AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1231810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1231810 MtrunA17_Chr8g0340731 83.150 273 38 2 1 267 1 271 2.11e-159 443
Msa1231810 MtrunA17_Chr2g0330951 50.545 275 110 7 4 267 6 265 2.67e-74 228
Msa1231810 MtrunA17_Chr5g0411501 43.506 154 72 3 103 256 181 319 1.05e-31 119
Msa1231810 MtrunA17_Chr6g0486121 35.409 257 136 8 3 256 9 238 4.29e-31 115
Msa1231810 MtrunA17_Chr5g0428141 44.805 154 67 5 103 256 196 331 1.99e-30 116
Msa1231810 MtrunA17_Chr1g0184791 39.394 165 87 2 89 253 75 226 2.81e-30 113
Msa1231810 MtrunA17_Chr1g0183251 38.764 178 78 7 103 256 82 252 3.70e-30 114
Msa1231810 MtrunA17_Chr8g0366881 41.935 155 72 3 103 256 195 332 3.85e-30 115
Msa1231810 MtrunA17_Chr1g0187921 37.952 166 63 4 103 256 83 220 7.08e-29 109
Msa1231810 MtrunA17_Chr2g0331411 41.558 154 75 3 103 256 172 310 7.32e-29 111
Msa1231810 MtrunA17_Chr8g0390281 39.610 154 77 3 103 256 140 277 1.03e-28 110
Msa1231810 MtrunA17_Chr1g0190711 42.581 155 74 5 103 256 97 237 4.60e-28 108
Msa1231810 MtrunA17_Chr1g0166011 37.267 161 59 4 103 263 67 185 3.37e-27 103
Msa1231810 MtrunA17_Chr7g0260621 38.312 154 56 4 103 256 83 197 1.54e-26 102
Msa1231810 MtrunA17_Chr1g0187961 37.662 154 54 4 103 256 63 174 2.32e-26 101
Msa1231810 MtrunA17_Chr1g0184801 30.078 256 110 7 5 256 7 197 6.28e-24 95.9
Msa1231810 MtrunA17_Chr7g0260641 37.086 151 77 4 103 253 99 231 2.65e-22 92.8
Msa1231810 MtrunA17_Chr4g0004181 33.129 163 64 3 103 265 54 171 1.69e-19 83.6
Msa1231810 MtrunA17_Chr4g0069561 37.143 105 50 1 151 255 94 182 5.09e-18 79.7
Msa1231810 MtrunA17_Chr1g0193861 34.259 108 56 1 146 253 78 170 2.48e-17 77.4
Msa1231810 MtrunA17_Chr7g0270851 37.255 102 49 1 152 253 69 155 2.53e-17 77.0
Msa1231810 MtrunA17_Chr2g0332141 36.893 103 58 1 153 255 8 103 4.58e-17 75.5
Msa1231810 MtrunA17_Chr7g0239061 32.143 112 54 1 154 265 573 662 7.80e-14 71.2
Msa1231810 MtrunA17_Chr3g0137601 26.752 157 83 4 100 256 85 209 1.96e-13 67.8
Msa1231810 MtrunA17_Chr1g0209181 39.604 101 44 2 153 253 85 168 3.96e-13 66.2
Msa1231810 MtrunA17_Chr4g0064131 26.974 152 72 5 104 254 93 206 1.15e-12 65.5
Msa1231810 MtrunA17_Chr8g0385791 27.536 138 70 4 129 264 693 802 7.56e-11 62.4
Msa1231810 MtrunA17_Chr8g0346711 30.769 104 50 1 154 257 561 642 8.18e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1231810 AT3G16500.1 45.788 273 106 8 2 266 25 263 2.98e-60 192
Msa1231810 AT3G16500.2 46.388 263 100 7 2 257 25 253 5.22e-60 191
Msa1231810 AT1G51950.1 44.528 265 99 7 2 257 26 251 4.07e-56 181
Msa1231810 AT5G25890.1 34.766 256 80 6 11 266 3 171 3.82e-34 122
Msa1231810 AT2G33310.2 42.500 160 78 4 103 256 76 227 1.17e-31 117
Msa1231810 AT2G33310.3 42.500 160 78 4 103 256 75 226 1.21e-31 117
Msa1231810 AT2G33310.1 42.500 160 78 4 103 256 75 226 1.21e-31 117
Msa1231810 AT4G28640.1 37.975 158 86 2 103 256 88 237 1.27e-30 115
Msa1231810 AT4G28640.3 37.975 158 86 2 103 256 88 237 2.22e-30 114
Msa1231810 AT4G28640.2 39.241 158 84 2 103 256 88 237 3.76e-30 115
Msa1231810 AT2G22670.5 42.581 155 72 5 103 256 165 303 4.09e-30 115
Msa1231810 AT2G22670.2 42.581 155 72 5 103 256 165 303 4.09e-30 115
Msa1231810 AT2G22670.3 42.581 155 72 5 103 256 165 303 4.38e-30 115
Msa1231810 AT2G22670.1 42.581 155 72 5 103 256 165 303 4.38e-30 115
Msa1231810 AT2G22670.4 42.581 155 72 5 103 256 182 320 6.25e-30 115
Msa1231810 AT4G29080.1 40.260 154 85 2 103 256 143 289 3.10e-29 112
Msa1231810 AT1G04240.1 36.646 161 66 4 103 263 64 188 1.80e-27 105
Msa1231810 AT1G04100.1 38.272 162 89 3 104 255 94 254 2.38e-27 106
Msa1231810 AT5G65670.2 38.710 155 77 4 103 256 183 320 2.48e-27 108
Msa1231810 AT5G65670.1 38.462 156 76 4 103 256 183 320 2.56e-27 108
Msa1231810 AT1G04240.2 36.646 161 66 4 103 263 164 288 8.14e-27 105
Msa1231810 AT1G04550.2 37.267 161 84 4 103 256 69 219 3.61e-26 103
Msa1231810 AT3G04730.1 41.667 156 84 4 103 256 70 220 6.20e-26 102
Msa1231810 AT1G04250.1 37.736 159 67 3 103 256 82 213 6.28e-26 102
Msa1231810 AT5G43700.1 37.267 161 63 4 103 263 62 184 6.64e-26 100
Msa1231810 AT4G14550.2 37.662 154 79 2 103 256 76 212 1.36e-25 101
Msa1231810 AT4G14550.1 37.662 154 79 2 103 256 76 212 1.39e-25 101
Msa1231810 AT3G23030.1 36.478 159 54 3 103 261 60 171 1.60e-24 97.1
Msa1231810 AT3G15540.1 36.810 163 58 5 103 262 70 190 4.95e-24 96.3
Msa1231810 AT3G23030.2 36.478 159 54 3 103 261 147 258 7.57e-24 97.4
Msa1231810 AT3G23050.1 34.302 172 68 4 103 256 82 226 1.21e-23 96.3
Msa1231810 AT2G46990.1 41.284 109 48 2 147 255 80 172 3.07e-20 85.5
Msa1231810 AT3G62100.1 40.741 108 48 2 147 254 78 169 7.93e-20 84.3
Msa1231810 AT1G52830.1 32.500 160 65 3 103 262 70 186 8.62e-20 84.7
Msa1231810 AT4G14550.3 35.766 137 71 2 103 239 76 195 7.50e-19 82.4
Msa1231810 AT3G17600.1 42.157 102 39 3 152 253 73 154 4.09e-18 79.7
Msa1231810 AT4G14560.1 39.252 107 49 2 153 259 76 166 4.36e-18 79.7
Msa1231810 AT3G23050.2 32.051 156 61 4 103 240 82 210 8.30e-17 77.4
Msa1231810 AT1G15580.1 33.333 153 56 5 103 255 53 159 1.89e-16 75.1
Msa1231810 AT3G62100.2 39.785 93 40 2 147 239 78 154 5.77e-13 65.5
Msa1231810 AT2G46990.2 39.362 94 41 2 146 239 79 156 7.43e-13 65.1
Msa1231810 AT2G01200.2 33.333 108 55 2 149 256 96 186 1.02e-11 62.8
Msa1231810 AT2G01200.1 33.333 108 55 2 149 256 96 186 1.02e-11 62.8
Msa1231810 AT1G15050.2 31.356 118 61 4 142 256 48 148 3.72e-11 60.5
Msa1231810 AT1G15050.1 31.356 118 61 4 142 256 80 180 5.39e-11 60.8
Msa1231810 AT4G14550.4 35.714 84 40 1 103 186 76 145 9.52e-11 60.1

Find 89 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGATAAACCTATCTTTA+AGG 0.110850 8_2:+8080899 None:intergenic
TTACATTCACACACAATATT+TGG 0.133960 8_2:+8077611 None:intergenic
ATCATTATTATAGGTTCTTC+TGG 0.198887 8_2:-8080883 Msa1231810:five_prime_UTR
TTGCATTCATACACATTATT+AGG 0.225646 8_2:+8077797 None:intergenic
TTTGGTTTCACACGCTTAAA+TGG 0.243122 8_2:+8081035 None:intergenic
TATAGCTTGGTCAACGATTC+AGG 0.244623 8_2:-8081215 Msa1231810:intron
CTTCATTAATGAAAATAAAA+AGG 0.250183 8_2:+8077588 None:intergenic
GATAGGTTTATCATTATTAT+AGG 0.254162 8_2:-8080892 Msa1231810:five_prime_UTR
ACTTGTTTCAATCTAGAAAA+AGG 0.257361 8_2:-8080817 Msa1231810:five_prime_UTR
CCTTTACCAGAGTTGCTAAT+AGG 0.291470 8_2:+8080008 None:intergenic
GAACAAATTCAAAGGAAATA+AGG 0.316814 8_2:+8080970 None:intergenic
AAGAAGCCACAAGTGTTCTT+TGG 0.323588 8_2:-8080748 Msa1231810:CDS
GGCCTCCGATTCGTTCATTC+AGG 0.338350 8_2:-8080118 Msa1231810:CDS
TAATCTACTTTAGCCCAAAA+AGG 0.354066 8_2:+8077840 None:intergenic
GAAATTCAGCTTTACCAAAC+AGG 0.360417 8_2:-8080480 Msa1231810:CDS
ATGATCAACGTTTACCTTCA+AGG 0.384045 8_2:+8079871 None:intergenic
AATATGGATGGAGTTCCTAT+TGG 0.394972 8_2:-8079969 Msa1231810:CDS
CTTCAAGGAGACCTCTAAAT+AGG 0.400379 8_2:+8079886 None:intergenic
GCTGTTGATGACCTATTTAG+AGG 0.418459 8_2:-8079897 Msa1231810:CDS
CTTGAGCTCAAGACTTGGAT+TGG 0.420807 8_2:-8077740 Msa1231810:three_prime_UTR
AAGCGTGTGAAACCAAAGTT+AGG 0.420992 8_2:-8081029 Msa1231810:five_prime_UTR
ATGGAATAGTTGCCTAACTT+TGG 0.430912 8_2:+8081017 None:intergenic
GAAGGAGACAAGATGCTTGT+AGG 0.431752 8_2:-8079135 Msa1231810:CDS
AAGCAAGAGGAAGAAGATAA+AGG 0.433944 8_2:-8079213 Msa1231810:CDS
AGCAGGAAGATCAGAACTCT+TGG 0.434060 8_2:+8078769 None:intergenic
TCATTATTATAGGTTCTTCT+GGG 0.438660 8_2:-8080882 Msa1231810:five_prime_UTR
CTATTTAGAGGTCTCCTTGA+AGG 0.443321 8_2:-8079885 Msa1231810:intron
TTATAGGTTCTTCTGGGAGG+TGG 0.456111 8_2:-8080876 Msa1231810:five_prime_UTR
AAAGGATCATTGACTGGAAG+TGG 0.456184 8_2:-8079186 Msa1231810:CDS
ATATGGATGGAGTTCCTATT+GGG 0.456891 8_2:-8079968 Msa1231810:CDS
TCATTAATGAAGATTTAAGT+TGG 0.456981 8_2:-8077577 Msa1231810:three_prime_UTR
GCTGAATTTCTTGCAGAAGA+AGG 0.458077 8_2:+8080493 None:intergenic
GCCTACTGAGTCCCGGCCTA+TGG 0.458539 8_2:-8080063 Msa1231810:CDS
CTATGATACAGATGTCTCTT+CGG 0.468482 8_2:+8081378 None:intergenic
CTTGTTTGTGAAGATCAATA+TGG 0.475066 8_2:-8079985 Msa1231810:CDS
AAGGAGACAAGATGCTTGTA+GGG 0.476173 8_2:-8079134 Msa1231810:CDS
TTCTTTGGAGGAGAGGAAGT+TGG 0.476777 8_2:-8080733 Msa1231810:CDS
ATTGACATCAATGCTTATGA+TGG 0.483130 8_2:-8079939 Msa1231810:CDS
CTTGTTTATGAAGATAATGA+AGG 0.488627 8_2:-8079153 Msa1231810:CDS
CTTTGCGTTGCCGAGTCAAG+TGG 0.489355 8_2:+8077923 None:intergenic
CCTATTAGCAACTCTGGTAA+AGG 0.491874 8_2:-8080008 Msa1231810:CDS
TACCAAACAGGTCTCAGAAA+AGG 0.493666 8_2:-8080468 Msa1231810:intron
CATTGATTAAAGTTATAGCT+TGG 0.495869 8_2:-8081228 Msa1231810:five_prime_UTR
GAAATACTAAAACAATTAAA+CGG 0.499774 8_2:-8081307 Msa1231810:five_prime_UTR
GAAGAAGATAAAGGCATCAA+AGG 0.503044 8_2:-8079204 Msa1231810:CDS
GCAGAGATAAATTTATCTCA+TGG 0.505424 8_2:-8079246 Msa1231810:intron
GAGCTTAGACTTGGTCCACC+TGG 0.509843 8_2:-8080711 Msa1231810:CDS
ATGATATTTGATGCATCACT+AGG 0.521075 8_2:-8077652 Msa1231810:three_prime_UTR
AGGAAGGATGAGGCTGTTTG+AGG 0.522837 8_2:-8077902 Msa1231810:three_prime_UTR
GAAGAATCATCACTCACTCT+TGG 0.524837 8_2:-8080633 Msa1231810:CDS
GGCATCAAAGGATCATTGAC+TGG 0.528381 8_2:-8079192 Msa1231810:CDS
CTGCTGGTCCAGTAGTAGGT+TGG 0.531744 8_2:-8080139 Msa1231810:intron
AAGCCACAAGTGTTCTTTGG+AGG 0.532124 8_2:-8080745 Msa1231810:CDS
TTCTTCCTGAATGAACGAAT+CGG 0.538928 8_2:+8080113 None:intergenic
AGGAAGTTGGAGCTTAGACT+TGG 0.541596 8_2:-8080720 Msa1231810:CDS
ACAAGTGTTCTTTGGAGGAG+AGG 0.544453 8_2:-8080740 Msa1231810:CDS
TGCTTGTAGGGGACGTGCCG+TGG 0.551530 8_2:-8079122 Msa1231810:CDS
ATATCCGTGTGCTGAAAGCT+TGG 0.551850 8_2:-8077533 Msa1231810:three_prime_UTR
TTGACAAACTCTTCTTCACC+AGG 0.560561 8_2:+8080693 None:intergenic
AAATCCAAGCTTTCAGCACA+CGG 0.560697 8_2:+8077529 None:intergenic
CCTTGTCTTGAACCATAGGC+CGG 0.561981 8_2:+8080051 None:intergenic
CCGGCCTATGGTTCAAGACA+AGG 0.569410 8_2:-8080051 Msa1231810:CDS
TTCAGGAAGAACATTGCAAG+TGG 0.570187 8_2:-8080101 Msa1231810:CDS
TCTTGCAAGTTTGTGCAACA+AGG 0.574470 8_2:+8080538 None:intergenic
CTATTCCATGCTATATTCTG+TGG 0.576490 8_2:-8081003 Msa1231810:five_prime_UTR
ATAACCTTGTCTTGAACCAT+AGG 0.576939 8_2:+8080047 None:intergenic
ATGTTCTTGAGCTCAAGACT+TGG 0.578024 8_2:-8077745 Msa1231810:three_prime_UTR
CACTTGACTCGGCAACGCAA+AGG 0.584528 8_2:-8077922 Msa1231810:three_prime_UTR
ATACATTAGACCACTTGACT+CGG 0.590858 8_2:-8077933 Msa1231810:three_prime_UTR
TGGAATTAACAAGAAGCAAG+AGG 0.591043 8_2:-8079226 Msa1231810:CDS
AGTGAACAGAACAAATTCAA+AGG 0.595215 8_2:+8080962 None:intergenic
CTAAGCCGCCTACTGAGTCC+CGG 0.597620 8_2:-8080070 Msa1231810:CDS
CTTGTCTTGAACCATAGGCC+GGG 0.603934 8_2:+8080052 None:intergenic
TTTGTGAAGATCAATATGGA+TGG 0.610573 8_2:-8079981 Msa1231810:CDS
TATATATTCTACTAATGTAG+AGG 0.613994 8_2:-8077691 Msa1231810:three_prime_UTR
TGATAAGATACTTACTGCCA+CGG 0.622281 8_2:+8079105 None:intergenic
ATGAGCCACAGAATATAGCA+TGG 0.623301 8_2:+8080998 None:intergenic
TTATTATAGGTTCTTCTGGG+AGG 0.624893 8_2:-8080879 Msa1231810:five_prime_UTR
TGACTCGGCAACGCAAAGGA+AGG 0.625889 8_2:-8077918 Msa1231810:three_prime_UTR
GGCAACGCAAAGGAAGGATG+AGG 0.628215 8_2:-8077912 Msa1231810:three_prime_UTR
TGTTTGTATCAACAGTGAAG+AGG 0.629944 8_2:-8078802 Msa1231810:CDS
TGTTGCACAAACTTGCAAGA+TGG 0.633974 8_2:-8080535 Msa1231810:CDS
AAGACTTGGATTGGATGCTG+AGG 0.634139 8_2:-8077731 Msa1231810:three_prime_UTR
TCTCCTCCAAAGAACACTTG+TGG 0.635664 8_2:+8080742 None:intergenic
TGATATTTGATGCATCACTA+GGG 0.637267 8_2:-8077651 Msa1231810:three_prime_UTR
ATAGGCCGGGACTCAGTAGG+CGG 0.638385 8_2:+8080065 None:intergenic
TTCCTGAATGAACGAATCGG+AGG 0.657416 8_2:+8080116 None:intergenic
ACAAACTCTTCTTCACCAGG+TGG 0.675573 8_2:+8080696 None:intergenic
AGGAGACAAGATGCTTGTAG+GGG 0.714162 8_2:-8079133 Msa1231810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTTAATATGTTTGTT+TGG - chr8_2:8080080-8080099 Msa1231810:CDS 10.0%
!! GAAATACTAAAACAATTAAA+CGG - chr8_2:8077603-8077622 Msa1231810:three_prime_UTR 15.0%
!!! GTATATTTTTAATGATCTAA+CGG + chr8_2:8079538-8079557 None:intergenic 15.0%
!! TTAAAACAAAATTCACTTAA+AGG + chr8_2:8079597-8079616 None:intergenic 15.0%
!!! ACTATAGTTTTAAAAAACAA+AGG + chr8_2:8079926-8079945 None:intergenic 15.0%
!! AATGATTTAGTTATGATAAT+TGG - chr8_2:8080031-8080050 Msa1231810:CDS 15.0%
!! TACAAATTAAAGAGTAATTA+GGG - chr8_2:8080261-8080280 Msa1231810:intron 15.0%
!! CTTCATTAATGAAAATAAAA+AGG + chr8_2:8081325-8081344 None:intergenic 15.0%
!!! TTTCAATTACATTTTTGTCT+CGG + chr8_2:8077587-8077606 None:intergenic 20.0%
!! CAATGTTATAAAAACTGATT+TGG + chr8_2:8077667-8077686 None:intergenic 20.0%
!! TAATGATAAACCTATCTTTA+AGG + chr8_2:8078014-8078033 None:intergenic 20.0%
!! GATAGGTTTATCATTATTAT+AGG - chr8_2:8078018-8078037 Msa1231810:intron 20.0%
!! ATAAAGATGTAAATTGTTGT+TGG - chr8_2:8078562-8078581 Msa1231810:intron 20.0%
!!! TTCACCAAACAAATATTTTA+AGG - chr8_2:8079458-8079477 Msa1231810:intron 20.0%
!! TTGTCCTTAAAATATTTGTT+TGG + chr8_2:8079465-8079484 None:intergenic 20.0%
!!! AACAAAGGTTAATTTTACAT+TGG + chr8_2:8079911-8079930 None:intergenic 20.0%
!! AAAAACTCTTAGTTATTGTA+AGG + chr8_2:8080224-8080243 None:intergenic 20.0%
!! GTACAAATTAAAGAGTAATT+AGG - chr8_2:8080260-8080279 Msa1231810:intron 20.0%
!! AATTGCAAATGAAATTTATG+AGG - chr8_2:8080437-8080456 Msa1231810:intron 20.0%
!! GTAAAATTTATTTACGTGTT+TGG - chr8_2:8080459-8080478 Msa1231810:intron 20.0%
!! TTCAATAGTTCATAAAATTC+AGG - chr8_2:8080665-8080684 Msa1231810:CDS 20.0%
!! ATCACAACTAAAATAAATCA+GGG + chr8_2:8080903-8080922 None:intergenic 20.0%
!! TATATATTCTACTAATGTAG+AGG - chr8_2:8081219-8081238 Msa1231810:five_prime_UTR 20.0%
!! TCATTAATGAAGATTTAAGT+TGG - chr8_2:8081333-8081352 Msa1231810:five_prime_UTR 20.0%
!!! GTTATAAAAACTGATTTGGA+CGG + chr8_2:8077663-8077682 None:intergenic 25.0%
!! CATTGATTAAAGTTATAGCT+TGG - chr8_2:8077682-8077701 Msa1231810:three_prime_UTR 25.0%
! TAACAAACCAAAAACAGAAA+GGG + chr8_2:8077793-8077812 None:intergenic 25.0%
!! TCTTTTTCTTTCCTCTTTTT+AGG + chr8_2:8077854-8077873 None:intergenic 25.0%
! GAACAAATTCAAAGGAAATA+AGG + chr8_2:8077943-8077962 None:intergenic 25.0%
! ATCATTATTATAGGTTCTTC+TGG - chr8_2:8078027-8078046 Msa1231810:intron 25.0%
! TCATTATTATAGGTTCTTCT+GGG - chr8_2:8078028-8078047 Msa1231810:intron 25.0%
! ACTTGTTTCAATCTAGAAAA+AGG - chr8_2:8078093-8078112 Msa1231810:intron 25.0%
! GACACAATTATTGCATTAAA+AGG + chr8_2:8079200-8079219 None:intergenic 25.0%
!! TGGAAATGTTTTTCTTTGTT+GGG - chr8_2:8079282-8079301 Msa1231810:intron 25.0%
! TTTCAATCATAACTACTTCT+AGG - chr8_2:8079568-8079587 Msa1231810:intron 25.0%
! CTTGTTTATGAAGATAATGA+AGG - chr8_2:8079757-8079776 Msa1231810:intron 25.0%
! AAAACTCACACAATAACATA+GGG + chr8_2:8079999-8080018 None:intergenic 25.0%
!! TTAACAAGTACTTCTAAAGA+AGG - chr8_2:8080187-8080206 Msa1231810:intron 25.0%
!! TAACAAGTACTTCTAAAGAA+GGG - chr8_2:8080188-8080207 Msa1231810:intron 25.0%
! GATCACAACTAAAATAAATC+AGG + chr8_2:8080904-8080923 None:intergenic 25.0%
!!! AGTGTTTTTGTTTGACATTT+TGG - chr8_2:8081045-8081064 Msa1231810:five_prime_UTR 25.0%
! TTGCATTCATACACATTATT+AGG + chr8_2:8081116-8081135 None:intergenic 25.0%
! TTACATTCACACACAATATT+TGG + chr8_2:8081302-8081321 None:intergenic 25.0%
AAAAACAGAAAGGGAATTAG+TGG + chr8_2:8077784-8077803 None:intergenic 30.0%
! ACTAATTCCCTTTCTGTTTT+TGG - chr8_2:8077783-8077802 Msa1231810:three_prime_UTR 30.0%
CTAACAAACCAAAAACAGAA+AGG + chr8_2:8077794-8077813 None:intergenic 30.0%
!!! CTTTCTGTTTTTGGTTTGTT+AGG - chr8_2:8077792-8077811 Msa1231810:three_prime_UTR 30.0%
AGTGAACAGAACAAATTCAA+AGG + chr8_2:8077951-8077970 None:intergenic 30.0%
! CAAAGTTTTTCACTTCTTGA+TGG - chr8_2:8077980-8077999 Msa1231810:intron 30.0%
!! GGTTTTTAAGCCTTAAAGAT+AGG - chr8_2:8078001-8078020 Msa1231810:intron 30.0%
! TTTTTGTAAGCAACAAGATG+TGG - chr8_2:8078137-8078156 Msa1231810:intron 30.0%
GATATAAGAGAGAAAAGCAA+AGG + chr8_2:8078472-8078491 None:intergenic 30.0%
CTCTCTTATATCTATGTTTG+TGG - chr8_2:8078480-8078499 Msa1231810:intron 30.0%
TATCAAGAACAACATTCACT+CGG + chr8_2:8078538-8078557 None:intergenic 30.0%
AAAAAACCTATTAGCAACTC+TGG - chr8_2:8078896-8078915 Msa1231810:intron 30.0%
CTTGTTTGTGAAGATCAATA+TGG - chr8_2:8078925-8078944 Msa1231810:intron 30.0%
TTTGTGAAGATCAATATGGA+TGG - chr8_2:8078929-8078948 Msa1231810:intron 30.0%
! ATGTCAATTTTTCTCCCAAT+AGG + chr8_2:8078959-8078978 None:intergenic 30.0%
ATTGACATCAATGCTTATGA+TGG - chr8_2:8078971-8078990 Msa1231810:intron 30.0%
!! GTCTGATTTTAATCTATCGA+CGG - chr8_2:8079117-8079136 Msa1231810:intron 30.0%
AATTATTGCATTAAAAGGCC+AGG + chr8_2:8079195-8079214 None:intergenic 30.0%
!! TGACATAACTTTTTGTTGTG+TGG - chr8_2:8079262-8079281 Msa1231810:intron 30.0%
! GTGGAAATGTTTTTCTTTGT+TGG - chr8_2:8079281-8079300 Msa1231810:intron 30.0%
CAAAAGATGTAGGTAACAAA+AGG + chr8_2:8079342-8079361 None:intergenic 30.0%
GCTATTTAGTCAAAAGATGT+AGG + chr8_2:8079352-8079371 None:intergenic 30.0%
!!! GTTTCAATTGCATTTTAGCT+AGG + chr8_2:8079410-8079429 None:intergenic 30.0%
GCAGAGATAAATTTATCTCA+TGG - chr8_2:8079664-8079683 Msa1231810:intron 30.0%
CAAAACTCACACAATAACAT+AGG + chr8_2:8080000-8080019 None:intergenic 30.0%
AAAAACTCACTGTTAAAAGC+AGG + chr8_2:8080161-8080180 None:intergenic 30.0%
! AACAAGTACTTCTAAAGAAG+GGG - chr8_2:8080189-8080208 Msa1231810:intron 30.0%
GCTTAGTCATAAATTGTAAC+AGG - chr8_2:8080308-8080327 Msa1231810:intron 30.0%
GTGACATGTTAATGAATATG+TGG - chr8_2:8080379-8080398 Msa1231810:intron 30.0%
! TTTAGTCCTTAATGACTTGT+GGG + chr8_2:8080419-8080438 None:intergenic 30.0%
ATTTAGTCCTTAATGACTTG+TGG + chr8_2:8080420-8080439 None:intergenic 30.0%
AAATATTGTGATGTAGAAGC+TGG + chr8_2:8080753-8080772 None:intergenic 30.0%
TAAATGTTCTTGTTCACAGT+TGG - chr8_2:8080934-8080953 Msa1231810:five_prime_UTR 30.0%
!!! TTGACATTTTGGTTCCTTTT+TGG - chr8_2:8081056-8081075 Msa1231810:five_prime_UTR 30.0%
!!! TGACATTTTGGTTCCTTTTT+GGG - chr8_2:8081057-8081076 Msa1231810:five_prime_UTR 30.0%
TAATCTACTTTAGCCCAAAA+AGG + chr8_2:8081073-8081092 None:intergenic 30.0%
!! TTTTTGGGCTAAAGTAGATT+AGG - chr8_2:8081072-8081091 Msa1231810:five_prime_UTR 30.0%
! TTTTGGGCTAAAGTAGATTA+GGG - chr8_2:8081073-8081092 Msa1231810:five_prime_UTR 30.0%
ATGATATTTGATGCATCACT+AGG - chr8_2:8081258-8081277 Msa1231810:five_prime_UTR 30.0%
TGATATTTGATGCATCACTA+GGG - chr8_2:8081259-8081278 Msa1231810:five_prime_UTR 30.0%
!! CATTTTTGTCTCGGTTTGAA+TGG + chr8_2:8077578-8077597 None:intergenic 35.0%
CGCAACTTGAAACAAATGTT+AGG + chr8_2:8077753-8077772 None:intergenic 35.0%
!!! GTTTTTGGTTTGTTAGGCTA+AGG - chr8_2:8077798-8077817 Msa1231810:three_prime_UTR 35.0%
TCAAACCTATGCCTAAAAAG+AGG - chr8_2:8077840-8077859 Msa1231810:three_prime_UTR 35.0%
!! TCTTTCCTCTTTTTAGGCAT+AGG + chr8_2:8077848-8077867 None:intergenic 35.0%
! TTTGGTTTCACACGCTTAAA+TGG + chr8_2:8077878-8077897 None:intergenic 35.0%
ATGGAATAGTTGCCTAACTT+TGG + chr8_2:8077896-8077915 None:intergenic 35.0%
CTATTCCATGCTATATTCTG+TGG - chr8_2:8077907-8077926 Msa1231810:three_prime_UTR 35.0%
TTATTATAGGTTCTTCTGGG+AGG - chr8_2:8078031-8078050 Msa1231810:intron 35.0%
GAAATTCAGCTTTACCAAAC+AGG - chr8_2:8078430-8078449 Msa1231810:intron 35.0%
!!! AGCAGCATTTCTGTTTTAGT+TGG + chr8_2:8078756-8078775 None:intergenic 35.0%
AACTAAAACAGAAATGCTGC+TGG - chr8_2:8078755-8078774 Msa1231810:intron 35.0%
TTCTTCCTGAATGAACGAAT+CGG + chr8_2:8078800-8078819 None:intergenic 35.0%
ATAACCTTGTCTTGAACCAT+AGG + chr8_2:8078866-8078885 None:intergenic 35.0%
AATATGGATGGAGTTCCTAT+TGG - chr8_2:8078941-8078960 Msa1231810:intron 35.0%
ATATGGATGGAGTTCCTATT+GGG - chr8_2:8078942-8078961 Msa1231810:intron 35.0%
ATGATCAACGTTTACCTTCA+AGG + chr8_2:8079042-8079061 None:intergenic 35.0%
ATCTAACTCAATGTCTACCA+TGG - chr8_2:8079144-8079163 Msa1231810:CDS 35.0%
TGACTGTAGTAAAAATGCCA+TGG + chr8_2:8079164-8079183 None:intergenic 35.0%
CAAGTAACATTCAAAACTCC+TGG - chr8_2:8079371-8079390 Msa1231810:intron 35.0%
!! CTAGGTTGACATTTTATACC+AGG + chr8_2:8079392-8079411 None:intergenic 35.0%
ATGATTGATTGACAGTGCAA+CGG + chr8_2:8079513-8079532 None:intergenic 35.0%
CAATCATAACTACTTCTAGG+TGG - chr8_2:8079571-8079590 Msa1231810:intron 35.0%
TGGAATTAACAAGAAGCAAG+AGG - chr8_2:8079684-8079703 Msa1231810:intron 35.0%
AAGCAAGAGGAAGAAGATAA+AGG - chr8_2:8079697-8079716 Msa1231810:intron 35.0%
!! GAAGAAGATAAAGGCATCAA+AGG - chr8_2:8079706-8079725 Msa1231810:intron 35.0%
TGATAAGATACTTACTGCCA+CGG + chr8_2:8079808-8079827 None:intergenic 35.0%
CACACAATAACATAGGGAAA+AGG + chr8_2:8079993-8080012 None:intergenic 35.0%
!! AGCTATGATTTGTTACTGTG+TGG - chr8_2:8080055-8080074 Msa1231810:CDS 35.0%
TGTTTGTATCAACAGTGAAG+AGG - chr8_2:8080108-8080127 Msa1231810:CDS 35.0%
CTGTTAACTTATGTCGTTAG+AGG + chr8_2:8080616-8080635 None:intergenic 35.0%
ATACATTAGACCACTTGACT+CGG - chr8_2:8080977-8080996 Msa1231810:five_prime_UTR 35.0%
TATAGCTTGGTCAACGATTC+AGG - chr8_2:8077695-8077714 Msa1231810:three_prime_UTR 40.0%
AAGCGTGTGAAACCAAAGTT+AGG - chr8_2:8077881-8077900 Msa1231810:three_prime_UTR 40.0%
ATGAGCCACAGAATATAGCA+TGG + chr8_2:8077915-8077934 None:intergenic 40.0%
AAGAAGCCACAAGTGTTCTT+TGG - chr8_2:8078162-8078181 Msa1231810:intron 40.0%
TTGACAAACTCTTCTTCACC+AGG + chr8_2:8078220-8078239 None:intergenic 40.0%
GAAGAATCATCACTCACTCT+TGG - chr8_2:8078277-8078296 Msa1231810:intron 40.0%
TCTTGCAAGTTTGTGCAACA+AGG + chr8_2:8078375-8078394 None:intergenic 40.0%
TGTTGCACAAACTTGCAAGA+TGG - chr8_2:8078375-8078394 Msa1231810:intron 40.0%
GCTGAATTTCTTGCAGAAGA+AGG + chr8_2:8078420-8078439 None:intergenic 40.0%
TACCAAACAGGTCTCAGAAA+AGG - chr8_2:8078442-8078461 Msa1231810:intron 40.0%
!!! TACCTTTTCTGAGACCTGTT+TGG + chr8_2:8078447-8078466 None:intergenic 40.0%
ATACCAAAACCGTAACGTTG+TGG - chr8_2:8078622-8078641 Msa1231810:intron 40.0%
CGTATCTGCAACCACAATTA+CGG + chr8_2:8078648-8078667 None:intergenic 40.0%
TTCAGGAAGAACATTGCAAG+TGG - chr8_2:8078809-8078828 Msa1231810:intron 40.0%
CCTTTACCAGAGTTGCTAAT+AGG + chr8_2:8078905-8078924 None:intergenic 40.0%
CCTATTAGCAACTCTGGTAA+AGG - chr8_2:8078902-8078921 Msa1231810:intron 40.0%
!! GCTGTTGATGACCTATTTAG+AGG - chr8_2:8079013-8079032 Msa1231810:intron 40.0%
CTTCAAGGAGACCTCTAAAT+AGG + chr8_2:8079027-8079046 None:intergenic 40.0%
CTATTTAGAGGTCTCCTTGA+AGG - chr8_2:8079025-8079044 Msa1231810:intron 40.0%
! AAAGGATCATTGACTGGAAG+TGG - chr8_2:8079724-8079743 Msa1231810:intron 40.0%
AAGGAGACAAGATGCTTGTA+GGG - chr8_2:8079776-8079795 Msa1231810:intron 40.0%
TTGTAACAGGTCAGATCAAG+TGG - chr8_2:8080321-8080340 Msa1231810:intron 40.0%
GGTCTGTCTAGTAACTTATG+TGG - chr8_2:8080342-8080361 Msa1231810:intron 40.0%
GTCAAACAAAAACACTCGTG+TGG + chr8_2:8081041-8081060 None:intergenic 40.0%
ATGTTCTTGAGCTCAAGACT+TGG - chr8_2:8081165-8081184 Msa1231810:intron 40.0%
! CTTGGTCAACGATTCAGGTT+AGG - chr8_2:8077700-8077719 Msa1231810:three_prime_UTR 45.0%
! TTATAGGTTCTTCTGGGAGG+TGG - chr8_2:8078034-8078053 Msa1231810:intron 45.0%
AAGCCACAAGTGTTCTTTGG+AGG - chr8_2:8078165-8078184 Msa1231810:intron 45.0%
TCTCCTCCAAAGAACACTTG+TGG + chr8_2:8078171-8078190 None:intergenic 45.0%
! ACAAGTGTTCTTTGGAGGAG+AGG - chr8_2:8078170-8078189 Msa1231810:intron 45.0%
! TTCTTTGGAGGAGAGGAAGT+TGG - chr8_2:8078177-8078196 Msa1231810:intron 45.0%
AGGAAGTTGGAGCTTAGACT+TGG - chr8_2:8078190-8078209 Msa1231810:intron 45.0%
ACAAACTCTTCTTCACCAGG+TGG + chr8_2:8078217-8078236 None:intergenic 45.0%
CAAGAACAACATTCACTCGG+CGG + chr8_2:8078535-8078554 None:intergenic 45.0%
CAATTACGGCCACAACGTTA+CGG + chr8_2:8078634-8078653 None:intergenic 45.0%
TAACGTTGTGGCCGTAATTG+TGG - chr8_2:8078634-8078653 Msa1231810:intron 45.0%
TTCCTGAATGAACGAATCGG+AGG + chr8_2:8078797-8078816 None:intergenic 45.0%
ACTACAGTCAATCCAGATCC+TGG - chr8_2:8079174-8079193 Msa1231810:CDS 45.0%
TGCATTAAAAGGCCAGGATC+TGG + chr8_2:8079189-8079208 None:intergenic 45.0%
GGCATCAAAGGATCATTGAC+TGG - chr8_2:8079718-8079737 Msa1231810:intron 45.0%
GAAGGAGACAAGATGCTTGT+AGG - chr8_2:8079775-8079794 Msa1231810:intron 45.0%
AGGAGACAAGATGCTTGTAG+GGG - chr8_2:8079777-8079796 Msa1231810:intron 45.0%
AGCAGGAAGATCAGAACTCT+TGG + chr8_2:8080144-8080163 None:intergenic 45.0%
ACTTGTGGGATGCACATATG+AGG + chr8_2:8080405-8080424 None:intergenic 45.0%
GTGCATCCCACAAGTCATTA+AGG - chr8_2:8080410-8080429 Msa1231810:intron 45.0%
CTTGAGCTCAAGACTTGGAT+TGG - chr8_2:8081170-8081189 Msa1231810:intron 45.0%
! AAGACTTGGATTGGATGCTG+AGG - chr8_2:8081179-8081198 Msa1231810:intron 45.0%
AATGCTGCTGGTCCAGTAGT+AGG - chr8_2:8078767-8078786 Msa1231810:CDS 50.0%
CTTGTCTTGAACCATAGGCC+GGG + chr8_2:8078861-8078880 None:intergenic 50.0%
CCTTGTCTTGAACCATAGGC+CGG + chr8_2:8078862-8078881 None:intergenic 50.0%
AGGAAGGATGAGGCTGTTTG+AGG - chr8_2:8081008-8081027 Msa1231810:five_prime_UTR 50.0%
! GAGCTTAGACTTGGTCCACC+TGG - chr8_2:8078199-8078218 Msa1231810:intron 55.0%
! CGGCCACAACGTTACGGTTT+TGG + chr8_2:8078628-8078647 None:intergenic 55.0%
CTGCTGGTCCAGTAGTAGGT+TGG - chr8_2:8078771-8078790 Msa1231810:CDS 55.0%
ATCGGAGGCCAACCTACTAC+TGG + chr8_2:8078782-8078801 None:intergenic 55.0%
! GGCCTCCGATTCGTTCATTC+AGG - chr8_2:8078792-8078811 Msa1231810:CDS 55.0%
CCGGCCTATGGTTCAAGACA+AGG - chr8_2:8078859-8078878 Msa1231810:intron 55.0%
CTTTGCGTTGCCGAGTCAAG+TGG + chr8_2:8080990-8081009 None:intergenic 55.0%
!! CACTTGACTCGGCAACGCAA+AGG - chr8_2:8080988-8081007 Msa1231810:five_prime_UTR 55.0%
! TGACTCGGCAACGCAAAGGA+AGG - chr8_2:8080992-8081011 Msa1231810:five_prime_UTR 55.0%
GGCAACGCAAAGGAAGGATG+AGG - chr8_2:8080998-8081017 Msa1231810:five_prime_UTR 55.0%
CTAAGCCGCCTACTGAGTCC+CGG - chr8_2:8078840-8078859 Msa1231810:intron 60.0%
ATAGGCCGGGACTCAGTAGG+CGG + chr8_2:8078848-8078867 None:intergenic 60.0%
ACCATAGGCCGGGACTCAGT+AGG + chr8_2:8078851-8078870 None:intergenic 60.0%
GCCTACTGAGTCCCGGCCTA+TGG - chr8_2:8078847-8078866 Msa1231810:intron 65.0%
TGCTTGTAGGGGACGTGCCG+TGG - chr8_2:8079788-8079807 Msa1231810:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 8077533 8081399 8077533 ID=Msa1231810;Name=Msa1231810
chr8_2 mRNA 8077533 8081399 8077533 ID=Msa1231810-mRNA-1;Parent=Msa1231810;Name=Msa1231810-mRNA-1;_AED=0.03;_eAED=0.03;_QI=524|0.8|0.83|1|0.8|0.66|6|427|267
chr8_2 exon 8077533 8077979 8077533 ID=Msa1231810-mRNA-1:exon:3063;Parent=Msa1231810-mRNA-1
chr8_2 exon 8078762 8078826 8078762 ID=Msa1231810-mRNA-1:exon:3062;Parent=Msa1231810-mRNA-1
chr8_2 exon 8079120 8079264 8079120 ID=Msa1231810-mRNA-1:exon:3061;Parent=Msa1231810-mRNA-1
chr8_2 exon 8079886 8080149 8079886 ID=Msa1231810-mRNA-1:exon:3060;Parent=Msa1231810-mRNA-1
chr8_2 exon 8080469 8081118 8080469 ID=Msa1231810-mRNA-1:exon:3059;Parent=Msa1231810-mRNA-1
chr8_2 exon 8081216 8081399 8081216 ID=Msa1231810-mRNA-1:exon:3058;Parent=Msa1231810-mRNA-1
chr8_2 five_prime_UTR 8081216 8081399 8081216 ID=Msa1231810-mRNA-1:five_prime_utr;Parent=Msa1231810-mRNA-1
chr8_2 five_prime_UTR 8080779 8081118 8080779 ID=Msa1231810-mRNA-1:five_prime_utr;Parent=Msa1231810-mRNA-1
chr8_2 CDS 8080469 8080778 8080469 ID=Msa1231810-mRNA-1:cds;Parent=Msa1231810-mRNA-1
chr8_2 CDS 8079886 8080149 8079886 ID=Msa1231810-mRNA-1:cds;Parent=Msa1231810-mRNA-1
chr8_2 CDS 8079120 8079264 8079120 ID=Msa1231810-mRNA-1:cds;Parent=Msa1231810-mRNA-1
chr8_2 CDS 8078762 8078826 8078762 ID=Msa1231810-mRNA-1:cds;Parent=Msa1231810-mRNA-1
chr8_2 CDS 8077960 8077979 8077960 ID=Msa1231810-mRNA-1:cds;Parent=Msa1231810-mRNA-1
chr8_2 three_prime_UTR 8077533 8077959 8077533 ID=Msa1231810-mRNA-1:three_prime_utr;Parent=Msa1231810-mRNA-1
Gene Sequence

>Msa1231810

ATGTGGCAAAGAAGCCACAAGTGTTCTTTGGAGGAGAGGAAGTTGGAGCTTAGACTTGGTCCACCTGGTGAAGAAGAGTTTGTCAATGAAAAAAGCTTGAAGAATGTCAAAAAAGAAAGAGATGAAGAATCATCACTCACTCTTGGTTATGCTAAAGATTCATCATTTCTTAAGCTCTCATCAATGCAAGTGATGAAAAAGATGCATCACAATCACAACCTTGTTGCACAAACTTGCAAGATGGTGAGAAAAATGCATTCTCTCCTTCTTCTGCAAGAAATTCAGCTTTACCAAACAGGTCTCAGAAAAGTAGTAGGTTGGCCTCCGATTCGTTCATTCAGGAAGAACATTGCAAGTGGAAGCTCTTCTAAGCCGCCTACTGAGTCCCGGCCTATGGTTCAAGACAAGGTTATTGTTGATAGTAAAAAACCTATTAGCAACTCTGGTAAAGGCTTGTTTGTGAAGATCAATATGGATGGAGTTCCTATTGGGAGAAAAATTGACATCAATGCTTATGATGGCTATGAAAAACTTTCATCTGCTGTTGATGACCTATTTAGAGGTCTCCTTGAAGAGATAAATTTATCTCATGGAATTAACAAGAAGCAAGAGGAAGAAGATAAAGGCATCAAAGGATCATTGACTGGAAGTGGAGAATATACTCTTGTTTATGAAGATAATGAAGGAGACAAGATGCTTGTAGGGGACGTGCCGTGGCACATGTTTGTATCAACAGTGAAGAGGCTGCGTGTGTCCAAGAGTTCTGATCTTCCTGCTTTTAACATTGGTAGCAAGAAAGACTAG
Protein sequence

>Msa1231810

MWQRSHKCSLEERKLELRLGPPGEEEFVNEKSLKNVKKERDEESSLTLGYAKDSSFLKLSSMQVMKKMHHNHNLVAQTCKMVRKMHSLLLLQEIQLYQTGLRKVVGWPPIRSFRKNIASGSSSKPPTESRPMVQDKVIVDSKKPISNSGKGLFVKINMDGVPIGRKIDINAYDGYEKLSSAVDDLFRGLLEEINLSHGINKKQEEEDKGIKGSLTGSGEYTLVYEDNEGDKMLVGDVPWHMFVSTVKRLRVSKSSDLPAFNIGSKKD*