Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0044090 | Msa1124160 | 0.800322 | 1.522109e-48 | -8.615850e-47 |
| Msa0104800 | Msa1124160 | 0.801828 | 7.488600e-49 | -8.615850e-47 |
| Msa0106620 | Msa1124160 | 0.805883 | 1.076777e-49 | -8.615850e-47 |
| Msa0106710 | Msa1124160 | 0.818025 | 2.430561e-52 | -8.615850e-47 |
| Msa0622120 | Msa1124160 | 0.810247 | 1.267299e-50 | -8.615850e-47 |
| Msa0624060 | Msa1124160 | 0.816387 | 5.676211e-52 | -8.615850e-47 |
| Msa0624470 | Msa1124160 | 0.806605 | 7.586309e-50 | -8.615850e-47 |
| Msa0676130 | Msa1124160 | 0.809830 | 1.558483e-50 | -8.615850e-47 |
| Msa0676180 | Msa1124160 | 0.813606 | 2.350958e-51 | -8.615850e-47 |
| Msa0721280 | Msa1124160 | 0.820114 | 8.137541e-53 | -8.615850e-47 |
| Msa0756380 | Msa1124160 | 0.815907 | 7.266645e-52 | -8.615850e-47 |
| Msa0763890 | Msa1124160 | 0.812963 | 3.254114e-51 | -8.615850e-47 |
| Msa0763920 | Msa1124160 | 0.802820 | 4.679589e-49 | -8.615850e-47 |
| Msa0178570 | Msa1124160 | 0.805936 | 1.049490e-49 | -8.615850e-47 |
| Msa0183420 | Msa1124160 | 0.802868 | 4.573398e-49 | -8.615850e-47 |
| Msa0183760 | Msa1124160 | 0.805296 | 1.429828e-49 | -8.615850e-47 |
| Msa0206010 | Msa1124160 | 0.808798 | 2.594721e-50 | -8.615850e-47 |
| Msa0389910 | Msa1124160 | 0.809252 | 2.074020e-50 | -8.615850e-47 |
| Msa0455760 | Msa1124160 | 0.808187 | 3.503005e-50 | -8.615850e-47 |
| Msa0986510 | Msa1124160 | 0.806063 | 9.867601e-50 | -8.615850e-47 |
| Msa0249310 | Msa1124160 | 0.800541 | 1.373226e-48 | -8.615850e-47 |
| Msa0326110 | Msa1124160 | 0.805961 | 1.036618e-49 | -8.615850e-47 |
| Msa0347230 | Msa1124160 | 0.858006 | 1.107716e-62 | -8.615850e-47 |
| Msa0347950 | Msa1124160 | 0.802353 | 5.842009e-49 | -8.615850e-47 |
| Msa0347990 | Msa1124160 | 0.801011 | 1.100836e-48 | -8.615850e-47 |
| Msa0348530 | Msa1124160 | 0.807323 | 5.346823e-50 | -8.615850e-47 |
| Msa1124160 | Msa1304940 | 0.800876 | 1.173095e-48 | -8.615850e-47 |
| Msa1124160 | Msa1357890 | 0.817487 | 3.214336e-52 | -8.615850e-47 |
| Msa1124160 | Msa1372240 | 0.801645 | 8.167013e-49 | -8.615850e-47 |
| Msa1124160 | Msa1378410 | 0.813782 | 2.150636e-51 | -8.615850e-47 |
| Msa1124160 | Msa1398090 | 0.809346 | 1.980234e-50 | -8.615850e-47 |
| Msa1124160 | Msa1400120 | 0.815905 | 7.276261e-52 | -8.615850e-47 |
| Msa0526020 | Msa1124160 | 0.809513 | 1.823439e-50 | -8.615850e-47 |
| Msa0581400 | Msa1124160 | 0.804439 | 2.159103e-49 | -8.615850e-47 |
| Msa0866400 | Msa1124160 | 0.803149 | 4.001028e-49 | -8.615850e-47 |
| Msa0866410 | Msa1124160 | 0.820136 | 8.045428e-53 | -8.615850e-47 |
| Msa0934140 | Msa1124160 | 0.802306 | 5.971919e-49 | -8.615850e-47 |
| Msa0936390 | Msa1124160 | 0.813069 | 3.084260e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATAGATGTTGATTGAAAT+TGG | 0.232139 | 7_3:+75280102 | None:intergenic |
| TGTGAGTTGTTACGTGAAAT+AGG | 0.334697 | 7_3:+75280188 | None:intergenic |
| AAGGAGAGGAGCACGGAAAC+TGG | 0.341508 | 7_3:-75279975 | Msa1124160:five_prime_UTR |
| GTTTCCGTGCTCCTCTCCTT+TGG | 0.345017 | 7_3:+75279978 | None:intergenic |
| GTGTGTTGTTATGCTGGATT+CGG | 0.357976 | 7_3:+75280140 | None:intergenic |
| AGCTGAACCGGTTCAGGAAC+CGG | 0.361281 | 7_3:+75279666 | None:intergenic |
| AAGAAAAGCTGAACCGGTTC+AGG | 0.362146 | 7_3:+75279660 | None:intergenic |
| TACCTCTTCCTTCTCAGCTT+CGG | 0.371599 | 7_3:+75279893 | None:intergenic |
| ATAGAGAGAGAGAAGAGAAT+TGG | 0.376035 | 7_3:-75279794 | Msa1124160:intron |
| TTGTATTTGCTGAACCGGTT+CGG | 0.381246 | 7_3:-75279699 | Msa1124160:CDS |
| AACCGACCCGGTTCCTGAAC+CGG | 0.403375 | 7_3:-75279673 | Msa1124160:CDS |
| TGTGTTGTTATGCTGGATTC+GGG | 0.411143 | 7_3:+75280141 | None:intergenic |
| GCTTATGTGTGTTGTTATGC+TGG | 0.414463 | 7_3:+75280134 | None:intergenic |
| AAGGAGTAAAACTGTGGGCT+TGG | 0.438906 | 7_3:+75279633 | None:intergenic |
| TCTCCTTTGGATCGAACAAA+AGG | 0.474957 | 7_3:+75279991 | None:intergenic |
| AGAGAGAAGAGAATTGGCGT+TGG | 0.477876 | 7_3:-75279788 | Msa1124160:five_prime_UTR |
| CGCAAGCTATTTCGAACTGA+TGG | 0.478346 | 7_3:-75280048 | Msa1124160:five_prime_UTR |
| GGAGTAAAGAAAAGCTGAAC+CGG | 0.489718 | 7_3:+75279654 | None:intergenic |
| CCGGGTCGGTTCGACCGAAC+CGG | 0.496072 | 7_3:+75279685 | None:intergenic |
| GAGCAGATCCGAAGCTGAGA+AGG | 0.503469 | 7_3:-75279901 | Msa1124160:five_prime_UTR |
| GCTGAACCGGTTCAGGAACC+GGG | 0.538766 | 7_3:+75279667 | None:intergenic |
| TGGGAACAGAGGAATGAAAC+GGG | 0.563195 | 7_3:-75279731 | Msa1124160:five_prime_UTR |
| GTGGGAACAGAGGAATGAAA+CGG | 0.565654 | 7_3:-75279732 | Msa1124160:five_prime_UTR |
| ATGTTGATTGAAATTGGAAG+TGG | 0.577999 | 7_3:+75280108 | None:intergenic |
| ATCCGAAGCTGAGAAGGAAG+AGG | 0.578030 | 7_3:-75279895 | Msa1124160:intron |
| CCGGTTCGGTCGAACCGACC+CGG | 0.579998 | 7_3:-75279685 | Msa1124160:CDS |
| GAGGAGCACGGAAACTGGAT+CGG | 0.581402 | 7_3:-75279970 | Msa1124160:five_prime_UTR |
| TGTTGGTGGCGTGGGAACAG+AGG | 0.609038 | 7_3:-75279742 | Msa1124160:five_prime_UTR |
| CCTTTGGATCGAACAAAAGG+AGG | 0.610982 | 7_3:+75279994 | None:intergenic |
| CGATCCAAAGGAGAGGAGCA+CGG | 0.618840 | 7_3:-75279982 | Msa1124160:five_prime_UTR |
| AACCGGTTCAGGAACCGGGT+CGG | 0.646620 | 7_3:+75279671 | None:intergenic |
| TGGGCCAAGGAGTAAAACTG+TGG | 0.647218 | 7_3:+75279627 | None:intergenic |
| GGGCCAAGGAGTAAAACTGT+GGG | 0.674862 | 7_3:+75279628 | None:intergenic |
| GGAACGAATCGATCTCAGCA+AGG | 0.681192 | 7_3:-75280027 | Msa1124160:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAACCAAAGAAAGAAAAA+AGG | + | chr7_3:75279670-75279689 | None:intergenic | 20.0% |
| !!! | ATATAGATGTTGATTGAAAT+TGG | + | chr7_3:75279732-75279751 | None:intergenic | 20.0% |
| !!! | TATATTTTCGAAAATCAACA+GGG | - | chr7_3:75279747-75279766 | Msa1124160:five_prime_UTR | 20.0% |
| !!! | TTGAAGAAATTGAATTTTGT+TGG | - | chr7_3:75280072-75280091 | Msa1124160:five_prime_UTR | 20.0% |
| !! | CTATATTTTCGAAAATCAAC+AGG | - | chr7_3:75279746-75279765 | Msa1124160:five_prime_UTR | 25.0% |
| ! | ACACAAACATTAAGATGAAT+GGG | + | chr7_3:75279984-75280003 | None:intergenic | 25.0% |
| !! | AAGAAATTGAATTTTGTTGG+TGG | - | chr7_3:75280075-75280094 | Msa1124160:five_prime_UTR | 25.0% |
| ! | ATGTTGATTGAAATTGGAAG+TGG | + | chr7_3:75279726-75279745 | None:intergenic | 30.0% |
| TAAGATGAATGGGGAAAAAA+AGG | + | chr7_3:75279974-75279993 | None:intergenic | 30.0% | |
| CACAAACATTAAGATGAATG+GGG | + | chr7_3:75279983-75280002 | None:intergenic | 30.0% | |
| CACACAAACATTAAGATGAA+TGG | + | chr7_3:75279985-75280004 | None:intergenic | 30.0% | |
| CATTCATCTTAATGTTTGTG+TGG | - | chr7_3:75279983-75280002 | Msa1124160:five_prime_UTR | 30.0% | |
| !! | GTGGTATTCTTTTTGAAAGT+TGG | - | chr7_3:75280002-75280021 | Msa1124160:five_prime_UTR | 30.0% |
| TGTGAGTTGTTACGTGAAAT+AGG | + | chr7_3:75279646-75279665 | None:intergenic | 35.0% | |
| !!! | CACAGCCTTTTTTCTTTCTT+TGG | - | chr7_3:75279662-75279681 | Msa1124160:CDS | 35.0% |
| ATAGAGAGAGAGAAGAGAAT+TGG | - | chr7_3:75280037-75280056 | Msa1124160:five_prime_UTR | 35.0% | |
| !! | TGCTTTTGTATTTGCTGAAC+CGG | - | chr7_3:75280127-75280146 | Msa1124160:five_prime_UTR | 35.0% |
| ! | TGTGTTGTTATGCTGGATTC+GGG | + | chr7_3:75279693-75279712 | None:intergenic | 40.0% |
| ! | GTGTGTTGTTATGCTGGATT+CGG | + | chr7_3:75279694-75279713 | None:intergenic | 40.0% |
| ! | GCTTATGTGTGTTGTTATGC+TGG | + | chr7_3:75279700-75279719 | None:intergenic | 40.0% |
| TCTCCTTTGGATCGAACAAA+AGG | + | chr7_3:75279843-75279862 | None:intergenic | 40.0% | |
| ! | TTTTGTTCGATCCAAAGGAG+AGG | - | chr7_3:75279842-75279861 | Msa1124160:intron | 40.0% |
| !!! | ATTGAATTTTGTTGGTGGCG+TGG | - | chr7_3:75280080-75280099 | Msa1124160:five_prime_UTR | 40.0% |
| !!! | TTGAATTTTGTTGGTGGCGT+GGG | - | chr7_3:75280081-75280100 | Msa1124160:five_prime_UTR | 40.0% |
| ! | TTGTATTTGCTGAACCGGTT+CGG | - | chr7_3:75280132-75280151 | Msa1124160:five_prime_UTR | 40.0% |
| GGAGTAAAGAAAAGCTGAAC+CGG | + | chr7_3:75280180-75280199 | None:intergenic | 40.0% | |
| ! | CGCAAGCTATTTCGAACTGA+TGG | - | chr7_3:75279783-75279802 | Msa1124160:five_prime_UTR | 45.0% |
| CCTTTGGATCGAACAAAAGG+AGG | + | chr7_3:75279840-75279859 | None:intergenic | 45.0% | |
| ! | CCTCCTTTTGTTCGATCCAA+AGG | - | chr7_3:75279837-75279856 | Msa1124160:intron | 45.0% |
| TACCTCTTCCTTCTCAGCTT+CGG | + | chr7_3:75279941-75279960 | None:intergenic | 45.0% | |
| AGAGAGAAGAGAATTGGCGT+TGG | - | chr7_3:75280043-75280062 | Msa1124160:five_prime_UTR | 45.0% | |
| GTGGGAACAGAGGAATGAAA+CGG | - | chr7_3:75280099-75280118 | Msa1124160:five_prime_UTR | 45.0% | |
| TGGGAACAGAGGAATGAAAC+GGG | - | chr7_3:75280100-75280119 | Msa1124160:five_prime_UTR | 45.0% | |
| ! | AAGAAAAGCTGAACCGGTTC+AGG | + | chr7_3:75280174-75280193 | None:intergenic | 45.0% |
| GGAACGAATCGATCTCAGCA+AGG | - | chr7_3:75279804-75279823 | Msa1124160:intron | 50.0% | |
| ATCCGAAGCTGAGAAGGAAG+AGG | - | chr7_3:75279936-75279955 | Msa1124160:five_prime_UTR | 50.0% | |
| CGATCCAAAGGAGAGGAGCA+CGG | - | chr7_3:75279849-75279868 | Msa1124160:intron | 55.0% | |
| GTTTCCGTGCTCCTCTCCTT+TGG | + | chr7_3:75279856-75279875 | None:intergenic | 55.0% | |
| ! | AAGGAGAGGAGCACGGAAAC+TGG | - | chr7_3:75279856-75279875 | Msa1124160:intron | 55.0% |
| ! | GAGGAGCACGGAAACTGGAT+CGG | - | chr7_3:75279861-75279880 | Msa1124160:intron | 55.0% |
| GAGCAGATCCGAAGCTGAGA+AGG | - | chr7_3:75279930-75279949 | Msa1124160:five_prime_UTR | 55.0% | |
| ! | AGCTGAACCGGTTCAGGAAC+CGG | + | chr7_3:75280168-75280187 | None:intergenic | 55.0% |
| !! | TGTTGGTGGCGTGGGAACAG+AGG | - | chr7_3:75280089-75280108 | Msa1124160:five_prime_UTR | 60.0% |
| AACCGACCCGGTTCCTGAAC+CGG | - | chr7_3:75280158-75280177 | Msa1124160:five_prime_UTR | 60.0% | |
| ! | AACCGGTTCAGGAACCGGGT+CGG | + | chr7_3:75280163-75280182 | None:intergenic | 60.0% |
| ! | GCTGAACCGGTTCAGGAACC+GGG | + | chr7_3:75280167-75280186 | None:intergenic | 60.0% |
| !! | CCGGGTCGGTTCGACCGAAC+CGG | + | chr7_3:75280149-75280168 | None:intergenic | 70.0% |
| !! | CCGGTTCGGTCGAACCGACC+CGG | - | chr7_3:75280146-75280165 | Msa1124160:five_prime_UTR | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 75279641 | 75280212 | 75279641 | ID=Msa1124160;Name=Msa1124160 |
| chr7_3 | mRNA | 75279641 | 75280212 | 75279641 | ID=Msa1124160-mRNA-1;Parent=Msa1124160;Name=Msa1124160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=402|1|0.5|1|1|1|2|0|29 |
| chr7_3 | exon | 75279641 | 75279812 | 75279641 | ID=Msa1124160-mRNA-1:exon:20210;Parent=Msa1124160-mRNA-1 |
| chr7_3 | exon | 75279896 | 75280212 | 75279896 | ID=Msa1124160-mRNA-1:exon:20209;Parent=Msa1124160-mRNA-1 |
| chr7_3 | five_prime_UTR | 75279896 | 75280212 | 75279896 | ID=Msa1124160-mRNA-1:five_prime_utr;Parent=Msa1124160-mRNA-1 |
| chr7_3 | five_prime_UTR | 75279728 | 75279812 | 75279728 | ID=Msa1124160-mRNA-1:five_prime_utr;Parent=Msa1124160-mRNA-1 |
| chr7_3 | CDS | 75279641 | 75279727 | 75279641 | ID=Msa1124160-mRNA-1:cds;Parent=Msa1124160-mRNA-1 |
| Gene Sequence |
| Protein sequence |
