AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0289180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0289180 MtrunA17_Chr6g0468581 97.735 309 6 1 18 325 1 309 0.0 622
Msa0289180 MtrunA17_Chr2g0282851 77.350 234 52 1 16 248 49 282 1.82e-135 386
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0289180 AT5G14600.1 73.651 315 76 2 18 325 1 315 4.65e-174 485

Find 80 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTTGTTGTTATGTATT+TGG 0.132518 2_3:-35438659 Msa0289180:CDS
ACATTCATGTGGAAGGTTTC+TGG 0.180697 2_3:-35436484 Msa0289180:three_prime_UTR
TCCTAATTCTTCTTCTGTTA+TGG 0.259735 2_3:-35436655 Msa0289180:CDS
AACAATTAGTAACGGTGATT+TGG 0.271982 2_3:-35438908 Msa0289180:CDS
TCCTGAAGAATTTGCTGGCT+TGG 0.291582 2_3:-35437108 Msa0289180:CDS
CGAAGTTCGAGAAGGGAAAA+TGG 0.297664 2_3:-35436769 Msa0289180:CDS
CCAAACATGAATTTCCTATA+TGG 0.311905 2_3:+35436449 None:intergenic
TTGTTTATGTGAAAGATATA+AGG 0.312566 2_3:-35436818 Msa0289180:intron
GGTTGTGTCGTTCTTGAATC+TGG 0.320717 2_3:-35438625 Msa0289180:CDS
AAAACTAAAGTCCACAATTC+AGG 0.328439 2_3:+35438718 None:intergenic
GATTGGATAGGAAAGTCATT+TGG 0.331486 2_3:-35438793 Msa0289180:CDS
GTCTAAAGTATTCAGCAATA+AGG 0.337791 2_3:-35438770 Msa0289180:CDS
GCCAAGCCAGCAAATTCTTC+AGG 0.357310 2_3:+35437107 None:intergenic
GCCATAACAGAAGAAGAATT+AGG 0.368158 2_3:+35436654 None:intergenic
GGGTTTCCTGAAGAATTTGC+TGG 0.381718 2_3:-35437113 Msa0289180:CDS
ATGTATTTGGAGATTGTTCC+TGG 0.385811 2_3:-35438646 Msa0289180:CDS
AGGGATGACTTTGAGAAGAT+TGG 0.392112 2_3:-35437182 Msa0289180:CDS
AACGGTGATGACGGCTCTAA+TGG 0.392261 2_3:-35436732 Msa0289180:CDS
CTTGAATCTGGTACTGGAAG+TGG 0.400748 2_3:-35438613 Msa0289180:CDS
GGTAATTGTAATCTCCATAT+AGG 0.412202 2_3:-35436463 Msa0289180:three_prime_UTR
GATCCCTCAGATACCGTTAC+TGG 0.416695 2_3:+35438853 None:intergenic
CCATATAGGAAATTCATGTT+TGG 0.417762 2_3:-35436449 Msa0289180:three_prime_UTR
ATTGGATAGGAAAGTCATTT+GGG 0.422230 2_3:-35438792 Msa0289180:CDS
TTTATAATTAAAATATTTGA+GGG 0.453920 2_3:-35436404 Msa0289180:three_prime_UTR
ATGGAAAGCTTAGAGATTAA+CGG 0.454257 2_3:-35436750 Msa0289180:CDS
TCTAAAGTATTCAGCAATAA+GGG 0.459899 2_3:-35438769 Msa0289180:CDS
GTCGTTCTTGAATCTGGTAC+TGG 0.478619 2_3:-35438619 Msa0289180:CDS
TTGCAAGAGGAGGAAACGTT+CGG 0.489818 2_3:-35436700 Msa0289180:CDS
CTTTAACTACTTCACTTGCA+AGG 0.505822 2_3:-35438588 Msa0289180:CDS
ACGGGAAGTACCAGTGAAGT+TGG 0.515604 2_3:+35436947 None:intergenic
GCAAGGGCAGTTGCTCCTAC+AGG 0.518867 2_3:-35438571 Msa0289180:CDS
ACAATGCAATCCAACTTCAC+TGG 0.520816 2_3:-35436957 Msa0289180:intron
TACTTGTTTATTGAAAATGT+TGG 0.523271 2_3:-35438965 Msa0289180:CDS
GAACGTTTCCTCCTCTTGCA+AGG 0.535798 2_3:+35436702 None:intergenic
TTGAATCTGGTACTGGAAGT+GGG 0.536848 2_3:-35438612 Msa0289180:CDS
CGTTGTACTTGTTCAATACA+CGG 0.539892 2_3:+35436990 None:intergenic
ATTTGGCAATTGTTTATGCA+AGG 0.545176 2_3:-35438891 Msa0289180:CDS
ATAAGCAGCTTAGTCACTGC+GGG 0.545723 2_3:-35437158 Msa0289180:CDS
ATTCACAACATTCATGTGGA+AGG 0.547599 2_3:-35436491 Msa0289180:three_prime_UTR
GCGGGAGTGAGGGACATTCA+AGG 0.551907 2_3:-35437140 Msa0289180:CDS
GCTTAGTCACTGCGGGAGTG+AGG 0.551945 2_3:-35437151 Msa0289180:CDS
TTCAAACATTCAGATTGGAT+AGG 0.552647 2_3:-35438805 Msa0289180:CDS
GAAGCCAGTAACGGTATCTG+AGG 0.567229 2_3:-35438857 Msa0289180:CDS
CTTAGTCACTGCGGGAGTGA+GGG 0.567587 2_3:-35437150 Msa0289180:CDS
AAATATTTGAGGGATAACCT+TGG 0.569455 2_3:-35436394 Msa0289180:three_prime_UTR
GGAATAGCTAGCCAAGGTTG+AGG 0.572196 2_3:+35437062 None:intergenic
TAGCACCTACTCCTGAATTG+TGG 0.573848 2_3:-35438729 Msa0289180:CDS
CAACTTTACCGGTTGCGCCA+AGG 0.574577 2_3:+35436377 None:intergenic
ATGGTTCTCTTCCTTGCAAG+AGG 0.575002 2_3:-35436713 Msa0289180:CDS
TTTAACTACTTCACTTGCAA+GGG 0.578833 2_3:-35438587 Msa0289180:CDS
GTTCTCTTCCTTGCAAGAGG+AGG 0.580523 2_3:-35436710 Msa0289180:CDS
GTGAGGGACATTCAAGGTGA+GGG 0.582946 2_3:-35437134 Msa0289180:CDS
GTTGTACTTGTTCAATACAC+GGG 0.584848 2_3:+35436991 None:intergenic
GATTCAAGAACGACACAACC+AGG 0.585429 2_3:+35438628 None:intergenic
AGTAACGGTATCTGAGGGAT+CGG 0.588437 2_3:-35438851 Msa0289180:CDS
TTAGAGATTAACGGTGATGA+CGG 0.592052 2_3:-35436741 Msa0289180:CDS
CTTAATTCACAACATTCATG+TGG 0.594752 2_3:-35436495 Msa0289180:three_prime_UTR
AAAGTCCACAATTCAGGAGT+AGG 0.594923 2_3:+35438724 None:intergenic
TTTAATAGAACAATTAGTAA+CGG 0.596180 2_3:-35438916 Msa0289180:CDS
AACGTATAAACATGTCCTGT+AGG 0.611682 2_3:+35438556 None:intergenic
CGGACATACGAAGTTCGAGA+AGG 0.611846 2_3:-35436777 Msa0289180:CDS
CTCGAACTTCGTATGTCCGT+AGG 0.612530 2_3:+35436781 None:intergenic
TGACCTCTAGCTTCACCACA+TGG 0.618356 2_3:+35436627 None:intergenic
GGACATACGAAGTTCGAGAA+GGG 0.625372 2_3:-35436776 Msa0289180:CDS
TGAGGGACATTCAAGGTGAG+GGG 0.627956 2_3:-35437133 Msa0289180:CDS
ACGTTCGGATGGAGCTAGTG+TGG 0.628192 2_3:-35436685 Msa0289180:CDS
TCTGTTATGGCTAGACCATG+TGG 0.628693 2_3:-35436642 Msa0289180:CDS
AACAGAAGAAGAATTAGGAT+AGG 0.630818 2_3:+35436659 None:intergenic
AACAAAGATCTGGATCAGCT+AGG 0.636973 2_3:-35438522 Msa0289180:intron
AGACCATGTGGTGAAGCTAG+AGG 0.638065 2_3:-35436630 Msa0289180:CDS
AGTGAGGGACATTCAAGGTG+AGG 0.642071 2_3:-35437135 Msa0289180:CDS
GCTGAAGGAATAGCTAGCCA+AGG 0.647664 2_3:+35437056 None:intergenic
CATATAGAATCTGTGTCCTG+TGG 0.649958 2_3:+35438692 None:intergenic
AAGCCAGTAACGGTATCTGA+GGG 0.657172 2_3:-35438856 Msa0289180:CDS
AAGAGGAGGAAACGTTCGGA+TGG 0.658556 2_3:-35436696 Msa0289180:CDS
TGACAACATGAAGCCAGTAA+CGG 0.669534 2_3:-35438866 Msa0289180:CDS
GGTGAAGCTAGAGGTCACAC+TGG 0.683965 2_3:-35436621 Msa0289180:CDS
AAAGTATTCAGCAATAAGGG+CGG 0.684692 2_3:-35438766 Msa0289180:CDS
CATAAGCAGCTTAGTCACTG+CGG 0.689323 2_3:-35437159 Msa0289180:CDS
TTGTACTTGTTCAATACACG+GGG 0.704021 2_3:+35436992 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAAAACAAACAAAAAT+AGG + chr2_3:35438973-35438992 None:intergenic 10.0%
!! TTTAATAGAACAATTAGTAA+CGG - chr2_3:35436473-35436492 Msa0289180:three_prime_UTR 15.0%
!!! TAGCTTTATATATTGTATTA+CGG + chr2_3:35437838-35437857 None:intergenic 15.0%
!! AGTCAATTATATATAAAACA+AGG + chr2_3:35437901-35437920 None:intergenic 15.0%
!! ACATATAAAAGAAGAAAAAA+CGG + chr2_3:35438464-35438483 None:intergenic 15.0%
!! TACTTGTTTATTGAAAATGT+TGG - chr2_3:35436424-35436443 Msa0289180:three_prime_UTR 20.0%
!!! TTTCACCAAAAATATGTTTT+AGG + chr2_3:35437158-35437177 None:intergenic 20.0%
!!! TTTTTACAATGGAAAAACTT+AGG - chr2_3:35437785-35437804 Msa0289180:intron 20.0%
!! ATAATATTACTTGCAATTAC+TGG + chr2_3:35438051-35438070 None:intergenic 20.0%
!! GTAATTGCAAGTAATATTAT+TGG - chr2_3:35438051-35438070 Msa0289180:intron 20.0%
!!! AATATTGTTTTTCTTCTTGT+AGG - chr2_3:35438187-35438206 Msa0289180:intron 20.0%
!!! ATATTGTTTTTCTTCTTGTA+GGG - chr2_3:35438188-35438207 Msa0289180:intron 20.0%
!! CATATAAAAGAAGAAAAAAC+GGG + chr2_3:35438463-35438482 None:intergenic 20.0%
!! TTGTTTATGTGAAAGATATA+AGG - chr2_3:35438571-35438590 Msa0289180:CDS 20.0%
!! TAAAAATAAACTGTTGTATG+AGG + chr2_3:35438873-35438892 None:intergenic 20.0%
!! TTCATTGTTTTCTCTGTTTT+CGG - chr2_3:35436393-35436412 Msa0289180:three_prime_UTR 25.0%
!! TCTAAAGTATTCAGCAATAA+GGG - chr2_3:35436620-35436639 Msa0289180:CDS 25.0%
!! TTTCTAGTGTTGTCAAATAT+TGG - chr2_3:35436953-35436972 Msa0289180:intron 25.0%
! CTACCAAAAAACTAGAATAA+CGG + chr2_3:35437041-35437060 None:intergenic 25.0%
!!! AAACATATTTTTGGTGAAAC+TGG - chr2_3:35437159-35437178 Msa0289180:CDS 25.0%
!!! AACATATTTTTGGTGAAACT+GGG - chr2_3:35437160-35437179 Msa0289180:CDS 25.0%
!! TTGTCAAATTGATTCAATAG+CGG - chr2_3:35437568-35437587 Msa0289180:intron 25.0%
! CATACAAATAAAAAAGGGAA+TGG + chr2_3:35437871-35437890 None:intergenic 25.0%
!!! TCCCTTTTTTATTTGTATGA+AGG - chr2_3:35437872-35437891 Msa0289180:intron 25.0%
! CCCTTCATACAAATAAAAAA+GGG + chr2_3:35437876-35437895 None:intergenic 25.0%
!!! CCCTTTTTTATTTGTATGAA+GGG - chr2_3:35437873-35437892 Msa0289180:intron 25.0%
! ACCCTTCATACAAATAAAAA+AGG + chr2_3:35437877-35437896 None:intergenic 25.0%
! TTATTTAACTGTACTCAATG+CGG - chr2_3:35438120-35438139 Msa0289180:intron 25.0%
! ACATAAACAAACGTATACAA+AGG + chr2_3:35438561-35438580 None:intergenic 25.0%
! TCATTGTTTTCTCTGTTTTC+GGG - chr2_3:35436394-35436413 Msa0289180:three_prime_UTR 30.0%
AACAATTAGTAACGGTGATT+TGG - chr2_3:35436481-35436500 Msa0289180:three_prime_UTR 30.0%
ATTTGGCAATTGTTTATGCA+AGG - chr2_3:35436498-35436517 Msa0289180:three_prime_UTR 30.0%
! GTGTTTTCAAACATTCAGAT+TGG - chr2_3:35436579-35436598 Msa0289180:exon 30.0%
TTCAAACATTCAGATTGGAT+AGG - chr2_3:35436584-35436603 Msa0289180:exon 30.0%
ATTGGATAGGAAAGTCATTT+GGG - chr2_3:35436597-35436616 Msa0289180:CDS 30.0%
! GTCTAAAGTATTCAGCAATA+AGG - chr2_3:35436619-35436638 Msa0289180:CDS 30.0%
AAAACTAAAGTCCACAATTC+AGG + chr2_3:35436674-35436693 None:intergenic 30.0%
!! CAGCTTTGTTGTTATGTATT+TGG - chr2_3:35436730-35436749 Msa0289180:CDS 30.0%
TTTAACTACTTCACTTGCAA+GGG - chr2_3:35436802-35436821 Msa0289180:CDS 30.0%
TGATACATGTGCTTATCTTT+AGG - chr2_3:35436912-35436931 Msa0289180:intron 30.0%
!!! TTGCCGTTATTCTAGTTTTT+TGG - chr2_3:35437035-35437054 Msa0289180:CDS 30.0%
! AGCAGAAAAAAGAGACTTTT+AGG - chr2_3:35437077-35437096 Msa0289180:CDS 30.0%
!! AAATATGTTTTAGGCGGTAA+TGG + chr2_3:35437149-35437168 None:intergenic 30.0%
! TACCGCCTAAAACATATTTT+TGG - chr2_3:35437150-35437169 Msa0289180:CDS 30.0%
!! CACCAAAAATATGTTTTAGG+CGG + chr2_3:35437155-35437174 None:intergenic 30.0%
GTTATCTTCCTTGAAATTTC+GGG + chr2_3:35437206-35437225 None:intergenic 30.0%
GGTTATCTTCCTTGAAATTT+CGG + chr2_3:35437207-35437226 None:intergenic 30.0%
!! ATCTTGCAATTCATTGATTC+AGG + chr2_3:35437228-35437247 None:intergenic 30.0%
AAAATCACGATCAAACTCAT+GGG + chr2_3:35437352-35437371 None:intergenic 30.0%
TTGTAAAAATAAGCCACGTT+GGG + chr2_3:35437774-35437793 None:intergenic 30.0%
ATTGTAAAAATAAGCCACGT+TGG + chr2_3:35437775-35437794 None:intergenic 30.0%
!!! AACGTGGCTTATTTTTACAA+TGG - chr2_3:35437774-35437793 Msa0289180:intron 30.0%
TATTGTATTACGGTGAATAC+AGG + chr2_3:35437828-35437847 None:intergenic 30.0%
ACAAATAAAAAAGGGAATGG+AGG + chr2_3:35437868-35437887 None:intergenic 30.0%
GACTTATTTCAACTGTTATG+CGG - chr2_3:35437917-35437936 Msa0289180:intron 30.0%
TGAAATAGAAGATAGTAACG+CGG + chr2_3:35437957-35437976 None:intergenic 30.0%
TTTCCGTAATATGCTATTGA+TGG - chr2_3:35437986-35438005 Msa0289180:intron 30.0%
! GAAAGATATAAGGACTTTTG+AGG - chr2_3:35438581-35438600 Msa0289180:CDS 30.0%
ATGGAAAGCTTAGAGATTAA+CGG - chr2_3:35438639-35438658 Msa0289180:CDS 30.0%
AACAGAAGAAGAATTAGGAT+AGG + chr2_3:35438733-35438752 None:intergenic 30.0%
TCCTAATTCTTCTTCTGTTA+TGG - chr2_3:35438734-35438753 Msa0289180:CDS 30.0%
CTTAATTCACAACATTCATG+TGG - chr2_3:35438894-35438913 Msa0289180:CDS 30.0%
GGTAATTGTAATCTCCATAT+AGG - chr2_3:35438926-35438945 Msa0289180:CDS 30.0%
CCAAACATGAATTTCCTATA+TGG + chr2_3:35438943-35438962 None:intergenic 30.0%
CCATATAGGAAATTCATGTT+TGG - chr2_3:35438940-35438959 Msa0289180:CDS 30.0%
AAATATTTGAGGGATAACCT+TGG - chr2_3:35438995-35439014 Msa0289180:CDS 30.0%
! TCGGTTTTACAGAATCGATT+CGG - chr2_3:35436557-35436576 Msa0289180:three_prime_UTR 35.0%
GATTGGATAGGAAAGTCATT+TGG - chr2_3:35436596-35436615 Msa0289180:CDS 35.0%
! AAAGTATTCAGCAATAAGGG+CGG - chr2_3:35436623-35436642 Msa0289180:CDS 35.0%
ATGTATTTGGAGATTGTTCC+TGG - chr2_3:35436743-35436762 Msa0289180:CDS 35.0%
CTTTAACTACTTCACTTGCA+AGG - chr2_3:35436801-35436820 Msa0289180:CDS 35.0%
AACGTATAAACATGTCCTGT+AGG + chr2_3:35436836-35436855 None:intergenic 35.0%
! GATTTTCACGAACAAAGATC+TGG - chr2_3:35436857-35436876 Msa0289180:intron 35.0%
!!! TGGTGTTGCAGTTTTTTTAG+CGG + chr2_3:35437007-35437026 None:intergenic 35.0%
! GAATAACGGCAAAAGTATGA+TGG + chr2_3:35437027-35437046 None:intergenic 35.0%
CAAAATCACGATCAAACTCA+TGG + chr2_3:35437353-35437372 None:intergenic 35.0%
!!! GATCGTGATTTTGACTTCTT+TGG - chr2_3:35437360-35437379 Msa0289180:intron 35.0%
ATAAACATGCCCTTCACAAA+TGG + chr2_3:35437432-35437451 None:intergenic 35.0%
ACACATGTATCAAACATCAG+AGG + chr2_3:35437509-35437528 None:intergenic 35.0%
CAATGGAAAAACTTAGGAGT+CGG - chr2_3:35437791-35437810 Msa0289180:intron 35.0%
GAAATAGAAGATAGTAACGC+GGG + chr2_3:35437956-35437975 None:intergenic 35.0%
CTATCTTCTATTTCACGTAG+CGG - chr2_3:35437962-35437981 Msa0289180:intron 35.0%
GTGCCATCAATAGCATATTA+CGG + chr2_3:35437992-35438011 None:intergenic 35.0%
ACTTGAGATGTTCAGTGTAA+AGG - chr2_3:35438149-35438168 Msa0289180:intron 35.0%
GCAAAAATGTTACGACATGA+CGG - chr2_3:35438357-35438376 Msa0289180:intron 35.0%
TTGTACTTGTTCAATACACG+GGG + chr2_3:35438400-35438419 None:intergenic 35.0%
GTTGTACTTGTTCAATACAC+GGG + chr2_3:35438401-35438420 None:intergenic 35.0%
CGTTGTACTTGTTCAATACA+CGG + chr2_3:35438402-35438421 None:intergenic 35.0%
CGTAGTAGTTATGCAACTAA+TGG - chr2_3:35438508-35438527 Msa0289180:intron 35.0%
TTAGAGATTAACGGTGATGA+CGG - chr2_3:35438648-35438667 Msa0289180:CDS 35.0%
GCCATAACAGAAGAAGAATT+AGG + chr2_3:35438738-35438757 None:intergenic 35.0%
ATTCACAACATTCATGTGGA+AGG - chr2_3:35438898-35438917 Msa0289180:CDS 35.0%
TGACAACATGAAGCCAGTAA+CGG - chr2_3:35436523-35436542 Msa0289180:three_prime_UTR 40.0%
AAAGTCCACAATTCAGGAGT+AGG + chr2_3:35436668-35436687 None:intergenic 40.0%
!!! GGACTTTAGTTTTAAGCCAC+AGG - chr2_3:35436681-35436700 Msa0289180:CDS 40.0%
CATATAGAATCTGTGTCCTG+TGG + chr2_3:35436700-35436719 None:intergenic 40.0%
! TTGAATCTGGTACTGGAAGT+GGG - chr2_3:35436777-35436796 Msa0289180:CDS 40.0%
AACAAAGATCTGGATCAGCT+AGG - chr2_3:35436867-35436886 Msa0289180:intron 40.0%
ATATTGGCGCTATAGCATAG+CGG - chr2_3:35436969-35436988 Msa0289180:CDS 40.0%
TTAGGCAGTGTTGTCAATTG+TGG - chr2_3:35437095-35437114 Msa0289180:CDS 40.0%
GTATGGACGGAATCAGAATT+TGG + chr2_3:35437295-35437314 None:intergenic 40.0%
!!! GATTTTGACTTCTTTGGCCA+TGG - chr2_3:35437366-35437385 Msa0289180:intron 40.0%
GATTTCCATGAGCATATTGC+TGG - chr2_3:35437458-35437477 Msa0289180:intron 40.0%
ATCAAACATCAGAGGAAGTC+TGG + chr2_3:35437501-35437520 None:intergenic 40.0%
ATTATTGTGCTATCGCATGG+TGG - chr2_3:35437592-35437611 Msa0289180:intron 40.0%
! TGCTATTGATGGCACTTTCA+TGG - chr2_3:35437997-35438016 Msa0289180:intron 40.0%
! AGGGATGACTTTGAGAAGAT+TGG - chr2_3:35438207-35438226 Msa0289180:intron 40.0%
!!! CTTGGCTGATGCAGTTTTTT+TGG - chr2_3:35438299-35438318 Msa0289180:intron 40.0%
! TTTTGGATCTACCTCAACCT+TGG - chr2_3:35438316-35438335 Msa0289180:intron 40.0%
! CGTAACATTTTTGCAGCTGA+AGG + chr2_3:35438351-35438370 None:intergenic 40.0%
ACAATGCAATCCAACTTCAC+TGG - chr2_3:35438432-35438451 Msa0289180:intron 40.0%
ACATTCATGTGGAAGGTTTC+TGG - chr2_3:35438905-35438924 Msa0289180:CDS 40.0%
!!! TTTCTCTGTTTTCGGGTTGC+AGG - chr2_3:35436401-35436420 Msa0289180:three_prime_UTR 45.0%
AAGCCAGTAACGGTATCTGA+GGG - chr2_3:35436533-35436552 Msa0289180:three_prime_UTR 45.0%
AGTAACGGTATCTGAGGGAT+CGG - chr2_3:35436538-35436557 Msa0289180:three_prime_UTR 45.0%
TAGCACCTACTCCTGAATTG+TGG - chr2_3:35436660-35436679 Msa0289180:CDS 45.0%
GATTCAAGAACGACACAACC+AGG + chr2_3:35436764-35436783 None:intergenic 45.0%
! GGTTGTGTCGTTCTTGAATC+TGG - chr2_3:35436764-35436783 Msa0289180:CDS 45.0%
GTCGTTCTTGAATCTGGTAC+TGG - chr2_3:35436770-35436789 Msa0289180:CDS 45.0%
! CTTGAATCTGGTACTGGAAG+TGG - chr2_3:35436776-35436795 Msa0289180:CDS 45.0%
GCAGTGTTGTCAATTGTGGA+AGG - chr2_3:35437099-35437118 Msa0289180:CDS 45.0%
!! TTTTTGGTGAAACTGGGTCC+TGG - chr2_3:35437166-35437185 Msa0289180:CDS 45.0%
!! TCCTTGAAATTTCGGGTTCG+CGG + chr2_3:35437199-35437218 None:intergenic 45.0%
ACCGCAGAGAATTGTAGGTA+CGG + chr2_3:35437255-35437274 None:intergenic 45.0%
! GAATCAGAATTTGGTGGGTG+CGG + chr2_3:35437286-35437305 None:intergenic 45.0%
GGACGGAATCAGAATTTGGT+GGG + chr2_3:35437291-35437310 None:intergenic 45.0%
TGGACGGAATCAGAATTTGG+TGG + chr2_3:35437292-35437311 None:intergenic 45.0%
! CGTATGATTTTGCTACCCCT+TGG + chr2_3:35437327-35437346 None:intergenic 45.0%
! TTTGACTTCTTTGGCCATGG+CGG - chr2_3:35437369-35437388 Msa0289180:intron 45.0%
!!! AGATTTTTTTACGCCCGCCA+TGG + chr2_3:35437386-35437405 None:intergenic 45.0%
CTCATCCAGCAATATGCTCA+TGG + chr2_3:35437466-35437485 None:intergenic 45.0%
GGCATTATTGTGCTATCGCA+TGG - chr2_3:35437589-35437608 Msa0289180:intron 45.0%
ATGATCCCCTATGAAAACCG+CGG - chr2_3:35437621-35437640 Msa0289180:intron 45.0%
CTATCCCAGTTTCGCTATCA+GGG - chr2_3:35437646-35437665 Msa0289180:intron 45.0%
! ATTGCCCTGATAGCGAAACT+GGG + chr2_3:35437653-35437672 None:intergenic 45.0%
! TATTGCCCTGATAGCGAAAC+TGG + chr2_3:35437654-35437673 None:intergenic 45.0%
TTTCGCTATCAGGGCAATAG+CGG - chr2_3:35437655-35437674 Msa0289180:intron 45.0%
CGTTGACATTCTGAGCAGTA+GGG + chr2_3:35438087-35438106 None:intergenic 45.0%
CATAAGCAGCTTAGTCACTG+CGG - chr2_3:35438230-35438249 Msa0289180:intron 45.0%
ATAAGCAGCTTAGTCACTGC+GGG - chr2_3:35438231-35438250 Msa0289180:intron 45.0%
GGGTTTCCTGAAGAATTTGC+TGG - chr2_3:35438276-35438295 Msa0289180:intron 45.0%
!! TCCTGAAGAATTTGCTGGCT+TGG - chr2_3:35438281-35438300 Msa0289180:intron 45.0%
!!! GGACTTTTGAGGTACTCCTA+CGG - chr2_3:35438592-35438611 Msa0289180:CDS 45.0%
GGACATACGAAGTTCGAGAA+GGG - chr2_3:35438613-35438632 Msa0289180:CDS 45.0%
CGAAGTTCGAGAAGGGAAAA+TGG - chr2_3:35438620-35438639 Msa0289180:CDS 45.0%
ATGGTTCTCTTCCTTGCAAG+AGG - chr2_3:35438676-35438695 Msa0289180:CDS 45.0%
TTGCAAGAGGAGGAAACGTT+CGG - chr2_3:35438689-35438708 Msa0289180:CDS 45.0%
TCTGTTATGGCTAGACCATG+TGG - chr2_3:35438747-35438766 Msa0289180:CDS 45.0%
!!! TTTTATAATTAAAATATTTG+AGG - chr2_3:35438984-35439003 Msa0289180:CDS 5.0%
!! TTTATAATTAAAATATTTGA+GGG - chr2_3:35438985-35439004 Msa0289180:CDS 5.0%
GAAGCCAGTAACGGTATCTG+AGG - chr2_3:35436532-35436551 Msa0289180:three_prime_UTR 50.0%
GATCCCTCAGATACCGTTAC+TGG + chr2_3:35436539-35436558 None:intergenic 50.0%
!! AAATTTCGGGTTCGCGGGTT+GGG + chr2_3:35437193-35437212 None:intergenic 50.0%
!! CCTTGAAATTTCGGGTTCGC+GGG + chr2_3:35437198-35437217 None:intergenic 50.0%
GCAGAGAATTGTAGGTACGG+CGG + chr2_3:35437252-35437271 None:intergenic 50.0%
GCCGTACCTACAATTCTCTG+CGG - chr2_3:35437251-35437270 Msa0289180:intron 50.0%
GGATCACCGCAGAGAATTGT+AGG + chr2_3:35437260-35437279 None:intergenic 50.0%
ACAATTCTCTGCGGTGATCC+TGG - chr2_3:35437260-35437279 Msa0289180:intron 50.0%
TTGGCATGCTGGAGTATGGA+CGG + chr2_3:35437308-35437327 None:intergenic 50.0%
TCCATACTCCAGCATGCCAA+GGG - chr2_3:35437308-35437327 Msa0289180:intron 50.0%
TTGACTTCTTTGGCCATGGC+GGG - chr2_3:35437370-35437389 Msa0289180:intron 50.0%
TTCACAAATGGTTGCCACGG+TGG + chr2_3:35437420-35437439 None:intergenic 50.0%
ACCGTGGCAACCATTTGTGA+AGG - chr2_3:35437419-35437438 Msa0289180:intron 50.0%
CCCTTCACAAATGGTTGCCA+CGG + chr2_3:35437423-35437442 None:intergenic 50.0%
CCGTGGCAACCATTTGTGAA+GGG - chr2_3:35437420-35437439 Msa0289180:intron 50.0%
! ATAGCGCCGCGGTTTTCATA+GGG + chr2_3:35437630-35437649 None:intergenic 50.0%
GCTATCCCAGTTTCGCTATC+AGG - chr2_3:35437645-35437664 Msa0289180:intron 50.0%
ATCTGCATATGTGCCCAACG+TGG - chr2_3:35437758-35437777 Msa0289180:intron 50.0%
! GCGTTGACATTCTGAGCAGT+AGG + chr2_3:35438088-35438107 None:intergenic 50.0%
AGTGAGGGACATTCAAGGTG+AGG - chr2_3:35438254-35438273 Msa0289180:intron 50.0%
GTGAGGGACATTCAAGGTGA+GGG - chr2_3:35438255-35438274 Msa0289180:intron 50.0%
TGAGGGACATTCAAGGTGAG+GGG - chr2_3:35438256-35438275 Msa0289180:intron 50.0%
GCCAAGCCAGCAAATTCTTC+AGG + chr2_3:35438285-35438304 None:intergenic 50.0%
GGAATAGCTAGCCAAGGTTG+AGG + chr2_3:35438330-35438349 None:intergenic 50.0%
GCTGAAGGAATAGCTAGCCA+AGG + chr2_3:35438336-35438355 None:intergenic 50.0%
!! ACGGGAAGTACCAGTGAAGT+TGG + chr2_3:35438445-35438464 None:intergenic 50.0%
CTCGAACTTCGTATGTCCGT+AGG + chr2_3:35438611-35438630 None:intergenic 50.0%
CGGACATACGAAGTTCGAGA+AGG - chr2_3:35438612-35438631 Msa0289180:CDS 50.0%
!! AACGGTGATGACGGCTCTAA+TGG - chr2_3:35438657-35438676 Msa0289180:CDS 50.0%
GTTCTCTTCCTTGCAAGAGG+AGG - chr2_3:35438679-35438698 Msa0289180:CDS 50.0%
GAACGTTTCCTCCTCTTGCA+AGG + chr2_3:35438690-35438709 None:intergenic 50.0%
AAGAGGAGGAAACGTTCGGA+TGG - chr2_3:35438693-35438712 Msa0289180:CDS 50.0%
AGACCATGTGGTGAAGCTAG+AGG - chr2_3:35438759-35438778 Msa0289180:CDS 50.0%
! TGACCTCTAGCTTCACCACA+TGG + chr2_3:35438765-35438784 None:intergenic 50.0%
!! GAAATTTCGGGTTCGCGGGT+TGG + chr2_3:35437194-35437213 None:intergenic 55.0%
CCCGCGAACCCGAAATTTCA+AGG - chr2_3:35437195-35437214 Msa0289180:intron 55.0%
GTCCATACTCCAGCATGCCA+AGG - chr2_3:35437307-35437326 Msa0289180:intron 55.0%
CCATACTCCAGCATGCCAAG+GGG - chr2_3:35437309-35437328 Msa0289180:intron 55.0%
TTTGCTACCCCTTGGCATGC+TGG + chr2_3:35437319-35437338 None:intergenic 55.0%
TCTGCCTCACAATTCCACCG+TGG - chr2_3:35437403-35437422 Msa0289180:intron 55.0%
GTTGCCACGGTGGAATTGTG+AGG + chr2_3:35437410-35437429 None:intergenic 55.0%
! TAGCGCCGCGGTTTTCATAG+GGG + chr2_3:35437629-35437648 None:intergenic 55.0%
! GATAGCGCCGCGGTTTTCAT+AGG + chr2_3:35437631-35437650 None:intergenic 55.0%
CTTAGTCACTGCGGGAGTGA+GGG - chr2_3:35438239-35438258 Msa0289180:intron 55.0%
ACGTTCGGATGGAGCTAGTG+TGG - chr2_3:35438704-35438723 Msa0289180:CDS 55.0%
GGTGAAGCTAGAGGTCACAC+TGG - chr2_3:35438768-35438787 Msa0289180:CDS 55.0%
GCAAGGGCAGTTGCTCCTAC+AGG - chr2_3:35436818-35436837 Msa0289180:intron 60.0%
! AGAATTTGGTGGGTGCGGCC+AGG + chr2_3:35437281-35437300 None:intergenic 60.0%
CCCCTTGGCATGCTGGAGTA+TGG + chr2_3:35437312-35437331 None:intergenic 60.0%
GCTTAGTCACTGCGGGAGTG+AGG - chr2_3:35438238-35438257 Msa0289180:intron 60.0%
GCGGGAGTGAGGGACATTCA+AGG - chr2_3:35438249-35438268 Msa0289180:intron 60.0%
GCGAAACTGGGATAGCGCCG+CGG + chr2_3:35437641-35437660 None:intergenic 65.0%
!! CGGGTTCGCGGGTTGGGTCC+AGG + chr2_3:35437187-35437206 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 35436389 35439022 35436389 ID=Msa0289180;Name=Msa0289180
chr2_3 mRNA 35436389 35439022 35436389 ID=Msa0289180-mRNA-1;Parent=Msa0289180;Name=Msa0289180-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|1|0.66|1|1|1|3|204|325
chr2_3 exon 35436389 35436825 35436389 ID=Msa0289180-mRNA-1:exon:11460;Parent=Msa0289180-mRNA-1
chr2_3 exon 35436958 35437202 35436958 ID=Msa0289180-mRNA-1:exon:11459;Parent=Msa0289180-mRNA-1
chr2_3 exon 35438523 35439022 35438523 ID=Msa0289180-mRNA-1:exon:11458;Parent=Msa0289180-mRNA-1
chr2_3 CDS 35438523 35439022 35438523 ID=Msa0289180-mRNA-1:cds;Parent=Msa0289180-mRNA-1
chr2_3 CDS 35436958 35437202 35436958 ID=Msa0289180-mRNA-1:cds;Parent=Msa0289180-mRNA-1
chr2_3 CDS 35436593 35436825 35436593 ID=Msa0289180-mRNA-1:cds;Parent=Msa0289180-mRNA-1
chr2_3 three_prime_UTR 35436389 35436592 35436389 ID=Msa0289180-mRNA-1:three_prime_utr;Parent=Msa0289180-mRNA-1
Gene Sequence

>Msa0289180

ATGTTTCATTGTTTTCTCTGTTTTCGGGTTGCAGGTACTTGTTTATTGAAAATGTTGGCTATCGATTCTACAAGAAAGATGTCATTTAATAGAACAATTAGTAACGGTGATTTGGCAATTGTTTATGCAAGGCATGACAACATGAAGCCAGTAACGGTATCTGAGGGATCGGTTTTACAGAATCGATTCGGTGTTTTCAAACATTCAGATTGGATAGGAAAGTCATTTGGGTCTAAAGTATTCAGCAATAAGGGCGGTTTTGTTTACTTATTAGCACCTACTCCTGAATTGTGGACTTTAGTTTTAAGCCACAGGACACAGATTCTATATGTTGCAGATATCAGCTTTGTTGTTATGTATTTGGAGATTGTTCCTGGTTGTGTCGTTCTTGAATCTGGTACTGGAAGTGGGTCTTTAACTACTTCACTTGCAAGGGCAGTTGCTCCTACAGGACATGTTTATACGTTTGATTTTCACGAACAAAGATCTGGATCAGCTAGGGATGACTTTGAGAAGATTGGCATAAGCAGCTTAGTCACTGCGGGAGTGAGGGACATTCAAGGTGAGGGGTTTCCTGAAGAATTTGCTGGCTTGGCTGATGCAGTTTTTTTGGATCTACCTCAACCTTGGCTAGCTATTCCTTCAGCTGCAAAAATGTTACGACATGACGGCACTTTATGTTCTTTCTCCCCGTGTATTGAACAAGTACAACGATCATGTGAAACAATGCAATCCAACTTCACTGATATAAGGACTTTTGAGGTACTCCTACGGACATACGAAGTTCGAGAAGGGAAAATGGAAAGCTTAGAGATTAACGGTGATGACGGCTCTAATGGTTCTCTTCCTTGCAAGAGGAGGAAACGTTCGGATGGAGCTAGTGTGGTTACCTATCCTAATTCTTCTTCTGTTATGGCTAGACCATGTGGTGAAGCTAGAGGTCACACTGGTTATTTGACATTTGCAAGACTTAATTAA
Protein sequence

>Msa0289180

MFHCFLCFRVAGTCLLKMLAIDSTRKMSFNRTISNGDLAIVYARHDNMKPVTVSEGSVLQNRFGVFKHSDWIGKSFGSKVFSNKGGFVYLLAPTPELWTLVLSHRTQILYVADISFVVMYLEIVPGCVVLESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRSGSARDDFEKIGISSLVTAGVRDIQGEGFPEEFAGLADAVFLDLPQPWLAIPSAAKMLRHDGTLCSFSPCIEQVQRSCETMQSNFTDIRTFEVLLRTYEVREGKMESLEINGDDGSNGSLPCKRRKRSDGASVVTYPNSSSVMARPCGEARGHTGYLTFARLN*