AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0305550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0305550 MtrunA17_Chr2g0333771 98.387 310 5 0 1 310 1 310 0.0 619
Msa0305550 MtrunA17_Chr4g0001221 83.548 310 43 3 4 310 1 305 0.0 506
Msa0305550 MtrunA17_Chr4g0001111 83.784 222 28 3 4 222 1 217 1.40e-128 365
Msa0305550 MtrunA17_Chr1g0201331 58.224 304 107 4 11 310 13 300 3.45e-110 322
Msa0305550 MtrunA17_Chr4g0001161 83.571 140 23 0 93 232 1 140 1.29e-77 233
Msa0305550 MtrunA17_Chr8g0379901 48.473 262 130 2 45 303 4 263 1.18e-76 235
Msa0305550 MtrunA17_Chr8g0379911 51.542 227 105 2 80 303 38 262 1.11e-74 230
Msa0305550 MtrunA17_Chr2g0284631 47.710 262 133 2 53 310 64 325 4.71e-74 230
Msa0305550 MtrunA17_Chr2g0284591 49.206 252 119 3 56 303 14 260 6.24e-73 226
Msa0305550 MtrunA17_Chr2g0284611 49.206 252 119 3 56 303 16 262 2.48e-72 224
Msa0305550 MtrunA17_Chr7g0217331 46.121 232 122 1 75 306 32 260 1.50e-71 222
Msa0305550 MtrunA17_Chr2g0284581 51.584 221 102 2 76 293 34 252 1.70e-69 217
Msa0305550 MtrunA17_Chr2g0284601 50.485 206 97 2 91 293 1 204 1.31e-61 195
Msa0305550 MtrunA17_Chr4g0001151 77.863 131 14 1 93 223 1 116 2.47e-60 195
Msa0305550 MtrunA17_Chr4g0044201 42.045 264 128 5 56 310 8 255 4.12e-60 192
Msa0305550 MtrunA17_Chr5g0435051 45.495 222 116 3 76 293 36 256 1.99e-57 186
Msa0305550 MtrunA17_Chr3g0079421 47.685 216 109 3 76 289 22 235 2.37e-55 180
Msa0305550 MtrunA17_Chr5g0426131 43.779 217 116 2 80 292 37 251 4.14e-55 180
Msa0305550 MtrunA17_Chr1g0184541 40.175 229 137 0 78 306 60 288 4.95e-51 170
Msa0305550 MtrunA17_Chr4g0001121 82.022 89 16 0 222 310 10 98 2.14e-43 144
Msa0305550 MtrunA17_Chr2g0331261 39.286 224 116 8 84 293 24 241 2.47e-32 120
Msa0305550 MtrunA17_Chr1g0146161 37.073 205 109 7 85 277 24 220 4.79e-29 111
Msa0305550 MtrunA17_Chr8g0340161 35.749 207 115 4 84 277 23 224 3.10e-27 106
Msa0305550 MtrunA17_Chr1g0188771 34.404 218 135 3 84 293 25 242 4.27e-26 103
Msa0305550 MtrunA17_Chr1g0146171 36.098 205 111 8 85 277 24 220 5.33e-25 100
Msa0305550 MtrunA17_Chr6g0478191 32.287 223 143 3 84 298 25 247 2.00e-24 99.4
Msa0305550 MtrunA17_Chr2g0326621 31.120 241 132 7 71 278 22 261 1.86e-23 97.4
Msa0305550 MtrunA17_Chr5g0399341 34.783 207 116 4 84 277 23 223 3.89e-23 95.9
Msa0305550 MtrunA17_Chr3g0145671 30.453 243 142 7 73 288 19 261 6.69e-23 95.9
Msa0305550 MtrunA17_Chr4g0030861 33.168 202 127 4 85 278 26 227 1.28e-21 91.7
Msa0305550 MtrunA17_Chr1g0212671 29.075 227 153 3 84 302 25 251 5.66e-21 90.1
Msa0305550 MtrunA17_Chr7g0264111 28.452 239 140 7 71 278 21 259 1.10e-18 84.3
Msa0305550 MtrunA17_Chr7g0264961 33.010 206 121 6 84 277 25 225 1.47e-18 83.2
Msa0305550 MtrunA17_Chr3g0113211 29.508 244 144 8 73 288 37 280 2.84e-18 83.2
Msa0305550 MtrunA17_Chr8g0386961 29.487 234 137 8 73 278 34 267 9.94e-18 81.6
Msa0305550 MtrunA17_Chr4g0006001 29.717 212 131 7 84 278 60 270 1.38e-16 78.6
Msa0305550 MtrunA17_Chr5g0397391 30.189 212 131 6 84 278 56 267 2.02e-16 78.2
Msa0305550 MtrunA17_Chr4g0029071 27.197 239 141 7 73 278 21 259 2.35e-16 77.8
Msa0305550 MtrunA17_Chr7g0222791 27.753 227 156 4 84 302 25 251 9.76e-16 75.5
Msa0305550 MtrunA17_Chr5g0436701 28.692 237 135 8 73 278 37 270 2.74e-15 74.7
Msa0305550 MtrunA17_Chr1g0199731 26.778 239 142 7 73 278 21 259 4.26e-15 74.3
Msa0305550 MtrunA17_Chr4g0020781 29.648 199 130 5 111 303 15 209 1.58e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0305550 AT1G80760.1 77.564 312 58 4 4 310 1 305 6.75e-167 466
Msa0305550 AT4G10380.1 67.416 267 75 3 46 310 48 304 5.13e-119 344
Msa0305550 AT5G37820.1 46.484 256 129 4 52 303 16 267 8.23e-72 224
Msa0305550 AT5G37820.2 46.484 256 129 4 52 303 52 303 1.24e-71 224
Msa0305550 AT4G19030.1 43.686 293 151 5 29 309 2 292 1.56e-70 221
Msa0305550 AT5G37810.1 46.964 247 125 3 59 303 25 267 5.87e-70 219
Msa0305550 AT4G18910.1 41.472 299 146 4 29 309 2 289 2.88e-69 218
Msa0305550 AT1G31885.1 45.600 250 128 2 68 309 31 280 2.60e-66 211
Msa0305550 AT1G31885.2 46.903 226 112 2 92 309 1 226 2.95e-61 196
Msa0305550 AT2G34390.1 40.217 276 148 5 46 310 13 282 1.84e-54 179
Msa0305550 AT2G34390.2 42.083 240 123 4 81 310 7 240 1.53e-51 170
Msa0305550 AT4G19030.3 44.000 225 112 5 29 241 2 224 2.61e-49 164
Msa0305550 AT4G18910.2 42.241 232 105 4 29 242 2 222 2.05e-48 161
Msa0305550 AT3G06100.1 38.208 212 130 1 80 290 46 257 1.50e-43 150
Msa0305550 AT3G06100.2 38.389 211 129 1 84 293 2 212 6.14e-43 147
Msa0305550 AT4G19030.2 41.748 206 106 5 29 222 2 205 1.53e-38 136
Msa0305550 AT2G34390.3 38.942 208 113 4 46 242 13 217 9.26e-35 126
Msa0305550 AT3G16240.1 37.198 207 112 5 84 277 23 224 1.16e-29 113
Msa0305550 AT2G34390.4 37.368 190 105 4 46 224 13 199 8.82e-28 108
Msa0305550 AT2G25810.1 35.885 209 116 7 80 277 19 220 3.40e-26 104
Msa0305550 AT5G47450.1 34.783 230 124 7 84 292 23 247 3.77e-26 104
Msa0305550 AT2G29870.1 46.957 115 58 1 196 310 22 133 1.12e-25 100
Msa0305550 AT4G17340.1 33.816 207 119 4 84 277 23 224 2.63e-24 99.4
Msa0305550 AT3G26520.1 34.783 207 117 6 84 277 26 227 7.96e-23 95.5
Msa0305550 AT2G36830.1 34.783 207 117 6 84 277 25 226 8.65e-23 95.5
Msa0305550 AT4G35100.1 30.864 243 141 7 73 288 20 262 4.12e-22 94.0
Msa0305550 AT4G35100.2 30.864 243 141 7 73 288 20 262 4.12e-22 94.0
Msa0305550 AT2G16850.1 30.328 244 141 7 73 288 18 260 4.74e-22 94.0
Msa0305550 AT5G60660.1 28.689 244 141 7 71 282 21 263 1.73e-20 89.7
Msa0305550 AT3G06100.3 34.532 139 90 1 156 293 41 179 7.77e-20 85.9
Msa0305550 AT3G54820.1 30.833 240 133 7 71 278 20 258 1.93e-19 87.0
Msa0305550 AT1G73190.1 30.493 223 142 4 84 293 28 250 1.43e-18 84.0
Msa0305550 AT4G01470.1 30.275 218 134 5 84 288 25 237 2.32e-18 83.2
Msa0305550 AT1G17810.1 29.956 227 142 6 84 295 28 252 8.86e-18 82.0
Msa0305550 AT2G37170.1 27.308 260 145 8 51 278 10 257 1.36e-17 81.6
Msa0305550 AT3G53420.1 27.917 240 140 7 71 278 21 259 1.62e-17 81.6
Msa0305550 AT3G53420.2 27.917 240 140 7 71 278 21 259 1.62e-17 81.6
Msa0305550 AT2G37170.2 27.308 260 145 8 51 278 53 300 3.02e-17 81.3
Msa0305550 AT2G45960.1 31.915 235 129 9 73 278 34 266 8.17e-17 79.3
Msa0305550 AT2G37180.1 26.923 260 146 8 51 278 10 257 1.09e-16 79.0
Msa0305550 AT4G23400.1 30.189 212 130 6 84 278 57 267 1.32e-16 79.0
Msa0305550 AT4G00430.1 29.487 234 136 8 73 278 35 267 1.45e-16 79.0
Msa0305550 AT1G31885.3 41.758 91 53 0 66 156 29 119 2.05e-16 75.1
Msa0305550 AT1G01620.1 30.660 212 129 6 84 278 56 266 2.45e-16 78.2
Msa0305550 AT2G39010.1 28.333 240 137 7 73 278 20 258 2.56e-16 78.2
Msa0305550 AT3G61430.2 31.455 213 126 7 84 278 56 266 3.38e-16 77.8
Msa0305550 AT3G61430.1 31.455 213 126 7 84 278 56 266 3.38e-16 77.8
Msa0305550 AT3G47440.1 27.586 232 151 5 84 303 27 253 2.35e-15 74.7
Msa0305550 AT1G01620.2 32.402 179 106 5 114 278 17 194 1.41e-14 72.0
Msa0305550 AT2G45960.3 30.594 219 126 7 73 266 34 251 2.09e-14 72.8
Msa0305550 AT2G45960.2 31.818 220 122 8 73 266 34 251 2.58e-14 72.0

Find 191 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAATCATGATTAATTAAT+TGG 0.122870 2_3:-63288441 Msa0305550:five_prime_UTR
TGCACATGGCCAAATGATTT+TGG 0.183955 2_3:+63286333 None:intergenic
TTCATTGACGCTCCTGTTAT+TGG 0.213047 2_3:+63286657 None:intergenic
CATCTTCTAAGTTCCATTCT+TGG 0.216409 2_3:+63287838 None:intergenic
GTGACTCCACCACTCATAAA+AGG 0.246044 2_3:+63287152 None:intergenic
AGTAACTAATTGTTGTTTAC+AGG 0.248708 2_3:+63288513 None:intergenic
CTTACTAGACTCTGGAAATT+AGG 0.255989 2_3:+63288276 None:intergenic
GTTTGATTTCATCACCATTC+AGG 0.261433 2_3:+63285485 None:intergenic
CTACAAACTTGCAGAAATTA+AGG 0.267685 2_3:-63288385 Msa0305550:five_prime_UTR
GCGATGATGCGGAGACGTTT+TGG 0.292273 2_3:-63285429 Msa0305550:three_prime_UTR
TCTCCGAGCCTTGTGTCTTT+TGG 0.294166 2_3:+63287536 None:intergenic
CTTTGCATTATCATCTTCTT+CGG 0.298809 2_3:+63286512 None:intergenic
CAGATAGGAGCCGAGTTTAT+AGG 0.306620 2_3:-63287604 Msa0305550:CDS
GAAACTGATATATGAGAAAA+AGG 0.310726 2_3:+63288209 None:intergenic
TGTGCCATTGCTCAAGAATT+AGG 0.311160 2_3:+63288556 None:intergenic
CTCTTCCGGTGCTTCCTTCA+AGG 0.319707 2_3:+63285346 None:intergenic
CCAGGAGTGCCTGGTGTTGC+TGG 0.322227 2_3:+63287957 None:intergenic
CCTGGTGTTGCTGGTGTTGA+TGG 0.322832 2_3:+63287966 None:intergenic
CTCGGCTATTTCCGCTCTTC+CGG 0.331339 2_3:+63285332 None:intergenic
CACTAGGCATCAATTATATA+CGG 0.333235 2_3:+63288320 None:intergenic
GTATATTGGTGCACAAGTTT+TGG 0.339591 2_3:-63287216 Msa0305550:CDS
TTTACCATTGACTGTAGAAA+TGG 0.347231 2_3:-63288012 Msa0305550:exon
AGATAGGAGCCGAGTTTATA+GGG 0.358525 2_3:-63287603 Msa0305550:CDS
GCTGGACCTAGAGTTCTTAC+TGG 0.377381 2_3:+63286633 None:intergenic
CTGCGTTGCAGGCCAATAAC+AGG 0.388557 2_3:-63286669 Msa0305550:intron
CTCTCCACCGGTCACATCTC+TGG 0.392092 2_3:-63287463 Msa0305550:CDS
ACCGTTCCTTCCGGAGGATA+TGG 0.393092 2_3:-63287131 Msa0305550:CDS
GAGTTGAACTTACGTCTTTG+TGG 0.400319 2_3:+63285991 None:intergenic
ATTAGAGATAATTAATTACT+TGG 0.401264 2_3:+63286136 None:intergenic
TAGTTATTTGCAGCTATAGC+TGG 0.401428 2_3:+63286615 None:intergenic
ATTGGGTGTGCTACCTCTAC+TGG 0.401500 2_3:-63287508 Msa0305550:CDS
CAAATAACTACAAAGCCATA+TGG 0.404031 2_3:-63286602 Msa0305550:CDS
ACATTGATTCTGATGTTTGC+TGG 0.409739 2_3:-63287580 Msa0305550:CDS
AATTGAGTGCTCTTTCAGTT+TGG 0.411453 2_3:-63286234 Msa0305550:three_prime_UTR
AATTTACATTAGACAAGAAA+AGG 0.411623 2_3:+63288033 None:intergenic
CAAGGCTCGGAGACTCTGAT+TGG 0.413496 2_3:-63287526 Msa0305550:CDS
TATTGTTGCTATTGTATGTC+GGG 0.414071 2_3:+63288092 None:intergenic
TTTGCATTATCATCTTCTTC+GGG 0.414167 2_3:+63286513 None:intergenic
TGCAAGAGGCACAGGAGGAG+GGG 0.422658 2_3:+63287779 None:intergenic
TTTCCATTCATCAATTTCCT+TGG 0.427402 2_3:+63288344 None:intergenic
GGCGGCTTCTTTAGCGGAGC+CGG 0.433073 2_3:-63285303 Msa0305550:three_prime_UTR
CATTGATTCTGATGTTTGCT+GGG 0.434089 2_3:-63287579 Msa0305550:CDS
CTCAAGAATTGTAAATGCTT+CGG 0.434143 2_3:-63287864 Msa0305550:CDS
TGTTCCATTTCTACAGTCAA+TGG 0.435070 2_3:+63288008 None:intergenic
CCACCTAGAGCTCCAAGTAT+TGG 0.435294 2_3:+63286564 None:intergenic
GGTGGAGTCACCGTTCCTTC+CGG 0.437337 2_3:-63287140 Msa0305550:CDS
CTCCATTCTTTCACACAACT+CGG 0.440601 2_3:-63285117 Msa0305550:three_prime_UTR
GTGCTGCATTTGCTCTCAAA+GGG 0.443470 2_3:-63287184 Msa0305550:CDS
TTTGCAAGAGGCACAGGAGG+AGG 0.446500 2_3:+63287777 None:intergenic
TATCTCTTAGCTCCAATACT+TGG 0.455159 2_3:-63286576 Msa0305550:CDS
TAGCATGACTAGTATCACTA+TGG 0.456539 2_3:+63288160 None:intergenic
AACCGAGTTGTGTGAAAGAA+TGG 0.458481 2_3:+63285115 None:intergenic
AGTTGATCATCTTCTAAAGC+TGG 0.459544 2_3:+63286467 None:intergenic
ATTAGCAACAGATAAAATAT+TGG 0.459980 2_3:-63286097 Msa0305550:three_prime_UTR
TTGCAGGTGCCTATGTATAT+TGG 0.461576 2_3:-63287230 Msa0305550:intron
AATACACTCTCATGATACTT+AGG 0.463385 2_3:-63288470 Msa0305550:five_prime_UTR
TTAATTATCTCTAATTACAT+AGG 0.466666 2_3:-63286128 Msa0305550:three_prime_UTR
ATCAGCAATGGCGTCCTTGA+AGG 0.467946 2_3:-63285360 Msa0305550:three_prime_UTR
CTTCAATGCAGCAAAAGAAA+TGG 0.468532 2_3:+63287417 None:intergenic
AGTTGAACTTACGTCTTTGT+GGG 0.469754 2_3:+63285992 None:intergenic
ATGCTCAATATACTAATAGC+AGG 0.472157 2_3:-63286788 Msa0305550:CDS
TTGCAAGAGGCACAGGAGGA+GGG 0.478192 2_3:+63287778 None:intergenic
CAGTGTTGGGACTCGTTGGC+GGG 0.479451 2_3:-63285965 Msa0305550:three_prime_UTR
TGGAAATGGCGTGGCGGCGG+AGG 0.479809 2_3:-63285256 Msa0305550:three_prime_UTR
AGAGGTACACCAGGAGTGCC+TGG 0.482854 2_3:+63287948 None:intergenic
AGGGAAATGCCTTGAGATTG+AGG 0.484724 2_3:-63285461 Msa0305550:three_prime_UTR
ATGGCGTGGCGGCGGAGGAA+AGG 0.488153 2_3:-63285251 Msa0305550:three_prime_UTR
CCAGTGTTGGGACTCGTTGG+CGG 0.490261 2_3:-63285966 Msa0305550:three_prime_UTR
AAATAACTACAAAGCCATAT+GGG 0.491686 2_3:-63286601 Msa0305550:CDS
AATGCTTCGGTGTCCAAGAA+TGG 0.493128 2_3:-63287851 Msa0305550:CDS
AACTCTTGATTAGAAAGAGC+TGG 0.493233 2_3:-63286050 Msa0305550:three_prime_UTR
GAAATTAAGGAGAACACAAA+AGG 0.495825 2_3:-63288372 Msa0305550:five_prime_UTR
CTTGGAGCTCTAGGTGGGGC+AGG 0.496173 2_3:-63286558 Msa0305550:CDS
GCAAGAGGCACAGGAGGAGG+GGG 0.497170 2_3:+63287780 None:intergenic
GCATTTACAATTCTTGAGTA+GGG 0.497185 2_3:+63287869 None:intergenic
CGATGGAAATGGCGTGGCGG+CGG 0.499487 2_3:-63285259 Msa0305550:three_prime_UTR
CGCCGCCACGCCATTTCCAT+CGG 0.499717 2_3:+63285260 None:intergenic
TACATGGTTAAAGTAAAAGA+TGG 0.501830 2_3:-63286171 Msa0305550:three_prime_UTR
GCAAAAGAAATGGTGACTGC+AGG 0.502542 2_3:+63287427 None:intergenic
AATGAAAGGAAGCAAATTAG+TGG 0.504370 2_3:+63288184 None:intergenic
GAGAGAAACGACGGATCCGA+TGG 0.506821 2_3:-63285276 Msa0305550:three_prime_UTR
AACGACGGATCCGATGGAAA+TGG 0.510472 2_3:-63285270 Msa0305550:three_prime_UTR
AGTTCAACTCTGCCAGTGTT+GGG 0.512112 2_3:-63285978 Msa0305550:three_prime_UTR
CAAGAATGGAACTTAGAAGA+TGG 0.514046 2_3:-63287837 Msa0305550:CDS
CCAGCAACACCAGGCACTCC+TGG 0.515961 2_3:-63287957 Msa0305550:CDS
GTTGATCATCTTCTAAAGCT+GGG 0.517734 2_3:+63286468 None:intergenic
TCATCATAAAATGACACTAA+AGG 0.518324 2_3:-63286421 Msa0305550:three_prime_UTR
AAAATATTCACATACCTGAA+TGG 0.519388 2_3:-63285499 Msa0305550:intron
AATGGAACATATTAATAATG+AGG 0.519868 2_3:-63287994 Msa0305550:CDS
ATGAACCCAGTAAGAACTCT+AGG 0.522426 2_3:-63286639 Msa0305550:CDS
TAATCTAATGTTCGTTGTCA+CGG 0.524885 2_3:-63287075 Msa0305550:CDS
GCGGAAATAGCCGAGGTGAT+CGG 0.524946 2_3:-63285324 Msa0305550:three_prime_UTR
AAGGTAGCAGCATGTGGAAG+TGG 0.525892 2_3:+63288228 None:intergenic
GACTCTGGAAATTAGGAAAG+AGG 0.528630 2_3:+63288283 None:intergenic
TTATCCTAATTCTTGAGCAA+TGG 0.528782 2_3:-63288560 Msa0305550:five_prime_UTR
TGACCAAGGAAATTGATGAA+TGG 0.530797 2_3:-63288347 Msa0305550:five_prime_UTR
TTATTGTTGCTATTGTATGT+CGG 0.531372 2_3:+63288091 None:intergenic
GCTCCAATACTTGGAGCTCT+AGG 0.532633 2_3:-63286567 Msa0305550:CDS
AATTTGTCTGCGCCTAACCA+TGG 0.534953 2_3:-63285161 Msa0305550:three_prime_UTR
AGCATTTACAATTCTTGAGT+AGG 0.536216 2_3:+63287868 None:intergenic
CTTCTTTAGCGGAGCCGGCG+AGG 0.536281 2_3:-63285298 Msa0305550:three_prime_UTR
GTGATCGGCGGCTTCTTTAG+CGG 0.537566 2_3:-63285309 Msa0305550:three_prime_UTR
TGACTCCACCACTCATAAAA+GGG 0.537986 2_3:+63287153 None:intergenic
CTGCCAGTGTTGGGACTCGT+TGG 0.540233 2_3:-63285969 Msa0305550:three_prime_UTR
GGCGTCCTTGAAGGAAGCAC+CGG 0.547572 2_3:-63285351 Msa0305550:three_prime_UTR
GCTTTGGTTACATGCTTCCA+CGG 0.548483 2_3:+63287388 None:intergenic
TTATGATGATTGATGAGCAT+GGG 0.550009 2_3:+63286435 None:intergenic
TGTGCTGCATTTGCTCTCAA+AGG 0.550088 2_3:-63287185 Msa0305550:CDS
GTATATCTAATTCGTGCACA+TGG 0.551064 2_3:+63286319 None:intergenic
AAGGCTCGGAGACTCTGATT+GGG 0.552417 2_3:-63287525 Msa0305550:CDS
TTTATGATGATTGATGAGCA+TGG 0.552805 2_3:+63286434 None:intergenic
GGTTTAAGTCTGAGAGAAGC+GGG 0.553612 2_3:-63287917 Msa0305550:CDS
ATTTACATTAGACAAGAAAA+GGG 0.554099 2_3:+63288034 None:intergenic
AATGTTCGTTGTCACGGCAG+TGG 0.554743 2_3:-63287069 Msa0305550:CDS
AAGTCTGAGAGAAGCGGGAA+TGG 0.555069 2_3:-63287912 Msa0305550:CDS
ATTTGTCTGCGCCTAACCAT+GGG 0.556322 2_3:-63285160 Msa0305550:three_prime_UTR
AGGGGACTCTTACTAGACTC+TGG 0.557413 2_3:+63288268 None:intergenic
CTGGACCTAGAGTTCTTACT+GGG 0.557635 2_3:+63286634 None:intergenic
TGCTACCTCTACTGGTCTTG+CGG 0.560160 2_3:-63287500 Msa0305550:CDS
CAATACTTGGAGCTCTAGGT+GGG 0.562103 2_3:-63286563 Msa0305550:CDS
AAATTAGAGTACAGAAGCAC+GGG 0.563522 2_3:+63288061 None:intergenic
TCTCATCCTCCTCAATCTCA+AGG 0.564063 2_3:+63285452 None:intergenic
GTAACTAATTGTTGTTTACA+GGG 0.565538 2_3:+63288514 None:intergenic
GGACGGTGGGACAGTTCTGA+GGG 0.568449 2_3:+63288249 None:intergenic
AAGTTCAACTCTGCCAGTGT+TGG 0.569468 2_3:-63285979 Msa0305550:three_prime_UTR
GAACTAGCAGGAATTGCAGT+GGG 0.569469 2_3:-63286821 Msa0305550:CDS
CCAATACTTGGAGCTCTAGG+TGG 0.572700 2_3:-63286564 Msa0305550:CDS
ACCATATCCTCCGGAAGGAA+CGG 0.576185 2_3:+63287130 None:intergenic
AGCTAAGAGATAGACCCATA+TGG 0.576739 2_3:+63286587 None:intergenic
CCATCAACACCAGCAACACC+AGG 0.577155 2_3:-63287966 Msa0305550:CDS
TGGACGGTGGGACAGTTCTG+AGG 0.577190 2_3:+63288248 None:intergenic
CTGCATTGAAGCACTTCCCG+TGG 0.578067 2_3:-63287405 Msa0305550:CDS
CTTCACTCACTTAAAGAGCA+TGG 0.578332 2_3:+63288418 None:intergenic
CAGAGATGTGACCGGTGGAG+AGG 0.581423 2_3:+63287464 None:intergenic
TGGAAATTAGGAAAGAGGCT+TGG 0.583275 2_3:+63288288 None:intergenic
AGTATCACTATGGAAATGAA+AGG 0.584129 2_3:+63288170 None:intergenic
CATAACCGCAAGACCAGTAG+AGG 0.586587 2_3:+63287495 None:intergenic
CCAGGTCAGGGTAAGCCTCA+AGG 0.587542 2_3:+63285928 None:intergenic
CATTAATGAGCAGGTAACTG+TGG 0.587885 2_3:+63287806 None:intergenic
CAAAAGAAATGGTGACTGCA+GGG 0.592708 2_3:+63287428 None:intergenic
ATGATCATCATCCTCTCCAC+CGG 0.597115 2_3:-63287475 Msa0305550:CDS
AAAAGCTTGACCATATCCTC+CGG 0.599263 2_3:+63287121 None:intergenic
ACTTGTGCACCAATATACAT+AGG 0.600697 2_3:+63287221 None:intergenic
AATCAATGTCCCTATAAACT+CGG 0.603006 2_3:+63287594 None:intergenic
TGAAGGAAGCACCGGAAGAG+CGG 0.603647 2_3:-63285343 Msa0305550:three_prime_UTR
GAAGACACAGACCCATGGTT+AGG 0.608517 2_3:+63285149 None:intergenic
GAAATTAGAGTACAGAAGCA+CGG 0.609449 2_3:+63288060 None:intergenic
CTTTGGTTACATGCTTCCAC+GGG 0.610743 2_3:+63287389 None:intergenic
CCGCCAACGAGTCCCAACAC+TGG 0.619707 2_3:+63285966 None:intergenic
TGCTGCATTTGCTCTCAAAG+GGG 0.620449 2_3:-63287183 Msa0305550:CDS
GGAGTCACCGTTCCTTCCGG+AGG 0.622617 2_3:-63287137 Msa0305550:CDS
ACTCACAGCTCTTGTGTCGG+TGG 0.623869 2_3:+63287046 None:intergenic
GGGTTTAAGTCTGAGAGAAG+CGG 0.625558 2_3:-63287918 Msa0305550:CDS
ATGTAGGTAGGAGAACTAGC+AGG 0.625936 2_3:-63286833 Msa0305550:intron
ATTAATGAGCAGGTAACTGT+GGG 0.626119 2_3:+63287807 None:intergenic
TAGCAGCATGTGGAAGTGGA+CGG 0.626553 2_3:+63288232 None:intergenic
TCCGTCGTTTCTCTCCTCGC+CGG 0.628814 2_3:+63285284 None:intergenic
AGAACTAGCAGGAATTGCAG+TGG 0.628860 2_3:-63286822 Msa0305550:CDS
GCCGGCGAGGAGAGAAACGA+CGG 0.631923 2_3:-63285285 Msa0305550:three_prime_UTR
CATGCTCTTTAAGTGAGTGA+AGG 0.634040 2_3:-63288417 Msa0305550:five_prime_UTR
GTTGAACTTACGTCTTTGTG+GGG 0.634464 2_3:+63285993 None:intergenic
AGCATGTGGAAGTGGACGGT+GGG 0.635263 2_3:+63288236 None:intergenic
AATGGTGATGAAATCAAACA+AGG 0.637548 2_3:-63285481 Msa0305550:three_prime_UTR
CGGATCCGATGGAAATGGCG+TGG 0.638641 2_3:-63285265 Msa0305550:three_prime_UTR
GGCTTGGTTCACACTACACT+AGG 0.639934 2_3:+63288304 None:intergenic
GACGCCATTGCTGATATCGT+CGG 0.640160 2_3:+63285368 None:intergenic
GGAGGATGAGAGCGATGATG+CGG 0.640572 2_3:-63285440 Msa0305550:three_prime_UTR
GCAATCTGCTATGATCCTTG+AGG 0.641389 2_3:-63285943 Msa0305550:three_prime_UTR
TTGATCATCTTCTAAAGCTG+GGG 0.642052 2_3:+63286469 None:intergenic
TCGGAAAACAGAAAGAGCGA+AGG 0.646416 2_3:-63285405 Msa0305550:three_prime_UTR
GAACCAAAAGACACAAGGCT+CGG 0.650309 2_3:-63287539 Msa0305550:CDS
GCTTGACCATATCCTCCGGA+AGG 0.651188 2_3:+63287125 None:intergenic
TATGATGATTGATGAGCATG+GGG 0.652693 2_3:+63286436 None:intergenic
CAGCATGTGGAAGTGGACGG+TGG 0.655771 2_3:+63288235 None:intergenic
AAGATGAAGACACAGACCCA+TGG 0.655950 2_3:+63285144 None:intergenic
AATACTTGGAGCTCTAGGTG+GGG 0.656773 2_3:-63286562 Msa0305550:CDS
AGCACCAGAGATGTGACCGG+TGG 0.657407 2_3:+63287459 None:intergenic
TGCTCAATATACTAATAGCA+GGG 0.657754 2_3:-63286787 Msa0305550:intron
ATGAGCACCAGAGATGTGAC+CGG 0.661506 2_3:+63287456 None:intergenic
GAACACAAAAGGAATGACCA+AGG 0.662028 2_3:-63288361 Msa0305550:five_prime_UTR
ATGGTGATGAAATCAAACAA+GGG 0.663795 2_3:-63285480 Msa0305550:three_prime_UTR
AACACCGACGATATCAGCAA+TGG 0.665392 2_3:-63285372 Msa0305550:three_prime_UTR
ATCCGATGGAAATGGCGTGG+CGG 0.667955 2_3:-63285262 Msa0305550:three_prime_UTR
GAAATAGCCGAGGTGATCGG+CGG 0.670432 2_3:-63285321 Msa0305550:three_prime_UTR
AATACTCACAGCTCTTGTGT+CGG 0.671145 2_3:+63287043 None:intergenic
CGGAAGAGCGGAAATAGCCG+AGG 0.678071 2_3:-63285331 Msa0305550:three_prime_UTR
AAAGAAGCCGCCGATCACCT+CGG 0.698591 2_3:+63285314 None:intergenic
ATTGTGAACCAAAAGACACA+AGG 0.703696 2_3:-63287544 Msa0305550:CDS
GACGGTGGGACAGTTCTGAG+GGG 0.726625 2_3:+63288250 None:intergenic
GAAATGCCTTGAGATTGAGG+AGG 0.735482 2_3:-63285458 Msa0305550:three_prime_UTR
ATGATGATTGATGAGCATGG+GGG 0.825649 2_3:+63286437 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATCATGATTAATTAAT+TGG - chr2_3:63285265-63285284 Msa0305550:three_prime_UTR 10.0%
!! TAATTTCAACAACTTTAATT+AGG + chr2_3:63286370-63286389 None:intergenic 15.0%
!! AAAGTATAAATTATAATTGC+AGG - chr2_3:63286460-63286479 Msa0305550:exon 15.0%
!!! CATATGTTCAAATTTTAATT+TGG + chr2_3:63286788-63286807 None:intergenic 15.0%
!!! ATATATATATAAGTTTTGTG+TGG - chr2_3:63287338-63287357 Msa0305550:intron 15.0%
!! ATTAGAGATAATTAATTACT+TGG + chr2_3:63287573-63287592 None:intergenic 15.0%
!! TTAATTATCTCTAATTACAT+AGG - chr2_3:63287578-63287597 Msa0305550:CDS 15.0%
!! AATCATATATATATAGTGTT+TGG + chr2_3:63288183-63288202 None:intergenic 15.0%
!! ATTTACATTAGACAAGAAAA+GGG + chr2_3:63285675-63285694 None:intergenic 20.0%
!! AATTTACATTAGACAAGAAA+AGG + chr2_3:63285676-63285695 None:intergenic 20.0%
!! AATGGAACATATTAATAATG+AGG - chr2_3:63285712-63285731 Msa0305550:intron 20.0%
!! ACAACTTTAATTAGGAAATT+AGG + chr2_3:63286362-63286381 None:intergenic 20.0%
!! TATTAGCTAAAAGTTACTAT+AGG + chr2_3:63286693-63286712 None:intergenic 20.0%
!! TTAAAATTTGAACATATGCT+TGG - chr2_3:63286790-63286809 Msa0305550:CDS 20.0%
!! ATAACCATAAACATCAATTA+GGG + chr2_3:63286855-63286874 None:intergenic 20.0%
!!! AATATTTTATCTGTTGCTAA+TGG + chr2_3:63287610-63287629 None:intergenic 20.0%
!! ATTAGCAACAGATAAAATAT+TGG - chr2_3:63287609-63287628 Msa0305550:intron 20.0%
!! TTTCAAAGTAAAGTTATTGA+TGG - chr2_3:63287879-63287898 Msa0305550:CDS 20.0%
!! TTCAAAGTAAAGTTATTGAT+GGG - chr2_3:63287880-63287899 Msa0305550:CDS 20.0%
!!! CATTTTTCATTAGCTTTTTT+CGG - chr2_3:63288010-63288029 Msa0305550:exon 20.0%
!! ATATATAGTGTTTGGATAAA+CGG + chr2_3:63288175-63288194 None:intergenic 20.0%
!!! GTAACTAATTGTTGTTTACA+GGG + chr2_3:63285195-63285214 None:intergenic 25.0%
!!! AGTAACTAATTGTTGTTTAC+AGG + chr2_3:63285196-63285215 None:intergenic 25.0%
! GAAACTGATATATGAGAAAA+AGG + chr2_3:63285500-63285519 None:intergenic 25.0%
!! TTATTGTTGCTATTGTATGT+CGG + chr2_3:63285618-63285637 None:intergenic 25.0%
!!! ATATATACCTTTTTTGCAAG+AGG + chr2_3:63285944-63285963 None:intergenic 25.0%
! ATTTATGTGTGTCTAATCTT+TGG - chr2_3:63286002-63286021 Msa0305550:three_prime_UTR 25.0%
! AGTTTGAAAAGAAAAAGAGA+AGG - chr2_3:63286731-63286750 Msa0305550:intron 25.0%
! CATAACCATAAACATCAATT+AGG + chr2_3:63286856-63286875 None:intergenic 25.0%
! ATTGATGTTTATGGTTATGT+AGG - chr2_3:63286857-63286876 Msa0305550:intron 25.0%
! AAATAACTACAAAGCCATAT+GGG - chr2_3:63287105-63287124 Msa0305550:CDS 25.0%
! TCATCATAAAATGACACTAA+AGG - chr2_3:63287285-63287304 Msa0305550:intron 25.0%
! AAAAACAAGCCAAAATCATT+TGG - chr2_3:63287364-63287383 Msa0305550:intron 25.0%
!! TCTTTTACTTTAACCATGTA+AGG + chr2_3:63287535-63287554 None:intergenic 25.0%
! TACATGGTTAAAGTAAAAGA+TGG - chr2_3:63287535-63287554 Msa0305550:CDS 25.0%
!! ACCTAAACTTTTCTTATTGA+AGG - chr2_3:63287820-63287839 Msa0305550:CDS 25.0%
! TCCTTCAATAAGAAAAGTTT+AGG + chr2_3:63287824-63287843 None:intergenic 25.0%
! TCTTATTGAAGGAAGAAAAA+AGG - chr2_3:63287831-63287850 Msa0305550:CDS 25.0%
! CTATAGCAAAAGATAAAATG+AGG + chr2_3:63288103-63288122 None:intergenic 25.0%
! AAAATATTCACATACCTGAA+TGG - chr2_3:63288207-63288226 Msa0305550:five_prime_UTR 25.0%
TTATCCTAATTCTTGAGCAA+TGG - chr2_3:63285146-63285165 Msa0305550:three_prime_UTR 30.0%
AATACACTCTCATGATACTT+AGG - chr2_3:63285236-63285255 Msa0305550:three_prime_UTR 30.0%
CTACAAACTTGCAGAAATTA+AGG - chr2_3:63285321-63285340 Msa0305550:three_prime_UTR 30.0%
GAAATTAAGGAGAACACAAA+AGG - chr2_3:63285334-63285353 Msa0305550:three_prime_UTR 30.0%
TTTCCATTCATCAATTTCCT+TGG + chr2_3:63285365-63285384 None:intergenic 30.0%
CACTAGGCATCAATTATATA+CGG + chr2_3:63285389-63285408 None:intergenic 30.0%
AATGAAAGGAAGCAAATTAG+TGG + chr2_3:63285525-63285544 None:intergenic 30.0%
AGTATCACTATGGAAATGAA+AGG + chr2_3:63285539-63285558 None:intergenic 30.0%
! TATTGTTGCTATTGTATGTC+GGG + chr2_3:63285617-63285636 None:intergenic 30.0%
! TTTACCATTGACTGTAGAAA+TGG - chr2_3:63285694-63285713 Msa0305550:intron 30.0%
GCATTTACAATTCTTGAGTA+GGG + chr2_3:63285840-63285859 None:intergenic 30.0%
AGCATTTACAATTCTTGAGT+AGG + chr2_3:63285841-63285860 None:intergenic 30.0%
CTCAAGAATTGTAAATGCTT+CGG - chr2_3:63285842-63285861 Msa0305550:intron 30.0%
TACTTAGCACTTCAATGATA+TGG + chr2_3:63286048-63286067 None:intergenic 30.0%
AATCAATGTCCCTATAAACT+CGG + chr2_3:63286115-63286134 None:intergenic 30.0%
TAATTAGGAAATTAGGCTGA+AGG + chr2_3:63286355-63286374 None:intergenic 30.0%
TAATCTAATGTTCGTTGTCA+CGG - chr2_3:63286631-63286650 Msa0305550:CDS 30.0%
ATGTTTATGGTTATGTAGGT+AGG - chr2_3:63286861-63286880 Msa0305550:intron 30.0%
ATGCTCAATATACTAATAGC+AGG - chr2_3:63286918-63286937 Msa0305550:intron 30.0%
TGCTCAATATACTAATAGCA+GGG - chr2_3:63286919-63286938 Msa0305550:intron 30.0%
TGTATATGTTCATGACAAGT+GGG + chr2_3:63287007-63287026 None:intergenic 30.0%
ATGTATATGTTCATGACAAG+TGG + chr2_3:63287008-63287027 None:intergenic 30.0%
TGTCATGAACATATACATGA+TGG - chr2_3:63287010-63287029 Msa0305550:intron 30.0%
CAAATAACTACAAAGCCATA+TGG - chr2_3:63287104-63287123 Msa0305550:CDS 30.0%
TTTGCATTATCATCTTCTTC+GGG + chr2_3:63287196-63287215 None:intergenic 30.0%
CTTTGCATTATCATCTTCTT+CGG + chr2_3:63287197-63287216 None:intergenic 30.0%
TTATGATGATTGATGAGCAT+GGG + chr2_3:63287274-63287293 None:intergenic 30.0%
TTTATGATGATTGATGAGCA+TGG + chr2_3:63287275-63287294 None:intergenic 30.0%
! CTATTTTCTTTCTCCTTACA+TGG - chr2_3:63287519-63287538 Msa0305550:CDS 30.0%
AAGAAAAGTTTAGGTGAGTT+GGG + chr2_3:63287815-63287834 None:intergenic 30.0%
TAAGAAAAGTTTAGGTGAGT+TGG + chr2_3:63287816-63287835 None:intergenic 30.0%
ATAAGTTATAACACCCTGTT+TGG - chr2_3:63287962-63287981 Msa0305550:CDS 30.0%
TAAGTTATAACACCCTGTTT+GGG - chr2_3:63287963-63287982 Msa0305550:CDS 30.0%
AGCTAATGAAAAATGTCACA+AGG + chr2_3:63288005-63288024 None:intergenic 30.0%
!! GTTGTTTTCATAAGCTATCT+TGG + chr2_3:63288054-63288073 None:intergenic 30.0%
! AATGGTGATGAAATCAAACA+AGG - chr2_3:63288225-63288244 Msa0305550:five_prime_UTR 30.0%
! ATGGTGATGAAATCAAACAA+GGG - chr2_3:63288226-63288245 Msa0305550:five_prime_UTR 30.0%
TGACCAAGGAAATTGATGAA+TGG - chr2_3:63285359-63285378 Msa0305550:three_prime_UTR 35.0%
! CTTACTAGACTCTGGAAATT+AGG + chr2_3:63285433-63285452 None:intergenic 35.0%
TAGCATGACTAGTATCACTA+TGG + chr2_3:63285549-63285568 None:intergenic 35.0%
! AAATTAGAGTACAGAAGCAC+GGG + chr2_3:63285648-63285667 None:intergenic 35.0%
GAAATTAGAGTACAGAAGCA+CGG + chr2_3:63285649-63285668 None:intergenic 35.0%
TGTTCCATTTCTACAGTCAA+TGG + chr2_3:63285701-63285720 None:intergenic 35.0%
CATCTTCTAAGTTCCATTCT+TGG + chr2_3:63285871-63285890 None:intergenic 35.0%
CAAGAATGGAACTTAGAAGA+TGG - chr2_3:63285869-63285888 Msa0305550:intron 35.0%
ATTAATGAGCAGGTAACTGT+GGG + chr2_3:63285902-63285921 None:intergenic 35.0%
! ATCTGTAACACAGGTAGTTT+TGG + chr2_3:63286087-63286106 None:intergenic 35.0%
AAACTACCTGTGTTACAGAT+AGG - chr2_3:63286087-63286106 Msa0305550:three_prime_UTR 35.0%
!! ACATTGATTCTGATGTTTGC+TGG - chr2_3:63286126-63286145 Msa0305550:three_prime_UTR 35.0%
!! CATTGATTCTGATGTTTGCT+GGG - chr2_3:63286127-63286146 Msa0305550:three_prime_UTR 35.0%
ATTGTGAACCAAAAGACACA+AGG - chr2_3:63286162-63286181 Msa0305550:three_prime_UTR 35.0%
CTTCAATGCAGCAAAAGAAA+TGG + chr2_3:63286292-63286311 None:intergenic 35.0%
GAAGGATTAAGGAAAAGCTT+TGG + chr2_3:63286337-63286356 None:intergenic 35.0%
GAAATTAGGCTGAAGGATTA+AGG + chr2_3:63286348-63286367 None:intergenic 35.0%
ACTTGTGCACCAATATACAT+AGG + chr2_3:63286488-63286507 None:intergenic 35.0%
! GTATATTGGTGCACAAGTTT+TGG - chr2_3:63286490-63286509 Msa0305550:CDS 35.0%
! GTTTTTCACCCTTTTATGAG+TGG - chr2_3:63286545-63286564 Msa0305550:CDS 35.0%
GATGATATTATCCCCTATGT+CGG + chr2_3:63286766-63286785 None:intergenic 35.0%
GAGTCCCTAATTGATGTTTA+TGG - chr2_3:63286848-63286867 Msa0305550:intron 35.0%
GTATATGTTCATGACAAGTG+GGG + chr2_3:63287006-63287025 None:intergenic 35.0%
TAGTTATTTGCAGCTATAGC+TGG + chr2_3:63287094-63287113 None:intergenic 35.0%
TATCTCTTAGCTCCAATACT+TGG - chr2_3:63287130-63287149 Msa0305550:CDS 35.0%
!! TTGATCATCTTCTAAAGCTG+GGG + chr2_3:63287240-63287259 None:intergenic 35.0%
!! GTTGATCATCTTCTAAAGCT+GGG + chr2_3:63287241-63287260 None:intergenic 35.0%
!! AGTTGATCATCTTCTAAAGC+TGG + chr2_3:63287242-63287261 None:intergenic 35.0%
TATGATGATTGATGAGCATG+GGG + chr2_3:63287273-63287292 None:intergenic 35.0%
GTATATCTAATTCGTGCACA+TGG + chr2_3:63287390-63287409 None:intergenic 35.0%
AATTGAGTGCTCTTTCAGTT+TGG - chr2_3:63287472-63287491 Msa0305550:CDS 35.0%
AACTCTTGATTAGAAAGAGC+TGG - chr2_3:63287656-63287675 Msa0305550:intron 35.0%
AGTTGAACTTACGTCTTTGT+GGG + chr2_3:63287717-63287736 None:intergenic 35.0%
! TTCACAAGTTTTCCCAAACA+GGG + chr2_3:63287978-63287997 None:intergenic 35.0%
! TTTCACAAGTTTTCCCAAAC+AGG + chr2_3:63287979-63287998 None:intergenic 35.0%
TTCATAAGCTATCTTGGAGA+AGG + chr2_3:63288048-63288067 None:intergenic 35.0%
! GTTTGATTTCATCACCATTC+AGG + chr2_3:63288224-63288243 None:intergenic 35.0%
! TGTGCCATTGCTCAAGAATT+AGG + chr2_3:63285153-63285172 None:intergenic 40.0%
CTTCACTCACTTAAAGAGCA+TGG + chr2_3:63285291-63285310 None:intergenic 40.0%
CATGCTCTTTAAGTGAGTGA+AGG - chr2_3:63285289-63285308 Msa0305550:three_prime_UTR 40.0%
GAACACAAAAGGAATGACCA+AGG - chr2_3:63285345-63285364 Msa0305550:three_prime_UTR 40.0%
TGGAAATTAGGAAAGAGGCT+TGG + chr2_3:63285421-63285440 None:intergenic 40.0%
! GACTCTGGAAATTAGGAAAG+AGG + chr2_3:63285426-63285445 None:intergenic 40.0%
GACTTAAACCCACCAAAAAG+AGG + chr2_3:63285779-63285798 None:intergenic 40.0%
CATTAATGAGCAGGTAACTG+TGG + chr2_3:63285903-63285922 None:intergenic 40.0%
AGATAGGAGCCGAGTTTATA+GGG - chr2_3:63286103-63286122 Msa0305550:three_prime_UTR 40.0%
!!! CTTTTGGTTCACAATAGCAG+TGG + chr2_3:63286157-63286176 None:intergenic 40.0%
CAAAAGAAATGGTGACTGCA+GGG + chr2_3:63286281-63286300 None:intergenic 40.0%
TTGCAGGTGCCTATGTATAT+TGG - chr2_3:63286476-63286495 Msa0305550:CDS 40.0%
! TTTCACCCTTTTATGAGTGG+TGG - chr2_3:63286548-63286567 Msa0305550:CDS 40.0%
TGACTCCACCACTCATAAAA+GGG + chr2_3:63286556-63286575 None:intergenic 40.0%
AAAAGCTTGACCATATCCTC+CGG + chr2_3:63286588-63286607 None:intergenic 40.0%
AATACTCACAGCTCTTGTGT+CGG + chr2_3:63286666-63286685 None:intergenic 40.0%
TTAATTGCTCTTCACGCGAA+GGG + chr2_3:63286826-63286845 None:intergenic 40.0%
GAGAGTGATAAGTGAGAGTT+AGG + chr2_3:63286956-63286975 None:intergenic 40.0%
TTCATTGACGCTCCTGTTAT+TGG + chr2_3:63287052-63287071 None:intergenic 40.0%
ATGAACCCAGTAAGAACTCT+AGG - chr2_3:63287067-63287086 Msa0305550:CDS 40.0%
AGCTAAGAGATAGACCCATA+TGG + chr2_3:63287122-63287141 None:intergenic 40.0%
ATGATGATTGATGAGCATGG+GGG + chr2_3:63287272-63287291 None:intergenic 40.0%
! TGCACATGGCCAAATGATTT+TGG + chr2_3:63287376-63287395 None:intergenic 40.0%
! AGCACTCAATTTTGCTTGCT+TGG + chr2_3:63287463-63287482 None:intergenic 40.0%
GTTGAACTTACGTCTTTGTG+GGG + chr2_3:63287716-63287735 None:intergenic 40.0%
GAGTTGAACTTACGTCTTTG+TGG + chr2_3:63287718-63287737 None:intergenic 40.0%
GAGAAAAAGGTAGCAGCATG+TGG + chr2_3:63285487-63285506 None:intergenic 45.0%
! ACTCCTGGTGTACCTCTTTT+TGG - chr2_3:63285764-63285783 Msa0305550:intron 45.0%
!! CTGGTGTACCTCTTTTTGGT+GGG - chr2_3:63285768-63285787 Msa0305550:intron 45.0%
GGGTTTAAGTCTGAGAGAAG+CGG - chr2_3:63285788-63285807 Msa0305550:intron 45.0%
GGTTTAAGTCTGAGAGAAGC+GGG - chr2_3:63285789-63285808 Msa0305550:intron 45.0%
!! AATGCTTCGGTGTCCAAGAA+TGG - chr2_3:63285855-63285874 Msa0305550:intron 45.0%
!!! TTTTTTGCAAGAGGCACAGG+AGG + chr2_3:63285935-63285954 None:intergenic 45.0%
TCCTGTGCCTCTTGCAAAAA+AGG - chr2_3:63285934-63285953 Msa0305550:intron 45.0%
!!! ACCTTTTTTGCAAGAGGCAC+AGG + chr2_3:63285938-63285957 None:intergenic 45.0%
CAGATAGGAGCCGAGTTTAT+AGG - chr2_3:63286102-63286121 Msa0305550:three_prime_UTR 45.0%
GAACCAAAAGACACAAGGCT+CGG - chr2_3:63286167-63286186 Msa0305550:three_prime_UTR 45.0%
ATGATCATCATCCTCTCCAC+CGG - chr2_3:63286231-63286250 Msa0305550:three_prime_UTR 45.0%
GCAAAAGAAATGGTGACTGC+AGG + chr2_3:63286282-63286301 None:intergenic 45.0%
CTTTGGTTACATGCTTCCAC+GGG + chr2_3:63286320-63286339 None:intergenic 45.0%
GCTTTGGTTACATGCTTCCA+CGG + chr2_3:63286321-63286340 None:intergenic 45.0%
TGTGCTGCATTTGCTCTCAA+AGG - chr2_3:63286521-63286540 Msa0305550:CDS 45.0%
GTGCTGCATTTGCTCTCAAA+GGG - chr2_3:63286522-63286541 Msa0305550:CDS 45.0%
TGCTGCATTTGCTCTCAAAG+GGG - chr2_3:63286523-63286542 Msa0305550:CDS 45.0%
GTGACTCCACCACTCATAAA+AGG + chr2_3:63286557-63286576 None:intergenic 45.0%
AAGGAAGATGAAGCCGACAT+AGG - chr2_3:63286750-63286769 Msa0305550:intron 45.0%
AGGAAGATGAAGCCGACATA+GGG - chr2_3:63286751-63286770 Msa0305550:intron 45.0%
TAATTGCTCTTCACGCGAAG+GGG + chr2_3:63286825-63286844 None:intergenic 45.0%
GTTAATTGCTCTTCACGCGA+AGG + chr2_3:63286827-63286846 None:intergenic 45.0%
ATGTAGGTAGGAGAACTAGC+AGG - chr2_3:63286873-63286892 Msa0305550:intron 45.0%
AGAACTAGCAGGAATTGCAG+TGG - chr2_3:63286884-63286903 Msa0305550:intron 45.0%
GAACTAGCAGGAATTGCAGT+GGG - chr2_3:63286885-63286904 Msa0305550:intron 45.0%
TTCATGACAAGTGGGGATAG+GGG + chr2_3:63286999-63287018 None:intergenic 45.0%
GTTCATGACAAGTGGGGATA+GGG + chr2_3:63287000-63287019 None:intergenic 45.0%
TGTTCATGACAAGTGGGGAT+AGG + chr2_3:63287001-63287020 None:intergenic 45.0%
CTGGACCTAGAGTTCTTACT+GGG + chr2_3:63287075-63287094 None:intergenic 45.0%
!! CAATACTTGGAGCTCTAGGT+GGG - chr2_3:63287143-63287162 Msa0305550:CDS 45.0%
!! AATACTTGGAGCTCTAGGTG+GGG - chr2_3:63287144-63287163 Msa0305550:CDS 45.0%
AAGTTCAACTCTGCCAGTGT+TGG - chr2_3:63287727-63287746 Msa0305550:intron 45.0%
AGTTCAACTCTGCCAGTGTT+GGG - chr2_3:63287728-63287747 Msa0305550:intron 45.0%
GCAATCTGCTATGATCCTTG+AGG - chr2_3:63287763-63287782 Msa0305550:intron 45.0%
! TTGATGGGCAATCTGCTTTG+AGG - chr2_3:63287895-63287914 Msa0305550:CDS 45.0%
! TTTGAGGCATGATGCCTGTT+TGG - chr2_3:63287911-63287930 Msa0305550:CDS 45.0%
TCAAATCAGCCCATCCAAAC+AGG + chr2_3:63287928-63287947 None:intergenic 45.0%
AGGGAAATGCCTTGAGATTG+AGG - chr2_3:63288245-63288264 Msa0305550:five_prime_UTR 45.0%
GAAATGCCTTGAGATTGAGG+AGG - chr2_3:63288248-63288267 Msa0305550:five_prime_UTR 45.0%
TCTCATCCTCCTCAATCTCA+AGG + chr2_3:63288257-63288276 None:intergenic 45.0%
TCGGAAAACAGAAAGAGCGA+AGG - chr2_3:63288301-63288320 Msa0305550:five_prime_UTR 45.0%
AACACCGACGATATCAGCAA+TGG - chr2_3:63288334-63288353 Msa0305550:five_prime_UTR 45.0%
AATTTGTCTGCGCCTAACCA+TGG - chr2_3:63288545-63288564 Msa0305550:five_prime_UTR 45.0%
ATTTGTCTGCGCCTAACCAT+GGG - chr2_3:63288546-63288565 Msa0305550:five_prime_UTR 45.0%
AAGATGAAGACACAGACCCA+TGG + chr2_3:63288565-63288584 None:intergenic 45.0%
! GGCTTGGTTCACACTACACT+AGG + chr2_3:63285405-63285424 None:intergenic 50.0%
AGGGGACTCTTACTAGACTC+TGG + chr2_3:63285441-63285460 None:intergenic 50.0%
TAGCAGCATGTGGAAGTGGA+CGG + chr2_3:63285477-63285496 None:intergenic 50.0%
AAGGTAGCAGCATGTGGAAG+TGG + chr2_3:63285481-63285500 None:intergenic 50.0%
! CCACCAAAAAGAGGTACACC+AGG + chr2_3:63285770-63285789 None:intergenic 50.0%
! CCTGGTGTACCTCTTTTTGG+TGG - chr2_3:63285767-63285786 Msa0305550:intron 50.0%
AAGTCTGAGAGAAGCGGGAA+TGG - chr2_3:63285794-63285813 Msa0305550:intron 50.0%
TCGGCTCCTATCTGTAACAC+AGG + chr2_3:63286096-63286115 None:intergenic 50.0%
!! TCTCCGAGCCTTGTGTCTTT+TGG + chr2_3:63286173-63286192 None:intergenic 50.0%
AAGGCTCGGAGACTCTGATT+GGG - chr2_3:63286181-63286200 Msa0305550:three_prime_UTR 50.0%
ATTGGGTGTGCTACCTCTAC+TGG - chr2_3:63286198-63286217 Msa0305550:three_prime_UTR 50.0%
!! TGCTACCTCTACTGGTCTTG+CGG - chr2_3:63286206-63286225 Msa0305550:three_prime_UTR 50.0%
CATAACCGCAAGACCAGTAG+AGG + chr2_3:63286214-63286233 None:intergenic 50.0%
ATGAGCACCAGAGATGTGAC+CGG + chr2_3:63286253-63286272 None:intergenic 50.0%
ACCATATCCTCCGGAAGGAA+CGG + chr2_3:63286579-63286598 None:intergenic 50.0%
AATGTTCGTTGTCACGGCAG+TGG - chr2_3:63286637-63286656 Msa0305550:CDS 50.0%
GGAAGATGAAGCCGACATAG+GGG - chr2_3:63286752-63286771 Msa0305550:intron 50.0%
TCATGACAAGTGGGGATAGG+GGG + chr2_3:63286998-63287017 None:intergenic 50.0%
GCTGGACCTAGAGTTCTTAC+TGG + chr2_3:63287076-63287095 None:intergenic 50.0%
GCTCCAATACTTGGAGCTCT+AGG - chr2_3:63287139-63287158 Msa0305550:CDS 50.0%
CCACCTAGAGCTCCAAGTAT+TGG + chr2_3:63287145-63287164 None:intergenic 50.0%
!! CCAATACTTGGAGCTCTAGG+TGG - chr2_3:63287142-63287161 Msa0305550:CDS 50.0%
! AAGTTTAGGTGAGTTGGGCC+CGG + chr2_3:63287810-63287829 None:intergenic 50.0%
AGGCATGATGCCTGTTTGGA+TGG - chr2_3:63287915-63287934 Msa0305550:CDS 50.0%
GGCATGATGCCTGTTTGGAT+GGG - chr2_3:63287916-63287935 Msa0305550:CDS 50.0%
!! GATGCGGAGACGTTTTGGAT+CGG - chr2_3:63288282-63288301 Msa0305550:five_prime_UTR 50.0%
GACGCCATTGCTGATATCGT+CGG + chr2_3:63288341-63288360 None:intergenic 50.0%
ATCAGCAATGGCGTCCTTGA+AGG - chr2_3:63288346-63288365 Msa0305550:five_prime_UTR 50.0%
AACGACGGATCCGATGGAAA+TGG - chr2_3:63288436-63288455 Msa0305550:five_prime_UTR 50.0%
GAAGACACAGACCCATGGTT+AGG + chr2_3:63288560-63288579 None:intergenic 50.0%
AGCATGTGGAAGTGGACGGT+GGG + chr2_3:63285473-63285492 None:intergenic 55.0%
!! CCTGGTGTTGCTGGTGTTGA+TGG + chr2_3:63285743-63285762 None:intergenic 55.0%
CCATCAACACCAGCAACACC+AGG - chr2_3:63285740-63285759 Msa0305550:intron 55.0%
AGGGGGTGGCATTAATGAGC+AGG + chr2_3:63285912-63285931 None:intergenic 55.0%
! TTGCAAGAGGCACAGGAGGA+GGG + chr2_3:63285931-63285950 None:intergenic 55.0%
! TTTGCAAGAGGCACAGGAGG+AGG + chr2_3:63285932-63285951 None:intergenic 55.0%
CAAGGCTCGGAGACTCTGAT+TGG - chr2_3:63286180-63286199 Msa0305550:three_prime_UTR 55.0%
! CTGCATTGAAGCACTTCCCG+TGG - chr2_3:63286301-63286320 Msa0305550:three_prime_UTR 55.0%
ACCGTTCCTTCCGGAGGATA+TGG - chr2_3:63286575-63286594 Msa0305550:CDS 55.0%
GCTTGACCATATCCTCCGGA+AGG + chr2_3:63286584-63286603 None:intergenic 55.0%
ACTCACAGCTCTTGTGTCGG+TGG + chr2_3:63286663-63286682 None:intergenic 55.0%
AAGTGGGGATAGGGGGAAGA+AGG + chr2_3:63286991-63287010 None:intergenic 55.0%
! ATGATGGTGCACTGCGTTGC+AGG - chr2_3:63287026-63287045 Msa0305550:intron 55.0%
CTGCGTTGCAGGCCAATAAC+AGG - chr2_3:63287037-63287056 Msa0305550:intron 55.0%
TACCCTGACCTGGTAAGTCC+CGG - chr2_3:63287788-63287807 Msa0305550:CDS 55.0%
! AGTTTAGGTGAGTTGGGCCC+GGG + chr2_3:63287809-63287828 None:intergenic 55.0%
GGAGGATGAGAGCGATGATG+CGG - chr2_3:63288266-63288285 Msa0305550:five_prime_UTR 55.0%
! GCGATGATGCGGAGACGTTT+TGG - chr2_3:63288277-63288296 Msa0305550:five_prime_UTR 55.0%
CTCTTCCGGTGCTTCCTTCA+AGG + chr2_3:63288363-63288382 None:intergenic 55.0%
!! TGAAGGAAGCACCGGAAGAG+CGG - chr2_3:63288363-63288382 Msa0305550:five_prime_UTR 55.0%
! CTCGGCTATTTCCGCTCTTC+CGG + chr2_3:63288377-63288396 None:intergenic 55.0%
GCGGAAATAGCCGAGGTGAT+CGG - chr2_3:63288382-63288401 Msa0305550:five_prime_UTR 55.0%
GAAATAGCCGAGGTGATCGG+CGG - chr2_3:63288385-63288404 Msa0305550:five_prime_UTR 55.0%
AAAGAAGCCGCCGATCACCT+CGG + chr2_3:63288395-63288414 None:intergenic 55.0%
GTGATCGGCGGCTTCTTTAG+CGG - chr2_3:63288397-63288416 Msa0305550:five_prime_UTR 55.0%
GAGAGAAACGACGGATCCGA+TGG - chr2_3:63288430-63288449 Msa0305550:five_prime_UTR 55.0%
ATCCGATGGAAATGGCGTGG+CGG - chr2_3:63288444-63288463 Msa0305550:five_prime_UTR 55.0%
!! GACGGTGGGACAGTTCTGAG+GGG + chr2_3:63285459-63285478 None:intergenic 60.0%
! GGACGGTGGGACAGTTCTGA+GGG + chr2_3:63285460-63285479 None:intergenic 60.0%
TGGACGGTGGGACAGTTCTG+AGG + chr2_3:63285461-63285480 None:intergenic 60.0%
CAGCATGTGGAAGTGGACGG+TGG + chr2_3:63285474-63285493 None:intergenic 60.0%
!! AGAGGTACACCAGGAGTGCC+TGG + chr2_3:63285761-63285780 None:intergenic 60.0%
! TGCAAGAGGCACAGGAGGAG+GGG + chr2_3:63285930-63285949 None:intergenic 60.0%
CAGAGATGTGACCGGTGGAG+AGG + chr2_3:63286245-63286264 None:intergenic 60.0%
CTCTCCACCGGTCACATCTC+TGG - chr2_3:63286243-63286262 Msa0305550:three_prime_UTR 60.0%
AGCACCAGAGATGTGACCGG+TGG + chr2_3:63286250-63286269 None:intergenic 60.0%
GGTGGAGTCACCGTTCCTTC+CGG - chr2_3:63286566-63286585 Msa0305550:CDS 60.0%
! CTGCCAGTGTTGGGACTCGT+TGG - chr2_3:63287737-63287756 Msa0305550:intron 60.0%
! CCAGTGTTGGGACTCGTTGG+CGG - chr2_3:63287740-63287759 Msa0305550:intron 60.0%
! CAGTGTTGGGACTCGTTGGC+GGG - chr2_3:63287741-63287760 Msa0305550:intron 60.0%
CCAGGTCAGGGTAAGCCTCA+AGG + chr2_3:63287781-63287800 None:intergenic 60.0%
CCTTGAGGCTTACCCTGACC+TGG - chr2_3:63287778-63287797 Msa0305550:CDS 60.0%
ACCCTGACCTGGTAAGTCCC+GGG - chr2_3:63287789-63287808 Msa0305550:CDS 60.0%
! GGCGTCCTTGAAGGAAGCAC+CGG - chr2_3:63288355-63288374 Msa0305550:five_prime_UTR 60.0%
CGGAAGAGCGGAAATAGCCG+AGG - chr2_3:63288375-63288394 Msa0305550:five_prime_UTR 60.0%
TCCGTCGTTTCTCTCCTCGC+CGG + chr2_3:63288425-63288444 None:intergenic 60.0%
CGGATCCGATGGAAATGGCG+TGG - chr2_3:63288441-63288460 Msa0305550:five_prime_UTR 60.0%
!! CCAGGAGTGCCTGGTGTTGC+TGG + chr2_3:63285752-63285771 None:intergenic 65.0%
CCAGCAACACCAGGCACTCC+TGG - chr2_3:63285749-63285768 Msa0305550:intron 65.0%
! GCAAGAGGCACAGGAGGAGG+GGG + chr2_3:63285929-63285948 None:intergenic 65.0%
GGAGTCACCGTTCCTTCCGG+AGG - chr2_3:63286569-63286588 Msa0305550:CDS 65.0%
!! CTTGGAGCTCTAGGTGGGGC+AGG - chr2_3:63287148-63287167 Msa0305550:CDS 65.0%
CCGCCAACGAGTCCCAACAC+TGG + chr2_3:63287743-63287762 None:intergenic 65.0%
GCCCGGGACTTACCAGGTCA+GGG + chr2_3:63287793-63287812 None:intergenic 65.0%
AGTTGGGCCCGGGACTTACC+AGG + chr2_3:63287799-63287818 None:intergenic 65.0%
GGCGGCTTCTTTAGCGGAGC+CGG - chr2_3:63288403-63288422 Msa0305550:five_prime_UTR 65.0%
CTTCTTTAGCGGAGCCGGCG+AGG - chr2_3:63288408-63288427 Msa0305550:five_prime_UTR 65.0%
GCCGGCGAGGAGAGAAACGA+CGG - chr2_3:63288421-63288440 Msa0305550:five_prime_UTR 65.0%
CGCCGCCACGCCATTTCCAT+CGG + chr2_3:63288449-63288468 None:intergenic 65.0%
CGATGGAAATGGCGTGGCGG+CGG - chr2_3:63288447-63288466 Msa0305550:five_prime_UTR 65.0%
! AGAGGCACAGGAGGAGGGGG+TGG + chr2_3:63285926-63285945 None:intergenic 70.0%
GGCCCGGGACTTACCAGGTC+AGG + chr2_3:63287794-63287813 None:intergenic 70.0%
TGGAAATGGCGTGGCGGCGG+AGG - chr2_3:63288450-63288469 Msa0305550:five_prime_UTR 70.0%
ATGGCGTGGCGGCGGAGGAA+AGG - chr2_3:63288455-63288474 Msa0305550:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 63285117 63288611 63285117 ID=Msa0305550;Name=Msa0305550
chr2_3 mRNA 63285117 63288611 63285117 ID=Msa0305550-mRNA-1;Parent=Msa0305550;Name=Msa0305550-mRNA-1;_AED=0.08;_eAED=0.08;_QI=596|1|1|1|0.8|0.66|6|920|310
chr2_3 exon 63285117 63285506 63285117 ID=Msa0305550-mRNA-1:exon:21137;Parent=Msa0305550-mRNA-1
chr2_3 exon 63285943 63286680 63285943 ID=Msa0305550-mRNA-1:exon:21136;Parent=Msa0305550-mRNA-1
chr2_3 exon 63286788 63286849 63286788 ID=Msa0305550-mRNA-1:exon:21135;Parent=Msa0305550-mRNA-1
chr2_3 exon 63287052 63287246 63287052 ID=Msa0305550-mRNA-1:exon:21134;Parent=Msa0305550-mRNA-1
chr2_3 exon 63287399 63287623 63287399 ID=Msa0305550-mRNA-1:exon:21133;Parent=Msa0305550-mRNA-1
chr2_3 exon 63287773 63288611 63287773 ID=Msa0305550-mRNA-1:exon:21132;Parent=Msa0305550-mRNA-1
chr2_3 five_prime_UTR 63288016 63288611 63288016 ID=Msa0305550-mRNA-1:five_prime_utr;Parent=Msa0305550-mRNA-1
chr2_3 CDS 63287773 63288015 63287773 ID=Msa0305550-mRNA-1:cds;Parent=Msa0305550-mRNA-1
chr2_3 CDS 63287399 63287623 63287399 ID=Msa0305550-mRNA-1:cds;Parent=Msa0305550-mRNA-1
chr2_3 CDS 63287052 63287246 63287052 ID=Msa0305550-mRNA-1:cds;Parent=Msa0305550-mRNA-1
chr2_3 CDS 63286788 63286849 63286788 ID=Msa0305550-mRNA-1:cds;Parent=Msa0305550-mRNA-1
chr2_3 CDS 63286473 63286680 63286473 ID=Msa0305550-mRNA-1:cds;Parent=Msa0305550-mRNA-1
chr2_3 three_prime_UTR 63285943 63286472 63285943 ID=Msa0305550-mRNA-1:three_prime_utr;Parent=Msa0305550-mRNA-1
chr2_3 three_prime_UTR 63285117 63285506 63285117 ID=Msa0305550-mRNA-1:three_prime_utr;Parent=Msa0305550-mRNA-1
Gene Sequence

>Msa0305550

ATGGAACATATTAATAATGAGGAAATTCCATCAACACCAGCAACACCAGGCACTCCTGGTGTACCTCTTTTTGGTGGGTTTAAGTCTGAGAGAAGCGGGAATGGTAATAATAAATATAAGAAGTCCCTACTCAAGAATTGTAAATGCTTCGGTGTCCAAGAATGGAACTTAGAAGATGGAGCTCTACCCACAGTTACCTGCTCATTAATGCCACCCCCTCCTCCTGTGCCTCTTGCAAAAAAGATAGGAGCCGAGTTTATAGGGACATTGATTCTGATGTTTGCTGGGACAGCCACTGCTATTGTGAACCAAAAGACACAAGGCTCGGAGACTCTGATTGGGTGTGCTACCTCTACTGGTCTTGCGGTTATGATCATCATCCTCTCCACCGGTCACATCTCTGGTGCTCATCTCAACCCTGCAGTCACCATTTCTTTTGCTGCATTGAAGCACTTCCCGTGGAAGCATGTGCCTATGTATATTGGTGCACAAGTTTTGGCATCAATATGTGCTGCATTTGCTCTCAAAGGGGTTTTTCACCCTTTTATGAGTGGTGGAGTCACCGTTCCTTCCGGAGGATATGGTCAAGCTTTTGCTTTAGAATTCATTATCAGCTTTAATCTAATGTTCGTTGTCACGGCAGTGGCCACCGACACAAGAGCTGTAGGAGAACTAGCAGGAATTGCAGTGGGAGCAACTGTGATGCTCAATATACTAATAGCAGGGCCAATAACAGGAGCGTCAATGAACCCAGTAAGAACTCTAGGTCCAGCTATAGCTGCAAATAACTACAAAGCCATATGGGTCTATCTCTTAGCTCCAATACTTGGAGCTCTAGGTGGGGCAGGAACTTATACTGCAGTCAAGCTTCCCGAAGAAGATGATAATGCAAAGACTAATGCTTCTTCAAATCACCCCAGCTTTAGAAGATGA
Protein sequence

>Msa0305550

MEHINNEEIPSTPATPGTPGVPLFGGFKSERSGNGNNKYKKSLLKNCKCFGVQEWNLEDGALPTVTCSLMPPPPPVPLAKKIGAEFIGTLILMFAGTATAIVNQKTQGSETLIGCATSTGLAVMIIILSTGHISGAHLNPAVTISFAALKHFPWKHVPMYIGAQVLASICAAFALKGVFHPFMSGGVTVPSGGYGQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAGPITGASMNPVRTLGPAIAANNYKAIWVYLLAPILGALGGAGTYTAVKLPEEDDNAKTNASSNHPSFRR*