AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0341490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0341490 MtrunA17_Chr2g0333771 98.387 310 5 0 1 310 1 310 0.0 619
Msa0341490 MtrunA17_Chr4g0001221 83.548 310 43 3 4 310 1 305 0.0 506
Msa0341490 MtrunA17_Chr4g0001111 83.784 222 28 3 4 222 1 217 1.40e-128 365
Msa0341490 MtrunA17_Chr1g0201331 58.224 304 107 4 11 310 13 300 3.45e-110 322
Msa0341490 MtrunA17_Chr4g0001161 83.571 140 23 0 93 232 1 140 1.29e-77 233
Msa0341490 MtrunA17_Chr8g0379901 48.473 262 130 2 45 303 4 263 1.18e-76 235
Msa0341490 MtrunA17_Chr8g0379911 51.542 227 105 2 80 303 38 262 1.11e-74 230
Msa0341490 MtrunA17_Chr2g0284631 47.710 262 133 2 53 310 64 325 4.71e-74 230
Msa0341490 MtrunA17_Chr2g0284591 49.206 252 119 3 56 303 14 260 6.24e-73 226
Msa0341490 MtrunA17_Chr2g0284611 49.206 252 119 3 56 303 16 262 2.48e-72 224
Msa0341490 MtrunA17_Chr7g0217331 46.121 232 122 1 75 306 32 260 1.50e-71 222
Msa0341490 MtrunA17_Chr2g0284581 51.584 221 102 2 76 293 34 252 1.70e-69 217
Msa0341490 MtrunA17_Chr2g0284601 50.485 206 97 2 91 293 1 204 1.31e-61 195
Msa0341490 MtrunA17_Chr4g0001151 77.863 131 14 1 93 223 1 116 2.47e-60 195
Msa0341490 MtrunA17_Chr4g0044201 42.045 264 128 5 56 310 8 255 4.12e-60 192
Msa0341490 MtrunA17_Chr5g0435051 45.495 222 116 3 76 293 36 256 1.99e-57 186
Msa0341490 MtrunA17_Chr3g0079421 47.685 216 109 3 76 289 22 235 2.37e-55 180
Msa0341490 MtrunA17_Chr5g0426131 43.779 217 116 2 80 292 37 251 4.14e-55 180
Msa0341490 MtrunA17_Chr1g0184541 40.175 229 137 0 78 306 60 288 4.95e-51 170
Msa0341490 MtrunA17_Chr4g0001121 82.022 89 16 0 222 310 10 98 2.14e-43 144
Msa0341490 MtrunA17_Chr2g0331261 39.286 224 116 8 84 293 24 241 2.47e-32 120
Msa0341490 MtrunA17_Chr1g0146161 37.073 205 109 7 85 277 24 220 4.79e-29 111
Msa0341490 MtrunA17_Chr8g0340161 35.749 207 115 4 84 277 23 224 3.10e-27 106
Msa0341490 MtrunA17_Chr1g0188771 34.404 218 135 3 84 293 25 242 4.27e-26 103
Msa0341490 MtrunA17_Chr1g0146171 36.098 205 111 8 85 277 24 220 5.33e-25 100
Msa0341490 MtrunA17_Chr6g0478191 32.287 223 143 3 84 298 25 247 2.00e-24 99.4
Msa0341490 MtrunA17_Chr2g0326621 31.120 241 132 7 71 278 22 261 1.86e-23 97.4
Msa0341490 MtrunA17_Chr5g0399341 34.783 207 116 4 84 277 23 223 3.89e-23 95.9
Msa0341490 MtrunA17_Chr3g0145671 30.453 243 142 7 73 288 19 261 6.69e-23 95.9
Msa0341490 MtrunA17_Chr4g0030861 33.168 202 127 4 85 278 26 227 1.28e-21 91.7
Msa0341490 MtrunA17_Chr1g0212671 29.075 227 153 3 84 302 25 251 5.66e-21 90.1
Msa0341490 MtrunA17_Chr7g0264111 28.452 239 140 7 71 278 21 259 1.10e-18 84.3
Msa0341490 MtrunA17_Chr7g0264961 33.010 206 121 6 84 277 25 225 1.47e-18 83.2
Msa0341490 MtrunA17_Chr3g0113211 29.508 244 144 8 73 288 37 280 2.84e-18 83.2
Msa0341490 MtrunA17_Chr8g0386961 29.487 234 137 8 73 278 34 267 9.94e-18 81.6
Msa0341490 MtrunA17_Chr4g0006001 29.717 212 131 7 84 278 60 270 1.38e-16 78.6
Msa0341490 MtrunA17_Chr5g0397391 30.189 212 131 6 84 278 56 267 2.02e-16 78.2
Msa0341490 MtrunA17_Chr4g0029071 27.197 239 141 7 73 278 21 259 2.35e-16 77.8
Msa0341490 MtrunA17_Chr7g0222791 27.753 227 156 4 84 302 25 251 9.76e-16 75.5
Msa0341490 MtrunA17_Chr5g0436701 28.692 237 135 8 73 278 37 270 2.74e-15 74.7
Msa0341490 MtrunA17_Chr1g0199731 26.778 239 142 7 73 278 21 259 4.26e-15 74.3
Msa0341490 MtrunA17_Chr4g0020781 29.648 199 130 5 111 303 15 209 1.58e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0341490 AT1G80760.1 77.564 312 58 4 4 310 1 305 6.75e-167 466
Msa0341490 AT4G10380.1 67.416 267 75 3 46 310 48 304 5.13e-119 344
Msa0341490 AT5G37820.1 46.484 256 129 4 52 303 16 267 8.23e-72 224
Msa0341490 AT5G37820.2 46.484 256 129 4 52 303 52 303 1.24e-71 224
Msa0341490 AT4G19030.1 43.686 293 151 5 29 309 2 292 1.56e-70 221
Msa0341490 AT5G37810.1 46.964 247 125 3 59 303 25 267 5.87e-70 219
Msa0341490 AT4G18910.1 41.472 299 146 4 29 309 2 289 2.88e-69 218
Msa0341490 AT1G31885.1 45.600 250 128 2 68 309 31 280 2.60e-66 211
Msa0341490 AT1G31885.2 46.903 226 112 2 92 309 1 226 2.95e-61 196
Msa0341490 AT2G34390.1 40.217 276 148 5 46 310 13 282 1.84e-54 179
Msa0341490 AT2G34390.2 42.083 240 123 4 81 310 7 240 1.53e-51 170
Msa0341490 AT4G19030.3 44.000 225 112 5 29 241 2 224 2.61e-49 164
Msa0341490 AT4G18910.2 42.241 232 105 4 29 242 2 222 2.05e-48 161
Msa0341490 AT3G06100.1 38.208 212 130 1 80 290 46 257 1.50e-43 150
Msa0341490 AT3G06100.2 38.389 211 129 1 84 293 2 212 6.14e-43 147
Msa0341490 AT4G19030.2 41.748 206 106 5 29 222 2 205 1.53e-38 136
Msa0341490 AT2G34390.3 38.942 208 113 4 46 242 13 217 9.26e-35 126
Msa0341490 AT3G16240.1 37.198 207 112 5 84 277 23 224 1.16e-29 113
Msa0341490 AT2G34390.4 37.368 190 105 4 46 224 13 199 8.82e-28 108
Msa0341490 AT2G25810.1 35.885 209 116 7 80 277 19 220 3.40e-26 104
Msa0341490 AT5G47450.1 34.783 230 124 7 84 292 23 247 3.77e-26 104
Msa0341490 AT2G29870.1 46.957 115 58 1 196 310 22 133 1.12e-25 100
Msa0341490 AT4G17340.1 33.816 207 119 4 84 277 23 224 2.63e-24 99.4
Msa0341490 AT3G26520.1 34.783 207 117 6 84 277 26 227 7.96e-23 95.5
Msa0341490 AT2G36830.1 34.783 207 117 6 84 277 25 226 8.65e-23 95.5
Msa0341490 AT4G35100.1 30.864 243 141 7 73 288 20 262 4.12e-22 94.0
Msa0341490 AT4G35100.2 30.864 243 141 7 73 288 20 262 4.12e-22 94.0
Msa0341490 AT2G16850.1 30.328 244 141 7 73 288 18 260 4.74e-22 94.0
Msa0341490 AT5G60660.1 28.689 244 141 7 71 282 21 263 1.73e-20 89.7
Msa0341490 AT3G06100.3 34.532 139 90 1 156 293 41 179 7.77e-20 85.9
Msa0341490 AT3G54820.1 30.833 240 133 7 71 278 20 258 1.93e-19 87.0
Msa0341490 AT1G73190.1 30.493 223 142 4 84 293 28 250 1.43e-18 84.0
Msa0341490 AT4G01470.1 30.275 218 134 5 84 288 25 237 2.32e-18 83.2
Msa0341490 AT1G17810.1 29.956 227 142 6 84 295 28 252 8.86e-18 82.0
Msa0341490 AT2G37170.1 27.308 260 145 8 51 278 10 257 1.36e-17 81.6
Msa0341490 AT3G53420.1 27.917 240 140 7 71 278 21 259 1.62e-17 81.6
Msa0341490 AT3G53420.2 27.917 240 140 7 71 278 21 259 1.62e-17 81.6
Msa0341490 AT2G37170.2 27.308 260 145 8 51 278 53 300 3.02e-17 81.3
Msa0341490 AT2G45960.1 31.915 235 129 9 73 278 34 266 8.17e-17 79.3
Msa0341490 AT2G37180.1 26.923 260 146 8 51 278 10 257 1.09e-16 79.0
Msa0341490 AT4G23400.1 30.189 212 130 6 84 278 57 267 1.32e-16 79.0
Msa0341490 AT4G00430.1 29.487 234 136 8 73 278 35 267 1.45e-16 79.0
Msa0341490 AT1G31885.3 41.758 91 53 0 66 156 29 119 2.05e-16 75.1
Msa0341490 AT1G01620.1 30.660 212 129 6 84 278 56 266 2.45e-16 78.2
Msa0341490 AT2G39010.1 28.333 240 137 7 73 278 20 258 2.56e-16 78.2
Msa0341490 AT3G61430.2 31.455 213 126 7 84 278 56 266 3.38e-16 77.8
Msa0341490 AT3G61430.1 31.455 213 126 7 84 278 56 266 3.38e-16 77.8
Msa0341490 AT3G47440.1 27.586 232 151 5 84 303 27 253 2.35e-15 74.7
Msa0341490 AT1G01620.2 32.402 179 106 5 114 278 17 194 1.41e-14 72.0
Msa0341490 AT2G45960.3 30.594 219 126 7 73 266 34 251 2.09e-14 72.8
Msa0341490 AT2G45960.2 31.818 220 122 8 73 266 34 251 2.58e-14 72.0

Find 160 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAATCATGATTAATTAAT+TGG 0.122870 2_4:-63139328 Msa0341490:five_prime_UTR
TGCACATGGCCAAATGATTT+TGG 0.183955 2_4:+63137174 None:intergenic
TTCATTGACGCTCCTGTTAT+TGG 0.213047 2_4:+63137534 None:intergenic
CATCTTCTAAGTTCCATTCT+TGG 0.216409 2_4:+63138715 None:intergenic
GTGACTCCACCACTCATAAA+AGG 0.246044 2_4:+63138029 None:intergenic
AGTAACTAATTGTTGTTTAC+AGG 0.248708 2_4:+63139400 None:intergenic
CTTACTAGACTCTGGAAATT+AGG 0.255989 2_4:+63139153 None:intergenic
CTACAAACTTGCAGAAATTA+AGG 0.267685 2_4:-63139272 Msa0341490:five_prime_UTR
TCTCCGAGCCTTGTGTCTTT+TGG 0.294166 2_4:+63138413 None:intergenic
CTTTGCATTATCATCTTCTT+CGG 0.298809 2_4:+63137389 None:intergenic
CAGATAGGAGCCGAGTTTAT+AGG 0.306620 2_4:-63138481 Msa0341490:CDS
GAAACTGATATATGAGAAAA+AGG 0.310726 2_4:+63139086 None:intergenic
TGTGCCATTGCTCAAGAATT+AGG 0.311160 2_4:+63139443 None:intergenic
CAGTGTTGGGACTCGTTTGC+GGG 0.317184 2_4:-63136806 Msa0341490:three_prime_UTR
CCAGGAGTGCCTGGTGTTGC+TGG 0.322227 2_4:+63138834 None:intergenic
CCTGGTGTTGCTGGTGTTGA+TGG 0.322832 2_4:+63138843 None:intergenic
CACTAGGCATCAATTATATA+CGG 0.333235 2_4:+63139207 None:intergenic
GTATATTGGTGCACAAGTTT+TGG 0.339591 2_4:-63138093 Msa0341490:CDS
TTTACCATTGACTGTAGAAA+TGG 0.347231 2_4:-63138889 Msa0341490:exon
ATAAGTTATAACACCCTGTT+TGG 0.350832 2_4:-63136631 Msa0341490:three_prime_UTR
AGATAGGAGCCGAGTTTATA+GGG 0.358525 2_4:-63138480 Msa0341490:CDS
TTTGAGGCATGATGCCTGTT+TGG 0.374318 2_4:-63136682 Msa0341490:three_prime_UTR
TTTCAAAGTAAAGTTATTGA+TGG 0.375157 2_4:-63136714 Msa0341490:three_prime_UTR
GCTGGACCTAGAGTTCTTAC+TGG 0.377381 2_4:+63137510 None:intergenic
CTGCGTTGCAGGCCAATAAC+AGG 0.388557 2_4:-63137546 Msa0341490:intron
CTCTCCACCGGCCACATCTC+TGG 0.392505 2_4:-63138340 Msa0341490:CDS
ACCGTTCCTTCCGGAGGATA+TGG 0.393092 2_4:-63138008 Msa0341490:CDS
GAGTTGAACTTACGTCTTTG+TGG 0.400319 2_4:+63136832 None:intergenic
TAGTTATTTGCAGCTATAGC+TGG 0.401428 2_4:+63137492 None:intergenic
ATTGGGTGTGCTACCTCTAC+TGG 0.401500 2_4:-63138385 Msa0341490:CDS
CAAATAACTACAAAGCCATA+TGG 0.404031 2_4:-63137479 Msa0341490:CDS
ACATTGATTCTGATGTTTGC+TGG 0.409739 2_4:-63138457 Msa0341490:CDS
AATTGAGTGCTCTTTCAGTT+TGG 0.411453 2_4:-63137075 Msa0341490:three_prime_UTR
AATTTACATTAGACAAGAAA+AGG 0.411623 2_4:+63138910 None:intergenic
CAAGGCTCGGAGACTCTGAT+TGG 0.413496 2_4:-63138403 Msa0341490:CDS
TATTGTTGCTATTGTATGTC+GGG 0.414071 2_4:+63138969 None:intergenic
TTTGCATTATCATCTTCTTC+GGG 0.414167 2_4:+63137390 None:intergenic
TGCAAGAGGCACAGGAGGAG+GGG 0.422658 2_4:+63138656 None:intergenic
TTTCCATTCATCAATTTCCT+TGG 0.427402 2_4:+63139231 None:intergenic
CATTGATTCTGATGTTTGCT+GGG 0.434089 2_4:-63138456 Msa0341490:CDS
CTCAAGAATTGTAAATGCTT+CGG 0.434143 2_4:-63138741 Msa0341490:CDS
TGTTCCATTTCTACAGTCAA+TGG 0.435070 2_4:+63138885 None:intergenic
CCACCTAGAGCTCCAAGTAT+TGG 0.435294 2_4:+63137441 None:intergenic
GGTGGAGTCACCGTTCCTTC+CGG 0.437337 2_4:-63138017 Msa0341490:CDS
ATTGGCAACAGATAAAATAT+TGG 0.440460 2_4:-63136938 Msa0341490:three_prime_UTR
GTGCTGCATTTGCTCTCAAA+GGG 0.443470 2_4:-63138061 Msa0341490:CDS
AGGCATGATGCCTGTTTGGA+TGG 0.444544 2_4:-63136678 Msa0341490:three_prime_UTR
TTTGCAAGAGGCACAGGAGG+AGG 0.446500 2_4:+63138654 None:intergenic
TATCTCTTAGCTCCAATACT+TGG 0.455159 2_4:-63137453 Msa0341490:CDS
TAGCATGACTAGTATCACTA+TGG 0.456539 2_4:+63139037 None:intergenic
AGTTGATCATCTTCTAAAGC+TGG 0.459544 2_4:+63137344 None:intergenic
TCAAATCAGCCCATCCAAAC+AGG 0.460558 2_4:+63136668 None:intergenic
TTGCAGGTGCCTATGTATAT+TGG 0.461576 2_4:-63138107 Msa0341490:intron
AATACACTCTCATGATACTT+AGG 0.463385 2_4:-63139357 Msa0341490:five_prime_UTR
TTAATTATCTCTAATTACAT+AGG 0.466666 2_4:-63136969 Msa0341490:three_prime_UTR
CTTCAATGCAGCAAAAGAAA+TGG 0.468532 2_4:+63138294 None:intergenic
AGTTGAACTTACGTCTTTGT+GGG 0.469754 2_4:+63136833 None:intergenic
TTGATGGGCAATCTGCTTTG+AGG 0.471425 2_4:-63136698 Msa0341490:three_prime_UTR
ATGCTCAATATACTAATAGC+AGG 0.472157 2_4:-63137665 Msa0341490:CDS
TTGCAAGAGGCACAGGAGGA+GGG 0.478192 2_4:+63138655 None:intergenic
AGAGGTACACCAGGAGTGCC+TGG 0.482854 2_4:+63138825 None:intergenic
TAAGTTATAACACCCTGTTT+GGG 0.487316 2_4:-63136630 Msa0341490:three_prime_UTR
AAATAACTACAAAGCCATAT+GGG 0.491686 2_4:-63137478 Msa0341490:CDS
TTCAAAGTAAAGTTATTGAT+GGG 0.492195 2_4:-63136713 Msa0341490:three_prime_UTR
AATGCTTCGGTGTCCAAGAA+TGG 0.493128 2_4:-63138728 Msa0341490:CDS
AACTCTTGATTAGAAAGAGC+TGG 0.493233 2_4:-63136891 Msa0341490:three_prime_UTR
GAAATTAAGGAGAACACAAA+AGG 0.495825 2_4:-63139259 Msa0341490:five_prime_UTR
CTTGGAGCTCTAGGTGGGGC+AGG 0.496173 2_4:-63137435 Msa0341490:CDS
GCAAGAGGCACAGGAGGAGG+GGG 0.497170 2_4:+63138657 None:intergenic
GCATTTACAATTCTTGAGTA+GGG 0.497185 2_4:+63138746 None:intergenic
GCAAAAGAAATGGTGACTGC+AGG 0.502542 2_4:+63138304 None:intergenic
AATGAAAGGAAGCAAATTAG+TGG 0.504370 2_4:+63139061 None:intergenic
CCAGTGTTGGGACTCGTTTG+CGG 0.510183 2_4:-63136807 Msa0341490:three_prime_UTR
TTCATAAGCTATCTTGGAGA+AGG 0.510833 2_4:+63136548 None:intergenic
AGTTCAACTCTGCCAGTGTT+GGG 0.512112 2_4:-63136819 Msa0341490:three_prime_UTR
CAAGAATGGAACTTAGAAGA+TGG 0.514046 2_4:-63138714 Msa0341490:CDS
CCAGCAACACCAGGCACTCC+TGG 0.515961 2_4:-63138834 Msa0341490:CDS
GTTGATCATCTTCTAAAGCT+GGG 0.517734 2_4:+63137345 None:intergenic
TCATCATAAAATGACACTAA+AGG 0.518324 2_4:-63137298 Msa0341490:three_prime_UTR
AATGGAACATATTAATAATG+AGG 0.519868 2_4:-63138871 Msa0341490:CDS
ATGAACCCAGTAAGAACTCT+AGG 0.522426 2_4:-63137516 Msa0341490:CDS
TGATCCTTGAGGCTTATTGA+AGG 0.522724 2_4:-63136773 Msa0341490:three_prime_UTR
TAATCTAATGTTCGTTGTCA+CGG 0.524885 2_4:-63137952 Msa0341490:CDS
AAGGTAGCAGCATGTGGAAG+TGG 0.525892 2_4:+63139105 None:intergenic
GACTCTGGAAATTAGGAAAG+AGG 0.528630 2_4:+63139160 None:intergenic
TTATCCTAATTCTTGAGCAA+TGG 0.528782 2_4:-63139447 Msa0341490:five_prime_UTR
TGACCAAGGAAATTGATGAA+TGG 0.530797 2_4:-63139234 Msa0341490:five_prime_UTR
TTATTGTTGCTATTGTATGT+CGG 0.531372 2_4:+63138968 None:intergenic
GGCATGATGCCTGTTTGGAT+GGG 0.531642 2_4:-63136677 Msa0341490:three_prime_UTR
GCTCCAATACTTGGAGCTCT+AGG 0.532633 2_4:-63137444 Msa0341490:CDS
AGCATTTACAATTCTTGAGT+AGG 0.536216 2_4:+63138745 None:intergenic
TGACTCCACCACTCATAAAA+GGG 0.537986 2_4:+63138030 None:intergenic
GCTTTGGTTACATGCTTCCA+CGG 0.548483 2_4:+63138265 None:intergenic
TTATGATGATTGATGAGCAT+GGG 0.550009 2_4:+63137312 None:intergenic
TGTGCTGCATTTGCTCTCAA+AGG 0.550088 2_4:-63138062 Msa0341490:CDS
GTATATCTAATTCGTGCACA+TGG 0.551064 2_4:+63137160 None:intergenic
AAGGCTCGGAGACTCTGATT+GGG 0.552417 2_4:-63138402 Msa0341490:CDS
TTTATGATGATTGATGAGCA+TGG 0.552805 2_4:+63137311 None:intergenic
GGTTTAAGTCTGAGAGAAGC+GGG 0.553612 2_4:-63138794 Msa0341490:CDS
ATTTACATTAGACAAGAAAA+GGG 0.554099 2_4:+63138911 None:intergenic
AATGTTCGTTGTCACGGCAG+TGG 0.554743 2_4:-63137946 Msa0341490:CDS
AAGTCTGAGAGAAGCGGGAA+TGG 0.555069 2_4:-63138789 Msa0341490:CDS
AGGGGACTCTTACTAGACTC+TGG 0.557413 2_4:+63139145 None:intergenic
CAGAGATGTGGCCGGTGGAG+AGG 0.557528 2_4:+63138341 None:intergenic
CTGGACCTAGAGTTCTTACT+GGG 0.557635 2_4:+63137511 None:intergenic
TGCTACCTCTACTGGTCTTG+CGG 0.560160 2_4:-63138377 Msa0341490:CDS
ATTACATAGGAAATAACCAT+TGG 0.560431 2_4:-63136956 Msa0341490:three_prime_UTR
CAATACTTGGAGCTCTAGGT+GGG 0.562103 2_4:-63137440 Msa0341490:CDS
AAATTAGAGTACAGAAGCAC+GGG 0.563522 2_4:+63138938 None:intergenic
GTAACTAATTGTTGTTTACA+GGG 0.565538 2_4:+63139401 None:intergenic
GGACGGTGGGACAGTTCTGA+GGG 0.568449 2_4:+63139126 None:intergenic
AAGTTCAACTCTGCCAGTGT+TGG 0.569468 2_4:-63136820 Msa0341490:three_prime_UTR
GAACTAGCAGGAATTGCAGT+GGG 0.569469 2_4:-63137698 Msa0341490:CDS
CCAATACTTGGAGCTCTAGG+TGG 0.572700 2_4:-63137441 Msa0341490:CDS
ACCATATCCTCCGGAAGGAA+CGG 0.576185 2_4:+63138007 None:intergenic
AGCTAAGAGATAGACCCATA+TGG 0.576739 2_4:+63137464 None:intergenic
CCATCAACACCAGCAACACC+AGG 0.577155 2_4:-63138843 Msa0341490:CDS
TGGACGGTGGGACAGTTCTG+AGG 0.577190 2_4:+63139125 None:intergenic
CTGCATTGAAGCACTTCCCG+TGG 0.578067 2_4:-63138282 Msa0341490:CDS
CTTCACTCACTTAAAGAGCA+TGG 0.578332 2_4:+63139305 None:intergenic
TGGAAATTAGGAAAGAGGCT+TGG 0.583275 2_4:+63139165 None:intergenic
AGTATCACTATGGAAATGAA+AGG 0.584129 2_4:+63139047 None:intergenic
CATAACCGCAAGACCAGTAG+AGG 0.586587 2_4:+63138372 None:intergenic
CATTAATGAGCAGGTAACTG+TGG 0.587885 2_4:+63138683 None:intergenic
ATGATCATCATCCTCTCCAC+CGG 0.588983 2_4:-63138352 Msa0341490:CDS
CAAAAGAAATGGTGACTGCA+GGG 0.592708 2_4:+63138305 None:intergenic
AAAAGCTTGACCATATCCTC+CGG 0.599263 2_4:+63137998 None:intergenic
ACTTGTGCACCAATATACAT+AGG 0.600697 2_4:+63138098 None:intergenic
ACACTACACTACACTACACT+AGG 0.601752 2_4:+63139191 None:intergenic
AATCAATGTCCCTATAAACT+CGG 0.603006 2_4:+63138471 None:intergenic
GAAATTAGAGTACAGAAGCA+CGG 0.609449 2_4:+63138937 None:intergenic
CTTTGGTTACATGCTTCCAC+GGG 0.610743 2_4:+63138266 None:intergenic
AGCACCAGAGATGTGGCCGG+TGG 0.615438 2_4:+63138336 None:intergenic
TGAGATGAGCACCAGAGATG+TGG 0.617163 2_4:+63138329 None:intergenic
TGCTGCATTTGCTCTCAAAG+GGG 0.620449 2_4:-63138060 Msa0341490:CDS
GGAGTCACCGTTCCTTCCGG+AGG 0.622617 2_4:-63138014 Msa0341490:CDS
ACTCACAGCTCTTGTGTCGG+TGG 0.623869 2_4:+63137923 None:intergenic
CCGCAAACGAGTCCCAACAC+TGG 0.624277 2_4:+63136807 None:intergenic
GGGTTTAAGTCTGAGAGAAG+CGG 0.625558 2_4:-63138795 Msa0341490:CDS
ATGTAGGTAGGAGAACTAGC+AGG 0.625936 2_4:-63137710 Msa0341490:intron
ATTAATGAGCAGGTAACTGT+GGG 0.626119 2_4:+63138684 None:intergenic
TAGCAGCATGTGGAAGTGGA+CGG 0.626553 2_4:+63139109 None:intergenic
AGAACTAGCAGGAATTGCAG+TGG 0.628860 2_4:-63137699 Msa0341490:CDS
CATGCTCTTTAAGTGAGTGA+AGG 0.634040 2_4:-63139304 Msa0341490:five_prime_UTR
GTTGAACTTACGTCTTTGTG+GGG 0.634464 2_4:+63136834 None:intergenic
AGCATGTGGAAGTGGACGGT+GGG 0.635263 2_4:+63139113 None:intergenic
GCAATCTGCTATGATCCTTG+AGG 0.641389 2_4:-63136784 Msa0341490:three_prime_UTR
TTGATCATCTTCTAAAGCTG+GGG 0.642052 2_4:+63137346 None:intergenic
GAACCAAAAGACACAAGGCT+CGG 0.650309 2_4:-63138416 Msa0341490:CDS
GCTTGACCATATCCTCCGGA+AGG 0.651188 2_4:+63138002 None:intergenic
TATGATGATTGATGAGCATG+GGG 0.652693 2_4:+63137313 None:intergenic
CAGCATGTGGAAGTGGACGG+TGG 0.655771 2_4:+63139112 None:intergenic
AATACTTGGAGCTCTAGGTG+GGG 0.656773 2_4:-63137439 Msa0341490:CDS
TGCTCAATATACTAATAGCA+GGG 0.657754 2_4:-63137664 Msa0341490:intron
GAACACAAAAGGAATGACCA+AGG 0.662028 2_4:-63139248 Msa0341490:five_prime_UTR
AATACTCACAGCTCTTGTGT+CGG 0.671145 2_4:+63137920 None:intergenic
ATGAGCACCAGAGATGTGGC+CGG 0.675762 2_4:+63138333 None:intergenic
ATTGTGAACCAAAAGACACA+AGG 0.703696 2_4:-63138421 Msa0341490:CDS
GACGGTGGGACAGTTCTGAG+GGG 0.726625 2_4:+63139127 None:intergenic
ATGATGATTGATGAGCATGG+GGG 0.825649 2_4:+63137314 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATCATGATTAATTAAT+TGG - chr2_4:63136693-63136712 Msa0341490:three_prime_UTR 10.0%
!! TAATTTCAACAACTTTAATT+AGG + chr2_4:63137808-63137827 None:intergenic 15.0%
!! AAAGTATAAATTATAATTGC+AGG - chr2_4:63137898-63137917 Msa0341490:intron 15.0%
!!! CATATGTTCAAATTTTAATT+TGG + chr2_4:63138226-63138245 None:intergenic 15.0%
!!! ATATATATATAAGTTTTGTG+TGG - chr2_4:63138812-63138831 Msa0341490:CDS 15.0%
!! TTAATTATCTCTAATTACAT+AGG - chr2_4:63139052-63139071 Msa0341490:five_prime_UTR 15.0%
!! ATTTACATTAGACAAGAAAA+GGG + chr2_4:63137113-63137132 None:intergenic 20.0%
!! AATTTACATTAGACAAGAAA+AGG + chr2_4:63137114-63137133 None:intergenic 20.0%
!! AATGGAACATATTAATAATG+AGG - chr2_4:63137150-63137169 Msa0341490:three_prime_UTR 20.0%
!! ACAACTTTAATTAGGAAATT+AGG + chr2_4:63137800-63137819 None:intergenic 20.0%
!! TATTAGCTAAAAGTTACTAT+AGG + chr2_4:63138131-63138150 None:intergenic 20.0%
!! TTAAAATTTGAACATATGCT+TGG - chr2_4:63138228-63138247 Msa0341490:intron 20.0%
!! ATAACCATAAACATCAATTA+GGG + chr2_4:63138293-63138312 None:intergenic 20.0%
!! TTTCAAAGTAAAGTTATTGA+TGG - chr2_4:63139307-63139326 Msa0341490:five_prime_UTR 20.0%
!! TTCAAAGTAAAGTTATTGAT+GGG - chr2_4:63139308-63139327 Msa0341490:five_prime_UTR 20.0%
!!! GTAACTAATTGTTGTTTACA+GGG + chr2_4:63136623-63136642 None:intergenic 25.0%
!!! AGTAACTAATTGTTGTTTAC+AGG + chr2_4:63136624-63136643 None:intergenic 25.0%
! GAAACTGATATATGAGAAAA+AGG + chr2_4:63136938-63136957 None:intergenic 25.0%
!! TTATTGTTGCTATTGTATGT+CGG + chr2_4:63137056-63137075 None:intergenic 25.0%
!!! ATATATACCTTTTTTGCAAG+AGG + chr2_4:63137382-63137401 None:intergenic 25.0%
! ATTTATGTGTGTCTAATCTT+TGG - chr2_4:63137440-63137459 Msa0341490:CDS 25.0%
! AGTTTGAAAAGAAAAAGAGA+AGG - chr2_4:63138169-63138188 Msa0341490:intron 25.0%
! CATAACCATAAACATCAATT+AGG + chr2_4:63138294-63138313 None:intergenic 25.0%
! ATTGATGTTTATGGTTATGT+AGG - chr2_4:63138295-63138314 Msa0341490:CDS 25.0%
! TATATATGTTCATGACAAGT+GGG + chr2_4:63138445-63138464 None:intergenic 25.0%
! ATATATATGTTCATGACAAG+TGG + chr2_4:63138446-63138465 None:intergenic 25.0%
! TGTCATGAACATATATATGA+TGG - chr2_4:63138448-63138467 Msa0341490:CDS 25.0%
! AAATAACTACAAAGCCATAT+GGG - chr2_4:63138543-63138562 Msa0341490:intron 25.0%
! TCATCATAAAATGACACTAA+AGG - chr2_4:63138723-63138742 Msa0341490:CDS 25.0%
! AAAAACAAGCCAAAATCATT+TGG - chr2_4:63138838-63138857 Msa0341490:CDS 25.0%
!! TCTTTTACTTTAACCATGTA+AGG + chr2_4:63139009-63139028 None:intergenic 25.0%
! ATTACATAGGAAATAACCAT+TGG - chr2_4:63139065-63139084 Msa0341490:five_prime_UTR 25.0%
!! AATATTTTATCTGTTGCCAA+TGG + chr2_4:63139084-63139103 None:intergenic 25.0%
! ATTGGCAACAGATAAAATAT+TGG - chr2_4:63139083-63139102 Msa0341490:five_prime_UTR 25.0%
! GCTTATTGAAGGAAAAAAAA+TGG - chr2_4:63139259-63139278 Msa0341490:five_prime_UTR 25.0%
TTATCCTAATTCTTGAGCAA+TGG - chr2_4:63136574-63136593 Msa0341490:three_prime_UTR 30.0%
AATACACTCTCATGATACTT+AGG - chr2_4:63136664-63136683 Msa0341490:three_prime_UTR 30.0%
CTACAAACTTGCAGAAATTA+AGG - chr2_4:63136749-63136768 Msa0341490:three_prime_UTR 30.0%
GAAATTAAGGAGAACACAAA+AGG - chr2_4:63136762-63136781 Msa0341490:three_prime_UTR 30.0%
TTTCCATTCATCAATTTCCT+TGG + chr2_4:63136793-63136812 None:intergenic 30.0%
CACTAGGCATCAATTATATA+CGG + chr2_4:63136817-63136836 None:intergenic 30.0%
AATGAAAGGAAGCAAATTAG+TGG + chr2_4:63136963-63136982 None:intergenic 30.0%
AGTATCACTATGGAAATGAA+AGG + chr2_4:63136977-63136996 None:intergenic 30.0%
! TATTGTTGCTATTGTATGTC+GGG + chr2_4:63137055-63137074 None:intergenic 30.0%
! TTTACCATTGACTGTAGAAA+TGG - chr2_4:63137132-63137151 Msa0341490:three_prime_UTR 30.0%
GCATTTACAATTCTTGAGTA+GGG + chr2_4:63137278-63137297 None:intergenic 30.0%
AGCATTTACAATTCTTGAGT+AGG + chr2_4:63137279-63137298 None:intergenic 30.0%
CTCAAGAATTGTAAATGCTT+CGG - chr2_4:63137280-63137299 Msa0341490:intron 30.0%
TACTTAGCACTTCAATGATA+TGG + chr2_4:63137486-63137505 None:intergenic 30.0%
AATCAATGTCCCTATAAACT+CGG + chr2_4:63137553-63137572 None:intergenic 30.0%
TAATTAGGAAATTAGGCTGA+AGG + chr2_4:63137793-63137812 None:intergenic 30.0%
TAATCTAATGTTCGTTGTCA+CGG - chr2_4:63138069-63138088 Msa0341490:CDS 30.0%
ATGTTTATGGTTATGTAGGT+AGG - chr2_4:63138299-63138318 Msa0341490:CDS 30.0%
ATGCTCAATATACTAATAGC+AGG - chr2_4:63138356-63138375 Msa0341490:CDS 30.0%
TGCTCAATATACTAATAGCA+GGG - chr2_4:63138357-63138376 Msa0341490:CDS 30.0%
ATATATGTTCATGACAAGTG+GGG + chr2_4:63138444-63138463 None:intergenic 30.0%
CAAATAACTACAAAGCCATA+TGG - chr2_4:63138542-63138561 Msa0341490:intron 30.0%
TTTGCATTATCATCTTCTTC+GGG + chr2_4:63138634-63138653 None:intergenic 30.0%
CTTTGCATTATCATCTTCTT+CGG + chr2_4:63138635-63138654 None:intergenic 30.0%
TTATGATGATTGATGAGCAT+GGG + chr2_4:63138712-63138731 None:intergenic 30.0%
TTTATGATGATTGATGAGCA+TGG + chr2_4:63138713-63138732 None:intergenic 30.0%
! CTATTTTCTTTCTCCTTACA+TGG - chr2_4:63138993-63139012 Msa0341490:five_prime_UTR 30.0%
ATAAGTTATAACACCCTGTT+TGG - chr2_4:63139390-63139409 Msa0341490:five_prime_UTR 30.0%
TAAGTTATAACACCCTGTTT+GGG - chr2_4:63139391-63139410 Msa0341490:five_prime_UTR 30.0%
AGCTAATGAAAAATGTCACA+AGG + chr2_4:63139433-63139452 None:intergenic 30.0%
TGACCAAGGAAATTGATGAA+TGG - chr2_4:63136787-63136806 Msa0341490:three_prime_UTR 35.0%
! CTTACTAGACTCTGGAAATT+AGG + chr2_4:63136871-63136890 None:intergenic 35.0%
TAGCATGACTAGTATCACTA+TGG + chr2_4:63136987-63137006 None:intergenic 35.0%
! AAATTAGAGTACAGAAGCAC+GGG + chr2_4:63137086-63137105 None:intergenic 35.0%
GAAATTAGAGTACAGAAGCA+CGG + chr2_4:63137087-63137106 None:intergenic 35.0%
TGTTCCATTTCTACAGTCAA+TGG + chr2_4:63137139-63137158 None:intergenic 35.0%
CATCTTCTAAGTTCCATTCT+TGG + chr2_4:63137309-63137328 None:intergenic 35.0%
CAAGAATGGAACTTAGAAGA+TGG - chr2_4:63137307-63137326 Msa0341490:three_prime_UTR 35.0%
ATTAATGAGCAGGTAACTGT+GGG + chr2_4:63137340-63137359 None:intergenic 35.0%
! ATCTGTAACACAGGTAGTTT+TGG + chr2_4:63137525-63137544 None:intergenic 35.0%
AAACTACCTGTGTTACAGAT+AGG - chr2_4:63137525-63137544 Msa0341490:CDS 35.0%
!! ACATTGATTCTGATGTTTGC+TGG - chr2_4:63137564-63137583 Msa0341490:intron 35.0%
!! CATTGATTCTGATGTTTGCT+GGG - chr2_4:63137565-63137584 Msa0341490:intron 35.0%
ATTGTGAACCAAAAGACACA+AGG - chr2_4:63137600-63137619 Msa0341490:intron 35.0%
CTTCAATGCAGCAAAAGAAA+TGG + chr2_4:63137730-63137749 None:intergenic 35.0%
GAAGGATTAAGGAAAAGCTT+TGG + chr2_4:63137775-63137794 None:intergenic 35.0%
GAAATTAGGCTGAAGGATTA+AGG + chr2_4:63137786-63137805 None:intergenic 35.0%
ACTTGTGCACCAATATACAT+AGG + chr2_4:63137926-63137945 None:intergenic 35.0%
! GTATATTGGTGCACAAGTTT+TGG - chr2_4:63137928-63137947 Msa0341490:intron 35.0%
! GTTTTTCACCCTTTTATGAG+TGG - chr2_4:63137983-63138002 Msa0341490:CDS 35.0%
GATGATATTATCCCCTATGT+CGG + chr2_4:63138204-63138223 None:intergenic 35.0%
GAGTCCCTAATTGATGTTTA+TGG - chr2_4:63138286-63138305 Msa0341490:CDS 35.0%
TAGTTATTTGCAGCTATAGC+TGG + chr2_4:63138532-63138551 None:intergenic 35.0%
TATCTCTTAGCTCCAATACT+TGG - chr2_4:63138568-63138587 Msa0341490:intron 35.0%
!! TTGATCATCTTCTAAAGCTG+GGG + chr2_4:63138678-63138697 None:intergenic 35.0%
!! GTTGATCATCTTCTAAAGCT+GGG + chr2_4:63138679-63138698 None:intergenic 35.0%
!! AGTTGATCATCTTCTAAAGC+TGG + chr2_4:63138680-63138699 None:intergenic 35.0%
TATGATGATTGATGAGCATG+GGG + chr2_4:63138711-63138730 None:intergenic 35.0%
GTATATCTAATTCGTGCACA+TGG + chr2_4:63138864-63138883 None:intergenic 35.0%
AATTGAGTGCTCTTTCAGTT+TGG - chr2_4:63138946-63138965 Msa0341490:five_prime_UTR 35.0%
AACTCTTGATTAGAAAGAGC+TGG - chr2_4:63139130-63139149 Msa0341490:five_prime_UTR 35.0%
AGTTGAACTTACGTCTTTGT+GGG + chr2_4:63139191-63139210 None:intergenic 35.0%
! TTTTCCTTCAATAAGCCTCA+AGG + chr2_4:63139255-63139274 None:intergenic 35.0%
! TTCACAAGTTTTCCCAAACA+GGG + chr2_4:63139406-63139425 None:intergenic 35.0%
! TTTCACAAGTTTTCCCAAAC+AGG + chr2_4:63139407-63139426 None:intergenic 35.0%
TTCATAAGCTATCTTGGAGA+AGG + chr2_4:63139476-63139495 None:intergenic 35.0%
! TGTGCCATTGCTCAAGAATT+AGG + chr2_4:63136581-63136600 None:intergenic 40.0%
CTTCACTCACTTAAAGAGCA+TGG + chr2_4:63136719-63136738 None:intergenic 40.0%
CATGCTCTTTAAGTGAGTGA+AGG - chr2_4:63136717-63136736 Msa0341490:three_prime_UTR 40.0%
GAACACAAAAGGAATGACCA+AGG - chr2_4:63136773-63136792 Msa0341490:three_prime_UTR 40.0%
ACACTACACTACACTACACT+AGG + chr2_4:63136833-63136852 None:intergenic 40.0%
TGGAAATTAGGAAAGAGGCT+TGG + chr2_4:63136859-63136878 None:intergenic 40.0%
! GACTCTGGAAATTAGGAAAG+AGG + chr2_4:63136864-63136883 None:intergenic 40.0%
GACTTAAACCCACCAAAAAG+AGG + chr2_4:63137217-63137236 None:intergenic 40.0%
CATTAATGAGCAGGTAACTG+TGG + chr2_4:63137341-63137360 None:intergenic 40.0%
AGATAGGAGCCGAGTTTATA+GGG - chr2_4:63137541-63137560 Msa0341490:intron 40.0%
!!! CTTTTGGTTCACAATAGCAG+TGG + chr2_4:63137595-63137614 None:intergenic 40.0%
CAAAAGAAATGGTGACTGCA+GGG + chr2_4:63137719-63137738 None:intergenic 40.0%
TTGCAGGTGCCTATGTATAT+TGG - chr2_4:63137914-63137933 Msa0341490:intron 40.0%
! TTTCACCCTTTTATGAGTGG+TGG - chr2_4:63137986-63138005 Msa0341490:CDS 40.0%
TGACTCCACCACTCATAAAA+GGG + chr2_4:63137994-63138013 None:intergenic 40.0%
AAAAGCTTGACCATATCCTC+CGG + chr2_4:63138026-63138045 None:intergenic 40.0%
AATACTCACAGCTCTTGTGT+CGG + chr2_4:63138104-63138123 None:intergenic 40.0%
TTAATTGCTCTTCACGCGAA+GGG + chr2_4:63138264-63138283 None:intergenic 40.0%
GAGAGTGATAAGTGAGAGTT+AGG + chr2_4:63138394-63138413 None:intergenic 40.0%
TTCATTGACGCTCCTGTTAT+TGG + chr2_4:63138490-63138509 None:intergenic 40.0%
ATGAACCCAGTAAGAACTCT+AGG - chr2_4:63138505-63138524 Msa0341490:intron 40.0%
AGCTAAGAGATAGACCCATA+TGG + chr2_4:63138560-63138579 None:intergenic 40.0%
ATGATGATTGATGAGCATGG+GGG + chr2_4:63138710-63138729 None:intergenic 40.0%
! TGCACATGGCCAAATGATTT+TGG + chr2_4:63138850-63138869 None:intergenic 40.0%
! AGCACTCAATTTTGCTTGCT+TGG + chr2_4:63138937-63138956 None:intergenic 40.0%
GTTGAACTTACGTCTTTGTG+GGG + chr2_4:63139190-63139209 None:intergenic 40.0%
GAGTTGAACTTACGTCTTTG+TGG + chr2_4:63139192-63139211 None:intergenic 40.0%
TGATCCTTGAGGCTTATTGA+AGG - chr2_4:63139248-63139267 Msa0341490:five_prime_UTR 40.0%
GAGAAAAAGGTAGCAGCATG+TGG + chr2_4:63136925-63136944 None:intergenic 45.0%
! ACTCCTGGTGTACCTCTTTT+TGG - chr2_4:63137202-63137221 Msa0341490:three_prime_UTR 45.0%
!! CTGGTGTACCTCTTTTTGGT+GGG - chr2_4:63137206-63137225 Msa0341490:three_prime_UTR 45.0%
GGGTTTAAGTCTGAGAGAAG+CGG - chr2_4:63137226-63137245 Msa0341490:intron 45.0%
GGTTTAAGTCTGAGAGAAGC+GGG - chr2_4:63137227-63137246 Msa0341490:intron 45.0%
!! AATGCTTCGGTGTCCAAGAA+TGG - chr2_4:63137293-63137312 Msa0341490:three_prime_UTR 45.0%
!!! TTTTTTGCAAGAGGCACAGG+AGG + chr2_4:63137373-63137392 None:intergenic 45.0%
TCCTGTGCCTCTTGCAAAAA+AGG - chr2_4:63137372-63137391 Msa0341490:CDS 45.0%
!!! ACCTTTTTTGCAAGAGGCAC+AGG + chr2_4:63137376-63137395 None:intergenic 45.0%
CAGATAGGAGCCGAGTTTAT+AGG - chr2_4:63137540-63137559 Msa0341490:intron 45.0%
GAACCAAAAGACACAAGGCT+CGG - chr2_4:63137605-63137624 Msa0341490:intron 45.0%
ATGATCATCATCCTCTCCAC+CGG - chr2_4:63137669-63137688 Msa0341490:CDS 45.0%
GCAAAAGAAATGGTGACTGC+AGG + chr2_4:63137720-63137739 None:intergenic 45.0%
CTTTGGTTACATGCTTCCAC+GGG + chr2_4:63137758-63137777 None:intergenic 45.0%
GCTTTGGTTACATGCTTCCA+CGG + chr2_4:63137759-63137778 None:intergenic 45.0%
TGTGCTGCATTTGCTCTCAA+AGG - chr2_4:63137959-63137978 Msa0341490:CDS 45.0%
GTGCTGCATTTGCTCTCAAA+GGG - chr2_4:63137960-63137979 Msa0341490:CDS 45.0%
TGCTGCATTTGCTCTCAAAG+GGG - chr2_4:63137961-63137980 Msa0341490:CDS 45.0%
GTGACTCCACCACTCATAAA+AGG + chr2_4:63137995-63138014 None:intergenic 45.0%
AAGGAAGATGAAGCCGACAT+AGG - chr2_4:63138188-63138207 Msa0341490:intron 45.0%
AGGAAGATGAAGCCGACATA+GGG - chr2_4:63138189-63138208 Msa0341490:intron 45.0%
TAATTGCTCTTCACGCGAAG+GGG + chr2_4:63138263-63138282 None:intergenic 45.0%
GTTAATTGCTCTTCACGCGA+AGG + chr2_4:63138265-63138284 None:intergenic 45.0%
ATGTAGGTAGGAGAACTAGC+AGG - chr2_4:63138311-63138330 Msa0341490:CDS 45.0%
AGAACTAGCAGGAATTGCAG+TGG - chr2_4:63138322-63138341 Msa0341490:CDS 45.0%
GAACTAGCAGGAATTGCAGT+GGG - chr2_4:63138323-63138342 Msa0341490:CDS 45.0%
TTCATGACAAGTGGGGATAG+GGG + chr2_4:63138437-63138456 None:intergenic 45.0%
GTTCATGACAAGTGGGGATA+GGG + chr2_4:63138438-63138457 None:intergenic 45.0%
TGTTCATGACAAGTGGGGAT+AGG + chr2_4:63138439-63138458 None:intergenic 45.0%
CTGGACCTAGAGTTCTTACT+GGG + chr2_4:63138513-63138532 None:intergenic 45.0%
!! CAATACTTGGAGCTCTAGGT+GGG - chr2_4:63138581-63138600 Msa0341490:intron 45.0%
!! AATACTTGGAGCTCTAGGTG+GGG - chr2_4:63138582-63138601 Msa0341490:intron 45.0%
AAGTTCAACTCTGCCAGTGT+TGG - chr2_4:63139201-63139220 Msa0341490:five_prime_UTR 45.0%
AGTTCAACTCTGCCAGTGTT+GGG - chr2_4:63139202-63139221 Msa0341490:five_prime_UTR 45.0%
GCAATCTGCTATGATCCTTG+AGG - chr2_4:63139237-63139256 Msa0341490:five_prime_UTR 45.0%
! TTGATGGGCAATCTGCTTTG+AGG - chr2_4:63139323-63139342 Msa0341490:five_prime_UTR 45.0%
! TTTGAGGCATGATGCCTGTT+TGG - chr2_4:63139339-63139358 Msa0341490:five_prime_UTR 45.0%
TCAAATCAGCCCATCCAAAC+AGG + chr2_4:63139356-63139375 None:intergenic 45.0%
AGGGGACTCTTACTAGACTC+TGG + chr2_4:63136879-63136898 None:intergenic 50.0%
TAGCAGCATGTGGAAGTGGA+CGG + chr2_4:63136915-63136934 None:intergenic 50.0%
AAGGTAGCAGCATGTGGAAG+TGG + chr2_4:63136919-63136938 None:intergenic 50.0%
! CCACCAAAAAGAGGTACACC+AGG + chr2_4:63137208-63137227 None:intergenic 50.0%
! CCTGGTGTACCTCTTTTTGG+TGG - chr2_4:63137205-63137224 Msa0341490:three_prime_UTR 50.0%
AAGTCTGAGAGAAGCGGGAA+TGG - chr2_4:63137232-63137251 Msa0341490:intron 50.0%
TCGGCTCCTATCTGTAACAC+AGG + chr2_4:63137534-63137553 None:intergenic 50.0%
!! TCTCCGAGCCTTGTGTCTTT+TGG + chr2_4:63137611-63137630 None:intergenic 50.0%
AAGGCTCGGAGACTCTGATT+GGG - chr2_4:63137619-63137638 Msa0341490:intron 50.0%
ATTGGGTGTGCTACCTCTAC+TGG - chr2_4:63137636-63137655 Msa0341490:intron 50.0%
!! TGCTACCTCTACTGGTCTTG+CGG - chr2_4:63137644-63137663 Msa0341490:intron 50.0%
CATAACCGCAAGACCAGTAG+AGG + chr2_4:63137652-63137671 None:intergenic 50.0%
TGAGATGAGCACCAGAGATG+TGG + chr2_4:63137695-63137714 None:intergenic 50.0%
ACCATATCCTCCGGAAGGAA+CGG + chr2_4:63138017-63138036 None:intergenic 50.0%
AATGTTCGTTGTCACGGCAG+TGG - chr2_4:63138075-63138094 Msa0341490:CDS 50.0%
GGAAGATGAAGCCGACATAG+GGG - chr2_4:63138190-63138209 Msa0341490:intron 50.0%
TCATGACAAGTGGGGATAGG+GGG + chr2_4:63138436-63138455 None:intergenic 50.0%
GCTGGACCTAGAGTTCTTAC+TGG + chr2_4:63138514-63138533 None:intergenic 50.0%
GCTCCAATACTTGGAGCTCT+AGG - chr2_4:63138577-63138596 Msa0341490:intron 50.0%
CCACCTAGAGCTCCAAGTAT+TGG + chr2_4:63138583-63138602 None:intergenic 50.0%
!! CCAATACTTGGAGCTCTAGG+TGG - chr2_4:63138580-63138599 Msa0341490:intron 50.0%
AGGCATGATGCCTGTTTGGA+TGG - chr2_4:63139343-63139362 Msa0341490:five_prime_UTR 50.0%
GGCATGATGCCTGTTTGGAT+GGG - chr2_4:63139344-63139363 Msa0341490:five_prime_UTR 50.0%
AGCATGTGGAAGTGGACGGT+GGG + chr2_4:63136911-63136930 None:intergenic 55.0%
!! CCTGGTGTTGCTGGTGTTGA+TGG + chr2_4:63137181-63137200 None:intergenic 55.0%
CCATCAACACCAGCAACACC+AGG - chr2_4:63137178-63137197 Msa0341490:three_prime_UTR 55.0%
AGGGGGTGGCATTAATGAGC+AGG + chr2_4:63137350-63137369 None:intergenic 55.0%
! TTGCAAGAGGCACAGGAGGA+GGG + chr2_4:63137369-63137388 None:intergenic 55.0%
! TTTGCAAGAGGCACAGGAGG+AGG + chr2_4:63137370-63137389 None:intergenic 55.0%
CAAGGCTCGGAGACTCTGAT+TGG - chr2_4:63137618-63137637 Msa0341490:intron 55.0%
ATGAGCACCAGAGATGTGGC+CGG + chr2_4:63137691-63137710 None:intergenic 55.0%
! CTGCATTGAAGCACTTCCCG+TGG - chr2_4:63137739-63137758 Msa0341490:intron 55.0%
ACCGTTCCTTCCGGAGGATA+TGG - chr2_4:63138013-63138032 Msa0341490:CDS 55.0%
GCTTGACCATATCCTCCGGA+AGG + chr2_4:63138022-63138041 None:intergenic 55.0%
ACTCACAGCTCTTGTGTCGG+TGG + chr2_4:63138101-63138120 None:intergenic 55.0%
AAGTGGGGATAGGGGGAAGA+AGG + chr2_4:63138429-63138448 None:intergenic 55.0%
! ATGATGGTGCACTGCGTTGC+AGG - chr2_4:63138464-63138483 Msa0341490:CDS 55.0%
CTGCGTTGCAGGCCAATAAC+AGG - chr2_4:63138475-63138494 Msa0341490:CDS 55.0%
! CCAGTGTTGGGACTCGTTTG+CGG - chr2_4:63139214-63139233 Msa0341490:five_prime_UTR 55.0%
! CAGTGTTGGGACTCGTTTGC+GGG - chr2_4:63139215-63139234 Msa0341490:five_prime_UTR 55.0%
!! GACGGTGGGACAGTTCTGAG+GGG + chr2_4:63136897-63136916 None:intergenic 60.0%
! GGACGGTGGGACAGTTCTGA+GGG + chr2_4:63136898-63136917 None:intergenic 60.0%
TGGACGGTGGGACAGTTCTG+AGG + chr2_4:63136899-63136918 None:intergenic 60.0%
CAGCATGTGGAAGTGGACGG+TGG + chr2_4:63136912-63136931 None:intergenic 60.0%
!! AGAGGTACACCAGGAGTGCC+TGG + chr2_4:63137199-63137218 None:intergenic 60.0%
! TGCAAGAGGCACAGGAGGAG+GGG + chr2_4:63137368-63137387 None:intergenic 60.0%
GGTGGAGTCACCGTTCCTTC+CGG - chr2_4:63138004-63138023 Msa0341490:CDS 60.0%
CCGCAAACGAGTCCCAACAC+TGG + chr2_4:63139217-63139236 None:intergenic 60.0%
!! CCAGGAGTGCCTGGTGTTGC+TGG + chr2_4:63137190-63137209 None:intergenic 65.0%
CCAGCAACACCAGGCACTCC+TGG - chr2_4:63137187-63137206 Msa0341490:three_prime_UTR 65.0%
! GCAAGAGGCACAGGAGGAGG+GGG + chr2_4:63137367-63137386 None:intergenic 65.0%
CAGAGATGTGGCCGGTGGAG+AGG + chr2_4:63137683-63137702 None:intergenic 65.0%
CTCTCCACCGGCCACATCTC+TGG - chr2_4:63137681-63137700 Msa0341490:CDS 65.0%
AGCACCAGAGATGTGGCCGG+TGG + chr2_4:63137688-63137707 None:intergenic 65.0%
GGAGTCACCGTTCCTTCCGG+AGG - chr2_4:63138007-63138026 Msa0341490:CDS 65.0%
!! CTTGGAGCTCTAGGTGGGGC+AGG - chr2_4:63138586-63138605 Msa0341490:intron 65.0%
! AGAGGCACAGGAGGAGGGGG+TGG + chr2_4:63137364-63137383 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 63136545 63139498 63136545 ID=Msa0341490;Name=Msa0341490
chr2_4 mRNA 63136545 63139498 63136545 ID=Msa0341490-mRNA-1;Parent=Msa0341490;Name=Msa0341490-mRNA-1;_AED=0.05;_eAED=0.05;_QI=606|1|1|1|0.8|0.66|6|765|310
chr2_4 exon 63136545 63137243 63136545 ID=Msa0341490-mRNA-1:exon:20141;Parent=Msa0341490-mRNA-1
chr2_4 exon 63137284 63137557 63137284 ID=Msa0341490-mRNA-1:exon:20140;Parent=Msa0341490-mRNA-1
chr2_4 exon 63137665 63137726 63137665 ID=Msa0341490-mRNA-1:exon:20139;Parent=Msa0341490-mRNA-1
chr2_4 exon 63137929 63138123 63137929 ID=Msa0341490-mRNA-1:exon:20138;Parent=Msa0341490-mRNA-1
chr2_4 exon 63138276 63138500 63138276 ID=Msa0341490-mRNA-1:exon:20137;Parent=Msa0341490-mRNA-1
chr2_4 exon 63138650 63139498 63138650 ID=Msa0341490-mRNA-1:exon:20136;Parent=Msa0341490-mRNA-1
chr2_4 five_prime_UTR 63138893 63139498 63138893 ID=Msa0341490-mRNA-1:five_prime_utr;Parent=Msa0341490-mRNA-1
chr2_4 CDS 63138650 63138892 63138650 ID=Msa0341490-mRNA-1:cds;Parent=Msa0341490-mRNA-1
chr2_4 CDS 63138276 63138500 63138276 ID=Msa0341490-mRNA-1:cds;Parent=Msa0341490-mRNA-1
chr2_4 CDS 63137929 63138123 63137929 ID=Msa0341490-mRNA-1:cds;Parent=Msa0341490-mRNA-1
chr2_4 CDS 63137665 63137726 63137665 ID=Msa0341490-mRNA-1:cds;Parent=Msa0341490-mRNA-1
chr2_4 CDS 63137350 63137557 63137350 ID=Msa0341490-mRNA-1:cds;Parent=Msa0341490-mRNA-1
chr2_4 three_prime_UTR 63137284 63137349 63137284 ID=Msa0341490-mRNA-1:three_prime_utr;Parent=Msa0341490-mRNA-1
chr2_4 three_prime_UTR 63136545 63137243 63136545 ID=Msa0341490-mRNA-1:three_prime_utr;Parent=Msa0341490-mRNA-1
Gene Sequence

>Msa0341490

ATGGAACATATTAATAATGAGGAAATTCCATCAACACCAGCAACACCAGGCACTCCTGGTGTACCTCTTTTTGGTGGGTTTAAGTCTGAGAGAAGCGGGAATGGTAATAATAAATATAAGAAGTCCCTACTCAAGAATTGTAAATGCTTCGGTGTCCAAGAATGGAACTTAGAAGATGGAGCTCTACCCACAGTTACCTGCTCATTAATGCCACCCCCTCCTCCTGTGCCTCTTGCAAAAAAGATAGGAGCCGAGTTTATAGGGACATTGATTCTGATGTTTGCTGGGACAGCCACTGCTATTGTGAACCAAAAGACACAAGGCTCGGAGACTCTGATTGGGTGTGCTACCTCTACTGGTCTTGCGGTTATGATCATCATCCTCTCCACCGGCCACATCTCTGGTGCTCATCTCAACCCTGCAGTCACCATTTCTTTTGCTGCATTGAAGCACTTCCCGTGGAAGCATGTGCCTATGTATATTGGTGCACAAGTTTTGGCATCAATATGTGCTGCATTTGCTCTCAAAGGGGTTTTTCACCCTTTTATGAGTGGTGGAGTCACCGTTCCTTCCGGAGGATATGGTCAAGCTTTTGCTTTAGAATTCATTATCAGCTTTAATCTAATGTTCGTTGTCACGGCAGTGGCCACCGACACAAGAGCTGTAGGAGAACTAGCAGGAATTGCAGTGGGAGCAACTGTGATGCTCAATATACTAATAGCAGGGCCAATAACAGGAGCGTCAATGAACCCAGTAAGAACTCTAGGTCCAGCTATAGCTGCAAATAACTACAAAGCCATATGGGTCTATCTCTTAGCTCCAATACTTGGAGCTCTAGGTGGGGCAGGAACTTATACTGCAGTCAAGCTTCCCGAAGAAGATGATAATGCAAAGACTAATGCTTCTTCAAATCACCCCAGCTTTAGAAGATGA
Protein sequence

>Msa0341490

MEHINNEEIPSTPATPGTPGVPLFGGFKSERSGNGNNKYKKSLLKNCKCFGVQEWNLEDGALPTVTCSLMPPPPPVPLAKKIGAEFIGTLILMFAGTATAIVNQKTQGSETLIGCATSTGLAVMIIILSTGHISGAHLNPAVTISFAALKHFPWKHVPMYIGAQVLASICAAFALKGVFHPFMSGGVTVPSGGYGQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAGPITGASMNPVRTLGPAIAANNYKAIWVYLLAPILGALGGAGTYTAVKLPEEDDNAKTNASSNHPSFRR*