AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0206620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0206620 MtrunA17_Chr2g0304931 71.154 52 12 1 21 69 213 264 4.85e-18 78.6
Msa0206620 MtrunA17_Chr3g0094811 62.222 45 14 1 21 62 203 247 2.61e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 91 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCACTCGGTTGAAATT+AGG 0.196260 2_1:-36037807 None:intergenic
ATTTAGTGTGAAGGAAATTC+AGG 0.242173 2_1:+36036412 Msa0206620:three_prime_UTR
ATTTAGTGTGAAGGAAATTC+AGG 0.242173 2_1:+36036502 Msa0206620:three_prime_UTR
GCAGATTGGAAATCCTCTTT+TGG 0.283495 2_1:+36035988 Msa0206620:intron
ATCTACTAATTTAAAATACT+TGG 0.300650 2_1:-36036559 None:intergenic
TAATCTTTGATTTAGCATTC+TGG 0.305925 2_1:+36036813 Msa0206620:intron
ACTAATAGTAAGTATTTATA+GGG 0.315455 2_1:-36034782 None:intergenic
TCATGATAAAACCTTTGATT+AGG 0.322904 2_1:-36036201 None:intergenic
TACTAATAGTAAGTATTTAT+AGG 0.326874 2_1:-36034783 None:intergenic
TACTGGTCAAAATCTACTTT+TGG 0.330411 2_1:-36037327 None:intergenic
AAAAGAAGCATACTTGGTTA+TGG 0.347326 2_1:-36037839 None:intergenic
TACTGATAACTGGAATCTTT+AGG 0.349143 2_1:+36037672 Msa0206620:three_prime_UTR
GGTTGCTCTTCCCTTGGCCT+AGG 0.358738 2_1:+36037563 Msa0206620:three_prime_UTR
TAGTCACGTTCCTTTCTTTG+TGG 0.369071 2_1:-36036258 None:intergenic
GGGCTTGGTTGCTCTTCCCT+TGG 0.375330 2_1:+36037557 Msa0206620:intron
ACATTGAAGCTAAGCTAGAT+AGG 0.384366 2_1:-36037779 None:intergenic
GGAGCACCAAGGAGCATTGC+TGG 0.386767 2_1:+36037584 Msa0206620:three_prime_UTR
TGCTAGAATGTACTGATAAC+TGG 0.395632 2_1:+36037662 Msa0206620:three_prime_UTR
CACATCCTGCTGCAAATCCT+TGG 0.406831 2_1:-36036469 None:intergenic
GCTCTTCACAACAACATTGT+AGG 0.407587 2_1:+36036903 Msa0206620:three_prime_UTR
CTTTAGGTTCATAAATATAG+TGG 0.414606 2_1:+36037688 Msa0206620:three_prime_UTR
GGCAGATAGGCTGCATCATC+CGG 0.425976 2_1:+36036716 Msa0206620:three_prime_UTR
ATCAAACGGCAGGAACGAAT+TGG 0.427662 2_1:+36036106 Msa0206620:CDS
GGTTCGTGATCGTTCCTGAC+AGG 0.432018 2_1:+36036158 Msa0206620:CDS
AGTTTGAGCTTCTAATTTCA+AGG 0.434315 2_1:-36036605 None:intergenic
CGAATTTCACGTTCCTGACA+TGG 0.436605 2_1:+36036137 Msa0206620:CDS
ATCTGGTATCTGTGGTCTCA+TGG 0.442871 2_1:+36036028 Msa0206620:CDS
AGATAGAGGGTGGGAGAGAG+TGG 0.446970 2_1:-36034916 None:intergenic
TTGTAAATCTGGAGTAGGTA+TGG 0.464087 2_1:-36036063 None:intergenic
TAGATTTCCTACAGGCAGAT+AGG 0.468461 2_1:+36036703 Msa0206620:three_prime_UTR
TCTTGATCATGAACCCTGTC+AGG 0.471404 2_1:-36036172 None:intergenic
GGAATACATGCTGGAGATAG+AGG 0.482128 2_1:-36034930 None:intergenic
CAATGCTCCTTGGTGCTCCT+AGG 0.482406 2_1:-36037580 None:intergenic
CAGTAATATAATTTGCATAC+TGG 0.483314 2_1:-36037344 None:intergenic
ATAGTAAACGTACGGACTAC+AGG 0.486293 2_1:-36036943 None:intergenic
GAACGATCACGAACCATGTC+AGG 0.488273 2_1:-36036150 None:intergenic
TTAGCATTCTGGTAAGAAGA+TGG 0.489928 2_1:+36036824 Msa0206620:intron
TCCTTCATGAACAGACTAGC+AGG 0.490781 2_1:+36036643 Msa0206620:three_prime_UTR
TGTTGTTGTGAAGAGCTTTG+TGG 0.493225 2_1:-36036896 None:intergenic
TTTAGTGTGAAGGAAATTCA+GGG 0.497321 2_1:+36036413 Msa0206620:three_prime_UTR
TTTAGTGTGAAGGAAATTCA+GGG 0.497321 2_1:+36036503 Msa0206620:three_prime_UTR
TCTTCCACTCTCTTACAACA+TGG 0.499365 2_1:+36034852 Msa0206620:five_prime_UTR
CATGTATTCCTCACTCCTGC+AGG 0.503599 2_1:+36034943 Msa0206620:exon
ACATCCTGCTGCAAATCCTT+GGG 0.505522 2_1:-36036468 None:intergenic
TCTACTAATTTAAAATACTT+GGG 0.506293 2_1:-36036558 None:intergenic
ATGACAGAGTCCACAAAGAA+AGG 0.511144 2_1:+36036248 Msa0206620:CDS
CTAAGATTATTTGTAAATGT+CGG 0.511902 2_1:+36037718 Msa0206620:three_prime_UTR
TCCTTGGTGCTCCTAGGCCA+AGG 0.512421 2_1:-36037574 None:intergenic
CTTCCACTCTCTTACAACAT+GGG 0.521974 2_1:+36034853 Msa0206620:five_prime_UTR
CAAGATTTCGACCTAATCAA+AGG 0.526303 2_1:+36036190 Msa0206620:CDS
ATAATCTTCAGAGACTAGTA+AGG 0.528473 2_1:+36037287 Msa0206620:intron
GGTTCCCAAGGATTTGCAGC+AGG 0.533479 2_1:+36036464 Msa0206620:three_prime_UTR
TGGATGCACTGTTGACGGTA+AGG 0.533820 2_1:+36036443 Msa0206620:three_prime_UTR
GTTCGTGATCGTTCCTGACA+GGG 0.536498 2_1:+36036159 Msa0206620:CDS
TCATAACCAGCAATGCTCCT+TGG 0.537355 2_1:-36037590 None:intergenic
CGATCCAGCTTCAAAACCAT+TGG 0.537499 2_1:+36037419 Msa0206620:three_prime_UTR
CTCTTCACAACAACATTGTA+GGG 0.544259 2_1:+36036904 Msa0206620:three_prime_UTR
AGGAAATTCAGGGTGTGTGT+TGG 0.546733 2_1:+36036423 Msa0206620:three_prime_UTR
AGGAAATTCAGGGTGTGTGT+TGG 0.546733 2_1:+36036513 Msa0206620:three_prime_UTR
GCAATTTCTACTCAATCAAA+CGG 0.549104 2_1:+36036092 Msa0206620:CDS
CAGGAGTGAGGAATACATGC+TGG 0.550463 2_1:-36034939 None:intergenic
ACTGTGTCAAAATCCAAAAG+AGG 0.551073 2_1:-36036001 None:intergenic
CCATTCAACAAAAGCACCAA+TGG 0.551134 2_1:-36037435 None:intergenic
TAGGGAAACGTGACGTGAGT+AGG 0.553841 2_1:-36034764 None:intergenic
AGACCCATGTTGTAAGAGAG+TGG 0.557721 2_1:-36034856 None:intergenic
TGTGTTGGATGCACTGTTGA+CGG 0.570311 2_1:+36036528 Msa0206620:three_prime_UTR
ACCTGCTAGTCTGTTCATGA+AGG 0.572937 2_1:-36036644 None:intergenic
TGTGTTGGATGCACTGTTGA+CGG 0.575637 2_1:+36036438 Msa0206620:three_prime_UTR
CCTTGGTGCTCCTAGGCCAA+GGG 0.575704 2_1:-36037573 None:intergenic
GTTTGAGCTTCTAATTTCAA+GGG 0.586541 2_1:-36036604 None:intergenic
TTTCTACTCAATCAAACGGC+AGG 0.588712 2_1:+36036096 Msa0206620:CDS
CAAACAATCTTGCAAAACAA+AGG 0.594831 2_1:+36036224 Msa0206620:CDS
GTATGTTTAATTTAGTGTGA+AGG 0.597233 2_1:+36036403 Msa0206620:three_prime_UTR
GTATGTTTAATTTAGTGTGA+AGG 0.597233 2_1:+36036493 Msa0206620:three_prime_UTR
TGGATGCACTGTTGACGGCA+AGG 0.597522 2_1:+36036533 Msa0206620:three_prime_UTR
TGTATATACCTGCAGGAGTG+AGG 0.597606 2_1:-36034951 None:intergenic
ATCAGTACATTCTAGCAGCA+AGG 0.600578 2_1:-36037656 None:intergenic
TGTGTGTCTAGATTTCCTAC+AGG 0.601691 2_1:+36036695 Msa0206620:three_prime_UTR
TGTTGACGGTAAGGTTCCCA+AGG 0.602023 2_1:+36036452 Msa0206620:three_prime_UTR
TACATGCTGGAGATAGAGGG+TGG 0.615441 2_1:-36034926 None:intergenic
CAGTGAGTATCTGGTATCTG+TGG 0.618453 2_1:+36036020 Msa0206620:CDS
GAATACATGCTGGAGATAGA+GGG 0.620476 2_1:-36034929 None:intergenic
GGTTTGTCATGAAAATCACT+CGG 0.627575 2_1:-36037818 None:intergenic
CACATCGGAGGTTACATCGA+AGG 0.628243 2_1:-36036676 None:intergenic
ACATGCTGGAGATAGAGGGT+GGG 0.630526 2_1:-36034925 None:intergenic
GATGCAGCCTATCTGCCTGT+AGG 0.632609 2_1:-36036710 None:intergenic
ACATTGTAGGGATCTCACAT+TGG 0.648682 2_1:+36036916 Msa0206620:three_prime_UTR
GTATGCAAATTATATTACTG+TGG 0.665751 2_1:+36037347 Msa0206620:three_prime_UTR
TAGGAAATCTAGACACACAT+CGG 0.677280 2_1:-36036691 None:intergenic
CCCTTGGCCTAGGAGCACCA+AGG 0.693522 2_1:+36037573 Msa0206620:three_prime_UTR
GAAATCTAGACACACATCGG+AGG 0.703734 2_1:-36036688 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+AGG + chr2_1:36035539-36035558 Msa0206620:intron 0.0%
!! TATATATATATATATATATA+GGG + chr2_1:36035540-36035559 Msa0206620:intron 0.0%
!! AATAGATAATAATTGATATT+TGG + chr2_1:36035720-36035739 Msa0206620:intron 10.0%
!! TCAATTATTTATGTTATAAA+AGG + chr2_1:36036966-36036985 Msa0206620:intron 10.0%
!! CAATTATTTATGTTATAAAA+GGG + chr2_1:36036967-36036986 Msa0206620:intron 10.0%
!! AATTATTTATGTTATAAAAG+GGG + chr2_1:36036968-36036987 Msa0206620:intron 10.0%
!! ACTAATAGTAAGTATTTATA+GGG - chr2_1:36034785-36034804 None:intergenic 15.0%
!! TACTAATAGTAAGTATTTAT+AGG - chr2_1:36034786-36034805 None:intergenic 15.0%
!! AGTTTAGACTATAATAAATT+TGG - chr2_1:36035690-36035709 None:intergenic 15.0%
!! TTTGGAAAGAAAAAAAAATA+AGG + chr2_1:36035738-36035757 Msa0206620:intron 15.0%
!! TTGGAAAGAAAAAAAAATAA+GGG + chr2_1:36035739-36035758 Msa0206620:intron 15.0%
!! AACAAAAAAGTAAGAAATTA+TGG + chr2_1:36036297-36036316 Msa0206620:exon 15.0%
!!! TCTACTAATTTAAAATACTT+GGG - chr2_1:36036561-36036580 None:intergenic 15.0%
!!! ATCTACTAATTTAAAATACT+TGG - chr2_1:36036562-36036581 None:intergenic 15.0%
!! TATGCAAATGATAATATATT+CGG + chr2_1:36037473-36037492 Msa0206620:intron 15.0%
!! TAAAAAAATCACGAAATTTG+TGG + chr2_1:36035401-36035420 Msa0206620:intron 20.0%
!!! AACTAATGTGAAAAAGTATA+GGG - chr2_1:36035460-36035479 None:intergenic 20.0%
!!! AAACTAATGTGAAAAAGTAT+AGG - chr2_1:36035461-36035480 None:intergenic 20.0%
!! TAGACTATAATAAATTTGGT+GGG - chr2_1:36035686-36035705 None:intergenic 20.0%
!! TTAGACTATAATAAATTTGG+TGG - chr2_1:36035687-36035706 None:intergenic 20.0%
!! ATATATGAACAGAAAAAGAA+AGG - chr2_1:36036775-36036794 None:intergenic 20.0%
!! ATAATTGAATAGTAAACGTA+CGG - chr2_1:36036954-36036973 None:intergenic 20.0%
!!! TTCTAGATTTTTTTTTTCTC+TGG + chr2_1:36037025-36037044 Msa0206620:intron 20.0%
!!! ACATTTTATATCCTTCAAAT+TGG - chr2_1:36037052-36037071 None:intergenic 20.0%
!! ATTAGCATTAAATACAAAAC+TGG - chr2_1:36037159-36037178 None:intergenic 20.0%
!!! AATAGAAAAGTTTTCTATCT+TGG - chr2_1:36037380-36037399 None:intergenic 20.0%
!!! TTTTTTTTTTGGGATTTTGA+AGG + chr2_1:36037536-36037555 Msa0206620:intron 20.0%
!!! TTTTTTTTTGGGATTTTGAA+GGG + chr2_1:36037537-36037556 Msa0206620:intron 20.0%
!! CTAAGATTATTTGTAAATGT+CGG + chr2_1:36037718-36037737 Msa0206620:three_prime_UTR 20.0%
! TCTCTCAATGAACTAATTAA+CGG + chr2_1:36034986-36035005 Msa0206620:intron 25.0%
!! ACACTTGACAAATTTAAGAT+CGG - chr2_1:36035036-36035055 None:intergenic 25.0%
! ATCTTAAATTTGTCAAGTGT+TGG + chr2_1:36035036-36035055 Msa0206620:intron 25.0%
! TGATAATAATCCCTAACAAA+GGG + chr2_1:36035252-36035271 Msa0206620:intron 25.0%
!!! TCGTGATTTTTTTACAAAGA+GGG - chr2_1:36035395-36035414 None:intergenic 25.0%
!!! TTCGTGATTTTTTTACAAAG+AGG - chr2_1:36035396-36035415 None:intergenic 25.0%
!! AAATTTGAAGTGATAATCAC+AGG + chr2_1:36035763-36035782 Msa0206620:intron 25.0%
! TCATGATAAAACCTTTGATT+AGG - chr2_1:36036204-36036223 None:intergenic 25.0%
!! GTATGTTTAATTTAGTGTGA+AGG + chr2_1:36036403-36036422 Msa0206620:three_prime_UTR 25.0%
!! GTATGTTTAATTTAGTGTGA+AGG + chr2_1:36036403-36036422 Msa0206620:three_prime_UTR 25.0%
! GAATGCTAAATCAAAGATTA+AGG - chr2_1:36036813-36036832 None:intergenic 25.0%
!! TAATCTTTGATTTAGCATTC+TGG + chr2_1:36036813-36036832 Msa0206620:intron 25.0%
!! TATTCAAGTATTTTGCAATG+TGG - chr2_1:36036866-36036885 None:intergenic 25.0%
! TATTTATGTTATAAAAGGGG+TGG + chr2_1:36036971-36036990 Msa0206620:intron 25.0%
! AAATTACTGTTCTATTGAGA+TGG - chr2_1:36037095-36037114 None:intergenic 25.0%
! CTATAACAGAAGAGAAATAT+TGG - chr2_1:36037183-36037202 None:intergenic 25.0%
! AATGAGTGTAAGTAACTATT+AGG - chr2_1:36037213-36037232 None:intergenic 25.0%
!! CAGTAATATAATTTGCATAC+TGG - chr2_1:36037347-36037366 None:intergenic 25.0%
! GTATGCAAATTATATTACTG+TGG + chr2_1:36037347-36037366 Msa0206620:three_prime_UTR 25.0%
!! CTTTAGGTTCATAAATATAG+TGG + chr2_1:36037688-36037707 Msa0206620:three_prime_UTR 25.0%
! TTGTTAGTCTAGTAATCAAG+TGG + chr2_1:36035107-36035126 Msa0206620:intron 30.0%
GTGATAATAATCCCTAACAA+AGG + chr2_1:36035251-36035270 Msa0206620:intron 30.0%
TTACAAAGAGGGAGAAATTA+AGG - chr2_1:36035384-36035403 None:intergenic 30.0%
!! GAGCATCGTATTATGAATTT+TGG - chr2_1:36035429-36035448 None:intergenic 30.0%
!! GGTAAGGTAGTATTGATTAA+CGG - chr2_1:36035843-36035862 None:intergenic 30.0%
! ATTGCAGCTTTTTGTAAATC+TGG - chr2_1:36036077-36036096 None:intergenic 30.0%
GCAATTTCTACTCAATCAAA+CGG + chr2_1:36036092-36036111 Msa0206620:CDS 30.0%
CAAACAATCTTGCAAAACAA+AGG + chr2_1:36036224-36036243 Msa0206620:CDS 30.0%
ATTTAGTGTGAAGGAAATTC+AGG + chr2_1:36036412-36036431 Msa0206620:three_prime_UTR 30.0%
TTTAGTGTGAAGGAAATTCA+GGG + chr2_1:36036413-36036432 Msa0206620:three_prime_UTR 30.0%
ATTTAGTGTGAAGGAAATTC+AGG + chr2_1:36036412-36036431 Msa0206620:three_prime_UTR 30.0%
TTTAGTGTGAAGGAAATTCA+GGG + chr2_1:36036413-36036432 Msa0206620:three_prime_UTR 30.0%
GTTTGAGCTTCTAATTTCAA+GGG - chr2_1:36036607-36036626 None:intergenic 30.0%
AGTTTGAGCTTCTAATTTCA+AGG - chr2_1:36036608-36036627 None:intergenic 30.0%
AATGCATCAAGTGTATATAC+CGG - chr2_1:36036738-36036757 None:intergenic 30.0%
ATAATCTTCAGAGACTAGTA+AGG + chr2_1:36037287-36037306 Msa0206620:intron 30.0%
! TACTGGTCAAAATCTACTTT+TGG - chr2_1:36037330-36037349 None:intergenic 30.0%
! ACTTTTCTATTATTTCGTCG+AGG + chr2_1:36037389-36037408 Msa0206620:three_prime_UTR 30.0%
TACTGATAACTGGAATCTTT+AGG + chr2_1:36037672-36037691 Msa0206620:three_prime_UTR 30.0%
AAAATCACTCGGTTGAAATT+AGG - chr2_1:36037810-36037829 None:intergenic 30.0%
TTAGTTCATTGAGAGAGAGT+GGG - chr2_1:36034982-36035001 None:intergenic 35.0%
ATTAGTTCATTGAGAGAGAG+TGG - chr2_1:36034983-36035002 None:intergenic 35.0%
!!! TAGTTGCTGTTTTAGTTCAG+AGG + chr2_1:36035063-36035082 Msa0206620:intron 35.0%
GATACACGTATGAAATTGCA+AGG + chr2_1:36035292-36035311 Msa0206620:intron 35.0%
! AGACGTTTTCCTATTTGAGT+GGG - chr2_1:36035609-36035628 None:intergenic 35.0%
! TAGACGTTTTCCTATTTGAG+TGG - chr2_1:36035610-36035629 None:intergenic 35.0%
TTGAAGTGATAATCACAGGT+AGG + chr2_1:36035767-36035786 Msa0206620:intron 35.0%
ATACCCTTCAAGTCAATGTT+CGG - chr2_1:36035871-36035890 None:intergenic 35.0%
ACTGTGTCAAAATCCAAAAG+AGG - chr2_1:36036004-36036023 None:intergenic 35.0%
!! ATTTTGACACAGTGAGTATC+TGG + chr2_1:36036011-36036030 Msa0206620:CDS 35.0%
! TTGTAAATCTGGAGTAGGTA+TGG - chr2_1:36036066-36036085 None:intergenic 35.0%
!! GCTTTTTGTAAATCTGGAGT+AGG - chr2_1:36036071-36036090 None:intergenic 35.0%
CAAGATTTCGACCTAATCAA+AGG + chr2_1:36036190-36036209 Msa0206620:CDS 35.0%
TAGGAAATCTAGACACACAT+CGG - chr2_1:36036694-36036713 None:intergenic 35.0%
GCTAAATCAAAGATTAAGGC+AGG - chr2_1:36036809-36036828 None:intergenic 35.0%
TTAGCATTCTGGTAAGAAGA+TGG + chr2_1:36036824-36036843 Msa0206620:intron 35.0%
CTCTTCACAACAACATTGTA+GGG + chr2_1:36036904-36036923 Msa0206620:three_prime_UTR 35.0%
TGGAGTTTAGAAGAATACTG+TGG + chr2_1:36036991-36037010 Msa0206620:intron 35.0%
! TTTTCTCTGGTCCAATTTGA+AGG + chr2_1:36037038-36037057 Msa0206620:intron 35.0%
ACTGGACAAAACGAACTAAA+CGG - chr2_1:36037141-36037160 None:intergenic 35.0%
!! AAATCTACTTTTGGCTGATC+AGG - chr2_1:36037321-36037340 None:intergenic 35.0%
TATATTCGGATGTAAACCAC+TGG + chr2_1:36037487-36037506 Msa0206620:intron 35.0%
! TGCTAGAATGTACTGATAAC+TGG + chr2_1:36037662-36037681 Msa0206620:three_prime_UTR 35.0%
ACATTGAAGCTAAGCTAGAT+AGG - chr2_1:36037782-36037801 None:intergenic 35.0%
GGTTTGTCATGAAAATCACT+CGG - chr2_1:36037821-36037840 None:intergenic 35.0%
TCTTCCACTCTCTTACAACA+TGG + chr2_1:36034852-36034871 Msa0206620:five_prime_UTR 40.0%
CTTCCACTCTCTTACAACAT+GGG + chr2_1:36034853-36034872 Msa0206620:five_prime_UTR 40.0%
GAATACATGCTGGAGATAGA+GGG - chr2_1:36034932-36034951 None:intergenic 40.0%
CTGAAAGCTACCCTTTGTTA+GGG - chr2_1:36035265-36035284 None:intergenic 40.0%
ACTGAAAGCTACCCTTTGTT+AGG - chr2_1:36035266-36035285 None:intergenic 40.0%
TAACAAAGGGTAGCTTTCAG+TGG + chr2_1:36035265-36035284 Msa0206620:intron 40.0%
TACAACACACCCACTCAAAT+AGG + chr2_1:36035597-36035616 Msa0206620:intron 40.0%
TTCAAGTCAATGTTCGGTTG+GGG - chr2_1:36035865-36035884 None:intergenic 40.0%
CTTCAAGTCAATGTTCGGTT+GGG - chr2_1:36035866-36035885 None:intergenic 40.0%
!! CAACCGAACATTGACTTGAA+GGG + chr2_1:36035865-36035884 Msa0206620:intron 40.0%
!! CATTGACTTGAAGGGTATTG+CGG + chr2_1:36035873-36035892 Msa0206620:intron 40.0%
! GCAGATTGGAAATCCTCTTT+TGG + chr2_1:36035988-36036007 Msa0206620:intron 40.0%
TTTCTACTCAATCAAACGGC+AGG + chr2_1:36036096-36036115 Msa0206620:CDS 40.0%
ATGACAGAGTCCACAAAGAA+AGG + chr2_1:36036248-36036267 Msa0206620:CDS 40.0%
TAGTCACGTTCCTTTCTTTG+TGG - chr2_1:36036261-36036280 None:intergenic 40.0%
TGTGTGTCTAGATTTCCTAC+AGG + chr2_1:36036695-36036714 Msa0206620:three_prime_UTR 40.0%
TAGATTTCCTACAGGCAGAT+AGG + chr2_1:36036703-36036722 Msa0206620:three_prime_UTR 40.0%
TGTTGTTGTGAAGAGCTTTG+TGG - chr2_1:36036899-36036918 None:intergenic 40.0%
GCTCTTCACAACAACATTGT+AGG + chr2_1:36036903-36036922 Msa0206620:three_prime_UTR 40.0%
ACATTGTAGGGATCTCACAT+TGG + chr2_1:36036916-36036935 Msa0206620:three_prime_UTR 40.0%
! ATAGTAAACGTACGGACTAC+AGG - chr2_1:36036946-36036965 None:intergenic 40.0%
!!! CAATGGTTTTGAAGCTGGAT+CGG - chr2_1:36037421-36037440 None:intergenic 40.0%
!! CCATTCAACAAAAGCACCAA+TGG - chr2_1:36037438-36037457 None:intergenic 40.0%
!! CCATTGGTGCTTTTGTTGAA+TGG + chr2_1:36037435-36037454 Msa0206620:three_prime_UTR 40.0%
!! TTGGTGCTTTTGTTGAATGG+AGG + chr2_1:36037438-36037457 Msa0206620:three_prime_UTR 40.0%
! GCTTTTGTTGAATGGAGGTA+AGG + chr2_1:36037443-36037462 Msa0206620:intron 40.0%
!!! TTTTGGGATTTTGAAGGGCT+TGG + chr2_1:36037542-36037561 Msa0206620:intron 40.0%
ATCAGTACATTCTAGCAGCA+AGG - chr2_1:36037659-36037678 None:intergenic 40.0%
AGACCCATGTTGTAAGAGAG+TGG - chr2_1:36034859-36034878 None:intergenic 45.0%
GGAATACATGCTGGAGATAG+AGG - chr2_1:36034933-36034952 None:intergenic 45.0%
TGTATATACCTGCAGGAGTG+AGG - chr2_1:36034954-36034973 None:intergenic 45.0%
TCAAGTCAATGTTCGGTTGG+GGG - chr2_1:36035864-36035883 None:intergenic 45.0%
CCTTCAAGTCAATGTTCGGT+TGG - chr2_1:36035867-36035886 None:intergenic 45.0%
!! CCAACCGAACATTGACTTGA+AGG + chr2_1:36035864-36035883 Msa0206620:intron 45.0%
CAATCTGCAAGTGCAAACAC+AGG - chr2_1:36035976-36035995 None:intergenic 45.0%
CTGTGTTTGCACTTGCAGAT+TGG + chr2_1:36035974-36035993 Msa0206620:intron 45.0%
CAGTGAGTATCTGGTATCTG+TGG + chr2_1:36036020-36036039 Msa0206620:CDS 45.0%
ATCTGGTATCTGTGGTCTCA+TGG + chr2_1:36036028-36036047 Msa0206620:CDS 45.0%
ATCAAACGGCAGGAACGAAT+TGG + chr2_1:36036106-36036125 Msa0206620:CDS 45.0%
CGAATTTCACGTTCCTGACA+TGG + chr2_1:36036137-36036156 Msa0206620:CDS 45.0%
TCTTGATCATGAACCCTGTC+AGG - chr2_1:36036175-36036194 None:intergenic 45.0%
AGGAAATTCAGGGTGTGTGT+TGG + chr2_1:36036423-36036442 Msa0206620:three_prime_UTR 45.0%
TGTGTTGGATGCACTGTTGA+CGG + chr2_1:36036438-36036457 Msa0206620:three_prime_UTR 45.0%
ACATCCTGCTGCAAATCCTT+GGG - chr2_1:36036471-36036490 None:intergenic 45.0%
AGGAAATTCAGGGTGTGTGT+TGG + chr2_1:36036423-36036442 Msa0206620:three_prime_UTR 45.0%
TGTGTTGGATGCACTGTTGA+CGG + chr2_1:36036438-36036457 Msa0206620:three_prime_UTR 45.0%
TCCTTCATGAACAGACTAGC+AGG + chr2_1:36036643-36036662 Msa0206620:three_prime_UTR 45.0%
! ACCTGCTAGTCTGTTCATGA+AGG - chr2_1:36036647-36036666 None:intergenic 45.0%
GAAATCTAGACACACATCGG+AGG - chr2_1:36036691-36036710 None:intergenic 45.0%
CGATCCAGCTTCAAAACCAT+TGG + chr2_1:36037419-36037438 Msa0206620:three_prime_UTR 45.0%
!!! AGCACCAATGGTTTTGAAGC+TGG - chr2_1:36037426-36037445 None:intergenic 45.0%
! AGATCAGATCAAAGCACCAG+TGG - chr2_1:36037506-36037525 None:intergenic 45.0%
TCATAACCAGCAATGCTCCT+TGG - chr2_1:36037593-36037612 None:intergenic 45.0%
!!! TTTATTTTAATTTCAAAATA+AGG + chr2_1:36035137-36035156 Msa0206620:intron 5.0%
!! TATTATTATTATTATTATTC+AGG + chr2_1:36035190-36035209 Msa0206620:intron 5.0%
!! ATATATATATATATATATAG+GGG + chr2_1:36035569-36035588 Msa0206620:intron 5.0%
!!! TTACTTTTTTTTTTTTTTTT+TGG + chr2_1:36037525-36037544 Msa0206620:intron 5.0%
!!! TACTTTTTTTTTTTTTTTTT+GGG + chr2_1:36037526-36037545 Msa0206620:intron 5.0%
TAGGGAAACGTGACGTGAGT+AGG - chr2_1:36034767-36034786 None:intergenic 50.0%
ACATGCTGGAGATAGAGGGT+GGG - chr2_1:36034928-36034947 None:intergenic 50.0%
TACATGCTGGAGATAGAGGG+TGG - chr2_1:36034929-36034948 None:intergenic 50.0%
CAGGAGTGAGGAATACATGC+TGG - chr2_1:36034942-36034961 None:intergenic 50.0%
CATGTATTCCTCACTCCTGC+AGG + chr2_1:36034943-36034962 Msa0206620:exon 50.0%
! GGAGAGGTGTATATACCTGC+AGG - chr2_1:36034961-36034980 None:intergenic 50.0%
TCATTGAGAGAGAGTGGGAG+AGG - chr2_1:36034977-36034996 None:intergenic 50.0%
CAAAGGGTAGCTTTCAGTGG+TGG + chr2_1:36035268-36035287 Msa0206620:intron 50.0%
TCAATGTTCGGTTGGGGGTA+AGG - chr2_1:36035859-36035878 None:intergenic 50.0%
GAACGATCACGAACCATGTC+AGG - chr2_1:36036153-36036172 None:intergenic 50.0%
GTTCGTGATCGTTCCTGACA+GGG + chr2_1:36036159-36036178 Msa0206620:CDS 50.0%
TGGATGCACTGTTGACGGTA+AGG + chr2_1:36036443-36036462 Msa0206620:three_prime_UTR 50.0%
TGTTGACGGTAAGGTTCCCA+AGG + chr2_1:36036452-36036471 Msa0206620:three_prime_UTR 50.0%
CACATCCTGCTGCAAATCCT+TGG - chr2_1:36036472-36036491 None:intergenic 50.0%
CACATCGGAGGTTACATCGA+AGG - chr2_1:36036679-36036698 None:intergenic 50.0%
AGATAGAGGGTGGGAGAGAG+TGG - chr2_1:36034919-36034938 None:intergenic 55.0%
GGTTCGTGATCGTTCCTGAC+AGG + chr2_1:36036158-36036177 Msa0206620:CDS 55.0%
GGTTCCCAAGGATTTGCAGC+AGG + chr2_1:36036464-36036483 Msa0206620:three_prime_UTR 55.0%
TGGATGCACTGTTGACGGCA+AGG + chr2_1:36036533-36036552 Msa0206620:three_prime_UTR 55.0%
GATGCAGCCTATCTGCCTGT+AGG - chr2_1:36036713-36036732 None:intergenic 55.0%
GGCAGATAGGCTGCATCATC+CGG + chr2_1:36036716-36036735 Msa0206620:three_prime_UTR 55.0%
!! CAATGCTCCTTGGTGCTCCT+AGG - chr2_1:36037583-36037602 None:intergenic 55.0%
! GGGCTTGGTTGCTCTTCCCT+TGG + chr2_1:36037557-36037576 Msa0206620:intron 60.0%
GGTTGCTCTTCCCTTGGCCT+AGG + chr2_1:36037563-36037582 Msa0206620:three_prime_UTR 60.0%
!! CCTTGGTGCTCCTAGGCCAA+GGG - chr2_1:36037576-36037595 None:intergenic 60.0%
!! TCCTTGGTGCTCCTAGGCCA+AGG - chr2_1:36037577-36037596 None:intergenic 60.0%
!! GGAGCACCAAGGAGCATTGC+TGG + chr2_1:36037584-36037603 Msa0206620:three_prime_UTR 60.0%
!! CCCTTGGCCTAGGAGCACCA+AGG + chr2_1:36037573-36037592 Msa0206620:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 36034746 36037849 36034746 ID=Msa0206620;Name=Msa0206620
chr2_1 mRNA 36034746 36037849 36034746 ID=Msa0206620-mRNA-1;Parent=Msa0206620;Name=Msa0206620-mRNA-1;_AED=0.02;_eAED=0.02;_QI=198|0.8|0.83|1|0.4|0.5|6|1007|125
chr2_1 exon 36034746 36034964 36034746 ID=Msa0206620-mRNA-1:exon:11850;Parent=Msa0206620-mRNA-1
chr2_1 exon 36035212 36035250 36035212 ID=Msa0206620-mRNA-1:exon:11851;Parent=Msa0206620-mRNA-1
chr2_1 exon 36035992 36036737 36035992 ID=Msa0206620-mRNA-1:exon:11852;Parent=Msa0206620-mRNA-1
chr2_1 exon 36036828 36036952 36036828 ID=Msa0206620-mRNA-1:exon:11853;Parent=Msa0206620-mRNA-1
chr2_1 exon 36037298 36037459 36037298 ID=Msa0206620-mRNA-1:exon:11854;Parent=Msa0206620-mRNA-1
chr2_1 exon 36037558 36037849 36037558 ID=Msa0206620-mRNA-1:exon:11855;Parent=Msa0206620-mRNA-1
chr2_1 five_prime_UTR 36034746 36034943 36034746 ID=Msa0206620-mRNA-1:five_prime_utr;Parent=Msa0206620-mRNA-1
chr2_1 CDS 36034944 36034964 36034944 ID=Msa0206620-mRNA-1:cds;Parent=Msa0206620-mRNA-1
chr2_1 CDS 36035212 36035250 36035212 ID=Msa0206620-mRNA-1:cds;Parent=Msa0206620-mRNA-1
chr2_1 CDS 36035992 36036309 36035992 ID=Msa0206620-mRNA-1:cds;Parent=Msa0206620-mRNA-1
chr2_1 three_prime_UTR 36036310 36036737 36036310 ID=Msa0206620-mRNA-1:three_prime_utr;Parent=Msa0206620-mRNA-1
chr2_1 three_prime_UTR 36036828 36036952 36036828 ID=Msa0206620-mRNA-1:three_prime_utr;Parent=Msa0206620-mRNA-1
chr2_1 three_prime_UTR 36037298 36037459 36037298 ID=Msa0206620-mRNA-1:three_prime_utr;Parent=Msa0206620-mRNA-1
chr2_1 three_prime_UTR 36037558 36037849 36037558 ID=Msa0206620-mRNA-1:three_prime_utr;Parent=Msa0206620-mRNA-1
Gene Sequence

>Msa0206620

ATGTATTCCTCACTCCTGCAGGTTATGTCTTCTCTAATTGTGTTCAAAAGAAGAAAAAAAATTGGAAATCCTCTTTTGGATTTTGACACAGTGAGTATCTGGTATCTGTGGTCTCATGGTCTCATAAATGACCATACCTACTCCAGATTTACAAAAAGCTGCAATTTCTACTCAATCAAACGGCAGGAACGAATTGGTAATTTCTCGAATTTCACGTTCCTGACATGGTTCGTGATCGTTCCTGACAGGGTTCATGATCAAGATTTCGACCTAATCAAAGGTTTTATCATGACAAACAATCTTGCAAAACAAAGGAATGACAGAGTCCACAAAGAAAGGAACGTGACTATCAATCTCAAAGTGAAAACAAAAAAGTAA
Protein sequence

>Msa0206620

MYSSLLQVMSSLIVFKRRKKIGNPLLDFDTVSIWYLWSHGLINDHTYSRFTKSCNFYSIKRQERIGNFSNFTFLTWFVIVPDRVHDQDFDLIKGFIMTNNLAKQRNDRVHKERNVTINLKVKTKK*