + chr1_3:68563997-68564016 Msa0136550:intron 20.0%
!! TTCACGGTAAAAAAAAAATA+TGG - chr1_3:68564044-68564063 None:intergenic 20.0%
!!! TTTTAAACTGATTATGTGTT+TGG + chr1_3:68564162-68564181 Msa0136550:intron 20.0%
!! TTTAAACTGATTATGTGTTT+GGG + chr1_3:68564163-68564182 Msa0136550:intron 20.0%
!!! TTTTAGCTTAGTTATTGAAT+AGG + chr1_3:68564673-68564692 Msa0136550:intron 20.0%
!! TTAAAATCTTCAATAGTCAA+GGG - chr1_3:68564700-68564719 None:intergenic 20.0%
!!! TTTAAAATCTTCAATAGTCA+AGG - chr1_3:68564701-68564720 None:intergenic 20.0%
!!! CTTTAAGTTATTGAACAATT+TGG - chr1_3:68564898-68564917 None:intergenic 20.0%
!! TTAGAAGATATCATTACAAT+TGG - chr1_3:68564960-68564979 None:intergenic 20.0%
!! GAAACTAAAGCAATAATATT+TGG - chr1_3:68565631-68565650 None:intergenic 20.0%
!!! ATTTTGATGTAAACATCATC+CGG - chr1_3:68563152-68563171 None:intergenic 25.0%
! TACATCAAAATTCATTACTG+TGG + chr1_3:68563161-68563180 Msa0136550:CDS 25.0%
! CCTTAAAATTAATCGAAACA+CGG - chr1_3:68563329-68563348 None:intergenic 25.0%
!!! TAATTACTTTTGTTCAACGA+CGG - chr1_3:68563629-68563648 None:intergenic 25.0%
! AAAACGTGTGGAATTTAAAT+TGG - chr1_3:68563711-68563730 None:intergenic 25.0%
!! TTTTTCATAACATAAGCGTT+TGG - chr1_3:68563894-68563913 None:intergenic 25.0%
! TAACAGGTGTGAAAAAAATT+GGG - chr1_3:68563930-68563949 None:intergenic 25.0%
! TTAACAGGTGTGAAAAAAAT+TGG - chr1_3:68563931-68563950 None:intergenic 25.0%
! TTCAATTGTGGTTAATTATG+TGG + chr1_3:68564009-68564028 Msa0136550:intron 25.0%
!! TACACAGTTAATTTAGTTCA+CGG - chr1_3:68564060-68564079 None:intergenic 25.0%
!! TTTTATGAATCTGGTGTAAA+AGG + chr1_3:68564098-68564117 Msa0136550:intron 25.0%
! TTAAACTGATTATGTGTTTG+GGG + chr1_3:68564164-68564183 Msa0136550:intron 25.0%
!! ATTTTGAGCAATGAAAGATA+AGG - chr1_3:68564549-68564568 None:intergenic 25.0%
! TTTCATTGCTCAAAATTGAT+GGG + chr1_3:68564553-68564572 Msa0136550:CDS 25.0%
! TGTAATTAGGTCTTGAATTT+AGG + chr1_3:68564819-68564838 Msa0136550:intron 25.0%
! GTAATTAGGTCTTGAATTTA+GGG + chr1_3:68564820-68564839 Msa0136550:intron 25.0%
! CATAGTTACAATTTCAATCA+TGG - chr1_3:68565093-68565112 None:intergenic 25.0%
! CATGATTGAAATTGTAACTA+TGG + chr1_3:68565091-68565110 Msa0136550:CDS 25.0%
!!! TAACAAAGCTTTTGAAAGAT+TGG + chr1_3:68565121-68565140 Msa0136550:CDS 25.0%
! TTGTTGTGTTTGTATAATTG+AGG + chr1_3:68565269-68565288 Msa0136550:three_prime_UTR 25.0%
! TAGAAGGAAAGAAAAACAAA+AGG + chr1_3:68565426-68565445 Msa0136550:three_prime_UTR 25.0%
! AGAAAAACAAAAGGATATGT+TGG + chr1_3:68565435-68565454 Msa0136550:three_prime_UTR 25.0%
! GAAAAACAAAAGGATATGTT+GGG + chr1_3:68565436-68565455 Msa0136550:three_prime_UTR 25.0%
!! CTTACCTAATATTTGAAACT+GGG + chr1_3:68565566-68565585 Msa0136550:three_prime_UTR 25.0%
! AAGCAATAATATTTGGCATT+TGG - chr1_3:68565624-68565643 None:intergenic 25.0%
!!! TTTGTTTTCATGATTGCTTA+GGG + chr1_3:68565657-68565676 Msa0136550:three_prime_UTR 25.0%
TTAAAACTATGCAGAAACAC+AGG + chr1_3:68563125-68563144 Msa0136550:intron 30.0%
CACCAAATCAAAAATGATCT+TGG + chr1_3:68563203-68563222 Msa0136550:CDS 30.0%
!! TGCCAAGATCATTTTTGATT+TGG - chr1_3:68563208-68563227 None:intergenic 30.0%
! CCGTGTTTCGATTAATTTTA+AGG + chr1_3:68563326-68563345 Msa0136550:intron 30.0%
AAATGTTACAAGGTTCAAAG+TGG + chr1_3:68563450-68563469 Msa0136550:CDS 30.0%
AATGTTACAAGGTTCAAAGT+GGG + chr1_3:68563451-68563470 Msa0136550:CDS 30.0%
TACAATGATGTTTACACTGA+CGG + chr1_3:68563571-68563590 Msa0136550:CDS 30.0%
CAAGTTTGTATGTGATTAAC+AGG - chr1_3:68563946-68563965 None:intergenic 30.0%
! TTTGTTGTGAAAATACCAGA+GGG + chr1_3:68564201-68564220 Msa0136550:CDS 30.0%
TTCTGATAAGAAGAAGAAAG+AGG + chr1_3:68564404-68564423 Msa0136550:CDS 30.0%
CTTTCATTGCTCAAAATTGA+TGG + chr1_3:68564552-68564571 Msa0136550:CDS 30.0%
TAGGTGTAACAAATTGAAGA+GGG - chr1_3:68564608-68564627 None:intergenic 30.0%
ATAGGTGTAACAAATTGAAG+AGG - chr1_3:68564609-68564628 None:intergenic 30.0%
!! TCTTCAATTTGTTACACCTA+TGG + chr1_3:68564608-68564627 Msa0136550:CDS 30.0%
ATCATTGATGAGAAAGAAGA+AGG - chr1_3:68564653-68564672 None:intergenic 30.0%
TGTCAAAGGAAGTAAATTTG+AGG + chr1_3:68565178-68565197 Msa0136550:CDS 30.0%
AAGTAAATTTGAGGAGTGAA+AGG + chr1_3:68565187-68565206 Msa0136550:exon 30.0%
AGTAAATTTGAGGAGTGAAA+GGG + chr1_3:68565188-68565207 Msa0136550:exon 30.0%
ATACAATATCATGAAGAGTG+TGG + chr1_3:68565229-68565248 Msa0136550:three_prime_UTR 30.0%
! TTTCTCTCTTTTCTATGCTA+TGG + chr1_3:68565294-68565313 Msa0136550:three_prime_UTR 30.0%
AGGTAAAGATTTGTCAAAGT+AGG - chr1_3:68565545-68565564 None:intergenic 30.0%
GTTTCAAATATTAGGTAAGC+AGG - chr1_3:68565565-68565584 None:intergenic 30.0%
! GCTTACCTAATATTTGAAAC+TGG + chr1_3:68565565-68565584 Msa0136550:three_prime_UTR 30.0%
ATGTCCCAGTTTCAAATATT+AGG - chr1_3:68565573-68565592 None:intergenic 30.0%
!!! CTTTGTTTTCATGATTGCTT+AGG + chr1_3:68565656-68565675 Msa0136550:three_prime_UTR 30.0%
ACCAGAGAACACAAAAACAT+GGG + chr1_3:68562891-68562910 Msa0136550:exon 35.0%
AGAAAGAACAACAGTAGGAT+TGG + chr1_3:68562929-68562948 Msa0136550:CDS 35.0%
AGAAGTTAACATACATACCC+AGG - chr1_3:68563251-68563270 None:intergenic 35.0%
TTATTATGTCCGGTGTTTGT+TGG + chr1_3:68563297-68563316 Msa0136550:intron 35.0%
TTGAAAAAACAGGCATGAAG+TGG + chr1_3:68563402-68563421 Msa0136550:intron 35.0%
TTCAAAGTGGGAGAAATTGT+GGG + chr1_3:68563463-68563482 Msa0136550:CDS 35.0%
AATACTGCAACAAGAAGATC+TGG + chr1_3:68563545-68563564 Msa0136550:CDS 35.0%
GTGTGGAATTTAAATTGGCA+CGG - chr1_3:68563706-68563725 None:intergenic 35.0%
TCAGAAATGCATAAAACGTG+TGG - chr1_3:68563723-68563742 None:intergenic 35.0%
!! GTTTGTTGTGAAAATACCAG+AGG + chr1_3:68564200-68564219 Msa0136550:CDS 35.0%
! TACAGTCCACTTTCACATTT+TGG + chr1_3:68564273-68564292 Msa0136550:CDS 35.0%
! ACAGTCCACTTTCACATTTT+GGG + chr1_3:68564274-68564293 Msa0136550:CDS 35.0%
TTGTTAGCTCAGATACTGTT+GGG + chr1_3:68564457-68564476 Msa0136550:CDS 35.0%
ATGAAAGATAAGGCTCAAGA+GGG - chr1_3:68564539-68564558 None:intergenic 35.0%
AATGAAAGATAAGGCTCAAG+AGG - chr1_3:68564540-68564559 None:intergenic 35.0%
TGATGGGAAATTGATCTTGA+TGG + chr1_3:68564569-68564588 Msa0136550:CDS 35.0%
GATGGGAAATTGATCTTGAT+GGG + chr1_3:68564570-68564589 Msa0136550:CDS 35.0%
AGGTGTAACAAATTGAAGAG+GGG - chr1_3:68564607-68564626 None:intergenic 35.0%
TTGATGAGAAAGAAGAAGGT+GGG - chr1_3:68564649-68564668 None:intergenic 35.0%
ATTGATGAGAAAGAAGAAGG+TGG - chr1_3:68564650-68564669 None:intergenic 35.0%
TATTGATGTTGTGTTGCGTA+GGG + chr1_3:68564982-68565001 Msa0136550:intron 35.0%
! TCAATTACTGGAAGCTTTGT+TGG + chr1_3:68565011-68565030 Msa0136550:CDS 35.0%
!! CAATTACTGGAAGCTTTGTT+GGG + chr1_3:68565012-68565031 Msa0136550:CDS 35.0%
!!! TTGGAGTTTTGGAAAGAGAA+GGG + chr1_3:68565059-68565078 Msa0136550:CDS 35.0%
AGAAGAATGATGTGAGGTAT+AGG + chr1_3:68565144-68565163 Msa0136550:CDS 35.0%
CTGTGCGTAAAAAACTAAAG+AGG - chr1_3:68565830-68565849 None:intergenic 35.0%
!!! CTCTTTAGTTTTTTACGCAC+AGG + chr1_3:68565828-68565847 Msa0136550:three_prime_UTR 35.0%
AAAACAGTGCAAACGTGAGT+GGG - chr1_3:68562835-68562854 None:intergenic 40.0%
GACCAGAGAACACAAAAACA+TGG + chr1_3:68562890-68562909 Msa0136550:exon 40.0%
!! ACCCATGTTTTTGTGTTCTC+TGG - chr1_3:68562895-68562914 None:intergenic 40.0%
TGTGTATGGAGTGAGAATTC+CGG - chr1_3:68562970-68562989 None:intergenic 40.0%
TGGCATGTCCAATTATCCTA+TGG + chr1_3:68563223-68563242 Msa0136550:CDS 40.0%
TCCAATTATCCTATGGTTCC+TGG + chr1_3:68563230-68563249 Msa0136550:CDS 40.0%
CCAATTATCCTATGGTTCCT+GGG + chr1_3:68563231-68563250 Msa0136550:CDS 40.0%
AAAAACAGGCATGAAGTGGT+TGG + chr1_3:68563406-68563425 Msa0136550:intron 40.0%
! AGGTGGGTTCAAATGTTACA+AGG + chr1_3:68563440-68563459 Msa0136550:CDS 40.0%
GTTCAAAGTGGGAGAAATTG+TGG + chr1_3:68563462-68563481 Msa0136550:CDS 40.0%
!! TTACTTTTGTTCAACGACGG+TGG - chr1_3:68563626-68563645 None:intergenic 40.0%
TGTTTGGGGATATTACTGTC+AGG + chr1_3:68564178-68564197 Msa0136550:intron 40.0%
TGTGAAAATACCAGAGGGAT+TGG + chr1_3:68564206-68564225 Msa0136550:CDS 40.0%
TTCAGCCCAAAATGTGAAAG+TGG - chr1_3:68564282-68564301 None:intergenic 40.0%
! ATTTTGGGCTGAAAACACCT+GGG + chr1_3:68564289-68564308 Msa0136550:CDS 40.0%
!! GTGAAAGTAGCAAAGGCATT+AGG + chr1_3:68564357-68564376 Msa0136550:CDS 40.0%
AAGAGCTTATCACAGTGACA+TGG - chr1_3:68564386-68564405 None:intergenic 40.0%
CTTGTTAGCTCAGATACTGT+TGG + chr1_3:68564456-68564475 Msa0136550:CDS 40.0%
ATCATTGACACTGTTCCTGT+TGG + chr1_3:68564510-68564529 Msa0136550:CDS 40.0%
! GTTTTACCTAGCATGACCAT+AGG - chr1_3:68564627-68564646 None:intergenic 40.0%
CTATTGATGTTGTGTTGCGT+AGG + chr1_3:68564981-68565000 Msa0136550:intron 40.0%
!!! GTTGGAGTTTTGGAAAGAGA+AGG + chr1_3:68565058-68565077 Msa0136550:CDS 40.0%
GATTGGAGAAGAATGATGTG+AGG + chr1_3:68565138-68565157 Msa0136550:CDS 40.0%
ACTCTCGTTCTCAAAAGAGT+TGG - chr1_3:68565470-68565489 None:intergenic 40.0%
! AGTCGTGTTTGCTTTGCTTT+TGG + chr1_3:68565487-68565506 Msa0136550:three_prime_UTR 40.0%
!! TTTGCTTTTGGTCGTGTTGA+TGG + chr1_3:68565499-68565518 Msa0136550:three_prime_UTR 40.0%
CATGATTGCTTAGGGAATGA+AGG + chr1_3:68565665-68565684 Msa0136550:three_prime_UTR 40.0%
!! TAGGGAATGAAGGTATTGCA+TGG + chr1_3:68565675-68565694 Msa0136550:three_prime_UTR 40.0%
!! AGGGAATGAAGGTATTGCAT+GGG + chr1_3:68565676-68565695 Msa0136550:three_prime_UTR 40.0%
TCCGTCCTATGTTATTCCAA+CGG + chr1_3:68565706-68565725 Msa0136550:three_prime_UTR 40.0%
AAGTCCCGTTGGAATAACAT+AGG - chr1_3:68565714-68565733 None:intergenic 40.0%
GTGAGTATAATAAGTCCCGT+TGG - chr1_3:68565725-68565744 None:intergenic 40.0%
GAAAACAGTGCAAACGTGAG+TGG - chr1_3:68562836-68562855 None:intergenic 45.0%
GCTGCAGAAAGAACAACAGT+AGG + chr1_3:68562924-68562943 Msa0136550:CDS 45.0%
ATGGAGTGAGAATTCCGGAA+GGG - chr1_3:68562965-68562984 None:intergenic 45.0%
TATGGAGTGAGAATTCCGGA+AGG - chr1_3:68562966-68562985 None:intergenic 45.0%
CTCCATACACATACACTCTC+AGG + chr1_3:68562979-68562998 Msa0136550:CDS 45.0%
! CACCTGAGAGTGTATGTGTA+TGG - chr1_3:68562984-68563003 None:intergenic 45.0%
ACTATGCAGAAACACAGGAC+CGG + chr1_3:68563130-68563149 Msa0136550:intron 45.0%
CCCAGGAACCATAGGATAAT+TGG - chr1_3:68563234-68563253 None:intergenic 45.0%
CATACATACCCAGGAACCAT+AGG - chr1_3:68563242-68563261 None:intergenic 45.0%
CGGACATATCCAACAAACAC+CGG - chr1_3:68563309-68563328 None:intergenic 45.0%
ACTGACGGAAAGATCACTCA+GGG + chr1_3:68563586-68563605 Msa0136550:CDS 45.0%
! AACAATGGTGCAACTTGCTC+TGG - chr1_3:68564234-68564253 None:intergenic 45.0%
! CATTTTGGGCTGAAAACACC+TGG + chr1_3:68564288-68564307 Msa0136550:CDS 45.0%
AATATTCCACCTCTTAGCCC+AGG - chr1_3:68564309-68564328 None:intergenic 45.0%
TGGGCTAAGAGGTGGAATAT+TGG + chr1_3:68564308-68564327 Msa0136550:CDS 45.0%
GGGCTAAGAGGTGGAATATT+GGG + chr1_3:68564309-68564328 Msa0136550:CDS 45.0%
AGAGGTGGAATATTGGGTCT+TGG + chr1_3:68564315-68564334 Msa0136550:CDS 45.0%
ATATTGGGTCTTGGTGGAGT+AGG + chr1_3:68564324-68564343 Msa0136550:CDS 45.0%
!! GAAGAAGAAAGAGGCACTTG+AGG + chr1_3:68564413-68564432 Msa0136550:CDS 45.0%
!! AAAGAGGCACTTGAGGATCT+TGG + chr1_3:68564420-68564439 Msa0136550:CDS 45.0%
GTTACACCTATGGTCATGCT+AGG + chr1_3:68564618-68564637 Msa0136550:CDS 45.0%
GTAGGGAGGAAGTCAATTAC+TGG + chr1_3:68564999-68565018 Msa0136550:intron 45.0%
! CAGAGGAGATGTTGGAGTTT+TGG + chr1_3:68565048-68565067 Msa0136550:CDS 45.0%
TGTGAGGTATAGGTTCGTTG+TGG + chr1_3:68565154-68565173 Msa0136550:CDS 45.0%
AGGTTCGTTGTGGATGTCAA+AGG + chr1_3:68565164-68565183 Msa0136550:CDS 45.0%
TGGCATTTGGCATACGTGTA+TGG - chr1_3:68565611-68565630 None:intergenic 45.0%
CCCGTTGGAATAACATAGGA+CGG - chr1_3:68565710-68565729 None:intergenic 45.0%
CCGTCCTATGTTATTCCAAC+GGG + chr1_3:68565707-68565726 Msa0136550:three_prime_UTR 45.0%
!!! TTAAACTTTTTTTTTTAATT+TGG + chr1_3:68564775-68564794 Msa0136550:intron 5.0%
!!! TTTTTAAATAATTTATGTTT+AGG - chr1_3:68564847-68564866 None:intergenic 5.0%
!! CGTGAGTGGGATTGGTTAGT+GGG - chr1_3:68562822-68562841 None:intergenic 50.0%
!! ACGTGAGTGGGATTGGTTAG+TGG - chr1_3:68562823-68562842 None:intergenic 50.0%
AGTGCAAACGTGAGTGGGAT+TGG - chr1_3:68562830-68562849 None:intergenic 50.0%
TTGGCTGCAAAAGACCCTTC+CGG + chr1_3:68562948-68562967 Msa0136550:CDS 50.0%
!! AGTGGTTGGTGAGGTACTAG+AGG + chr1_3:68563420-68563439 Msa0136550:CDS 50.0%
! GTTGGTGAGGTACTAGAGGT+GGG + chr1_3:68563424-68563443 Msa0136550:CDS 50.0%
GTGGGAGAAATTGTGGGAGT+TGG + chr1_3:68563469-68563488 Msa0136550:CDS 50.0%
CACTGACGGAAAGATCACTC+AGG + chr1_3:68563585-68563604 Msa0136550:CDS 50.0%
GTTGCACCATTGTTGTGTGC+AGG + chr1_3:68564240-68564259 Msa0136550:CDS 50.0%
GTCACACCTGCACACAACAA+TGG - chr1_3:68564249-68564268 None:intergenic 50.0%
CTGAAAACACCTGGGCTAAG+AGG + chr1_3:68564297-68564316 Msa0136550:CDS 50.0%
AAAACACCTGGGCTAAGAGG+TGG + chr1_3:68564300-68564319 Msa0136550:CDS 50.0%
GGTGGAATATTGGGTCTTGG+TGG + chr1_3:68564318-68564337 Msa0136550:CDS 50.0%
TCTTGGTGGAGTAGGACACA+TGG + chr1_3:68564332-68564351 Msa0136550:CDS 50.0%
CTTGGTGGAGTAGGACACAT+GGG + chr1_3:68564333-68564352 Msa0136550:CDS 50.0%
TTGGTGGAGTAGGACACATG+GGG + chr1_3:68564334-68564353 Msa0136550:CDS 50.0%
CATGGGGGTGAAAGTAGCAA+AGG + chr1_3:68564350-68564369 Msa0136550:CDS 50.0%
TGATGTTGTGTTGCGTAGGG+AGG + chr1_3:68564985-68565004 Msa0136550:intron 50.0%
!! AAGCTTTGTTGGGAGCGTGA+AGG + chr1_3:68565022-68565041 Msa0136550:CDS 50.0%
GAAGGAGACAGAGGAGATGT+TGG + chr1_3:68565040-68565059 Msa0136550:CDS 50.0%
! CAGGCATGAAGTGGTTGGTG+AGG + chr1_3:68563411-68563430 Msa0136550:intron 55.0%
!! GGTTGGTGAGGTACTAGAGG+TGG + chr1_3:68563423-68563442 Msa0136550:CDS 55.0%
!! GTGGGAGTTGGTCTTCTCGT+TGG + chr1_3:68563481-68563500 Msa0136550:CDS 55.0%
GACGGAAAGATCACTCAGGG+TGG + chr1_3:68563589-68563608 Msa0136550:CDS 55.0%
TGGTGGAGTAGGACACATGG+GGG + chr1_3:68564335-68564354 Msa0136550:CDS 55.0%
! TGCTCTGGTGCCAATCCCTC+TGG - chr1_3:68564219-68564238 None:intergenic 60.0%
GGCTCAAGAGGGTGACCAAC+AGG - chr1_3:68564528-68564547 None:intergenic 60.0%
! TGGGAGCGTGAAGGAGACAG+AGG + chr1_3:68565031-68565050 Msa0136550:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 68562819 68565902 68562819 ID=Msa0136550;Name=Msa0136550
chr1_3 mRNA 68562819 68565902 68562819 ID=Msa0136550-mRNA-1;Parent=Msa0136550;Name=Msa0136550-mRNA-1;_AED=0.07;_eAED=0.07;_QI=90|1|1|1|1|1|5|697|358
chr1_3 exon 68562819 68563000 68562819 ID=Msa0136550-mRNA-1:exon:23646;Parent=Msa0136550-mRNA-1
chr1_3 exon 68563139 68563252 68563139 ID=Msa0136550-mRNA-1:exon:23647;Parent=Msa0136550-mRNA-1
chr1_3 exon 68563414 68563641 68563414 ID=Msa0136550-mRNA-1:exon:23648;Parent=Msa0136550-mRNA-1
chr1_3 exon 68564200 68564639 68564200 ID=Msa0136550-mRNA-1:exon:23649;Parent=Msa0136550-mRNA-1
chr1_3 exon 68565003 68565902 68565003 ID=Msa0136550-mRNA-1:exon:23650;Parent=Msa0136550-mRNA-1
chr1_3 five_prime_UTR 68562819 68562908 68562819 ID=Msa0136550-mRNA-1:five_prime_utr;Parent=Msa0136550-mRNA-1
chr1_3 CDS 68562909 68563000 68562909 ID=Msa0136550-mRNA-1:cds;Parent=Msa0136550-mRNA-1
chr1_3 CDS 68563139 68563252 68563139 ID=Msa0136550-mRNA-1:cds;Parent=Msa0136550-mRNA-1
chr1_3 CDS 68563414 68563641 68563414 ID=Msa0136550-mRNA-1:cds;Parent=Msa0136550-mRNA-1
chr1_3 CDS 68564200 68564639 68564200 ID=Msa0136550-mRNA-1:cds;Parent=Msa0136550-mRNA-1
chr1_3 CDS 68565003 68565205 68565003 ID=Msa0136550-mRNA-1:cds;Parent=Msa0136550-mRNA-1
chr1_3 three_prime_UTR 68565206 68565902 68565206 ID=Msa0136550-mRNA-1:three_prime_utr;Parent=Msa0136550-mRNA-1
Gene Sequence

>Msa0136550

ATGGGTAGCATTGAAGCTGCAGAAAGAACAACAGTAGGATTGGCTGCAAAAGACCCTTCCGGAATTCTCACTCCATACACATACACTCTCAGAAACACAGGACCGGATGATGTTTACATCAAAATTCATTACTGTGGAGTCTGTCATTCTGATCTTCACCAAATCAAAAATGATCTTGGCATGTCCAATTATCCTATGGTTCCTGGGCATGAAGTGGTTGGTGAGGTACTAGAGGTGGGTTCAAATGTTACAAGGTTCAAAGTGGGAGAAATTGTGGGAGTTGGTCTTCTCGTTGGTTGCTGCAAAAGCTGCAGAGCATGTGATTCTGAGATTGAGCAATACTGCAACAAGAAGATCTGGTCTTACAATGATGTTTACACTGACGGAAAGATCACTCAGGGTGGCTTTGCTGAATCCACCGTCGTTGAACAAAAGTTTGTTGTGAAAATACCAGAGGGATTGGCACCAGAGCAAGTTGCACCATTGTTGTGTGCAGGTGTGACTGTGTACAGTCCACTTTCACATTTTGGGCTGAAAACACCTGGGCTAAGAGGTGGAATATTGGGTCTTGGTGGAGTAGGACACATGGGGGTGAAAGTAGCAAAGGCATTAGGTCACCATGTCACTGTGATAAGCTCTTCTGATAAGAAGAAGAAAGAGGCACTTGAGGATCTTGGAGCTGACAATTATCTTGTTAGCTCAGATACTGTTGGGATGCAAGAAGCTGCTGATTCACTTGATTATATCATTGACACTGTTCCTGTTGGTCACCCTCTTGAGCCTTATCTTTCATTGCTCAAAATTGATGGGAAATTGATCTTGATGGGTGTTATTAACACCCCTCTTCAATTTGTTACACCTATGGTCATGCTAGGGAGGAAGTCAATTACTGGAAGCTTTGTTGGGAGCGTGAAGGAGACAGAGGAGATGTTGGAGTTTTGGAAAGAGAAGGGTCTAACTTCCATGATTGAAATTGTAACTATGGATTACATTAACAAAGCTTTTGAAAGATTGGAGAAGAATGATGTGAGGTATAGGTTCGTTGTGGATGTCAAAGGAAGTAAATTTGAGGAGTGA
Protein sequence

>Msa0136550

MGSIEAAERTTVGLAAKDPSGILTPYTYTLRNTGPDDVYIKIHYCGVCHSDLHQIKNDLGMSNYPMVPGHEVVGEVLEVGSNVTRFKVGEIVGVGLLVGCCKSCRACDSEIEQYCNKKIWSYNDVYTDGKITQGGFAESTVVEQKFVVKIPEGLAPEQVAPLLCAGVTVYSPLSHFGLKTPGLRGGILGLGGVGHMGVKVAKALGHHVTVISSSDKKKKEALEDLGADNYLVSSDTVGMQEAADSLDYIIDTVPVGHPLEPYLSLLKIDGKLILMGVINTPLQFVTPMVMLGRKSITGSFVGSVKETEEMLEFWKEKGLTSMIEIVTMDYINKAFERLEKNDVRYRFVVDVKGSKFEE*