AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0152240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0152240 MtrunA17_Chr1g0161241 89.150 341 7 1 7 317 393 733 0.0 607
Msa0152240 MtrunA17_Chr8g0391721 71.299 331 70 3 7 312 393 723 4.88e-160 464
Msa0152240 MtrunA17_Chr1g0161961 82.927 41 7 0 7 47 54 94 9.00e-17 75.9
Msa0152240 MtrunA17_Chr1g0161961 97.143 35 1 0 208 242 97 131 2.88e-16 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0152240 AT2G01690.1 61.972 355 87 6 7 317 393 743 1.70e-136 404
Msa0152240 AT2G01690.2 61.972 355 87 6 7 317 394 744 1.84e-136 404

Find 83 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTAAAGTTTCTTATAATTT+AGG 0.091731 1_4:+14814227 Msa0152240:three_prime_UTR
TCGATCCTGCAAGATATTTA+TGG 0.136387 1_4:+14812863 Msa0152240:CDS
CAGACTAGATAATTCTCTTT+TGG 0.230548 1_4:+14811004 Msa0152240:CDS
GTAAAGTTTCTTATAATTTA+GGG 0.236641 1_4:+14814228 Msa0152240:three_prime_UTR
GGATAAATTGATTCGCTTGC+TGG 0.254134 1_4:+14812607 Msa0152240:CDS
GGATGGTGAATATTGGGTTT+TGG 0.274248 1_4:+14814083 Msa0152240:three_prime_UTR
AGGTTGGGATGGTGAATATT+GGG 0.285559 1_4:+14814077 Msa0152240:three_prime_UTR
TCAAGATCAAGGAGAAATTC+AGG 0.304942 1_4:+14813924 Msa0152240:CDS
ATAGTACACTCGATATAAAA+TGG 0.308678 1_4:-14814311 None:intergenic
CAATACCGAATATGGTGTTT+TGG 0.320973 1_4:+14810504 None:intergenic
CTTTGCTTATCTTAGATTGC+AGG 0.327847 1_4:+14812640 Msa0152240:CDS
TTAGCATTATTTCTATCTTT+CGG 0.329947 1_4:-14814276 None:intergenic
GAGGTTGGGATGGTGAATAT+TGG 0.341096 1_4:+14814076 Msa0152240:three_prime_UTR
CAAGAGATCCTCAGCACTTC+CGG 0.342233 1_4:+14810952 Msa0152240:CDS
ATTAGAAGGAGAATCTGATT+TGG 0.355199 1_4:+14811201 Msa0152240:CDS
AACTGAATAGAGAGCCTTCT+GGG 0.361056 1_4:+14813235 Msa0152240:CDS
CAGGCATCTGAGGAAGAAAT+TGG 0.364245 1_4:-14813266 None:intergenic
AATAGCCATAAATATCTTGC+AGG 0.379710 1_4:-14812868 None:intergenic
TGAAATCCTTGTGGGCTAGA+TGG 0.384041 1_4:+14814192 Msa0152240:three_prime_UTR
GAACTTTCTACAATATTAGA+AGG 0.384394 1_4:+14811187 Msa0152240:CDS
TTGGAATCAAGATGAAGCTT+GGG 0.401994 1_4:+14814102 Msa0152240:three_prime_UTR
CAACTGAATAGAGAGCCTTC+TGG 0.404102 1_4:+14813234 Msa0152240:CDS
ATATTTCTTTGTATGCCTCT+TGG 0.412645 1_4:+14812274 Msa0152240:CDS
TGGCTATTCAAAGCATTGTA+TGG 0.417465 1_4:+14812883 Msa0152240:CDS
GAATTTCTCCTTGATCTTGA+AGG 0.431307 1_4:-14813921 None:intergenic
AACTTTGAATGGGGTCGGTC+GGG 0.432159 1_4:+14814008 Msa0152240:three_prime_UTR
AAGTCTCTGCTTTGTAGCTC+AGG 0.434052 1_4:+14812320 Msa0152240:CDS
CTAATATTGTAGAAAGTTCA+CGG 0.449070 1_4:-14811183 None:intergenic
CAGTGCATATGAACTTTGAA+TGG 0.464908 1_4:+14813997 Msa0152240:three_prime_UTR
AGTGCATATGAACTTTGAAT+GGG 0.474984 1_4:+14813998 Msa0152240:three_prime_UTR
TAATGTTGTTTCTATCTATG+TGG 0.475469 1_4:+14813966 Msa0152240:three_prime_UTR
TCTTCTGATGTTGCTACCTC+AGG 0.477620 1_4:+14812906 Msa0152240:CDS
GAAAATGCAGCAGCAACATC+GGG 0.482689 1_4:+14813377 Msa0152240:CDS
AATCTAAGATAAGCAAAGAT+TGG 0.483573 1_4:-14812635 None:intergenic
AAGTGCTGTGATTCAATCTC+TGG 0.486778 1_4:+14812547 Msa0152240:CDS
CAGAGACTTATAATTGCCAT+TGG 0.488893 1_4:-14812306 None:intergenic
AGCGAATCAATTTATCCAGT+TGG 0.488979 1_4:-14812601 None:intergenic
TTTCTATCTTTCGGAGGCAT+AGG 0.494223 1_4:-14814267 None:intergenic
TTTCTTCCTCAGATGCCTGA+TGG 0.494336 1_4:+14813270 Msa0152240:CDS
GAAATCCTTGTGGGCTAGAT+GGG 0.501775 1_4:+14814193 Msa0152240:three_prime_UTR
TTTGGAATCAAGATGAAGCT+TGG 0.512873 1_4:+14814101 Msa0152240:three_prime_UTR
CAATCACTGGTAAATCCTGC+CGG 0.515153 1_4:+14812240 Msa0152240:CDS
ATGTCCCTCCTTCAAGATCA+AGG 0.516089 1_4:+14813913 Msa0152240:CDS
GAACTTTGAATGGGGTCGGT+CGG 0.519465 1_4:+14814007 Msa0152240:three_prime_UTR
AGAAATATACAAATCCTTCC+CGG 0.521247 1_4:-14812259 None:intergenic
CTAGTCTGAAATTGTGCACA+AGG 0.521586 1_4:-14810989 None:intergenic
CTATCTTTCGGAGGCATAGG+AGG 0.522475 1_4:-14814264 None:intergenic
CAAGATCAAGGAGAAATTCA+GGG 0.537258 1_4:+14813925 Msa0152240:CDS
GATTCAATCTCTGGAGGAGG+AGG 0.539665 1_4:+14812556 Msa0152240:CDS
TTCTCCTTGATCTTGAAGGA+GGG 0.541578 1_4:-14813917 None:intergenic
TTTCTCCTTGATCTTGAAGG+AGG 0.541709 1_4:-14813918 None:intergenic
TTTATTTATAGAGGTTGGGA+TGG 0.549584 1_4:+14814066 Msa0152240:three_prime_UTR
TTTGTTCCATCAGGCATCTG+AGG 0.550164 1_4:-14813276 None:intergenic
CTCTTGGTGTCATTCTCCAA+TGG 0.560351 1_4:+14812290 Msa0152240:CDS
ATATACAAATCCTTCCCGGC+AGG 0.561385 1_4:-14812255 None:intergenic
AGAAAATGCAGCAGCAACAT+CGG 0.563908 1_4:+14813376 Msa0152240:CDS
GATGGTGATGCAACTGAAGA+TGG 0.565704 1_4:+14813303 Msa0152240:CDS
AAGTTCACGGTAGACTCTCT+CGG 0.573576 1_4:-14811170 None:intergenic
CAAGGAAAACGACAAGATGC+CGG 0.577859 1_4:-14810971 None:intergenic
GATGCCTGATGGAACAAAAG+AGG 0.583988 1_4:+14813281 Msa0152240:CDS
TGTGATTCAATCTCTGGAGG+AGG 0.585756 1_4:+14812553 Msa0152240:CDS
ACTGGTAAATCCTGCCGGGA+AGG 0.587571 1_4:+14812245 Msa0152240:CDS
TGGAATCAAGATGAAGCTTG+GGG 0.588271 1_4:+14814103 Msa0152240:three_prime_UTR
GAGCATCACGTTCTTCTACA+AGG 0.590591 1_4:-14814148 None:intergenic
AATCACTGGTAAATCCTGCC+GGG 0.591032 1_4:+14812241 Msa0152240:CDS
ATATGAACTTTGAATGGGGT+CGG 0.602899 1_4:+14814003 Msa0152240:three_prime_UTR
TGCTGTGATTCAATCTCTGG+AGG 0.606142 1_4:+14812550 Msa0152240:CDS
TATGCAGGAGGCACAACAAG+AGG 0.609276 1_4:+14813857 Msa0152240:intron
GCATTATTTCTATCTTTCGG+AGG 0.615467 1_4:-14814273 None:intergenic
AATATCCCATCTAGCCCACA+AGG 0.627127 1_4:-14814198 None:intergenic
AATTGGTAATAATCCCCAGA+AGG 0.629567 1_4:-14813249 None:intergenic
ACAAGATGCCGGAAGTGCTG+AGG 0.637595 1_4:-14810960 None:intergenic
AGAAGAACGTGATGCTCACG+TGG 0.646393 1_4:+14814154 Msa0152240:three_prime_UTR
GAATCACAGCACTTGCATGT+CGG 0.655650 1_4:-14812538 None:intergenic
GTGATGCAACTGAAGATGGT+GGG 0.660086 1_4:+14813307 Msa0152240:CDS
GGTGATGCAACTGAAGATGG+TGG 0.664040 1_4:+14813306 Msa0152240:CDS
CATTGGAGAATGACACCAAG+AGG 0.667584 1_4:-14812289 None:intergenic
CCTGATGGAACAAAAGAGGA+TGG 0.675641 1_4:+14813285 Msa0152240:CDS
GGAATCAAGATGAAGCTTGG+GGG 0.676324 1_4:+14814104 Msa0152240:three_prime_UTR
ACTGAATAGAGAGCCTTCTG+GGG 0.693100 1_4:+14813236 Msa0152240:CDS
CGGCATTCAAAAGCACACAA+AGG 0.693511 1_4:-14811150 None:intergenic
GTGCATATGAACTTTGAATG+GGG 0.701952 1_4:+14813999 Msa0152240:three_prime_UTR
GCACGATTGAAAGAATACGA+TGG 0.736541 1_4:-14813210 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAAAAAATTTAAAATA+GGG - chr1_4:14811535-14811554 None:intergenic 0.0%
!!! ATTTTTAAAAAATTTAAAAT+AGG - chr1_4:14811536-14811555 None:intergenic 0.0%
!!! TGAATTTTTTAATATCAAAT+AGG + chr1_4:14810893-14810912 Msa0152240:intron 10.0%
!!! ATTTTTTAAAACATTAATGT+TGG - chr1_4:14811455-14811474 None:intergenic 10.0%
!!! GAAAATCATAATATTTTTTA+GGG + chr1_4:14811776-14811795 Msa0152240:intron 10.0%
!! AAATTTAATTACATAAATAG+GGG - chr1_4:14812496-14812515 None:intergenic 10.0%
!! CAAATTTAATTACATAAATA+GGG - chr1_4:14812497-14812516 None:intergenic 10.0%
!!! AGAATCATATTTTATAATTA+GGG - chr1_4:14812732-14812751 None:intergenic 10.0%
!!! AAGAATCATATTTTATAATT+AGG - chr1_4:14812733-14812752 None:intergenic 10.0%
!! ATTATAAAATATGATTCTTA+TGG + chr1_4:14812734-14812753 Msa0152240:intron 10.0%
!!! TAAAATGAAGTTTTAATGAA+AGG - chr1_4:14811091-14811110 None:intergenic 15.0%
!!! CATTTTATACAATTAAATCT+AGG + chr1_4:14811104-14811123 Msa0152240:intron 15.0%
!! CAAACAAAAATATTATATTG+AGG + chr1_4:14811663-14811682 Msa0152240:intron 15.0%
!!! GGAAAATCATAATATTTTTT+AGG + chr1_4:14811775-14811794 Msa0152240:intron 15.0%
!! CCAAATTTAATTACATAAAT+AGG - chr1_4:14812498-14812517 None:intergenic 15.0%
!! CCTATTTATGTAATTAAATT+TGG + chr1_4:14812495-14812514 Msa0152240:intron 15.0%
!! TTATTATAGTATTAGTTATG+TGG + chr1_4:14812769-14812788 Msa0152240:intron 15.0%
!!! ATATTTATCTTTTTTGAACA+AGG - chr1_4:14813646-14813665 None:intergenic 15.0%
!! AGTAAAGTTTCTTATAATTT+AGG + chr1_4:14814227-14814246 Msa0152240:three_prime_UTR 15.0%
!! GTAAAGTTTCTTATAATTTA+GGG + chr1_4:14814228-14814247 Msa0152240:three_prime_UTR 15.0%
!! TTAAACAATATAGCATACAA+GGG - chr1_4:14810827-14810846 None:intergenic 20.0%
!! ATTAAACAATATAGCATACA+AGG - chr1_4:14810828-14810847 None:intergenic 20.0%
!! TATGCTATATTGTTTAATTG+TGG + chr1_4:14810830-14810849 Msa0152240:intron 20.0%
!! ACATAACTATTTCGATTAAT+GGG + chr1_4:14811345-14811364 Msa0152240:intron 20.0%
!! TAAACAAAATAACTTTATGG+AGG - chr1_4:14811563-14811582 None:intergenic 20.0%
!! TGGTAAACAAAATAACTTTA+TGG - chr1_4:14811566-14811585 None:intergenic 20.0%
!! TAGATAATAGTGAAATGTAA+TGG + chr1_4:14811741-14811760 Msa0152240:intron 20.0%
!! CATGCACATTAAATTAAAAA+TGG - chr1_4:14812683-14812702 None:intergenic 20.0%
!! TATCTTAAAAGTCTTACAAT+GGG - chr1_4:14813615-14813634 None:intergenic 20.0%
!! TTATCTTAAAAGTCTTACAA+TGG - chr1_4:14813616-14813635 None:intergenic 20.0%
!!! TTAGCATTATTTCTATCTTT+CGG - chr1_4:14814279-14814298 None:intergenic 20.0%
!!! ATTTTTCTTTAAAGCCACAT+TGG + chr1_4:14810607-14810626 Msa0152240:intron 25.0%
! TAAGAAGTCATTCCTAATAT+GGG - chr1_4:14810639-14810658 None:intergenic 25.0%
! AATTCATGTGAATAATTTCC+AGG - chr1_4:14810859-14810878 None:intergenic 25.0%
! CTAATATTGTAGAAAGTTCA+CGG - chr1_4:14811186-14811205 None:intergenic 25.0%
! GAACTTTCTACAATATTAGA+AGG + chr1_4:14811187-14811206 Msa0152240:CDS 25.0%
!! TTTGGATTTTGCATCAATTA+TGG + chr1_4:14811219-14811238 Msa0152240:CDS 25.0%
! TAATAATATGCTGTGTTATG+TGG + chr1_4:14811314-14811333 Msa0152240:intron 25.0%
! GACATAACTATTTCGATTAA+TGG + chr1_4:14811344-14811363 Msa0152240:intron 25.0%
!!! ATTATGGAGTACATGTTTTT+TGG + chr1_4:14811475-14811494 Msa0152240:intron 25.0%
!! GGAAAATAGCAAAATTTTGA+GGG - chr1_4:14811502-14811521 None:intergenic 25.0%
!!! ATTATATTGAGGACTTGTTA+AGG + chr1_4:14811674-14811693 Msa0152240:intron 25.0%
!! AGCACCATTAATTATGTTTT+CGG - chr1_4:14811721-14811740 None:intergenic 25.0%
! ATAATAGTGAAATGTAATGG+AGG + chr1_4:14811744-14811763 Msa0152240:intron 25.0%
!!! AAGTTTGCTAAATATGTTGT+TGG - chr1_4:14811807-14811826 None:intergenic 25.0%
! AAACTTAAATTGTACTTCGA+TGG + chr1_4:14811821-14811840 Msa0152240:intron 25.0%
! CAAAAAGATAATATTGCCAA+TGG - chr1_4:14812035-14812054 None:intergenic 25.0%
! GGTATATATCATCTATAACT+TGG + chr1_4:14812341-14812360 Msa0152240:intron 25.0%
!!! AGTAATCAATTTTTTTTCCG+TGG + chr1_4:14812439-14812458 Msa0152240:intron 25.0%
!! GGATATAAATGTGAAGTTTT+TGG + chr1_4:14812577-14812596 Msa0152240:CDS 25.0%
!! AATCTAAGATAAGCAAAGAT+TGG - chr1_4:14812638-14812657 None:intergenic 25.0%
!! ATTTTATAATTAGGGACGAA+TGG - chr1_4:14812724-14812743 None:intergenic 25.0%
!!! TATTTTTTAAACTTCAGTGC+TGG + chr1_4:14813010-14813029 Msa0152240:intron 25.0%
!!! ACGAGTTTTTAGTATCTTAA+AGG - chr1_4:14813178-14813197 None:intergenic 25.0%
!!! TAATGTTGTTTCTATCTATG+TGG + chr1_4:14813966-14813985 Msa0152240:three_prime_UTR 25.0%
!!! TCGTTTTATTTATAGAGGTT+GGG + chr1_4:14814062-14814081 Msa0152240:three_prime_UTR 25.0%
!!! TATTTTTGTTGAAATCCTTG+TGG + chr1_4:14814183-14814202 Msa0152240:three_prime_UTR 25.0%
!! ATTTTTGTTGAAATCCTTGT+GGG + chr1_4:14814184-14814203 Msa0152240:three_prime_UTR 25.0%
! AAGAACAAGTTGAAATCCTA+TGG - chr1_4:14810577-14810596 None:intergenic 30.0%
AAGAAGTCATTCCTAATATG+GGG - chr1_4:14810638-14810657 None:intergenic 30.0%
GTAAGAAGTCATTCCTAATA+TGG - chr1_4:14810640-14810659 None:intergenic 30.0%
AATAACAAACCAGAGACTAA+TGG - chr1_4:14810753-14810772 None:intergenic 30.0%
ATTGTTTAATTGTGGAAACC+TGG + chr1_4:14810838-14810857 Msa0152240:intron 30.0%
! CAGACTAGATAATTCTCTTT+TGG + chr1_4:14811004-14811023 Msa0152240:CDS 30.0%
ACTAGAAGAATACATATGAC+CGG - chr1_4:14811053-14811072 None:intergenic 30.0%
TATACAATTAAATCTAGGCG+TGG + chr1_4:14811109-14811128 Msa0152240:intron 30.0%
ATTAGAAGGAGAATCTGATT+TGG + chr1_4:14811201-14811220 Msa0152240:CDS 30.0%
! TTTTGCATCAATTATGGTTC+AGG + chr1_4:14811225-14811244 Msa0152240:CDS 30.0%
! AACTATTTCGATTAATGGGT+AGG + chr1_4:14811349-14811368 Msa0152240:intron 30.0%
! GGGAAAATAGCAAAATTTTG+AGG - chr1_4:14811503-14811522 None:intergenic 30.0%
TTAAAATAGGGAGAGCAAAT+GGG - chr1_4:14811523-14811542 None:intergenic 30.0%
! TTTAAAATAGGGAGAGCAAA+TGG - chr1_4:14811524-14811543 None:intergenic 30.0%
CACTTAACAATTGAATGCAA+TGG - chr1_4:14811610-14811629 None:intergenic 30.0%
GAGACCGAAAACATAATTAA+TGG + chr1_4:14811714-14811733 Msa0152240:intron 30.0%
!! AAATGTAATGGAGGTGTTAA+TGG + chr1_4:14811753-14811772 Msa0152240:intron 30.0%
!! AATGTAATGGAGGTGTTAAT+GGG + chr1_4:14811754-14811773 Msa0152240:intron 30.0%
AAATTGATTATAGCCTACGA+TGG - chr1_4:14811933-14811952 None:intergenic 30.0%
CCCAAAAACATCTAAAGAAA+GGG - chr1_4:14811995-14812014 None:intergenic 30.0%
! CCCTTTCTTTAGATGTTTTT+GGG + chr1_4:14811992-14812011 Msa0152240:intron 30.0%
!! CCTTTCTTTAGATGTTTTTG+GGG + chr1_4:14811993-14812012 Msa0152240:intron 30.0%
!! TCCCTTTGAAAAAACTTTTC+AGG + chr1_4:14812158-14812177 Msa0152240:intron 30.0%
!!! GCCTGAAAAGTTTTTTCAAA+GGG - chr1_4:14812162-14812181 None:intergenic 30.0%
!!! AGCCTGAAAAGTTTTTTCAA+AGG - chr1_4:14812163-14812182 None:intergenic 30.0%
!!! AGATCTTTTAAAGCAATCAC+TGG + chr1_4:14812227-14812246 Msa0152240:CDS 30.0%
AGAAATATACAAATCCTTCC+CGG - chr1_4:14812262-14812281 None:intergenic 30.0%
ATATTTCTTTGTATGCCTCT+TGG + chr1_4:14812274-14812293 Msa0152240:CDS 30.0%
GGAGAAAGAAAGACTTAAAA+TGG - chr1_4:14812383-14812402 None:intergenic 30.0%
!! GATCTAGCTTTAGCTTTATT+TGG + chr1_4:14812461-14812480 Msa0152240:intron 30.0%
AGTATTAGTTATGTGGCTAA+AGG + chr1_4:14812776-14812795 Msa0152240:intron 30.0%
AATAGCCATAAATATCTTGC+AGG - chr1_4:14812871-14812890 None:intergenic 30.0%
!! TTTTTAATTGCAGATACCTG+AGG - chr1_4:14812925-14812944 None:intergenic 30.0%
TGTGTTTCAACATCCTTTAT+TGG + chr1_4:14812980-14812999 Msa0152240:intron 30.0%
TTTCAACATCCTTTATTGGT+AGG + chr1_4:14812984-14813003 Msa0152240:intron 30.0%
AAAATACCTCCTACCAATAA+AGG - chr1_4:14812996-14813015 None:intergenic 30.0%
TTTATCCACTTCATTACCAA+AGG + chr1_4:14813429-14813448 Msa0152240:intron 30.0%
TTTGTCCACTACATTATCAA+AGG + chr1_4:14813465-14813484 Msa0152240:intron 30.0%
!! GTGATTTGTGATTGATTTGA+CGG - chr1_4:14813593-14813612 None:intergenic 30.0%
GCAAAAATTATTTACCCTGT+CGG + chr1_4:14813716-14813735 Msa0152240:intron 30.0%
! TCATTGTTTTCACTATATGC+AGG + chr1_4:14813842-14813861 Msa0152240:intron 30.0%
AGTGCATATGAACTTTGAAT+GGG + chr1_4:14813998-14814017 Msa0152240:three_prime_UTR 30.0%
!!! GTAGCTCGTTTTATTTATAG+AGG + chr1_4:14814057-14814076 Msa0152240:three_prime_UTR 30.0%
!!! CTCGTTTTATTTATAGAGGT+TGG + chr1_4:14814061-14814080 Msa0152240:three_prime_UTR 30.0%
TTTATTTATAGAGGTTGGGA+TGG + chr1_4:14814066-14814085 Msa0152240:three_prime_UTR 30.0%
! TATTTCTGATCACAAGCCAT+AGG + chr1_4:14810558-14810577 Msa0152240:intron 35.0%
AGAAGTCATTCCTAATATGG+GGG - chr1_4:14810637-14810656 None:intergenic 35.0%
! TCTTGATTACCATTAGTCTC+TGG + chr1_4:14810741-14810760 Msa0152240:intron 35.0%
AATATGCTGTGTTATGTGGA+TGG + chr1_4:14811318-14811337 Msa0152240:intron 35.0%
! TTCGATTAATGGGTAGGATT+TGG + chr1_4:14811355-14811374 Msa0152240:intron 35.0%
! CACAATCAGCTAACTTTTCA+AGG + chr1_4:14811398-14811417 Msa0152240:intron 35.0%
! TTCAAGGTTACTGTAGCTTT+TGG + chr1_4:14811414-14811433 Msa0152240:intron 35.0%
AAATTGTACTTCGATGGAGT+TGG + chr1_4:14811827-14811846 Msa0152240:intron 35.0%
! GCCCTTTCTTTAGATGTTTT+TGG + chr1_4:14811991-14812010 Msa0152240:intron 35.0%
CCCCAAAAACATCTAAAGAA+AGG - chr1_4:14811996-14812015 None:intergenic 35.0%
TCGAACAATGAAGCAGTTAA+AGG - chr1_4:14812061-14812080 None:intergenic 35.0%
GAAATTCAGACAATCCTATG+TGG + chr1_4:14812105-14812124 Msa0152240:intron 35.0%
TGAATACTGTTTCACCACAT+AGG - chr1_4:14812122-14812141 None:intergenic 35.0%
CAGAGACTTATAATTGCCAT+TGG - chr1_4:14812309-14812328 None:intergenic 35.0%
TAAAGCTAAAGCTAGATCCA+CGG - chr1_4:14812459-14812478 None:intergenic 35.0%
AGCGAATCAATTTATCCAGT+TGG - chr1_4:14812604-14812623 None:intergenic 35.0%
CTTTGCTTATCTTAGATTGC+AGG + chr1_4:14812640-14812659 Msa0152240:CDS 35.0%
TCGATCCTGCAAGATATTTA+TGG + chr1_4:14812863-14812882 Msa0152240:CDS 35.0%
!! TGGCTATTCAAAGCATTGTA+TGG + chr1_4:14812883-14812902 Msa0152240:CDS 35.0%
!!! TGGTGCGAGTTTTGAATTTA+CGG + chr1_4:14813098-14813117 Msa0152240:intron 35.0%
! AATTGGTAATAATCCCCAGA+AGG - chr1_4:14813252-14813271 None:intergenic 35.0%
!! ATAATGTAGTGGACAAACTG+AGG - chr1_4:14813462-14813481 None:intergenic 35.0%
!! AGCAACCTTTGATAATGTAG+TGG - chr1_4:14813473-14813492 None:intergenic 35.0%
CTACATTATCAAAGGTTGCT+AGG + chr1_4:14813473-14813492 Msa0152240:intron 35.0%
! TTAATTCATATGCACCGACA+GGG - chr1_4:14813733-14813752 None:intergenic 35.0%
! TTTAATTCATATGCACCGAC+AGG - chr1_4:14813734-14813753 None:intergenic 35.0%
! TTGTTTTCACTATATGCAGG+AGG + chr1_4:14813845-14813864 Msa0152240:intron 35.0%
! TCAATAGACTGAGTTTTCTG+TGG - chr1_4:14813888-14813907 None:intergenic 35.0%
GAATTTCTCCTTGATCTTGA+AGG - chr1_4:14813924-14813943 None:intergenic 35.0%
TCAAGATCAAGGAGAAATTC+AGG + chr1_4:14813924-14813943 Msa0152240:CDS 35.0%
CAAGATCAAGGAGAAATTCA+GGG + chr1_4:14813925-14813944 Msa0152240:CDS 35.0%
CAGTGCATATGAACTTTGAA+TGG + chr1_4:14813997-14814016 Msa0152240:three_prime_UTR 35.0%
GTGCATATGAACTTTGAATG+GGG + chr1_4:14813999-14814018 Msa0152240:three_prime_UTR 35.0%
ATATGAACTTTGAATGGGGT+CGG + chr1_4:14814003-14814022 Msa0152240:three_prime_UTR 35.0%
! TTTGGAATCAAGATGAAGCT+TGG + chr1_4:14814101-14814120 Msa0152240:three_prime_UTR 35.0%
TTGGAATCAAGATGAAGCTT+GGG + chr1_4:14814102-14814121 Msa0152240:three_prime_UTR 35.0%
!! GCATTATTTCTATCTTTCGG+AGG - chr1_4:14814276-14814295 None:intergenic 35.0%
! CTAGTCTGAAATTGTGCACA+AGG - chr1_4:14810992-14811011 None:intergenic 40.0%
!! GTAAGCTGTTTTCCTTCATC+CGG + chr1_4:14811031-14811050 Msa0152240:intron 40.0%
GAATACATATGACCGGATGA+AGG - chr1_4:14811046-14811065 None:intergenic 40.0%
! GCAGCTATTTGTTTAGCTCA+GGG - chr1_4:14811265-14811284 None:intergenic 40.0%
! TGCAGCTATTTGTTTAGCTC+AGG - chr1_4:14811266-14811285 None:intergenic 40.0%
GTACTAACGAAGAGATCGTT+TGG - chr1_4:14811586-14811605 None:intergenic 40.0%
TCAATTGTTAAGTGCACACG+TGG + chr1_4:14811616-14811635 Msa0152240:intron 40.0%
GTTGACCTAAAAATGCTGCA+TGG + chr1_4:14811903-14811922 Msa0152240:intron 40.0%
TGATTATAGCCTACGATGGA+GGG - chr1_4:14811929-14811948 None:intergenic 40.0%
TTGATTATAGCCTACGATGG+AGG - chr1_4:14811930-14811949 None:intergenic 40.0%
! GCACAAAGCATTAACTTCTG+TGG - chr1_4:14811963-14811982 None:intergenic 40.0%
AAGTTAATGCTTTGTGCTCC+AGG + chr1_4:14811966-14811985 Msa0152240:intron 40.0%
!!! TGTTTTTGGGGTAACAGTTC+TGG + chr1_4:14812005-14812024 Msa0152240:intron 40.0%
!!! GTTTTTGGGGTAACAGTTCT+GGG + chr1_4:14812006-14812025 Msa0152240:intron 40.0%
! AAGTGCTGTGATTCAATCTC+TGG + chr1_4:14812547-14812566 Msa0152240:CDS 40.0%
!!! TGTGAAGTTTTTGGTCCAAC+TGG + chr1_4:14812586-14812605 Msa0152240:CDS 40.0%
!! GGATAAATTGATTCGCTTGC+TGG + chr1_4:14812607-14812626 Msa0152240:CDS 40.0%
CAACATCCTTTATTGGTAGG+AGG + chr1_4:14812987-14813006 Msa0152240:intron 40.0%
TGAACGACGACGTATATACT+AGG - chr1_4:14813070-14813089 None:intergenic 40.0%
GCACGATTGAAAGAATACGA+TGG - chr1_4:14813213-14813232 None:intergenic 40.0%
AACTGAATAGAGAGCCTTCT+GGG + chr1_4:14813235-14813254 Msa0152240:CDS 40.0%
AGAAAATGCAGCAGCAACAT+CGG + chr1_4:14813376-14813395 Msa0152240:CDS 40.0%
!! AGCAACCTTTGGTAATGAAG+TGG - chr1_4:14813437-14813456 None:intergenic 40.0%
CGATGATTGTGATTGACTGT+TGG + chr1_4:14813692-14813711 Msa0152240:intron 40.0%
TTCTCCTTGATCTTGAAGGA+GGG - chr1_4:14813920-14813939 None:intergenic 40.0%
TTTCTCCTTGATCTTGAAGG+AGG - chr1_4:14813921-14813940 None:intergenic 40.0%
AGGTTGGGATGGTGAATATT+GGG + chr1_4:14814077-14814096 Msa0152240:three_prime_UTR 40.0%
! GGATGGTGAATATTGGGTTT+TGG + chr1_4:14814083-14814102 Msa0152240:three_prime_UTR 40.0%
TGGAATCAAGATGAAGCTTG+GGG + chr1_4:14814103-14814122 Msa0152240:three_prime_UTR 40.0%
! TTTCTATCTTTCGGAGGCAT+AGG - chr1_4:14814270-14814289 None:intergenic 40.0%
CAAGGAAAACGACAAGATGC+CGG - chr1_4:14810974-14810993 None:intergenic 45.0%
! CGGCATTCAAAAGCACACAA+AGG - chr1_4:14811153-14811172 None:intergenic 45.0%
AAGTTCACGGTAGACTCTCT+CGG - chr1_4:14811173-14811192 None:intergenic 45.0%
GTGCAAGATTGCAATGCTCA+AGG + chr1_4:14811852-14811871 Msa0152240:intron 45.0%
! ATGCTCAAGGCGCTATTTGA+TGG + chr1_4:14811865-14811884 Msa0152240:intron 45.0%
!! TAACAGTTCTGGGACACCAT+TGG + chr1_4:14812016-14812035 Msa0152240:intron 45.0%
CAATCACTGGTAAATCCTGC+CGG + chr1_4:14812240-14812259 Msa0152240:CDS 45.0%
AATCACTGGTAAATCCTGCC+GGG + chr1_4:14812241-14812260 Msa0152240:CDS 45.0%
ATATACAAATCCTTCCCGGC+AGG - chr1_4:14812258-14812277 None:intergenic 45.0%
CATTGGAGAATGACACCAAG+AGG - chr1_4:14812292-14812311 None:intergenic 45.0%
!! CTCTTGGTGTCATTCTCCAA+TGG + chr1_4:14812290-14812309 Msa0152240:CDS 45.0%
AAGTCTCTGCTTTGTAGCTC+AGG + chr1_4:14812320-14812339 Msa0152240:CDS 45.0%
TGTGTGTCCAATTGCACAGT+GGG - chr1_4:14812408-14812427 None:intergenic 45.0%
GAATCACAGCACTTGCATGT+CGG - chr1_4:14812541-14812560 None:intergenic 45.0%
TGCTGTGATTCAATCTCTGG+AGG + chr1_4:14812550-14812569 Msa0152240:CDS 45.0%
! TGTGATTCAATCTCTGGAGG+AGG + chr1_4:14812553-14812572 Msa0152240:CDS 45.0%
TCTTCTGATGTTGCTACCTC+AGG + chr1_4:14812906-14812925 Msa0152240:CDS 45.0%
CAACTGAATAGAGAGCCTTC+TGG + chr1_4:14813234-14813253 Msa0152240:CDS 45.0%
ACTGAATAGAGAGCCTTCTG+GGG + chr1_4:14813236-14813255 Msa0152240:CDS 45.0%
CAGGCATCTGAGGAAGAAAT+TGG - chr1_4:14813269-14813288 None:intergenic 45.0%
TTTCTTCCTCAGATGCCTGA+TGG + chr1_4:14813270-14813289 Msa0152240:CDS 45.0%
TTTGTTCCATCAGGCATCTG+AGG - chr1_4:14813279-14813298 None:intergenic 45.0%
GATGCCTGATGGAACAAAAG+AGG + chr1_4:14813281-14813300 Msa0152240:CDS 45.0%
! CCATCCTCTTTTGTTCCATC+AGG - chr1_4:14813288-14813307 None:intergenic 45.0%
CCTGATGGAACAAAAGAGGA+TGG + chr1_4:14813285-14813304 Msa0152240:CDS 45.0%
! GATGGTGATGCAACTGAAGA+TGG + chr1_4:14813303-14813322 Msa0152240:CDS 45.0%
GTGATGCAACTGAAGATGGT+GGG + chr1_4:14813307-14813326 Msa0152240:CDS 45.0%
GAAAATGCAGCAGCAACATC+GGG + chr1_4:14813377-14813396 Msa0152240:CDS 45.0%
AAACTGAGGCTAGCAACCTT+TGG - chr1_4:14813448-14813467 None:intergenic 45.0%
AGGCTCTTCATTCTGCTTGT+TGG + chr1_4:14813493-14813512 Msa0152240:intron 45.0%
ATGTCCCTCCTTCAAGATCA+AGG + chr1_4:14813913-14813932 Msa0152240:CDS 45.0%
GAGGTTGGGATGGTGAATAT+TGG + chr1_4:14814076-14814095 Msa0152240:three_prime_UTR 45.0%
GGAATCAAGATGAAGCTTGG+GGG + chr1_4:14814104-14814123 Msa0152240:three_prime_UTR 45.0%
! ATGAAGCTTGGGGGTTTTCT+GGG + chr1_4:14814113-14814132 Msa0152240:three_prime_UTR 45.0%
GAGCATCACGTTCTTCTACA+AGG - chr1_4:14814151-14814170 None:intergenic 45.0%
!! TGAAATCCTTGTGGGCTAGA+TGG + chr1_4:14814192-14814211 Msa0152240:three_prime_UTR 45.0%
!! GAAATCCTTGTGGGCTAGAT+GGG + chr1_4:14814193-14814212 Msa0152240:three_prime_UTR 45.0%
AATATCCCATCTAGCCCACA+AGG - chr1_4:14814201-14814220 None:intergenic 45.0%
!!! TAATGTTTTAAAAAATATTA+TGG + chr1_4:14811459-14811478 Msa0152240:intron 5.0%
AATATGGGGGTGAGCCAATG+TGG - chr1_4:14810624-14810643 None:intergenic 50.0%
!! CATTGGCTCACCCCCATATT+AGG + chr1_4:14810624-14810643 Msa0152240:intron 50.0%
CAAGAGATCCTCAGCACTTC+CGG + chr1_4:14810952-14810971 Msa0152240:CDS 50.0%
! GAGGGCCATGCAGCATTTTT+AGG - chr1_4:14811911-14811930 None:intergenic 50.0%
CATCTAAAGAAAGGGCAGCC+TGG - chr1_4:14811987-14812006 None:intergenic 50.0%
GTGTGTGTCCAATTGCACAG+TGG - chr1_4:14812409-14812428 None:intergenic 50.0%
GCATGTCGGTAAGTCTGTAG+TGG - chr1_4:14812527-14812546 None:intergenic 50.0%
! GATTCAATCTCTGGAGGAGG+AGG + chr1_4:14812556-14812575 Msa0152240:CDS 50.0%
CGTCGTCGTTCAGAATCTAC+TGG + chr1_4:14813078-14813097 Msa0152240:intron 50.0%
AAAGGCTGCACTTTGCTGCA+TGG - chr1_4:14813160-14813179 None:intergenic 50.0%
GGTGATGCAACTGAAGATGG+TGG + chr1_4:14813306-14813325 Msa0152240:CDS 50.0%
! TATGCAGGAGGCACAACAAG+AGG + chr1_4:14813857-14813876 Msa0152240:intron 50.0%
GAACTTTGAATGGGGTCGGT+CGG + chr1_4:14814007-14814026 Msa0152240:three_prime_UTR 50.0%
AACTTTGAATGGGGTCGGTC+GGG + chr1_4:14814008-14814027 Msa0152240:three_prime_UTR 50.0%
! GATGAAGCTTGGGGGTTTTC+TGG + chr1_4:14814112-14814131 Msa0152240:three_prime_UTR 50.0%
AGAAGAACGTGATGCTCACG+TGG + chr1_4:14814154-14814173 Msa0152240:three_prime_UTR 50.0%
! CTATCTTTCGGAGGCATAGG+AGG - chr1_4:14814267-14814286 None:intergenic 50.0%
!!! GGTGTTTTGGAACGTGGAGC+AGG + chr1_4:14810517-14810536 Msa0152240:CDS 55.0%
!! ACAAGATGCCGGAAGTGCTG+AGG - chr1_4:14810963-14810982 None:intergenic 55.0%
ACTGGTAAATCCTGCCGGGA+AGG + chr1_4:14812245-14812264 Msa0152240:CDS 55.0%
TCTCCAGCCCACTGTGCAAT+TGG + chr1_4:14812398-14812417 Msa0152240:intron 55.0%
TGTCCAATTGCACAGTGGGC+TGG - chr1_4:14812404-14812423 None:intergenic 55.0%
TGGAACGTGGAGCAGGTGAG+TGG + chr1_4:14810524-14810543 Msa0152240:intron 60.0%
GTGGAGCAGGTGAGTGGCTT+GGG + chr1_4:14810530-14810549 Msa0152240:intron 60.0%
CGTGGAGCAGGTGAGTGGCT+TGG + chr1_4:14810529-14810548 Msa0152240:intron 65.0%
GCTGCATGGCCCTCCATCGT+AGG + chr1_4:14811917-14811936 Msa0152240:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 14810515 14814320 14810515 ID=Msa0152240;Name=Msa0152240
chr1_4 mRNA 14810515 14814320 14810515 ID=Msa0152240-mRNA-1;Parent=Msa0152240;Name=Msa0152240-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0.57|0.5|0.87|1|1|8|358|317
chr1_4 exon 14810515 14810538 14810515 ID=Msa0152240-mRNA-1:exon:5814;Parent=Msa0152240-mRNA-1
chr1_4 exon 14810915 14811030 14810915 ID=Msa0152240-mRNA-1:exon:5815;Parent=Msa0152240-mRNA-1
chr1_4 exon 14811126 14811246 14811126 ID=Msa0152240-mRNA-1:exon:5816;Parent=Msa0152240-mRNA-1
chr1_4 exon 14812180 14812341 14812180 ID=Msa0152240-mRNA-1:exon:5817;Parent=Msa0152240-mRNA-1
chr1_4 exon 14812533 14812661 14812533 ID=Msa0152240-mRNA-1:exon:5818;Parent=Msa0152240-mRNA-1
chr1_4 exon 14812859 14812927 14812859 ID=Msa0152240-mRNA-1:exon:5819;Parent=Msa0152240-mRNA-1
chr1_4 exon 14813165 14813398 14813165 ID=Msa0152240-mRNA-1:exon:5820;Parent=Msa0152240-mRNA-1
chr1_4 exon 14813864 14814320 14813864 ID=Msa0152240-mRNA-1:exon:5821;Parent=Msa0152240-mRNA-1
chr1_4 CDS 14810515 14810538 14810515 ID=Msa0152240-mRNA-1:cds;Parent=Msa0152240-mRNA-1
chr1_4 CDS 14810915 14811030 14810915 ID=Msa0152240-mRNA-1:cds;Parent=Msa0152240-mRNA-1
chr1_4 CDS 14811126 14811246 14811126 ID=Msa0152240-mRNA-1:cds;Parent=Msa0152240-mRNA-1
chr1_4 CDS 14812180 14812341 14812180 ID=Msa0152240-mRNA-1:cds;Parent=Msa0152240-mRNA-1
chr1_4 CDS 14812533 14812661 14812533 ID=Msa0152240-mRNA-1:cds;Parent=Msa0152240-mRNA-1
chr1_4 CDS 14812859 14812927 14812859 ID=Msa0152240-mRNA-1:cds;Parent=Msa0152240-mRNA-1
chr1_4 CDS 14813165 14813398 14813165 ID=Msa0152240-mRNA-1:cds;Parent=Msa0152240-mRNA-1
chr1_4 CDS 14813864 14813962 14813864 ID=Msa0152240-mRNA-1:cds;Parent=Msa0152240-mRNA-1
chr1_4 three_prime_UTR 14813963 14814320 14813963 ID=Msa0152240-mRNA-1:three_prime_utr;Parent=Msa0152240-mRNA-1
Gene Sequence

>Msa0152240

ATGGTGTTTTGGAACGTGGAGCAGGTTGTTCTTTTAGTTCTTGATGTTCATGCATGCATTGCAAGAGATCCTCAGCACTTCCGGCATCTTGTCGTTTTCCTTGTGCACAATTTCAGACTAGATAATTCTCTTTTGGAAAAGCGTGGTGCTTTGATAATTCGTCGCCTTTGTGTGCTTTTGAATGCCGAGAGAGTCTACCGTGAACTTTCTACAATATTAGAAGGAGAATCTGATTTGGATTTTGCATCAATTATGGTTCAGGCTTTGAATTTGATCTTACTTACATCATCAGAATTGTCTGAGATTCGAGATCTTTTAAAGCAATCACTGGTAAATCCTGCCGGGAAGGATTTGTATATTTCTTTGTATGCCTCTTGGTGTCATTCTCCAATGGCAATTATAAGTCTCTGCTTTGTAGCTCAGACTTACCGACATGCAAGTGCTGTGATTCAATCTCTGGAGGAGGAGGATATAAATGTGAAGTTTTTGGTCCAACTGGATAAATTGATTCGCTTGCTGGAAACTCCAATCTTTGCTTATCTTAGATTGCAGCTTCTCGATCCTGCAAGATATTTATGGCTATTCAAAGCATTGTATGGTCTTCTGATGTTGCTACCTCAGCAAAGTGCAGCCTTTAAGATACTAAAAACTCGTTTAAAAGCTGTGCCATCGTATTCTTTCAATCGTGCGCAACTGAATAGAGAGCCTTCTGGGGATTATTACCAATTTCTTCCTCAGATGCCTGATGGAACAAAAGAGGATGGTGATGCAACTGAAGATGGTGGGAGCTCATATAATGCAATTAACTTTGCTGCAAGACTACAACAGTTTCAGAAAATGCAGCAGCAACATCGGGAGGCACAACAAGAGGAAGAGCCACAGAAAACTCAGTCTATTGATCTTAATGTCCCTCCTTCAAGATCAAGGAGAAATTCAGGGCAACTACAACTATAA
Protein sequence

>Msa0152240

MVFWNVEQVVLLVLDVHACIARDPQHFRHLVVFLVHNFRLDNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDLDFASIMVQALNLILLTSSELSEIRDLLKQSLVNPAGKDLYISLYASWCHSPMAIISLCFVAQTYRHASAVIQSLEEEDINVKFLVQLDKLIRLLETPIFAYLRLQLLDPARYLWLFKALYGLLMLLPQQSAAFKILKTRLKAVPSYSFNRAQLNREPSGDYYQFLPQMPDGTKEDGDATEDGGSSYNAINFAARLQQFQKMQQQHREAQQEEEPQKTQSIDLNVPPSRSRRNSGQLQL*