AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1312500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1312500 MtrunA17_Chr8g0340731 95.604 273 10 1 1 273 1 271 0.0 530
Msa1312500 MtrunA17_Chr2g0330951 55.036 278 99 8 4 273 6 265 2.85e-85 256
Msa1312500 MtrunA17_Chr6g0486121 35.581 267 128 9 3 262 9 238 2.90e-33 122
Msa1312500 MtrunA17_Chr8g0366881 38.776 196 98 5 70 262 156 332 5.85e-33 123
Msa1312500 MtrunA17_Chr5g0411501 40.331 181 86 4 89 262 154 319 6.75e-33 122
Msa1312500 MtrunA17_Chr1g0183251 38.418 177 88 4 105 262 78 252 8.38e-31 115
Msa1312500 MtrunA17_Chr5g0428141 45.098 153 66 5 110 262 197 331 8.71e-31 117
Msa1312500 MtrunA17_Chr1g0184791 39.877 163 85 2 97 259 77 226 3.20e-30 113
Msa1312500 MtrunA17_Chr1g0187921 36.041 197 85 5 78 262 53 220 1.69e-29 111
Msa1312500 MtrunA17_Chr8g0390281 33.333 270 147 9 10 262 24 277 1.81e-29 112
Msa1312500 MtrunA17_Chr2g0331411 41.401 157 77 3 106 262 169 310 2.51e-29 113
Msa1312500 MtrunA17_Chr1g0190711 42.038 157 78 5 106 262 94 237 1.36e-28 109
Msa1312500 MtrunA17_Chr7g0260621 34.409 186 79 5 81 262 51 197 3.32e-27 104
Msa1312500 MtrunA17_Chr1g0166011 29.545 264 103 7 10 269 1 185 4.17e-27 103
Msa1312500 MtrunA17_Chr1g0187961 34.783 184 70 6 79 262 41 174 2.13e-26 101
Msa1312500 MtrunA17_Chr1g0184801 29.008 262 111 6 5 262 7 197 4.55e-26 101
Msa1312500 MtrunA17_Chr7g0260641 36.364 165 87 4 95 259 85 231 1.27e-23 96.3
Msa1312500 MtrunA17_Chr4g0004181 32.941 170 69 3 102 271 47 171 9.06e-20 84.3
Msa1312500 MtrunA17_Chr4g0069561 37.143 105 50 1 157 261 94 182 2.04e-18 80.9
Msa1312500 MtrunA17_Chr1g0193861 36.111 108 54 3 152 259 78 170 9.63e-18 78.6
Msa1312500 MtrunA17_Chr7g0270851 37.255 102 49 1 158 259 69 155 1.19e-17 78.2
Msa1312500 MtrunA17_Chr2g0332141 36.893 103 58 1 159 261 8 103 4.05e-17 75.5
Msa1312500 MtrunA17_Chr7g0239061 32.143 112 54 1 160 271 573 662 3.89e-14 72.0
Msa1312500 MtrunA17_Chr3g0137601 26.163 172 86 5 100 262 70 209 8.93e-14 68.6
Msa1312500 MtrunA17_Chr1g0209181 39.604 101 44 2 159 259 85 168 2.92e-13 66.6
Msa1312500 MtrunA17_Chr4g0064131 26.797 153 73 5 109 260 92 206 3.42e-13 67.0
Msa1312500 MtrunA17_Chr8g0346711 30.769 104 50 1 160 263 561 642 3.90e-11 63.2
Msa1312500 MtrunA17_Chr8g0385791 27.536 138 70 4 135 270 693 802 4.04e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1312500 AT3G16500.1 47.619 273 107 7 2 272 25 263 3.04e-68 213
Msa1312500 AT3G16500.2 48.289 263 101 6 2 263 25 253 5.79e-68 212
Msa1312500 AT1G51950.1 45.387 271 94 7 2 263 26 251 7.85e-62 196
Msa1312500 AT5G25890.1 43.605 172 57 3 101 272 40 171 1.27e-35 126
Msa1312500 AT2G33310.2 42.331 163 80 4 106 262 73 227 1.64e-32 120
Msa1312500 AT2G33310.3 42.593 162 79 4 107 262 73 226 2.13e-32 119
Msa1312500 AT2G33310.1 42.593 162 79 4 107 262 73 226 2.13e-32 119
Msa1312500 AT4G29080.1 38.953 172 93 3 96 262 125 289 4.83e-31 117
Msa1312500 AT2G22670.5 42.405 158 74 5 106 262 162 303 6.02e-31 117
Msa1312500 AT2G22670.2 42.405 158 74 5 106 262 162 303 6.02e-31 117
Msa1312500 AT2G22670.3 42.405 158 74 5 106 262 162 303 7.23e-31 117
Msa1312500 AT2G22670.1 42.405 158 74 5 106 262 162 303 7.23e-31 117
Msa1312500 AT2G22670.4 42.405 158 74 5 106 262 179 320 1.02e-30 117
Msa1312500 AT4G28640.1 38.217 157 85 2 110 262 89 237 1.07e-30 115
Msa1312500 AT4G28640.3 38.217 157 85 2 110 262 89 237 1.57e-30 115
Msa1312500 AT4G28640.2 39.490 157 83 2 110 262 89 237 5.23e-30 114
Msa1312500 AT1G04240.1 33.173 208 90 6 68 269 24 188 3.93e-29 109
Msa1312500 AT1G04240.2 33.173 208 90 6 68 269 124 288 2.67e-28 110
Msa1312500 AT5G65670.1 38.365 159 78 4 106 262 180 320 4.03e-28 110
Msa1312500 AT5G65670.2 38.365 159 78 4 106 262 180 320 4.32e-28 110
Msa1312500 AT1G04550.2 37.222 180 90 5 90 262 56 219 6.37e-28 107
Msa1312500 AT1G04100.1 38.509 161 88 3 111 261 95 254 8.98e-28 108
Msa1312500 AT1G04250.1 37.288 177 79 3 91 262 64 213 1.00e-27 107
Msa1312500 AT3G04730.1 34.529 223 137 5 42 262 5 220 1.42e-26 104
Msa1312500 AT5G43700.1 37.427 171 65 5 103 269 52 184 2.49e-26 102
Msa1312500 AT4G14550.1 37.580 157 81 2 106 262 73 212 4.05e-26 102
Msa1312500 AT4G14550.2 37.580 157 81 2 106 262 73 212 6.20e-26 102
Msa1312500 AT3G15540.1 37.349 166 59 5 106 268 67 190 8.39e-25 98.6
Msa1312500 AT3G23030.1 36.709 158 53 3 110 267 61 171 3.51e-24 96.3
Msa1312500 AT3G23050.1 34.132 167 81 3 106 262 79 226 5.58e-24 97.4
Msa1312500 AT3G23030.2 36.709 158 53 3 110 267 148 258 1.35e-23 97.1
Msa1312500 AT2G46990.1 41.284 109 48 2 153 261 80 172 1.65e-20 86.7
Msa1312500 AT1G52830.1 31.844 179 73 4 90 268 57 186 3.54e-20 85.9
Msa1312500 AT3G62100.1 40.741 108 48 2 153 260 78 169 3.74e-20 85.5
Msa1312500 AT4G14550.3 35.714 140 73 2 106 245 73 195 2.36e-19 84.0
Msa1312500 AT3G17600.1 32.927 164 67 6 96 259 34 154 4.56e-19 82.0
Msa1312500 AT4G14560.1 39.252 107 49 2 159 265 76 166 3.89e-18 80.1
Msa1312500 AT3G23050.2 31.788 151 74 3 106 246 79 210 4.78e-17 78.2
Msa1312500 AT1G15580.1 34.416 154 55 5 108 261 52 159 1.44e-16 75.5
Msa1312500 AT3G62100.2 39.785 93 40 2 153 245 78 154 2.20e-13 66.6
Msa1312500 AT2G46990.2 39.785 93 40 2 153 245 80 156 3.72e-13 66.2
Msa1312500 AT2G01200.2 33.333 108 55 2 155 262 96 186 8.13e-12 63.2
Msa1312500 AT2G01200.1 33.333 108 55 2 155 262 96 186 8.13e-12 63.2
Msa1312500 AT1G04550.1 34.513 113 61 3 90 195 56 162 9.71e-12 62.8
Msa1312500 AT1G15050.2 31.356 118 61 4 148 262 48 148 2.66e-11 60.8
Msa1312500 AT1G15050.1 31.356 118 61 4 148 262 80 180 3.65e-11 61.2
Msa1312500 AT4G14550.4 35.632 87 42 1 106 192 73 145 4.52e-11 60.8

Find 91 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGATAAACCTATCTTTA+AGG 0.110850 8_4:+9516540 None:intergenic
TTACATTCACACACAATATT+TGG 0.133960 8_4:+9513261 None:intergenic
ATCATTATTATAGGTTCTTC+TGG 0.198887 8_4:-9516524 Msa1312500:five_prime_UTR
TTGCATTCATACACATTATT+AGG 0.225646 8_4:+9513447 None:intergenic
TTTGGTTTCACACGCTTAAA+TGG 0.243122 8_4:+9516676 None:intergenic
TATAGCTTGGTCAACGATTC+AGG 0.244623 8_4:-9516856 Msa1312500:intron
CTTCATTAATGAAAATAAAA+AGG 0.250183 8_4:+9513238 None:intergenic
GATAGGTTTATCATTATTAT+AGG 0.254162 8_4:-9516533 Msa1312500:five_prime_UTR
ACTTGTTTCAATCTAGAAAA+AGG 0.257361 8_4:-9516458 Msa1312500:five_prime_UTR
CCTTTACCAGAGTTGCTAAT+AGG 0.291470 8_4:+9515648 None:intergenic
GAACAAATTCAAAGGAAATA+AGG 0.316814 8_4:+9516611 None:intergenic
AAGAAGCCACAAGTGTTCTT+TGG 0.323588 8_4:-9516389 Msa1312500:CDS
ATCAATGCAAGTGATGAAAA+AGG 0.328321 8_4:-9516218 Msa1312500:CDS
GGCCTCCGATTCGTTCATTC+AGG 0.338350 8_4:-9515758 Msa1312500:CDS
TAATCTACTTTAGCCCAAAA+AGG 0.354066 8_4:+9513490 None:intergenic
GAAATTCAGCTTTACCAAAC+AGG 0.360417 8_4:-9516120 Msa1312500:CDS
ATGATCAACGTTTACCTTCA+AGG 0.384045 8_4:+9515511 None:intergenic
AATATGGATGGAGTTCCTAT+TGG 0.394972 8_4:-9515609 Msa1312500:CDS
CTTCAAGGAGACCTCTAAAT+AGG 0.400379 8_4:+9515526 None:intergenic
GCTGTTGATGACCTATTTAG+AGG 0.418459 8_4:-9515537 Msa1312500:CDS
CTTGAGCTCAAGACTTGGAT+TGG 0.420807 8_4:-9513390 Msa1312500:three_prime_UTR
AAGCGTGTGAAACCAAAGTT+AGG 0.420992 8_4:-9516670 Msa1312500:five_prime_UTR
ATGGAATAGTTGCCTAACTT+TGG 0.430912 8_4:+9516658 None:intergenic
GAAGGAGACAAGATGCTTGT+AGG 0.431752 8_4:-9514775 Msa1312500:CDS
AAGCAAGAGGAAGAAGATAA+AGG 0.433944 8_4:-9514853 Msa1312500:CDS
AGCAGGAAGATCAGAACTCT+TGG 0.434060 8_4:+9514409 None:intergenic
TCATTATTATAGGTTCTTCT+GGG 0.438660 8_4:-9516523 Msa1312500:five_prime_UTR
CTATTTAGAGGTCTCCTTGA+AGG 0.443321 8_4:-9515525 Msa1312500:intron
TTATAGGTTCTTCTGGGAGG+TGG 0.456111 8_4:-9516517 Msa1312500:five_prime_UTR
AAAGGATCATTGACTGGAAG+TGG 0.456184 8_4:-9514826 Msa1312500:CDS
ATATGGATGGAGTTCCTATT+GGG 0.456891 8_4:-9515608 Msa1312500:CDS
TCATTAATGAAGATTTAAGT+TGG 0.456981 8_4:-9513227 Msa1312500:three_prime_UTR
GCTGAATTTCTTGCAGAAGA+AGG 0.458077 8_4:+9516133 None:intergenic
GCCTACTGAGTCCCGGCCTA+TGG 0.458539 8_4:-9515703 Msa1312500:CDS
CTATGATACAGATGTCTCTT+CGG 0.468482 8_4:+9517019 None:intergenic
CTTTGCATTGCCGAGTCAAG+TGG 0.471854 8_4:+9513573 None:intergenic
CTTGTTTGTGAAGATCAATA+TGG 0.475066 8_4:-9515625 Msa1312500:CDS
AAGGAGACAAGATGCTTGTA+GGG 0.476173 8_4:-9514774 Msa1312500:CDS
TTCTTTGGAGGAGAGGAAGT+TGG 0.476777 8_4:-9516374 Msa1312500:CDS
ATTGACATCAATGCTTATGA+TGG 0.483130 8_4:-9515579 Msa1312500:CDS
CTTGTTTATGAAGATAATGA+AGG 0.488627 8_4:-9514793 Msa1312500:CDS
CCTATTAGCAACTCTGGTAA+AGG 0.491874 8_4:-9515648 Msa1312500:CDS
TACCAAACAGGTCTCAGAAA+AGG 0.493666 8_4:-9516108 Msa1312500:intron
CATTGATTAAAGTTATAGCT+TGG 0.495869 8_4:-9516869 Msa1312500:five_prime_UTR
GAAATACTAAAACAATTAAA+CGG 0.499774 8_4:-9516948 Msa1312500:five_prime_UTR
GAAGAAGATAAAGGCATCAA+AGG 0.503044 8_4:-9514844 Msa1312500:CDS
GCAGAGATAAATTTATCTCA+TGG 0.505424 8_4:-9514886 Msa1312500:intron
ATCGGAGGCCAACCTACTAC+TGG 0.509519 8_4:+9515771 None:intergenic
GAGCTTAGACTTGGTCCACC+TGG 0.509843 8_4:-9516352 Msa1312500:CDS
ATGATATTTGATGCATCACT+AGG 0.521075 8_4:-9513302 Msa1312500:three_prime_UTR
AGGAAGGATGAGGCTGTTTG+AGG 0.522837 8_4:-9513552 Msa1312500:three_prime_UTR
GAAGAATCATCACTCACTCT+TGG 0.524837 8_4:-9516274 Msa1312500:CDS
GGCATCAAAGGATCATTGAC+TGG 0.528381 8_4:-9514832 Msa1312500:CDS
AAGCCACAAGTGTTCTTTGG+AGG 0.532124 8_4:-9516386 Msa1312500:CDS
TTCTTCCTGAATGAACGAAT+CGG 0.538928 8_4:+9515753 None:intergenic
AGGAAGTTGGAGCTTAGACT+TGG 0.541596 8_4:-9516361 Msa1312500:CDS
ACAAGTGTTCTTTGGAGGAG+AGG 0.544453 8_4:-9516381 Msa1312500:CDS
AATGCTGCTAGTCCAGTAGT+AGG 0.545474 8_4:-9515783 Msa1312500:CDS
CTGCTAGTCCAGTAGTAGGT+TGG 0.549015 8_4:-9515779 Msa1312500:CDS
TGCTTGTAGGGGACGTGCCG+TGG 0.551530 8_4:-9514762 Msa1312500:CDS
ATATCCGTGTGCTGAAAGCT+TGG 0.551850 8_4:-9513183 Msa1312500:three_prime_UTR
TTGACAAACTCTTCTTCACC+AGG 0.560561 8_4:+9516334 None:intergenic
AAATCCAAGCTTTCAGCACA+CGG 0.560697 8_4:+9513179 None:intergenic
CCTTGTCTTGAACCATAGGC+CGG 0.561981 8_4:+9515691 None:intergenic
CCGGCCTATGGTTCAAGACA+AGG 0.569410 8_4:-9515691 Msa1312500:CDS
TTCAGGAAGAACATTGCAAG+TGG 0.570187 8_4:-9515741 Msa1312500:CDS
CTATTCCATGCTATATTCTG+TGG 0.576490 8_4:-9516644 Msa1312500:five_prime_UTR
ATAACCTTGTCTTGAACCAT+AGG 0.576939 8_4:+9515687 None:intergenic
ATGTTCTTGAGCTCAAGACT+TGG 0.578024 8_4:-9513395 Msa1312500:three_prime_UTR
TCTTGCAAGTTTGTGCAACA+AGG 0.584527 8_4:+9516178 None:intergenic
CACTTGACTCGGCAATGCAA+AGG 0.589695 8_4:-9513572 Msa1312500:three_prime_UTR
ATACATTAGACCACTTGACT+CGG 0.590858 8_4:-9513583 Msa1312500:three_prime_UTR
TGGAATTAACAAGAAGCAAG+AGG 0.591043 8_4:-9514866 Msa1312500:CDS
GGCAATGCAAAGGAAGGATG+AGG 0.594384 8_4:-9513562 Msa1312500:three_prime_UTR
AGTGAACAGAACAAATTCAA+AGG 0.595215 8_4:+9516603 None:intergenic
CTAAGCCGCCTACTGAGTCC+CGG 0.597620 8_4:-9515710 Msa1312500:CDS
TGACTCGGCAATGCAAAGGA+AGG 0.598328 8_4:-9513568 Msa1312500:three_prime_UTR
CTTGTCTTGAACCATAGGCC+GGG 0.603934 8_4:+9515692 None:intergenic
TTTGTGAAGATCAATATGGA+TGG 0.610573 8_4:-9515621 Msa1312500:CDS
TATATATTCTACTAATGTAG+AGG 0.613994 8_4:-9513341 Msa1312500:three_prime_UTR
TGATAAGATACTTACTGCCA+CGG 0.622281 8_4:+9514745 None:intergenic
ATGAGCCACAGAATATAGCA+TGG 0.623301 8_4:+9516639 None:intergenic
TTATTATAGGTTCTTCTGGG+AGG 0.624893 8_4:-9516520 Msa1312500:five_prime_UTR
TGTTTGTATCAACAGTGAAG+AGG 0.629944 8_4:-9514442 Msa1312500:CDS
AAGACTTGGATTGGATGCTG+AGG 0.634139 8_4:-9513381 Msa1312500:three_prime_UTR
TCTCCTCCAAAGAACACTTG+TGG 0.635664 8_4:+9516383 None:intergenic
TGATATTTGATGCATCACTA+GGG 0.637267 8_4:-9513301 Msa1312500:three_prime_UTR
ATAGGCCGGGACTCAGTAGG+CGG 0.638385 8_4:+9515705 None:intergenic
TTCCTGAATGAACGAATCGG+AGG 0.657416 8_4:+9515756 None:intergenic
ACAAACTCTTCTTCACCAGG+TGG 0.675573 8_4:+9516337 None:intergenic
AGGAGACAAGATGCTTGTAG+GGG 0.714162 8_4:-9514773 Msa1312500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTTAATATGTTTGTT+TGG - chr8_4:9515731-9515750 Msa1312500:CDS 10.0%
!! GAAATACTAAAACAATTAAA+CGG - chr8_4:9513253-9513272 Msa1312500:three_prime_UTR 15.0%
!!! GTATATTTTTAATGATCTAA+CGG + chr8_4:9515189-9515208 None:intergenic 15.0%
!! TTAAAACAAAATTCACTTAA+AGG + chr8_4:9515248-9515267 None:intergenic 15.0%
!!! ACTATAGTTTTAAAAAACAA+AGG + chr8_4:9515577-9515596 None:intergenic 15.0%
!! AATGATTTAGTTATGATAAT+TGG - chr8_4:9515682-9515701 Msa1312500:CDS 15.0%
!! TACAAATTAAAGAGTAATTA+GGG - chr8_4:9515911-9515930 Msa1312500:intron 15.0%
!! CTTCATTAATGAAAATAAAA+AGG + chr8_4:9516966-9516985 None:intergenic 15.0%
!!! TTTCAATTACATTTTTGTCT+CGG + chr8_4:9513237-9513256 None:intergenic 20.0%
!! CAATGTTATAAAAACTGATT+TGG + chr8_4:9513317-9513336 None:intergenic 20.0%
!! TAATGATAAACCTATCTTTA+AGG + chr8_4:9513664-9513683 None:intergenic 20.0%
!! GATAGGTTTATCATTATTAT+AGG - chr8_4:9513668-9513687 Msa1312500:intron 20.0%
!! ATAAAGATGTAAATTGTTGT+TGG - chr8_4:9514213-9514232 Msa1312500:intron 20.0%
!!! TTCACCAAACAAATATTTTA+AGG - chr8_4:9515109-9515128 Msa1312500:intron 20.0%
!! TTGTCCTTAAAATATTTGTT+TGG + chr8_4:9515116-9515135 None:intergenic 20.0%
!!! AACAAAGGTTAATTTTACAT+TGG + chr8_4:9515562-9515581 None:intergenic 20.0%
!! AAAAACTCTTAGTTATTGTA+AGG + chr8_4:9515875-9515894 None:intergenic 20.0%
!! GTACAAATTAAAGAGTAATT+AGG - chr8_4:9515910-9515929 Msa1312500:intron 20.0%
!! AATTGCAAATGAAATTTATG+AGG - chr8_4:9516086-9516105 Msa1312500:intron 20.0%
!! GTAAAATTTATTTACGTGTT+TGG - chr8_4:9516108-9516127 Msa1312500:intron 20.0%
!! TTCAATAGTTCATAAAATTC+AGG - chr8_4:9516314-9516333 Msa1312500:CDS 20.0%
!! ATCACAACTAAAATAAATCA+GGG + chr8_4:9516544-9516563 None:intergenic 20.0%
!! TATATATTCTACTAATGTAG+AGG - chr8_4:9516860-9516879 Msa1312500:five_prime_UTR 20.0%
!! TCATTAATGAAGATTTAAGT+TGG - chr8_4:9516974-9516993 Msa1312500:five_prime_UTR 20.0%
!!! GTTATAAAAACTGATTTGGA+CGG + chr8_4:9513313-9513332 None:intergenic 25.0%
!! CATTGATTAAAGTTATAGCT+TGG - chr8_4:9513332-9513351 Msa1312500:three_prime_UTR 25.0%
! TAACAAACCAAAAACAGAAA+GGG + chr8_4:9513443-9513462 None:intergenic 25.0%
!! TCTTTTTCTTTCCTCTTTTT+AGG + chr8_4:9513504-9513523 None:intergenic 25.0%
! GAACAAATTCAAAGGAAATA+AGG + chr8_4:9513593-9513612 None:intergenic 25.0%
! ATCATTATTATAGGTTCTTC+TGG - chr8_4:9513677-9513696 Msa1312500:intron 25.0%
! TCATTATTATAGGTTCTTCT+GGG - chr8_4:9513678-9513697 Msa1312500:intron 25.0%
! ACTTGTTTCAATCTAGAAAA+AGG - chr8_4:9513743-9513762 Msa1312500:intron 25.0%
! GACACAATTATTGCATTAAA+AGG + chr8_4:9514851-9514870 None:intergenic 25.0%
!! TGGAAATGTTTTTCTTTGTT+GGG - chr8_4:9514933-9514952 Msa1312500:intron 25.0%
! TTTCAATCATAACTACTTCT+AGG - chr8_4:9515219-9515238 Msa1312500:intron 25.0%
! CTTGTTTATGAAGATAATGA+AGG - chr8_4:9515408-9515427 Msa1312500:intron 25.0%
! AAAACTCACACAATAACATA+GGG + chr8_4:9515650-9515669 None:intergenic 25.0%
!! TTAACAAGTACTTCTAAAGA+AGG - chr8_4:9515838-9515857 Msa1312500:intron 25.0%
!! TAACAAGTACTTCTAAAGAA+GGG - chr8_4:9515839-9515858 Msa1312500:intron 25.0%
! GATCACAACTAAAATAAATC+AGG + chr8_4:9516545-9516564 None:intergenic 25.0%
!!! AGTGTTTTTGTTTGACATTT+TGG - chr8_4:9516686-9516705 Msa1312500:five_prime_UTR 25.0%
! TTGCATTCATACACATTATT+AGG + chr8_4:9516757-9516776 None:intergenic 25.0%
! TTACATTCACACACAATATT+TGG + chr8_4:9516943-9516962 None:intergenic 25.0%
AAAAACAGAAAGGGAATTAG+TGG + chr8_4:9513434-9513453 None:intergenic 30.0%
! ACTAATTCCCTTTCTGTTTT+TGG - chr8_4:9513433-9513452 Msa1312500:three_prime_UTR 30.0%
CTAACAAACCAAAAACAGAA+AGG + chr8_4:9513444-9513463 None:intergenic 30.0%
!!! CTTTCTGTTTTTGGTTTGTT+AGG - chr8_4:9513442-9513461 Msa1312500:three_prime_UTR 30.0%
AGTGAACAGAACAAATTCAA+AGG + chr8_4:9513601-9513620 None:intergenic 30.0%
! CAAAGTTTTTCACTTCTTGA+TGG - chr8_4:9513630-9513649 Msa1312500:intron 30.0%
!! GGTTTTTAAGCCTTAAAGAT+AGG - chr8_4:9513651-9513670 Msa1312500:intron 30.0%
! TTTTTGTAAGCAACAAGATG+TGG - chr8_4:9513787-9513806 Msa1312500:intron 30.0%
ATCAATGCAAGTGATGAAAA+AGG - chr8_4:9513983-9514002 Msa1312500:intron 30.0%
GATATAAGAGAGAAAAGCAA+AGG + chr8_4:9514123-9514142 None:intergenic 30.0%
CTCTCTTATATCTATGTTTG+TGG - chr8_4:9514131-9514150 Msa1312500:intron 30.0%
AAAAAACCTATTAGCAACTC+TGG - chr8_4:9514547-9514566 Msa1312500:intron 30.0%
CTTGTTTGTGAAGATCAATA+TGG - chr8_4:9514576-9514595 Msa1312500:intron 30.0%
TTTGTGAAGATCAATATGGA+TGG - chr8_4:9514580-9514599 Msa1312500:intron 30.0%
! ATGTCAATTTTTCTCCCAAT+AGG + chr8_4:9514610-9514629 None:intergenic 30.0%
ATTGACATCAATGCTTATGA+TGG - chr8_4:9514622-9514641 Msa1312500:intron 30.0%
!! GTCTGATTTTAATCTATCGA+CGG - chr8_4:9514768-9514787 Msa1312500:CDS 30.0%
AATTATTGCATTAAAAGGCC+AGG + chr8_4:9514846-9514865 None:intergenic 30.0%
!! TGACATAACTTTTTGTTGTG+TGG - chr8_4:9514913-9514932 Msa1312500:intron 30.0%
! GTGGAAATGTTTTTCTTTGT+TGG - chr8_4:9514932-9514951 Msa1312500:intron 30.0%
CAAAAGATGTAGGTAACAAA+AGG + chr8_4:9514993-9515012 None:intergenic 30.0%
GCTATTTAGTCAAAAGATGT+AGG + chr8_4:9515003-9515022 None:intergenic 30.0%
!!! GTTTCAATTGCATTTTAGCT+AGG + chr8_4:9515061-9515080 None:intergenic 30.0%
GCAGAGATAAATTTATCTCA+TGG - chr8_4:9515315-9515334 Msa1312500:intron 30.0%
CAAAACTCACACAATAACAT+AGG + chr8_4:9515651-9515670 None:intergenic 30.0%
AAAAACTCACTGTTAAAAGC+AGG + chr8_4:9515812-9515831 None:intergenic 30.0%
! AACAAGTACTTCTAAAGAAG+GGG - chr8_4:9515840-9515859 Msa1312500:intron 30.0%
! TGACTAAGCGATCATAATTT+TGG + chr8_4:9515946-9515965 None:intergenic 30.0%
GCTTAGTCATAAATTGTAAC+AGG - chr8_4:9515957-9515976 Msa1312500:intron 30.0%
GTGACATGTTAATGAATATG+TGG - chr8_4:9516028-9516047 Msa1312500:intron 30.0%
! TTTAGTCCTTAATGACTTGT+GGG + chr8_4:9516068-9516087 None:intergenic 30.0%
ATTTAGTCCTTAATGACTTG+TGG + chr8_4:9516069-9516088 None:intergenic 30.0%
AAATATTGTGATGTAGAAGC+TGG + chr8_4:9516402-9516421 None:intergenic 30.0%
TAAATGTTCTTGTTCACAGT+TGG - chr8_4:9516575-9516594 Msa1312500:five_prime_UTR 30.0%
!!! TTGACATTTTGGTTCCTTTT+TGG - chr8_4:9516697-9516716 Msa1312500:five_prime_UTR 30.0%
!!! TGACATTTTGGTTCCTTTTT+GGG - chr8_4:9516698-9516717 Msa1312500:five_prime_UTR 30.0%
TAATCTACTTTAGCCCAAAA+AGG + chr8_4:9516714-9516733 None:intergenic 30.0%
!! TTTTTGGGCTAAAGTAGATT+AGG - chr8_4:9516713-9516732 Msa1312500:five_prime_UTR 30.0%
! TTTTGGGCTAAAGTAGATTA+GGG - chr8_4:9516714-9516733 Msa1312500:five_prime_UTR 30.0%
ATGATATTTGATGCATCACT+AGG - chr8_4:9516899-9516918 Msa1312500:five_prime_UTR 30.0%
TGATATTTGATGCATCACTA+GGG - chr8_4:9516900-9516919 Msa1312500:five_prime_UTR 30.0%
!! CATTTTTGTCTCGGTTTGAA+TGG + chr8_4:9513228-9513247 None:intergenic 35.0%
CGCAACTTGAAACAAATGTT+AGG + chr8_4:9513403-9513422 None:intergenic 35.0%
!!! GTTTTTGGTTTGTTAGGCTA+AGG - chr8_4:9513448-9513467 Msa1312500:three_prime_UTR 35.0%
TCAAACCTATGCCTAAAAAG+AGG - chr8_4:9513490-9513509 Msa1312500:three_prime_UTR 35.0%
!! TCTTTCCTCTTTTTAGGCAT+AGG + chr8_4:9513498-9513517 None:intergenic 35.0%
! TTTGGTTTCACACGCTTAAA+TGG + chr8_4:9513528-9513547 None:intergenic 35.0%
ATGGAATAGTTGCCTAACTT+TGG + chr8_4:9513546-9513565 None:intergenic 35.0%
CTATTCCATGCTATATTCTG+TGG - chr8_4:9513557-9513576 Msa1312500:three_prime_UTR 35.0%
TTATTATAGGTTCTTCTGGG+AGG - chr8_4:9513681-9513700 Msa1312500:intron 35.0%
GAAATTCAGCTTTACCAAAC+AGG - chr8_4:9514081-9514100 Msa1312500:intron 35.0%
!!! AGCAGCATTTCTGTTTTAGT+TGG + chr8_4:9514407-9514426 None:intergenic 35.0%
TTCTTCCTGAATGAACGAAT+CGG + chr8_4:9514451-9514470 None:intergenic 35.0%
ATAACCTTGTCTTGAACCAT+AGG + chr8_4:9514517-9514536 None:intergenic 35.0%
AATATGGATGGAGTTCCTAT+TGG - chr8_4:9514592-9514611 Msa1312500:intron 35.0%
ATATGGATGGAGTTCCTATT+GGG - chr8_4:9514593-9514612 Msa1312500:intron 35.0%
ATGATCAACGTTTACCTTCA+AGG + chr8_4:9514693-9514712 None:intergenic 35.0%
ATCTAACTCAATGTCTACCA+TGG - chr8_4:9514795-9514814 Msa1312500:CDS 35.0%
TGACTGTAGTAAAAATGCCA+TGG + chr8_4:9514815-9514834 None:intergenic 35.0%
CAAGTAACATTCAAAACTCC+TGG - chr8_4:9515022-9515041 Msa1312500:intron 35.0%
!! CTAGGTTGACATTTTATACC+AGG + chr8_4:9515043-9515062 None:intergenic 35.0%
ATGATTGATTGACAGTGCAA+CGG + chr8_4:9515164-9515183 None:intergenic 35.0%
CAATCATAACTACTTCTAGG+TGG - chr8_4:9515222-9515241 Msa1312500:intron 35.0%
TGGAATTAACAAGAAGCAAG+AGG - chr8_4:9515335-9515354 Msa1312500:intron 35.0%
AAGCAAGAGGAAGAAGATAA+AGG - chr8_4:9515348-9515367 Msa1312500:intron 35.0%
!! GAAGAAGATAAAGGCATCAA+AGG - chr8_4:9515357-9515376 Msa1312500:intron 35.0%
TGATAAGATACTTACTGCCA+CGG + chr8_4:9515459-9515478 None:intergenic 35.0%
CACACAATAACATAGGGAAA+AGG + chr8_4:9515644-9515663 None:intergenic 35.0%
!! AGCTATGATTTGTTACTGTG+TGG - chr8_4:9515706-9515725 Msa1312500:CDS 35.0%
TGTTTGTATCAACAGTGAAG+AGG - chr8_4:9515759-9515778 Msa1312500:CDS 35.0%
GGTCTGTCTAGTAACTTATT+TGG - chr8_4:9515991-9516010 Msa1312500:intron 35.0%
ATACATTAGACCACTTGACT+CGG - chr8_4:9516618-9516637 Msa1312500:five_prime_UTR 35.0%
TATAGCTTGGTCAACGATTC+AGG - chr8_4:9513345-9513364 Msa1312500:three_prime_UTR 40.0%
AAGCGTGTGAAACCAAAGTT+AGG - chr8_4:9513531-9513550 Msa1312500:three_prime_UTR 40.0%
ATGAGCCACAGAATATAGCA+TGG + chr8_4:9513565-9513584 None:intergenic 40.0%
AAGAAGCCACAAGTGTTCTT+TGG - chr8_4:9513812-9513831 Msa1312500:intron 40.0%
TTGACAAACTCTTCTTCACC+AGG + chr8_4:9513870-9513889 None:intergenic 40.0%
GAAGAATCATCACTCACTCT+TGG - chr8_4:9513927-9513946 Msa1312500:intron 40.0%
TCTTGCAAGTTTGTGCAACA+AGG + chr8_4:9514026-9514045 None:intergenic 40.0%
GCTGAATTTCTTGCAGAAGA+AGG + chr8_4:9514071-9514090 None:intergenic 40.0%
TACCAAACAGGTCTCAGAAA+AGG - chr8_4:9514093-9514112 Msa1312500:intron 40.0%
!!! TACCTTTTCTGAGACCTGTT+TGG + chr8_4:9514098-9514117 None:intergenic 40.0%
ATACCAAAACCGTAACGTTG+TGG - chr8_4:9514273-9514292 Msa1312500:intron 40.0%
CGTATCTGCAACCACAATTA+CGG + chr8_4:9514299-9514318 None:intergenic 40.0%
TTCAGGAAGAACATTGCAAG+TGG - chr8_4:9514460-9514479 Msa1312500:intron 40.0%
CCTTTACCAGAGTTGCTAAT+AGG + chr8_4:9514556-9514575 None:intergenic 40.0%
CCTATTAGCAACTCTGGTAA+AGG - chr8_4:9514553-9514572 Msa1312500:intron 40.0%
!! GCTGTTGATGACCTATTTAG+AGG - chr8_4:9514664-9514683 Msa1312500:intron 40.0%
CTTCAAGGAGACCTCTAAAT+AGG + chr8_4:9514678-9514697 None:intergenic 40.0%
CTATTTAGAGGTCTCCTTGA+AGG - chr8_4:9514676-9514695 Msa1312500:intron 40.0%
! AAAGGATCATTGACTGGAAG+TGG - chr8_4:9515375-9515394 Msa1312500:intron 40.0%
AAGGAGACAAGATGCTTGTA+GGG - chr8_4:9515427-9515446 Msa1312500:intron 40.0%
TTGTAACAGGTCAGATCAAG+TGG - chr8_4:9515970-9515989 Msa1312500:intron 40.0%
GTCAAACAAAAACACTCGTG+TGG + chr8_4:9516682-9516701 None:intergenic 40.0%
ATGTTCTTGAGCTCAAGACT+TGG - chr8_4:9516806-9516825 Msa1312500:intron 40.0%
! CTTGGTCAACGATTCAGGTT+AGG - chr8_4:9513350-9513369 Msa1312500:three_prime_UTR 45.0%
! TTATAGGTTCTTCTGGGAGG+TGG - chr8_4:9513684-9513703 Msa1312500:intron 45.0%
AAGCCACAAGTGTTCTTTGG+AGG - chr8_4:9513815-9513834 Msa1312500:intron 45.0%
TCTCCTCCAAAGAACACTTG+TGG + chr8_4:9513821-9513840 None:intergenic 45.0%
! ACAAGTGTTCTTTGGAGGAG+AGG - chr8_4:9513820-9513839 Msa1312500:intron 45.0%
! TTCTTTGGAGGAGAGGAAGT+TGG - chr8_4:9513827-9513846 Msa1312500:intron 45.0%
AGGAAGTTGGAGCTTAGACT+TGG - chr8_4:9513840-9513859 Msa1312500:intron 45.0%
ACAAACTCTTCTTCACCAGG+TGG + chr8_4:9513867-9513886 None:intergenic 45.0%
CAAGAACAACATTCACTCCG+CGG + chr8_4:9514186-9514205 None:intergenic 45.0%
CAATTACGGCCACAACGTTA+CGG + chr8_4:9514285-9514304 None:intergenic 45.0%
TAACGTTGTGGCCGTAATTG+TGG - chr8_4:9514285-9514304 Msa1312500:intron 45.0%
AATGCTGCTAGTCCAGTAGT+AGG - chr8_4:9514418-9514437 Msa1312500:CDS 45.0%
TTCCTGAATGAACGAATCGG+AGG + chr8_4:9514448-9514467 None:intergenic 45.0%
ACTACAGTCAATCCAGATCC+TGG - chr8_4:9514825-9514844 Msa1312500:CDS 45.0%
TGCATTAAAAGGCCAGGATC+TGG + chr8_4:9514840-9514859 None:intergenic 45.0%
GGCATCAAAGGATCATTGAC+TGG - chr8_4:9515369-9515388 Msa1312500:intron 45.0%
GAAGGAGACAAGATGCTTGT+AGG - chr8_4:9515426-9515445 Msa1312500:intron 45.0%
AGGAGACAAGATGCTTGTAG+GGG - chr8_4:9515428-9515447 Msa1312500:intron 45.0%
AGCAGGAAGATCAGAACTCT+TGG + chr8_4:9515795-9515814 None:intergenic 45.0%
ACTTGTGGGATGCACATATG+AGG + chr8_4:9516054-9516073 None:intergenic 45.0%
GTGCATCCCACAAGTCATTA+AGG - chr8_4:9516059-9516078 Msa1312500:intron 45.0%
CTTGAGCTCAAGACTTGGAT+TGG - chr8_4:9516811-9516830 Msa1312500:intron 45.0%
! AAGACTTGGATTGGATGCTG+AGG - chr8_4:9516820-9516839 Msa1312500:intron 45.0%
CTGCTAGTCCAGTAGTAGGT+TGG - chr8_4:9514422-9514441 Msa1312500:CDS 50.0%
CTTGTCTTGAACCATAGGCC+GGG + chr8_4:9514512-9514531 None:intergenic 50.0%
CCTTGTCTTGAACCATAGGC+CGG + chr8_4:9514513-9514532 None:intergenic 50.0%
CTTTGCATTGCCGAGTCAAG+TGG + chr8_4:9516631-9516650 None:intergenic 50.0%
!! CACTTGACTCGGCAATGCAA+AGG - chr8_4:9516629-9516648 Msa1312500:five_prime_UTR 50.0%
! TGACTCGGCAATGCAAAGGA+AGG - chr8_4:9516633-9516652 Msa1312500:five_prime_UTR 50.0%
GGCAATGCAAAGGAAGGATG+AGG - chr8_4:9516639-9516658 Msa1312500:five_prime_UTR 50.0%
AGGAAGGATGAGGCTGTTTG+AGG - chr8_4:9516649-9516668 Msa1312500:five_prime_UTR 50.0%
! GAGCTTAGACTTGGTCCACC+TGG - chr8_4:9513849-9513868 Msa1312500:intron 55.0%
GTCTGCAACTGCATCTACCG+CGG - chr8_4:9514166-9514185 Msa1312500:intron 55.0%
! CGGCCACAACGTTACGGTTT+TGG + chr8_4:9514279-9514298 None:intergenic 55.0%
ATCGGAGGCCAACCTACTAC+TGG + chr8_4:9514433-9514452 None:intergenic 55.0%
! GGCCTCCGATTCGTTCATTC+AGG - chr8_4:9514443-9514462 Msa1312500:CDS 55.0%
CCGGCCTATGGTTCAAGACA+AGG - chr8_4:9514510-9514529 Msa1312500:intron 55.0%
CTAAGCCGCCTACTGAGTCC+CGG - chr8_4:9514491-9514510 Msa1312500:intron 60.0%
ATAGGCCGGGACTCAGTAGG+CGG + chr8_4:9514499-9514518 None:intergenic 60.0%
ACCATAGGCCGGGACTCAGT+AGG + chr8_4:9514502-9514521 None:intergenic 60.0%
GCCTACTGAGTCCCGGCCTA+TGG - chr8_4:9514498-9514517 Msa1312500:intron 65.0%
TGCTTGTAGGGGACGTGCCG+TGG - chr8_4:9515439-9515458 Msa1312500:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 9513183 9517040 9513183 ID=Msa1312500;Name=Msa1312500
chr8_4 mRNA 9513183 9517040 9513183 ID=Msa1312500-mRNA-1;Parent=Msa1312500;Name=Msa1312500-mRNA-1;_AED=0.04;_eAED=0.04;_QI=524|1|1|1|0.8|0.66|6|427|273
chr8_4 exon 9513183 9513629 9513183 ID=Msa1312500-mRNA-1:exon:3520;Parent=Msa1312500-mRNA-1
chr8_4 exon 9514402 9514466 9514402 ID=Msa1312500-mRNA-1:exon:3519;Parent=Msa1312500-mRNA-1
chr8_4 exon 9514760 9514904 9514760 ID=Msa1312500-mRNA-1:exon:3518;Parent=Msa1312500-mRNA-1
chr8_4 exon 9515526 9515806 9515526 ID=Msa1312500-mRNA-1:exon:3517;Parent=Msa1312500-mRNA-1
chr8_4 exon 9516109 9516759 9516109 ID=Msa1312500-mRNA-1:exon:3516;Parent=Msa1312500-mRNA-1
chr8_4 exon 9516857 9517040 9516857 ID=Msa1312500-mRNA-1:exon:3515;Parent=Msa1312500-mRNA-1
chr8_4 five_prime_UTR 9516857 9517040 9516857 ID=Msa1312500-mRNA-1:five_prime_utr;Parent=Msa1312500-mRNA-1
chr8_4 five_prime_UTR 9516420 9516759 9516420 ID=Msa1312500-mRNA-1:five_prime_utr;Parent=Msa1312500-mRNA-1
chr8_4 CDS 9516109 9516419 9516109 ID=Msa1312500-mRNA-1:cds;Parent=Msa1312500-mRNA-1
chr8_4 CDS 9515526 9515806 9515526 ID=Msa1312500-mRNA-1:cds;Parent=Msa1312500-mRNA-1
chr8_4 CDS 9514760 9514904 9514760 ID=Msa1312500-mRNA-1:cds;Parent=Msa1312500-mRNA-1
chr8_4 CDS 9514402 9514466 9514402 ID=Msa1312500-mRNA-1:cds;Parent=Msa1312500-mRNA-1
chr8_4 CDS 9513610 9513629 9513610 ID=Msa1312500-mRNA-1:cds;Parent=Msa1312500-mRNA-1
chr8_4 three_prime_UTR 9513183 9513609 9513183 ID=Msa1312500-mRNA-1:three_prime_utr;Parent=Msa1312500-mRNA-1
Gene Sequence

>Msa1312500

ATGTGGCAAAGAAGCCACAAGTGTTCTTTGGAGGAGAGGAAGTTGGAGCTTAGACTTGGTCCACCTGGTGAAGAAGAGTTTGTCAATGAAAAAAGCTTGAAGAATGTCAAAAAAGAAAGAGATGAAGAATCATCACTCACTCTTGGTTATGCTAAAGCTTCATCATTTCTTAAGCTCTCATCAATGCAAGTGATGAAAAAGGATGCATCACAATCACAACCTTGTTGCACAAACTTGCAAGAAAGTGAGAAAAATGCATTCTCTCCTTCTTCTGCAAGAAATTCAGCTTTACCAAACAGGTCTCAGAAAAGAAATGCTGCTAGTCCAGTAGTAGGTTGGCCTCCGATTCGTTCATTCAGGAAGAACATTGCAAGTGGAAGCTCTTCTAAGCCGCCTACTGAGTCCCGGCCTATGGTTCAAGACAAGGTTATTGTTGATAGTAAAAAACCTATTAGCAACTCTGGTAAAGGCTTGTTTGTGAAGATCAATATGGATGGAGTTCCTATTGGGAGAAAAATTGACATCAATGCTTATGATGGCTATGAAAAACTTTCATCTGCTGTTGATGACCTATTTAGAGGTCTCCTTGAAGAGATAAATTTATCTCATGGAATTAACAAGAAGCAAGAGGAAGAAGATAAAGGCATCAAAGGATCATTGACTGGAAGTGGAGAATATACTCTTGTTTATGAAGATAATGAAGGAGACAAGATGCTTGTAGGGGACGTGCCGTGGCACATGTTTGTATCAACAGTGAAGAGGCTGCGTGTGTCCAAGAGTTCTGATCTTCCTGCTTTTAACATTGGTAGCAAGAAAGACTAG
Protein sequence

>Msa1312500

MWQRSHKCSLEERKLELRLGPPGEEEFVNEKSLKNVKKERDEESSLTLGYAKASSFLKLSSMQVMKKDASQSQPCCTNLQESEKNAFSPSSARNSALPNRSQKRNAASPVVGWPPIRSFRKNIASGSSSKPPTESRPMVQDKVIVDSKKPISNSGKGLFVKINMDGVPIGRKIDINAYDGYEKLSSAVDDLFRGLLEEINLSHGINKKQEEEDKGIKGSLTGSGEYTLVYEDNEGDKMLVGDVPWHMFVSTVKRLRVSKSSDLPAFNIGSKKD*