AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0182710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0182710 MtrunA17_Chr1g0209921 84.328 268 16 3 1 242 1 268 1.61e-162 453
Msa0182710 MtrunA17_Chr2g0314791 54.571 350 101 8 13 308 1 346 1.73e-124 360
Msa0182710 MtrunA17_Chr2g0314801 40.503 358 143 11 15 307 1 353 1.69e-80 248
Msa0182710 MtrunA17_Chr3g0097891 41.787 347 137 13 22 308 10 351 4.68e-78 242
Msa0182710 MtrunA17_Chr4g0045941 39.601 351 145 13 17 308 9 351 5.19e-71 224
Msa0182710 MtrunA17_Chr2g0283581 41.463 328 127 12 39 307 37 358 8.91e-71 223
Msa0182710 MtrunA17_Chr5g0413851 39.157 332 136 12 39 310 37 362 6.73e-68 216
Msa0182710 MtrunA17_Chr4g0045951 39.583 336 139 12 32 308 33 363 1.91e-67 215
Msa0182710 MtrunA17_Chr3g0085071 38.438 333 139 11 35 307 37 363 1.02e-65 210
Msa0182710 MtrunA17_Chr3g0085081 38.462 338 140 12 31 307 18 348 3.41e-64 206
Msa0182710 MtrunA17_Chr2g0325481 38.906 329 134 13 41 309 47 368 8.03e-64 206
Msa0182710 MtrunA17_Chr3g0091031 37.982 337 143 11 31 307 18 348 9.68e-64 205
Msa0182710 MtrunA17_Chr4g0041011 39.024 328 131 12 41 307 44 363 2.26e-63 204
Msa0182710 MtrunA17_Chr3g0105301 34.682 346 146 13 39 308 36 377 7.81e-57 188
Msa0182710 MtrunA17_Chr5g0430371 34.916 358 146 13 34 308 47 400 1.31e-55 185
Msa0182710 MtrunA17_Chr1g0209931 91.011 89 8 0 228 316 13 101 1.51e-55 176
Msa0182710 MtrunA17_Chr1g0175671 33.043 345 149 12 43 308 169 510 1.01e-51 178
Msa0182710 MtrunA17_Chr4g0068111 33.043 345 149 11 43 308 113 454 1.31e-51 176
Msa0182710 MtrunA17_Chr1g0171171 33.434 332 150 12 41 307 50 375 2.26e-51 174
Msa0182710 MtrunA17_Chr7g0258851 32.951 349 152 12 43 312 247 592 4.18e-51 178
Msa0182710 MtrunA17_Chr1g0210771 33.735 332 148 14 42 307 57 382 2.27e-50 171
Msa0182710 MtrunA17_Chr4g0071391 31.988 347 156 13 42 311 107 450 1.72e-48 168
Msa0182710 MtrunA17_Chr7g0269991 33.434 332 146 14 44 304 137 464 4.13e-48 167
Msa0182710 MtrunA17_Chr4g0006991 31.214 346 156 11 42 307 84 427 1.49e-47 165
Msa0182710 MtrunA17_Chr5g0413271 31.672 341 153 12 42 308 143 477 1.75e-47 166
Msa0182710 MtrunA17_Chr1g0213281 33.333 345 147 13 44 308 175 516 2.03e-46 164
Msa0182710 MtrunA17_Chr8g0368461 30.447 358 152 12 43 308 177 529 1.06e-44 159
Msa0182710 MtrunA17_Chr4g0005481 30.347 346 162 10 39 307 103 446 5.14e-42 150
Msa0182710 MtrunA17_Chr3g0126021 30.792 341 161 13 39 308 124 460 2.64e-41 149
Msa0182710 MtrunA17_Chr8g0360521 28.406 345 166 11 44 310 71 412 4.20e-41 147
Msa0182710 MtrunA17_Chr7g0242701 30.548 347 159 13 41 307 83 427 4.40e-41 147
Msa0182710 MtrunA17_Chr3g0113671 30.857 350 158 12 42 309 104 451 7.86e-41 147
Msa0182710 MtrunA17_Chr8g0384151 31.657 338 152 14 42 308 88 417 1.67e-40 146
Msa0182710 MtrunA17_Chr7g0259711 31.073 354 160 15 41 313 88 438 5.69e-40 145
Msa0182710 MtrunA17_Chr2g0287901 32.948 346 147 14 44 308 97 438 3.87e-39 143
Msa0182710 MtrunA17_Chr4g0005491 27.348 362 179 11 30 308 48 408 5.25e-39 142
Msa0182710 MtrunA17_Chr3g0114101 29.885 348 156 12 44 308 89 431 2.33e-37 138
Msa0182710 MtrunA17_Chr7g0243301 27.684 354 172 12 39 309 59 411 4.04e-35 131
Msa0182710 MtrunA17_Chr5g0437601 27.954 347 168 11 42 308 81 425 5.94e-35 131
Msa0182710 MtrunA17_Chr5g0437291 29.462 353 162 13 42 309 163 513 6.55e-35 132
Msa0182710 MtrunA17_Chr1g0178081 29.651 344 164 16 17 312 33 346 3.40e-32 122
Msa0182710 MtrunA17_Chr1g0188191 26.741 359 157 19 44 307 234 581 1.31e-17 83.2
Msa0182710 MtrunA17_Chr5g0414061 25.496 353 174 17 40 307 90 438 5.18e-16 78.2
Msa0182710 MtrunA17_Chr6g0456681 22.599 354 185 11 39 307 59 408 8.67e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0182710 AT1G78710.1 44.242 330 121 8 38 308 32 357 4.79e-90 273
Msa0182710 AT2G30900.1 40.673 327 129 10 44 308 43 366 2.98e-74 233
Msa0182710 AT1G78710.2 42.000 300 118 8 38 308 25 297 3.43e-73 228
Msa0182710 AT2G34070.1 39.385 325 130 12 44 307 65 383 1.80e-65 211
Msa0182710 AT1G29050.1 38.720 328 135 13 40 307 57 378 2.67e-65 210
Msa0182710 AT2G42570.1 39.577 331 136 13 39 310 42 367 5.09e-64 206
Msa0182710 AT3G14850.2 38.369 331 135 12 41 308 31 355 9.78e-63 203
Msa0182710 AT2G31110.2 37.805 328 140 13 39 307 40 362 7.22e-61 198
Msa0182710 AT5G58600.1 35.294 340 141 11 44 308 66 401 3.26e-55 184
Msa0182710 AT3G54260.1 37.048 332 139 13 44 310 51 377 1.97e-54 182
Msa0182710 AT2G30010.1 35.493 355 142 15 36 308 48 397 4.14e-54 182
Msa0182710 AT2G30010.2 35.493 355 142 15 36 308 91 440 7.10e-54 182
Msa0182710 AT2G30900.2 45.370 216 86 7 44 230 43 255 1.94e-53 176
Msa0182710 AT3G62390.1 35.000 340 145 13 42 308 136 472 3.51e-52 179
Msa0182710 AT5G49340.1 32.759 348 146 12 44 308 100 442 4.35e-52 178
Msa0182710 AT2G34070.2 39.683 252 112 8 92 307 2 249 1.06e-50 169
Msa0182710 AT3G12060.1 32.370 346 152 12 42 308 194 536 6.48e-48 169
Msa0182710 AT3G14850.1 39.044 251 111 8 92 304 2 248 2.83e-47 160
Msa0182710 AT5G20590.1 33.138 341 151 15 44 308 141 480 3.55e-47 166
Msa0182710 AT5G01360.2 30.548 347 158 11 42 307 34 378 5.13e-47 162
Msa0182710 AT5G06700.1 31.214 346 156 14 42 308 253 595 6.92e-47 167
Msa0182710 AT5G01360.1 30.548 347 158 11 42 307 90 434 1.17e-46 163
Msa0182710 AT2G31110.3 41.204 216 95 9 43 230 44 255 2.34e-45 155
Msa0182710 AT1G48880.1 32.641 337 154 13 39 308 110 440 1.48e-44 157
Msa0182710 AT1G60790.1 31.054 351 153 13 42 308 188 533 4.26e-43 155
Msa0182710 AT1G73140.1 29.855 345 161 12 42 308 65 406 3.40e-42 150
Msa0182710 AT5G06230.2 31.285 358 154 14 41 312 18 369 5.74e-41 146
Msa0182710 AT5G06230.1 31.285 358 154 14 41 312 59 410 1.05e-40 147
Msa0182710 AT3G55990.1 29.714 350 162 12 42 309 138 485 3.28e-39 144
Msa0182710 AT5G19160.1 32.203 354 154 17 44 313 99 450 5.21e-39 143
Msa0182710 AT3G06080.2 32.092 349 154 16 44 311 103 449 2.26e-38 142
Msa0182710 AT5G58600.2 38.710 217 99 9 44 230 66 278 6.16e-37 134
Msa0182710 AT3G11570.1 30.982 326 147 13 64 312 100 424 9.74e-36 134
Msa0182710 AT5G01620.2 27.887 355 170 13 34 307 100 449 1.99e-35 133
Msa0182710 AT5G01620.1 27.887 355 170 13 34 307 100 449 1.99e-35 133
Msa0182710 AT2G40320.1 29.107 347 164 13 42 308 79 423 2.30e-35 132
Msa0182710 AT5G01620.3 27.887 355 170 13 34 307 108 457 2.72e-35 133
Msa0182710 AT2G40160.2 29.050 358 151 13 44 308 77 424 1.07e-34 131
Msa0182710 AT2G31110.1 38.462 208 92 8 134 307 9 214 2.43e-34 125
Msa0182710 AT2G40150.1 27.451 357 169 12 43 311 70 424 2.90e-34 130
Msa0182710 AT2G40160.1 28.895 353 148 13 44 303 77 419 4.71e-34 129
Msa0182710 AT2G38320.1 28.693 352 165 14 41 308 56 405 1.28e-33 127
Msa0182710 AT3G62390.2 35.526 228 105 9 42 230 136 360 1.65e-33 127
Msa0182710 AT3G11030.1 28.736 348 165 12 42 308 104 449 3.33e-33 127
Msa0182710 AT5G20590.2 33.778 225 109 10 44 229 141 364 1.35e-31 122
Msa0182710 AT5G19160.2 33.480 227 109 10 44 230 99 323 6.43e-26 105
Msa0182710 AT2G38320.2 27.445 317 147 12 74 308 7 322 3.65e-25 103
Msa0182710 AT3G06080.1 33.921 227 108 11 44 230 103 327 1.45e-24 102
Msa0182710 AT2G40160.3 30.342 234 107 8 44 231 77 300 9.98e-24 99.4
Msa0182710 AT5G06230.3 30.417 240 111 10 41 230 59 292 3.29e-23 97.8
Msa0182710 AT3G28150.1 26.479 355 158 14 43 307 69 410 3.05e-19 87.8

Find 68 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTCTATGTTCAGATTTC+TGG 0.137068 1_4:+71485219 Msa0182710:CDS
CACACACATTCGTTCTTCTA+TGG 0.191029 1_4:+71484552 Msa0182710:CDS
GGTTAACCACTTGGTCTAAT+TGG 0.192861 1_4:+71486363 Msa0182710:CDS
ACATGAAAAGTCATGTTTAT+TGG 0.227466 1_4:+71487070 Msa0182710:CDS
TCAAATCCCTGCACTATAAA+AGG 0.249740 1_4:-71484686 None:intergenic
GTAAAGGTGTAATTTGATTC+TGG 0.257863 1_4:-71485181 None:intergenic
GGATTTGATTGTCTCATAAA+TGG 0.308214 1_4:+71484701 Msa0182710:CDS
CAAACAATCATGGTTTGGTT+TGG 0.329748 1_4:+71484476 None:intergenic
AACATTGAAGCTTACAAGAT+TGG 0.344348 1_4:+71486341 Msa0182710:CDS
CCTCGACCAGTGAATATAGA+AGG 0.368131 1_4:-71487135 None:intergenic
GCATCATAAAGAGGGTAGTA+TGG 0.369410 1_4:-71484650 None:intergenic
AAGATTATCTCAAATATAGA+TGG 0.394741 1_4:+71484735 Msa0182710:CDS
GCTGGCTTTGATCCTTCCTC+TGG 0.398684 1_4:-71487009 None:intergenic
AATGGCAAACAATCATGGTT+TGG 0.409245 1_4:+71484471 None:intergenic
ACATTGAAGCTTACAAGATT+GGG 0.421501 1_4:+71486342 Msa0182710:CDS
AAAGATAACAGTAGTCTTTG+AGG 0.421554 1_4:-71486405 None:intergenic
TGCAGTCACTGGTGTCTAGC+TGG 0.427585 1_4:+71487174 Msa0182710:CDS
AACATTTCATTCCAAGTGTC+GGG 0.442071 1_4:-71487201 None:intergenic
CAAACACAACTGCGGATAGA+TGG 0.442377 1_4:+71487108 Msa0182710:CDS
GTGATCTATCTAAAGGAGAA+TGG 0.447934 1_4:+71484615 Msa0182710:CDS
TCAATAGTGTGGGTGAAAGA+TGG 0.461620 1_4:+71485357 Msa0182710:CDS
TAGCTGGTGCTCCCGACACT+TGG 0.461664 1_4:+71487190 Msa0182710:CDS
TATTTGAGATAATCTTGATC+TGG 0.461942 1_4:-71484728 None:intergenic
TACGGTTCGTGATAGAGAAA+AGG 0.466498 1_4:+71485392 Msa0182710:CDS
CTTGGAAGATCACATGCAAA+AGG 0.466690 1_4:-71484761 None:intergenic
TGATCTATCTAAAGGAGAAT+GGG 0.480088 1_4:+71484616 Msa0182710:CDS
ACACACATTCGTTCTTCTAT+GGG 0.503042 1_4:+71484553 Msa0182710:CDS
CAAGAATGTGATCTATCTAA+AGG 0.504816 1_4:+71484608 Msa0182710:CDS
GGATCAAAGCCAGCATATCC+TGG 0.505064 1_4:+71487018 Msa0182710:CDS
ACAAGATTGGGTTAACCACT+TGG 0.507566 1_4:+71486354 Msa0182710:CDS
CAATAGTGTGGGTGAAAGAT+GGG 0.508616 1_4:+71485358 Msa0182710:CDS
AAGATAACAGTAGTCTTTGA+GGG 0.513061 1_4:-71486404 None:intergenic
AAGACAATATCAAATGACAT+TGG 0.532659 1_4:+71484508 Msa0182710:CDS
ACTCTATAAGCGACAATATG+TGG 0.533074 1_4:+71485125 Msa0182710:intron
CAACATTTCATTCCAAGTGT+CGG 0.533298 1_4:-71487202 None:intergenic
CCACTTGTACCGAGATGAAA+CGG 0.534358 1_4:-71485436 None:intergenic
TCCCTTGAGGCATCATAAAG+AGG 0.534577 1_4:-71484659 None:intergenic
ATGTCTACACCAGGATATGC+TGG 0.536979 1_4:-71487027 None:intergenic
AGTATGAAGTTTCAATAGTG+TGG 0.537445 1_4:+71485346 Msa0182710:CDS
TGGTCGAGGAACTTCTTATG+AGG 0.538598 1_4:+71487149 Msa0182710:CDS
ACGGTTCGTGATAGAGAAAA+GGG 0.539431 1_4:+71485393 Msa0182710:CDS
AACTTGACTCCGTTTCATCT+CGG 0.548645 1_4:+71485427 Msa0182710:CDS
CCCTCTTTATGATGCCTCAA+GGG 0.550562 1_4:+71484658 Msa0182710:CDS
TGTGTGAAACCACCAATGAT+AGG 0.556830 1_4:-71485484 None:intergenic
CTTATGAGGACTGCAGTCAC+TGG 0.559215 1_4:+71487163 Msa0182710:CDS
ATCAATCCAATTAGACCAAG+TGG 0.559355 1_4:-71486369 None:intergenic
AGATGGGTACCTAGTGGATA+CGG 0.563975 1_4:+71485374 Msa0182710:CDS
GGTCACCCTTCTATATTCAC+TGG 0.564907 1_4:+71487129 Msa0182710:CDS
CCGTTTCATCTCGGTACAAG+TGG 0.575787 1_4:+71485436 Msa0182710:CDS
ACCCTCTTTATGATGCCTCA+AGG 0.586390 1_4:+71484657 Msa0182710:CDS
CCTTCTATATTCACTGGTCG+AGG 0.587820 1_4:+71487135 Msa0182710:CDS
TCATCTCGGTACAAGTGGAA+TGG 0.589411 1_4:+71485441 Msa0182710:CDS
GTATGAAGTTTCAATAGTGT+GGG 0.591688 1_4:+71485347 Msa0182710:CDS
ATTCGTTCTTCTATGGGCAG+TGG 0.593313 1_4:+71484559 Msa0182710:CDS
AAATGAGATAATGAAAGACA+TGG 0.606612 1_4:+71486316 Msa0182710:CDS
CCCTTGAGGCATCATAAAGA+GGG 0.607432 1_4:-71484658 None:intergenic
GAACAAACTAAGCCAGAGGA+AGG 0.612792 1_4:+71486997 Msa0182710:intron
CTCGACCAGTGAATATAGAA+GGG 0.620624 1_4:-71487134 None:intergenic
TTCTGAACAATGTCTACACC+AGG 0.627751 1_4:-71487036 None:intergenic
GCATGCACATAGTACAGTGT+AGG 0.634677 1_4:-71484584 None:intergenic
TATCACGAACCGTATCCACT+AGG 0.636942 1_4:-71485383 None:intergenic
CATCCACAGACACTTTCAAG+TGG 0.640996 1_4:+71486292 Msa0182710:intron
TATCATTGGTGGTTTCACAC+AGG 0.651059 1_4:+71485486 Msa0182710:CDS
ATCCACAGACACTTTCAAGT+GGG 0.658544 1_4:+71486293 Msa0182710:intron
TTACTTTACAAACACAACTG+CGG 0.678864 1_4:+71487100 Msa0182710:CDS
TATAAAAGGACAGTCCCTTG+AGG 0.699934 1_4:-71484672 None:intergenic
CATAGAAGAACGAATGTGTG+TGG 0.724411 1_4:-71484551 None:intergenic
GGTGAAAGATGGGTACCTAG+TGG 0.748513 1_4:+71485368 Msa0182710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATAAGATAATAATTTTTC+GGG - chr1_4:71486027-71486046 None:intergenic 10.0%
!!! TATAGAATATTTGATTTTTT+CGG - chr1_4:71486729-71486748 None:intergenic 10.0%
!!! GATGCAATATTTATTTTTAT+TGG + chr1_4:71484901-71484920 Msa0182710:intron 15.0%
!!! CAAACATTTTACATATTATA+AGG - chr1_4:71484934-71484953 None:intergenic 15.0%
!!! TTATTTTAGTCTTAATTTAG+TGG - chr1_4:71484966-71484985 None:intergenic 15.0%
!!! ATTTTCCTTTAATTTAAGAT+TGG - chr1_4:71485260-71485279 None:intergenic 15.0%
!!! AGTTTTTCAATTTTTTACAT+TGG + chr1_4:71486055-71486074 Msa0182710:intron 15.0%
!!! TCTAAAATAATAAATTTGTC+CGG - chr1_4:71486093-71486112 None:intergenic 15.0%
!!! AGTTAGTTTTTTCTTATTAT+TGG + chr1_4:71486158-71486177 Msa0182710:intron 15.0%
!! AAAAAAATGGTATATTTACA+AGG - chr1_4:71486568-71486587 None:intergenic 15.0%
!! TCTTCTCATTTAAAAAAAAA+TGG - chr1_4:71486581-71486600 None:intergenic 15.0%
!!! TGTGAATTTTATAGTTAAAA+GGG - chr1_4:71486930-71486949 None:intergenic 15.0%
!! AAGATTATCTCAAATATAGA+TGG + chr1_4:71484735-71484754 Msa0182710:CDS 20.0%
!! ATGAAACATTATTATTACCT+TGG - chr1_4:71484782-71484801 None:intergenic 20.0%
!!! TATTTAAAATTAGGAAAGAG+TGG - chr1_4:71484817-71484836 None:intergenic 20.0%
!! TCAACTAATACAAAATAACA+AGG - chr1_4:71484883-71484902 None:intergenic 20.0%
!!! TATACAATTAGTTTTGTCTT+AGG - chr1_4:71485018-71485037 None:intergenic 20.0%
!! AACTTATCAAACAATTATCA+AGG - chr1_4:71485289-71485308 None:intergenic 20.0%
!! AATTGTTTGATAAGTTATCT+TGG + chr1_4:71485293-71485312 Msa0182710:intron 20.0%
!! ACTCTATAAATTAATAACGT+CGG + chr1_4:71485666-71485685 Msa0182710:intron 20.0%
!! AAACTCTTTACTTTAATACT+CGG - chr1_4:71486131-71486150 None:intergenic 20.0%
!!! TTTTTCTTATTATTGGCTAT+AGG + chr1_4:71486165-71486184 Msa0182710:intron 20.0%
!!! AATTTTAATCCTAACTTTGA+TGG + chr1_4:71486676-71486695 Msa0182710:intron 20.0%
!!! TTTTAGTCTTTGCAAATATA+CGG - chr1_4:71486818-71486837 None:intergenic 20.0%
!!! GTGTGAATTTTATAGTTAAA+AGG - chr1_4:71486931-71486950 None:intergenic 20.0%
!!! TAATGCTTCATAAATTTTCA+AGG + chr1_4:71486959-71486978 Msa0182710:intron 20.0%
!!! AATGCTTCATAAATTTTCAA+GGG + chr1_4:71486960-71486979 Msa0182710:intron 20.0%
! AAGACAATATCAAATGACAT+TGG + chr1_4:71484508-71484527 Msa0182710:CDS 25.0%
!! TATTTGAGATAATCTTGATC+TGG - chr1_4:71484731-71484750 None:intergenic 25.0%
!!! CTACGTTTTGATTTTGATTT+TGG + chr1_4:71484856-71484875 Msa0182710:intron 25.0%
!!! ATGTTTTGTTAGTCTTGTAA+AGG - chr1_4:71485200-71485219 None:intergenic 25.0%
! CAACTCCAATCTTAAATTAA+AGG + chr1_4:71485252-71485271 Msa0182710:intron 25.0%
!! TGATTTTCAATACCTATCAT+TGG + chr1_4:71485472-71485491 Msa0182710:CDS 25.0%
! GAGAGAGTTATTAGAATATA+AGG + chr1_4:71485623-71485642 Msa0182710:intron 25.0%
! CTCTATAAATTAATAACGTC+GGG + chr1_4:71485667-71485686 Msa0182710:intron 25.0%
! ATTGTACATACCAAACAAAA+TGG + chr1_4:71485774-71485793 Msa0182710:intron 25.0%
!! AGTTTGTTCTTCTTATCATT+CGG - chr1_4:71486201-71486220 None:intergenic 25.0%
! TAAGTATCAAAAGAGAATTG+AGG + chr1_4:71486236-71486255 Msa0182710:intron 25.0%
! AAATGAGATAATGAAAGACA+TGG + chr1_4:71486316-71486335 Msa0182710:CDS 25.0%
!!! GATTTTAATCCATCAAAGTT+AGG - chr1_4:71486688-71486707 None:intergenic 25.0%
!! ATAAGAACAAATTTTGCAAG+AGG + chr1_4:71486758-71486777 Msa0182710:intron 25.0%
! ACATGAAAAGTCATGTTTAT+TGG + chr1_4:71487070-71487089 Msa0182710:CDS 25.0%
CAAGAATGTGATCTATCTAA+AGG + chr1_4:71484608-71484627 Msa0182710:CDS 30.0%
TGATCTATCTAAAGGAGAAT+GGG + chr1_4:71484616-71484635 Msa0182710:CDS 30.0%
GGATTTGATTGTCTCATAAA+TGG + chr1_4:71484701-71484720 Msa0182710:CDS 30.0%
TGTATATTTGCAAGTGATGT+TGG + chr1_4:71485032-71485051 Msa0182710:intron 30.0%
! AGTGATGTTGGTTAATTAAC+AGG + chr1_4:71485044-71485063 Msa0182710:intron 30.0%
TGGTTAATTAACAGGTTTGA+TGG + chr1_4:71485052-71485071 Msa0182710:intron 30.0%
GGAAAAAGATCATGTTTGTT+GGG + chr1_4:71485101-71485120 Msa0182710:intron 30.0%
! GAAAAAGATCATGTTTGTTG+GGG + chr1_4:71485102-71485121 Msa0182710:intron 30.0%
! GTAAAGGTGTAATTTGATTC+TGG - chr1_4:71485184-71485203 None:intergenic 30.0%
TCTTTCTATGTTCAGATTTC+TGG + chr1_4:71485219-71485238 Msa0182710:CDS 30.0%
AGTATGAAGTTTCAATAGTG+TGG + chr1_4:71485346-71485365 Msa0182710:CDS 30.0%
GTATGAAGTTTCAATAGTGT+GGG + chr1_4:71485347-71485366 Msa0182710:CDS 30.0%
! TTTTCAATACCTATCATTGG+TGG + chr1_4:71485475-71485494 Msa0182710:CDS 30.0%
GCTAAAAAACTCTAGTACAT+TGG - chr1_4:71485519-71485538 None:intergenic 30.0%
!!! GACTAATTTGCCATTTTGTT+TGG - chr1_4:71485787-71485806 None:intergenic 30.0%
ACTACTTTGTTCATATGCAT+TGG + chr1_4:71485861-71485880 Msa0182710:intron 30.0%
GTAGAGTAAATGTATACAAC+AGG + chr1_4:71485905-71485924 Msa0182710:intron 30.0%
! TTTGGGACCGAAAATTATAA+AGG + chr1_4:71485996-71486015 Msa0182710:intron 30.0%
TTGGGACCGAAAATTATAAA+GGG + chr1_4:71485997-71486016 Msa0182710:intron 30.0%
! GGAGATCCCTTTATAATTTT+CGG - chr1_4:71486006-71486025 None:intergenic 30.0%
AACATTGAAGCTTACAAGAT+TGG + chr1_4:71486341-71486360 Msa0182710:CDS 30.0%
ACATTGAAGCTTACAAGATT+GGG + chr1_4:71486342-71486361 Msa0182710:CDS 30.0%
AAGATAACAGTAGTCTTTGA+GGG - chr1_4:71486407-71486426 None:intergenic 30.0%
AAAGATAACAGTAGTCTTTG+AGG - chr1_4:71486408-71486427 None:intergenic 30.0%
AAGACTACTGTTATCTTTCA+AGG + chr1_4:71486410-71486429 Msa0182710:intron 30.0%
TTACTTTACAAACACAACTG+CGG + chr1_4:71487100-71487119 Msa0182710:CDS 30.0%
!!! ATGTTGTATGCTGTTTTACT+AGG + chr1_4:71487219-71487238 Msa0182710:CDS 30.0%
ACACACATTCGTTCTTCTAT+GGG + chr1_4:71484553-71484572 Msa0182710:CDS 35.0%
GTGATCTATCTAAAGGAGAA+TGG + chr1_4:71484615-71484634 Msa0182710:CDS 35.0%
TCAAATCCCTGCACTATAAA+AGG - chr1_4:71484689-71484708 None:intergenic 35.0%
!! GTTTGATGGTGTGAAATTCT+TGG + chr1_4:71485066-71485085 Msa0182710:intron 35.0%
! CAAACATGATCTTTTTCCCT+CGG - chr1_4:71485099-71485118 None:intergenic 35.0%
GGGAAAAAGATCATGTTTGT+TGG + chr1_4:71485100-71485119 Msa0182710:intron 35.0%
ACTCTATAAGCGACAATATG+TGG + chr1_4:71485125-71485144 Msa0182710:intron 35.0%
GTCGCATTATTATGTGTTAC+AGG + chr1_4:71485323-71485342 Msa0182710:intron 35.0%
TAAATTAATAACGTCGGGAC+TGG + chr1_4:71485672-71485691 Msa0182710:intron 35.0%
!!! TTTTTTACATTGGCCCAAGT+CGG + chr1_4:71486065-71486084 Msa0182710:intron 35.0%
CAAAAGAGAATTGAGGTTTG+TGG + chr1_4:71486243-71486262 Msa0182710:intron 35.0%
GAATTGAGGTTTGTGGATAT+TGG + chr1_4:71486250-71486269 Msa0182710:intron 35.0%
ATCAATCCAATTAGACCAAG+TGG - chr1_4:71486372-71486391 None:intergenic 35.0%
! AAGAAACCACTGTTTGCTTT+TGG + chr1_4:71486597-71486616 Msa0182710:intron 35.0%
ATCTAACCAAAAGCAAACAG+TGG - chr1_4:71486606-71486625 None:intergenic 35.0%
!! GTTTTAGTCCATGCAAAAGT+TGG + chr1_4:71486631-71486650 Msa0182710:intron 35.0%
! CAATATGACCAACTTTTGCA+TGG - chr1_4:71486642-71486661 None:intergenic 35.0%
AACATTTCATTCCAAGTGTC+GGG - chr1_4:71487204-71487223 None:intergenic 35.0%
CAACATTTCATTCCAAGTGT+CGG - chr1_4:71487205-71487224 None:intergenic 35.0%
CATAGAAGAACGAATGTGTG+TGG - chr1_4:71484554-71484573 None:intergenic 40.0%
CACACACATTCGTTCTTCTA+TGG + chr1_4:71484552-71484571 Msa0182710:CDS 40.0%
GCATCATAAAGAGGGTAGTA+TGG - chr1_4:71484653-71484672 None:intergenic 40.0%
TATAAAAGGACAGTCCCTTG+AGG - chr1_4:71484675-71484694 None:intergenic 40.0%
! GACTGTCCTTTTATAGTGCA+GGG + chr1_4:71484680-71484699 Msa0182710:intron 40.0%
CTTGGAAGATCACATGCAAA+AGG - chr1_4:71484764-71484783 None:intergenic 40.0%
! CTTTTGCATGTGATCTTCCA+AGG + chr1_4:71484762-71484781 Msa0182710:CDS 40.0%
AATTAGGAAAGAGTGGAGAG+TGG - chr1_4:71484810-71484829 None:intergenic 40.0%
AAATTCTTGGAGACATACCG+AGG + chr1_4:71485079-71485098 Msa0182710:intron 40.0%
AATTCTTGGAGACATACCGA+GGG + chr1_4:71485080-71485099 Msa0182710:intron 40.0%
TCAATAGTGTGGGTGAAAGA+TGG + chr1_4:71485357-71485376 Msa0182710:CDS 40.0%
CAATAGTGTGGGTGAAAGAT+GGG + chr1_4:71485358-71485377 Msa0182710:CDS 40.0%
TACGGTTCGTGATAGAGAAA+AGG + chr1_4:71485392-71485411 Msa0182710:CDS 40.0%
ACGGTTCGTGATAGAGAAAA+GGG + chr1_4:71485393-71485412 Msa0182710:CDS 40.0%
AACTTGACTCCGTTTCATCT+CGG + chr1_4:71485427-71485446 Msa0182710:CDS 40.0%
TGTGTGAAACCACCAATGAT+AGG - chr1_4:71485487-71485506 None:intergenic 40.0%
! TATCATTGGTGGTTTCACAC+AGG + chr1_4:71485486-71485505 Msa0182710:CDS 40.0%
AATTACGTTGCAGCGAATCT+TGG + chr1_4:71485819-71485838 Msa0182710:intron 40.0%
ATTACGTTGCAGCGAATCTT+GGG + chr1_4:71485820-71485839 Msa0182710:intron 40.0%
! TTTTTACATTGGCCCAAGTC+GGG + chr1_4:71486066-71486085 Msa0182710:intron 40.0%
! ATCCACAGACACTTTCAAGT+GGG + chr1_4:71486293-71486312 Msa0182710:intron 40.0%
ACAAGATTGGGTTAACCACT+TGG + chr1_4:71486354-71486373 Msa0182710:CDS 40.0%
GGTTAACCACTTGGTCTAAT+TGG + chr1_4:71486363-71486382 Msa0182710:CDS 40.0%
TTCAAGGGAAAAACACTGTC+TGG + chr1_4:71486975-71486994 Msa0182710:intron 40.0%
TTCTGAACAATGTCTACACC+AGG - chr1_4:71487039-71487058 None:intergenic 40.0%
CTCGACCAGTGAATATAGAA+GGG - chr1_4:71487137-71487156 None:intergenic 40.0%
ATTCGTTCTTCTATGGGCAG+TGG + chr1_4:71484559-71484578 Msa0182710:CDS 45.0%
GCATGCACATAGTACAGTGT+AGG - chr1_4:71484587-71484606 None:intergenic 45.0%
ACCCTCTTTATGATGCCTCA+AGG + chr1_4:71484657-71484676 Msa0182710:CDS 45.0%
! CCCTTGAGGCATCATAAAGA+GGG - chr1_4:71484661-71484680 None:intergenic 45.0%
CCCTCTTTATGATGCCTCAA+GGG + chr1_4:71484658-71484677 Msa0182710:CDS 45.0%
! TCCCTTGAGGCATCATAAAG+AGG - chr1_4:71484662-71484681 None:intergenic 45.0%
! GGACTGTCCTTTTATAGTGC+AGG + chr1_4:71484679-71484698 Msa0182710:intron 45.0%
! AGATGGGTACCTAGTGGATA+CGG + chr1_4:71485374-71485393 Msa0182710:CDS 45.0%
TATCACGAACCGTATCCACT+AGG - chr1_4:71485386-71485405 None:intergenic 45.0%
CCACTTGTACCGAGATGAAA+CGG - chr1_4:71485439-71485458 None:intergenic 45.0%
TCATCTCGGTACAAGTGGAA+TGG + chr1_4:71485441-71485460 Msa0182710:CDS 45.0%
CGCTGCAACGTAATTCTAAG+AGG - chr1_4:71485814-71485833 None:intergenic 45.0%
CATCCACAGACACTTTCAAG+TGG + chr1_4:71486292-71486311 Msa0182710:intron 45.0%
!! TTCCCACTTGAAAGTGTCTG+TGG - chr1_4:71486298-71486317 None:intergenic 45.0%
GAATTGCTGCTGCACATTCA+GGG + chr1_4:71486432-71486451 Msa0182710:intron 45.0%
! TCTGGAACAAACTAAGCCAG+AGG + chr1_4:71486993-71487012 Msa0182710:intron 45.0%
GAACAAACTAAGCCAGAGGA+AGG + chr1_4:71486997-71487016 Msa0182710:intron 45.0%
ATGTCTACACCAGGATATGC+TGG - chr1_4:71487030-71487049 None:intergenic 45.0%
CAAACACAACTGCGGATAGA+TGG + chr1_4:71487108-71487127 Msa0182710:CDS 45.0%
GGTCACCCTTCTATATTCAC+TGG + chr1_4:71487129-71487148 Msa0182710:CDS 45.0%
CCTCGACCAGTGAATATAGA+AGG - chr1_4:71487138-71487157 None:intergenic 45.0%
CCTTCTATATTCACTGGTCG+AGG + chr1_4:71487135-71487154 Msa0182710:CDS 45.0%
TGGTCGAGGAACTTCTTATG+AGG + chr1_4:71487149-71487168 Msa0182710:CDS 45.0%
!!! TAAAAATCATATTTAAAATT+AGG - chr1_4:71484826-71484845 None:intergenic 5.0%
!!! TTATTAATTTATGATTTTTT+TGG + chr1_4:71485978-71485997 Msa0182710:intron 5.0%
!!! TATTAATTTATGATTTTTTT+GGG + chr1_4:71485979-71485998 Msa0182710:intron 5.0%
!!! ATAATAAGATAATAATTTTT+CGG - chr1_4:71486028-71486047 None:intergenic 5.0%
!! ATAAAAAATGATATATTTAT+GGG - chr1_4:71486491-71486510 None:intergenic 5.0%
!! AATAAAAAATGATATATTTA+TGG - chr1_4:71486492-71486511 None:intergenic 5.0%
GGTGAAAGATGGGTACCTAG+TGG + chr1_4:71485368-71485387 Msa0182710:CDS 50.0%
CCGTTTCATCTCGGTACAAG+TGG + chr1_4:71485436-71485455 Msa0182710:CDS 50.0%
AATTTGTCCGGTCCCGACTT+GGG - chr1_4:71486081-71486100 None:intergenic 50.0%
AAATTTGTCCGGTCCCGACT+TGG - chr1_4:71486082-71486101 None:intergenic 50.0%
GGAATTGCTGCTGCACATTC+AGG + chr1_4:71486431-71486450 Msa0182710:intron 50.0%
GGATCAAAGCCAGCATATCC+TGG + chr1_4:71487018-71487037 Msa0182710:CDS 50.0%
CTTATGAGGACTGCAGTCAC+TGG + chr1_4:71487163-71487182 Msa0182710:CDS 50.0%
!! GCTGGCTTTGATCCTTCCTC+TGG - chr1_4:71487012-71487031 None:intergenic 55.0%
TGCAGTCACTGGTGTCTAGC+TGG + chr1_4:71487174-71487193 Msa0182710:CDS 55.0%
ACATTGGCCCAAGTCGGGAC+CGG + chr1_4:71486071-71486090 Msa0182710:intron 60.0%
TAGCTGGTGCTCCCGACACT+TGG + chr1_4:71487190-71487209 Msa0182710:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 71484485 71487266 71484485 ID=Msa0182710;Name=Msa0182710
chr1_4 mRNA 71484485 71487266 71484485 ID=Msa0182710-mRNA-1;Parent=Msa0182710;Name=Msa0182710-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0|1|0.4|0.16|6|0|316
chr1_4 exon 71484485 71484694 71484485 ID=Msa0182710-mRNA-1:exon:23909;Parent=Msa0182710-mRNA-1
chr1_4 exon 71484701 71484783 71484701 ID=Msa0182710-mRNA-1:exon:23910;Parent=Msa0182710-mRNA-1
chr1_4 exon 71485135 71485240 71485135 ID=Msa0182710-mRNA-1:exon:23911;Parent=Msa0182710-mRNA-1
chr1_4 exon 71485345 71485520 71485345 ID=Msa0182710-mRNA-1:exon:23912;Parent=Msa0182710-mRNA-1
chr1_4 exon 71486301 71486418 71486301 ID=Msa0182710-mRNA-1:exon:23913;Parent=Msa0182710-mRNA-1
chr1_4 exon 71487009 71487266 71487009 ID=Msa0182710-mRNA-1:exon:23914;Parent=Msa0182710-mRNA-1
chr1_4 CDS 71484485 71484694 71484485 ID=Msa0182710-mRNA-1:cds;Parent=Msa0182710-mRNA-1
chr1_4 CDS 71484701 71484783 71484701 ID=Msa0182710-mRNA-1:cds;Parent=Msa0182710-mRNA-1
chr1_4 CDS 71485135 71485240 71485135 ID=Msa0182710-mRNA-1:cds;Parent=Msa0182710-mRNA-1
chr1_4 CDS 71485345 71485520 71485345 ID=Msa0182710-mRNA-1:cds;Parent=Msa0182710-mRNA-1
chr1_4 CDS 71486301 71486418 71486301 ID=Msa0182710-mRNA-1:cds;Parent=Msa0182710-mRNA-1
chr1_4 CDS 71487009 71487266 71487009 ID=Msa0182710-mRNA-1:cds;Parent=Msa0182710-mRNA-1
Gene Sequence

>Msa0182710

ATGGTTTGGTTTGGAAAGATTTTAAGACAATATCAAATGACATTGGATTTCTTGTTACATCTTCTGCCACACACATTCGTTCTTCTATGGGCAGTGGCACCTACACTGTACTATGTGCATGCTCAAGAATGTGATCTATCTAAAGGAGAATGGGTTGTTGATGATCCATACTACCCTCTTTATGATGCCTCAAGGGACTGTCCTTTTATAGGATTTGATTGTCTCATAAATGGTAGACCAGATCAAGATTATCTCAAATATAGATGGAAACCTTTTGCATGTGATCTTCCAAGCGACAATATGTGGCAGTCACTTGCTTGTTTGCTTCACATTGCAGTGCCAGAATCAAATTACACCTTTACAAGACTAACAAAACATCTTTCTATGTTCAGATTTCTGGAGTATGAAGTTTCAATAGTGTGGGTGAAAGATGGGTACCTAGTGGATACGGTTCGTGATAGAGAAAAGGGAAGAATTATAAAACTTGACTCCGTTTCATCTCGGTACAAGTGGAATGGAGATGTTTTGATTTTCAATACCTATCATTGGTGGTTTCACACAGGACAGACTCCAATACACTTTCAAGTGGGAAATGAGATAATGAAAGACATGGATAACATTGAAGCTTACAAGATTGGGTTAACCACTTGGTCTAATTGGATTGATTCTAACATTGATCCCTCAAAGACTACTCCAGAGGAAGGATCAAAGCCAGCATATCCTGGTGTAGACATTGTTCAGAATGTTTTGAGTAACATGAAAAGTCATGTTTATTGGCTTGACATTACTTTACAAACACAACTGCGGATAGATGGTCACCCTTCTATATTCACTGGTCGAGGAACTTCTTATGAGGACTGCAGTCACTGGTGTCTAGCTGGTGCTCCCGACACTTGGAATGAAATGTTGTATGCTGTTTTACTAGGAAATCAATATTATCGTCATTTGTAA
Protein sequence

>Msa0182710

MVWFGKILRQYQMTLDFLLHLLPHTFVLLWAVAPTLYYVHAQECDLSKGEWVVDDPYYPLYDASRDCPFIGFDCLINGRPDQDYLKYRWKPFACDLPSDNMWQSLACLLHIAVPESNYTFTRLTKHLSMFRFLEYEVSIVWVKDGYLVDTVRDREKGRIIKLDSVSSRYKWNGDVLIFNTYHWWFHTGQTPIHFQVGNEIMKDMDNIEAYKIGLTTWSNWIDSNIDPSKTTPEEGSKPAYPGVDIVQNVLSNMKSHVYWLDITLQTQLRIDGHPSIFTGRGTSYEDCSHWCLAGAPDTWNEMLYAVLLGNQYYRHL*