AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0145600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0145600 MtrunA17_Chr1g0154441 98.985 394 4 0 94 487 1 394 0.0 771
Msa0145600 MtrunA17_Chr8g0368001 56.984 451 194 0 36 486 51 501 0.0 528
Msa0145600 MtrunA17_Chr5g0412771 54.244 483 212 2 8 481 20 502 5.10e-179 512
Msa0145600 MtrunA17_Chr1g0202761 50.000 496 238 4 1 487 1 495 2.12e-168 484
Msa0145600 MtrunA17_Chr5g0412541 49.669 453 227 1 28 479 29 481 1.09e-157 456
Msa0145600 MtrunA17_Chr3g0132531 50.862 464 227 1 20 482 31 494 2.04e-156 454
Msa0145600 MtrunA17_Chr2g0322671 48.980 490 234 4 4 479 10 497 1.13e-155 452
Msa0145600 MtrunA17_Chr4g0024501 47.106 501 243 5 3 481 11 511 1.47e-150 439
Msa0145600 MtrunA17_Chr7g0254521 47.289 461 243 0 27 487 23 483 4.38e-149 435
Msa0145600 MtrunA17_Chr7g0248051 47.992 498 235 4 4 478 22 518 4.85e-147 430
Msa0145600 MtrunA17_Chr7g0254511 46.739 460 242 2 21 480 18 474 1.47e-146 428
Msa0145600 MtrunA17_Chr2g0288281 52.196 387 184 1 94 479 1 387 4.98e-138 402
Msa0145600 MtrunA17_Chr6g0468521 42.138 477 272 3 3 479 1 473 4.61e-132 391
Msa0145600 MtrunA17_Chr6g0468531 42.558 477 271 2 3 479 1 474 7.12e-130 385
Msa0145600 MtrunA17_Chr5g0412571 51.026 390 190 1 92 480 38 427 5.71e-128 379
Msa0145600 MtrunA17_Chr6g0468511 42.558 477 270 3 3 479 1 473 5.40e-126 375
Msa0145600 MtrunA17_Chr7g0254531 46.891 386 205 0 94 479 1 386 6.91e-125 370
Msa0145600 MtrunA17_Chr5g0442331 45.416 469 252 2 14 478 22 490 1.26e-124 372
Msa0145600 MtrunA17_Chr6g0460541 47.727 396 206 1 92 487 3 397 1.98e-120 358
Msa0145600 MtrunA17_Chr1g0208791 39.158 475 279 5 12 478 15 487 1.01e-103 319
Msa0145600 MtrunA17_Chr6g0483361 39.440 464 266 6 20 476 14 469 2.94e-99 306
Msa0145600 MtrunA17_Chr6g0483381 37.578 479 288 5 1 476 1 471 2.22e-97 302
Msa0145600 MtrunA17_Chr3g0132541 35.789 475 234 6 12 478 18 429 3.42e-97 300
Msa0145600 MtrunA17_Chr7g0251331 38.235 442 272 1 36 476 27 468 1.44e-93 292
Msa0145600 MtrunA17_Chr6g0477961 36.952 479 291 4 1 476 1 471 1.84e-93 291
Msa0145600 MtrunA17_Chr7g0254501 48.675 302 154 1 179 480 2 302 1.62e-92 284
Msa0145600 MtrunA17_Chr6g0483331 38.031 447 268 5 37 478 15 457 5.39e-87 275
Msa0145600 MtrunA17_Chr1g0208821 35.404 483 296 7 3 476 8 483 1.67e-85 271
Msa0145600 MtrunA17_Chr8g0392601 37.104 442 276 2 38 478 16 456 3.47e-84 268
Msa0145600 MtrunA17_Chr6g0483351 39.211 380 228 2 94 470 1 380 3.46e-83 263
Msa0145600 MtrunA17_Chr6g0483341 35.938 448 276 5 37 478 44 486 1.22e-82 264
Msa0145600 MtrunA17_Chr6g0449061 34.989 443 286 2 36 476 31 473 3.28e-81 260
Msa0145600 MtrunA17_Chr1g0208881 36.425 442 280 1 36 476 26 467 2.29e-80 258
Msa0145600 MtrunA17_Chr2g0317131 34.110 472 297 5 4 467 11 476 2.56e-77 250
Msa0145600 MtrunA17_Chr1g0208891 34.842 442 287 1 36 476 25 466 2.33e-75 244
Msa0145600 MtrunA17_Chr2g0317151 35.104 433 280 1 36 467 34 466 1.54e-74 243
Msa0145600 MtrunA17_Chr2g0317171 34.047 467 300 3 4 467 9 470 4.38e-74 242
Msa0145600 MtrunA17_Chr2g0317161 35.566 433 278 1 36 467 32 464 8.60e-74 241
Msa0145600 MtrunA17_Chr6g0483161 35.789 380 224 2 92 470 1 361 4.63e-70 228
Msa0145600 MtrunA17_Chr2g0317221 34.828 379 244 2 91 467 7 384 1.11e-67 223
Msa0145600 MtrunA17_Chr7g0251321 33.679 386 236 2 94 478 1 367 5.88e-66 217
Msa0145600 MtrunA17_Chr5g0440661 37.875 367 164 5 94 459 1 304 4.35e-65 213
Msa0145600 MtrunA17_Chr6g0460531 46.586 249 131 2 24 271 13 260 2.06e-64 209
Msa0145600 MtrunA17_Chr5g0397991 30.562 445 296 5 36 473 66 504 6.33e-62 211
Msa0145600 MtrunA17_Chr3g0140931 28.814 472 327 4 12 477 26 494 1.05e-60 208
Msa0145600 MtrunA17_Chr6g0460521 50.476 210 104 0 270 479 1 210 7.72e-60 196
Msa0145600 MtrunA17_Chr8g0364761 39.033 269 162 2 36 302 54 322 3.53e-59 197
Msa0145600 MtrunA17_Chr1g0203791 29.325 474 322 5 6 474 39 504 4.05e-59 203
Msa0145600 MtrunA17_Chr6g0473701 28.571 490 334 5 1 482 91 572 2.53e-58 203
Msa0145600 MtrunA17_Chr5g0428251 28.125 448 311 4 36 477 56 498 8.35e-58 200
Msa0145600 MtrunA17_Chr1g0154451 95.652 92 4 0 1 92 1 92 1.73e-56 182
Msa0145600 MtrunA17_Chr8g0389801 27.563 439 315 2 36 473 24 460 7.87e-48 171
Msa0145600 MtrunA17_Chr6g0467321 27.494 451 313 5 36 474 43 491 1.55e-46 169
Msa0145600 MtrunA17_Chr8g0364751 50.000 184 92 0 302 485 1 184 3.00e-43 151
Msa0145600 MtrunA17_Chr4g0013031 25.652 460 319 5 26 472 57 506 1.55e-42 158
Msa0145600 MtrunA17_Chr6g0483264 37.981 208 129 0 273 480 1 208 6.43e-39 140
Msa0145600 MtrunA17_Chr5g0442311 40.223 179 106 1 94 271 1 179 1.77e-32 122
Msa0145600 MtrunA17_Chr2g0295631 23.362 458 334 3 36 476 21 478 2.11e-32 129
Msa0145600 MtrunA17_Chr6g0483221 36.913 149 94 0 320 468 6 154 1.80e-28 111
Msa0145600 MtrunA17_Chr6g0483261 37.267 161 101 0 320 480 6 166 1.79e-27 108
Msa0145600 MtrunA17_Chr6g0487091 25.627 359 246 5 128 473 39 389 5.77e-27 112
Msa0145600 MtrunA17_Chr7g0254491 49.505 101 49 1 21 121 18 116 5.16e-26 102
Msa0145600 MtrunA17_Chr6g0483241 36.957 184 109 3 92 271 1 181 1.90e-25 102
Msa0145600 MtrunA17_Chr5g0398571 29.870 231 160 1 245 473 56 286 5.58e-24 102
Msa0145600 MtrunA17_Chr5g0412561 39.535 86 43 2 6 91 16 92 1.73e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0145600 AT1G47530.1 67.925 477 150 2 6 482 8 481 0.0 657
Msa0145600 AT1G47530.2 71.465 389 111 0 94 482 1 389 0.0 572
Msa0145600 AT3G26590.1 54.725 455 206 0 30 484 39 493 0.0 518
Msa0145600 AT5G38030.1 53.347 478 217 2 13 484 16 493 7.39e-179 511
Msa0145600 AT1G23300.1 56.098 451 198 0 30 480 38 488 1.91e-177 508
Msa0145600 AT1G12950.1 54.405 454 207 0 28 481 59 512 3.31e-176 505
Msa0145600 AT4G00350.1 55.157 446 199 1 35 480 87 531 2.94e-172 496
Msa0145600 AT4G25640.1 51.572 477 222 3 14 482 4 479 2.16e-158 459
Msa0145600 AT4G25640.2 51.572 477 222 3 14 482 4 479 1.57e-157 457
Msa0145600 AT3G26590.2 53.708 391 181 0 94 484 1 391 2.16e-150 435
Msa0145600 AT3G21690.1 48.936 470 237 2 13 479 27 496 2.74e-150 439
Msa0145600 AT5G65380.1 48.712 466 238 1 20 484 21 486 5.34e-150 437
Msa0145600 AT1G61890.1 45.756 483 256 3 3 479 9 491 7.72e-146 427
Msa0145600 AT1G11670.1 47.661 449 234 1 35 482 49 497 5.00e-144 422
Msa0145600 AT4G25640.3 51.620 432 200 3 14 437 4 434 6.45e-143 418
Msa0145600 AT1G61890.2 45.781 474 251 3 3 470 9 482 1.09e-141 416
Msa0145600 AT1G33100.1 46.405 459 246 0 21 479 18 476 1.20e-141 416
Msa0145600 AT1G33110.1 46.070 458 247 0 22 479 22 479 4.68e-141 414
Msa0145600 AT5G44050.1 47.379 477 238 5 6 479 18 484 5.69e-141 414
Msa0145600 AT1G33090.1 46.507 458 245 0 22 479 22 479 3.22e-138 407
Msa0145600 AT5G10420.1 47.761 469 238 5 15 479 17 482 1.85e-137 405
Msa0145600 AT1G33080.1 44.978 458 252 0 22 479 22 479 1.02e-135 401
Msa0145600 AT4G21910.3 43.737 487 263 5 3 479 13 498 4.32e-134 397
Msa0145600 AT4G21910.1 43.737 487 263 5 3 479 13 498 4.32e-134 397
Msa0145600 AT4G21910.2 44.685 461 249 3 24 479 41 500 1.17e-133 396
Msa0145600 AT4G21910.4 44.685 461 249 3 24 479 41 500 1.17e-133 396
Msa0145600 AT3G59030.1 47.404 443 232 1 36 477 51 493 3.04e-133 395
Msa0145600 AT1G33080.2 45.133 452 248 0 22 473 22 473 4.21e-133 394
Msa0145600 AT4G21903.1 44.444 432 238 2 57 487 74 504 3.15e-129 385
Msa0145600 AT1G61890.3 44.695 443 239 3 3 439 9 451 2.47e-126 376
Msa0145600 AT4G21903.2 44.917 423 231 2 57 478 74 495 4.19e-126 377
Msa0145600 AT4G21903.3 45.570 395 214 1 94 487 1 395 1.55e-123 366
Msa0145600 AT5G17700.2 39.651 459 277 0 21 479 19 477 2.22e-116 352
Msa0145600 AT5G17700.1 39.651 459 277 0 21 479 19 477 2.22e-116 352
Msa0145600 AT3G03620.2 39.783 460 277 0 20 479 21 480 2.59e-115 349
Msa0145600 AT3G03620.1 39.783 460 277 0 20 479 21 480 2.59e-115 349
Msa0145600 AT1G33110.2 46.335 382 205 0 22 403 22 403 1.28e-111 336
Msa0145600 AT3G59030.2 46.175 366 196 1 36 400 51 416 1.85e-100 308
Msa0145600 AT1G73700.1 40.685 467 266 6 14 476 6 465 5.47e-98 303
Msa0145600 AT5G52450.1 41.176 442 258 2 38 478 29 469 3.32e-97 302
Msa0145600 AT2G34360.1 38.829 461 264 8 22 477 16 463 1.25e-88 280
Msa0145600 AT1G71140.1 36.771 446 273 3 36 476 26 467 1.10e-86 275
Msa0145600 AT5G17700.3 37.859 383 238 0 21 403 19 401 2.05e-85 269
Msa0145600 AT1G66760.2 32.568 479 306 5 2 476 4 469 1.96e-83 266
Msa0145600 AT3G23560.1 34.607 471 299 6 2 467 5 471 3.28e-82 263
Msa0145600 AT1G66760.1 32.627 472 299 6 2 468 4 461 4.99e-81 259
Msa0145600 AT1G66780.1 35.129 427 272 2 53 476 52 476 4.47e-80 257
Msa0145600 AT2G04080.1 32.823 457 300 2 32 487 23 473 4.84e-80 257
Msa0145600 AT1G15160.1 35.794 447 276 4 36 476 31 472 1.34e-79 256
Msa0145600 AT2G04040.1 32.649 487 312 5 3 487 1 473 1.56e-78 253
Msa0145600 AT1G15150.1 35.281 445 281 4 36 476 31 472 1.64e-78 253
Msa0145600 AT1G64820.1 33.781 447 281 5 38 476 31 470 4.35e-78 253
Msa0145600 AT2G04070.1 33.613 476 306 3 16 487 4 473 1.19e-77 251
Msa0145600 AT2G34360.2 35.652 460 238 6 22 477 16 421 1.21e-76 247
Msa0145600 AT2G04050.1 33.548 465 305 2 16 476 4 468 1.75e-76 248
Msa0145600 AT2G04080.2 32.648 438 294 1 32 468 23 460 5.09e-76 246
Msa0145600 AT1G15170.1 35.011 457 280 7 35 482 33 481 2.49e-75 245
Msa0145600 AT3G23550.1 33.623 461 299 4 14 467 3 463 5.16e-75 244
Msa0145600 AT2G04100.1 32.353 442 298 1 36 476 30 471 6.80e-72 236
Msa0145600 AT2G04090.1 31.448 442 302 1 36 476 30 471 1.84e-70 232
Msa0145600 AT1G15180.1 35.088 456 279 7 36 482 35 482 6.25e-70 231
Msa0145600 AT2G04100.2 32.110 436 295 1 36 470 30 465 3.29e-69 229
Msa0145600 AT1G15150.2 36.499 337 211 2 142 476 21 356 1.16e-65 216
Msa0145600 AT1G15170.2 36.311 347 210 4 142 482 21 362 3.54e-64 212
Msa0145600 AT1G58340.1 29.933 451 307 3 36 481 58 504 1.90e-60 207
Msa0145600 AT3G23560.2 32.921 404 262 6 2 400 5 404 6.23e-60 202
Msa0145600 AT1G66760.3 32.931 331 215 3 142 468 6 333 1.22e-58 197
Msa0145600 AT5G19700.1 26.907 472 332 3 16 474 4 475 8.95e-55 191
Msa0145600 AT1G66760.4 29.950 404 264 6 2 400 4 393 1.48e-53 185
Msa0145600 AT4G29140.1 26.609 466 341 1 10 474 30 495 1.43e-51 183
Msa0145600 AT2G38510.1 26.222 450 320 5 32 474 2 446 6.41e-47 170
Msa0145600 AT1G71870.1 25.103 486 336 6 8 472 1 479 6.73e-46 167
Msa0145600 AT4G23030.1 29.122 467 318 6 20 478 15 476 4.20e-45 165
Msa0145600 AT5G52050.1 25.942 451 315 2 36 473 35 479 6.52e-42 156
Msa0145600 AT5G49130.1 24.536 485 340 5 18 478 1 483 1.46e-40 152
Msa0145600 AT1G15180.2 35.094 265 161 4 142 400 21 280 6.38e-39 146
Msa0145600 AT4G22790.1 26.106 452 319 7 29 473 27 470 9.88e-36 139
Msa0145600 AT2G04066.1 38.843 121 74 0 356 476 43 163 2.45e-23 97.1

Find 114 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAATATTGAAACTTAAT+TGG 0.102187 1_4:-5297230 Msa0145600:CDS
TGATTTGTTATCAGGGTATT+TGG 0.215741 1_4:-5295780 Msa0145600:intron
TGGTTTCGCCTTTGGTTTCT+TGG 0.217826 1_4:-5298964 Msa0145600:intron
TTGGGCTTCACATTTGGTTT+TGG 0.259862 1_4:-5295990 Msa0145600:CDS
TCTAATATTGAAACTTAATT+GGG 0.293407 1_4:-5297229 Msa0145600:CDS
TTAATGAAGTAACAATTATA+AGG 0.295058 1_4:+5295719 None:intergenic
GTTGAAAATTCTGTTATTGC+TGG 0.307891 1_4:-5298984 Msa0145600:CDS
GTTATTGCTGGTTTCGCCTT+TGG 0.310201 1_4:-5298972 Msa0145600:CDS
CATTGGATGCTGTATCTATT+TGG 0.321661 1_4:-5296873 Msa0145600:intron
CAGCAAGTTTAGTTGTTTCC+TGG 0.333817 1_4:+5296254 None:intergenic
CTTGTAGCTTACATAAATCT+TGG 0.340357 1_4:-5296050 Msa0145600:CDS
TAGCTTCAGCTGTTATGTTA+TGG 0.343938 1_4:-5297053 Msa0145600:intron
GTGATGGCAGCTTGGAGTTT+TGG 0.345028 1_4:-5296948 Msa0145600:intron
CAGAAAGCCGTGTGAGGAAG+TGG 0.348907 1_4:-5295587 Msa0145600:CDS
ATGATATTGGTGGTGATAAC+AGG 0.354451 1_4:-5296919 Msa0145600:CDS
ATATTGTTGGGCTTCACATT+TGG 0.357452 1_4:-5295996 Msa0145600:CDS
GATAAAGAAGTTAAGATTGT+TGG 0.357514 1_4:+5299080 None:intergenic
CTTGGATCCTACTATGTTGT+TGG 0.361711 1_4:-5296032 Msa0145600:CDS
TTACCAGCTGGGATATTGTT+GGG 0.365810 1_4:-5296008 Msa0145600:CDS
AGTAACAATTATAAGGATTA+TGG 0.368933 1_4:+5295726 None:intergenic
GATTGCAGTATGAACATAAA+TGG 0.371621 1_4:-5296613 Msa0145600:intron
GGAGTTGCCCAAATATAAAA+AGG 0.371768 1_4:+5298568 None:intergenic
TTGTTACTTCATTAACCAAC+TGG 0.380634 1_4:-5295711 Msa0145600:CDS
AAACTTAATTGGGGCTTAGT+TGG 0.382505 1_4:-5297219 Msa0145600:CDS
GGAAAGTCCTGGTGATGTTA+TGG 0.383348 1_4:-5297296 Msa0145600:CDS
CTGAAAATTGTTCACAAAGT+TGG 0.384942 1_4:-5299137 Msa0145600:CDS
GGTGGTGATAACAGGGCATT+TGG 0.388031 1_4:-5296911 Msa0145600:CDS
ACTCCAAGCACCATCTGATT+TGG 0.390160 1_4:+5297128 None:intergenic
TGTTATCAGGGTATTTGGTC+AGG 0.396483 1_4:-5295775 Msa0145600:intron
GGTATTTGGTCAGGTCTGAT+TGG 0.397825 1_4:-5295766 Msa0145600:CDS
ATTACCAGCTGGGATATTGT+TGG 0.407188 1_4:-5296009 Msa0145600:CDS
GCATTCACTTCTACGTTTGT+TGG 0.412634 1_4:-5299041 Msa0145600:CDS
TGGTAGGCACATTTGCTTTG+TGG 0.414802 1_4:-5297383 Msa0145600:intron
AGGGCATTTGGCAAACCCTT+TGG 0.415152 1_4:-5296899 Msa0145600:CDS
AAAGCATATGCAAATATTTG+TGG 0.422937 1_4:+5297354 None:intergenic
TGGTTGGCAACAGTTGGGTA+TGG 0.428241 1_4:-5298707 Msa0145600:intron
ACACTATGTGGGCAAGCATT+TGG 0.433648 1_4:-5298670 Msa0145600:CDS
TGGATTATCTTGGTGGCTAC+AGG 0.437574 1_4:-5298604 Msa0145600:CDS
TTAGGTGTTGCTATTGGAGC+TGG 0.443556 1_4:-5296083 Msa0145600:intron
GGCAAACCCTTTGGTGCCAT+TGG 0.443751 1_4:-5296890 Msa0145600:CDS
TAGGGTTTAACGTTGCAATA+AGG 0.443944 1_4:-5296570 Msa0145600:intron
TGTTACATGTACCTCTTAGT+TGG 0.444285 1_4:-5297254 Msa0145600:CDS
GAATTTACATGCAAAGATCA+TGG 0.447765 1_4:-5298624 Msa0145600:CDS
AGCAAGTTTAGTTGTTTCCT+GGG 0.447936 1_4:+5296255 None:intergenic
TATGTTGTTGGATTACCAGC+TGG 0.453772 1_4:-5296020 Msa0145600:CDS
TGCAAAGTTCTCAGTGATAG+TGG 0.455040 1_4:-5296377 Msa0145600:CDS
GCAGTATGAACATAAATGGT+TGG 0.464973 1_4:-5296609 Msa0145600:intron
CAGTATGAACATAAATGGTT+GGG 0.468025 1_4:-5296608 Msa0145600:CDS
TCATGGTGGTTGATTGTCAT+TGG 0.473305 1_4:-5297174 Msa0145600:CDS
GCAAAGATCATGGATTATCT+TGG 0.476538 1_4:-5298614 Msa0145600:CDS
AGCCTACAACCAGTCTTGTC+TGG 0.477429 1_4:-5296202 Msa0145600:intron
TGGGATATGATGATCGCGCT+AGG 0.486272 1_4:-5296589 Msa0145600:CDS
TGGTAATCCAACAACATAGT+AGG 0.486946 1_4:+5296025 None:intergenic
AGAAGTTAAGATTGTTGGAC+CGG 0.486994 1_4:+5299085 None:intergenic
AATATTAGAAGCCAACTAAG+AGG 0.488197 1_4:+5297243 None:intergenic
CAATTATTCTCAACACATCA+TGG 0.498659 1_4:-5297191 Msa0145600:CDS
ATTTGGTCAGGTCTGATTGG+AGG 0.508178 1_4:-5295763 Msa0145600:CDS
TCACCAAATCAGATGGTGCT+TGG 0.510768 1_4:-5297131 Msa0145600:CDS
TCTTTATCTCAGTACTCGCT+TGG 0.519020 1_4:-5299065 Msa0145600:CDS
GTGATGTTATGGATATCATT+AGG 0.521877 1_4:-5297285 Msa0145600:CDS
GGTTGGCAACAGTTGGGTAT+GGG 0.521926 1_4:-5298706 Msa0145600:intron
GTTGGGTATGGGAAGTGCAT+TGG 0.523324 1_4:-5298695 Msa0145600:CDS
AGTTGTTAGTACTATGATCA+TGG 0.530879 1_4:+5296321 None:intergenic
GATGAGATCGCAGAAGCTGC+TGG 0.531826 1_4:-5298520 Msa0145600:intron
TTCCTCCCCACTTCCTCACA+CGG 0.534562 1_4:+5295580 None:intergenic
TGCTGGTCATATAAGAATGA+TGG 0.538828 1_4:-5298647 Msa0145600:CDS
TAGCTTGAAGTGAACAATTG+AGG 0.540499 1_4:+5296286 None:intergenic
GAGTGGAGTCGAAGAAGCTA+TGG 0.541517 1_4:-5299115 Msa0145600:CDS
TTCAAGCTATGAGGTTGCCC+AGG 0.542260 1_4:-5296272 Msa0145600:CDS
ACAGCATCCAATGGCACCAA+AGG 0.544225 1_4:+5296883 None:intergenic
AACCAGACAAGACTGGTTGT+AGG 0.545104 1_4:+5296200 None:intergenic
ATTGTTCACTTCAAGCTATG+AGG 0.547129 1_4:-5296281 Msa0145600:CDS
CAAGATCGAGCTCGTTAACA+TGG 0.548496 1_4:+5299018 None:intergenic
TCTCTCACATCAATATCCAT+TGG 0.549511 1_4:-5296352 Msa0145600:CDS
TGTGACTAACCAGACAAGAC+TGG 0.551439 1_4:+5296193 None:intergenic
GAAAGCCGTGTGAGGAAGTG+GGG 0.552917 1_4:-5295585 Msa0145600:CDS
ACTCCCCTCTTGAAGCTCGT+TGG 0.555491 1_4:-5298550 Msa0145600:CDS
AGAAAGCCGTGTGAGGAAGT+GGG 0.557799 1_4:-5295586 Msa0145600:CDS
AAGATCATGGATTATCTTGG+TGG 0.557828 1_4:-5298611 Msa0145600:CDS
GTATCAAATGAACTTGGAGC+AGG 0.561306 1_4:-5296412 Msa0145600:CDS
AAGCCCAACAATATCCCAGC+TGG 0.562621 1_4:+5296005 None:intergenic
AATTGTTCACAAAGTTGGAG+TGG 0.564145 1_4:-5299132 Msa0145600:CDS
GTGCTTGGAGTGGATTCTCA+TGG 0.564381 1_4:-5297116 Msa0145600:CDS
ATGTTGTTGGATTACCAGCT+GGG 0.564636 1_4:-5296019 Msa0145600:CDS
GTGAGAGTATCAAATGAACT+TGG 0.567071 1_4:-5296418 Msa0145600:CDS
TTGACATTGTTATCTAGTAG+AGG 0.570811 1_4:+5299200 None:intergenic
CTAATATTGAAACTTAATTG+GGG 0.574308 1_4:-5297228 Msa0145600:CDS
ACAAGCACAAAGGAAAGTCC+TGG 0.574888 1_4:-5297307 Msa0145600:CDS
AAATCAGATGGTGCTTGGAG+TGG 0.576701 1_4:-5297126 Msa0145600:CDS
GGGATATGATGATCGCGCTA+GGG 0.580089 1_4:-5296588 Msa0145600:CDS
TGTGGGCAAGCATTTGGTGC+TGG 0.581549 1_4:-5298664 Msa0145600:CDS
CAGCATCCAATGGCACCAAA+GGG 0.581611 1_4:+5296884 None:intergenic
TTGGTACTTGATGATATTGG+TGG 0.583882 1_4:-5296929 Msa0145600:CDS
GATCATGGCAACAACACCAA+TGG 0.586370 1_4:+5296336 None:intergenic
AGTGCATTGGAGACACTATG+TGG 0.586848 1_4:-5298682 Msa0145600:CDS
TCCTAGTGTTGTTCATCATG+AGG 0.592021 1_4:-5299171 Msa0145600:CDS
AAGAAGCTATGGAAAATAGC+CGG 0.605436 1_4:-5299104 Msa0145600:CDS
CTGTCCAACGAGCTTCAAGA+GGG 0.606132 1_4:+5298546 None:intergenic
GCTGGTCATATAAGAATGAT+GGG 0.606977 1_4:-5298646 Msa0145600:CDS
TCCTCATGATGAACAACACT+AGG 0.609928 1_4:+5299170 None:intergenic
TAGAGGACTGTCGATAGCCA+TGG 0.613803 1_4:+5299217 None:intergenic
ATGATATCCATAACATCACC+AGG 0.617590 1_4:+5297289 None:intergenic
CAAATAGATACAGCATCCAA+TGG 0.632386 1_4:+5296874 None:intergenic
TCTGTCCAACGAGCTTCAAG+AGG 0.635922 1_4:+5298545 None:intergenic
AAAAGTTCTTACAAGCACAA+AGG 0.640238 1_4:-5297317 Msa0145600:CDS
TTTACATACCAAGAAACCAA+AGG 0.654334 1_4:+5298956 None:intergenic
ATGAAGCAGAAAGCCGTGTG+AGG 0.654673 1_4:-5295593 Msa0145600:CDS
TCATGATGAACAACACTAGG+AGG 0.658187 1_4:+5299173 None:intergenic
GTGTTGCTATTGGAGCTGGT+TGG 0.658329 1_4:-5296079 Msa0145600:CDS
GTGCATTGGAGACACTATGT+GGG 0.665609 1_4:-5298681 Msa0145600:CDS
TGATATTGGTGGTGATAACA+GGG 0.675304 1_4:-5296918 Msa0145600:CDS
AGCCGTGTGAGGAAGTGGGG+AGG 0.676849 1_4:-5295582 Msa0145600:CDS
TGTCCAACGAGCTTCAAGAG+GGG 0.697789 1_4:+5298547 None:intergenic
TTATTCTCAACACATCATGG+TGG 0.744685 1_4:-5297188 Msa0145600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAAAATTGTTTTTGTTTT+TGG - chr1_4:5296048-5296067 Msa0145600:CDS 10.0%
!!! TATATAGTATAATTTTTAGA+AGG - chr1_4:5298866-5298885 Msa0145600:intron 10.0%
!!! AGGATACATTTTTTTTTTTT+TGG + chr1_4:5296015-5296034 None:intergenic 15.0%
!!! TTTTATAAATCATAAACTTG+AGG + chr1_4:5296035-5296054 None:intergenic 15.0%
!! ATATTCCTATCATTTAAATT+TGG - chr1_4:5296297-5296316 Msa0145600:CDS 15.0%
!! ATTTACAAGAGAAATATTTA+CGG + chr1_4:5296351-5296370 None:intergenic 15.0%
!!! TATTTTATCAATTTTCCTTT+TGG - chr1_4:5297372-5297391 Msa0145600:CDS 15.0%
!! TTCTAATATTGAAACTTAAT+TGG - chr1_4:5297545-5297564 Msa0145600:intron 15.0%
!! TCTAATATTGAAACTTAATT+GGG - chr1_4:5297546-5297565 Msa0145600:intron 15.0%
!! ATTAAATTCAAGAGAAATAA+GGG + chr1_4:5298830-5298849 None:intergenic 15.0%
!! TATTAAATTCAAGAGAAATA+AGG + chr1_4:5298831-5298850 None:intergenic 15.0%
!!! ATATTTATTGTTTGTTAATG+AGG - chr1_4:5298906-5298925 Msa0145600:intron 15.0%
!! TTAATGAAGTAACAATTATA+AGG + chr1_4:5299059-5299078 None:intergenic 15.0%
!!! ATTAATTTTCAACAAACAAT+AGG + chr1_4:5299136-5299155 None:intergenic 15.0%
!! GAAAATTAATTTCATATCTT+TGG - chr1_4:5299146-5299165 Msa0145600:CDS 15.0%
!! AGTGTAATGTAATAATAAAG+CGG + chr1_4:5295857-5295876 None:intergenic 20.0%
!!! ACACTTATTTTTGTTCTTTA+AGG - chr1_4:5295872-5295891 Msa0145600:intron 20.0%
!!! AAATTGTTTTTGTTTTTGGT+TGG - chr1_4:5296052-5296071 Msa0145600:CDS 20.0%
!!! TGTTGTTACCTTTTTATATT+TGG - chr1_4:5296199-5296218 Msa0145600:intron 20.0%
!!! GTTGTTACCTTTTTATATTT+GGG - chr1_4:5296200-5296219 Msa0145600:intron 20.0%
!! TTAGTCCAAATTTAAATGAT+AGG + chr1_4:5296305-5296324 None:intergenic 20.0%
!!! TTGTTACTATTTAGTTTGAA+GGG - chr1_4:5296385-5296404 Msa0145600:CDS 20.0%
!! TAACTTACTAACTATAATGA+TGG + chr1_4:5296638-5296657 None:intergenic 20.0%
!! AGTAAGTTAATTTAAGTAGT+TGG - chr1_4:5296649-5296668 Msa0145600:intron 20.0%
!! AGTTTACATGTAAAAAACTT+CGG - chr1_4:5296892-5296911 Msa0145600:CDS 20.0%
!!! TAAAACTATGATAATGACTT+GGG - chr1_4:5297079-5297098 Msa0145600:CDS 20.0%
!!! GATATTTATTGATTCAATAG+TGG - chr1_4:5297232-5297251 Msa0145600:CDS 20.0%
!! CTAATATTGAAACTTAATTG+GGG - chr1_4:5297547-5297566 Msa0145600:intron 20.0%
!! TCAGCTTAATTAATATTTGA+TGG - chr1_4:5297774-5297793 Msa0145600:intron 20.0%
!!! ATTGAATTTTGAAAATGTGA+TGG - chr1_4:5297811-5297830 Msa0145600:intron 20.0%
!!! AAATGTCTATCACTTTTATT+TGG + chr1_4:5297975-5297994 None:intergenic 20.0%
!!! TAAATTGTTCACTTTAAAGA+AGG + chr1_4:5298270-5298289 None:intergenic 20.0%
!! TTAAATTCAAGAGAAATAAG+GGG + chr1_4:5298829-5298848 None:intergenic 20.0%
!! AGTAACAATTATAAGGATTA+TGG + chr1_4:5299052-5299071 None:intergenic 20.0%
! GATAAAGAAGTTAAGATTGT+TGG + chr1_4:5295698-5295717 None:intergenic 25.0%
! AAGAGAAATATTTACGGAAA+TGG + chr1_4:5296345-5296364 None:intergenic 25.0%
!!! CTTGTTACTATTTAGTTTGA+AGG - chr1_4:5296384-5296403 Msa0145600:CDS 25.0%
! GATCGTTCAATGTAAATTAA+CGG - chr1_4:5296514-5296533 Msa0145600:intron 25.0%
!! TAAAGGGATAGTTAATTTGA+AGG - chr1_4:5296582-5296601 Msa0145600:CDS 25.0%
!! AAAGGGATAGTTAATTTGAA+GGG - chr1_4:5296583-5296602 Msa0145600:CDS 25.0%
!!! TCCATTTTTCGCTAATTAAA+AGG - chr1_4:5296777-5296796 Msa0145600:intron 25.0%
!!! CCATTTTTCGCTAATTAAAA+GGG - chr1_4:5296778-5296797 Msa0145600:intron 25.0%
! AAAGTAGTATATTTACCCTT+TGG + chr1_4:5296844-5296863 None:intergenic 25.0%
!!! GGTAAATATACTACTTTTGT+GGG - chr1_4:5296847-5296866 Msa0145600:intron 25.0%
!! CATTTAAAATTTCCTAGCAA+GGG + chr1_4:5296987-5297006 None:intergenic 25.0%
!! TTGCTAGGAAATTTTAAATG+CGG - chr1_4:5296987-5297006 Msa0145600:intron 25.0%
!! GTAAAACTATGATAATGACT+TGG - chr1_4:5297078-5297097 Msa0145600:CDS 25.0%
!! AAAACTATGATAATGACTTG+GGG - chr1_4:5297080-5297099 Msa0145600:CDS 25.0%
!! TTATCAATTTTCCTTTTGGT+AGG - chr1_4:5297376-5297395 Msa0145600:CDS 25.0%
! AAAGCATATGCAAATATTTG+TGG + chr1_4:5297424-5297443 None:intergenic 25.0%
! TTCAAAACTGAGCATTAATT+AGG - chr1_4:5298038-5298057 Msa0145600:intron 25.0%
!!! GCATTTGTTGTTACTATTTT+AGG - chr1_4:5298674-5298693 Msa0145600:CDS 25.0%
!!! TATACCTATGATTTGTTATC+AGG - chr1_4:5298987-5299006 Msa0145600:CDS 25.0%
!!! ATACCTATGATTTGTTATCA+GGG - chr1_4:5298988-5299007 Msa0145600:CDS 25.0%
TTGACATTGTTATCTAGTAG+AGG + chr1_4:5295578-5295597 None:intergenic 30.0%
! ACTTTGTGAACAATTTTCAG+TGG + chr1_4:5295638-5295657 None:intergenic 30.0%
CTGAAAATTGTTCACAAAGT+TGG - chr1_4:5295638-5295657 Msa0145600:intron 30.0%
GTTGAAAATTCTGTTATTGC+TGG - chr1_4:5295791-5295810 Msa0145600:intron 30.0%
TTTACATACCAAGAAACCAA+AGG + chr1_4:5295822-5295841 None:intergenic 30.0%
! TTGAAAAATGTTGAGTTGTG+TGG - chr1_4:5295920-5295939 Msa0145600:intron 30.0%
GAATTTACATGCAAAGATCA+TGG - chr1_4:5296151-5296170 Msa0145600:intron 30.0%
! TCCATCTTTTCACTAGTTAA+AGG - chr1_4:5296565-5296584 Msa0145600:intron 30.0%
! CCATCTTTTCACTAGTTAAA+GGG - chr1_4:5296566-5296585 Msa0145600:intron 30.0%
!! CCCTTTTAATTAGCGAAAAA+TGG + chr1_4:5296781-5296800 None:intergenic 30.0%
!! GGGTAAATATACTACTTTTG+TGG - chr1_4:5296846-5296865 Msa0145600:intron 30.0%
ATAAAAATTTGCAGCGTAAC+TGG - chr1_4:5296918-5296937 Msa0145600:CDS 30.0%
! GCATTTAAAATTTCCTAGCA+AGG + chr1_4:5296988-5297007 None:intergenic 30.0%
! TAATGACCTGGTCAATTTTA+TGG + chr1_4:5297300-5297319 None:intergenic 30.0%
! GCTTGTAAGAACTTTTGAAT+TGG + chr1_4:5297454-5297473 None:intergenic 30.0%
AAAAGTTCTTACAAGCACAA+AGG - chr1_4:5297458-5297477 Msa0145600:intron 30.0%
GTGATGTTATGGATATCATT+AGG - chr1_4:5297490-5297509 Msa0145600:intron 30.0%
AATATTAGAAGCCAACTAAG+AGG + chr1_4:5297535-5297554 None:intergenic 30.0%
CAATTATTCTCAACACATCA+TGG - chr1_4:5297584-5297603 Msa0145600:intron 30.0%
! ATTTTCATCACCAAATCAGA+TGG - chr1_4:5297637-5297656 Msa0145600:intron 30.0%
!! GTTTTGGTACTTGATGATAT+TGG - chr1_4:5297843-5297862 Msa0145600:intron 30.0%
ACAATGAGAATTTGGAGAAA+GGG + chr1_4:5297932-5297951 None:intergenic 30.0%
TCTATAGCACAATGAGAATT+TGG + chr1_4:5297940-5297959 None:intergenic 30.0%
TCATTGTGCTATAGAAGTTA+TGG - chr1_4:5297945-5297964 Msa0145600:intron 30.0%
TTATGTTCATACTGCAATCA+TGG + chr1_4:5298161-5298180 None:intergenic 30.0%
GATTGCAGTATGAACATAAA+TGG - chr1_4:5298162-5298181 Msa0145600:intron 30.0%
CAGTATGAACATAAATGGTT+GGG - chr1_4:5298167-5298186 Msa0145600:intron 30.0%
AGTTGTTAGTACTATGATCA+TGG + chr1_4:5298457-5298476 None:intergenic 30.0%
!!! ACTATTTTAGGTGTTGCTAT+TGG - chr1_4:5298686-5298705 Msa0145600:CDS 30.0%
CTTGTAGCTTACATAAATCT+TGG - chr1_4:5298725-5298744 Msa0145600:intron 30.0%
ATACCCTGATAACAAATCAT+AGG + chr1_4:5298994-5299013 None:intergenic 30.0%
!! TGATTTGTTATCAGGGTATT+TGG - chr1_4:5298995-5299014 Msa0145600:CDS 30.0%
! TTGTTACTTCATTAACCAAC+TGG - chr1_4:5299064-5299083 Msa0145600:CDS 30.0%
ATTAACCAACTGGAAAAAAG+AGG - chr1_4:5299074-5299093 Msa0145600:CDS 30.0%
TTCATATCTTTGGATTTCAC+AGG - chr1_4:5299156-5299175 Msa0145600:CDS 30.0%
AATTGTTCACAAAGTTGGAG+TGG - chr1_4:5295643-5295662 Msa0145600:intron 35.0%
AAGAAGCTATGGAAAATAGC+CGG - chr1_4:5295671-5295690 Msa0145600:intron 35.0%
AGAAGTTAAGATTGTTGGAC+CGG + chr1_4:5295693-5295712 None:intergenic 35.0%
TGCTGGTCATATAAGAATGA+TGG - chr1_4:5296128-5296147 Msa0145600:intron 35.0%
GCTGGTCATATAAGAATGAT+GGG - chr1_4:5296129-5296148 Msa0145600:intron 35.0%
! GCAAAGATCATGGATTATCT+TGG - chr1_4:5296161-5296180 Msa0145600:intron 35.0%
! AAGATCATGGATTATCTTGG+TGG - chr1_4:5296164-5296183 Msa0145600:intron 35.0%
GGAGTTGCCCAAATATAAAA+AGG + chr1_4:5296210-5296229 None:intergenic 35.0%
AAAGTCACAATGAAGACGTA+CGG + chr1_4:5296472-5296491 None:intergenic 35.0%
TACATTGAACGATCGACATT+GGG + chr1_4:5296508-5296527 None:intergenic 35.0%
TTACATTGAACGATCGACAT+TGG + chr1_4:5296509-5296528 None:intergenic 35.0%
CCCTTTAACTAGTGAAAAGA+TGG + chr1_4:5296569-5296588 None:intergenic 35.0%
! TATGACTACAAGAGTACACA+AGG - chr1_4:5296746-5296765 Msa0145600:intron 35.0%
TTAAAAGGGTAGCTAGGATT+AGG - chr1_4:5296792-5296811 Msa0145600:intron 35.0%
TAAAAGGGTAGCTAGGATTA+GGG - chr1_4:5296793-5296812 Msa0145600:intron 35.0%
! TAATGGCTTGTGTGTTTGTT+TGG - chr1_4:5297325-5297344 Msa0145600:CDS 35.0%
ATGATATCCATAACATCACC+AGG + chr1_4:5297489-5297508 None:intergenic 35.0%
TGTTACATGTACCTCTTAGT+TGG - chr1_4:5297521-5297540 Msa0145600:intron 35.0%
AAACTTAATTGGGGCTTAGT+TGG - chr1_4:5297556-5297575 Msa0145600:intron 35.0%
TTATTCTCAACACATCATGG+TGG - chr1_4:5297587-5297606 Msa0145600:intron 35.0%
TAGCTTCAGCTGTTATGTTA+TGG - chr1_4:5297722-5297741 Msa0145600:intron 35.0%
! TTGGTACTTGATGATATTGG+TGG - chr1_4:5297846-5297865 Msa0145600:intron 35.0%
!! ATGATATTGGTGGTGATAAC+AGG - chr1_4:5297856-5297875 Msa0145600:intron 35.0%
!! TGATATTGGTGGTGATAACA+GGG - chr1_4:5297857-5297876 Msa0145600:intron 35.0%
CAAATAGATACAGCATCCAA+TGG + chr1_4:5297904-5297923 None:intergenic 35.0%
CATTGGATGCTGTATCTATT+TGG - chr1_4:5297902-5297921 Msa0145600:intron 35.0%
CACAATGAGAATTTGGAGAA+AGG + chr1_4:5297933-5297952 None:intergenic 35.0%
AACTGAGCATTAATTAGGCT+TGG - chr1_4:5298043-5298062 Msa0145600:intron 35.0%
ACTGAGCATTAATTAGGCTT+GGG - chr1_4:5298044-5298063 Msa0145600:intron 35.0%
TCATCCAATTGCAGTATACA+AGG - chr1_4:5298085-5298104 Msa0145600:intron 35.0%
TTCTCCTTGTATACTGCAAT+TGG + chr1_4:5298092-5298111 None:intergenic 35.0%
GCAGTATGAACATAAATGGT+TGG - chr1_4:5298166-5298185 Msa0145600:intron 35.0%
TAGGGTTTAACGTTGCAATA+AGG - chr1_4:5298205-5298224 Msa0145600:intron 35.0%
!! GTGAGAGTATCAAATGAACT+TGG - chr1_4:5298357-5298376 Msa0145600:intron 35.0%
TCTCTCACATCAATATCCAT+TGG - chr1_4:5298423-5298442 Msa0145600:intron 35.0%
TAGCTTGAAGTGAACAATTG+AGG + chr1_4:5298492-5298511 None:intergenic 35.0%
ATTGTTCACTTCAAGCTATG+AGG - chr1_4:5298494-5298513 Msa0145600:intron 35.0%
! AGCAAGTTTAGTTGTTTCCT+GGG + chr1_4:5298523-5298542 None:intergenic 35.0%
TGGTAATCCAACAACATAGT+AGG + chr1_4:5298753-5298772 None:intergenic 35.0%
ATATTGTTGGGCTTCACATT+TGG - chr1_4:5298779-5298798 Msa0145600:intron 35.0%
!!! ACTAACCTCTTTTTTCCAGT+TGG + chr1_4:5299082-5299101 None:intergenic 35.0%
TCATGATGAACAACACTAGG+AGG + chr1_4:5295605-5295624 None:intergenic 40.0%
! TCCTAGTGTTGTTCATCATG+AGG - chr1_4:5295604-5295623 Msa0145600:intron 40.0%
TCCTCATGATGAACAACACT+AGG + chr1_4:5295608-5295627 None:intergenic 40.0%
TCTTTATCTCAGTACTCGCT+TGG - chr1_4:5295710-5295729 Msa0145600:CDS 40.0%
GCATTCACTTCTACGTTTGT+TGG - chr1_4:5295734-5295753 Msa0145600:CDS 40.0%
GAATTGTGTGTTAGAACGCA+CGG - chr1_4:5295942-5295961 Msa0145600:intron 40.0%
AATTGTGTGTTAGAACGCAC+GGG - chr1_4:5295943-5295962 Msa0145600:intron 40.0%
!!! TGTTTTTGGTTGGCAACAGT+TGG - chr1_4:5296062-5296081 Msa0145600:CDS 40.0%
!!! GTTTTTGGTTGGCAACAGTT+GGG - chr1_4:5296063-5296082 Msa0145600:CDS 40.0%
GTTTGAAGGGCTTAAATCGT+CGG - chr1_4:5296398-5296417 Msa0145600:CDS 40.0%
CGGTCGAGATTATGACTATA+TGG - chr1_4:5296534-5296553 Msa0145600:intron 40.0%
AACTCAAAATGTCGATCGTC+TGG - chr1_4:5296703-5296722 Msa0145600:intron 40.0%
CGCTAATTAAAAGGGTAGCT+AGG - chr1_4:5296786-5296805 Msa0145600:intron 40.0%
AAAATTTCCTAGCAAGGGCA+AGG + chr1_4:5296982-5297001 None:intergenic 40.0%
TGTGCACCATAAAATTGACC+AGG - chr1_4:5297291-5297310 Msa0145600:CDS 40.0%
ACCAGGTCATTACACGATAA+TGG - chr1_4:5297308-5297327 Msa0145600:CDS 40.0%
AAGCAAATGTGCCTACCAAA+AGG + chr1_4:5297390-5297409 None:intergenic 40.0%
TCATGGTGGTTGATTGTCAT+TGG - chr1_4:5297601-5297620 Msa0145600:intron 40.0%
TTGAAAATGTGATGGCAGCT+TGG - chr1_4:5297819-5297838 Msa0145600:intron 40.0%
!! CACAACAATGCAAAAGTGCT+AGG + chr1_4:5298009-5298028 None:intergenic 40.0%
GTATCAAATGAACTTGGAGC+AGG - chr1_4:5298363-5298382 Msa0145600:intron 40.0%
TGCAAAGTTCTCAGTGATAG+TGG - chr1_4:5298398-5298417 Msa0145600:intron 40.0%
! CAGCAAGTTTAGTTGTTTCC+TGG + chr1_4:5298524-5298543 None:intergenic 40.0%
CTTGGATCCTACTATGTTGT+TGG - chr1_4:5298743-5298762 Msa0145600:intron 40.0%
TATGTTGTTGGATTACCAGC+TGG - chr1_4:5298755-5298774 Msa0145600:intron 40.0%
ATGTTGTTGGATTACCAGCT+GGG - chr1_4:5298756-5298775 Msa0145600:intron 40.0%
ATTACCAGCTGGGATATTGT+TGG - chr1_4:5298766-5298785 Msa0145600:intron 40.0%
TTACCAGCTGGGATATTGTT+GGG - chr1_4:5298767-5298786 Msa0145600:intron 40.0%
!! TTGGGCTTCACATTTGGTTT+TGG - chr1_4:5298785-5298804 Msa0145600:intron 40.0%
! TGTTATCAGGGTATTTGGTC+AGG - chr1_4:5299000-5299019 Msa0145600:CDS 40.0%
CAAGATCGAGCTCGTTAACA+TGG + chr1_4:5295760-5295779 None:intergenic 45.0%
!! GTTATTGCTGGTTTCGCCTT+TGG - chr1_4:5295803-5295822 Msa0145600:intron 45.0%
! TGGTTTCGCCTTTGGTTTCT+TGG - chr1_4:5295811-5295830 Msa0145600:intron 45.0%
AGTGCATTGGAGACACTATG+TGG - chr1_4:5296093-5296112 Msa0145600:intron 45.0%
GTGCATTGGAGACACTATGT+GGG - chr1_4:5296094-5296113 Msa0145600:intron 45.0%
ACACTATGTGGGCAAGCATT+TGG - chr1_4:5296105-5296124 Msa0145600:intron 45.0%
! TGGATTATCTTGGTGGCTAC+AGG - chr1_4:5296171-5296190 Msa0145600:intron 45.0%
! CCCTCTTTGAGAACATTAGC+GGG + chr1_4:5296819-5296838 None:intergenic 45.0%
CCCGCTAATGTTCTCAAAGA+GGG - chr1_4:5296816-5296835 Msa0145600:intron 45.0%
! CCCCTCTTTGAGAACATTAG+CGG + chr1_4:5296820-5296839 None:intergenic 45.0%
CCGCTAATGTTCTCAAAGAG+GGG - chr1_4:5296817-5296836 Msa0145600:intron 45.0%
TTCTCAAAGAGGGGACCAAA+GGG - chr1_4:5296826-5296845 Msa0145600:intron 45.0%
GCCATTATCGTGTAATGACC+TGG + chr1_4:5297312-5297331 None:intergenic 45.0%
TGGTAGGCACATTTGCTTTG+TGG - chr1_4:5297392-5297411 Msa0145600:intron 45.0%
ACAAGCACAAAGGAAAGTCC+TGG - chr1_4:5297468-5297487 Msa0145600:intron 45.0%
! GGAAAGTCCTGGTGATGTTA+TGG - chr1_4:5297479-5297498 Msa0145600:intron 45.0%
TCACCAAATCAGATGGTGCT+TGG - chr1_4:5297644-5297663 Msa0145600:intron 45.0%
ACTCCAAGCACCATCTGATT+TGG + chr1_4:5297650-5297669 None:intergenic 45.0%
AAATCAGATGGTGCTTGGAG+TGG - chr1_4:5297649-5297668 Msa0145600:intron 45.0%
GATCATGGCAACAACACCAA+TGG + chr1_4:5298442-5298461 None:intergenic 45.0%
! AACCAGACAAGACTGGTTGT+AGG + chr1_4:5298578-5298597 None:intergenic 45.0%
TGTGACTAACCAGACAAGAC+TGG + chr1_4:5298585-5298604 None:intergenic 45.0%
!! TTAGGTGTTGCTATTGGAGC+TGG - chr1_4:5298692-5298711 Msa0145600:CDS 45.0%
!!! CACATTTGGTTTTGGAGCAG+AGG - chr1_4:5298793-5298812 Msa0145600:intron 45.0%
GGTATTTGGTCAGGTCTGAT+TGG - chr1_4:5299009-5299028 Msa0145600:CDS 45.0%
ATTTGGTCAGGTCTGATTGG+AGG - chr1_4:5299012-5299031 Msa0145600:CDS 45.0%
GAGTGGAGTCGAAGAAGCTA+TGG - chr1_4:5295660-5295679 Msa0145600:intron 50.0%
TGTTAGAACGCACGGGTGTA+CGG - chr1_4:5295950-5295969 Msa0145600:intron 50.0%
TGGTTGGCAACAGTTGGGTA+TGG - chr1_4:5296068-5296087 Msa0145600:CDS 50.0%
GGTTGGCAACAGTTGGGTAT+GGG - chr1_4:5296069-5296088 Msa0145600:CDS 50.0%
! GTTGGGTATGGGAAGTGCAT+TGG - chr1_4:5296080-5296099 Msa0145600:CDS 50.0%
TGTCCAACGAGCTTCAAGAG+GGG + chr1_4:5296231-5296250 None:intergenic 50.0%
CTGTCCAACGAGCTTCAAGA+GGG + chr1_4:5296232-5296251 None:intergenic 50.0%
TCTGTCCAACGAGCTTCAAG+AGG + chr1_4:5296233-5296252 None:intergenic 50.0%
GCCCGCTAATGTTCTCAAAG+AGG - chr1_4:5296815-5296834 Msa0145600:intron 50.0%
GTTCTCAAAGAGGGGACCAA+AGG - chr1_4:5296825-5296844 Msa0145600:intron 50.0%
!! AAGGGCAAGGTGTTGCGATA+TGG + chr1_4:5296969-5296988 None:intergenic 50.0%
! GTGCTTGGAGTGGATTCTCA+TGG - chr1_4:5297659-5297678 Msa0145600:intron 50.0%
! GTGATGGCAGCTTGGAGTTT+TGG - chr1_4:5297827-5297846 Msa0145600:intron 50.0%
! GGTGGTGATAACAGGGCATT+TGG - chr1_4:5297864-5297883 Msa0145600:intron 50.0%
! AGGGCATTTGGCAAACCCTT+TGG - chr1_4:5297876-5297895 Msa0145600:intron 50.0%
CAGCATCCAATGGCACCAAA+GGG + chr1_4:5297894-5297913 None:intergenic 50.0%
ACAGCATCCAATGGCACCAA+AGG + chr1_4:5297895-5297914 None:intergenic 50.0%
TGGGATATGATGATCGCGCT+AGG - chr1_4:5298186-5298205 Msa0145600:intron 50.0%
GGGATATGATGATCGCGCTA+GGG - chr1_4:5298187-5298206 Msa0145600:intron 50.0%
TTCAAGCTATGAGGTTGCCC+AGG - chr1_4:5298503-5298522 Msa0145600:intron 50.0%
! AGCCTACAACCAGTCTTGTC+TGG - chr1_4:5298573-5298592 Msa0145600:CDS 50.0%
! GTGTTGCTATTGGAGCTGGT+TGG - chr1_4:5298696-5298715 Msa0145600:CDS 50.0%
AAGCCCAACAATATCCCAGC+TGG + chr1_4:5298773-5298792 None:intergenic 50.0%
ATGAAGCAGAAAGCCGTGTG+AGG - chr1_4:5299182-5299201 Msa0145600:CDS 50.0%
AGAAAGCCGTGTGAGGAAGT+GGG - chr1_4:5299189-5299208 Msa0145600:CDS 50.0%
!! TGTGGGCAAGCATTTGGTGC+TGG - chr1_4:5296111-5296130 Msa0145600:intron 55.0%
ACTCCCCTCTTGAAGCTCGT+TGG - chr1_4:5296225-5296244 Msa0145600:CDS 55.0%
GATGAGATCGCAGAAGCTGC+TGG - chr1_4:5296255-5296274 Msa0145600:CDS 55.0%
ATATGGCGGCTGTACCATGG+GGG + chr1_4:5296952-5296971 None:intergenic 55.0%
GATATGGCGGCTGTACCATG+GGG + chr1_4:5296953-5296972 None:intergenic 55.0%
CGATATGGCGGCTGTACCAT+GGG + chr1_4:5296954-5296973 None:intergenic 55.0%
!! GGCAAGGTGTTGCGATATGG+CGG + chr1_4:5296966-5296985 None:intergenic 55.0%
!! GGCAAACCCTTTGGTGCCAT+TGG - chr1_4:5297885-5297904 Msa0145600:intron 55.0%
CAGAAAGCCGTGTGAGGAAG+TGG - chr1_4:5299188-5299207 Msa0145600:CDS 55.0%
GAAAGCCGTGTGAGGAAGTG+GGG - chr1_4:5299190-5299209 Msa0145600:CDS 55.0%
TTCCTCCCCACTTCCTCACA+CGG + chr1_4:5299198-5299217 None:intergenic 55.0%
AACTGGTACCAGAGCCCCCA+TGG - chr1_4:5296935-5296954 Msa0145600:CDS 60.0%
GCGATATGGCGGCTGTACCA+TGG + chr1_4:5296955-5296974 None:intergenic 60.0%
CGCAACACCTTGCCCTTGCT+AGG - chr1_4:5296972-5296991 Msa0145600:intron 60.0%
AGCCGTGTGAGGAAGTGGGG+AGG - chr1_4:5299193-5299212 Msa0145600:CDS 65.0%
CGGCTGTACCATGGGGGCTC+TGG + chr1_4:5296946-5296965 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 5295560 5299237 5295560 ID=Msa0145600;Name=Msa0145600
chr1_4 mRNA 5295560 5299237 5295560 ID=Msa0145600-mRNA-1;Parent=Msa0145600;Name=Msa0145600-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0.12|0|1|1|1|9|0|487
chr1_4 exon 5298965 5299237 5298965 ID=Msa0145600-mRNA-1:exon:2237;Parent=Msa0145600-mRNA-1
chr1_4 exon 5298521 5298716 5298521 ID=Msa0145600-mRNA-1:exon:2236;Parent=Msa0145600-mRNA-1
chr1_4 exon 5297054 5297399 5297054 ID=Msa0145600-mRNA-1:exon:2235;Parent=Msa0145600-mRNA-1
chr1_4 exon 5296874 5296960 5296874 ID=Msa0145600-mRNA-1:exon:2234;Parent=Msa0145600-mRNA-1
chr1_4 exon 5296571 5296627 5296571 ID=Msa0145600-mRNA-1:exon:2233;Parent=Msa0145600-mRNA-1
chr1_4 exon 5296203 5296441 5296203 ID=Msa0145600-mRNA-1:exon:2232;Parent=Msa0145600-mRNA-1
chr1_4 exon 5295983 5296101 5295983 ID=Msa0145600-mRNA-1:exon:2231;Parent=Msa0145600-mRNA-1
chr1_4 exon 5295702 5295788 5295702 ID=Msa0145600-mRNA-1:exon:2230;Parent=Msa0145600-mRNA-1
chr1_4 exon 5295560 5295619 5295560 ID=Msa0145600-mRNA-1:exon:2229;Parent=Msa0145600-mRNA-1
chr1_4 CDS 5298965 5299237 5298965 ID=Msa0145600-mRNA-1:cds;Parent=Msa0145600-mRNA-1
chr1_4 CDS 5298521 5298716 5298521 ID=Msa0145600-mRNA-1:cds;Parent=Msa0145600-mRNA-1
chr1_4 CDS 5297054 5297399 5297054 ID=Msa0145600-mRNA-1:cds;Parent=Msa0145600-mRNA-1
chr1_4 CDS 5296874 5296960 5296874 ID=Msa0145600-mRNA-1:cds;Parent=Msa0145600-mRNA-1
chr1_4 CDS 5296571 5296627 5296571 ID=Msa0145600-mRNA-1:cds;Parent=Msa0145600-mRNA-1
chr1_4 CDS 5296203 5296441 5296203 ID=Msa0145600-mRNA-1:cds;Parent=Msa0145600-mRNA-1
chr1_4 CDS 5295983 5296101 5295983 ID=Msa0145600-mRNA-1:cds;Parent=Msa0145600-mRNA-1
chr1_4 CDS 5295702 5295788 5295702 ID=Msa0145600-mRNA-1:cds;Parent=Msa0145600-mRNA-1
chr1_4 CDS 5295560 5295619 5295560 ID=Msa0145600-mRNA-1:cds;Parent=Msa0145600-mRNA-1
Gene Sequence

>Msa0145600

ATGGCTATCGACAGTCCTCTACTAGATAACAATGTCAACTCTCCTCCTAGTGTTGTTCATCATGAGGAAGAGAAGCCACTGAAAATTGTTCACAAAGTTGGAGTGGAGTCGAAGAAGCTATGGAAAATAGCCGGTCCAACAATCTTAACTTCTTTATCTCAGTACTCGCTTGGTGCATTCACTTCTACGTTTGTTGGCCATGTTAACGAGCTCGATCTTGCAGCTTTTTCTGTTGAAAATTCTGTTATTGCTGGTTTCGCCTTTGGTTTCTTGTTGGGTATGGGAAGTGCATTGGAGACACTATGTGGGCAAGCATTTGGTGCTGGTCATATAAGAATGATGGGAATTTACATGCAAAGATCATGGATTATCTTGGTGGCTACAGGCATTTTGTTGTTACCTTTTTATATTTGGGCAACTCCCCTCTTGAAGCTCGTTGGACAGACTGATGAGATCGCAGAAGCTGCTGGCACATTTGCTTTGTGGATGATACCACAAATATTTGCATATGCTTTGAACTTTCCAATTCAAAAGTTCTTACAAGCACAAAGGAAAGTCCTGGTGATGTTATGGATATCATTAGGTGTTTTAGTGTTACATGTACCTCTTAGTTGGCTTCTAATATTGAAACTTAATTGGGGCTTAGTTGGAGCTGCAATTATTCTCAACACATCATGGTGGTTGATTGTCATTGGTCAACTTTTGTACATTTTCATCACCAAATCAGATGGTGCTTGGAGTGGATTCTCATGGCTAGCATTTGCAGATTTGTTTAATTTTTTGAAACTTTCTTTAGCTTCAGCTGTTATGTTATGCTTGGAGTTTTGGTACTTGATGATATTGGTGGTGATAACAGGGCATTTGGCAAACCCTTTGGTGCCATTGGATGCTGTATCTATTTGTATGAACATAAATGGTTGGGATATGATGATCGCGCTAGGGTTTAACGTTGCAATAAGTGTGAGAGTATCAAATGAACTTGGAGCAGGTGATTTTGAAGCTGCAAAGTTCTCAGTGATAGTGGTTTCTCTCACATCAATATCCATTGGTGTTGTTGCCATGATCATAGTACTAACAACTAGAGATTACTTTCCTCAATTGTTCACTTCAAGCTATGAGGTTGCCCAGGAAACAACTAAACTTGCTGCTTTGCTTAGTATCACTGTACTTCTAAACAGCCTACAACCAGTCTTGTCTGGTGTTGCTATTGGAGCTGGTTGGCAATCTCTTGTAGCTTACATAAATCTTGGATCCTACTATGTTGTTGGATTACCAGCTGGGATATTGTTGGGCTTCACATTTGGTTTTGGAGCAGAGGGTATTTGGTCAGGTCTGATTGGAGGCATAGCTGTGCAGACCATAATCCTTATAATTGTTACTTCATTAACCAACTGGAAAAAAGAGGCTGATGAAGCAGAAAGCCGTGTGAGGAAGTGGGGAGGAACAATTTCAATTGAGAATTAA
Protein sequence

>Msa0145600

MAIDSPLLDNNVNSPPSVVHHEEEKPLKIVHKVGVESKKLWKIAGPTILTSLSQYSLGAFTSTFVGHVNELDLAAFSVENSVIAGFAFGFLLGMGSALETLCGQAFGAGHIRMMGIYMQRSWIILVATGILLLPFYIWATPLLKLVGQTDEIAEAAGTFALWMIPQIFAYALNFPIQKFLQAQRKVLVMLWISLGVLVLHVPLSWLLILKLNWGLVGAAIILNTSWWLIVIGQLLYIFITKSDGAWSGFSWLAFADLFNFLKLSLASAVMLCLEFWYLMILVVITGHLANPLVPLDAVSICMNINGWDMMIALGFNVAISVRVSNELGAGDFEAAKFSVIVVSLTSISIGVVAMIIVLTTRDYFPQLFTSSYEVAQETTKLAALLSITVLLNSLQPVLSGVAIGAGWQSLVAYINLGSYYVVGLPAGILLGFTFGFGAEGIWSGLIGGIAVQTIILIIVTSLTNWKKEADEAESRVRKWGGTISIEN*