AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0238320


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0238320 MtrunA17_Chr2g0288061 71.287 101 21 1 8 108 11 103 3.34e-34 122
Msa0238320 MtrunA17_Chr2g0288061 68.367 98 18 2 161 258 103 187 3.93e-28 106
Msa0238320 MtrunA17_Chr2g0287401 52.703 74 35 0 1 74 2 75 7.74e-16 73.2
Msa0238320 MtrunA17_Chr1g0170131 48.529 68 31 1 1 68 1 64 3.16e-15 72.8
Msa0238320 MtrunA17_Chr2g0286581 61.111 54 21 0 1 54 12 65 6.54e-15 71.2
Msa0238320 MtrunA17_Chr2g0287311 50.000 74 37 0 1 74 12 85 1.07e-14 71.2
Msa0238320 MtrunA17_Chr2g0287421 61.111 54 21 0 1 54 2 55 2.40e-14 69.3
Msa0238320 MtrunA17_Chr2g0287321 52.055 73 35 0 1 73 11 83 6.03e-14 68.2
Msa0238320 MtrunA17_Chr2g0287371 52.344 128 47 1 1 128 5 118 2.45e-13 67.8
Msa0238320 MtrunA17_Chr1g0156951 32.000 275 120 10 1 260 1 223 8.98e-13 65.9
Msa0238320 MtrunA17_Chr2g0287351 40.404 99 48 2 1 90 1 97 9.31e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCGAAAAGAAGAGGATT+TGG 0.329420 2_2:+11765258 Msa0238320:CDS
AACGGACCTGCAGAATGTTT+TGG 0.341546 2_2:-11765783 None:intergenic
GGAAGACTTTAATCGTTTAA+CGG 0.443117 2_2:-11765230 None:intergenic
CATTCATGACTTGAGATAAA+CGG 0.459596 2_2:-11765801 None:intergenic
CGCTTGGTTCAACTCTAGAA+AGG 0.462208 2_2:-11765090 None:intergenic
GGCGCTGATGGCGGAAGCCA+CGG 0.464104 2_2:+11765021 None:intergenic
TGATGAAGAAGAGGAAGATG+AGG 0.469022 2_2:+11765318 Msa0238320:CDS
CGAGTTGCTTCGAAAAGAAG+AGG 0.487698 2_2:+11765252 Msa0238320:CDS
CAAGCGTTTATCTGCAATTG+AGG 0.488368 2_2:+11765107 Msa0238320:CDS
ATTTCGATAATCAGATCCTC+CGG 0.496089 2_2:-11765067 None:intergenic
CTCAATTGCAGATAAACGCT+TGG 0.514934 2_2:-11765106 None:intergenic
GTGTCTCCAAAACATTCTGC+AGG 0.545478 2_2:+11765777 Msa0238320:CDS
TATTGAGTTTCTGCGGCAGA+AGG 0.548094 2_2:+11765654 Msa0238320:CDS
TGAAGAAGAGGAAGATGAGG+AGG 0.549099 2_2:+11765321 Msa0238320:CDS
GCAGAAGGAGGCAGTTCCGT+TGG 0.553372 2_2:+11765669 Msa0238320:CDS
TGAAGAAGAGGAGAAAGAAT+CGG 0.556114 2_2:+11765618 Msa0238320:CDS
GTCACAGAACGCGATACTCC+CGG 0.563110 2_2:+11765048 Msa0238320:CDS
TGGTGGTGGTTCTGAAGAAG+AGG 0.571049 2_2:+11765606 Msa0238320:CDS
CGAAAAGAAGAGGATTTGGA+TGG 0.572187 2_2:+11765262 Msa0238320:CDS
AATTGAGGTGCGTATGCAAA+TGG 0.587049 2_2:+11765122 Msa0238320:CDS
TGAGTTTCTGCGGCAGAAGG+AGG 0.614751 2_2:+11765657 Msa0238320:CDS
CGCGTTCTGTGACTCCGCCG+TGG 0.622877 2_2:-11765038 None:intergenic
TTTCGATAATCAGATCCTCC+GGG 0.622988 2_2:-11765066 None:intergenic
CAGAGATGAAATGCAAATAG+CGG 0.644769 2_2:+11765741 Msa0238320:CDS
GTTGATGAAGACAATGGTGG+TGG 0.645971 2_2:+11765592 Msa0238320:CDS
AAGTAGTTATTGAGTTTCTG+CGG 0.650429 2_2:+11765647 Msa0238320:CDS
AGATGAAATGCAAATAGCGG+AGG 0.653702 2_2:+11765744 Msa0238320:CDS
GAGAATGTTGATGAAGACAA+TGG 0.662623 2_2:+11765586 Msa0238320:CDS
TGAGGTGCGTATGCAAATGG+TGG 0.665483 2_2:+11765125 Msa0238320:CDS
AAGAGGAGAAAGAATCGGCG+TGG 0.679826 2_2:+11765623 Msa0238320:CDS
GCTGATGGCGGAAGCCACGG+CGG 0.707073 2_2:+11765024 None:intergenic
AATGTTGATGAAGACAATGG+TGG 0.715735 2_2:+11765589 Msa0238320:CDS
ACAGAACGCGATACTCCCGG+AGG 0.809853 2_2:+11765051 Msa0238320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTTTGAAGATTTTG+AGG + chr2_2:11765692-11765711 Msa0238320:CDS 15.0%
!!! TAATTTTTTGAAGATTTTGA+GGG + chr2_2:11765693-11765712 Msa0238320:CDS 15.0%
!!! ATAATTTTTTGAAGATTTTG+AGG + chr2_2:11765692-11765711 Msa0238320:CDS 15.0%
!!! TAATTTTTTGAAGATTTTGA+GGG + chr2_2:11765693-11765712 Msa0238320:CDS 15.0%
!!! TGTTTTTGATCGTTATTTAT+TGG + chr2_2:11765156-11765175 Msa0238320:CDS 20.0%
!! ATCTTCAAAAAATTATCCAA+CGG - chr2_2:11765688-11765707 None:intergenic 20.0%
!!! AATTTTTTGAAGATTTTGAG+GGG + chr2_2:11765694-11765713 Msa0238320:CDS 20.0%
!!! TGTTTTTGATCGTTATTTAT+TGG + chr2_2:11765156-11765175 Msa0238320:CDS 20.0%
!! ATCTTCAAAAAATTATCCAA+CGG - chr2_2:11765688-11765707 None:intergenic 20.0%
!!! AATTTTTTGAAGATTTTGAG+GGG + chr2_2:11765694-11765713 Msa0238320:CDS 20.0%
GGAAGACTTTAATCGTTTAA+CGG - chr2_2:11765233-11765252 None:intergenic 30.0%
AAGTAGTTATTGAGTTTCTG+CGG + chr2_2:11765647-11765666 Msa0238320:CDS 30.0%
CATTCATGACTTGAGATAAA+CGG - chr2_2:11765804-11765823 None:intergenic 30.0%
GGAAGACTTTAATCGTTTAA+CGG - chr2_2:11765233-11765252 None:intergenic 30.0%
AAGTAGTTATTGAGTTTCTG+CGG + chr2_2:11765647-11765666 Msa0238320:CDS 30.0%
CATTCATGACTTGAGATAAA+CGG - chr2_2:11765804-11765823 None:intergenic 30.0%
ATTTCGATAATCAGATCCTC+CGG - chr2_2:11765070-11765089 None:intergenic 35.0%
AGAAGAAGAAGAAGATGAAG+AGG + chr2_2:11765291-11765310 Msa0238320:CDS 35.0%
!! AGAGGATTTTGATGAAGAAG+AGG + chr2_2:11765309-11765328 Msa0238320:CDS 35.0%
!! TGAGGAGGAAAATTTTGATG+AGG + chr2_2:11765336-11765355 Msa0238320:CDS 35.0%
! GAGAATGTTGATGAAGACAA+TGG + chr2_2:11765586-11765605 Msa0238320:CDS 35.0%
! AATGTTGATGAAGACAATGG+TGG + chr2_2:11765589-11765608 Msa0238320:CDS 35.0%
TGAAGAAGAGGAGAAAGAAT+CGG + chr2_2:11765618-11765637 Msa0238320:CDS 35.0%
CAGAGATGAAATGCAAATAG+CGG + chr2_2:11765741-11765760 Msa0238320:CDS 35.0%
ATTTCGATAATCAGATCCTC+CGG - chr2_2:11765070-11765089 None:intergenic 35.0%
AGAAGAAGAAGAAGATGAAG+AGG + chr2_2:11765291-11765310 Msa0238320:CDS 35.0%
!! AGAGGATTTTGATGAAGAAG+AGG + chr2_2:11765309-11765328 Msa0238320:CDS 35.0%
!! TGAGGAGGAAAATTTTGATG+AGG + chr2_2:11765336-11765355 Msa0238320:CDS 35.0%
! GAGAATGTTGATGAAGACAA+TGG + chr2_2:11765586-11765605 Msa0238320:CDS 35.0%
! AATGTTGATGAAGACAATGG+TGG + chr2_2:11765589-11765608 Msa0238320:CDS 35.0%
TGAAGAAGAGGAGAAAGAAT+CGG + chr2_2:11765618-11765637 Msa0238320:CDS 35.0%
CAGAGATGAAATGCAAATAG+CGG + chr2_2:11765741-11765760 Msa0238320:CDS 35.0%
TTTCGATAATCAGATCCTCC+GGG - chr2_2:11765069-11765088 None:intergenic 40.0%
CTCAATTGCAGATAAACGCT+TGG - chr2_2:11765109-11765128 None:intergenic 40.0%
CAAGCGTTTATCTGCAATTG+AGG + chr2_2:11765107-11765126 Msa0238320:CDS 40.0%
AATTGAGGTGCGTATGCAAA+TGG + chr2_2:11765122-11765141 Msa0238320:CDS 40.0%
! CTCTTCTTTTCGAAGCAACT+CGG - chr2_2:11765254-11765273 None:intergenic 40.0%
GCTTCGAAAAGAAGAGGATT+TGG + chr2_2:11765258-11765277 Msa0238320:CDS 40.0%
CGAAAAGAAGAGGATTTGGA+TGG + chr2_2:11765262-11765281 Msa0238320:CDS 40.0%
TGATGAAGAAGAGGAAGATG+AGG + chr2_2:11765318-11765337 Msa0238320:CDS 40.0%
AGATGAAATGCAAATAGCGG+AGG + chr2_2:11765744-11765763 Msa0238320:CDS 40.0%
TTTCGATAATCAGATCCTCC+GGG - chr2_2:11765069-11765088 None:intergenic 40.0%
CTCAATTGCAGATAAACGCT+TGG - chr2_2:11765109-11765128 None:intergenic 40.0%
CAAGCGTTTATCTGCAATTG+AGG + chr2_2:11765107-11765126 Msa0238320:CDS 40.0%
AATTGAGGTGCGTATGCAAA+TGG + chr2_2:11765122-11765141 Msa0238320:CDS 40.0%
! CTCTTCTTTTCGAAGCAACT+CGG - chr2_2:11765254-11765273 None:intergenic 40.0%
GCTTCGAAAAGAAGAGGATT+TGG + chr2_2:11765258-11765277 Msa0238320:CDS 40.0%
CGAAAAGAAGAGGATTTGGA+TGG + chr2_2:11765262-11765281 Msa0238320:CDS 40.0%
TGATGAAGAAGAGGAAGATG+AGG + chr2_2:11765318-11765337 Msa0238320:CDS 40.0%
AGATGAAATGCAAATAGCGG+AGG + chr2_2:11765744-11765763 Msa0238320:CDS 40.0%
! CGCTTGGTTCAACTCTAGAA+AGG - chr2_2:11765093-11765112 None:intergenic 45.0%
CGAGTTGCTTCGAAAAGAAG+AGG + chr2_2:11765252-11765271 Msa0238320:CDS 45.0%
TGAAGAAGAGGAAGATGAGG+AGG + chr2_2:11765321-11765340 Msa0238320:CDS 45.0%
! GTTGATGAAGACAATGGTGG+TGG + chr2_2:11765592-11765611 Msa0238320:CDS 45.0%
TATTGAGTTTCTGCGGCAGA+AGG + chr2_2:11765654-11765673 Msa0238320:CDS 45.0%
GTGTCTCCAAAACATTCTGC+AGG + chr2_2:11765777-11765796 Msa0238320:CDS 45.0%
! AACGGACCTGCAGAATGTTT+TGG - chr2_2:11765786-11765805 None:intergenic 45.0%
! CGCTTGGTTCAACTCTAGAA+AGG - chr2_2:11765093-11765112 None:intergenic 45.0%
CGAGTTGCTTCGAAAAGAAG+AGG + chr2_2:11765252-11765271 Msa0238320:CDS 45.0%
TGAAGAAGAGGAAGATGAGG+AGG + chr2_2:11765321-11765340 Msa0238320:CDS 45.0%
! GTTGATGAAGACAATGGTGG+TGG + chr2_2:11765592-11765611 Msa0238320:CDS 45.0%
TATTGAGTTTCTGCGGCAGA+AGG + chr2_2:11765654-11765673 Msa0238320:CDS 45.0%
GTGTCTCCAAAACATTCTGC+AGG + chr2_2:11765777-11765796 Msa0238320:CDS 45.0%
! AACGGACCTGCAGAATGTTT+TGG - chr2_2:11765786-11765805 None:intergenic 45.0%
TGAGGTGCGTATGCAAATGG+TGG + chr2_2:11765125-11765144 Msa0238320:CDS 50.0%
!! TGGTGGTGGTTCTGAAGAAG+AGG + chr2_2:11765606-11765625 Msa0238320:CDS 50.0%
AAGAGGAGAAAGAATCGGCG+TGG + chr2_2:11765623-11765642 Msa0238320:CDS 50.0%
TGAGGTGCGTATGCAAATGG+TGG + chr2_2:11765125-11765144 Msa0238320:CDS 50.0%
!! TGGTGGTGGTTCTGAAGAAG+AGG + chr2_2:11765606-11765625 Msa0238320:CDS 50.0%
AAGAGGAGAAAGAATCGGCG+TGG + chr2_2:11765623-11765642 Msa0238320:CDS 50.0%
GTCACAGAACGCGATACTCC+CGG + chr2_2:11765048-11765067 Msa0238320:CDS 55.0%
TGAGTTTCTGCGGCAGAAGG+AGG + chr2_2:11765657-11765676 Msa0238320:CDS 55.0%
GTCACAGAACGCGATACTCC+CGG + chr2_2:11765048-11765067 Msa0238320:CDS 55.0%
TGAGTTTCTGCGGCAGAAGG+AGG + chr2_2:11765657-11765676 Msa0238320:CDS 55.0%
ACAGAACGCGATACTCCCGG+AGG + chr2_2:11765051-11765070 Msa0238320:CDS 60.0%
GCAGAAGGAGGCAGTTCCGT+TGG + chr2_2:11765669-11765688 Msa0238320:CDS 60.0%
ACAGAACGCGATACTCCCGG+AGG + chr2_2:11765051-11765070 Msa0238320:CDS 60.0%
GCAGAAGGAGGCAGTTCCGT+TGG + chr2_2:11765669-11765688 Msa0238320:CDS 60.0%
CGCGTTCTGTGACTCCGCCG+TGG - chr2_2:11765041-11765060 None:intergenic 70.0%
CGCGTTCTGTGACTCCGCCG+TGG - chr2_2:11765041-11765060 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 11765028 11765828 11765028 ID=Msa0238320;Name=Msa0238320
chr2_2 mRNA 11765028 11765828 11765028 ID=Msa0238320-mRNA-1;Parent=Msa0238320;Name=Msa0238320-mRNA-1;_AED=0.06;_eAED=0.05;_QI=0|-1|0|1|-1|1|1|0|266
chr2_2 exon 11765028 11765828 11765028 ID=Msa0238320-mRNA-1:exon:5032;Parent=Msa0238320-mRNA-1
chr2_2 CDS 11765028 11765828 11765028 ID=Msa0238320-mRNA-1:cds;Parent=Msa0238320-mRNA-1
Gene Sequence

>Msa0238320

ATGGCGGAAGCCACGGCGGAGTCACAGAACGCGATACTCCCGGAGGATCTGATTATCGAAATCCTTTCTAGAGTTGAACCAAGCGTTTATCTGCAATTGAGGTGCGTATGCAAATGGTGGAAATCACTTGTTTTTGATCGTTATTTATTGGAGAAGCACATTCGTATATCGATAACTGAAATCATGTATCTAGTTTGTGATGCCGTTAAACGATTAAAGTCTTCCGAGTTGCTTCGAAAAGAAGAGGATTTGGATGGTGAAGAAGAAGAAGAAGAAGATGAAGAGGATTTTGATGAAGAAGAGGAAGATGAGGAGGAAAATTTTGATGAGGAAGAAGAAGAAGTAGATGATGAAGAAGATTTTGATGAAGAAGAAGATGATGATTATGATGATGAAGATGAAGAAGAAGATTTTGATGAAGATGATGATGAAGAAGAAAACAATGCTGAAGAAGAAGAAGAAGATGATGACGAAGAAGATTTTGATGAAGAAGAAGATGATGATGATGATGAAGATGACGAAGAAGATTTTGATGAAGAAGAAAACAATGCTGAAGAAGAGAATGTTGATGAAGACAATGGTGGTGGTTCTGAAGAAGAGGAGAAAGAATCGGCGTGGAAAGTAGTTATTGAGTTTCTGCGGCAGAAGGAGGCAGTTCCGTTGGATAATTTTTTGAAGATTTTGAGGGGAATCAAAGAGCAATACGAAAGTATCAGAGATGAAATGCAAATAGCGGAGGATAGAGTGAAGTGTCTCCAAAACATTCTGCAGGTCCGTTTATCTCAAGTCATGAATGAGTGA

Protein sequence

>Msa0238320

MAEATAESQNAILPEDLIIEILSRVEPSVYLQLRCVCKWWKSLVFDRYLLEKHIRISITEIMYLVCDAVKRLKSSELLRKEEDLDGEEEEEEDEEDFDEEEEDEEENFDEEEEEVDDEEDFDEEEDDDYDDEDEEEDFDEDDDEEENNAEEEEEDDDEEDFDEEEDDDDDEDDEEDFDEEENNAEEENVDEDNGGGSEEEEKESAWKVVIEFLRQKEAVPLDNFLKILRGIKEQYESIRDEMQIAEDRVKCLQNILQVRLSQVMNE*