AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0259670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0259670 MtrunA17_Chr2g0333771 98.387 310 5 0 1 310 1 310 0.0 619
Msa0259670 MtrunA17_Chr4g0001221 83.548 310 43 3 4 310 1 305 0.0 506
Msa0259670 MtrunA17_Chr4g0001111 83.784 222 28 3 4 222 1 217 1.40e-128 365
Msa0259670 MtrunA17_Chr1g0201331 58.224 304 107 4 11 310 13 300 3.45e-110 322
Msa0259670 MtrunA17_Chr4g0001161 83.571 140 23 0 93 232 1 140 1.29e-77 233
Msa0259670 MtrunA17_Chr8g0379901 48.473 262 130 2 45 303 4 263 1.18e-76 235
Msa0259670 MtrunA17_Chr8g0379911 51.542 227 105 2 80 303 38 262 1.11e-74 230
Msa0259670 MtrunA17_Chr2g0284631 47.710 262 133 2 53 310 64 325 4.71e-74 230
Msa0259670 MtrunA17_Chr2g0284591 49.206 252 119 3 56 303 14 260 6.24e-73 226
Msa0259670 MtrunA17_Chr2g0284611 49.206 252 119 3 56 303 16 262 2.48e-72 224
Msa0259670 MtrunA17_Chr7g0217331 46.121 232 122 1 75 306 32 260 1.50e-71 222
Msa0259670 MtrunA17_Chr2g0284581 51.584 221 102 2 76 293 34 252 1.70e-69 217
Msa0259670 MtrunA17_Chr2g0284601 50.485 206 97 2 91 293 1 204 1.31e-61 195
Msa0259670 MtrunA17_Chr4g0001151 77.863 131 14 1 93 223 1 116 2.47e-60 195
Msa0259670 MtrunA17_Chr4g0044201 42.045 264 128 5 56 310 8 255 4.12e-60 192
Msa0259670 MtrunA17_Chr5g0435051 45.495 222 116 3 76 293 36 256 1.99e-57 186
Msa0259670 MtrunA17_Chr3g0079421 47.685 216 109 3 76 289 22 235 2.37e-55 180
Msa0259670 MtrunA17_Chr5g0426131 43.779 217 116 2 80 292 37 251 4.14e-55 180
Msa0259670 MtrunA17_Chr1g0184541 40.175 229 137 0 78 306 60 288 4.95e-51 170
Msa0259670 MtrunA17_Chr4g0001121 82.022 89 16 0 222 310 10 98 2.14e-43 144
Msa0259670 MtrunA17_Chr2g0331261 39.286 224 116 8 84 293 24 241 2.47e-32 120
Msa0259670 MtrunA17_Chr1g0146161 37.073 205 109 7 85 277 24 220 4.79e-29 111
Msa0259670 MtrunA17_Chr8g0340161 35.749 207 115 4 84 277 23 224 3.10e-27 106
Msa0259670 MtrunA17_Chr1g0188771 34.404 218 135 3 84 293 25 242 4.27e-26 103
Msa0259670 MtrunA17_Chr1g0146171 36.098 205 111 8 85 277 24 220 5.33e-25 100
Msa0259670 MtrunA17_Chr6g0478191 32.287 223 143 3 84 298 25 247 2.00e-24 99.4
Msa0259670 MtrunA17_Chr2g0326621 31.120 241 132 7 71 278 22 261 1.86e-23 97.4
Msa0259670 MtrunA17_Chr5g0399341 34.783 207 116 4 84 277 23 223 3.89e-23 95.9
Msa0259670 MtrunA17_Chr3g0145671 30.453 243 142 7 73 288 19 261 6.69e-23 95.9
Msa0259670 MtrunA17_Chr4g0030861 33.168 202 127 4 85 278 26 227 1.28e-21 91.7
Msa0259670 MtrunA17_Chr1g0212671 29.075 227 153 3 84 302 25 251 5.66e-21 90.1
Msa0259670 MtrunA17_Chr7g0264111 28.452 239 140 7 71 278 21 259 1.10e-18 84.3
Msa0259670 MtrunA17_Chr7g0264961 33.010 206 121 6 84 277 25 225 1.47e-18 83.2
Msa0259670 MtrunA17_Chr3g0113211 29.508 244 144 8 73 288 37 280 2.84e-18 83.2
Msa0259670 MtrunA17_Chr8g0386961 29.487 234 137 8 73 278 34 267 9.94e-18 81.6
Msa0259670 MtrunA17_Chr4g0006001 29.717 212 131 7 84 278 60 270 1.38e-16 78.6
Msa0259670 MtrunA17_Chr5g0397391 30.189 212 131 6 84 278 56 267 2.02e-16 78.2
Msa0259670 MtrunA17_Chr4g0029071 27.197 239 141 7 73 278 21 259 2.35e-16 77.8
Msa0259670 MtrunA17_Chr7g0222791 27.753 227 156 4 84 302 25 251 9.76e-16 75.5
Msa0259670 MtrunA17_Chr5g0436701 28.692 237 135 8 73 278 37 270 2.74e-15 74.7
Msa0259670 MtrunA17_Chr1g0199731 26.778 239 142 7 73 278 21 259 4.26e-15 74.3
Msa0259670 MtrunA17_Chr4g0020781 29.648 199 130 5 111 303 15 209 1.58e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0259670 AT1G80760.1 77.564 312 58 4 4 310 1 305 6.75e-167 466
Msa0259670 AT4G10380.1 67.416 267 75 3 46 310 48 304 5.13e-119 344
Msa0259670 AT5G37820.1 46.484 256 129 4 52 303 16 267 8.23e-72 224
Msa0259670 AT5G37820.2 46.484 256 129 4 52 303 52 303 1.24e-71 224
Msa0259670 AT4G19030.1 43.686 293 151 5 29 309 2 292 1.56e-70 221
Msa0259670 AT5G37810.1 46.964 247 125 3 59 303 25 267 5.87e-70 219
Msa0259670 AT4G18910.1 41.472 299 146 4 29 309 2 289 2.88e-69 218
Msa0259670 AT1G31885.1 45.600 250 128 2 68 309 31 280 2.60e-66 211
Msa0259670 AT1G31885.2 46.903 226 112 2 92 309 1 226 2.95e-61 196
Msa0259670 AT2G34390.1 40.217 276 148 5 46 310 13 282 1.84e-54 179
Msa0259670 AT2G34390.2 42.083 240 123 4 81 310 7 240 1.53e-51 170
Msa0259670 AT4G19030.3 44.000 225 112 5 29 241 2 224 2.61e-49 164
Msa0259670 AT4G18910.2 42.241 232 105 4 29 242 2 222 2.05e-48 161
Msa0259670 AT3G06100.1 38.208 212 130 1 80 290 46 257 1.50e-43 150
Msa0259670 AT3G06100.2 38.389 211 129 1 84 293 2 212 6.14e-43 147
Msa0259670 AT4G19030.2 41.748 206 106 5 29 222 2 205 1.53e-38 136
Msa0259670 AT2G34390.3 38.942 208 113 4 46 242 13 217 9.26e-35 126
Msa0259670 AT3G16240.1 37.198 207 112 5 84 277 23 224 1.16e-29 113
Msa0259670 AT2G34390.4 37.368 190 105 4 46 224 13 199 8.82e-28 108
Msa0259670 AT2G25810.1 35.885 209 116 7 80 277 19 220 3.40e-26 104
Msa0259670 AT5G47450.1 34.783 230 124 7 84 292 23 247 3.77e-26 104
Msa0259670 AT2G29870.1 46.957 115 58 1 196 310 22 133 1.12e-25 100
Msa0259670 AT4G17340.1 33.816 207 119 4 84 277 23 224 2.63e-24 99.4
Msa0259670 AT3G26520.1 34.783 207 117 6 84 277 26 227 7.96e-23 95.5
Msa0259670 AT2G36830.1 34.783 207 117 6 84 277 25 226 8.65e-23 95.5
Msa0259670 AT4G35100.1 30.864 243 141 7 73 288 20 262 4.12e-22 94.0
Msa0259670 AT4G35100.2 30.864 243 141 7 73 288 20 262 4.12e-22 94.0
Msa0259670 AT2G16850.1 30.328 244 141 7 73 288 18 260 4.74e-22 94.0
Msa0259670 AT5G60660.1 28.689 244 141 7 71 282 21 263 1.73e-20 89.7
Msa0259670 AT3G06100.3 34.532 139 90 1 156 293 41 179 7.77e-20 85.9
Msa0259670 AT3G54820.1 30.833 240 133 7 71 278 20 258 1.93e-19 87.0
Msa0259670 AT1G73190.1 30.493 223 142 4 84 293 28 250 1.43e-18 84.0
Msa0259670 AT4G01470.1 30.275 218 134 5 84 288 25 237 2.32e-18 83.2
Msa0259670 AT1G17810.1 29.956 227 142 6 84 295 28 252 8.86e-18 82.0
Msa0259670 AT2G37170.1 27.308 260 145 8 51 278 10 257 1.36e-17 81.6
Msa0259670 AT3G53420.1 27.917 240 140 7 71 278 21 259 1.62e-17 81.6
Msa0259670 AT3G53420.2 27.917 240 140 7 71 278 21 259 1.62e-17 81.6
Msa0259670 AT2G37170.2 27.308 260 145 8 51 278 53 300 3.02e-17 81.3
Msa0259670 AT2G45960.1 31.915 235 129 9 73 278 34 266 8.17e-17 79.3
Msa0259670 AT2G37180.1 26.923 260 146 8 51 278 10 257 1.09e-16 79.0
Msa0259670 AT4G23400.1 30.189 212 130 6 84 278 57 267 1.32e-16 79.0
Msa0259670 AT4G00430.1 29.487 234 136 8 73 278 35 267 1.45e-16 79.0
Msa0259670 AT1G31885.3 41.758 91 53 0 66 156 29 119 2.05e-16 75.1
Msa0259670 AT1G01620.1 30.660 212 129 6 84 278 56 266 2.45e-16 78.2
Msa0259670 AT2G39010.1 28.333 240 137 7 73 278 20 258 2.56e-16 78.2
Msa0259670 AT3G61430.2 31.455 213 126 7 84 278 56 266 3.38e-16 77.8
Msa0259670 AT3G61430.1 31.455 213 126 7 84 278 56 266 3.38e-16 77.8
Msa0259670 AT3G47440.1 27.586 232 151 5 84 303 27 253 2.35e-15 74.7
Msa0259670 AT1G01620.2 32.402 179 106 5 114 278 17 194 1.41e-14 72.0
Msa0259670 AT2G45960.3 30.594 219 126 7 73 266 34 251 2.09e-14 72.8
Msa0259670 AT2G45960.2 31.818 220 122 8 73 266 34 251 2.58e-14 72.0

Find 160 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAATCATGATTAATTAAT+TGG 0.122870 2_2:+56935206 Msa0259670:five_prime_UTR
TGCACATGGCCAAATGATTT+TGG 0.183955 2_2:-56937356 None:intergenic
TTCATTGACGCTCCTGTTAT+TGG 0.213047 2_2:-56936990 None:intergenic
CATCTTCTAAGTTCCATTCT+TGG 0.216409 2_2:-56935809 None:intergenic
GTGACTCCACCACTCATAAA+AGG 0.246044 2_2:-56936495 None:intergenic
AGTAACTAATTGTTGTTTAC+AGG 0.248708 2_2:-56935134 None:intergenic
CTTACTAGACTCTGGAAATT+AGG 0.255989 2_2:-56935371 None:intergenic
CTACAAACTTGCAGAAATTA+AGG 0.267685 2_2:+56935262 Msa0259670:five_prime_UTR
TCTCCGAGCCTTGTGTCTTT+TGG 0.294166 2_2:-56936111 None:intergenic
CTTTGCATTATCATCTTCTT+CGG 0.298809 2_2:-56937135 None:intergenic
CAGATAGGAGCCGAGTTTAT+AGG 0.306620 2_2:+56936043 Msa0259670:intron
GAAACTGATATATGAGAAAA+AGG 0.310726 2_2:-56935438 None:intergenic
TGTGCCATTGCTCAAGAATT+AGG 0.311160 2_2:-56935091 None:intergenic
CCAGGAGTGCCTGGTGTTGC+TGG 0.322227 2_2:-56935690 None:intergenic
CCTGGTGTTGCTGGTGTTGA+TGG 0.322832 2_2:-56935681 None:intergenic
CACTAGGCATCAATTATATA+CGG 0.333235 2_2:-56935327 None:intergenic
GTATATTGGTGCACAAGTTT+TGG 0.339591 2_2:+56936431 Msa0259670:CDS
TTTACCATTGACTGTAGAAA+TGG 0.347231 2_2:+56935635 Msa0259670:exon
ATAAGTTATAACACCCTGTT+TGG 0.350832 2_2:+56937902 Msa0259670:three_prime_UTR
AGATAGGAGCCGAGTTTATA+GGG 0.358525 2_2:+56936044 Msa0259670:intron
TTTGAGGCATGATGCCTGTT+TGG 0.374318 2_2:+56937851 Msa0259670:three_prime_UTR
TTTCAAAGTAAAGTTATTGA+TGG 0.375157 2_2:+56937819 Msa0259670:three_prime_UTR
GCTGGACCTAGAGTTCTTAC+TGG 0.377381 2_2:-56937014 None:intergenic
CTGCGTTGCAGGCCAATAAC+AGG 0.388557 2_2:+56936978 Msa0259670:intron
CTCTCCACCGGCCACATCTC+TGG 0.392505 2_2:+56936184 Msa0259670:CDS
ACCGTTCCTTCCGGAGGATA+TGG 0.393092 2_2:+56936516 Msa0259670:CDS
GAGTTGAACTTACGTCTTTG+TGG 0.400319 2_2:-56937698 None:intergenic
ATTAGAGATAATTAATTACT+TGG 0.401264 2_2:-56937553 None:intergenic
TAGTTATTTGCAGCTATAGC+TGG 0.401428 2_2:-56937032 None:intergenic
ATTGGGTGTGCTACCTCTAC+TGG 0.401500 2_2:+56936139 Msa0259670:CDS
CAAATAACTACAAAGCCATA+TGG 0.404031 2_2:+56937045 Msa0259670:CDS
ACATTGATTCTGATGTTTGC+TGG 0.409739 2_2:+56936067 Msa0259670:CDS
AATTGAGTGCTCTTTCAGTT+TGG 0.411453 2_2:+56937455 Msa0259670:three_prime_UTR
CAAGGCTCGGAGACTCTGAT+TGG 0.413496 2_2:+56936121 Msa0259670:CDS
TATTGTTGCTATTGTATGTC+GGG 0.414071 2_2:-56935555 None:intergenic
TTTGCATTATCATCTTCTTC+GGG 0.414167 2_2:-56937134 None:intergenic
TGCAAGAGGCACAGGAGGAG+GGG 0.422658 2_2:-56935868 None:intergenic
TTTCCATTCATCAATTTCCT+TGG 0.427402 2_2:-56935303 None:intergenic
CATTGATTCTGATGTTTGCT+GGG 0.434089 2_2:+56936068 Msa0259670:CDS
CTCAAGAATTGTAAATGCTT+CGG 0.434143 2_2:+56935783 Msa0259670:CDS
TGTTCCATTTCTACAGTCAA+TGG 0.435070 2_2:-56935639 None:intergenic
CCACCTAGAGCTCCAAGTAT+TGG 0.435294 2_2:-56937083 None:intergenic
GGTGGAGTCACCGTTCCTTC+CGG 0.437337 2_2:+56936507 Msa0259670:CDS
GTGCTGCATTTGCTCTCAAA+GGG 0.443470 2_2:+56936463 Msa0259670:CDS
AGGCATGATGCCTGTTTGGA+TGG 0.444544 2_2:+56937855 Msa0259670:three_prime_UTR
TTTGCAAGAGGCACAGGAGG+AGG 0.446500 2_2:-56935870 None:intergenic
TATCTCTTAGCTCCAATACT+TGG 0.455159 2_2:+56937071 Msa0259670:CDS
TAGCATGACTAGTATCACTA+TGG 0.456539 2_2:-56935487 None:intergenic
AGTTGATCATCTTCTAAAGC+TGG 0.459544 2_2:-56937180 None:intergenic
ATTAGCAACAGATAAAATAT+TGG 0.459980 2_2:+56937592 Msa0259670:three_prime_UTR
TCAAATCAGCCCATCCAAAC+AGG 0.460558 2_2:-56937865 None:intergenic
TTGCAGGTGCCTATGTATAT+TGG 0.461576 2_2:+56936417 Msa0259670:intron
AATACACTCTCATGATACTT+AGG 0.463385 2_2:+56935177 Msa0259670:five_prime_UTR
TTAATTATCTCTAATTACAT+AGG 0.466666 2_2:+56937561 Msa0259670:three_prime_UTR
AATTTACATTAGACAAGAAA+AGG 0.467550 2_2:-56935614 None:intergenic
CTTCAATGCAGCAAAAGAAA+TGG 0.468532 2_2:-56936230 None:intergenic
AGTTGAACTTACGTCTTTGT+GGG 0.469754 2_2:-56937697 None:intergenic
TTGATGGGCAATCTGCTTTG+AGG 0.471425 2_2:+56937835 Msa0259670:three_prime_UTR
ATGCTCAATATACTAATAGC+AGG 0.472157 2_2:+56936859 Msa0259670:CDS
TTGCAAGAGGCACAGGAGGA+GGG 0.478192 2_2:-56935869 None:intergenic
CAGTGTTGGGACTCGTTGGC+GGG 0.479451 2_2:+56937724 Msa0259670:three_prime_UTR
AGAGGTACACCAGGAGTGCC+TGG 0.482854 2_2:-56935699 None:intergenic
TAAGTTATAACACCCTGTTT+GGG 0.487316 2_2:+56937903 Msa0259670:three_prime_UTR
CCAGTGTTGGGACTCGTTGG+CGG 0.490261 2_2:+56937723 Msa0259670:three_prime_UTR
AAATAACTACAAAGCCATAT+GGG 0.491686 2_2:+56937046 Msa0259670:CDS
TTCAAAGTAAAGTTATTGAT+GGG 0.492195 2_2:+56937820 Msa0259670:three_prime_UTR
AATGCTTCGGTGTCCAAGAA+TGG 0.493128 2_2:+56935796 Msa0259670:CDS
AACTCTTGATTAGAAAGAGC+TGG 0.493233 2_2:+56937639 Msa0259670:three_prime_UTR
GAAATTAAGGAGAACACAAA+AGG 0.495825 2_2:+56935275 Msa0259670:five_prime_UTR
CTTGGAGCTCTAGGTGGGGC+AGG 0.496173 2_2:+56937089 Msa0259670:CDS
GCAAGAGGCACAGGAGGAGG+GGG 0.497170 2_2:-56935867 None:intergenic
GCATTTACAATTCTTGAGTA+GGG 0.497185 2_2:-56935778 None:intergenic
TACATGGTTAAAGTAAAAGA+TGG 0.501830 2_2:+56937518 Msa0259670:three_prime_UTR
GCAAAAGAAATGGTGACTGC+AGG 0.502542 2_2:-56936220 None:intergenic
TTCATAAGCTATCTTGGAGA+AGG 0.510833 2_2:-56937985 None:intergenic
AGTTCAACTCTGCCAGTGTT+GGG 0.512112 2_2:+56937711 Msa0259670:three_prime_UTR
CAAGAATGGAACTTAGAAGA+TGG 0.514046 2_2:+56935810 Msa0259670:CDS
CCAGCAACACCAGGCACTCC+TGG 0.515961 2_2:+56935690 Msa0259670:CDS
GTTGATCATCTTCTAAAGCT+GGG 0.517734 2_2:-56937179 None:intergenic
TCATCATAAAATGACACTAA+AGG 0.518324 2_2:+56937226 Msa0259670:three_prime_UTR
AATGAAACGAAGCAAATTAG+TGG 0.519273 2_2:-56935463 None:intergenic
AATGGAACATATTAATAATG+AGG 0.519868 2_2:+56935653 Msa0259670:exon
ATGAACCCAGTAAGAACTCT+AGG 0.522426 2_2:+56937008 Msa0259670:CDS
TGATCCTTGAGGCTTATTGA+AGG 0.522724 2_2:+56937757 Msa0259670:three_prime_UTR
TAATCTAATGTTCGTTGTCA+CGG 0.524885 2_2:+56936572 Msa0259670:CDS
AAGGTAGCAGCATGTGGAAG+TGG 0.525892 2_2:-56935419 None:intergenic
GACTCTGGAAATTAGGAAAG+AGG 0.528630 2_2:-56935364 None:intergenic
TTATCCTAATTCTTGAGCAA+TGG 0.528782 2_2:+56935087 Msa0259670:five_prime_UTR
TGACCAAGGAAATTGATGAA+TGG 0.530797 2_2:+56935300 Msa0259670:five_prime_UTR
TTATTGTTGCTATTGTATGT+CGG 0.531372 2_2:-56935556 None:intergenic
GGCATGATGCCTGTTTGGAT+GGG 0.531642 2_2:+56937856 Msa0259670:three_prime_UTR
GCTCCAATACTTGGAGCTCT+AGG 0.532633 2_2:+56937080 Msa0259670:CDS
AGCATTTACAATTCTTGAGT+AGG 0.536216 2_2:-56935779 None:intergenic
TGACTCCACCACTCATAAAA+GGG 0.537986 2_2:-56936494 None:intergenic
CTGCCAGTGTTGGGACTCGT+TGG 0.540233 2_2:+56937720 Msa0259670:three_prime_UTR
GCTTTGGTTACATGCTTCCA+CGG 0.548483 2_2:-56936259 None:intergenic
TTATGATGATTGATGAGCAT+GGG 0.550009 2_2:-56937212 None:intergenic
TGTGCTGCATTTGCTCTCAA+AGG 0.550088 2_2:+56936462 Msa0259670:CDS
GTATATCTAATTCGTGCACA+TGG 0.551064 2_2:-56937370 None:intergenic
AAGGCTCGGAGACTCTGATT+GGG 0.552417 2_2:+56936122 Msa0259670:CDS
TTTATGATGATTGATGAGCA+TGG 0.552805 2_2:-56937213 None:intergenic
GGTTTAAGTCTGAGAGAAGC+GGG 0.553612 2_2:+56935730 Msa0259670:CDS
AATGTTCGTTGTCACGGCAG+TGG 0.554743 2_2:+56936578 Msa0259670:CDS
AAGTCTGAGAGAAGCGGGAA+TGG 0.555069 2_2:+56935735 Msa0259670:CDS
AGGGGACTCTTACTAGACTC+TGG 0.557413 2_2:-56935379 None:intergenic
CAGAGATGTGGCCGGTGGAG+AGG 0.557528 2_2:-56936183 None:intergenic
CTGGACCTAGAGTTCTTACT+GGG 0.557635 2_2:-56937013 None:intergenic
TGCTACCTCTACTGGTCTTG+CGG 0.560160 2_2:+56936147 Msa0259670:CDS
CAATACTTGGAGCTCTAGGT+GGG 0.562103 2_2:+56937084 Msa0259670:CDS
AAATTAGAGTACAGAAGCAC+GGG 0.563522 2_2:-56935586 None:intergenic
GTAACTAATTGTTGTTTACA+GGG 0.565538 2_2:-56935133 None:intergenic
GGACGGTGGGACAGTTCTGA+GGG 0.568449 2_2:-56935398 None:intergenic
AAGTTCAACTCTGCCAGTGT+TGG 0.569468 2_2:+56937710 Msa0259670:three_prime_UTR
GAACTAGCAGGAATTGCAGT+GGG 0.569469 2_2:+56936826 Msa0259670:CDS
CCAATACTTGGAGCTCTAGG+TGG 0.572700 2_2:+56937083 Msa0259670:CDS
ACCATATCCTCCGGAAGGAA+CGG 0.576185 2_2:-56936517 None:intergenic
AGCTAAGAGATAGACCCATA+TGG 0.576739 2_2:-56937060 None:intergenic
CCATCAACACCAGCAACACC+AGG 0.577155 2_2:+56935681 Msa0259670:CDS
TGGACGGTGGGACAGTTCTG+AGG 0.577190 2_2:-56935399 None:intergenic
CTGCATTGAAGCACTTCCCG+TGG 0.578067 2_2:+56936242 Msa0259670:CDS
CTTCACTCACTTAAAGAGCA+TGG 0.578332 2_2:-56935229 None:intergenic
TGGAAATTAGGAAAGAGGCT+TGG 0.583275 2_2:-56935359 None:intergenic
CATAACCGCAAGACCAGTAG+AGG 0.586587 2_2:-56936152 None:intergenic
CATTAATGAGCAGGTAACTG+TGG 0.587885 2_2:-56935841 None:intergenic
ATGATCATCATCCTCTCCAC+CGG 0.588983 2_2:+56936172 Msa0259670:CDS
CAAAAGAAATGGTGACTGCA+GGG 0.592708 2_2:-56936219 None:intergenic
AAAAGCTTGACCATATCCTC+CGG 0.599263 2_2:-56936526 None:intergenic
ACTTGTGCACCAATATACAT+AGG 0.600697 2_2:-56936426 None:intergenic
AATCAATGTCCCTATAAACT+CGG 0.603006 2_2:-56936053 None:intergenic
GAAATTAGAGTACAGAAGCA+CGG 0.609449 2_2:-56935587 None:intergenic
CTTTGGTTACATGCTTCCAC+GGG 0.610743 2_2:-56936258 None:intergenic
AGCACCAGAGATGTGGCCGG+TGG 0.615438 2_2:-56936188 None:intergenic
TGAGATGAGCACCAGAGATG+TGG 0.617163 2_2:-56936195 None:intergenic
CCGCCAACGAGTCCCAACAC+TGG 0.619707 2_2:-56937723 None:intergenic
TGCTGCATTTGCTCTCAAAG+GGG 0.620449 2_2:+56936464 Msa0259670:CDS
GGAGTCACCGTTCCTTCCGG+AGG 0.622617 2_2:+56936510 Msa0259670:CDS
ACTCACAGCTCTTGTGTCGG+TGG 0.623869 2_2:-56936601 None:intergenic
GGGTTTAAGTCTGAGAGAAG+CGG 0.625558 2_2:+56935729 Msa0259670:CDS
ATGTAGGTAGGAGAACTAGC+AGG 0.625936 2_2:+56936814 Msa0259670:intron
ATTAATGAGCAGGTAACTGT+GGG 0.626119 2_2:-56935840 None:intergenic
TAGCAGCATGTGGAAGTGGA+CGG 0.626553 2_2:-56935415 None:intergenic
AGAACTAGCAGGAATTGCAG+TGG 0.628860 2_2:+56936825 Msa0259670:CDS
CATGCTCTTTAAGTGAGTGA+AGG 0.634040 2_2:+56935230 Msa0259670:five_prime_UTR
GTTGAACTTACGTCTTTGTG+GGG 0.634464 2_2:-56937696 None:intergenic
AGCATGTGGAAGTGGACGGT+GGG 0.635263 2_2:-56935411 None:intergenic
GGCTTGGTTCACACTACACT+AGG 0.639934 2_2:-56935343 None:intergenic
GCAATCTGCTATGATCCTTG+AGG 0.641389 2_2:+56937746 Msa0259670:three_prime_UTR
TTGATCATCTTCTAAAGCTG+GGG 0.642052 2_2:-56937178 None:intergenic
GAACCAAAAGACACAAGGCT+CGG 0.650309 2_2:+56936108 Msa0259670:CDS
GCTTGACCATATCCTCCGGA+AGG 0.651188 2_2:-56936522 None:intergenic
TATGATGATTGATGAGCATG+GGG 0.652693 2_2:-56937211 None:intergenic
CAGCATGTGGAAGTGGACGG+TGG 0.655771 2_2:-56935412 None:intergenic
AATACTTGGAGCTCTAGGTG+GGG 0.656773 2_2:+56937085 Msa0259670:CDS
TGCTCAATATACTAATAGCA+GGG 0.657754 2_2:+56936860 Msa0259670:CDS
GAACACAAAAGGAATGACCA+AGG 0.662028 2_2:+56935286 Msa0259670:five_prime_UTR
AATACTCACAGCTCTTGTGT+CGG 0.671145 2_2:-56936604 None:intergenic
ATGAGCACCAGAGATGTGGC+CGG 0.675762 2_2:-56936191 None:intergenic
ATTGTGAACCAAAAGACACA+AGG 0.703696 2_2:+56936103 Msa0259670:CDS
GACGGTGGGACAGTTCTGAG+GGG 0.726625 2_2:-56935397 None:intergenic
ATGATGATTGATGAGCATGG+GGG 0.825649 2_2:-56937210 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATCATGATTAATTAAT+TGG + chr2_2:56935206-56935225 Msa0259670:five_prime_UTR 10.0%
!! TAATTTCAACAACTTTAATT+AGG - chr2_2:56936311-56936330 None:intergenic 15.0%
!! AAAGTATAAATTATAATTGC+AGG + chr2_2:56936401-56936420 Msa0259670:intron 15.0%
!!! CATATGTTCAAATTTTAATT+TGG - chr2_2:56936729-56936748 None:intergenic 15.0%
!!! ATATATATATAAGTTTTGTG+TGG + chr2_2:56937321-56937340 Msa0259670:three_prime_UTR 15.0%
!! ATTAGAGATAATTAATTACT+TGG - chr2_2:56937556-56937575 None:intergenic 15.0%
!! TTAATTATCTCTAATTACAT+AGG + chr2_2:56937561-56937580 Msa0259670:three_prime_UTR 15.0%
!! AATTTACATTAGACAAGAAA+AGG - chr2_2:56935617-56935636 None:intergenic 20.0%
!! AATGGAACATATTAATAATG+AGG + chr2_2:56935653-56935672 Msa0259670:exon 20.0%
!! ACAACTTTAATTAGGAAATT+AGG - chr2_2:56936303-56936322 None:intergenic 20.0%
!! TATTAGCTAAAAGTTACTAT+AGG - chr2_2:56936634-56936653 None:intergenic 20.0%
!! TTAAAATTTGAACATATGCT+TGG + chr2_2:56936731-56936750 Msa0259670:intron 20.0%
!! ATAACCATAAACATCAATTA+GGG - chr2_2:56936796-56936815 None:intergenic 20.0%
!!! AATATTTTATCTGTTGCTAA+TGG - chr2_2:56937593-56937612 None:intergenic 20.0%
!! ATTAGCAACAGATAAAATAT+TGG + chr2_2:56937592-56937611 Msa0259670:three_prime_UTR 20.0%
!! TTTCAAAGTAAAGTTATTGA+TGG + chr2_2:56937819-56937838 Msa0259670:three_prime_UTR 20.0%
!! TTCAAAGTAAAGTTATTGAT+GGG + chr2_2:56937820-56937839 Msa0259670:three_prime_UTR 20.0%
!!! CATTTTTCATTAGCTTTTTT+CGG + chr2_2:56937950-56937969 Msa0259670:three_prime_UTR 20.0%
!!! GTAACTAATTGTTGTTTACA+GGG - chr2_2:56935136-56935155 None:intergenic 25.0%
!!! AGTAACTAATTGTTGTTTAC+AGG - chr2_2:56935137-56935156 None:intergenic 25.0%
! GAAACTGATATATGAGAAAA+AGG - chr2_2:56935441-56935460 None:intergenic 25.0%
!! TTATTGTTGCTATTGTATGT+CGG - chr2_2:56935559-56935578 None:intergenic 25.0%
!!! ATATATACCTTTTTTGCAAG+AGG - chr2_2:56935885-56935904 None:intergenic 25.0%
! ATTTATGTGTGTCTAATCTT+TGG + chr2_2:56935943-56935962 Msa0259670:intron 25.0%
! AGTTTGAAAAGAAAAAGAGA+AGG + chr2_2:56936672-56936691 Msa0259670:intron 25.0%
! CATAACCATAAACATCAATT+AGG - chr2_2:56936797-56936816 None:intergenic 25.0%
! ATTGATGTTTATGGTTATGT+AGG + chr2_2:56936798-56936817 Msa0259670:intron 25.0%
! TATATATGTTCATGACAAGT+GGG - chr2_2:56936948-56936967 None:intergenic 25.0%
! ATATATATGTTCATGACAAG+TGG - chr2_2:56936949-56936968 None:intergenic 25.0%
! TGTCATGAACATATATATGA+TGG + chr2_2:56936951-56936970 Msa0259670:intron 25.0%
! AAATAACTACAAAGCCATAT+GGG + chr2_2:56937046-56937065 Msa0259670:CDS 25.0%
! TCATCATAAAATGACACTAA+AGG + chr2_2:56937226-56937245 Msa0259670:three_prime_UTR 25.0%
! AAAAACAAGCCAAAATCATT+TGG + chr2_2:56937347-56937366 Msa0259670:three_prime_UTR 25.0%
!! TCTTTTACTTTAACCATGTA+AGG - chr2_2:56937518-56937537 None:intergenic 25.0%
! TACATGGTTAAAGTAAAAGA+TGG + chr2_2:56937518-56937537 Msa0259670:three_prime_UTR 25.0%
TTATCCTAATTCTTGAGCAA+TGG + chr2_2:56935087-56935106 Msa0259670:five_prime_UTR 30.0%
AATACACTCTCATGATACTT+AGG + chr2_2:56935177-56935196 Msa0259670:five_prime_UTR 30.0%
CTACAAACTTGCAGAAATTA+AGG + chr2_2:56935262-56935281 Msa0259670:five_prime_UTR 30.0%
GAAATTAAGGAGAACACAAA+AGG + chr2_2:56935275-56935294 Msa0259670:five_prime_UTR 30.0%
TTTCCATTCATCAATTTCCT+TGG - chr2_2:56935306-56935325 None:intergenic 30.0%
CACTAGGCATCAATTATATA+CGG - chr2_2:56935330-56935349 None:intergenic 30.0%
AATGAAACGAAGCAAATTAG+TGG - chr2_2:56935466-56935485 None:intergenic 30.0%
! TATTGTTGCTATTGTATGTC+GGG - chr2_2:56935558-56935577 None:intergenic 30.0%
! TTTACCATTGACTGTAGAAA+TGG + chr2_2:56935635-56935654 Msa0259670:exon 30.0%
GCATTTACAATTCTTGAGTA+GGG - chr2_2:56935781-56935800 None:intergenic 30.0%
AGCATTTACAATTCTTGAGT+AGG - chr2_2:56935782-56935801 None:intergenic 30.0%
CTCAAGAATTGTAAATGCTT+CGG + chr2_2:56935783-56935802 Msa0259670:CDS 30.0%
TACTTAGCACTTCAATGATA+TGG - chr2_2:56935989-56936008 None:intergenic 30.0%
AATCAATGTCCCTATAAACT+CGG - chr2_2:56936056-56936075 None:intergenic 30.0%
TAATTAGGAAATTAGGCTGA+AGG - chr2_2:56936296-56936315 None:intergenic 30.0%
TAATCTAATGTTCGTTGTCA+CGG + chr2_2:56936572-56936591 Msa0259670:CDS 30.0%
ATGTTTATGGTTATGTAGGT+AGG + chr2_2:56936802-56936821 Msa0259670:intron 30.0%
ATGCTCAATATACTAATAGC+AGG + chr2_2:56936859-56936878 Msa0259670:CDS 30.0%
TGCTCAATATACTAATAGCA+GGG + chr2_2:56936860-56936879 Msa0259670:CDS 30.0%
ATATATGTTCATGACAAGTG+GGG - chr2_2:56936947-56936966 None:intergenic 30.0%
CAAATAACTACAAAGCCATA+TGG + chr2_2:56937045-56937064 Msa0259670:CDS 30.0%
TTTGCATTATCATCTTCTTC+GGG - chr2_2:56937137-56937156 None:intergenic 30.0%
CTTTGCATTATCATCTTCTT+CGG - chr2_2:56937138-56937157 None:intergenic 30.0%
TTATGATGATTGATGAGCAT+GGG - chr2_2:56937215-56937234 None:intergenic 30.0%
TTTATGATGATTGATGAGCA+TGG - chr2_2:56937216-56937235 None:intergenic 30.0%
! CTATTTTCTTTCTCCTTACA+TGG + chr2_2:56937502-56937521 Msa0259670:three_prime_UTR 30.0%
ATAAGTTATAACACCCTGTT+TGG + chr2_2:56937902-56937921 Msa0259670:three_prime_UTR 30.0%
TAAGTTATAACACCCTGTTT+GGG + chr2_2:56937903-56937922 Msa0259670:three_prime_UTR 30.0%
AGCTAATGAAAAATGTCACA+AGG - chr2_2:56937945-56937964 None:intergenic 30.0%
TGACCAAGGAAATTGATGAA+TGG + chr2_2:56935300-56935319 Msa0259670:five_prime_UTR 35.0%
! CTTACTAGACTCTGGAAATT+AGG - chr2_2:56935374-56935393 None:intergenic 35.0%
TAGCATGACTAGTATCACTA+TGG - chr2_2:56935490-56935509 None:intergenic 35.0%
! AAATTAGAGTACAGAAGCAC+GGG - chr2_2:56935589-56935608 None:intergenic 35.0%
GAAATTAGAGTACAGAAGCA+CGG - chr2_2:56935590-56935609 None:intergenic 35.0%
TGTTCCATTTCTACAGTCAA+TGG - chr2_2:56935642-56935661 None:intergenic 35.0%
CATCTTCTAAGTTCCATTCT+TGG - chr2_2:56935812-56935831 None:intergenic 35.0%
CAAGAATGGAACTTAGAAGA+TGG + chr2_2:56935810-56935829 Msa0259670:CDS 35.0%
ATTAATGAGCAGGTAACTGT+GGG - chr2_2:56935843-56935862 None:intergenic 35.0%
! ATCTGTAACACAGGTAGTTT+TGG - chr2_2:56936028-56936047 None:intergenic 35.0%
AAACTACCTGTGTTACAGAT+AGG + chr2_2:56936028-56936047 Msa0259670:intron 35.0%
!! ACATTGATTCTGATGTTTGC+TGG + chr2_2:56936067-56936086 Msa0259670:CDS 35.0%
!! CATTGATTCTGATGTTTGCT+GGG + chr2_2:56936068-56936087 Msa0259670:CDS 35.0%
ATTGTGAACCAAAAGACACA+AGG + chr2_2:56936103-56936122 Msa0259670:CDS 35.0%
CTTCAATGCAGCAAAAGAAA+TGG - chr2_2:56936233-56936252 None:intergenic 35.0%
GAAGGATTAAGGAAAAGCTT+TGG - chr2_2:56936278-56936297 None:intergenic 35.0%
GAAATTAGGCTGAAGGATTA+AGG - chr2_2:56936289-56936308 None:intergenic 35.0%
ACTTGTGCACCAATATACAT+AGG - chr2_2:56936429-56936448 None:intergenic 35.0%
! GTATATTGGTGCACAAGTTT+TGG + chr2_2:56936431-56936450 Msa0259670:CDS 35.0%
! GTTTTTCACCCTTTTATGAG+TGG + chr2_2:56936486-56936505 Msa0259670:CDS 35.0%
GATGATATTATCCCCTATGT+CGG - chr2_2:56936707-56936726 None:intergenic 35.0%
GAGTCCCTAATTGATGTTTA+TGG + chr2_2:56936789-56936808 Msa0259670:intron 35.0%
TAGTTATTTGCAGCTATAGC+TGG - chr2_2:56937035-56937054 None:intergenic 35.0%
TATCTCTTAGCTCCAATACT+TGG + chr2_2:56937071-56937090 Msa0259670:CDS 35.0%
!! TTGATCATCTTCTAAAGCTG+GGG - chr2_2:56937181-56937200 None:intergenic 35.0%
!! GTTGATCATCTTCTAAAGCT+GGG - chr2_2:56937182-56937201 None:intergenic 35.0%
!! AGTTGATCATCTTCTAAAGC+TGG - chr2_2:56937183-56937202 None:intergenic 35.0%
TATGATGATTGATGAGCATG+GGG - chr2_2:56937214-56937233 None:intergenic 35.0%
GTATATCTAATTCGTGCACA+TGG - chr2_2:56937373-56937392 None:intergenic 35.0%
AATTGAGTGCTCTTTCAGTT+TGG + chr2_2:56937455-56937474 Msa0259670:three_prime_UTR 35.0%
AACTCTTGATTAGAAAGAGC+TGG + chr2_2:56937639-56937658 Msa0259670:three_prime_UTR 35.0%
AGTTGAACTTACGTCTTTGT+GGG - chr2_2:56937700-56937719 None:intergenic 35.0%
! TTTTCCTTCAATAAGCCTCA+AGG - chr2_2:56937764-56937783 None:intergenic 35.0%
! TTCACAAGTTTTCCCAAACA+GGG - chr2_2:56937918-56937937 None:intergenic 35.0%
! TTTCACAAGTTTTCCCAAAC+AGG - chr2_2:56937919-56937938 None:intergenic 35.0%
TTCATAAGCTATCTTGGAGA+AGG - chr2_2:56937988-56938007 None:intergenic 35.0%
! TGTGCCATTGCTCAAGAATT+AGG - chr2_2:56935094-56935113 None:intergenic 40.0%
CTTCACTCACTTAAAGAGCA+TGG - chr2_2:56935232-56935251 None:intergenic 40.0%
CATGCTCTTTAAGTGAGTGA+AGG + chr2_2:56935230-56935249 Msa0259670:five_prime_UTR 40.0%
GAACACAAAAGGAATGACCA+AGG + chr2_2:56935286-56935305 Msa0259670:five_prime_UTR 40.0%
TGGAAATTAGGAAAGAGGCT+TGG - chr2_2:56935362-56935381 None:intergenic 40.0%
! GACTCTGGAAATTAGGAAAG+AGG - chr2_2:56935367-56935386 None:intergenic 40.0%
GACTTAAACCCACCAAAAAG+AGG - chr2_2:56935720-56935739 None:intergenic 40.0%
CATTAATGAGCAGGTAACTG+TGG - chr2_2:56935844-56935863 None:intergenic 40.0%
AGATAGGAGCCGAGTTTATA+GGG + chr2_2:56936044-56936063 Msa0259670:intron 40.0%
!!! CTTTTGGTTCACAATAGCAG+TGG - chr2_2:56936098-56936117 None:intergenic 40.0%
CAAAAGAAATGGTGACTGCA+GGG - chr2_2:56936222-56936241 None:intergenic 40.0%
TTGCAGGTGCCTATGTATAT+TGG + chr2_2:56936417-56936436 Msa0259670:intron 40.0%
! TTTCACCCTTTTATGAGTGG+TGG + chr2_2:56936489-56936508 Msa0259670:CDS 40.0%
TGACTCCACCACTCATAAAA+GGG - chr2_2:56936497-56936516 None:intergenic 40.0%
AAAAGCTTGACCATATCCTC+CGG - chr2_2:56936529-56936548 None:intergenic 40.0%
AATACTCACAGCTCTTGTGT+CGG - chr2_2:56936607-56936626 None:intergenic 40.0%
TTAATTGCTCTTCACGCGAA+GGG - chr2_2:56936767-56936786 None:intergenic 40.0%
GAGAGTGATAAGTGAGAGTT+AGG - chr2_2:56936897-56936916 None:intergenic 40.0%
TTCATTGACGCTCCTGTTAT+TGG - chr2_2:56936993-56937012 None:intergenic 40.0%
ATGAACCCAGTAAGAACTCT+AGG + chr2_2:56937008-56937027 Msa0259670:CDS 40.0%
AGCTAAGAGATAGACCCATA+TGG - chr2_2:56937063-56937082 None:intergenic 40.0%
ATGATGATTGATGAGCATGG+GGG - chr2_2:56937213-56937232 None:intergenic 40.0%
! TGCACATGGCCAAATGATTT+TGG - chr2_2:56937359-56937378 None:intergenic 40.0%
! AGCACTCAATTTTGCTTGCT+TGG - chr2_2:56937446-56937465 None:intergenic 40.0%
GTTGAACTTACGTCTTTGTG+GGG - chr2_2:56937699-56937718 None:intergenic 40.0%
GAGTTGAACTTACGTCTTTG+TGG - chr2_2:56937701-56937720 None:intergenic 40.0%
TGATCCTTGAGGCTTATTGA+AGG + chr2_2:56937757-56937776 Msa0259670:three_prime_UTR 40.0%
GAGAAAAAGGTAGCAGCATG+TGG - chr2_2:56935428-56935447 None:intergenic 45.0%
! ACTCCTGGTGTACCTCTTTT+TGG + chr2_2:56935705-56935724 Msa0259670:CDS 45.0%
!! CTGGTGTACCTCTTTTTGGT+GGG + chr2_2:56935709-56935728 Msa0259670:CDS 45.0%
GGGTTTAAGTCTGAGAGAAG+CGG + chr2_2:56935729-56935748 Msa0259670:CDS 45.0%
GGTTTAAGTCTGAGAGAAGC+GGG + chr2_2:56935730-56935749 Msa0259670:CDS 45.0%
!! AATGCTTCGGTGTCCAAGAA+TGG + chr2_2:56935796-56935815 Msa0259670:CDS 45.0%
!!! TTTTTTGCAAGAGGCACAGG+AGG - chr2_2:56935876-56935895 None:intergenic 45.0%
TCCTGTGCCTCTTGCAAAAA+AGG + chr2_2:56935875-56935894 Msa0259670:CDS 45.0%
!!! ACCTTTTTTGCAAGAGGCAC+AGG - chr2_2:56935879-56935898 None:intergenic 45.0%
CAGATAGGAGCCGAGTTTAT+AGG + chr2_2:56936043-56936062 Msa0259670:intron 45.0%
GAACCAAAAGACACAAGGCT+CGG + chr2_2:56936108-56936127 Msa0259670:CDS 45.0%
ATGATCATCATCCTCTCCAC+CGG + chr2_2:56936172-56936191 Msa0259670:CDS 45.0%
GCAAAAGAAATGGTGACTGC+AGG - chr2_2:56936223-56936242 None:intergenic 45.0%
CTTTGGTTACATGCTTCCAC+GGG - chr2_2:56936261-56936280 None:intergenic 45.0%
GCTTTGGTTACATGCTTCCA+CGG - chr2_2:56936262-56936281 None:intergenic 45.0%
TGTGCTGCATTTGCTCTCAA+AGG + chr2_2:56936462-56936481 Msa0259670:CDS 45.0%
GTGCTGCATTTGCTCTCAAA+GGG + chr2_2:56936463-56936482 Msa0259670:CDS 45.0%
TGCTGCATTTGCTCTCAAAG+GGG + chr2_2:56936464-56936483 Msa0259670:CDS 45.0%
GTGACTCCACCACTCATAAA+AGG - chr2_2:56936498-56936517 None:intergenic 45.0%
AAGGAAGATGAAGCCGACAT+AGG + chr2_2:56936691-56936710 Msa0259670:intron 45.0%
AGGAAGATGAAGCCGACATA+GGG + chr2_2:56936692-56936711 Msa0259670:intron 45.0%
TAATTGCTCTTCACGCGAAG+GGG - chr2_2:56936766-56936785 None:intergenic 45.0%
GTTAATTGCTCTTCACGCGA+AGG - chr2_2:56936768-56936787 None:intergenic 45.0%
ATGTAGGTAGGAGAACTAGC+AGG + chr2_2:56936814-56936833 Msa0259670:intron 45.0%
AGAACTAGCAGGAATTGCAG+TGG + chr2_2:56936825-56936844 Msa0259670:CDS 45.0%
GAACTAGCAGGAATTGCAGT+GGG + chr2_2:56936826-56936845 Msa0259670:CDS 45.0%
TTCATGACAAGTGGGGATAG+GGG - chr2_2:56936940-56936959 None:intergenic 45.0%
GTTCATGACAAGTGGGGATA+GGG - chr2_2:56936941-56936960 None:intergenic 45.0%
TGTTCATGACAAGTGGGGAT+AGG - chr2_2:56936942-56936961 None:intergenic 45.0%
CTGGACCTAGAGTTCTTACT+GGG - chr2_2:56937016-56937035 None:intergenic 45.0%
!! CAATACTTGGAGCTCTAGGT+GGG + chr2_2:56937084-56937103 Msa0259670:CDS 45.0%
!! AATACTTGGAGCTCTAGGTG+GGG + chr2_2:56937085-56937104 Msa0259670:CDS 45.0%
AAGTTCAACTCTGCCAGTGT+TGG + chr2_2:56937710-56937729 Msa0259670:three_prime_UTR 45.0%
AGTTCAACTCTGCCAGTGTT+GGG + chr2_2:56937711-56937730 Msa0259670:three_prime_UTR 45.0%
GCAATCTGCTATGATCCTTG+AGG + chr2_2:56937746-56937765 Msa0259670:three_prime_UTR 45.0%
! TTGATGGGCAATCTGCTTTG+AGG + chr2_2:56937835-56937854 Msa0259670:three_prime_UTR 45.0%
! TTTGAGGCATGATGCCTGTT+TGG + chr2_2:56937851-56937870 Msa0259670:three_prime_UTR 45.0%
TCAAATCAGCCCATCCAAAC+AGG - chr2_2:56937868-56937887 None:intergenic 45.0%
! GGCTTGGTTCACACTACACT+AGG - chr2_2:56935346-56935365 None:intergenic 50.0%
AGGGGACTCTTACTAGACTC+TGG - chr2_2:56935382-56935401 None:intergenic 50.0%
TAGCAGCATGTGGAAGTGGA+CGG - chr2_2:56935418-56935437 None:intergenic 50.0%
AAGGTAGCAGCATGTGGAAG+TGG - chr2_2:56935422-56935441 None:intergenic 50.0%
! CCACCAAAAAGAGGTACACC+AGG - chr2_2:56935711-56935730 None:intergenic 50.0%
! CCTGGTGTACCTCTTTTTGG+TGG + chr2_2:56935708-56935727 Msa0259670:CDS 50.0%
AAGTCTGAGAGAAGCGGGAA+TGG + chr2_2:56935735-56935754 Msa0259670:CDS 50.0%
TCGGCTCCTATCTGTAACAC+AGG - chr2_2:56936037-56936056 None:intergenic 50.0%
!! TCTCCGAGCCTTGTGTCTTT+TGG - chr2_2:56936114-56936133 None:intergenic 50.0%
AAGGCTCGGAGACTCTGATT+GGG + chr2_2:56936122-56936141 Msa0259670:CDS 50.0%
ATTGGGTGTGCTACCTCTAC+TGG + chr2_2:56936139-56936158 Msa0259670:CDS 50.0%
!! TGCTACCTCTACTGGTCTTG+CGG + chr2_2:56936147-56936166 Msa0259670:CDS 50.0%
CATAACCGCAAGACCAGTAG+AGG - chr2_2:56936155-56936174 None:intergenic 50.0%
TGAGATGAGCACCAGAGATG+TGG - chr2_2:56936198-56936217 None:intergenic 50.0%
ACCATATCCTCCGGAAGGAA+CGG - chr2_2:56936520-56936539 None:intergenic 50.0%
AATGTTCGTTGTCACGGCAG+TGG + chr2_2:56936578-56936597 Msa0259670:CDS 50.0%
GGAAGATGAAGCCGACATAG+GGG + chr2_2:56936693-56936712 Msa0259670:intron 50.0%
TCATGACAAGTGGGGATAGG+GGG - chr2_2:56936939-56936958 None:intergenic 50.0%
GCTGGACCTAGAGTTCTTAC+TGG - chr2_2:56937017-56937036 None:intergenic 50.0%
GCTCCAATACTTGGAGCTCT+AGG + chr2_2:56937080-56937099 Msa0259670:CDS 50.0%
CCACCTAGAGCTCCAAGTAT+TGG - chr2_2:56937086-56937105 None:intergenic 50.0%
!! CCAATACTTGGAGCTCTAGG+TGG + chr2_2:56937083-56937102 Msa0259670:CDS 50.0%
AGGCATGATGCCTGTTTGGA+TGG + chr2_2:56937855-56937874 Msa0259670:three_prime_UTR 50.0%
GGCATGATGCCTGTTTGGAT+GGG + chr2_2:56937856-56937875 Msa0259670:three_prime_UTR 50.0%
AGCATGTGGAAGTGGACGGT+GGG - chr2_2:56935414-56935433 None:intergenic 55.0%
!! CCTGGTGTTGCTGGTGTTGA+TGG - chr2_2:56935684-56935703 None:intergenic 55.0%
CCATCAACACCAGCAACACC+AGG + chr2_2:56935681-56935700 Msa0259670:CDS 55.0%
AGGGGGTGGCATTAATGAGC+AGG - chr2_2:56935853-56935872 None:intergenic 55.0%
! TTGCAAGAGGCACAGGAGGA+GGG - chr2_2:56935872-56935891 None:intergenic 55.0%
! TTTGCAAGAGGCACAGGAGG+AGG - chr2_2:56935873-56935892 None:intergenic 55.0%
CAAGGCTCGGAGACTCTGAT+TGG + chr2_2:56936121-56936140 Msa0259670:CDS 55.0%
ATGAGCACCAGAGATGTGGC+CGG - chr2_2:56936194-56936213 None:intergenic 55.0%
! CTGCATTGAAGCACTTCCCG+TGG + chr2_2:56936242-56936261 Msa0259670:CDS 55.0%
ACCGTTCCTTCCGGAGGATA+TGG + chr2_2:56936516-56936535 Msa0259670:CDS 55.0%
GCTTGACCATATCCTCCGGA+AGG - chr2_2:56936525-56936544 None:intergenic 55.0%
ACTCACAGCTCTTGTGTCGG+TGG - chr2_2:56936604-56936623 None:intergenic 55.0%
AAGTGGGGATAGGGGGAAGA+AGG - chr2_2:56936932-56936951 None:intergenic 55.0%
! ATGATGGTGCACTGCGTTGC+AGG + chr2_2:56936967-56936986 Msa0259670:intron 55.0%
CTGCGTTGCAGGCCAATAAC+AGG + chr2_2:56936978-56936997 Msa0259670:intron 55.0%
!! GACGGTGGGACAGTTCTGAG+GGG - chr2_2:56935400-56935419 None:intergenic 60.0%
! GGACGGTGGGACAGTTCTGA+GGG - chr2_2:56935401-56935420 None:intergenic 60.0%
TGGACGGTGGGACAGTTCTG+AGG - chr2_2:56935402-56935421 None:intergenic 60.0%
CAGCATGTGGAAGTGGACGG+TGG - chr2_2:56935415-56935434 None:intergenic 60.0%
!! AGAGGTACACCAGGAGTGCC+TGG - chr2_2:56935702-56935721 None:intergenic 60.0%
! TGCAAGAGGCACAGGAGGAG+GGG - chr2_2:56935871-56935890 None:intergenic 60.0%
GGTGGAGTCACCGTTCCTTC+CGG + chr2_2:56936507-56936526 Msa0259670:CDS 60.0%
! CTGCCAGTGTTGGGACTCGT+TGG + chr2_2:56937720-56937739 Msa0259670:three_prime_UTR 60.0%
! CCAGTGTTGGGACTCGTTGG+CGG + chr2_2:56937723-56937742 Msa0259670:three_prime_UTR 60.0%
! CAGTGTTGGGACTCGTTGGC+GGG + chr2_2:56937724-56937743 Msa0259670:three_prime_UTR 60.0%
!! CCAGGAGTGCCTGGTGTTGC+TGG - chr2_2:56935693-56935712 None:intergenic 65.0%
CCAGCAACACCAGGCACTCC+TGG + chr2_2:56935690-56935709 Msa0259670:CDS 65.0%
! GCAAGAGGCACAGGAGGAGG+GGG - chr2_2:56935870-56935889 None:intergenic 65.0%
CAGAGATGTGGCCGGTGGAG+AGG - chr2_2:56936186-56936205 None:intergenic 65.0%
CTCTCCACCGGCCACATCTC+TGG + chr2_2:56936184-56936203 Msa0259670:CDS 65.0%
AGCACCAGAGATGTGGCCGG+TGG - chr2_2:56936191-56936210 None:intergenic 65.0%
GGAGTCACCGTTCCTTCCGG+AGG + chr2_2:56936510-56936529 Msa0259670:CDS 65.0%
!! CTTGGAGCTCTAGGTGGGGC+AGG + chr2_2:56937089-56937108 Msa0259670:CDS 65.0%
CCGCCAACGAGTCCCAACAC+TGG - chr2_2:56937726-56937745 None:intergenic 65.0%
! AGAGGCACAGGAGGAGGGGG+TGG - chr2_2:56935867-56935886 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 56935058 56938010 56935058 ID=Msa0259670;Name=Msa0259670
chr2_2 mRNA 56935058 56938010 56935058 ID=Msa0259670-mRNA-1;Parent=Msa0259670;Name=Msa0259670-mRNA-1;_AED=0.05;_eAED=0.05;_QI=596|1|1|1|0.8|0.66|6|768|310
chr2_2 exon 56935058 56935896 56935058 ID=Msa0259670-mRNA-1:exon:17225;Parent=Msa0259670-mRNA-1
chr2_2 exon 56936046 56936270 56936046 ID=Msa0259670-mRNA-1:exon:17226;Parent=Msa0259670-mRNA-1
chr2_2 exon 56936423 56936617 56936423 ID=Msa0259670-mRNA-1:exon:17227;Parent=Msa0259670-mRNA-1
chr2_2 exon 56936820 56936881 56936820 ID=Msa0259670-mRNA-1:exon:17228;Parent=Msa0259670-mRNA-1
chr2_2 exon 56936989 56937262 56936989 ID=Msa0259670-mRNA-1:exon:17229;Parent=Msa0259670-mRNA-1
chr2_2 exon 56937309 56938010 56937309 ID=Msa0259670-mRNA-1:exon:17230;Parent=Msa0259670-mRNA-1
chr2_2 five_prime_UTR 56935058 56935653 56935058 ID=Msa0259670-mRNA-1:five_prime_utr;Parent=Msa0259670-mRNA-1
chr2_2 CDS 56935654 56935896 56935654 ID=Msa0259670-mRNA-1:cds;Parent=Msa0259670-mRNA-1
chr2_2 CDS 56936046 56936270 56936046 ID=Msa0259670-mRNA-1:cds;Parent=Msa0259670-mRNA-1
chr2_2 CDS 56936423 56936617 56936423 ID=Msa0259670-mRNA-1:cds;Parent=Msa0259670-mRNA-1
chr2_2 CDS 56936820 56936881 56936820 ID=Msa0259670-mRNA-1:cds;Parent=Msa0259670-mRNA-1
chr2_2 CDS 56936989 56937196 56936989 ID=Msa0259670-mRNA-1:cds;Parent=Msa0259670-mRNA-1
chr2_2 three_prime_UTR 56937197 56937262 56937197 ID=Msa0259670-mRNA-1:three_prime_utr;Parent=Msa0259670-mRNA-1
chr2_2 three_prime_UTR 56937309 56938010 56937309 ID=Msa0259670-mRNA-1:three_prime_utr;Parent=Msa0259670-mRNA-1
Gene Sequence

>Msa0259670

ATGGAACATATTAATAATGAGGAAATTCCATCAACACCAGCAACACCAGGCACTCCTGGTGTACCTCTTTTTGGTGGGTTTAAGTCTGAGAGAAGCGGGAATGGTAATAATAAATATAAGAAGTCCCTACTCAAGAATTGTAAATGCTTCGGTGTCCAAGAATGGAACTTAGAAGATGGAGCTCTACCCACAGTTACCTGCTCATTAATGCCACCCCCTCCTCCTGTGCCTCTTGCAAAAAAGATAGGAGCCGAGTTTATAGGGACATTGATTCTGATGTTTGCTGGGACAGCCACTGCTATTGTGAACCAAAAGACACAAGGCTCGGAGACTCTGATTGGGTGTGCTACCTCTACTGGTCTTGCGGTTATGATCATCATCCTCTCCACCGGCCACATCTCTGGTGCTCATCTCAACCCTGCAGTCACCATTTCTTTTGCTGCATTGAAGCACTTCCCGTGGAAGCATGTGCCTATGTATATTGGTGCACAAGTTTTGGCATCAATATGTGCTGCATTTGCTCTCAAAGGGGTTTTTCACCCTTTTATGAGTGGTGGAGTCACCGTTCCTTCCGGAGGATATGGTCAAGCTTTTGCTTTAGAATTCATTATCAGCTTTAATCTAATGTTCGTTGTCACGGCAGTGGCCACCGACACAAGAGCTGTAGGAGAACTAGCAGGAATTGCAGTGGGAGCAACTGTGATGCTCAATATACTAATAGCAGGGCCAATAACAGGAGCGTCAATGAACCCAGTAAGAACTCTAGGTCCAGCTATAGCTGCAAATAACTACAAAGCCATATGGGTCTATCTCTTAGCTCCAATACTTGGAGCTCTAGGTGGGGCAGGAACTTATACTGCAGTCAAGCTTCCCGAAGAAGATGATAATGCAAAGACTAATGCTTCTTCAAATCACCCCAGCTTTAGAAGATGA
Protein sequence

>Msa0259670

MEHINNEEIPSTPATPGTPGVPLFGGFKSERSGNGNNKYKKSLLKNCKCFGVQEWNLEDGALPTVTCSLMPPPPPVPLAKKIGAEFIGTLILMFAGTATAIVNQKTQGSETLIGCATSTGLAVMIIILSTGHISGAHLNPAVTISFAALKHFPWKHVPMYIGAQVLASICAAFALKGVFHPFMSGGVTVPSGGYGQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAGPITGASMNPVRTLGPAIAANNYKAIWVYLLAPILGALGGAGTYTAVKLPEEDDNAKTNASSNHPSFRR*