AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0254650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0254650 MtrunA17_Chr2g0315311 85.246 122 17 1 1 121 1 122 6.78e-61 182
Msa0254650 MtrunA17_Chr2g0315301 61.268 142 19 3 1 118 1 130 2.87e-46 146
Msa0254650 MtrunA17_Chr2g0315321 66.102 118 23 4 1 118 1 101 9.87e-32 108
Msa0254650 MtrunA17_Chr2g0315291 65.254 118 26 2 1 118 1 103 7.97e-26 94.0
Msa0254650 MtrunA17_Chr2g0315331 58.678 121 36 3 1 107 1 121 9.32e-25 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTTGTGCATGATTATT+TGG 0.069508 2_2:-48216861 Msa0254650:five_prime_UTR
TATCATAAGGACATTGTTTA+AGG 0.172598 2_2:+48215256 None:intergenic
TTCTTTGTGCATGATTATTT+GGG 0.272311 2_2:-48216860 Msa0254650:five_prime_UTR
AGCACAGGAAGCGGAGGATT+CGG 0.330833 2_2:-48215598 Msa0254650:CDS
AGCACAGGAAGCGGAGGATT+GGG 0.335111 2_2:-48215493 Msa0254650:CDS
GCACAGGAAGCGGAGGATTC+GGG 0.352203 2_2:-48215597 Msa0254650:CDS
TAGCACAGGAAGCGGAGGAT+TGG 0.389817 2_2:-48215494 Msa0254650:CDS
TTAAACAAACACACTAACTT+TGG 0.404636 2_2:-48216899 Msa0254650:five_prime_UTR
GGGTGGGCAGCACTGGCATG+TGG 0.407335 2_2:+48216772 None:intergenic
TATTTGAGGGTGGGCAGCAC+TGG 0.427483 2_2:+48216765 None:intergenic
ATAATTTGTAATATTTCAAA+TGG 0.428374 2_2:-48216817 Msa0254650:exon
CCGCTTCCTGTGCTACCCCT+TGG 0.433928 2_2:+48215607 None:intergenic
CATAACTATACAATGTCTTA+AGG 0.435385 2_2:+48215339 None:intergenic
ATCAGAGGTTTATGCGTTTG+CGG 0.444972 2_2:+48215463 None:intergenic
TAACCATCATTTGTCGATGT+TGG 0.450141 2_2:+48215568 None:intergenic
TTTCAAATGGCTCTTGCAGC+AGG 0.451956 2_2:-48216804 Msa0254650:exon
GGTGGATTTGTTGGTATCAG+AGG 0.464198 2_2:+48215448 None:intergenic
CAAAGTATCAAAGTATCATA+AGG 0.482373 2_2:+48215243 None:intergenic
GGTGGGCAGCACTGGCATGT+GGG 0.487384 2_2:+48216773 None:intergenic
CCGCTTCCTGTGCTACCCGT+TGG 0.493161 2_2:+48215502 None:intergenic
GATCCAACATCGACAAATGA+TGG 0.518991 2_2:-48215571 Msa0254650:CDS
CCAAGGGGTAGCACAGGAAG+CGG 0.530525 2_2:-48215607 Msa0254650:CDS
GCACAGGAAGCGGAGGATTG+GGG 0.533668 2_2:-48215492 Msa0254650:CDS
ATGAAACCAAGGGGTAGCAC+AGG 0.569869 2_2:-48215613 Msa0254650:CDS
CCAACGGGTAGCACAGGAAG+CGG 0.577109 2_2:-48215502 Msa0254650:CDS
TTTAGACGCATATTTGAGGG+TGG 0.587811 2_2:+48216755 None:intergenic
AGTTTATGCAATGAAACCAA+GGG 0.599375 2_2:-48215623 Msa0254650:CDS
TTAGACGCATATTTGAGGGT+GGG 0.609382 2_2:+48216756 None:intergenic
AAGTTTATGCAATGAAACCA+AGG 0.615778 2_2:-48215624 Msa0254650:CDS
TCTTTGTGCATGATTATTTG+GGG 0.629539 2_2:-48216859 Msa0254650:five_prime_UTR
AGGGGTAGCACAGGAAGCGG+AGG 0.630133 2_2:-48215604 Msa0254650:CDS
ACGGGTAGCACAGGAAGCGG+AGG 0.642552 2_2:-48215499 Msa0254650:CDS
GATGAAGATCAACTTGACAA+TGG 0.661313 2_2:-48215409 Msa0254650:CDS
TGACAATGGCAATGAGTGAG+TGG 0.667796 2_2:-48215395 Msa0254650:exon
AGGAAGCGGAGGATTCGGGA+AGG 0.688019 2_2:-48215593 Msa0254650:CDS
GTTTATGCAATGAAACCAAG+GGG 0.696629 2_2:-48215622 Msa0254650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATAAATTTAAATTTTT+TGG - chr2_2:48215650-48215669 Msa0254650:CDS 0.0%
!!! TTTATAAATTTAAATTTTTT+GGG - chr2_2:48215651-48215670 Msa0254650:CDS 0.0%
!!! TTTTATAAATTTAAATTTTT+TGG - chr2_2:48215650-48215669 Msa0254650:CDS 0.0%
!!! TTTATAAATTTAAATTTTTT+GGG - chr2_2:48215651-48215670 Msa0254650:CDS 0.0%
!!! ATAATTTGTAATATTTCAAA+TGG - chr2_2:48215299-48215318 Msa0254650:three_prime_UTR 10.0%
!!! TTTAAGGAATTTAAAATTTT+AGG - chr2_2:48215533-48215552 Msa0254650:CDS 10.0%
!! CAAATTTCAAAAAATTTAAA+AGG - chr2_2:48215708-48215727 Msa0254650:intron 10.0%
!!! TTATAATTTTGGAAATTTAT+TGG - chr2_2:48215736-48215755 Msa0254650:intron 10.0%
!!! TATAATTTTGGAAATTTATT+GGG - chr2_2:48215737-48215756 Msa0254650:intron 10.0%
!!! ATTTTAAATTCTTAACTTTT+AGG - chr2_2:48215829-48215848 Msa0254650:intron 10.0%
!!! ATAATTTGTAATATTTCAAA+TGG - chr2_2:48215299-48215318 Msa0254650:three_prime_UTR 10.0%
!!! TTTAAGGAATTTAAAATTTT+AGG - chr2_2:48215533-48215552 Msa0254650:CDS 10.0%
!! CAAATTTCAAAAAATTTAAA+AGG - chr2_2:48215708-48215727 Msa0254650:intron 10.0%
!!! TTATAATTTTGGAAATTTAT+TGG - chr2_2:48215736-48215755 Msa0254650:intron 10.0%
!!! TATAATTTTGGAAATTTATT+GGG - chr2_2:48215737-48215756 Msa0254650:intron 10.0%
!!! ATTTTAAATTCTTAACTTTT+AGG - chr2_2:48215829-48215848 Msa0254650:intron 10.0%
!! ATATCAAATATATACTTACA+TGG + chr2_2:48215403-48215422 None:intergenic 15.0%
!!! TATTTCTAAATATTCTTGTT+TGG - chr2_2:48215585-48215604 Msa0254650:CDS 15.0%
!!! AATTCATTTTCATCATTTTT+AGG - chr2_2:48215623-48215642 Msa0254650:CDS 15.0%
!!! AAAAGGTCAATTTATAATTT+TGG - chr2_2:48215725-48215744 Msa0254650:intron 15.0%
!!! ATAATTTTGGAAATTTATTG+GGG - chr2_2:48215738-48215757 Msa0254650:intron 15.0%
!!! TGAAAATTTTGGAAAATTTA+AGG - chr2_2:48215767-48215786 Msa0254650:intron 15.0%
!!! AATTAGTTTCTTAATTAAAC+TGG + chr2_2:48216356-48216375 None:intergenic 15.0%
!! ATAGATTTATAATCATTCAT+TGG - chr2_2:48216384-48216403 Msa0254650:intron 15.0%
!! ATATCAAATATATACTTACA+TGG + chr2_2:48215403-48215422 None:intergenic 15.0%
!!! TATTTCTAAATATTCTTGTT+TGG - chr2_2:48215585-48215604 Msa0254650:CDS 15.0%
!!! AATTCATTTTCATCATTTTT+AGG - chr2_2:48215623-48215642 Msa0254650:CDS 15.0%
!!! AAAAGGTCAATTTATAATTT+TGG - chr2_2:48215725-48215744 Msa0254650:intron 15.0%
!!! ATAATTTTGGAAATTTATTG+GGG - chr2_2:48215738-48215757 Msa0254650:intron 15.0%
!!! TGAAAATTTTGGAAAATTTA+AGG - chr2_2:48215767-48215786 Msa0254650:intron 15.0%
!!! AATTAGTTTCTTAATTAAAC+TGG + chr2_2:48216356-48216375 None:intergenic 15.0%
!! ATAGATTTATAATCATTCAT+TGG - chr2_2:48216384-48216403 Msa0254650:intron 15.0%
!! AATGACAACTTTGATTAAAT+AGG - chr2_2:48215424-48215443 Msa0254650:CDS 20.0%
!! ATGACAACTTTGATTAAATA+GGG - chr2_2:48215425-48215444 Msa0254650:CDS 20.0%
!!! ATTTTGAAAATTGAAAGTAG+AGG - chr2_2:48215677-48215696 Msa0254650:intron 20.0%
!!! TTTAGAAGGAGAATATTTTT+AGG + chr2_2:48215991-48216010 None:intergenic 20.0%
!! AATGACAACTTTGATTAAAT+AGG - chr2_2:48215424-48215443 Msa0254650:CDS 20.0%
!! ATGACAACTTTGATTAAATA+GGG - chr2_2:48215425-48215444 Msa0254650:CDS 20.0%
!!! ATTTTGAAAATTGAAAGTAG+AGG - chr2_2:48215677-48215696 Msa0254650:intron 20.0%
!!! TTTAGAAGGAGAATATTTTT+AGG + chr2_2:48215991-48216010 None:intergenic 20.0%
! TTCTTTGTGCATGATTATTT+GGG - chr2_2:48215256-48215275 Msa0254650:three_prime_UTR 25.0%
!!! TTTTTTAGACGCATATTTGA+GGG + chr2_2:48215367-48215386 None:intergenic 25.0%
!!! ATTTTTTAGACGCATATTTG+AGG + chr2_2:48215368-48215387 None:intergenic 25.0%
!!! CATTTTTTTAAGTAGTCTAG+TGG - chr2_2:48215462-48215481 Msa0254650:CDS 25.0%
!! GAGGTAATTGACAAATTTTA+AGG - chr2_2:48215517-48215536 Msa0254650:CDS 25.0%
!! TCTAAATATTCTTGTTTGGA+TGG - chr2_2:48215589-48215608 Msa0254650:CDS 25.0%
!!! CCATTGTTTTGATGATAATT+TGG + chr2_2:48215925-48215944 None:intergenic 25.0%
! CCAAATTATCATCAAAACAA+TGG - chr2_2:48215922-48215941 Msa0254650:intron 25.0%
! ATTGAAAAGATTACCAACTT+CGG - chr2_2:48216169-48216188 Msa0254650:intron 25.0%
!! TTTTCGCTTATAATAATGAC+CGG - chr2_2:48216251-48216270 Msa0254650:intron 25.0%
! AGTTAATCCTAAAAAACCAA+CGG - chr2_2:48216598-48216617 Msa0254650:intron 25.0%
!!! TCATCATCATCAGAATTTTT+TGG + chr2_2:48216692-48216711 None:intergenic 25.0%
! CATAACTATACAATGTCTTA+AGG + chr2_2:48216780-48216799 None:intergenic 25.0%
! TATCATAAGGACATTGTTTA+AGG + chr2_2:48216863-48216882 None:intergenic 25.0%
!! CAAAGTATCAAAGTATCATA+AGG + chr2_2:48216876-48216895 None:intergenic 25.0%
! TTCTTTGTGCATGATTATTT+GGG - chr2_2:48215256-48215275 Msa0254650:three_prime_UTR 25.0%
!!! TTTTTTAGACGCATATTTGA+GGG + chr2_2:48215367-48215386 None:intergenic 25.0%
!!! ATTTTTTAGACGCATATTTG+AGG + chr2_2:48215368-48215387 None:intergenic 25.0%
!!! CATTTTTTTAAGTAGTCTAG+TGG - chr2_2:48215462-48215481 Msa0254650:CDS 25.0%
!! GAGGTAATTGACAAATTTTA+AGG - chr2_2:48215517-48215536 Msa0254650:CDS 25.0%
!! TCTAAATATTCTTGTTTGGA+TGG - chr2_2:48215589-48215608 Msa0254650:CDS 25.0%
!!! CCATTGTTTTGATGATAATT+TGG + chr2_2:48215925-48215944 None:intergenic 25.0%
! CCAAATTATCATCAAAACAA+TGG - chr2_2:48215922-48215941 Msa0254650:intron 25.0%
! ATTGAAAAGATTACCAACTT+CGG - chr2_2:48216169-48216188 Msa0254650:intron 25.0%
!! TTTTCGCTTATAATAATGAC+CGG - chr2_2:48216251-48216270 Msa0254650:intron 25.0%
! AGTTAATCCTAAAAAACCAA+CGG - chr2_2:48216598-48216617 Msa0254650:intron 25.0%
!!! TCATCATCATCAGAATTTTT+TGG + chr2_2:48216692-48216711 None:intergenic 25.0%
! CATAACTATACAATGTCTTA+AGG + chr2_2:48216780-48216799 None:intergenic 25.0%
! TATCATAAGGACATTGTTTA+AGG + chr2_2:48216863-48216882 None:intergenic 25.0%
!! CAAAGTATCAAAGTATCATA+AGG + chr2_2:48216876-48216895 None:intergenic 25.0%
CTTCTTTGTGCATGATTATT+TGG - chr2_2:48215255-48215274 Msa0254650:three_prime_UTR 30.0%
TCTTTGTGCATGATTATTTG+GGG - chr2_2:48215257-48215276 Msa0254650:three_prime_UTR 30.0%
TTCAACTCTTAAAGTGTGTT+TGG - chr2_2:48215492-48215511 Msa0254650:CDS 30.0%
! TGGGGTCAATTTGAAAATTT+TGG - chr2_2:48215756-48215775 Msa0254650:intron 30.0%
! TTAGGTGACGTAATATTTTG+AGG + chr2_2:48215973-48215992 None:intergenic 30.0%
! CATGTGAACAAAGATTAGTT+GGG + chr2_2:48216085-48216104 None:intergenic 30.0%
AAGTTTATGCAATGAAACCA+AGG - chr2_2:48216492-48216511 Msa0254650:intron 30.0%
AGTTTATGCAATGAAACCAA+GGG - chr2_2:48216493-48216512 Msa0254650:intron 30.0%
GTTAATCCTAAAAAACCAAC+GGG - chr2_2:48216599-48216618 Msa0254650:intron 30.0%
!!! GAATTTTTTGGTGGATTTGT+TGG + chr2_2:48216680-48216699 None:intergenic 30.0%
!!! TCATCATCAGAATTTTTTGG+TGG + chr2_2:48216689-48216708 None:intergenic 30.0%
! TGAGTGGTGATTTTTCTTTT+TGG - chr2_2:48216737-48216756 Msa0254650:CDS 30.0%
CTTCTTTGTGCATGATTATT+TGG - chr2_2:48215255-48215274 Msa0254650:three_prime_UTR 30.0%
TCTTTGTGCATGATTATTTG+GGG - chr2_2:48215257-48215276 Msa0254650:three_prime_UTR 30.0%
TTCAACTCTTAAAGTGTGTT+TGG - chr2_2:48215492-48215511 Msa0254650:CDS 30.0%
! TGGGGTCAATTTGAAAATTT+TGG - chr2_2:48215756-48215775 Msa0254650:intron 30.0%
! TTAGGTGACGTAATATTTTG+AGG + chr2_2:48215973-48215992 None:intergenic 30.0%
! CATGTGAACAAAGATTAGTT+GGG + chr2_2:48216085-48216104 None:intergenic 30.0%
AAGTTTATGCAATGAAACCA+AGG - chr2_2:48216492-48216511 Msa0254650:intron 30.0%
AGTTTATGCAATGAAACCAA+GGG - chr2_2:48216493-48216512 Msa0254650:intron 30.0%
GTTAATCCTAAAAAACCAAC+GGG - chr2_2:48216599-48216618 Msa0254650:intron 30.0%
!!! GAATTTTTTGGTGGATTTGT+TGG + chr2_2:48216680-48216699 None:intergenic 30.0%
!!! TCATCATCAGAATTTTTTGG+TGG + chr2_2:48216689-48216708 None:intergenic 30.0%
! TGAGTGGTGATTTTTCTTTT+TGG - chr2_2:48216737-48216756 Msa0254650:CDS 30.0%
!!! TTTTTAAGTAGTCTAGTGGC+TGG - chr2_2:48215466-48215485 Msa0254650:CDS 35.0%
TCTTAAAGTGTGTTTGGATG+AGG - chr2_2:48215498-48215517 Msa0254650:CDS 35.0%
TCCTTCTAAACACACTCTTA+AGG - chr2_2:48216001-48216020 Msa0254650:intron 35.0%
! ACCTTAAGAGTGTGTTTAGA+AGG + chr2_2:48216005-48216024 None:intergenic 35.0%
GTGAATAAGAGAGAAATTCG+AGG - chr2_2:48216023-48216042 Msa0254650:intron 35.0%
! GAACAAAGATTAGTTGGGTA+GGG + chr2_2:48216080-48216099 None:intergenic 35.0%
! TGAACAAAGATTAGTTGGGT+AGG + chr2_2:48216081-48216100 None:intergenic 35.0%
CCATGTGAACAAAGATTAGT+TGG + chr2_2:48216086-48216105 None:intergenic 35.0%
CCAACTAATCTTTGTTCACA+TGG - chr2_2:48216083-48216102 Msa0254650:intron 35.0%
TCATAGTGCATGTGAAAACA+TGG + chr2_2:48216211-48216230 None:intergenic 35.0%
GTTTATGCAATGAAACCAAG+GGG - chr2_2:48216494-48216513 Msa0254650:intron 35.0%
TAACCATCATTTGTCGATGT+TGG + chr2_2:48216551-48216570 None:intergenic 35.0%
GATGAAGATCAACTTGACAA+TGG - chr2_2:48216707-48216726 Msa0254650:intron 35.0%
!!! TTTTTAAGTAGTCTAGTGGC+TGG - chr2_2:48215466-48215485 Msa0254650:CDS 35.0%
TCTTAAAGTGTGTTTGGATG+AGG - chr2_2:48215498-48215517 Msa0254650:CDS 35.0%
TCCTTCTAAACACACTCTTA+AGG - chr2_2:48216001-48216020 Msa0254650:intron 35.0%
! ACCTTAAGAGTGTGTTTAGA+AGG + chr2_2:48216005-48216024 None:intergenic 35.0%
GTGAATAAGAGAGAAATTCG+AGG - chr2_2:48216023-48216042 Msa0254650:intron 35.0%
! GAACAAAGATTAGTTGGGTA+GGG + chr2_2:48216080-48216099 None:intergenic 35.0%
! TGAACAAAGATTAGTTGGGT+AGG + chr2_2:48216081-48216100 None:intergenic 35.0%
CCATGTGAACAAAGATTAGT+TGG + chr2_2:48216086-48216105 None:intergenic 35.0%
CCAACTAATCTTTGTTCACA+TGG - chr2_2:48216083-48216102 Msa0254650:intron 35.0%
TCATAGTGCATGTGAAAACA+TGG + chr2_2:48216211-48216230 None:intergenic 35.0%
GTTTATGCAATGAAACCAAG+GGG - chr2_2:48216494-48216513 Msa0254650:intron 35.0%
TAACCATCATTTGTCGATGT+TGG + chr2_2:48216551-48216570 None:intergenic 35.0%
GATGAAGATCAACTTGACAA+TGG - chr2_2:48216707-48216726 Msa0254650:intron 35.0%
TTAGACGCATATTTGAGGGT+GGG + chr2_2:48215363-48215382 None:intergenic 40.0%
TTTAGACGCATATTTGAGGG+TGG + chr2_2:48215364-48215383 None:intergenic 40.0%
TAGGGACGTTACATTATCCA+GGG + chr2_2:48216062-48216081 None:intergenic 40.0%
TCGCTTATAATAATGACCGG+AGG - chr2_2:48216254-48216273 Msa0254650:intron 40.0%
ATGTACACATTAGGGACTCA+TGG - chr2_2:48216277-48216296 Msa0254650:intron 40.0%
GATCCAACATCGACAAATGA+TGG - chr2_2:48216545-48216564 Msa0254650:intron 40.0%
ATCAGAGGTTTATGCGTTTG+CGG + chr2_2:48216656-48216675 None:intergenic 40.0%
TTAGACGCATATTTGAGGGT+GGG + chr2_2:48215363-48215382 None:intergenic 40.0%
TTTAGACGCATATTTGAGGG+TGG + chr2_2:48215364-48215383 None:intergenic 40.0%
TAGGGACGTTACATTATCCA+GGG + chr2_2:48216062-48216081 None:intergenic 40.0%
TCGCTTATAATAATGACCGG+AGG - chr2_2:48216254-48216273 Msa0254650:intron 40.0%
ATGTACACATTAGGGACTCA+TGG - chr2_2:48216277-48216296 Msa0254650:intron 40.0%
GATCCAACATCGACAAATGA+TGG - chr2_2:48216545-48216564 Msa0254650:intron 40.0%
ATCAGAGGTTTATGCGTTTG+CGG + chr2_2:48216656-48216675 None:intergenic 40.0%
TTTCAAATGGCTCTTGCAGC+AGG - chr2_2:48215312-48215331 Msa0254650:three_prime_UTR 45.0%
GTAGGGACGTTACATTATCC+AGG + chr2_2:48216063-48216082 None:intergenic 45.0%
ACCGGAGGATGTACACATTA+GGG - chr2_2:48216269-48216288 Msa0254650:intron 45.0%
TCCCTAATGTGTACATCCTC+CGG + chr2_2:48216273-48216292 None:intergenic 45.0%
!!! GTGCTACCCGTTGGTTTTTT+AGG + chr2_2:48216608-48216627 None:intergenic 45.0%
AAAAAACCAACGGGTAGCAC+AGG - chr2_2:48216608-48216627 Msa0254650:intron 45.0%
!! GGTGGATTTGTTGGTATCAG+AGG + chr2_2:48216671-48216690 None:intergenic 45.0%
TGACAATGGCAATGAGTGAG+TGG - chr2_2:48216721-48216740 Msa0254650:intron 45.0%
TTTCAAATGGCTCTTGCAGC+AGG - chr2_2:48215312-48215331 Msa0254650:three_prime_UTR 45.0%
GTAGGGACGTTACATTATCC+AGG + chr2_2:48216063-48216082 None:intergenic 45.0%
ACCGGAGGATGTACACATTA+GGG - chr2_2:48216269-48216288 Msa0254650:intron 45.0%
TCCCTAATGTGTACATCCTC+CGG + chr2_2:48216273-48216292 None:intergenic 45.0%
!!! GTGCTACCCGTTGGTTTTTT+AGG + chr2_2:48216608-48216627 None:intergenic 45.0%
AAAAAACCAACGGGTAGCAC+AGG - chr2_2:48216608-48216627 Msa0254650:intron 45.0%
!! GGTGGATTTGTTGGTATCAG+AGG + chr2_2:48216671-48216690 None:intergenic 45.0%
TGACAATGGCAATGAGTGAG+TGG - chr2_2:48216721-48216740 Msa0254650:intron 45.0%
GACCGGAGGATGTACACATT+AGG - chr2_2:48216268-48216287 Msa0254650:intron 50.0%
ATGAAACCAAGGGGTAGCAC+AGG - chr2_2:48216503-48216522 Msa0254650:intron 50.0%
GACCGGAGGATGTACACATT+AGG - chr2_2:48216268-48216287 Msa0254650:intron 50.0%
ATGAAACCAAGGGGTAGCAC+AGG - chr2_2:48216503-48216522 Msa0254650:intron 50.0%
TATTTGAGGGTGGGCAGCAC+TGG + chr2_2:48215354-48215373 None:intergenic 55.0%
GAGGTTCGAACTTCGATCCC+TGG - chr2_2:48216042-48216061 Msa0254650:intron 55.0%
TACCAACTTCGGTCACGACC+AGG - chr2_2:48216180-48216199 Msa0254650:intron 55.0%
TGTGAAAACATGGAGCCGCC+TGG + chr2_2:48216201-48216220 None:intergenic 55.0%
AGCACAGGAAGCGGAGGATT+CGG - chr2_2:48216518-48216537 Msa0254650:intron 55.0%
TAGCACAGGAAGCGGAGGAT+TGG - chr2_2:48216517-48216536 Msa0254650:intron 55.0%
AGCACAGGAAGCGGAGGATT+GGG - chr2_2:48216518-48216537 Msa0254650:intron 55.0%
TATTTGAGGGTGGGCAGCAC+TGG + chr2_2:48215354-48215373 None:intergenic 55.0%
GAGGTTCGAACTTCGATCCC+TGG - chr2_2:48216042-48216061 Msa0254650:intron 55.0%
TACCAACTTCGGTCACGACC+AGG - chr2_2:48216180-48216199 Msa0254650:intron 55.0%
TGTGAAAACATGGAGCCGCC+TGG + chr2_2:48216201-48216220 None:intergenic 55.0%
AGCACAGGAAGCGGAGGATT+CGG - chr2_2:48216518-48216537 Msa0254650:intron 55.0%
TAGCACAGGAAGCGGAGGAT+TGG - chr2_2:48216517-48216536 Msa0254650:intron 55.0%
AGCACAGGAAGCGGAGGATT+GGG - chr2_2:48216518-48216537 Msa0254650:intron 55.0%
CAACTTCGGTCACGACCAGG+CGG - chr2_2:48216183-48216202 Msa0254650:intron 60.0%
CCAAGGGGTAGCACAGGAAG+CGG - chr2_2:48216509-48216528 Msa0254650:intron 60.0%
GCACAGGAAGCGGAGGATTC+GGG - chr2_2:48216519-48216538 Msa0254650:intron 60.0%
AGGAAGCGGAGGATTCGGGA+AGG - chr2_2:48216523-48216542 Msa0254650:intron 60.0%
CCAACGGGTAGCACAGGAAG+CGG - chr2_2:48216614-48216633 Msa0254650:intron 60.0%
GCACAGGAAGCGGAGGATTG+GGG - chr2_2:48216624-48216643 Msa0254650:intron 60.0%
CAACTTCGGTCACGACCAGG+CGG - chr2_2:48216183-48216202 Msa0254650:intron 60.0%
CCAAGGGGTAGCACAGGAAG+CGG - chr2_2:48216509-48216528 Msa0254650:intron 60.0%
GCACAGGAAGCGGAGGATTC+GGG - chr2_2:48216519-48216538 Msa0254650:intron 60.0%
AGGAAGCGGAGGATTCGGGA+AGG - chr2_2:48216523-48216542 Msa0254650:intron 60.0%
CCAACGGGTAGCACAGGAAG+CGG - chr2_2:48216614-48216633 Msa0254650:intron 60.0%
GCACAGGAAGCGGAGGATTG+GGG - chr2_2:48216624-48216643 Msa0254650:intron 60.0%
!! GGTGGGCAGCACTGGCATGT+GGG + chr2_2:48215346-48215365 None:intergenic 65.0%
CGCCTGGTCGTGACCGAAGT+TGG + chr2_2:48216185-48216204 None:intergenic 65.0%
CCGCTTCCTGTGCTACCCCT+TGG + chr2_2:48216512-48216531 None:intergenic 65.0%
AGGGGTAGCACAGGAAGCGG+AGG - chr2_2:48216512-48216531 Msa0254650:intron 65.0%
CCGCTTCCTGTGCTACCCGT+TGG + chr2_2:48216617-48216636 None:intergenic 65.0%
ACGGGTAGCACAGGAAGCGG+AGG - chr2_2:48216617-48216636 Msa0254650:intron 65.0%
!! GGTGGGCAGCACTGGCATGT+GGG + chr2_2:48215346-48215365 None:intergenic 65.0%
CGCCTGGTCGTGACCGAAGT+TGG + chr2_2:48216185-48216204 None:intergenic 65.0%
CCGCTTCCTGTGCTACCCCT+TGG + chr2_2:48216512-48216531 None:intergenic 65.0%
AGGGGTAGCACAGGAAGCGG+AGG - chr2_2:48216512-48216531 Msa0254650:intron 65.0%
CCGCTTCCTGTGCTACCCGT+TGG + chr2_2:48216617-48216636 None:intergenic 65.0%
ACGGGTAGCACAGGAAGCGG+AGG - chr2_2:48216617-48216636 Msa0254650:intron 65.0%
!! GGGTGGGCAGCACTGGCATG+TGG + chr2_2:48215347-48215366 None:intergenic 70.0%
!! GGGTGGGCAGCACTGGCATG+TGG + chr2_2:48215347-48215366 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 48215219 48216919 48215219 ID=Msa0254650;Name=Msa0254650
chr2_2 mRNA 48215219 48216919 48215219 ID=Msa0254650-mRNA-1;Parent=Msa0254650;Name=Msa0254650-mRNA-1;_AED=0.05;_eAED=0.05;_QI=99|1|1|1|1|1|2|180|121
chr2_2 exon 48215219 48215678 48215219 ID=Msa0254650-mRNA-1:exon:14560;Parent=Msa0254650-mRNA-1
chr2_2 exon 48216735 48216919 48216735 ID=Msa0254650-mRNA-1:exon:14559;Parent=Msa0254650-mRNA-1
chr2_2 five_prime_UTR 48216821 48216919 48216821 ID=Msa0254650-mRNA-1:five_prime_utr;Parent=Msa0254650-mRNA-1
chr2_2 CDS 48216735 48216820 48216735 ID=Msa0254650-mRNA-1:cds;Parent=Msa0254650-mRNA-1
chr2_2 CDS 48215399 48215678 48215399 ID=Msa0254650-mRNA-1:cds;Parent=Msa0254650-mRNA-1
chr2_2 three_prime_UTR 48215219 48215398 48215219 ID=Msa0254650-mRNA-1:three_prime_utr;Parent=Msa0254650-mRNA-1
Gene Sequence

>Msa0254650

ATGGCTCTTGCAGCAGGTAGCTTCACCCACATGCCAGTGCTGCCCACCCTCAAATATGCGTCTAAAAAATCTATGACAATTACCATAAAATGTGCTAACAACAACAAAAACAAAACAAAAGTTTATGCAATGAAACCAAGGGGTAGCACAGGAAGCGGAGGATTCGGGAAGGATCCAACATCGACAAATGATGGTTATGATGAACATGACGAAAACAAAATAAAAGTTAATCCTAAAAAACCAACGGGTAGCACAGGAAGCGGAGGATTGGGGACTGAACCGCAAACGCATAAACCTCTGATACCAACAAATCCACCAAAAAATTCTGATGATGATGAAGATCAACTTGACAATGGCAATGAGTGA
Protein sequence

>Msa0254650

MALAAGSFTHMPVLPTLKYASKKSMTITIKCANNNKNKTKVYAMKPRGSTGSGGFGKDPTSTNDGYDEHDENKIKVNPKKPTGSTGSGGLGTEPQTHKPLIPTNPPKNSDDDEDQLDNGNE*