AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0646470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646470 MtrunA17_Chr4g0034871 95.631 206 0 1 1 197 1 206 6.87e-145 401
Msa0646470 MtrunA17_Chr4g0004661 84.058 207 23 2 1 197 57 263 3.83e-127 358
Msa0646470 MtrunA17_Chr2g0331691 81.463 205 28 2 3 197 5 209 2.44e-122 344
Msa0646470 MtrunA17_Chr6g0466301 69.082 207 53 3 1 197 1 206 1.04e-100 289
Msa0646470 MtrunA17_Chr6g0465491 69.082 207 53 3 1 197 1 206 1.04e-100 289
Msa0646470 MtrunA17_Chr1g0201121 67.464 209 54 3 1 197 1 207 2.00e-97 281
Msa0646470 MtrunA17_Chr8g0383261 61.576 203 68 2 4 197 8 209 1.64e-89 261
Msa0646470 MtrunA17_Chr6g0466161 55.307 179 79 1 19 197 10 187 1.12e-66 203
Msa0646470 MtrunA17_Chr6g0465471 55.307 179 79 1 19 197 10 187 2.39e-66 202
Msa0646470 MtrunA17_Chr6g0466431 67.327 101 33 0 1 101 1 101 7.82e-48 152
Msa0646470 MtrunA17_Chr6g0465611 67.327 101 33 0 1 101 1 101 7.82e-48 152
Msa0646470 MtrunA17_Chr4g0032201 37.019 208 104 6 8 195 12 212 4.78e-37 128
Msa0646470 MtrunA17_Chr1g0182321 37.931 203 103 8 2 190 5 198 1.30e-35 124
Msa0646470 MtrunA17_Chr2g0276941 36.323 223 102 8 1 190 1 216 1.54e-35 124
Msa0646470 MtrunA17_Chr1g0182301 37.438 203 104 8 2 190 5 198 4.57e-35 123
Msa0646470 MtrunA17_Chr2g0276931 38.690 168 87 4 8 166 9 169 1.94e-34 120
Msa0646470 MtrunA17_Chr7g0263071 38.342 193 100 7 8 190 15 198 2.72e-34 120
Msa0646470 MtrunA17_Chr1g0212521 38.144 194 95 7 8 192 15 192 4.49e-34 120
Msa0646470 MtrunA17_Chr4g0030911 37.563 197 104 7 8 194 15 202 5.66e-34 120
Msa0646470 MtrunA17_Chr5g0400131 36.364 209 100 7 10 197 11 207 6.31e-34 119
Msa0646470 MtrunA17_Chr2g0283131 35.714 210 105 8 8 195 13 214 1.46e-33 119
Msa0646470 MtrunA17_Chr2g0327911 39.286 168 88 5 8 166 15 177 2.48e-33 118
Msa0646470 MtrunA17_Chr4g0055081 35.577 208 102 7 10 197 11 206 1.26e-32 116
Msa0646470 MtrunA17_Chr7g0250531 35.500 200 109 6 8 195 8 199 2.21e-32 115
Msa0646470 MtrunA17_Chr3g0112681 38.095 168 88 4 8 166 12 172 2.30e-32 116
Msa0646470 MtrunA17_Chr4g0055651 38.095 168 88 4 8 166 8 168 2.57e-32 115
Msa0646470 MtrunA17_Chr8g0335471 35.000 200 111 5 8 195 8 200 3.35e-32 115
Msa0646470 MtrunA17_Chr4g0046261 34.286 210 108 8 8 195 13 214 3.61e-32 115
Msa0646470 MtrunA17_Chr8g0382221 36.585 205 105 7 10 197 11 207 2.07e-31 113
Msa0646470 MtrunA17_Chr3g0123971 35.784 204 107 5 10 197 11 206 5.52e-31 112
Msa0646470 MtrunA17_Chr4g0040781 33.333 210 110 8 8 195 13 214 8.08e-31 112
Msa0646470 MtrunA17_Chr1g0174281 35.829 187 104 4 8 185 15 194 9.72e-30 109
Msa0646470 MtrunA17_Chr6g0458081 36.628 172 93 4 8 170 6 170 1.43e-29 108
Msa0646470 MtrunA17_Chr8g0390561 36.471 170 92 4 10 170 11 173 3.21e-29 107
Msa0646470 MtrunA17_Chr3g0123821 34.184 196 111 5 8 194 16 202 3.97e-29 107
Msa0646470 MtrunA17_Chr8g0351861 38.272 162 84 5 8 160 16 170 1.02e-28 107
Msa0646470 MtrunA17_Chr2g0315551 32.536 209 113 8 8 195 13 214 1.15e-28 106
Msa0646470 MtrunA17_Chr3g0109721 36.364 165 89 4 10 165 12 169 1.98e-28 105
Msa0646470 MtrunA17_Chr7g0214241 33.929 168 95 4 8 166 15 175 3.48e-28 105
Msa0646470 MtrunA17_Chr2g0325091 33.014 209 111 8 8 195 11 211 4.54e-28 105
Msa0646470 MtrunA17_Chr2g0290891 34.171 199 112 5 8 194 6 197 6.40e-28 104
Msa0646470 MtrunA17_Chr5g0433561 35.758 165 90 4 10 165 12 169 8.11e-28 103
Msa0646470 MtrunA17_Chr8g0381841 34.545 165 92 4 8 163 17 174 1.25e-27 103
Msa0646470 MtrunA17_Chr7g0231131 37.576 165 87 4 8 163 15 172 1.38e-27 103
Msa0646470 MtrunA17_Chr1g0189361 33.929 168 95 4 8 166 14 174 4.97e-27 102
Msa0646470 MtrunA17_Chr2g0319951 34.545 165 92 3 8 163 13 170 2.16e-26 100
Msa0646470 MtrunA17_Chr7g0234201 32.738 168 97 4 8 166 13 173 3.36e-26 100
Msa0646470 MtrunA17_Chr8g0346421 33.032 221 117 9 1 197 19 232 3.76e-26 100
Msa0646470 MtrunA17_Chr6g0482721 35.185 162 89 4 8 160 12 166 4.48e-26 99.8
Msa0646470 MtrunA17_Chr8g0349711 32.571 175 102 4 5 170 3 170 1.85e-25 98.2
Msa0646470 MtrunA17_Chr8g0349681 35.673 171 94 4 9 170 176 339 2.06e-25 102
Msa0646470 MtrunA17_Chr8g0349681 33.981 206 113 7 8 197 384 582 8.04e-24 98.2
Msa0646470 MtrunA17_Chr8g0349681 33.136 169 97 4 5 164 10 171 5.51e-22 92.8
Msa0646470 MtrunA17_Chr7g0275991 36.420 162 87 4 8 160 20 174 4.20e-25 97.4
Msa0646470 MtrunA17_Chr6g0465601 57.143 70 30 0 99 168 3 72 4.52e-25 93.2
Msa0646470 MtrunA17_Chr1g0199331 34.132 167 93 5 9 165 32 191 8.74e-25 95.9
Msa0646470 MtrunA17_Chr8g0392931 35.185 162 89 4 8 160 14 168 9.76e-25 96.3
Msa0646470 MtrunA17_Chr6g0466421 59.091 66 27 0 103 168 17 82 4.38e-24 90.9
Msa0646470 MtrunA17_Chr8g0384311 33.140 172 100 5 8 170 13 178 6.06e-24 94.0
Msa0646470 MtrunA17_Chr1g0159481 33.721 172 99 5 8 170 17 182 5.98e-23 91.7
Msa0646470 MtrunA17_Chr7g0265001 34.545 165 92 6 10 164 18 176 1.05e-21 88.2
Msa0646470 MtrunA17_Chr1g0197331 32.990 194 110 6 9 191 16 200 2.07e-21 87.4
Msa0646470 MtrunA17_Chr1g0180291 30.208 192 111 6 9 191 15 192 1.20e-20 85.5
Msa0646470 MtrunA17_Chr3g0130271 31.285 179 107 6 2 170 6 178 1.36e-20 85.1
Msa0646470 MtrunA17_Chr2g0326141 64.912 57 20 0 120 176 299 355 1.28e-17 79.3
Msa0646470 MtrunA17_Chr3g0138121 28.313 166 100 4 9 165 14 169 5.85e-17 75.9
Msa0646470 MtrunA17_Chr3g0138101 29.480 173 89 7 9 165 14 169 5.85e-17 75.9
Msa0646470 MtrunA17_Chr3g0138111 29.480 173 89 7 9 165 14 169 6.49e-17 75.9
Msa0646470 MtrunA17_Chr6g0486041 27.670 206 117 6 8 195 6 197 7.85e-15 70.1
Msa0646470 MtrunA17_Chr2g0325101 26.816 179 107 5 8 168 6 178 2.62e-11 60.1
Msa0646470 MtrunA17_Chr3g0117931 26.425 193 118 5 1 175 1 187 6.28e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646470 AT3G18820.1 88.835 206 14 1 1 197 1 206 1.41e-135 378
Msa0646470 AT1G49300.2 84.951 206 22 1 1 197 1 206 1.99e-129 362
Msa0646470 AT1G49300.1 84.951 206 22 1 1 197 1 206 1.99e-129 362
Msa0646470 AT1G52280.1 79.612 206 33 1 1 197 1 206 1.54e-120 340
Msa0646470 AT3G16100.1 78.155 206 36 1 1 197 1 206 3.82e-120 339
Msa0646470 AT4G09720.1 66.505 206 60 1 1 197 1 206 3.78e-101 291
Msa0646470 AT4G09720.4 64.929 211 60 2 1 197 1 211 8.50e-98 282
Msa0646470 AT4G09720.3 63.134 217 60 2 1 197 1 217 1.63e-97 282
Msa0646470 AT1G22740.1 66.667 207 55 3 1 197 1 203 4.02e-96 278
Msa0646470 AT2G21880.1 60.476 210 65 3 4 197 5 212 1.10e-87 257
Msa0646470 AT4G09720.6 67.836 171 55 0 27 197 2 172 7.81e-87 253
Msa0646470 AT4G09720.5 67.836 171 55 0 27 197 2 172 7.81e-87 253
Msa0646470 AT4G09720.2 67.836 171 55 0 27 197 2 172 7.81e-87 253
Msa0646470 AT2G21880.4 61.828 186 62 2 19 197 4 187 6.77e-81 239
Msa0646470 AT2G21880.3 61.828 186 62 2 19 197 4 187 6.77e-81 239
Msa0646470 AT2G21880.2 56.667 210 65 4 4 197 5 204 4.93e-78 232
Msa0646470 AT5G39620.1 55.233 172 68 1 6 168 3 174 1.01e-64 198
Msa0646470 AT5G59840.1 37.563 197 107 6 8 194 15 205 7.17e-36 125
Msa0646470 AT3G53610.2 38.021 192 103 6 8 189 15 200 4.97e-35 123
Msa0646470 AT3G53610.3 38.021 192 103 6 8 189 15 200 4.97e-35 123
Msa0646470 AT3G53610.1 38.021 192 103 6 8 189 15 200 4.97e-35 123
Msa0646470 AT3G46060.2 36.548 197 109 6 8 194 15 205 1.64e-34 122
Msa0646470 AT3G46060.3 36.548 197 109 6 8 194 15 205 1.64e-34 122
Msa0646470 AT3G46060.1 36.548 197 109 6 8 194 15 205 1.64e-34 122
Msa0646470 AT1G09630.1 38.596 171 89 4 8 169 12 175 3.81e-34 120
Msa0646470 AT1G28550.1 35.533 197 101 6 8 185 13 202 7.07e-34 120
Msa0646470 AT2G33870.1 34.286 210 109 7 8 195 13 215 7.70e-34 120
Msa0646470 AT3G11730.1 38.764 178 93 4 1 169 1 171 4.33e-33 117
Msa0646470 AT2G44610.1 36.190 210 100 7 10 197 11 208 7.28e-33 117
Msa0646470 AT4G18430.1 33.333 210 110 8 8 195 13 214 1.26e-32 117
Msa0646470 AT4G17530.1 35.176 199 111 5 8 195 8 199 1.73e-32 116
Msa0646470 AT5G47200.1 35.176 199 111 5 8 195 8 199 3.40e-32 115
Msa0646470 AT5G60860.1 34.762 210 107 8 8 195 13 214 5.10e-32 115
Msa0646470 AT1G07410.1 34.135 208 109 7 8 195 12 211 5.38e-32 115
Msa0646470 AT5G03520.1 41.341 179 84 8 2 166 5 176 7.63e-32 115
Msa0646470 AT3G15060.1 34.286 210 108 8 8 195 13 214 7.66e-32 115
Msa0646470 AT3G46830.1 38.182 165 86 4 8 163 12 169 9.29e-32 114
Msa0646470 AT3G46830.2 38.182 165 86 4 8 163 12 169 9.29e-32 114
Msa0646470 AT5G45750.1 34.450 209 108 9 8 195 13 213 9.77e-32 114
Msa0646470 AT5G59150.1 36.970 165 88 4 8 163 12 169 1.23e-31 114
Msa0646470 AT3G12160.1 36.842 190 104 4 8 188 15 197 2.27e-31 114
Msa0646470 AT3G09900.1 41.341 179 84 8 2 166 5 176 3.92e-31 113
Msa0646470 AT5G64990.1 36.842 209 100 7 10 197 9 206 8.04e-31 112
Msa0646470 AT1G02130.1 34.000 200 113 6 8 195 8 200 1.96e-30 111
Msa0646470 AT4G35860.1 36.842 171 92 4 8 169 6 169 7.44e-30 109
Msa0646470 AT4G18800.1 34.783 184 94 5 8 173 13 188 1.90e-29 108
Msa0646470 AT1G05810.1 31.776 214 119 6 4 197 8 214 4.34e-29 108
Msa0646470 AT5G47960.1 33.019 212 111 6 8 195 15 219 5.55e-29 107
Msa0646470 AT5G10260.1 34.615 208 105 7 10 197 11 207 1.20e-28 106
Msa0646470 AT1G05810.2 31.776 214 119 6 4 197 54 260 1.31e-28 107
Msa0646470 AT1G16920.1 32.212 208 114 8 8 195 13 213 2.03e-28 106
Msa0646470 AT4G39990.1 32.990 194 109 5 8 192 17 198 2.30e-28 106
Msa0646470 AT5G03520.2 44.697 132 67 4 35 166 41 166 2.84e-28 105
Msa0646470 AT5G65270.1 34.054 185 98 5 8 175 17 194 3.01e-28 105
Msa0646470 AT5G64990.2 35.648 216 100 8 10 197 9 213 4.16e-28 105
Msa0646470 AT2G31680.1 33.333 168 96 4 8 166 12 172 4.50e-28 105
Msa0646470 AT4G17170.1 34.171 199 112 5 8 194 6 197 5.24e-28 105
Msa0646470 AT4G17160.1 36.471 170 92 4 9 169 7 169 5.73e-28 104
Msa0646470 AT1G06400.1 35.152 165 91 4 8 163 13 170 7.07e-28 104
Msa0646470 AT1G73640.1 37.037 162 86 4 8 160 13 167 2.08e-27 103
Msa0646470 AT4G19640.1 33.880 183 102 5 10 180 12 187 4.74e-27 102
Msa0646470 AT5G45130.1 35.152 165 91 4 10 165 12 169 7.58e-27 101
Msa0646470 AT1G18200.1 36.970 165 88 5 8 163 13 170 8.53e-27 102
Msa0646470 AT2G43130.1 33.333 168 96 4 8 166 12 172 1.58e-26 101
Msa0646470 AT4G39890.1 35.152 165 92 3 9 164 10 168 2.11e-26 100
Msa0646470 AT1G01200.1 34.320 169 96 3 1 160 21 183 2.72e-26 101
Msa0646470 AT2G22290.1 32.843 204 112 5 10 197 11 205 6.85e-26 99.4
Msa0646470 AT5G47520.1 33.333 168 96 4 8 166 14 174 9.23e-26 99.4
Msa0646470 AT5G45130.2 34.969 163 90 4 12 165 22 177 1.18e-25 99.0
Msa0646470 AT3G07410.1 32.143 168 98 4 8 166 12 172 2.86e-25 98.2
Msa0646470 AT3G54840.1 32.955 176 100 6 1 165 25 193 3.29e-24 95.1
Msa0646470 AT5G59150.2 39.024 123 68 3 41 163 3 118 7.48e-24 93.2
Msa0646470 AT3G54840.3 33.333 165 93 5 11 165 2 159 1.42e-23 92.4
Msa0646470 AT3G54840.2 32.558 172 98 6 1 161 25 189 4.72e-23 91.7
Msa0646470 AT4G35860.2 40.476 126 67 4 41 165 2 120 5.04e-23 90.9
Msa0646470 AT1G43890.3 34.302 172 98 6 8 170 13 178 6.33e-23 91.7
Msa0646470 AT1G43890.1 34.302 172 98 6 8 170 13 178 6.33e-23 91.7
Msa0646470 AT1G43890.2 34.302 172 98 6 8 170 13 178 6.33e-23 91.7
Msa0646470 AT4G19640.2 34.307 137 76 3 10 137 12 143 1.59e-21 86.7
Msa0646470 AT5G03530.2 32.461 191 106 7 10 191 15 191 3.11e-21 87.4
Msa0646470 AT5G03530.1 32.461 191 106 7 10 191 15 191 3.11e-21 87.4
Msa0646470 AT4G19640.3 35.833 120 64 2 10 120 12 127 2.59e-20 84.3
Msa0646470 AT3G09910.3 30.052 193 110 7 9 191 14 191 1.53e-18 80.1
Msa0646470 AT3G09910.1 30.052 193 110 7 9 191 14 191 1.53e-18 80.1
Msa0646470 AT3G09910.2 30.052 193 110 7 9 191 14 191 1.53e-18 80.1
Msa0646470 AT3G09910.5 31.515 165 98 5 9 164 14 172 5.51e-18 78.6
Msa0646470 AT3G09910.4 31.515 165 98 5 9 164 14 172 5.51e-18 78.6
Msa0646470 AT5G55190.1 29.480 173 89 7 9 165 14 169 7.51e-17 75.9
Msa0646470 AT5G20010.1 29.480 173 89 7 9 165 14 169 9.14e-17 75.9
Msa0646470 AT5G20020.1 29.480 173 89 7 9 165 14 169 9.53e-17 75.9
Msa0646470 AT5G55080.1 26.455 189 116 4 9 184 14 192 1.24e-15 72.8
Msa0646470 AT4G28950.1 29.189 185 109 6 8 176 6 184 2.46e-15 71.6
Msa0646470 AT5G62880.1 27.807 187 111 5 1 169 1 181 5.89e-12 62.8
Msa0646470 AT5G45970.1 26.667 180 108 5 8 169 6 179 8.40e-12 62.0
Msa0646470 AT3G48040.1 27.807 187 111 5 1 169 1 181 5.70e-11 59.7

Find 67 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCAGATGTCTCAAAATA+TGG 0.180049 4_3:+52238577 None:intergenic
ATCTTCAAATTGCACTTCTT+TGG 0.266812 4_3:+52240138 None:intergenic
TTCGACATTAATACCTTCTT+TGG 0.296445 4_3:+52238558 None:intergenic
TCTCTCTATCGTAAATCTTA+AGG 0.319379 4_3:+52241408 None:intergenic
GGGCATCCTTTGCTATGGTT+TGG 0.331267 4_3:+52238527 None:intergenic
AAGAAGTGCAATTTGAAGAT+AGG 0.335741 4_3:-52240135 Msa0646470:CDS
CGATTTGTTGGGTGCTTGTT+TGG 0.339053 4_3:-52237807 Msa0646470:three_prime_UTR
ATTCCTCCCTCCAGTTGTTA+AGG 0.391109 4_3:+52239009 None:intergenic
CATTTGACAACCTTAACAAC+TGG 0.410397 4_3:-52239019 Msa0646470:CDS
AGAAAGCAACTCCTAGGCTT+TGG 0.417205 4_3:+52239093 None:intergenic
AGCAATCAGCCAAGGTCATC+AGG 0.423555 4_3:-52237974 Msa0646470:CDS
CTCTTGAAAGTCATCATTCT+CGG 0.427482 4_3:-52241234 Msa0646470:CDS
CTTTCAAGAGCGTTCTTCTA+CGG 0.436158 4_3:+52241247 None:intergenic
GCAAAGGATGCCCTGAAAAG+TGG 0.450910 4_3:-52238517 Msa0646470:CDS
TTGTTTCCTATAACAACAAA+AGG 0.451159 4_3:+52238815 None:intergenic
ACAACAAAAGGAAAATTCTC+TGG 0.451708 4_3:+52238827 None:intergenic
GGATCAAGAGTTAACCGCTT+TGG 0.456479 4_3:+52241288 None:intergenic
TTTCCAACATCAATTGTGTC+TGG 0.462911 4_3:+52237998 None:intergenic
TGCACAGGTTTCAGAAAAGA+AGG 0.467310 4_3:-52238632 Msa0646470:intron
TTTCAGGGCATCCTTTGCTA+TGG 0.470505 4_3:+52238522 None:intergenic
CTAGGCTTTGGAATCTCTCC+TGG 0.472910 4_3:+52239105 None:intergenic
TAGGAGTTGCTTTCTATCGT+GGG 0.475937 4_3:-52239085 Msa0646470:CDS
TGGCAGATGTCTCAAAATAT+GGG 0.483704 4_3:+52238578 None:intergenic
TTTGAGACATCTGCCAAAGA+AGG 0.487428 4_3:-52238571 Msa0646470:CDS
TTTCAAGAGCGTTCTTCTAC+GGG 0.490904 4_3:+52241248 None:intergenic
GGAAAATTCTCTGGATCGGA+AGG 0.495526 4_3:+52238836 None:intergenic
CGGGCATGGTGTGCAGCAAA+AGG 0.496942 4_3:-52238607 Msa0646470:CDS
CAACATCAATTGTGTCTGGC+AGG 0.497094 4_3:+52238002 None:intergenic
CAATGTTGGAATAGCATACA+AGG 0.501772 4_3:+52237754 None:intergenic
TCTAGCCGTTGCTGTCTATG+AGG 0.502452 4_3:-52237876 Msa0646470:three_prime_UTR
TGCCCTGAAAAGTGGGGAAG+AGG 0.506394 4_3:-52238509 Msa0646470:CDS
GGAAACAAGATCGACATTGA+TGG 0.507185 4_3:-52238800 Msa0646470:CDS
GATTTGGGATACAGCTGGCC+AGG 0.507917 4_3:-52239123 Msa0646470:CDS
GGTTAAGAAGTCAGCTCCAA+TGG 0.508254 4_3:+52240159 None:intergenic
TCATCATTCTCGGCGACAGC+GGG 0.509207 4_3:-52241224 Msa0646470:intron
CTAGGAGTTGCTTTCTATCG+TGG 0.516699 4_3:-52239086 Msa0646470:CDS
AATCAGTACAAGGCAACCAT+TGG 0.525698 4_3:-52240175 Msa0646470:CDS
GCACCATTCACAAACCTGTA+AGG 0.531833 4_3:+52240105 None:intergenic
TTGCAGATTTGGGATACAGC+TGG 0.533863 4_3:-52239128 Msa0646470:intron
CAAAGGATGCCCTGAAAAGT+GGG 0.534379 4_3:-52238516 Msa0646470:CDS
AGGTTTCAGAAAAGAAGGCT+CGG 0.540140 4_3:-52238627 Msa0646470:CDS
AAAAGGAAAATTCTCTGGAT+CGG 0.543661 4_3:+52238832 None:intergenic
CTGCCAGACACAATTGATGT+TGG 0.557538 4_3:-52238001 Msa0646470:CDS
GGTTTCAGAAAAGAAGGCTC+GGG 0.559631 4_3:-52238626 Msa0646470:CDS
CACTCACATCCTGATGACCT+TGG 0.564551 4_3:+52237965 None:intergenic
CACGATAGAAAGCAACTCCT+AGG 0.569261 4_3:+52239087 None:intergenic
TGCATAGAAAGTTAGAGAGA+GGG 0.573221 4_3:-52241376 Msa0646470:five_prime_UTR
TTGACAACCTTAACAACTGG+AGG 0.575421 4_3:-52239016 Msa0646470:CDS
CAGGAGAGATTCCAAAGCCT+AGG 0.576665 4_3:-52239104 Msa0646470:CDS
AACAAGATCGACATTGATGG+TGG 0.578098 4_3:-52238797 Msa0646470:CDS
AAGAGCGTTCTTCTACGGGA+AGG 0.590281 4_3:+52241252 None:intergenic
AGCATTCCAAACCATAGCAA+AGG 0.595720 4_3:-52238533 Msa0646470:CDS
CAGAAAAGAAGGCTCGGGCA+TGG 0.609079 4_3:-52238621 Msa0646470:CDS
CGGGAAGGCATCGTTCAGAT+CGG 0.609490 4_3:+52241267 None:intergenic
GTCATCATTCTCGGCGACAG+CGG 0.612250 4_3:-52241225 Msa0646470:CDS
AGGAGTTGCTTTCTATCGTG+GGG 0.614079 4_3:-52239084 Msa0646470:CDS
GAAGTTTAGTAATCAGTACA+AGG 0.614358 4_3:-52240185 Msa0646470:CDS
CTGCATAGAAAGTTAGAGAG+AGG 0.614439 4_3:-52241377 Msa0646470:five_prime_UTR
GAGGGAAACAGAGTTGAAGA+CGG 0.638749 4_3:-52241358 Msa0646470:five_prime_UTR
TTGGAAACAGCAATCAGCCA+AGG 0.645424 4_3:-52237982 Msa0646470:CDS
ACAAGATCGACATTGATGGT+GGG 0.651715 4_3:-52238796 Msa0646470:CDS
TGACAACCTTAACAACTGGA+GGG 0.655521 4_3:-52239015 Msa0646470:CDS
TGATGGTGGGAACAGTAGAG+TGG 0.665695 4_3:-52238783 Msa0646470:intron
TGAAACCTCATAGACAGCAA+CGG 0.678649 4_3:+52237871 None:intergenic
CAACCTTAACAACTGGAGGG+AGG 0.688480 4_3:-52239012 Msa0646470:CDS
TCACAAAACACATACCAAAG+CGG 0.704022 4_3:-52241302 Msa0646470:five_prime_UTR
AAAGGATGCCCTGAAAAGTG+GGG 0.713593 4_3:-52238515 Msa0646470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGATAATTATGATTAT+CGG + chr4_3:52237918-52237937 None:intergenic 10.0%
!! ACTTAATTTAAAAAATTGTT+TGG - chr4_3:52238388-52238407 Msa0646470:intron 10.0%
!!! TATTGAAATTTAAGTTAATA+TGG - chr4_3:52239887-52239906 Msa0646470:intron 10.0%
!!! ATATTATTTTAAAATCACTT+TGG - chr4_3:52241180-52241199 Msa0646470:intron 10.0%
!!! TTCACATATCTAAAATATAT+AGG + chr4_3:52238875-52238894 None:intergenic 15.0%
!!! GTACAAAATAATTTTTGTAT+GGG + chr4_3:52241410-52241429 None:intergenic 15.0%
!!! TGTACAAAATAATTTTTGTA+TGG + chr4_3:52241411-52241430 None:intergenic 15.0%
!! CTATAAGTAACTTAATTTAC+AGG - chr4_3:52238081-52238100 Msa0646470:intron 20.0%
!! AAATCAACTTAACATTAACA+TGG + chr4_3:52238424-52238443 None:intergenic 20.0%
!! AGATAAACATTCCAAAAAAA+GGG + chr4_3:52239094-52239113 None:intergenic 20.0%
!! AAGATAAACATTCCAAAAAA+AGG + chr4_3:52239095-52239114 None:intergenic 20.0%
!! ACATATAGATTGAATCAAAT+GGG - chr4_3:52239607-52239626 Msa0646470:intron 20.0%
!!! AATTTTCCTTTTGTTGTTAT+AGG - chr4_3:52240262-52240281 Msa0646470:intron 20.0%
!! CTTCATAAAAAATGCATTAA+GGG - chr4_3:52240646-52240665 Msa0646470:intron 20.0%
!! CAAAAAGATTCATAATCTAT+GGG + chr4_3:52240966-52240985 None:intergenic 20.0%
!!! ATCTTTTTGCTATTTGATAA+TGG - chr4_3:52240977-52240996 Msa0646470:intron 20.0%
!!! TCTTTTTGCTATTTGATAAT+GGG - chr4_3:52240978-52240997 Msa0646470:intron 20.0%
! GAAGAATAAAAATAAGCAGA+AGG + chr4_3:52237966-52237985 None:intergenic 25.0%
!!! TCATTTTTCTTATTCTTGTC+TGG - chr4_3:52238042-52238061 Msa0646470:intron 25.0%
!!! GAATTATTTGTTGTATCTAC+AGG - chr4_3:52238451-52238470 Msa0646470:intron 25.0%
! TTTGTACTAAATAGCTATTG+AGG + chr4_3:52238657-52238676 None:intergenic 25.0%
! TACAAAATACTGTGAAATAG+CGG - chr4_3:52238671-52238690 Msa0646470:intron 25.0%
!!! TGAGGTTTTAGTATTGTATA+GGG - chr4_3:52238810-52238829 Msa0646470:CDS 25.0%
!!! GGTAATGCTTTAAAAATATG+CGG - chr4_3:52238831-52238850 Msa0646470:CDS 25.0%
! TCAAAATCAAATTATGCACA+AGG + chr4_3:52239006-52239025 None:intergenic 25.0%
!!! AAATTACCAAACCCTTTTTT+TGG - chr4_3:52239080-52239099 Msa0646470:CDS 25.0%
! TCTTTCTGATATTACATAGA+TGG - chr4_3:52239111-52239130 Msa0646470:CDS 25.0%
! GATATTACATAGATGGTTTA+TGG - chr4_3:52239118-52239137 Msa0646470:CDS 25.0%
! GATTTAATTGAAGAATTCGT+AGG - chr4_3:52239239-52239258 Msa0646470:intron 25.0%
!!! ATCCTAAAGTTTGCATTTTT+TGG - chr4_3:52239396-52239415 Msa0646470:intron 25.0%
! TTCCAAAAAATGCAAACTTT+AGG + chr4_3:52239401-52239420 None:intergenic 25.0%
!!! AAGTTTGCATTTTTTGGAAA+CGG - chr4_3:52239402-52239421 Msa0646470:intron 25.0%
! AACAATTTCTCAAAAATGAC+AGG + chr4_3:52239466-52239485 None:intergenic 25.0%
! GACATATAGATTGAATCAAA+TGG - chr4_3:52239606-52239625 Msa0646470:intron 25.0%
! CATATAGATTGAATCAAATG+GGG - chr4_3:52239608-52239627 Msa0646470:intron 25.0%
!!! TGTTAGGCTGTTTAATTTTT+TGG - chr4_3:52239731-52239750 Msa0646470:intron 25.0%
! ATTCTAAATCTTGCAGATTT+GGG - chr4_3:52239945-52239964 Msa0646470:intron 25.0%
! TTGTTTCCTATAACAACAAA+AGG + chr4_3:52240271-52240290 None:intergenic 25.0%
! AAACATGTTAGATAGCAAAA+GGG + chr4_3:52240338-52240357 None:intergenic 25.0%
! GCTTCATAAAAAATGCATTA+AGG - chr4_3:52240645-52240664 Msa0646470:intron 25.0%
! ATAGGATTGATGTTTAATGA+TGG - chr4_3:52240771-52240790 Msa0646470:intron 25.0%
! ATTAAACATCAATCCTAACA+TGG + chr4_3:52240817-52240836 None:intergenic 25.0%
! TTAGGATTGATGTTTAATGA+TGG - chr4_3:52240819-52240838 Msa0646470:intron 25.0%
! TCAATCTTTGCATTATAACA+TGG + chr4_3:52240865-52240884 None:intergenic 25.0%
! TAATGCAAAGATTGATATGT+TGG - chr4_3:52240870-52240889 Msa0646470:intron 25.0%
! GCAAAAAGATTCATAATCTA+TGG + chr4_3:52240967-52240986 None:intergenic 25.0%
! AAACATAACATCAGTAACAT+TGG + chr4_3:52241018-52241037 None:intergenic 25.0%
!! ATGTTACTGATGTTATGTTT+TGG - chr4_3:52241018-52241037 Msa0646470:intron 25.0%
!! TGTTACTGATGTTATGTTTT+GGG - chr4_3:52241019-52241038 Msa0646470:intron 25.0%
! ATTGTGAAAAAAAACAGCAA+AGG - chr4_3:52241348-52241367 Msa0646470:five_prime_UTR 25.0%
TCTCTCTATCGTAAATCTTA+AGG + chr4_3:52237678-52237697 None:intergenic 30.0%
TAGATAACAAGAGTCATATC+TGG - chr4_3:52238123-52238142 Msa0646470:intron 30.0%
TATAGAGTAGCTTAATGCAT+AGG - chr4_3:52238316-52238335 Msa0646470:intron 30.0%
! GTATCCTTCGATTTTTGTAT+AGG - chr4_3:52238556-52238575 Msa0646470:CDS 30.0%
ACTTCCTATACAAAAATCGA+AGG + chr4_3:52238563-52238582 None:intergenic 30.0%
! ATTTTCACTGCAATTTTCTG+AGG - chr4_3:52238792-52238811 Msa0646470:CDS 30.0%
!! CTGAGGTTTTAGTATTGTAT+AGG - chr4_3:52238809-52238828 Msa0646470:CDS 30.0%
GAAGTTTAGTAATCAGTACA+AGG - chr4_3:52238898-52238917 Msa0646470:intron 30.0%
ATCTTCAAATTGCACTTCTT+TGG + chr4_3:52238948-52238967 None:intergenic 30.0%
! AAGAAGTGCAATTTGAAGAT+AGG - chr4_3:52238948-52238967 Msa0646470:intron 30.0%
! GGTTTGGTAATTTGTAAATC+TGG + chr4_3:52239073-52239092 None:intergenic 30.0%
AACATTCCAAAAAAAGGGTT+TGG + chr4_3:52239089-52239108 None:intergenic 30.0%
ATGTTCAACCTTCATTTAAG+AGG + chr4_3:52239271-52239290 None:intergenic 30.0%
ACTTAATACAGTAAACTACC+AGG + chr4_3:52239338-52239357 None:intergenic 30.0%
!!! AGTTTGCATTTTTTGGAAAC+GGG - chr4_3:52239403-52239422 Msa0646470:intron 30.0%
!! TATATTTTTGACGATTGCCT+TGG - chr4_3:52239489-52239508 Msa0646470:intron 30.0%
TACATACATGTGCAAACTAA+CGG + chr4_3:52239550-52239569 None:intergenic 30.0%
TAACAATCCTTAACCGAAAA+AGG + chr4_3:52239716-52239735 None:intergenic 30.0%
!! TTTTTCGGTTAAGGATTGTT+AGG - chr4_3:52239715-52239734 Msa0646470:intron 30.0%
!!! ATTTTTTGGACAGTACACTT+TGG - chr4_3:52239745-52239764 Msa0646470:intron 30.0%
TTTCCACAAGAAACAAAACA+TGG + chr4_3:52239822-52239841 None:intergenic 30.0%
CATTCTAAATCTTGCAGATT+TGG - chr4_3:52239944-52239963 Msa0646470:intron 30.0%
AAAAGGAAAATTCTCTGGAT+CGG + chr4_3:52240254-52240273 None:intergenic 30.0%
ACAACAAAAGGAAAATTCTC+TGG + chr4_3:52240259-52240278 None:intergenic 30.0%
GAAACATGTTAGATAGCAAA+AGG + chr4_3:52240339-52240358 None:intergenic 30.0%
CTGTTTAGCAGTACATATTT+AGG + chr4_3:52240410-52240429 None:intergenic 30.0%
TTCGACATTAATACCTTCTT+TGG + chr4_3:52240528-52240547 None:intergenic 30.0%
TCTTGGAAACAAATGCATAT+AGG - chr4_3:52240753-52240772 Msa0646470:intron 30.0%
TTGCATTATAACATGGCAAA+TGG + chr4_3:52240858-52240877 None:intergenic 30.0%
!! TTGGTTTGTCCATATAATAG+TGG - chr4_3:52240889-52240908 Msa0646470:intron 30.0%
!!! ATTGTTTTAGTGCGATTTGT+TGG - chr4_3:52241264-52241283 Msa0646470:exon 30.0%
!!! TTGTTTTAGTGCGATTTGTT+GGG - chr4_3:52241265-52241284 Msa0646470:exon 30.0%
!!! GCTGTTTTTTTTCACAATGT+TGG + chr4_3:52241346-52241365 None:intergenic 30.0%
TGCATAGAAAGTTAGAGAGA+GGG - chr4_3:52237707-52237726 Msa0646470:three_prime_UTR 35.0%
TCACAAAACACATACCAAAG+CGG - chr4_3:52237781-52237800 Msa0646470:three_prime_UTR 35.0%
CTCTTGAAAGTCATCATTCT+CGG - chr4_3:52237849-52237868 Msa0646470:three_prime_UTR 35.0%
TCTCGAACTCTTCAATTCTA+GGG - chr4_3:52237888-52237907 Msa0646470:three_prime_UTR 35.0%
TTGACATGCAAGCTCAAAAT+TGG - chr4_3:52238008-52238027 Msa0646470:CDS 35.0%
!! TAGTGTTGTTGATGATTTGC+AGG - chr4_3:52238507-52238526 Msa0646470:CDS 35.0%
!! AGTGTTGTTGATGATTTGCA+GGG - chr4_3:52238508-52238527 Msa0646470:CDS 35.0%
AGGAAGTCAGTATTATGTTC+AGG - chr4_3:52238576-52238595 Msa0646470:CDS 35.0%
GGAAGTCAGTATTATGTTCA+GGG - chr4_3:52238577-52238596 Msa0646470:CDS 35.0%
CGAATAGTACTATTGCATAG+CGG - chr4_3:52238608-52238627 Msa0646470:CDS 35.0%
! GCTATAACACTTTTGCATAG+CGG - chr4_3:52238704-52238723 Msa0646470:intron 35.0%
! AATCTGGCCTTTCATTTACA+AGG + chr4_3:52239057-52239076 None:intergenic 35.0%
! AAGCCTTTTCCAACTAAATG+GGG + chr4_3:52239167-52239186 None:intergenic 35.0%
! CAAGCCTTTTCCAACTAAAT+GGG + chr4_3:52239168-52239187 None:intergenic 35.0%
!! AAATGCAAACTTTAGGATGC+TGG + chr4_3:52239394-52239413 None:intergenic 35.0%
!!! CATTTTAAAGTCATGTGCCA+AGG + chr4_3:52239509-52239528 None:intergenic 35.0%
! CTAATACTGAAAGAGCATGA+CGG + chr4_3:52239795-52239814 None:intergenic 35.0%
!!! CATCCATGTTTTGTTTCTTG+TGG - chr4_3:52239816-52239835 Msa0646470:intron 35.0%
CATTTGACAACCTTAACAAC+TGG - chr4_3:52240064-52240083 Msa0646470:intron 35.0%
AACTTTATGATCACTGCATC+AGG - chr4_3:52240219-52240238 Msa0646470:intron 35.0%
AGGTGTTTCATGAATGTATC+TGG + chr4_3:52240390-52240409 None:intergenic 35.0%
TGGCAGATGTCTCAAAATAT+GGG + chr4_3:52240508-52240527 None:intergenic 35.0%
TTGGCAGATGTCTCAAAATA+TGG + chr4_3:52240509-52240528 None:intergenic 35.0%
GGGTATATCTGATTATGAGA+TGG - chr4_3:52240666-52240685 Msa0646470:intron 35.0%
GATTATGAGATGGTGTTCAT+TGG - chr4_3:52240676-52240695 Msa0646470:intron 35.0%
AGTGAACTAAAACAACTAGC+AGG + chr4_3:52240722-52240741 None:intergenic 35.0%
TTCACTATATCCATGTCTCT+TGG - chr4_3:52240736-52240755 Msa0646470:intron 35.0%
ATCAATCCTAACATGGCAAA+TGG + chr4_3:52240810-52240829 None:intergenic 35.0%
TTTCCAACATCAATTGTGTC+TGG + chr4_3:52241088-52241107 None:intergenic 35.0%
CAATGTTGGAATAGCATACA+AGG + chr4_3:52241332-52241351 None:intergenic 35.0%
CTGCATAGAAAGTTAGAGAG+AGG - chr4_3:52237706-52237725 Msa0646470:three_prime_UTR 40.0%
!! TTTCAAGAGCGTTCTTCTAC+GGG + chr4_3:52237838-52237857 None:intergenic 40.0%
!! CTTTCAAGAGCGTTCTTCTA+CGG + chr4_3:52237839-52237858 None:intergenic 40.0%
CTCTCGAACTCTTCAATTCT+AGG - chr4_3:52237887-52237906 Msa0646470:three_prime_UTR 40.0%
ACATCTTCGGTCAAAAGTCT+CGG - chr4_3:52238281-52238300 Msa0646470:intron 40.0%
!! TTGTTGATGATTTGCAGGGT+GGG - chr4_3:52238512-52238531 Msa0646470:CDS 40.0%
ATACTGTGAAATAGCGGCTA+TGG - chr4_3:52238677-52238696 Msa0646470:intron 40.0%
AATCAGTACAAGGCAACCAT+TGG - chr4_3:52238908-52238927 Msa0646470:intron 40.0%
! AGATAGGCTTTTCACCTTAC+AGG - chr4_3:52238964-52238983 Msa0646470:intron 40.0%
TCACCTTACAGGTTTGTGAA+TGG - chr4_3:52238975-52238994 Msa0646470:intron 40.0%
! TTTACAAGGCTTGGTCATGA+AGG + chr4_3:52239043-52239062 None:intergenic 40.0%
! GACCAAGCCTTGTAAATGAA+AGG - chr4_3:52239047-52239066 Msa0646470:CDS 40.0%
! CTTTTCCAACTAAATGGGGT+CGG + chr4_3:52239163-52239182 None:intergenic 40.0%
! CCAAGCCTTTTCCAACTAAA+TGG + chr4_3:52239169-52239188 None:intergenic 40.0%
CCATTTAGTTGGAAAAGGCT+TGG - chr4_3:52239166-52239185 Msa0646470:intron 40.0%
GGTCACAACCTCTTAAATGA+AGG - chr4_3:52239260-52239279 Msa0646470:intron 40.0%
ACATGTGCAAACTAACGGTT+AGG + chr4_3:52239545-52239564 None:intergenic 40.0%
! AGTTTTTCCGTTGCACTCTT+AGG - chr4_3:52239688-52239707 Msa0646470:intron 40.0%
!!! TTAGGAGCCTTTTTCGGTTA+AGG - chr4_3:52239706-52239725 Msa0646470:intron 40.0%
! TTTTGGACAGTACACTTTGG+TGG - chr4_3:52239748-52239767 Msa0646470:intron 40.0%
TTTGGACAGTACACTTTGGT+GGG - chr4_3:52239749-52239768 Msa0646470:intron 40.0%
! TAGGAGTTGCTTTCTATCGT+GGG - chr4_3:52239998-52240017 Msa0646470:intron 40.0%
TTGACAACCTTAACAACTGG+AGG - chr4_3:52240067-52240086 Msa0646470:intron 40.0%
TGACAACCTTAACAACTGGA+GGG - chr4_3:52240068-52240087 Msa0646470:intron 40.0%
GGAAACAAGATCGACATTGA+TGG - chr4_3:52240283-52240302 Msa0646470:intron 40.0%
AACAAGATCGACATTGATGG+TGG - chr4_3:52240286-52240305 Msa0646470:intron 40.0%
! ACAAGATCGACATTGATGGT+GGG - chr4_3:52240287-52240306 Msa0646470:intron 40.0%
CATGTTAGATAGCAAAAGGG+AGG + chr4_3:52240335-52240354 None:intergenic 40.0%
ACACATAAGTTCTTGTGCAC+AGG - chr4_3:52240436-52240455 Msa0646470:intron 40.0%
TGCACAGGTTTCAGAAAAGA+AGG - chr4_3:52240451-52240470 Msa0646470:intron 40.0%
AGGTTTCAGAAAAGAAGGCT+CGG - chr4_3:52240456-52240475 Msa0646470:intron 40.0%
! TTTGAGACATCTGCCAAAGA+AGG - chr4_3:52240512-52240531 Msa0646470:intron 40.0%
AGCATTCCAAACCATAGCAA+AGG - chr4_3:52240550-52240569 Msa0646470:intron 40.0%
GCATTTGTTTCCAAGAGACA+TGG + chr4_3:52240749-52240768 None:intergenic 40.0%
CATGAACCATTTGCCATGTT+AGG - chr4_3:52240801-52240820 Msa0646470:intron 40.0%
CTTTGCACGCCACTATTATA+TGG + chr4_3:52240901-52240920 None:intergenic 40.0%
! TTTGGGATTTGTTCTCCATG+TGG - chr4_3:52241036-52241055 Msa0646470:intron 40.0%
AACAAGCACGAATTTCCACA+TGG + chr4_3:52241054-52241073 None:intergenic 40.0%
TGAAACCTCATAGACAGCAA+CGG + chr4_3:52241215-52241234 None:intergenic 40.0%
GAGGGAAACAGAGTTGAAGA+CGG - chr4_3:52237725-52237744 Msa0646470:three_prime_UTR 45.0%
GGATCAAGAGTTAACCGCTT+TGG + chr4_3:52237798-52237817 None:intergenic 45.0%
TCTGACACTGACACATGTGT+AGG + chr4_3:52238210-52238229 None:intergenic 45.0%
ATGTGTCAGTGTCAGACATG+CGG - chr4_3:52238214-52238233 Msa0646470:intron 45.0%
TTGGTCACTGGACACATCTT+CGG - chr4_3:52238268-52238287 Msa0646470:intron 45.0%
!! GTTGTTGATGATTTGCAGGG+TGG - chr4_3:52238511-52238530 Msa0646470:CDS 45.0%
!! TGTTGATGATTTGCAGGGTG+GGG - chr4_3:52238513-52238532 Msa0646470:CDS 45.0%
GGTTAAGAAGTCAGCTCCAA+TGG + chr4_3:52238927-52238946 None:intergenic 45.0%
GCACCATTCACAAACCTGTA+AGG + chr4_3:52238981-52239000 None:intergenic 45.0%
GGCCTTTCATTTACAAGGCT+TGG + chr4_3:52239052-52239071 None:intergenic 45.0%
ACTAAATGGGGTCGGATACA+TGG + chr4_3:52239155-52239174 None:intergenic 45.0%
TGTATCCGACCCCATTTAGT+TGG - chr4_3:52239155-52239174 Msa0646470:intron 45.0%
CGACCCCATTTAGTTGGAAA+AGG - chr4_3:52239161-52239180 Msa0646470:intron 45.0%
GGCGTGTTTAATGTACACAC+AGG + chr4_3:52239317-52239336 None:intergenic 45.0%
GTGTGTACATTAAACACGCC+TGG - chr4_3:52239317-52239336 Msa0646470:intron 45.0%
! TTTGGAAACGGGCTTGTGTA+TGG - chr4_3:52239414-52239433 Msa0646470:intron 45.0%
! TTGGAAACGGGCTTGTGTAT+GGG - chr4_3:52239415-52239434 Msa0646470:intron 45.0%
! AAAGGCTCCTAAGAGTGCAA+CGG + chr4_3:52239698-52239717 None:intergenic 45.0%
! GCACTCTTAGGAGCCTTTTT+CGG - chr4_3:52239700-52239719 Msa0646470:intron 45.0%
TTGGACAGTACACTTTGGTG+GGG - chr4_3:52239750-52239769 Msa0646470:intron 45.0%
! TTGCAGATTTGGGATACAGC+TGG - chr4_3:52239955-52239974 Msa0646470:intron 45.0%
AGAAAGCAACTCCTAGGCTT+TGG + chr4_3:52239993-52240012 None:intergenic 45.0%
CACGATAGAAAGCAACTCCT+AGG + chr4_3:52239999-52240018 None:intergenic 45.0%
! CTAGGAGTTGCTTTCTATCG+TGG - chr4_3:52239997-52240016 Msa0646470:intron 45.0%
! AGGAGTTGCTTTCTATCGTG+GGG - chr4_3:52239999-52240018 Msa0646470:intron 45.0%
ATTCCTCCCTCCAGTTGTTA+AGG + chr4_3:52240077-52240096 None:intergenic 45.0%
GGAAAATTCTCTGGATCGGA+AGG + chr4_3:52240250-52240269 None:intergenic 45.0%
GGTTTCAGAAAAGAAGGCTC+GGG - chr4_3:52240457-52240476 Msa0646470:intron 45.0%
! TTTCAGGGCATCCTTTGCTA+TGG + chr4_3:52240564-52240583 None:intergenic 45.0%
CAAAGGATGCCCTGAAAAGT+GGG - chr4_3:52240567-52240586 Msa0646470:intron 45.0%
AAAGGATGCCCTGAAAAGTG+GGG - chr4_3:52240568-52240587 Msa0646470:intron 45.0%
!! TTCCTCTTCCCCACTTTTCA+GGG + chr4_3:52240579-52240598 None:intergenic 45.0%
ATGAGATGGTGTTCATTGGC+TGG - chr4_3:52240680-52240699 Msa0646470:intron 45.0%
! TGAGATGGTGTTCATTGGCT+GGG - chr4_3:52240681-52240700 Msa0646470:intron 45.0%
CAACATCAATTGTGTCTGGC+AGG + chr4_3:52241084-52241103 None:intergenic 45.0%
CTGCCAGACACAATTGATGT+TGG - chr4_3:52241082-52241101 Msa0646470:intron 45.0%
TTGGAAACAGCAATCAGCCA+AGG - chr4_3:52241101-52241120 Msa0646470:intron 45.0%
!! CGATTTGTTGGGTGCTTGTT+TGG - chr4_3:52241276-52241295 Msa0646470:exon 45.0%
!! AAGAGCGTTCTTCTACGGGA+AGG + chr4_3:52237834-52237853 None:intergenic 50.0%
TGTGTCAGTGTCAGACATGC+GGG - chr4_3:52238215-52238234 Msa0646470:intron 50.0%
AGACATGCGGGACATTGTGT+AGG - chr4_3:52238227-52238246 Msa0646470:intron 50.0%
ACACGACACATGTTGGTCAC+TGG - chr4_3:52238256-52238275 Msa0646470:intron 50.0%
CTGTGAAATAGCGGCTATGG+CGG - chr4_3:52238680-52238699 Msa0646470:intron 50.0%
CGGGCTTGTGTATGGGATTT+AGG - chr4_3:52239422-52239441 Msa0646470:intron 50.0%
!! CTAGGCTTTGGAATCTCTCC+TGG + chr4_3:52239981-52240000 None:intergenic 50.0%
CAGGAGAGATTCCAAAGCCT+AGG - chr4_3:52239979-52239998 Msa0646470:intron 50.0%
CAACCTTAACAACTGGAGGG+AGG - chr4_3:52240071-52240090 Msa0646470:intron 50.0%
!! TGATGGTGGGAACAGTAGAG+TGG - chr4_3:52240300-52240319 Msa0646470:intron 50.0%
! GGGCATCCTTTGCTATGGTT+TGG + chr4_3:52240559-52240578 None:intergenic 50.0%
GCAAAGGATGCCCTGAAAAG+TGG - chr4_3:52240566-52240585 Msa0646470:intron 50.0%
!! CTTCCTCTTCCCCACTTTTC+AGG + chr4_3:52240580-52240599 None:intergenic 50.0%
! GAGATGGTGTTCATTGGCTG+GGG - chr4_3:52240682-52240701 Msa0646470:intron 50.0%
! AGATGGTGTTCATTGGCTGG+GGG - chr4_3:52240683-52240702 Msa0646470:intron 50.0%
!! TTGGCTGGGGGAGCATATTT+TGG - chr4_3:52240695-52240714 Msa0646470:intron 50.0%
AGCAATCAGCCAAGGTCATC+AGG - chr4_3:52241109-52241128 Msa0646470:intron 50.0%
CACTCACATCCTGATGACCT+TGG + chr4_3:52241121-52241140 None:intergenic 50.0%
TCTAGCCGTTGCTGTCTATG+AGG - chr4_3:52241207-52241226 Msa0646470:intron 50.0%
!! CGGGAAGGCATCGTTCAGAT+CGG + chr4_3:52237819-52237838 None:intergenic 55.0%
GTCATCATTCTCGGCGACAG+CGG - chr4_3:52237858-52237877 Msa0646470:three_prime_UTR 55.0%
TCATCATTCTCGGCGACAGC+GGG - chr4_3:52237859-52237878 Msa0646470:three_prime_UTR 55.0%
GACAGCGACACGACACATGT+TGG - chr4_3:52238249-52238268 Msa0646470:intron 55.0%
! GATTTGGGATACAGCTGGCC+AGG - chr4_3:52239960-52239979 Msa0646470:intron 55.0%
CAGAAAAGAAGGCTCGGGCA+TGG - chr4_3:52240462-52240481 Msa0646470:intron 55.0%
TGCCCTGAAAAGTGGGGAAG+AGG - chr4_3:52240574-52240593 Msa0646470:intron 55.0%
CGGGCATGGTGTGCAGCAAA+AGG - chr4_3:52240476-52240495 Msa0646470:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 52237667 52241438 52237667 ID=Msa0646470;Name=Msa0646470
chr4_3 mRNA 52237667 52241438 52237667 ID=Msa0646470-mRNA-1;Parent=Msa0646470;Name=Msa0646470-mRNA-1;_AED=0.08;_eAED=0.08;_QI=161|0.8|1|1|1|1|6|294|197
chr4_3 exon 52237667 52238027 52237667 ID=Msa0646470-mRNA-1:exon:12796;Parent=Msa0646470-mRNA-1
chr4_3 exon 52238502 52238647 52238502 ID=Msa0646470-mRNA-1:exon:12795;Parent=Msa0646470-mRNA-1
chr4_3 exon 52238784 52238864 52238784 ID=Msa0646470-mRNA-1:exon:12794;Parent=Msa0646470-mRNA-1
chr4_3 exon 52238998 52239144 52238998 ID=Msa0646470-mRNA-1:exon:12793;Parent=Msa0646470-mRNA-1
chr4_3 exon 52240120 52240219 52240120 ID=Msa0646470-mRNA-1:exon:12792;Parent=Msa0646470-mRNA-1
chr4_3 exon 52241225 52241438 52241225 ID=Msa0646470-mRNA-1:exon:12791;Parent=Msa0646470-mRNA-1
chr4_3 five_prime_UTR 52241278 52241438 52241278 ID=Msa0646470-mRNA-1:five_prime_utr;Parent=Msa0646470-mRNA-1
chr4_3 CDS 52241225 52241277 52241225 ID=Msa0646470-mRNA-1:cds;Parent=Msa0646470-mRNA-1
chr4_3 CDS 52240120 52240219 52240120 ID=Msa0646470-mRNA-1:cds;Parent=Msa0646470-mRNA-1
chr4_3 CDS 52238998 52239144 52238998 ID=Msa0646470-mRNA-1:cds;Parent=Msa0646470-mRNA-1
chr4_3 CDS 52238784 52238864 52238784 ID=Msa0646470-mRNA-1:cds;Parent=Msa0646470-mRNA-1
chr4_3 CDS 52238502 52238647 52238502 ID=Msa0646470-mRNA-1:cds;Parent=Msa0646470-mRNA-1
chr4_3 CDS 52237961 52238027 52237961 ID=Msa0646470-mRNA-1:cds;Parent=Msa0646470-mRNA-1
chr4_3 three_prime_UTR 52237667 52237960 52237667 ID=Msa0646470-mRNA-1:three_prime_utr;Parent=Msa0646470-mRNA-1
Gene Sequence

>Msa0646470

ATGCCTTCCCGTAGAAGAACGCTCTTGAAAGTCATCATTCTCGGCGACAGCGGATATGTGAATAAGAAGTTTAGTAATCAGTACAAGGCAACCATTGGAGCTGACTTCTTAACCAAAGAAGTGCAATTTGAAGATAGGCTTTTCACCTTACAGATTTGGGATACAGCTGGCCAGGAGAGATTCCAAAGCCTAGGAGTTGCTTTCTATCGTGGGGCTGATTGCTGTGTTCTTGTATATGATGTTAATTCAATGAAATCATTTGACAACCTTAACAACTGGAGGGAGGAATTTCTGATTCAAGCAAATCCTTCCGATCCAGAGAATTTTCCTTTTGTTGTTATAGGAAACAAGATCGACATTGATGGTGGGAACAGTAGAGTGGTTTCAGAAAAGAAGGCTCGGGCATGGTGTGCAGCAAAAGGAAATATCCCATATTTTGAGACATCTGCCAAAGAAGGTATTAATGTCGAAGAAGCATTCCAAACCATAGCAAAGGATGCCCTGAAAAGTGGGGAAGAGGAAGAATTATACCTGCCAGACACAATTGATGTTGGAAACAGCAATCAGCCAAGGTCATCAGGATGTGAGTGCTGA
Protein sequence

>Msa0646470

MPSRRRTLLKVIILGDSGYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQANPSDPENFPFVVIGNKIDIDGGNSRVVSEKKARAWCAAKGNIPYFETSAKEGINVEEAFQTIAKDALKSGEEEELYLPDTIDVGNSNQPRSSGCEC*