Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1180670 | KEH18217.1 | 96.667 | 270 | 7 | 1 | 1 | 270 | 1 | 268 | 0.0 | 531 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1180670 | sp|Q8LAL2|IAA26_ARATH | 47.925 | 265 | 99 | 6 | 2 | 263 | 25 | 253 | 2.91e-67 | 213 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa1180670 | TR | AUX/IAA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0136550 | Msa1180670 | 0.802057 | 6.721202e-49 | -8.615850e-47 |
| Msa0430060 | Msa1180670 | 0.803982 | 2.688331e-49 | -8.615850e-47 |
| Msa0430150 | Msa1180670 | 0.801072 | 1.069982e-48 | -8.615850e-47 |
| Msa1026640 | Msa1180670 | 0.802765 | 4.803128e-49 | -8.615850e-47 |
| Msa0308440 | Msa1180670 | 0.807113 | 5.922148e-50 | -8.615850e-47 |
| Msa1180670 | Msa1231810 | 0.818380 | 2.020359e-52 | -8.615850e-47 |
| Msa1180670 | Msa1236440 | 0.804216 | 2.402698e-49 | -8.615850e-47 |
| Msa1180670 | Msa1274510 | 0.902831 | 6.481458e-79 | -8.615850e-47 |
| Msa1180670 | Msa1312500 | 0.820763 | 5.777274e-53 | -8.615850e-47 |
| Msa1180670 | Msa1437330 | 0.804920 | 1.713764e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1180670 | MtrunA17_Chr8g0340731 | 97.004 | 267 | 6 | 1 | 1 | 267 | 1 | 265 | 0.0 | 528 |
| Msa1180670 | MtrunA17_Chr2g0330951 | 55.720 | 271 | 94 | 8 | 4 | 266 | 6 | 258 | 2.58e-82 | 248 |
| Msa1180670 | MtrunA17_Chr5g0411501 | 38.916 | 203 | 101 | 6 | 60 | 262 | 140 | 319 | 3.27e-33 | 123 |
| Msa1180670 | MtrunA17_Chr8g0366881 | 39.773 | 176 | 88 | 3 | 88 | 262 | 174 | 332 | 1.38e-32 | 122 |
| Msa1180670 | MtrunA17_Chr6g0486121 | 33.835 | 266 | 134 | 7 | 3 | 262 | 9 | 238 | 1.87e-32 | 119 |
| Msa1180670 | MtrunA17_Chr1g0183251 | 39.548 | 177 | 86 | 5 | 105 | 262 | 78 | 252 | 2.66e-31 | 117 |
| Msa1180670 | MtrunA17_Chr5g0428141 | 44.444 | 153 | 67 | 5 | 110 | 262 | 197 | 331 | 3.06e-30 | 115 |
| Msa1180670 | MtrunA17_Chr1g0184791 | 39.877 | 163 | 85 | 2 | 97 | 259 | 77 | 226 | 5.59e-30 | 112 |
| Msa1180670 | MtrunA17_Chr1g0187921 | 36.216 | 185 | 78 | 4 | 90 | 262 | 64 | 220 | 2.86e-29 | 110 |
| Msa1180670 | MtrunA17_Chr2g0331411 | 41.401 | 157 | 77 | 3 | 106 | 262 | 169 | 310 | 2.89e-29 | 112 |
| Msa1180670 | MtrunA17_Chr1g0190711 | 41.772 | 158 | 77 | 5 | 106 | 262 | 94 | 237 | 8.59e-29 | 110 |
| Msa1180670 | MtrunA17_Chr8g0390281 | 36.548 | 197 | 95 | 6 | 76 | 262 | 101 | 277 | 1.12e-28 | 110 |
| Msa1180670 | MtrunA17_Chr7g0260621 | 34.946 | 186 | 76 | 6 | 83 | 262 | 51 | 197 | 6.75e-27 | 103 |
| Msa1180670 | MtrunA17_Chr1g0166011 | 37.580 | 157 | 56 | 4 | 106 | 262 | 64 | 178 | 1.27e-26 | 102 |
| Msa1180670 | MtrunA17_Chr1g0187961 | 37.037 | 162 | 56 | 5 | 105 | 262 | 55 | 174 | 3.63e-26 | 101 |
| Msa1180670 | MtrunA17_Chr1g0184801 | 31.298 | 262 | 105 | 9 | 5 | 262 | 7 | 197 | 6.54e-26 | 101 |
| Msa1180670 | MtrunA17_Chr7g0260641 | 36.364 | 165 | 87 | 4 | 95 | 259 | 85 | 231 | 1.73e-23 | 95.9 |
| Msa1180670 | MtrunA17_Chr4g0004181 | 33.133 | 166 | 66 | 3 | 102 | 267 | 47 | 167 | 2.20e-19 | 83.2 |
| Msa1180670 | MtrunA17_Chr4g0069561 | 37.500 | 104 | 49 | 1 | 157 | 260 | 94 | 181 | 2.43e-18 | 80.5 |
| Msa1180670 | MtrunA17_Chr1g0193861 | 36.111 | 108 | 54 | 3 | 152 | 259 | 78 | 170 | 1.06e-17 | 78.6 |
| Msa1180670 | MtrunA17_Chr7g0270851 | 37.255 | 102 | 49 | 1 | 158 | 259 | 69 | 155 | 1.17e-17 | 78.2 |
| Msa1180670 | MtrunA17_Chr2g0332141 | 36.893 | 103 | 58 | 1 | 159 | 261 | 8 | 103 | 4.24e-17 | 75.5 |
| Msa1180670 | MtrunA17_Chr3g0137601 | 26.744 | 172 | 85 | 4 | 100 | 262 | 70 | 209 | 2.95e-14 | 70.1 |
| Msa1180670 | MtrunA17_Chr1g0209181 | 39.604 | 101 | 44 | 2 | 159 | 259 | 85 | 168 | 2.47e-13 | 66.6 |
| Msa1180670 | MtrunA17_Chr7g0239061 | 32.692 | 104 | 48 | 1 | 160 | 263 | 573 | 654 | 2.85e-13 | 69.7 |
| Msa1180670 | MtrunA17_Chr4g0064131 | 26.797 | 153 | 73 | 5 | 109 | 260 | 92 | 206 | 4.21e-13 | 66.6 |
| Msa1180670 | MtrunA17_Chr8g0346711 | 30.769 | 104 | 50 | 1 | 160 | 263 | 561 | 642 | 4.70e-11 | 62.8 |
| Msa1180670 | MtrunA17_Chr8g0385791 | 29.600 | 125 | 58 | 4 | 135 | 257 | 693 | 789 | 4.77e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1180670 | AT3G16500.1 | 47.925 | 265 | 99 | 6 | 2 | 263 | 25 | 253 | 2.96e-68 | 213 |
| Msa1180670 | AT3G16500.2 | 47.925 | 265 | 99 | 6 | 2 | 263 | 25 | 253 | 4.95e-68 | 212 |
| Msa1180670 | AT1G51950.1 | 45.620 | 274 | 89 | 9 | 2 | 263 | 26 | 251 | 1.02e-61 | 196 |
| Msa1180670 | AT5G25890.1 | 44.099 | 161 | 52 | 2 | 101 | 261 | 40 | 162 | 1.02e-34 | 123 |
| Msa1180670 | AT2G33310.3 | 40.609 | 197 | 93 | 6 | 82 | 269 | 55 | 236 | 3.47e-33 | 121 |
| Msa1180670 | AT2G33310.1 | 40.609 | 197 | 93 | 6 | 82 | 269 | 55 | 236 | 3.47e-33 | 121 |
| Msa1180670 | AT2G33310.2 | 40.609 | 197 | 94 | 6 | 82 | 269 | 55 | 237 | 8.55e-33 | 120 |
| Msa1180670 | AT4G29080.1 | 34.420 | 276 | 146 | 8 | 4 | 262 | 32 | 289 | 1.96e-32 | 121 |
| Msa1180670 | AT4G28640.1 | 40.506 | 158 | 80 | 4 | 110 | 262 | 89 | 237 | 4.35e-31 | 116 |
| Msa1180670 | AT4G28640.3 | 40.506 | 158 | 80 | 4 | 110 | 262 | 89 | 237 | 6.76e-31 | 115 |
| Msa1180670 | AT2G22670.5 | 44.025 | 159 | 70 | 7 | 106 | 262 | 162 | 303 | 1.73e-30 | 116 |
| Msa1180670 | AT2G22670.2 | 44.025 | 159 | 70 | 7 | 106 | 262 | 162 | 303 | 1.73e-30 | 116 |
| Msa1180670 | AT2G22670.3 | 44.025 | 159 | 70 | 7 | 106 | 262 | 162 | 303 | 1.84e-30 | 116 |
| Msa1180670 | AT2G22670.1 | 44.025 | 159 | 70 | 7 | 106 | 262 | 162 | 303 | 1.84e-30 | 116 |
| Msa1180670 | AT4G28640.2 | 41.772 | 158 | 78 | 4 | 110 | 262 | 89 | 237 | 1.88e-30 | 115 |
| Msa1180670 | AT2G22670.4 | 44.025 | 159 | 70 | 7 | 106 | 262 | 179 | 320 | 2.68e-30 | 116 |
| Msa1180670 | AT1G04240.1 | 34.328 | 201 | 83 | 6 | 68 | 262 | 24 | 181 | 2.56e-28 | 107 |
| Msa1180670 | AT1G04550.2 | 37.222 | 180 | 90 | 5 | 90 | 262 | 56 | 219 | 4.46e-28 | 108 |
| Msa1180670 | AT5G65670.2 | 38.608 | 158 | 79 | 4 | 106 | 262 | 180 | 320 | 5.35e-28 | 110 |
| Msa1180670 | AT5G65670.1 | 38.608 | 158 | 79 | 4 | 106 | 262 | 180 | 320 | 5.70e-28 | 110 |
| Msa1180670 | AT1G04100.1 | 39.752 | 161 | 86 | 3 | 111 | 261 | 95 | 254 | 1.26e-27 | 107 |
| Msa1180670 | AT1G04240.2 | 34.328 | 201 | 83 | 6 | 68 | 262 | 124 | 281 | 1.79e-27 | 107 |
| Msa1180670 | AT1G04250.1 | 37.288 | 177 | 79 | 3 | 91 | 262 | 64 | 213 | 1.87e-27 | 106 |
| Msa1180670 | AT3G04730.1 | 41.509 | 159 | 86 | 4 | 106 | 262 | 67 | 220 | 3.55e-26 | 103 |
| Msa1180670 | AT4G14550.1 | 37.580 | 157 | 81 | 2 | 106 | 262 | 73 | 212 | 4.52e-26 | 102 |
| Msa1180670 | AT4G14550.2 | 37.580 | 157 | 81 | 2 | 106 | 262 | 73 | 212 | 5.55e-26 | 102 |
| Msa1180670 | AT5G43700.1 | 33.161 | 193 | 80 | 5 | 82 | 263 | 24 | 178 | 2.22e-25 | 99.8 |
| Msa1180670 | AT3G15540.1 | 37.576 | 165 | 58 | 5 | 106 | 267 | 67 | 189 | 1.59e-24 | 97.8 |
| Msa1180670 | AT3G23030.1 | 36.709 | 158 | 53 | 3 | 110 | 267 | 61 | 171 | 4.01e-24 | 95.9 |
| Msa1180670 | AT3G23050.1 | 34.132 | 167 | 81 | 3 | 106 | 262 | 79 | 226 | 6.30e-24 | 97.4 |
| Msa1180670 | AT3G23030.2 | 36.709 | 158 | 53 | 3 | 110 | 267 | 148 | 258 | 1.63e-23 | 96.7 |
| Msa1180670 | AT2G46990.1 | 41.284 | 109 | 48 | 2 | 153 | 261 | 80 | 172 | 1.78e-20 | 86.3 |
| Msa1180670 | AT3G62100.1 | 40.741 | 108 | 48 | 2 | 153 | 260 | 78 | 169 | 3.69e-20 | 85.5 |
| Msa1180670 | AT1G52830.1 | 31.720 | 186 | 78 | 4 | 81 | 266 | 48 | 184 | 6.03e-20 | 85.5 |
| Msa1180670 | AT4G14550.3 | 35.714 | 140 | 73 | 2 | 106 | 245 | 73 | 195 | 2.33e-19 | 84.0 |
| Msa1180670 | AT3G17600.1 | 32.927 | 164 | 67 | 6 | 96 | 259 | 34 | 154 | 5.32e-19 | 82.0 |
| Msa1180670 | AT3G23050.2 | 31.788 | 151 | 74 | 3 | 106 | 246 | 79 | 210 | 5.30e-17 | 77.8 |
| Msa1180670 | AT4G14560.1 | 33.750 | 160 | 61 | 3 | 106 | 265 | 52 | 166 | 1.03e-16 | 76.3 |
| Msa1180670 | AT1G15580.1 | 34.416 | 154 | 55 | 5 | 108 | 261 | 52 | 159 | 1.63e-16 | 75.5 |
| Msa1180670 | AT3G62100.2 | 39.785 | 93 | 40 | 2 | 153 | 245 | 78 | 154 | 2.23e-13 | 66.6 |
| Msa1180670 | AT2G46990.2 | 39.785 | 93 | 40 | 2 | 153 | 245 | 80 | 156 | 3.73e-13 | 66.2 |
| Msa1180670 | AT1G04550.1 | 34.513 | 113 | 61 | 3 | 90 | 195 | 56 | 162 | 7.32e-12 | 62.8 |
| Msa1180670 | AT2G01200.2 | 33.333 | 108 | 55 | 2 | 155 | 262 | 96 | 186 | 8.68e-12 | 63.2 |
| Msa1180670 | AT2G01200.1 | 33.333 | 108 | 55 | 2 | 155 | 262 | 96 | 186 | 8.68e-12 | 63.2 |
| Msa1180670 | AT4G32280.1 | 26.901 | 171 | 83 | 5 | 108 | 259 | 93 | 240 | 1.46e-11 | 63.5 |
| Msa1180670 | AT1G15050.2 | 31.731 | 104 | 54 | 3 | 159 | 262 | 62 | 148 | 4.03e-11 | 60.5 |
| Msa1180670 | AT4G14550.4 | 35.632 | 87 | 42 | 1 | 106 | 192 | 73 | 145 | 4.13e-11 | 60.8 |
| Msa1180670 | AT1G15050.1 | 31.731 | 104 | 54 | 3 | 159 | 262 | 94 | 180 | 5.40e-11 | 60.8 |
| Msa1180670 | AT4G32280.2 | 26.708 | 161 | 82 | 3 | 114 | 259 | 59 | 198 | 8.26e-11 | 60.5 |
Find 75 sgRNAs with CRISPR-Local
Find 135 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATAATAAACCTTTCTTTA+AGG | 0.093197 | 8_1:-14873450 | None:intergenic |
| GAAAGGTTTATTATCATTAT+AGG | 0.206567 | 8_1:+14873457 | Msa1180670:five_prime_UTR |
| ATTATCATTATAGGTTCTTC+TGG | 0.226106 | 8_1:+14873466 | Msa1180670:five_prime_UTR |
| TTTGGTTTCACACGCTTAAA+TGG | 0.243122 | 8_1:-14873314 | None:intergenic |
| TATAGCTTGGTCAACGATTC+AGG | 0.244623 | 8_1:+14873134 | Msa1180670:five_prime_UTR |
| AATTGTTTCAATCTAGAAAA+AGG | 0.271380 | 8_1:+14873531 | Msa1180670:five_prime_UTR |
| CCTTTACCAGAGTTGCTAAT+AGG | 0.291470 | 8_1:-14874341 | None:intergenic |
| TAACAAATTCAAAGTAAATA+AGG | 0.317224 | 8_1:-14873379 | None:intergenic |
| AAGAAGCCACAAGTGTTCTT+TGG | 0.323588 | 8_1:+14873600 | Msa1180670:CDS |
| ATCAATGCAAGTGATGAAAA+AGG | 0.328321 | 8_1:+14873771 | Msa1180670:CDS |
| GGCCTCCGATTCGTTCATTC+AGG | 0.338350 | 8_1:+14874231 | Msa1180670:CDS |
| GAAATTCAGCTTTACCAAAC+AGG | 0.360417 | 8_1:+14873869 | Msa1180670:CDS |
| ATTATCAACGTTTACCTTCA+AGG | 0.362322 | 8_1:-14874478 | None:intergenic |
| AATATGGATGGAGTTCCTAT+TGG | 0.394972 | 8_1:+14874380 | Msa1180670:CDS |
| CTTCAAGGAGACCTCTAAAT+AGG | 0.400379 | 8_1:-14874463 | None:intergenic |
| GCTGTTGATGACCTATTTAG+AGG | 0.418459 | 8_1:+14874452 | Msa1180670:CDS |
| AAGCGTGTGAAACCAAAGTT+AGG | 0.420992 | 8_1:+14873320 | Msa1180670:five_prime_UTR |
| ATGGAATAGTTGCCTAACTT+TGG | 0.430912 | 8_1:-14873332 | None:intergenic |
| GAAGGAGACAAGATGCTTGT+AGG | 0.431752 | 8_1:+14875212 | Msa1180670:CDS |
| AAGCAAGAGGAAGAAGATAA+AGG | 0.433944 | 8_1:+14875134 | Msa1180670:CDS |
| AGCAGGAAGATCAGAACTCT+TGG | 0.434060 | 8_1:-14875574 | None:intergenic |
| TTATCATTATAGGTTCTTCT+GGG | 0.438660 | 8_1:+14873467 | Msa1180670:five_prime_UTR |
| CTATTTAGAGGTCTCCTTGA+AGG | 0.443321 | 8_1:+14874464 | Msa1180670:CDS |
| TTATAGGTTCTTCTGGGAGG+TGG | 0.450924 | 8_1:+14873473 | Msa1180670:five_prime_UTR |
| AAAGGATCATTGACTGGAAG+TGG | 0.456184 | 8_1:+14875161 | Msa1180670:CDS |
| ATATGGATGGAGTTCCTATT+GGG | 0.456891 | 8_1:+14874381 | Msa1180670:CDS |
| GCTGAATTTCTTGCAGAAGA+AGG | 0.458077 | 8_1:-14873856 | None:intergenic |
| GCCTACTGAGTCCCGGCCTA+TGG | 0.458539 | 8_1:+14874286 | Msa1180670:CDS |
| CTATGATACAGATGTCTCTT+CGG | 0.468482 | 8_1:-14872971 | None:intergenic |
| CTTGTTTGTGAAGATCAATA+TGG | 0.475066 | 8_1:+14874364 | Msa1180670:CDS |
| AAGGAGACAAGATGCTTGTA+GGG | 0.476173 | 8_1:+14875213 | Msa1180670:CDS |
| TTCTTTGGAGGAGAGGAAGT+TGG | 0.476777 | 8_1:+14873615 | Msa1180670:CDS |
| ATTGACATCAATGCTTATGA+TGG | 0.483130 | 8_1:+14874410 | Msa1180670:CDS |
| CCTATTAGCAACTCTGGTAA+AGG | 0.483714 | 8_1:+14874341 | Msa1180670:CDS |
| CTTGTTTATGAAGATAATGA+AGG | 0.488627 | 8_1:+14875194 | Msa1180670:CDS |
| AACAAACCTATTAGCAACTC+TGG | 0.489814 | 8_1:+14874335 | Msa1180670:CDS |
| TACCAAACAGGTCTCAGAAA+AGG | 0.493666 | 8_1:+14873881 | Msa1180670:CDS |
| CATTGATTAAAGTTATAGCT+TGG | 0.495869 | 8_1:+14873121 | Msa1180670:five_prime_UTR |
| GAAATACTAAAACAATTAAA+CGG | 0.499774 | 8_1:+14873042 | Msa1180670:five_prime_UTR |
| GAAGAAGATAAAGGCATCAA+AGG | 0.503044 | 8_1:+14875143 | Msa1180670:CDS |
| GCAGAGATAAATTTATCTCA+TGG | 0.505424 | 8_1:+14875101 | Msa1180670:intron |
| ATCGGAGGCCAACCTACTAC+TGG | 0.509519 | 8_1:-14874218 | None:intergenic |
| GAGCTTAGACTTGGTCCACC+TGG | 0.509843 | 8_1:+14873637 | Msa1180670:CDS |
| GAAGAATCATCACTCACTCT+TGG | 0.524837 | 8_1:+14873715 | Msa1180670:CDS |
| GGCATCAAAGGATCATTGAC+TGG | 0.528381 | 8_1:+14875155 | Msa1180670:CDS |
| AAGCCACAAGTGTTCTTTGG+AGG | 0.532124 | 8_1:+14873603 | Msa1180670:CDS |
| TTCTTCCTGAATGAACGAAT+CGG | 0.538928 | 8_1:-14874236 | None:intergenic |
| AGGAAGTTGGAGCTTAGACT+TGG | 0.541596 | 8_1:+14873628 | Msa1180670:CDS |
| ACAAGTGTTCTTTGGAGGAG+AGG | 0.544453 | 8_1:+14873608 | Msa1180670:CDS |
| AATGCTGCTAGTCCAGTAGT+AGG | 0.545474 | 8_1:+14874206 | Msa1180670:CDS |
| CTGCTAGTCCAGTAGTAGGT+TGG | 0.549015 | 8_1:+14874210 | Msa1180670:CDS |
| TGCTTGTAGGGGACGTGCCG+TGG | 0.551530 | 8_1:+14875225 | Msa1180670:CDS |
| TTGACAAACTCTTCTTCACC+AGG | 0.560561 | 8_1:-14873655 | None:intergenic |
| CCTTGTCTTGAACCATAGGC+CGG | 0.561981 | 8_1:-14874298 | None:intergenic |
| CCGGCCTATGGTTCAAGACA+AGG | 0.569410 | 8_1:+14874298 | Msa1180670:CDS |
| TTCAGGAAGAACATTGCAAG+TGG | 0.570187 | 8_1:+14874248 | Msa1180670:CDS |
| TAAAACTCACGGTTAAAAGC+AGG | 0.572204 | 8_1:-14875591 | None:intergenic |
| TCTTGCAAGTTTGTGCAACA+AGG | 0.574470 | 8_1:-14873811 | None:intergenic |
| CTATTCCATGCTATATTCTG+TGG | 0.576490 | 8_1:+14873346 | Msa1180670:five_prime_UTR |
| ATAACCTTGTCTTGAACCAT+AGG | 0.576939 | 8_1:-14874302 | None:intergenic |
| TGGAATTAACAAGAAGCAAG+AGG | 0.591043 | 8_1:+14875121 | Msa1180670:CDS |
| CTAAGCCGCCTACTGAGTCC+CGG | 0.597620 | 8_1:+14874279 | Msa1180670:CDS |
| CTTGTCTTGAACCATAGGCC+GGG | 0.603934 | 8_1:-14874297 | None:intergenic |
| TTTGTGAAGATCAATATGGA+TGG | 0.610573 | 8_1:+14874368 | Msa1180670:CDS |
| TGTTTGTATCCACAGTGAAG+AGG | 0.617893 | 8_1:+14875541 | Msa1180670:CDS |
| TGATAAGATACTTACTGCCA+CGG | 0.622281 | 8_1:-14875242 | None:intergenic |
| ATGAGCCACAGAATATAGCA+TGG | 0.623301 | 8_1:-14873351 | None:intergenic |
| TCATTATAGGTTCTTCTGGG+AGG | 0.624893 | 8_1:+14873470 | Msa1180670:five_prime_UTR |
| TGTTGCACAAACTTGCAAGA+TGG | 0.633974 | 8_1:+14873814 | Msa1180670:CDS |
| TCTCCTCCAAAGAACACTTG+TGG | 0.635664 | 8_1:-14873606 | None:intergenic |
| ATAGGCCGGGACTCAGTAGG+CGG | 0.638385 | 8_1:-14874284 | None:intergenic |
| TTCCTGAATGAACGAATCGG+AGG | 0.657416 | 8_1:-14874233 | None:intergenic |
| ACAAACTCTTCTTCACCAGG+TGG | 0.675573 | 8_1:-14873652 | None:intergenic |
| AGGAGACAAGATGCTTGTAG+GGG | 0.714162 | 8_1:+14875214 | Msa1180670:CDS |
| CACACGCAGCCTCTTCACTG+TGG | 0.724956 | 8_1:-14875550 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATACTAAAACAATTAAA+CGG | + | chr8_1:14873042-14873061 | Msa1180670:five_prime_UTR | 15.0% |
| !! | TAACAAATTCAAAGTAAATA+AGG | - | chr8_1:14873382-14873401 | None:intergenic | 15.0% |
| !!! | GTATATTTTTAATGATCTAA+CGG | - | chr8_1:14874975-14874994 | None:intergenic | 15.0% |
| !! | TTAAAACAAAATTCACTTAA+AGG | - | chr8_1:14875034-14875053 | None:intergenic | 15.0% |
| !!! | ACTATAGTTTTAAAAAACAA+AGG | - | chr8_1:14875362-14875381 | None:intergenic | 15.0% |
| !!! | TAATTTTTAATGTGTATGTT+TGG | + | chr8_1:14875513-14875532 | Msa1180670:intron | 15.0% |
| !!! | TTTCAATTACATTTTTGTCT+CGG | - | chr8_1:14873026-14873045 | None:intergenic | 20.0% |
| !! | CAATGTTATAAAAACTGATT+TGG | - | chr8_1:14873106-14873125 | None:intergenic | 20.0% |
| !! | TGATAATAAACCTTTCTTTA+AGG | - | chr8_1:14873453-14873472 | None:intergenic | 20.0% |
| !! | GAAAGGTTTATTATCATTAT+AGG | + | chr8_1:14873457-14873476 | Msa1180670:five_prime_UTR | 20.0% |
| !!! | GTTTTTTCAAGATATCAAAA+AGG | + | chr8_1:14873495-14873514 | Msa1180670:five_prime_UTR | 20.0% |
| !! | AATTGTTTCAATCTAGAAAA+AGG | + | chr8_1:14873531-14873550 | Msa1180670:five_prime_UTR | 20.0% |
| !! | ATAAAGATGTAAATTGTTGT+TGG | + | chr8_1:14874001-14874020 | Msa1180670:intron | 20.0% |
| !!! | TTCACCAAACAAATATTTTA+AGG | + | chr8_1:14874895-14874914 | Msa1180670:intron | 20.0% |
| !! | TTGTCCTTAAAATATTTGTT+TGG | - | chr8_1:14874902-14874921 | None:intergenic | 20.0% |
| !! | AATGCTTTAGTTATGATAAT+TGG | + | chr8_1:14875467-14875486 | Msa1180670:intron | 20.0% |
| !!! | GTTATAAAAACTGATTTGGA+CGG | - | chr8_1:14873102-14873121 | None:intergenic | 25.0% |
| !! | CATTGATTAAAGTTATAGCT+TGG | + | chr8_1:14873121-14873140 | Msa1180670:five_prime_UTR | 25.0% |
| ! | TAACAAACCAAAAACAGAAA+GGG | - | chr8_1:14873232-14873251 | None:intergenic | 25.0% |
| !!! | TCTTTTTCATTCCTCTTTTT+AGG | - | chr8_1:14873293-14873312 | None:intergenic | 25.0% |
| ! | ATTATCATTATAGGTTCTTC+TGG | + | chr8_1:14873466-14873485 | Msa1180670:five_prime_UTR | 25.0% |
| ! | TTATCATTATAGGTTCTTCT+GGG | + | chr8_1:14873467-14873486 | Msa1180670:five_prime_UTR | 25.0% |
| ! | GACACAATTATTGCATTAAA+AGG | - | chr8_1:14874637-14874656 | None:intergenic | 25.0% |
| !! | TGGAAATGTTTTTCTTTGTT+GGG | + | chr8_1:14874719-14874738 | Msa1180670:intron | 25.0% |
| ! | TTTCAATCATAACTACTTCT+AGG | + | chr8_1:14875005-14875024 | Msa1180670:intron | 25.0% |
| ! | CTTGTTTATGAAGATAATGA+AGG | + | chr8_1:14875194-14875213 | Msa1180670:CDS | 25.0% |
| ! | CTATATATAACTCTTGCAAT+TGG | + | chr8_1:14875404-14875423 | Msa1180670:intron | 25.0% |
| ! | AAAACTCACACAATAACATA+GGG | - | chr8_1:14875435-14875454 | None:intergenic | 25.0% |
| AAAAACAGAAAGGGAATTAG+TGG | - | chr8_1:14873223-14873242 | None:intergenic | 30.0% | |
| ! | ACTAATTCCCTTTCTGTTTT+TGG | + | chr8_1:14873222-14873241 | Msa1180670:intron | 30.0% |
| CTAACAAACCAAAAACAGAA+AGG | - | chr8_1:14873233-14873252 | None:intergenic | 30.0% | |
| !!! | CTTTCTGTTTTTGGTTTGTT+AGG | + | chr8_1:14873231-14873250 | Msa1180670:intron | 30.0% |
| ! | CAAAGTTTTCTACTTCTTGA+TGG | + | chr8_1:14873419-14873438 | Msa1180670:five_prime_UTR | 30.0% |
| !! | GGTTTTTAAGCCTTAAAGAA+AGG | + | chr8_1:14873440-14873459 | Msa1180670:five_prime_UTR | 30.0% |
| ! | ATTTTGTAAGCAACAAGATG+TGG | + | chr8_1:14873575-14873594 | Msa1180670:exon | 30.0% |
| ATCAATGCAAGTGATGAAAA+AGG | + | chr8_1:14873771-14873790 | Msa1180670:CDS | 30.0% | |
| GATATAAGAGAGAAAAGCAA+AGG | - | chr8_1:14873911-14873930 | None:intergenic | 30.0% | |
| CTCTCTTATATCTATGTTTG+TGG | + | chr8_1:14873919-14873938 | Msa1180670:intron | 30.0% | |
| TATCAAGAACAACATTCACT+AGG | - | chr8_1:14873977-14873996 | None:intergenic | 30.0% | |
| CTTGTTTGTGAAGATCAATA+TGG | + | chr8_1:14874364-14874383 | Msa1180670:CDS | 30.0% | |
| TTTGTGAAGATCAATATGGA+TGG | + | chr8_1:14874368-14874387 | Msa1180670:CDS | 30.0% | |
| ! | ATGTCAATTTTTCTCCCAAT+AGG | - | chr8_1:14874398-14874417 | None:intergenic | 30.0% |
| ATTGACATCAATGCTTATGA+TGG | + | chr8_1:14874410-14874429 | Msa1180670:CDS | 30.0% | |
| ATTATCAACGTTTACCTTCA+AGG | - | chr8_1:14874481-14874500 | None:intergenic | 30.0% | |
| AATTATTGCATTAAAAGGCC+AGG | - | chr8_1:14874632-14874651 | None:intergenic | 30.0% | |
| !! | TGACATAACTTTTTGTTGTG+TGG | + | chr8_1:14874699-14874718 | Msa1180670:intron | 30.0% |
| ! | GTGGAAATGTTTTTCTTTGT+TGG | + | chr8_1:14874718-14874737 | Msa1180670:intron | 30.0% |
| CAAAAGATGTAGGTAACAAA+AGG | - | chr8_1:14874779-14874798 | None:intergenic | 30.0% | |
| GCTATTTAGTCAAAAGATGT+AGG | - | chr8_1:14874789-14874808 | None:intergenic | 30.0% | |
| !!! | GTTTCAATTGCATTTTAGCT+AGG | - | chr8_1:14874847-14874866 | None:intergenic | 30.0% |
| GCAGAGATAAATTTATCTCA+TGG | + | chr8_1:14875101-14875120 | Msa1180670:intron | 30.0% | |
| CAAAACTCACACAATAACAT+AGG | - | chr8_1:14875436-14875455 | None:intergenic | 30.0% | |
| !! | CATTTTTGTCTCGGTTTGAA+TGG | - | chr8_1:14873017-14873036 | None:intergenic | 35.0% |
| CGCAACTTGAAACAAATGTT+AGG | - | chr8_1:14873192-14873211 | None:intergenic | 35.0% | |
| !!! | GTTTTTGGTTTGTTAGGCTA+AGG | + | chr8_1:14873237-14873256 | Msa1180670:intron | 35.0% |
| TCAAACCTATGCCTAAAAAG+AGG | + | chr8_1:14873279-14873298 | Msa1180670:five_prime_UTR | 35.0% | |
| !! | TCATTCCTCTTTTTAGGCAT+AGG | - | chr8_1:14873287-14873306 | None:intergenic | 35.0% |
| ! | TTTGGTTTCACACGCTTAAA+TGG | - | chr8_1:14873317-14873336 | None:intergenic | 35.0% |
| ATGGAATAGTTGCCTAACTT+TGG | - | chr8_1:14873335-14873354 | None:intergenic | 35.0% | |
| CTATTCCATGCTATATTCTG+TGG | + | chr8_1:14873346-14873365 | Msa1180670:five_prime_UTR | 35.0% | |
| GAAATTCAGCTTTACCAAAC+AGG | + | chr8_1:14873869-14873888 | Msa1180670:CDS | 35.0% | |
| !!! | AGCAGCATTTCTGTTTTAGT+TGG | - | chr8_1:14874195-14874214 | None:intergenic | 35.0% |
| TTCTTCCTGAATGAACGAAT+CGG | - | chr8_1:14874239-14874258 | None:intergenic | 35.0% | |
| ATAACCTTGTCTTGAACCAT+AGG | - | chr8_1:14874305-14874324 | None:intergenic | 35.0% | |
| AACAAACCTATTAGCAACTC+TGG | + | chr8_1:14874335-14874354 | Msa1180670:CDS | 35.0% | |
| AATATGGATGGAGTTCCTAT+TGG | + | chr8_1:14874380-14874399 | Msa1180670:CDS | 35.0% | |
| ATATGGATGGAGTTCCTATT+GGG | + | chr8_1:14874381-14874400 | Msa1180670:CDS | 35.0% | |
| ATCTAACTCAATGTCTACCA+TGG | + | chr8_1:14874583-14874602 | Msa1180670:intron | 35.0% | |
| ATTGACTGTAGTAAATGCCA+TGG | - | chr8_1:14874603-14874622 | None:intergenic | 35.0% | |
| CAAGTAACATTCAAAACTCC+TGG | + | chr8_1:14874808-14874827 | Msa1180670:intron | 35.0% | |
| !! | CTAGGTTGACATTTTATACC+AGG | - | chr8_1:14874829-14874848 | None:intergenic | 35.0% |
| ATGATTGATTGACAGTGCAA+CGG | - | chr8_1:14874950-14874969 | None:intergenic | 35.0% | |
| CAATCATAACTACTTCTAGG+TGG | + | chr8_1:14875008-14875027 | Msa1180670:intron | 35.0% | |
| TGGAATTAACAAGAAGCAAG+AGG | + | chr8_1:14875121-14875140 | Msa1180670:CDS | 35.0% | |
| AAGCAAGAGGAAGAAGATAA+AGG | + | chr8_1:14875134-14875153 | Msa1180670:CDS | 35.0% | |
| !! | GAAGAAGATAAAGGCATCAA+AGG | + | chr8_1:14875143-14875162 | Msa1180670:CDS | 35.0% |
| TGATAAGATACTTACTGCCA+CGG | - | chr8_1:14875245-14875264 | None:intergenic | 35.0% | |
| !! | GAAGCTATGATTTGTTACTG+TGG | + | chr8_1:14875489-14875508 | Msa1180670:intron | 35.0% |
| TAAAACTCACGGTTAAAAGC+AGG | - | chr8_1:14875594-14875613 | None:intergenic | 35.0% | |
| TATAGCTTGGTCAACGATTC+AGG | + | chr8_1:14873134-14873153 | Msa1180670:five_prime_UTR | 40.0% | |
| AAGCGTGTGAAACCAAAGTT+AGG | + | chr8_1:14873320-14873339 | Msa1180670:five_prime_UTR | 40.0% | |
| ATGAGCCACAGAATATAGCA+TGG | - | chr8_1:14873354-14873373 | None:intergenic | 40.0% | |
| TCATTATAGGTTCTTCTGGG+AGG | + | chr8_1:14873470-14873489 | Msa1180670:five_prime_UTR | 40.0% | |
| AAGAAGCCACAAGTGTTCTT+TGG | + | chr8_1:14873600-14873619 | Msa1180670:CDS | 40.0% | |
| TTGACAAACTCTTCTTCACC+AGG | - | chr8_1:14873658-14873677 | None:intergenic | 40.0% | |
| GAAGAATCATCACTCACTCT+TGG | + | chr8_1:14873715-14873734 | Msa1180670:CDS | 40.0% | |
| TCTTGCAAGTTTGTGCAACA+AGG | - | chr8_1:14873814-14873833 | None:intergenic | 40.0% | |
| TGTTGCACAAACTTGCAAGA+TGG | + | chr8_1:14873814-14873833 | Msa1180670:CDS | 40.0% | |
| GCTGAATTTCTTGCAGAAGA+AGG | - | chr8_1:14873859-14873878 | None:intergenic | 40.0% | |
| TACCAAACAGGTCTCAGAAA+AGG | + | chr8_1:14873881-14873900 | Msa1180670:CDS | 40.0% | |
| !!! | TACCTTTTCTGAGACCTGTT+TGG | - | chr8_1:14873886-14873905 | None:intergenic | 40.0% |
| CAAGAACAACATTCACTAGG+CGG | - | chr8_1:14873974-14873993 | None:intergenic | 40.0% | |
| ATACCAACACCTTAACGTTG+TGG | + | chr8_1:14874061-14874080 | Msa1180670:intron | 40.0% | |
| CGTATCTGCAACCACAATTA+CGG | - | chr8_1:14874087-14874106 | None:intergenic | 40.0% | |
| TTCAGGAAGAACATTGCAAG+TGG | + | chr8_1:14874248-14874267 | Msa1180670:CDS | 40.0% | |
| CCTTTACCAGAGTTGCTAAT+AGG | - | chr8_1:14874344-14874363 | None:intergenic | 40.0% | |
| CCTATTAGCAACTCTGGTAA+AGG | + | chr8_1:14874341-14874360 | Msa1180670:CDS | 40.0% | |
| !! | GCTGTTGATGACCTATTTAG+AGG | + | chr8_1:14874452-14874471 | Msa1180670:CDS | 40.0% |
| CTTCAAGGAGACCTCTAAAT+AGG | - | chr8_1:14874466-14874485 | None:intergenic | 40.0% | |
| CTATTTAGAGGTCTCCTTGA+AGG | + | chr8_1:14874464-14874483 | Msa1180670:CDS | 40.0% | |
| ! | AAAGGATCATTGACTGGAAG+TGG | + | chr8_1:14875161-14875180 | Msa1180670:CDS | 40.0% |
| AAGGAGACAAGATGCTTGTA+GGG | + | chr8_1:14875213-14875232 | Msa1180670:CDS | 40.0% | |
| TGTTTGTATCCACAGTGAAG+AGG | + | chr8_1:14875541-14875560 | Msa1180670:CDS | 40.0% | |
| ! | CTTGGTCAACGATTCAGGTT+AGG | + | chr8_1:14873139-14873158 | Msa1180670:intron | 45.0% |
| ! | TTATAGGTTCTTCTGGGAGG+TGG | + | chr8_1:14873473-14873492 | Msa1180670:five_prime_UTR | 45.0% |
| AAGCCACAAGTGTTCTTTGG+AGG | + | chr8_1:14873603-14873622 | Msa1180670:CDS | 45.0% | |
| TCTCCTCCAAAGAACACTTG+TGG | - | chr8_1:14873609-14873628 | None:intergenic | 45.0% | |
| ! | ACAAGTGTTCTTTGGAGGAG+AGG | + | chr8_1:14873608-14873627 | Msa1180670:CDS | 45.0% |
| ! | TTCTTTGGAGGAGAGGAAGT+TGG | + | chr8_1:14873615-14873634 | Msa1180670:CDS | 45.0% |
| AGGAAGTTGGAGCTTAGACT+TGG | + | chr8_1:14873628-14873647 | Msa1180670:CDS | 45.0% | |
| ACAAACTCTTCTTCACCAGG+TGG | - | chr8_1:14873655-14873674 | None:intergenic | 45.0% | |
| CAATTACGGCCACAACGTTA+AGG | - | chr8_1:14874073-14874092 | None:intergenic | 45.0% | |
| TAACGTTGTGGCCGTAATTG+TGG | + | chr8_1:14874073-14874092 | Msa1180670:intron | 45.0% | |
| AATGCTGCTAGTCCAGTAGT+AGG | + | chr8_1:14874206-14874225 | Msa1180670:CDS | 45.0% | |
| TTCCTGAATGAACGAATCGG+AGG | - | chr8_1:14874236-14874255 | None:intergenic | 45.0% | |
| ACTACAGTCAATCCAGATCC+TGG | + | chr8_1:14874611-14874630 | Msa1180670:intron | 45.0% | |
| TGCATTAAAAGGCCAGGATC+TGG | - | chr8_1:14874626-14874645 | None:intergenic | 45.0% | |
| GGCATCAAAGGATCATTGAC+TGG | + | chr8_1:14875155-14875174 | Msa1180670:CDS | 45.0% | |
| GAAGGAGACAAGATGCTTGT+AGG | + | chr8_1:14875212-14875231 | Msa1180670:CDS | 45.0% | |
| AGGAGACAAGATGCTTGTAG+GGG | + | chr8_1:14875214-14875233 | Msa1180670:CDS | 45.0% | |
| AGCAGGAAGATCAGAACTCT+TGG | - | chr8_1:14875577-14875596 | None:intergenic | 45.0% | |
| CTGCTAGTCCAGTAGTAGGT+TGG | + | chr8_1:14874210-14874229 | Msa1180670:CDS | 50.0% | |
| CTTGTCTTGAACCATAGGCC+GGG | - | chr8_1:14874300-14874319 | None:intergenic | 50.0% | |
| CCTTGTCTTGAACCATAGGC+CGG | - | chr8_1:14874301-14874320 | None:intergenic | 50.0% | |
| ! | GAGCTTAGACTTGGTCCACC+TGG | + | chr8_1:14873637-14873656 | Msa1180670:CDS | 55.0% |
| CGGCCACAACGTTAAGGTGT+TGG | - | chr8_1:14874067-14874086 | None:intergenic | 55.0% | |
| ATCGGAGGCCAACCTACTAC+TGG | - | chr8_1:14874221-14874240 | None:intergenic | 55.0% | |
| ! | GGCCTCCGATTCGTTCATTC+AGG | + | chr8_1:14874231-14874250 | Msa1180670:CDS | 55.0% |
| CCGGCCTATGGTTCAAGACA+AGG | + | chr8_1:14874298-14874317 | Msa1180670:CDS | 55.0% | |
| CTAAGCCGCCTACTGAGTCC+CGG | + | chr8_1:14874279-14874298 | Msa1180670:CDS | 60.0% | |
| ATAGGCCGGGACTCAGTAGG+CGG | - | chr8_1:14874287-14874306 | None:intergenic | 60.0% | |
| ACCATAGGCCGGGACTCAGT+AGG | - | chr8_1:14874290-14874309 | None:intergenic | 60.0% | |
| CACACGCAGCCTCTTCACTG+TGG | - | chr8_1:14875553-14875572 | None:intergenic | 60.0% | |
| GCCTACTGAGTCCCGGCCTA+TGG | + | chr8_1:14874286-14874305 | Msa1180670:CDS | 65.0% | |
| TGCTTGTAGGGGACGTGCCG+TGG | + | chr8_1:14875225-14875244 | Msa1180670:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 14872972 | 14875614 | 14872972 | ID=Msa1180670;Name=Msa1180670 |
| chr8_1 | mRNA | 14872972 | 14875614 | 14872972 | ID=Msa1180670-mRNA-1;Parent=Msa1180670;Name=Msa1180670-mRNA-1;_AED=0.16;_eAED=0.16;_QI=523|1|0.8|1|0.75|0.6|5|0|270 |
| chr8_1 | exon | 14872972 | 14873155 | 14872972 | ID=Msa1180670-mRNA-1:exon:5512;Parent=Msa1180670-mRNA-1 |
| chr8_1 | exon | 14873253 | 14873902 | 14873253 | ID=Msa1180670-mRNA-1:exon:5513;Parent=Msa1180670-mRNA-1 |
| chr8_1 | exon | 14874205 | 14874485 | 14874205 | ID=Msa1180670-mRNA-1:exon:5514;Parent=Msa1180670-mRNA-1 |
| chr8_1 | exon | 14875105 | 14875249 | 14875105 | ID=Msa1180670-mRNA-1:exon:5515;Parent=Msa1180670-mRNA-1 |
| chr8_1 | exon | 14875539 | 14875614 | 14875539 | ID=Msa1180670-mRNA-1:exon:5516;Parent=Msa1180670-mRNA-1 |
| chr8_1 | five_prime_UTR | 14872972 | 14873155 | 14872972 | ID=Msa1180670-mRNA-1:five_prime_utr;Parent=Msa1180670-mRNA-1 |
| chr8_1 | five_prime_UTR | 14873253 | 14873591 | 14873253 | ID=Msa1180670-mRNA-1:five_prime_utr;Parent=Msa1180670-mRNA-1 |
| chr8_1 | CDS | 14873592 | 14873902 | 14873592 | ID=Msa1180670-mRNA-1:cds;Parent=Msa1180670-mRNA-1 |
| chr8_1 | CDS | 14874205 | 14874485 | 14874205 | ID=Msa1180670-mRNA-1:cds;Parent=Msa1180670-mRNA-1 |
| chr8_1 | CDS | 14875105 | 14875249 | 14875105 | ID=Msa1180670-mRNA-1:cds;Parent=Msa1180670-mRNA-1 |
| chr8_1 | CDS | 14875539 | 14875614 | 14875539 | ID=Msa1180670-mRNA-1:cds;Parent=Msa1180670-mRNA-1 |
| Gene Sequence |
| Protein sequence |
