AlfalfaGEDB | Msa0275720

M. sativa cultivar zhongmu-4 / Msa0275720

KEGG: -
GO: -
PFAM: PF00646.36

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0275720	XP_013462780.1	72.973	185	38	2	8	180	11	195	4.86e-69	218

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0275720	A0A072V585	72.973	185	38	2	8	180	11	195	2.32e-69	218

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0043980	Msa0275720	0.816894	4.371368e-52	-8.615850e-47
Msa0151180	Msa0275720	0.815166	1.062588e-51	-8.615850e-47
Msa0196410	Msa0275720	0.817793	2.742179e-52	-8.615850e-47
Msa0206010	Msa0275720	0.814521	1.476903e-51	-8.615850e-47
Msa0238320	Msa0275720	0.834218	3.402536e-56	-8.615850e-47
Msa0275720	Msa0287410	0.805354	1.389836e-49	-8.615850e-47
Msa0275720	Msa0326110	0.825461	4.628703e-54	-8.615850e-47
Msa0275720	Msa0426900	0.801658	8.115399e-49	-8.615850e-47
Msa0275720	Msa0617640	0.804945	1.692620e-49	-8.615850e-47
Msa0275720	Msa0999100	0.813824	2.104343e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0275720	MtrunA17_Chr2g0288061	72.316	177	37	2	8	172	11	187	3.30e-67	203
Msa0275720	MtrunA17_Chr2g0287321	32.222	180	91	3	1	172	11	167	1.56e-17	75.9
Msa0275720	MtrunA17_Chr2g0286581	32.620	187	104	5	1	174	12	189	3.06e-16	73.2
Msa0275720	MtrunA17_Chr2g0287371	54.430	79	28	1	1	71	5	83	1.18e-15	72.0
Msa0275720	MtrunA17_Chr2g0287401	31.319	182	92	2	1	174	2	158	3.80e-15	69.3
Msa0275720	MtrunA17_Chr2g0287311	47.297	74	31	1	1	66	12	85	2.24e-12	62.8
Msa0275720	MtrunA17_Chr2g0287391	34.043	188	97	6	2	174	3	178	7.22e-12	60.8
Msa0275720	MtrunA17_Chr1g0156951	31.330	233	91	9	1	174	1	223	3.37e-11	59.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 28 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGGACCTGCAGAATGTTT+TGG	0.341546	2_3:-7893553	None:intergenic
AAGAGGAGAAAGAATCGGCG+TGG	0.679826	2_3:+7893393	Msa0275720:intron
AAGTAGTTATTGAGTTTCTG+CGG	0.650429	2_3:+7893417	Msa0275720:CDS
AATTGAGGTGCGTATGCAAA+TGG	0.587049	2_3:+7892904	Msa0275720:CDS
ACAGAACGCGATACTCCCGG+AGG	0.809853	2_3:+7892833	Msa0275720:CDS
ACGACGGCGCTGATGGCGGA+AGG	0.604197	2_3:+7892798	None:intergenic
AGATGAAATGCAAATAGCGG+AGG	0.653702	2_3:+7893514	Msa0275720:CDS
ATTTCGATAATCAGATCCTC+CGG	0.496089	2_3:-7892849	None:intergenic
CAAGCGTTTATCTGCAATTG+AGG	0.488368	2_3:+7892889	Msa0275720:CDS
CAGAGATGAAATGCAAATAG+CGG	0.644769	2_3:+7893511	Msa0275720:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGAAGACTTTAATCGTTTAA+CGG	-	chr2_3:7893015-7893034	None:intergenic	30.0%
AAGTAGTTATTGAGTTTCTG+CGG	+	chr2_3:7893417-7893436	Msa0275720:CDS	30.0%
CATTCATGACTTGAGATAAA+CGG	-	chr2_3:7893574-7893593	None:intergenic	30.0%
GGAAGACTTTAATCGTTTAA+CGG	-	chr2_3:7893015-7893034	None:intergenic	30.0%
AAGTAGTTATTGAGTTTCTG+CGG	+	chr2_3:7893417-7893436	Msa0275720:CDS	30.0%
CATTCATGACTTGAGATAAA+CGG	-	chr2_3:7893574-7893593	None:intergenic	30.0%
ATTTCGATAATCAGATCCTC+CGG	-	chr2_3:7892852-7892871	None:intergenic	35.0%
AGAAGAAGAAGAAGATGAAG+AGG	+	chr2_3:7893073-7893092	Msa0275720:CDS	35.0%
TGAAGAAGAGGAGAAAGAAT+CGG	+	chr2_3:7893388-7893407	Msa0275720:intron	35.0%
CAGAGATGAAATGCAAATAG+CGG	+	chr2_3:7893511-7893530	Msa0275720:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	7892810	7893598	7892810	ID=Msa0275720;Name=Msa0275720
chr2_3	mRNA	7892810	7893598	7892810	ID=Msa0275720-mRNA-1;Parent=Msa0275720;Name=Msa0275720-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0\|0\|0\|1\|0\|0\|3\|0\|180
chr2_3	exon	7892810	7892938	7892810	ID=Msa0275720-mRNA-1:exon:3657;Parent=Msa0275720-mRNA-1
chr2_3	exon	7892963	7893175	7892963	ID=Msa0275720-mRNA-1:exon:3658;Parent=Msa0275720-mRNA-1
chr2_3	exon	7893398	7893598	7893398	ID=Msa0275720-mRNA-1:exon:3659;Parent=Msa0275720-mRNA-1
chr2_3	CDS	7892810	7892938	7892810	ID=Msa0275720-mRNA-1:cds;Parent=Msa0275720-mRNA-1
chr2_3	CDS	7892963	7893175	7892963	ID=Msa0275720-mRNA-1:cds;Parent=Msa0275720-mRNA-1
chr2_3	CDS	7893398	7893598	7893398	ID=Msa0275720-mRNA-1:cds;Parent=Msa0275720-mRNA-1

Gene Sequence

>Msa0275720

ATGGCGGAAGGCACGGCGGAGTCACAGAACGCGATACTCCCGGAGGATCTGATTATCGAAATCCTTTCTAGAGTTGAACCAAGCGTTTATCTGCAATTGAGGTGCGTATGCAAATGGTGGAAATCACTTAAGCACATTCGTATATCGATAACTGAAATCATGTATCTAGTTTGTGATGCCGTTAAACGATTAAAGTCTTCCGAGTTGCTTCGAAAAGAAGAGGATTTGGATGGTGAAGAAGAAGAAGAAGAAGATGAAGAGGATTTTGATGATGATGACGAAGAAGATTTTGATGAAGAAGAGGAAGATGACGAGGAAAATTTTGATGAGGAAGAAGAAGAAGAGAAAGAATCGGCGTGGAAAGTAGTTATTGAGTTTCTGCGGCAGAAGGAGGCAGTTCCGTTGGATAATTTTTTGAAGATTTTGAGGGGTATCAAAGAGCAATATGAAAGTATCAGAGATGAAATGCAAATAGCGGAGGATAGAGTGAAGTGTCTCCAAAACATTCTGCAGGTCCGTTTATCTCAAGTCATGAATGAGTGA

Protein sequence

>Msa0275720

MAEGTAESQNAILPEDLIIEILSRVEPSVYLQLRCVCKWWKSLKHIRISITEIMYLVCDAVKRLKSSELLRKEEDLDGEEEEEEDEEDFDDDDEEDFDEEEEDDEENFDEEEEEEKESAWKVVIEFLRQKEAVPLDNFLKILRGIKEQYESIRDEMQIAEDRVKCLQNILQVRLSQVMNE*