AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0278110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCACTTCAACTACTTGTT+GGG 0.337129 2_3:-11019031 Msa0278110:CDS
GAGGAAAATCTTACACTTCT+CGG 0.362193 2_3:+11020058 None:intergenic
AATATATGGTTTCAAAAGGT+TGG 0.371174 2_3:-11018726 Msa0278110:three_prime_UTR
GCAGCACTTCAACTACTTGT+TGG 0.416835 2_3:-11019032 Msa0278110:intron
CTTCAACTTTCAGTTGCAAA+AGG 0.428853 2_3:-11019589 Msa0278110:CDS
TTTGAATATATGGTTTCAAA+AGG 0.435804 2_3:-11018730 Msa0278110:three_prime_UTR
AACTAAAATTGAAGAGAAGA+TGG 0.469706 2_3:-11018979 Msa0278110:CDS
CTTCAGCAAACTACAAAGAA+AGG 0.488253 2_3:+11019883 None:intergenic
ATCTTGTGAAGATTTCTGCT+TGG 0.511495 2_3:-11018792 Msa0278110:three_prime_UTR
TTTGCAACTGAAAGTTGAAG+TGG 0.554271 2_3:+11019592 None:intergenic
AGAAATGATGAGGAAGATCA+AGG 0.559760 2_3:+11018870 None:intergenic
GTGAGGGAAGAGAAATGATG+AGG 0.581781 2_3:+11018860 None:intergenic
TACAAAATAGAAGTGAAGAG+TGG 0.617847 2_3:-11018935 Msa0278110:CDS
ACTACAAAGAAAGGAGACAC+TGG 0.626738 2_3:+11019892 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTAATTTTTTTTTTTAC+AGG - chr2_3:11019202-11019221 Msa0278110:intron 10.0%
!!! AAGTTAATTTTTTTTTTTAC+AGG - chr2_3:11019202-11019221 Msa0278110:intron 10.0%
!! AGAATTACTAAATTACTAAT+TGG + chr2_3:11019539-11019558 None:intergenic 15.0%
!! AGAATTACTAAATTACTAAT+TGG + chr2_3:11019539-11019558 None:intergenic 15.0%
!!! ATAAAAGTGGTTTAAAATCA+AGG - chr2_3:11019323-11019342 Msa0278110:intron 20.0%
!! TTTGAATATATGGTTTCAAA+AGG - chr2_3:11020088-11020107 Msa0278110:five_prime_UTR 20.0%
!!! ATAAAAGTGGTTTAAAATCA+AGG - chr2_3:11019323-11019342 Msa0278110:intron 20.0%
!! TTTGAATATATGGTTTCAAA+AGG - chr2_3:11020088-11020107 Msa0278110:five_prime_UTR 20.0%
!! TTTTCCTCTTGAAATGTTTT+TGG - chr2_3:11018775-11018794 Msa0278110:three_prime_UTR 25.0%
! ATTAACTTATCAGATCTACA+AGG + chr2_3:11019190-11019209 None:intergenic 25.0%
!!! TTAAACCACTTTTATTTGCT+AGG + chr2_3:11019318-11019337 None:intergenic 25.0%
!! TTTCAGTATTATTTTCTTGC+AGG + chr2_3:11019481-11019500 None:intergenic 25.0%
! AACTAAAATTGAAGAGAAGA+TGG - chr2_3:11019839-11019858 Msa0278110:five_prime_UTR 25.0%
!! ATGTGATTTTTAACAAGTGA+GGG + chr2_3:11019977-11019996 None:intergenic 25.0%
! AAGTGAGAATGAAAAATATG+AGG - chr2_3:11020057-11020076 Msa0278110:intron 25.0%
!! GGAAAGTGATTTTGAATATA+TGG - chr2_3:11020078-11020097 Msa0278110:five_prime_UTR 25.0%
! AATATATGGTTTCAAAAGGT+TGG - chr2_3:11020092-11020111 Msa0278110:five_prime_UTR 25.0%
!! TTTTCCTCTTGAAATGTTTT+TGG - chr2_3:11018775-11018794 Msa0278110:three_prime_UTR 25.0%
! ATTAACTTATCAGATCTACA+AGG + chr2_3:11019190-11019209 None:intergenic 25.0%
!!! TTAAACCACTTTTATTTGCT+AGG + chr2_3:11019318-11019337 None:intergenic 25.0%
!! TTTCAGTATTATTTTCTTGC+AGG + chr2_3:11019481-11019500 None:intergenic 25.0%
! AACTAAAATTGAAGAGAAGA+TGG - chr2_3:11019839-11019858 Msa0278110:five_prime_UTR 25.0%
!! ATGTGATTTTTAACAAGTGA+GGG + chr2_3:11019977-11019996 None:intergenic 25.0%
! AAGTGAGAATGAAAAATATG+AGG - chr2_3:11020057-11020076 Msa0278110:intron 25.0%
!! GGAAAGTGATTTTGAATATA+TGG - chr2_3:11020078-11020097 Msa0278110:five_prime_UTR 25.0%
! AATATATGGTTTCAAAAGGT+TGG - chr2_3:11020092-11020111 Msa0278110:five_prime_UTR 25.0%
AGATCCAAAAACATTTCAAG+AGG + chr2_3:11018782-11018801 None:intergenic 30.0%
CAGCAGAAAGTTATACTTAA+CGG - chr2_3:11018846-11018865 Msa0278110:three_prime_UTR 30.0%
AGCAGAAAGTTATACTTAAC+GGG - chr2_3:11018847-11018866 Msa0278110:three_prime_UTR 30.0%
GATCAGAAAAAACAAAGAAG+AGG - chr2_3:11019045-11019064 Msa0278110:intron 30.0%
AGATCAGAAAAACAAAGAAG+AGG - chr2_3:11019120-11019139 Msa0278110:intron 30.0%
GAAAACCTAGCAAATAAAAG+TGG - chr2_3:11019310-11019329 Msa0278110:intron 30.0%
TGATCTAGAAATGTTATTGC+AGG + chr2_3:11019633-11019652 None:intergenic 30.0%
!!! TCTCTTCAATTTTAGTTTGC+AGG + chr2_3:11019836-11019855 None:intergenic 30.0%
! ACTCTTCACTTCTATTTTGT+AGG + chr2_3:11019884-11019903 None:intergenic 30.0%
TACAAAATAGAAGTGAAGAG+TGG - chr2_3:11019883-11019902 Msa0278110:five_prime_UTR 30.0%
! GATGTGATTTTTAACAAGTG+AGG + chr2_3:11019978-11019997 None:intergenic 30.0%
AGATCCAAAAACATTTCAAG+AGG + chr2_3:11018782-11018801 None:intergenic 30.0%
CAGCAGAAAGTTATACTTAA+CGG - chr2_3:11018846-11018865 Msa0278110:three_prime_UTR 30.0%
AGCAGAAAGTTATACTTAAC+GGG - chr2_3:11018847-11018866 Msa0278110:three_prime_UTR 30.0%
GATCAGAAAAAACAAAGAAG+AGG - chr2_3:11019045-11019064 Msa0278110:intron 30.0%
AGATCAGAAAAACAAAGAAG+AGG - chr2_3:11019120-11019139 Msa0278110:intron 30.0%
GAAAACCTAGCAAATAAAAG+TGG - chr2_3:11019310-11019329 Msa0278110:intron 30.0%
TGATCTAGAAATGTTATTGC+AGG + chr2_3:11019633-11019652 None:intergenic 30.0%
!!! TCTCTTCAATTTTAGTTTGC+AGG + chr2_3:11019836-11019855 None:intergenic 30.0%
! ACTCTTCACTTCTATTTTGT+AGG + chr2_3:11019884-11019903 None:intergenic 30.0%
TACAAAATAGAAGTGAAGAG+TGG - chr2_3:11019883-11019902 Msa0278110:five_prime_UTR 30.0%
! GATGTGATTTTTAACAAGTG+AGG + chr2_3:11019978-11019997 None:intergenic 30.0%
GAGGAAAATCTTACACTTCT+CGG + chr2_3:11018763-11018782 None:intergenic 35.0%
CGTTAAGTATAACTTTCTGC+TGG + chr2_3:11018848-11018867 None:intergenic 35.0%
CTTCAGCAAACTACAAAGAA+AGG + chr2_3:11018938-11018957 None:intergenic 35.0%
TTTGCAACTGAAAGTTGAAG+TGG + chr2_3:11019229-11019248 None:intergenic 35.0%
CTTCAACTTTCAGTTGCAAA+AGG - chr2_3:11019229-11019248 Msa0278110:intron 35.0%
GTTATTGCAGGAAAGTTGAA+TGG + chr2_3:11019621-11019640 None:intergenic 35.0%
AGAAATGATGAGGAAGATCA+AGG + chr2_3:11019951-11019970 None:intergenic 35.0%
ATCTTGTGAAGATTTCTGCT+TGG - chr2_3:11020026-11020045 Msa0278110:intron 35.0%
GAGGAAAATCTTACACTTCT+CGG + chr2_3:11018763-11018782 None:intergenic 35.0%
CGTTAAGTATAACTTTCTGC+TGG + chr2_3:11018848-11018867 None:intergenic 35.0%
CTTCAGCAAACTACAAAGAA+AGG + chr2_3:11018938-11018957 None:intergenic 35.0%
TTTGCAACTGAAAGTTGAAG+TGG + chr2_3:11019229-11019248 None:intergenic 35.0%
CTTCAACTTTCAGTTGCAAA+AGG - chr2_3:11019229-11019248 Msa0278110:intron 35.0%
GTTATTGCAGGAAAGTTGAA+TGG + chr2_3:11019621-11019640 None:intergenic 35.0%
AGAAATGATGAGGAAGATCA+AGG + chr2_3:11019951-11019970 None:intergenic 35.0%
ATCTTGTGAAGATTTCTGCT+TGG - chr2_3:11020026-11020045 Msa0278110:intron 35.0%
ACTACAAAGAAAGGAGACAC+TGG + chr2_3:11018929-11018948 None:intergenic 40.0%
TCTTGCAGGAAACGTTGAAT+TGG + chr2_3:11019467-11019486 None:intergenic 40.0%
CAGCACTTCAACTACTTGTT+GGG - chr2_3:11019787-11019806 Msa0278110:five_prime_UTR 40.0%
ACTACAAAGAAAGGAGACAC+TGG + chr2_3:11018929-11018948 None:intergenic 40.0%
TCTTGCAGGAAACGTTGAAT+TGG + chr2_3:11019467-11019486 None:intergenic 40.0%
CAGCACTTCAACTACTTGTT+GGG - chr2_3:11019787-11019806 Msa0278110:five_prime_UTR 40.0%
GCAGCACTTCAACTACTTGT+TGG - chr2_3:11019786-11019805 Msa0278110:five_prime_UTR 45.0%
GTGAGGGAAGAGAAATGATG+AGG + chr2_3:11019961-11019980 None:intergenic 45.0%
GCAGCACTTCAACTACTTGT+TGG - chr2_3:11019786-11019805 Msa0278110:five_prime_UTR 45.0%
GTGAGGGAAGAGAAATGATG+AGG + chr2_3:11019961-11019980 None:intergenic 45.0%
!!! ATATTTTTTCAAAAAAAATT+AGG - chr2_3:11019431-11019450 Msa0278110:intron 5.0%
!! TATAAATATAAATAATGAAA+AGG - chr2_3:11019669-11019688 Msa0278110:intron 5.0%
!!! ATAAAATTTTAAAATTAAGT+TGG + chr2_3:11020008-11020027 None:intergenic 5.0%
!!! ATATTTTTTCAAAAAAAATT+AGG - chr2_3:11019431-11019450 Msa0278110:intron 5.0%
!! TATAAATATAAATAATGAAA+AGG - chr2_3:11019669-11019688 Msa0278110:intron 5.0%
!!! ATAAAATTTTAAAATTAAGT+TGG + chr2_3:11020008-11020027 None:intergenic 5.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 11018725 11020115 11018725 ID=Msa0278110;Name=Msa0278110
chr2_3 mRNA 11018725 11020115 11018725 ID=Msa0278110-mRNA-1;Parent=Msa0278110;Name=Msa0278110-mRNA-1;_AED=0.21;_eAED=0.21;_QI=263|0.66|0.75|1|0.66|0.5|4|162|82
chr2_3 exon 11018725 11019050 11018725 ID=Msa0278110-mRNA-1:exon:4922;Parent=Msa0278110-mRNA-1
chr2_3 exon 11019577 11019616 11019577 ID=Msa0278110-mRNA-1:exon:4921;Parent=Msa0278110-mRNA-1
chr2_3 exon 11019699 11019962 11019699 ID=Msa0278110-mRNA-1:exon:4920;Parent=Msa0278110-mRNA-1
chr2_3 exon 11020072 11020115 11020072 ID=Msa0278110-mRNA-1:exon:4919;Parent=Msa0278110-mRNA-1
chr2_3 five_prime_UTR 11020072 11020115 11020072 ID=Msa0278110-mRNA-1:five_prime_utr;Parent=Msa0278110-mRNA-1
chr2_3 five_prime_UTR 11019744 11019962 11019744 ID=Msa0278110-mRNA-1:five_prime_utr;Parent=Msa0278110-mRNA-1
chr2_3 CDS 11019699 11019743 11019699 ID=Msa0278110-mRNA-1:cds;Parent=Msa0278110-mRNA-1
chr2_3 CDS 11019577 11019616 11019577 ID=Msa0278110-mRNA-1:cds;Parent=Msa0278110-mRNA-1
chr2_3 CDS 11018887 11019050 11018887 ID=Msa0278110-mRNA-1:cds;Parent=Msa0278110-mRNA-1
chr2_3 three_prime_UTR 11018725 11018886 11018725 ID=Msa0278110-mRNA-1:three_prime_utr;Parent=Msa0278110-mRNA-1
Gene Sequence

>Msa0278110

ATGAAGATAAATACAAAAAATGAAGATCAGAAAAACAAAGAAGAGGACCACTTCAACTTTCAGTTGCAAAAGGAACAACTTTCAACACTTCAACTACTTGTTGGGTTGAGTAAAGAACTTTCAAGTTTCCTGCAAACTAAAATTGAAGAGAAGATGGAGATTAGAAAGTGTGAAAACCTACAAAATAGAAGTGAAGAGTGGAAGATGAAGATTAGAAAGTTTGAAAAGAAGAAAAAATATAGACCTTGA

Protein sequence

>Msa0278110

MKINTKNEDQKNKEEDHFNFQLQKEQLSTLQLLVGLSKELSSFLQTKIEEKMEIRKCENLQNRSEEWKMKIRKFEKKKKYRP*