AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0419960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0419960 MtrunA17_Chr3g0118651 94.592 906 44 2 1 904 1 903 0.0 1753
Msa0419960 MtrunA17_Chr3g0118641 76.652 908 202 6 1 904 1 902 0.0 1453
Msa0419960 MtrunA17_Chr3g0118621 74.646 848 198 7 69 904 23 865 0.0 1274
Msa0419960 MtrunA17_Chr4g0033171 64.598 709 242 5 198 904 24 725 0.0 990
Msa0419960 MtrunA17_Chr8g0344201 47.636 846 415 12 76 903 91 926 0.0 813
Msa0419960 MtrunA17_Chr8g0377101 47.170 848 418 14 76 904 80 916 0.0 801
Msa0419960 MtrunA17_Chr1g0191921 43.105 863 462 13 62 904 5 858 0.0 696
Msa0419960 MtrunA17_Chr1g0205611 45.616 787 397 13 136 904 83 856 0.0 687
Msa0419960 MtrunA17_Chr4g0047281 42.081 884 454 18 42 903 47 894 0.0 678
Msa0419960 MtrunA17_Chr8g0342881 43.504 839 433 17 86 904 39 856 0.0 673
Msa0419960 MtrunA17_Chr8g0342971 43.073 859 435 18 80 904 32 870 0.0 672
Msa0419960 MtrunA17_Chr8g0343021 43.198 838 437 15 88 904 45 864 0.0 671
Msa0419960 MtrunA17_Chr8g0343031 42.823 829 438 15 92 904 57 865 0.0 671
Msa0419960 MtrunA17_Chr2g0329911 43.341 826 436 15 94 904 53 861 0.0 669
Msa0419960 MtrunA17_Chr8g0342921 42.972 868 443 17 60 904 31 869 0.0 665
Msa0419960 MtrunA17_Chr5g0406871 41.628 872 464 17 56 904 18 867 0.0 659
Msa0419960 MtrunA17_Chr2g0329901 42.909 832 441 15 89 904 50 863 0.0 659
Msa0419960 MtrunA17_Chr7g0272791 43.185 829 437 16 97 904 52 867 0.0 657
Msa0419960 MtrunA17_Chr8g0343011 44.228 771 409 11 139 904 111 865 0.0 657
Msa0419960 MtrunA17_Chr8g0342891 45.174 777 398 13 139 904 102 861 0.0 651
Msa0419960 MtrunA17_Chr8g0342931 45.241 767 392 14 149 904 1 750 0.0 649
Msa0419960 MtrunA17_Chr8g0344211 43.606 782 413 12 134 904 104 868 0.0 646
Msa0419960 MtrunA17_Chr8g0342901 41.935 837 437 16 80 904 29 828 0.0 640
Msa0419960 MtrunA17_Chr8g0342951 44.114 773 404 12 143 904 113 868 0.0 629
Msa0419960 MtrunA17_Chr3g0120071 40.980 837 457 16 88 904 39 858 0.0 620
Msa0419960 MtrunA17_Chr8g0342981 42.893 788 399 19 134 904 70 823 0.0 595
Msa0419960 MtrunA17_Chr8g0342991 42.670 764 379 11 146 904 98 807 0.0 570
Msa0419960 MtrunA17_Chr8g0343001 42.016 764 384 11 146 904 98 807 0.0 556
Msa0419960 MtrunA17_Chr8g0338451 38.758 805 446 19 110 887 66 850 0.0 551
Msa0419960 MtrunA17_Chr8g0344241 45.025 402 207 5 510 904 67 461 6.63e-113 354
Msa0419960 MtrunA17_Chr6g0471951 37.251 502 262 10 366 859 18 474 1.14e-105 336
Msa0419960 MtrunA17_Chr7g0218231 97.479 119 3 0 554 672 14 132 1.00e-76 246
Msa0419960 MtrunA17_Chr6g0468081 97.753 89 2 0 584 672 1 89 3.14e-54 182
Msa0419960 MtrunA17_Chr4g0033181 47.531 162 75 3 61 213 60 220 6.76e-40 147
Msa0419960 MtrunA17_Chr4g0047271 46.154 91 48 1 814 903 1 91 9.37e-18 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0419960 AT3G45140.1 52.839 863 373 10 60 904 50 896 0.0 937
Msa0419960 AT3G45140.2 56.732 765 315 7 145 904 1 754 0.0 914
Msa0419960 AT1G72520.1 47.585 849 411 17 76 904 92 926 0.0 799
Msa0419960 AT1G17420.1 45.909 880 444 13 45 904 52 919 0.0 796
Msa0419960 AT1G67560.1 46.806 814 410 13 103 904 115 917 0.0 756
Msa0419960 AT1G55020.1 45.300 883 436 17 41 904 5 859 0.0 716
Msa0419960 AT3G22400.1 43.697 833 428 15 101 904 66 886 0.0 681

Find 191 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAGCAATGTTAAATTAT+TGG 0.149707 3_2:+68164180 None:intergenic
ATTCTGGTGGACCATAAATT+TGG 0.173285 3_2:+68163735 None:intergenic
CTAGCAATTGTAGGTCTATT+AGG 0.189376 3_2:+68162449 None:intergenic
AAATTGGATGCCCAAATTTA+TGG 0.209486 3_2:-68163746 Msa0419960:CDS
CAATTATTGCCTTGACTAAT+TGG 0.209625 3_2:+68163881 None:intergenic
TTTGAGTTACCAGCAGATTT+TGG 0.210007 3_2:-68165291 Msa0419960:CDS
TTGACTAATTGGATGCTATT+TGG 0.210653 3_2:+68163892 None:intergenic
TGACTAATTGGATGCTATTT+GGG 0.253957 3_2:+68163893 None:intergenic
CTTCTGATTCTACCAATCTT+TGG 0.255433 3_2:-68163360 Msa0419960:CDS
GAGCTCACTAGACCGATTAT+TGG 0.258181 3_2:-68163418 Msa0419960:CDS
TCAAATGCTACCTTTATATC+TGG 0.259677 3_2:+68162230 None:intergenic
GTAGCATTTGAAAGGTTTAA+TGG 0.262850 3_2:-68162218 Msa0419960:CDS
TATAAGATATCTTGGGATAT+TGG 0.270541 3_2:-68166006 Msa0419960:five_prime_UTR
GTTGCTACAACAAAAGTAAT+TGG 0.272082 3_2:-68165878 Msa0419960:CDS
AAATGTTGCATTAATTGTTA+TGG 0.276226 3_2:-68162026 Msa0419960:three_prime_UTR
AACTTTGCACAATATGCTTA+TGG 0.278802 3_2:-68162482 Msa0419960:CDS
CAAACCTGAGCAAACACTTT+TGG 0.281831 3_2:-68162372 Msa0419960:CDS
CTAAATAACATAGTCCTTGA+TGG 0.285243 3_2:-68165219 Msa0419960:CDS
ATGGCCTCTGAGAAGTAAAT+TGG 0.305851 3_2:-68163762 Msa0419960:CDS
GTGAAGAAAACTCTCTTTGT+AGG 0.307177 3_2:+68165135 None:intergenic
TTTAGGCCATGTTGTGCATT+AGG 0.307604 3_2:+68162779 None:intergenic
CAAATGATGGTCTTCTCATA+TGG 0.312417 3_2:-68162730 Msa0419960:CDS
GATTCAATGATGCTTGGATT+TGG 0.313188 3_2:+68162662 None:intergenic
TAGCAATTGTAGGTCTATTA+GGG 0.314837 3_2:+68162450 None:intergenic
TAAATTGCATTTATCTCCTC+TGG 0.315738 3_2:+68166050 None:intergenic
GTGACTCCAGGTCCTGAAAA+TGG 0.317105 3_2:+68162095 None:intergenic
ATTCTACCAATCTTTGGCTT+TGG 0.318489 3_2:-68163354 Msa0419960:CDS
CATCTTCACCTTCCTATATA+AGG 0.323869 3_2:-68165917 Msa0419960:CDS
TATCAAAGCGCAAAAGGTTT+TGG 0.327729 3_2:+68164119 None:intergenic
TGGAAATGTGGGCGCTGTTT+TGG 0.329432 3_2:-68165271 Msa0419960:CDS
TTCAAATGAAGGAACAAATT+CGG 0.330717 3_2:-68165385 Msa0419960:intron
CCTTTGTGATTGCAACAAAT+AGG 0.332818 3_2:-68163157 Msa0419960:CDS
TGCATTAATTGTTATGGAAT+AGG 0.336101 3_2:-68162020 Msa0419960:three_prime_UTR
TAGTACTAGTACAAGTAAAA+AGG 0.340965 3_2:-68165829 Msa0419960:CDS
CTACCAATCTTTGGCTTTGG+AGG 0.343171 3_2:-68163351 Msa0419960:CDS
AAACTCAAAAGACCTGTTCT+TGG 0.345034 3_2:-68164796 Msa0419960:CDS
GCTTTGGCAATTTGATTTGC+AGG 0.346703 3_2:-68162964 Msa0419960:CDS
TCAACAAAAGCGTCATAGTT+TGG 0.349961 3_2:+68165979 None:intergenic
GTCATCGAGTCGCAGATAAT+TGG 0.351690 3_2:-68163695 Msa0419960:CDS
TACTAAATAGATATGCTATA+TGG 0.354697 3_2:+68162059 None:intergenic
TTCTCCCATTCTTCCTTTGT+AGG 0.357198 3_2:+68162404 None:intergenic
CTCATCAAGAATATTGTTAC+TGG 0.359156 3_2:-68165708 Msa0419960:CDS
TGGCTTCAATGTGCCTTGTT+TGG 0.360768 3_2:+68163450 None:intergenic
TTGCAGGAGACAAATTCTTT+TGG 0.363534 3_2:-68163960 Msa0419960:intron
TAGCATTTGAAAGGTTTAAT+GGG 0.363606 3_2:-68162217 Msa0419960:CDS
TAACCAACTATAAGATATCT+TGG 0.364041 3_2:-68166014 Msa0419960:five_prime_UTR
TCCCTCACTTTGCTTACATA+TGG 0.365328 3_2:+68163520 None:intergenic
AATATTGTTACTGGTATTGT+TGG 0.378392 3_2:-68165699 Msa0419960:CDS
AGTCTTCTATTGCTAGCTTT+AGG 0.378934 3_2:+68162762 None:intergenic
ATTCTCACAGACATAAATCT+TGG 0.383754 3_2:-68164271 Msa0419960:CDS
AAACAATTTCTTCTCTTGAA+TGG 0.386249 3_2:+68163570 None:intergenic
TTTGTAGGAGTATCATGCTT+GGG 0.390346 3_2:+68165150 None:intergenic
TCTCTCGTGATAGCTGATTC+TGG 0.393236 3_2:+68163719 None:intergenic
GAGATTCTTATTAGTTCATA+TGG 0.393969 3_2:-68163053 Msa0419960:CDS
CCTTGGATACGGATATTGTT+TGG 0.398283 3_2:+68164768 None:intergenic
CTGTGGCATATGATAAGCTT+TGG 0.401553 3_2:-68162980 Msa0419960:CDS
ATGTTGTTTCTAGCAATTGT+AGG 0.408062 3_2:+68162440 None:intergenic
AATGGCTTCATTAGCTCATA+AGG 0.411469 3_2:+68162113 None:intergenic
TCCATCTTAGTTGTCGATCA+TGG 0.412724 3_2:-68165179 Msa0419960:CDS
TTGAGTTTAATGTTGGTCTC+AGG 0.412871 3_2:+68164811 None:intergenic
TTTGCTAGGGAGACTTTGGC+AGG 0.413977 3_2:-68163922 Msa0419960:CDS
GGATGAGAATCCAGATATAA+AGG 0.414714 3_2:-68162240 Msa0419960:CDS
TATGATGTCTACAATGATCT+TGG 0.416200 3_2:-68164838 Msa0419960:CDS
TTTCAATTACCATCATCTCT+TGG 0.418695 3_2:-68164205 Msa0419960:CDS
TGTGGTTTGTCGCCAATAAT+CGG 0.420168 3_2:+68163406 None:intergenic
TTCTGGTGGACCATAAATTT+GGG 0.424260 3_2:+68163736 None:intergenic
TGAGGCGTTTGCTGAGATAA+AGG 0.424456 3_2:-68164359 Msa0419960:CDS
TGATCAGATTCAATGATGCT+TGG 0.424472 3_2:+68162656 None:intergenic
ACCAGTGGCTTATAAGTTCA+TGG 0.425133 3_2:+68163290 None:intergenic
TCTCCCATTCTTCCTTTGTA+GGG 0.428559 3_2:+68162405 None:intergenic
ATCCCAAGATATCTTATAGT+TGG 0.429910 3_2:+68166011 None:intergenic
CTAAGCTTTCTTAATCCACT+TGG 0.430925 3_2:+68164931 None:intergenic
ATGAAGAATACTACACTGTA+TGG 0.431751 3_2:-68163496 Msa0419960:CDS
CACTCCAAAAGTGTTTGCTC+AGG 0.438001 3_2:+68162368 None:intergenic
TTCACCAGATGAAGAGTACT+TGG 0.441500 3_2:-68162285 Msa0419960:CDS
CATGTTCTTACTCATGATAC+TGG 0.449029 3_2:-68163316 Msa0419960:CDS
AGAAGACCATCATTTGCAAA+AGG 0.449584 3_2:+68162737 None:intergenic
GTATGATAATAAGTTCAAAA+TGG 0.450817 3_2:+68162310 None:intergenic
CTAGTGAGCTCAATGACTAT+TGG 0.450930 3_2:+68163430 None:intergenic
CTTTGTAGGAGTATCATGCT+TGG 0.452193 3_2:+68165149 None:intergenic
GGTCGAAAGCATTCTAATAC+AGG 0.461714 3_2:-68164733 Msa0419960:intron
TACCGGTGGAGAAGATCATT+TGG 0.465498 3_2:+68162936 None:intergenic
AAGGTTTAATGGGAGATTGA+AGG 0.467037 3_2:-68162207 Msa0419960:CDS
GGAATTTGCTAGGGAGACTT+TGG 0.467432 3_2:-68163926 Msa0419960:CDS
TGTTTGGTGCCACCAAGAAC+AGG 0.467750 3_2:+68164784 None:intergenic
ACTCCCTTTCCAGTGACTCC+AGG 0.469105 3_2:+68162083 None:intergenic
TGTCCCAAGTACTCTTCATC+TGG 0.470767 3_2:+68162281 None:intergenic
ATGGGATGCAATTAAACAAT+GGG 0.473897 3_2:-68162711 Msa0419960:CDS
AAGACCCTACAAAGGAAGAA+TGG 0.474435 3_2:-68162409 Msa0419960:CDS
CTAATAGAACTAAGAGGGAA+TGG 0.477908 3_2:-68164898 Msa0419960:CDS
GATGAGCTAATAGAACTAAG+AGG 0.478619 3_2:-68164904 Msa0419960:CDS
CTAAGATGGACAGGTCCATC+AGG 0.482847 3_2:+68165192 None:intergenic
TCCTTTGTAGGGTCTTCTGT+AGG 0.484655 3_2:+68162416 None:intergenic
GACTTTAATCAACCTGGGAA+TGG 0.485837 3_2:+68164153 None:intergenic
CTATTTGTTGCAATCACAAA+GGG 0.485842 3_2:+68163158 None:intergenic
AGACCCTACAAAGGAAGAAT+GGG 0.490464 3_2:-68162408 Msa0419960:CDS
ATAGACGAACTTTACAAAGA+AGG 0.490922 3_2:-68164229 Msa0419960:CDS
CATCATCACCACTATAGCTT+GGG 0.495297 3_2:-68162531 Msa0419960:CDS
TAATTGGATGCTATTTGGGT+TGG 0.495555 3_2:+68163897 None:intergenic
GGACCTGGAGTCACTGGAAA+GGG 0.496747 3_2:-68162086 Msa0419960:CDS
CAGGGGTATCAAAGCGCAAA+AGG 0.499404 3_2:+68164113 None:intergenic
TATCCAAGGCGTTGTCGTAC+CGG 0.500621 3_2:-68164754 Msa0419960:CDS
TGGGCAGAAGTTCGAACAAA+AGG 0.502176 3_2:-68162617 Msa0419960:CDS
AGAGAATGAGGTGCAATATG+AGG 0.502465 3_2:-68165319 Msa0419960:CDS
CGACCGGTACGACAACGCCT+TGG 0.509007 3_2:+68164751 None:intergenic
GACAACTCATTGAACCATCA+TGG 0.509837 3_2:-68162262 Msa0419960:CDS
ACATCATCACCACTATAGCT+TGG 0.510325 3_2:-68162532 Msa0419960:CDS
AACACAACCACGGCGTCCTC+AGG 0.513052 3_2:-68164325 Msa0419960:CDS
ATCATACGAAAAGAGAGATT+CGG 0.513208 3_2:-68164401 Msa0419960:CDS
AAGCCTCCAAAGCCAAAGAT+TGG 0.513343 3_2:+68163348 None:intergenic
AACCAACTATAAGATATCTT+GGG 0.519149 3_2:-68166013 Msa0419960:five_prime_UTR
AGTACTCTTCATCTGGTGAA+TGG 0.520631 3_2:+68162288 None:intergenic
CCCACATTTCCAAAATCTGC+TGG 0.525769 3_2:+68165282 None:intergenic
TTTGTTGCCATCACAATGCA+AGG 0.526504 3_2:+68165798 None:intergenic
TCCATGATCGACAACTAAGA+TGG 0.527836 3_2:+68165178 None:intergenic
GTGAATATTTGTTTCCATTG+TGG 0.529219 3_2:+68163388 None:intergenic
CTCAAAAGACCTGTTCTTGG+TGG 0.530119 3_2:-68164793 Msa0419960:CDS
GAACTAAGAGGGAATGGAGA+AGG 0.533603 3_2:-68164892 Msa0419960:CDS
TTCGCAAATTCAAGCAACCT+TGG 0.534313 3_2:-68162339 Msa0419960:CDS
GTACGACAACGCCTTGGATA+CGG 0.536846 3_2:+68164757 None:intergenic
GGGAGATTGAAGGAAATTGA+AGG 0.543222 3_2:-68162197 Msa0419960:CDS
AGATGCTACCCAAGCTATAG+TGG 0.544008 3_2:+68162523 None:intergenic
CATATGGTAAATATAAATCA+TGG 0.544415 3_2:+68163536 None:intergenic
ATATAAAGGTAGCATTTGAA+AGG 0.544570 3_2:-68162226 Msa0419960:CDS
GGATGCAGTAACTTGTAGAT+TGG 0.544887 3_2:+68163116 None:intergenic
GAAGACCATCATTTGCAAAA+GGG 0.545653 3_2:+68162738 None:intergenic
TTGTTCTTCCTCACCAAACA+AGG 0.547255 3_2:-68163463 Msa0419960:CDS
AAATTGCATTTATCTCCTCT+GGG 0.549570 3_2:+68166051 None:intergenic
AATCCACTTGGTGTTTGTGA+TGG 0.549751 3_2:+68164943 None:intergenic
ATTAGCGACTTTAATCAACC+TGG 0.555673 3_2:+68164147 None:intergenic
TATCTGATGAGGAATTTGCT+AGG 0.559728 3_2:-68163936 Msa0419960:CDS
TGCTAGGGAGACTTTGGCAG+GGG 0.560834 3_2:-68163920 Msa0419960:CDS
CTTTGGCAATTTGATTTGCA+GGG 0.562249 3_2:-68162963 Msa0419960:CDS
TTATTGGATCCAAGAGATGA+TGG 0.563781 3_2:+68164196 None:intergenic
TGGTTATACCACAGTTGAAG+AGG 0.565567 3_2:-68163675 Msa0419960:intron
TGTTTATCCTTGCATTGTGA+TGG 0.568663 3_2:-68165805 Msa0419960:CDS
ATCTGATGAGGAATTTGCTA+GGG 0.573680 3_2:-68163935 Msa0419960:CDS
GATCGACAACTAAGATGGAC+AGG 0.574532 3_2:+68165183 None:intergenic
ACCATGAACTTATAAGCCAC+TGG 0.574627 3_2:-68163291 Msa0419960:intron
TCTGGATTCTCATCCCATGA+TGG 0.575349 3_2:+68162248 None:intergenic
ACAACTCATTGAACCATCAT+GGG 0.576187 3_2:-68162261 Msa0419960:CDS
CCTATTTGTTGCAATCACAA+AGG 0.577748 3_2:+68163157 None:intergenic
TATGGGATGCAATTAAACAA+TGG 0.577797 3_2:-68162712 Msa0419960:CDS
AGGACCTGGAGTCACTGGAA+AGG 0.579892 3_2:-68162087 Msa0419960:CDS
CCAAACAATATCCGTATCCA+AGG 0.581077 3_2:-68164768 Msa0419960:CDS
TGTATTAGAATGCTTTCGAC+CGG 0.581752 3_2:+68164735 None:intergenic
ATGAGCTAATAGAACTAAGA+GGG 0.582546 3_2:-68164903 Msa0419960:CDS
TCAAGAGCTTCAAGCATGGT+GGG 0.585631 3_2:-68162636 Msa0419960:CDS
ACCATATGTAAGCAAAGTGA+GGG 0.586108 3_2:-68163521 Msa0419960:CDS
TCCAAGTGCATTTATCTGCA+AGG 0.586289 3_2:+68163079 None:intergenic
AAATGATGGTCTTCTCATAT+GGG 0.587188 3_2:-68162729 Msa0419960:CDS
AGGTTGCTTGAATTTGCGAA+GGG 0.588741 3_2:+68162342 None:intergenic
ACCTTGCAGATAAATGCACT+TGG 0.589225 3_2:-68163080 Msa0419960:CDS
AGATTGGTAGAATCAGAAGC+AGG 0.591256 3_2:+68163364 None:intergenic
CACATTATGAGATTTAGACT+CGG 0.593059 3_2:+68165767 None:intergenic
TTTCTATGTTCCTCGAGATG+AGG 0.593473 3_2:-68164377 Msa0419960:CDS
TTCAATGGAATTGAGTTCTG+TGG 0.597142 3_2:-68162997 Msa0419960:CDS
AAGGTTGCTTGAATTTGCGA+AGG 0.599155 3_2:+68162341 None:intergenic
GTCCATCAGGAAAACCATCA+AGG 0.601868 3_2:+68165205 None:intergenic
TACCATATGTAAGCAAAGTG+AGG 0.603607 3_2:-68163522 Msa0419960:CDS
TACCAAATGATCTTCTCCAC+CGG 0.604794 3_2:-68162938 Msa0419960:intron
TTACCATCACAAACACCAAG+TGG 0.605695 3_2:-68164946 Msa0419960:CDS
ATCAAGAGCTTCAAGCATGG+TGG 0.607474 3_2:-68162637 Msa0419960:CDS
TCAGCAAACGCCTCATCTCG+AGG 0.612448 3_2:+68164367 None:intergenic
TCAATGTGCCTTGTTTGGTG+AGG 0.613172 3_2:+68163455 None:intergenic
GTCTGTGAGAATTGTGTCCA+AGG 0.613851 3_2:+68164282 None:intergenic
GCTTAGTACCTCTTCAACTG+TGG 0.616160 3_2:+68163667 None:intergenic
CTGATCAAGAGCTTCAAGCA+TGG 0.620687 3_2:-68162640 Msa0419960:CDS
TTAGCGACTTTAATCAACCT+GGG 0.622026 3_2:+68164148 None:intergenic
GAAGTTCGAACAAAAGGTCA+CGG 0.622138 3_2:-68162611 Msa0419960:CDS
TAACTTGTAGATTGGATGCA+TGG 0.623713 3_2:+68163124 None:intergenic
TTTGCACAATATGCTTATGG+AGG 0.625959 3_2:-68162479 Msa0419960:CDS
AAATAGCATCCAATTAGTCA+AGG 0.636861 3_2:-68163890 Msa0419960:CDS
TGCAAGGTATATCTGAGATG+TGG 0.649204 3_2:+68163095 None:intergenic
CTCGTGATAGCTGATTCTGG+TGG 0.654841 3_2:+68163722 None:intergenic
GCATCCAATTTACTTCTCAG+AGG 0.654968 3_2:+68163758 None:intergenic
GTTCAAAATGGTCATCACCA+AGG 0.657548 3_2:+68162322 None:intergenic
TTGCTAGGGAGACTTTGGCA+GGG 0.658261 3_2:-68163921 Msa0419960:CDS
TAATGTGAAAGCTTTAGTGA+CGG 0.659359 3_2:-68165751 Msa0419960:CDS
ATGAATGATCGATGAACATG+TGG 0.667532 3_2:+68165951 None:intergenic
TCACCAGATGAAGAGTACTT+GGG 0.668317 3_2:-68162284 Msa0419960:CDS
TGAAAGCTTTAGTGACGGTG+AGG 0.677954 3_2:-68165746 Msa0419960:CDS
TTATTGGCGACAAACCACAA+TGG 0.686808 3_2:-68163402 Msa0419960:CDS
GAAGATCCTAATGCACAACA+TGG 0.692109 3_2:-68162785 Msa0419960:CDS
GCGACAAATCAGAAGAACCA+TGG 0.695121 3_2:-68162589 Msa0419960:CDS
GTGACGGTGAGGCAAAGTGA+TGG 0.699606 3_2:-68165735 Msa0419960:CDS
GCCTACAGAAGACCCTACAA+AGG 0.701209 3_2:-68162417 Msa0419960:CDS
ACGGTGAGGCAAAGTGATGG+TGG 0.702005 3_2:-68165732 Msa0419960:CDS
TAGTGGTGATGATGTCAATG+AGG 0.710309 3_2:+68162540 None:intergenic
GATACAATTCAACACAACCA+CGG 0.729752 3_2:-68164335 Msa0419960:CDS
ACAAATCAGAAGAACCATGG+TGG 0.737932 3_2:-68162586 Msa0419960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAGTAATATAATAAATATT+TGG + chr3_2:68163936-68163955 None:intergenic 10.0%
!! ATTAAAACATAAATAAACCA+TGG + chr3_2:68162392-68162411 None:intergenic 15.0%
!! TCAAAATTATTTATATATGC+TGG + chr3_2:68163278-68163297 None:intergenic 15.0%
!! CAATAATTGTTACAAATATT+TGG - chr3_2:68164082-68164101 Msa0419960:intron 15.0%
!!! TATATGTTGTTGATTTTTAT+AGG - chr3_2:68164744-68164763 Msa0419960:CDS 15.0%
!! CAAATAGTAATTTGAAAAAT+AGG - chr3_2:68165795-68165814 Msa0419960:CDS 15.0%
!!! AGAATATTTTCTCCTTATAT+AGG + chr3_2:68162047-68162066 None:intergenic 20.0%
!! AATTTACTAGTAACAATGAA+TGG + chr3_2:68162466-68162485 None:intergenic 20.0%
!! ATTTGAATAAAATAAACGTG+AGG + chr3_2:68162551-68162570 None:intergenic 20.0%
!!! CGTTTATTTTATTCAAATGA+AGG - chr3_2:68162553-68162572 Msa0419960:CDS 20.0%
!!! GTTTAAGTTTTATACATACA+TGG - chr3_2:68162892-68162911 Msa0419960:intron 20.0%
!! AATAGCATAGAAATTAAACT+TGG + chr3_2:68162949-68162968 None:intergenic 20.0%
!! ATATGTTGTAAATTAGAGAA+AGG + chr3_2:68163320-68163339 None:intergenic 20.0%
!!! CATGAGAATACTTTTTTATT+TGG + chr3_2:68163352-68163371 None:intergenic 20.0%
!!! TTTTACAAGTTATTTGAGTT+GGG - chr3_2:68163956-68163975 Msa0419960:CDS 20.0%
!! TTATATCATCTATGCTAATT+AGG - chr3_2:68164119-68164138 Msa0419960:CDS 20.0%
!!! GAAATCTTTTGTTTTTTTCT+TGG - chr3_2:68164141-68164160 Msa0419960:CDS 20.0%
!! CATATGGTAAATATAAATCA+TGG + chr3_2:68164416-68164435 None:intergenic 20.0%
!!! TTGTTGATTTTTATAGGTTA+AGG - chr3_2:68164750-68164769 Msa0419960:CDS 20.0%
!!! CTTTACTAAAAAGTATTCAA+TGG - chr3_2:68164937-68164956 Msa0419960:CDS 20.0%
!! GTATGATAATAAGTTCAAAA+TGG + chr3_2:68165642-68165661 None:intergenic 20.0%
!! AGTAATTTGAAAAATAGGAA+TGG - chr3_2:68165800-68165819 Msa0419960:CDS 20.0%
!! TACTAAATAGATATGCTATA+TGG + chr3_2:68165893-68165912 None:intergenic 20.0%
!! AAATGTTGCATTAATTGTTA+TGG - chr3_2:68165923-68165942 Msa0419960:CDS 20.0%
!! TTACTTAAAAACAACAACAA+AGG + chr3_2:68165983-68166002 None:intergenic 20.0%
! TAACCAACTATAAGATATCT+TGG - chr3_2:68161935-68161954 Msa0419960:three_prime_UTR 25.0%
! AACCAACTATAAGATATCTT+GGG - chr3_2:68161936-68161955 Msa0419960:three_prime_UTR 25.0%
! TATAAGATATCTTGGGATAT+TGG - chr3_2:68161943-68161962 Msa0419960:three_prime_UTR 25.0%
!! TATTTTCTCCTTATATAGGA+AGG + chr3_2:68162043-68162062 None:intergenic 25.0%
! TAGTACTAGTACAAGTAAAA+AGG - chr3_2:68162120-68162139 Msa0419960:CDS 25.0%
! AATATTGTTACTGGTATTGT+TGG - chr3_2:68162250-68162269 Msa0419960:CDS 25.0%
! GCAATAAAATGAAACTTACT+TGG + chr3_2:68162319-68162338 None:intergenic 25.0%
! AACATAAATAAACCATGGTT+TGG + chr3_2:68162387-68162406 None:intergenic 25.0%
! TATGTATGTACACAAAACTT+TGG - chr3_2:68162436-68162455 Msa0419960:CDS 25.0%
! TTCAAATGAAGGAACAAATT+CGG - chr3_2:68162564-68162583 Msa0419960:CDS 25.0%
!!! CTTGGTCTTGTAAATAAAAT+TGG + chr3_2:68162931-68162950 None:intergenic 25.0%
!!! AAAAACAATTTTGGAATCCT+TGG - chr3_2:68163650-68163669 Msa0419960:intron 25.0%
! TTGGAGCAATGTTAAATTAT+TGG + chr3_2:68163772-68163791 None:intergenic 25.0%
!! GTTTTACAAGTTATTTGAGT+TGG - chr3_2:68163955-68163974 Msa0419960:CDS 25.0%
! GTGTATTCATTATGTTATGT+AGG - chr3_2:68164163-68164182 Msa0419960:CDS 25.0%
! TTCATTATGTTATGTAGGAA+TGG - chr3_2:68164168-68164187 Msa0419960:CDS 25.0%
!!! TGTTTAATTTGAGTATATGC+AGG - chr3_2:68164356-68164375 Msa0419960:CDS 25.0%
! AAACAATTTCTTCTCTTGAA+TGG + chr3_2:68164382-68164401 None:intergenic 25.0%
!! ATCAATTTTTACCTTGATTC+CGG - chr3_2:68164694-68164713 Msa0419960:intron 25.0%
! GAGATTCTTATTAGTTCATA+TGG - chr3_2:68164896-68164915 Msa0419960:CDS 25.0%
! GATATAGTGAGAGAAATTTA+GGG + chr3_2:68165099-68165118 None:intergenic 25.0%
! TGATATAGTGAGAGAAATTT+AGG + chr3_2:68165100-68165119 None:intergenic 25.0%
!! TCTTTTTGTGTTTTCAGATT+TGG + chr3_2:68165389-68165408 None:intergenic 25.0%
! ATATAAAGGTAGCATTTGAA+AGG - chr3_2:68165723-68165742 Msa0419960:CDS 25.0%
! TAGCATTTGAAAGGTTTAAT+GGG - chr3_2:68165732-68165751 Msa0419960:CDS 25.0%
! TGCATTAATTGTTATGGAAT+AGG - chr3_2:68165929-68165948 Msa0419960:CDS 25.0%
!! TTTTTCTTGAATCTCTTTGA+TGG - chr3_2:68166023-68166042 Msa0419960:five_prime_UTR 25.0%
AAATTGCATTTATCTCCTCT+GGG + chr3_2:68161901-68161920 None:intergenic 30.0%
TAAATTGCATTTATCTCCTC+TGG + chr3_2:68161902-68161921 None:intergenic 30.0%
ATCCCAAGATATCTTATAGT+TGG + chr3_2:68161941-68161960 None:intergenic 30.0%
GTTGCTACAACAAAAGTAAT+TGG - chr3_2:68162071-68162090 Msa0419960:CDS 30.0%
CACATTATGAGATTTAGACT+CGG + chr3_2:68162185-68162204 None:intergenic 30.0%
! TAATGTGAAAGCTTTAGTGA+CGG - chr3_2:68162198-68162217 Msa0419960:CDS 30.0%
CTCATCAAGAATATTGTTAC+TGG - chr3_2:68162241-68162260 Msa0419960:CDS 30.0%
!! TGGTAACAAACACTTGTTTT+TGG - chr3_2:68162270-68162289 Msa0419960:CDS 30.0%
ATATGAACACAATTCTTCGA+AGG - chr3_2:68162516-68162535 Msa0419960:CDS 30.0%
ATAAAATAAACGTGAGGTCT+AGG + chr3_2:68162545-68162564 None:intergenic 30.0%
TCGGAGAAAAAGACAATAAA+AGG - chr3_2:68162583-68162602 Msa0419960:CDS 30.0%
AACTGAGAAAAAAGAGAATG+AGG - chr3_2:68162618-68162637 Msa0419960:CDS 30.0%
CTAAATAACATAGTCCTTGA+TGG - chr3_2:68162730-68162749 Msa0419960:CDS 30.0%
ATGAGCTAATAGAACTAAGA+GGG - chr3_2:68163046-68163065 Msa0419960:CDS 30.0%
TATGATGTCTACAATGATCT+TGG - chr3_2:68163111-68163130 Msa0419960:CDS 30.0%
!!! AGGTCTTTTGAGTTTAATGT+TGG + chr3_2:68163148-68163167 None:intergenic 30.0%
CTGGACAACTAAAATGAAAT+AGG + chr3_2:68163259-68163278 None:intergenic 30.0%
ATCATACGAAAAGAGAGATT+CGG - chr3_2:68163548-68163567 Msa0419960:CDS 30.0%
!!! CAAAATTGTTTTTACTCCTG+AGG + chr3_2:68163643-68163662 None:intergenic 30.0%
! CTCAGGAGTAAAAACAATTT+TGG - chr3_2:68163641-68163660 Msa0419960:intron 30.0%
ATTCTCACAGACATAAATCT+TGG - chr3_2:68163678-68163697 Msa0419960:CDS 30.0%
ATAGACGAACTTTACAAAGA+AGG - chr3_2:68163720-68163739 Msa0419960:CDS 30.0%
TTTCAATTACCATCATCTCT+TGG - chr3_2:68163744-68163763 Msa0419960:CDS 30.0%
! TGACTAATTGGATGCTATTT+GGG + chr3_2:68164059-68164078 None:intergenic 30.0%
TTGACTAATTGGATGCTATT+TGG + chr3_2:68164060-68164079 None:intergenic 30.0%
AAATAGCATCCAATTAGTCA+AGG - chr3_2:68164059-68164078 Msa0419960:intron 30.0%
CAATTATTGCCTTGACTAAT+TGG + chr3_2:68164071-68164090 None:intergenic 30.0%
AAATTGGATGCCCAAATTTA+TGG - chr3_2:68164203-68164222 Msa0419960:CDS 30.0%
ATGAAGAATACTACACTGTA+TGG - chr3_2:68164453-68164472 Msa0419960:intron 30.0%
GTGAATATTTGTTTCCATTG+TGG + chr3_2:68164564-68164583 None:intergenic 30.0%
! CTATTTGTTGCAATCACAAA+GGG + chr3_2:68164794-68164813 None:intergenic 30.0%
GAAGAAATTCATGATACGTA+TGG + chr3_2:68165043-68165062 None:intergenic 30.0%
!! GCAAATTGTTGTTCTTTTAG+AGG - chr3_2:68165131-68165150 Msa0419960:CDS 30.0%
!! TTGTTGTTCTTTTAGAGGAA+TGG - chr3_2:68165136-68165155 Msa0419960:CDS 30.0%
AAATGATGGTCTTCTCATAT+GGG - chr3_2:68165220-68165239 Msa0419960:CDS 30.0%
TATGGGATGCAATTAAACAA+TGG - chr3_2:68165237-68165256 Msa0419960:CDS 30.0%
ATGGGATGCAATTAAACAAT+GGG - chr3_2:68165238-68165257 Msa0419960:CDS 30.0%
AACTTTGCACAATATGCTTA+TGG - chr3_2:68165467-68165486 Msa0419960:intron 30.0%
TAGCAATTGTAGGTCTATTA+GGG + chr3_2:68165502-68165521 None:intergenic 30.0%
!! ATGTTGTTTCTAGCAATTGT+AGG + chr3_2:68165512-68165531 None:intergenic 30.0%
TCAAATGCTACCTTTATATC+TGG + chr3_2:68165722-68165741 None:intergenic 30.0%
GTAGCATTTGAAAGGTTTAA+TGG - chr3_2:68165731-68165750 Msa0419960:CDS 30.0%
!! TCAACAAAAGCGTCATAGTT+TGG + chr3_2:68161973-68161992 None:intergenic 35.0%
ATGAATGATCGATGAACATG+TGG + chr3_2:68162001-68162020 None:intergenic 35.0%
CATCTTCACCTTCCTATATA+AGG - chr3_2:68162032-68162051 Msa0419960:three_prime_UTR 35.0%
TGTTTATCCTTGCATTGTGA+TGG - chr3_2:68162144-68162163 Msa0419960:CDS 35.0%
GATGTTACTGTAAAGGACAA+TGG + chr3_2:68162365-68162384 None:intergenic 35.0%
! TGGTTTGGATGTTACTGTAA+AGG + chr3_2:68162372-68162391 None:intergenic 35.0%
TTACAGTAACATCCAAACCA+TGG - chr3_2:68162372-68162391 Msa0419960:CDS 35.0%
!! TTTGAGTTACCAGCAGATTT+TGG - chr3_2:68162658-68162677 Msa0419960:CDS 35.0%
!! TTTGTAGGAGTATCATGCTT+GGG + chr3_2:68162802-68162821 None:intergenic 35.0%
GTGAAGAAAACTCTCTTTGT+AGG + chr3_2:68162817-68162836 None:intergenic 35.0%
! GAGAGTTTTCTTCACTAACA+AGG - chr3_2:68162822-68162841 Msa0419960:intron 35.0%
CTAAGCTTTCTTAATCCACT+TGG + chr3_2:68163021-68163040 None:intergenic 35.0%
GATGAGCTAATAGAACTAAG+AGG - chr3_2:68163045-68163064 Msa0419960:CDS 35.0%
CTAATAGAACTAAGAGGGAA+TGG - chr3_2:68163051-68163070 Msa0419960:CDS 35.0%
! TTGAGTTTAATGTTGGTCTC+AGG + chr3_2:68163141-68163160 None:intergenic 35.0%
AAACTCAAAAGACCTGTTCT+TGG - chr3_2:68163153-68163172 Msa0419960:CDS 35.0%
TGTATTAGAATGCTTTCGAC+CGG + chr3_2:68163217-68163236 None:intergenic 35.0%
! GAATCTCTCTTTTCGTATGA+TGG + chr3_2:68163549-68163568 None:intergenic 35.0%
GATACAATTCAACACAACCA+CGG - chr3_2:68163614-68163633 Msa0419960:intron 35.0%
TTATTGGATCCAAGAGATGA+TGG + chr3_2:68163756-68163775 None:intergenic 35.0%
TTAGCGACTTTAATCAACCT+GGG + chr3_2:68163804-68163823 None:intergenic 35.0%
ATTAGCGACTTTAATCAACC+TGG + chr3_2:68163805-68163824 None:intergenic 35.0%
! TATCAAAGCGCAAAAGGTTT+TGG + chr3_2:68163833-68163852 None:intergenic 35.0%
!! CTTACTTTTAAGTGCTTCAG+GGG + chr3_2:68163856-68163875 None:intergenic 35.0%
!! GCTTACTTTTAAGTGCTTCA+GGG + chr3_2:68163857-68163876 None:intergenic 35.0%
!! TGCTTACTTTTAAGTGCTTC+AGG + chr3_2:68163858-68163877 None:intergenic 35.0%
! TTGCAGGAGACAAATTCTTT+TGG - chr3_2:68163989-68164008 Msa0419960:intron 35.0%
!! ATTCTTTTGGCTATCTGATG+AGG - chr3_2:68164002-68164021 Msa0419960:intron 35.0%
! TATCTGATGAGGAATTTGCT+AGG - chr3_2:68164013-68164032 Msa0419960:intron 35.0%
!! ATCTGATGAGGAATTTGCTA+GGG - chr3_2:68164014-68164033 Msa0419960:intron 35.0%
! TAATTGGATGCTATTTGGGT+TGG + chr3_2:68164055-68164074 None:intergenic 35.0%
! TTCTGGTGGACCATAAATTT+GGG + chr3_2:68164216-68164235 None:intergenic 35.0%
! ATTCTGGTGGACCATAAATT+TGG + chr3_2:68164217-68164236 None:intergenic 35.0%
TACCATATGTAAGCAAAGTG+AGG - chr3_2:68164427-68164446 Msa0419960:intron 35.0%
ACCATATGTAAGCAAAGTGA+GGG - chr3_2:68164428-68164447 Msa0419960:intron 35.0%
CTTCTGATTCTACCAATCTT+TGG - chr3_2:68164589-68164608 Msa0419960:intron 35.0%
! ATTCTACCAATCTTTGGCTT+TGG - chr3_2:68164595-68164614 Msa0419960:intron 35.0%
CATGTTCTTACTCATGATAC+TGG - chr3_2:68164633-68164652 Msa0419960:intron 35.0%
! CCTATTTGTTGCAATCACAA+AGG + chr3_2:68164795-68164814 None:intergenic 35.0%
CCTTTGTGATTGCAACAAAT+AGG - chr3_2:68164792-68164811 Msa0419960:CDS 35.0%
! TAACTTGTAGATTGGATGCA+TGG + chr3_2:68164828-68164847 None:intergenic 35.0%
TTCAATGGAATTGAGTTCTG+TGG - chr3_2:68164952-68164971 Msa0419960:CDS 35.0%
!! CTTTGGCAATTTGATTTGCA+GGG - chr3_2:68164986-68165005 Msa0419960:intron 35.0%
ATGATACGTATGGACTTTAC+CGG + chr3_2:68165033-68165052 None:intergenic 35.0%
ATAGTGAGAGAAATTTAGGG+AGG + chr3_2:68165096-68165115 None:intergenic 35.0%
!! AGTCTTCTATTGCTAGCTTT+AGG + chr3_2:68165190-68165209 None:intergenic 35.0%
GAAGACCATCATTTGCAAAA+GGG + chr3_2:68165214-68165233 None:intergenic 35.0%
AGAAGACCATCATTTGCAAA+AGG + chr3_2:68165215-68165234 None:intergenic 35.0%
CAAATGATGGTCTTCTCATA+TGG - chr3_2:68165219-68165238 Msa0419960:CDS 35.0%
GATTCAATGATGCTTGGATT+TGG + chr3_2:68165290-68165309 None:intergenic 35.0%
TGATCAGATTCAATGATGCT+TGG + chr3_2:68165296-68165315 None:intergenic 35.0%
TTTGCACAATATGCTTATGG+AGG - chr3_2:68165470-68165489 Msa0419960:intron 35.0%
CTAGCAATTGTAGGTCTATT+AGG + chr3_2:68165503-68165522 None:intergenic 35.0%
ACAACTCATTGAACCATCAT+GGG - chr3_2:68165688-68165707 Msa0419960:CDS 35.0%
GGATGAGAATCCAGATATAA+AGG - chr3_2:68165709-68165728 Msa0419960:CDS 35.0%
AAGGTTTAATGGGAGATTGA+AGG - chr3_2:68165742-68165761 Msa0419960:CDS 35.0%
TTGAAAAATAGGAATGGAGC+TGG - chr3_2:68165806-68165825 Msa0419960:CDS 35.0%
AATGGCTTCATTAGCTCATA+AGG + chr3_2:68165839-68165858 None:intergenic 35.0%
TTTGTTGCCATCACAATGCA+AGG + chr3_2:68162154-68162173 None:intergenic 40.0%
!!! CAAACACTTGTTTTTGGAGC+TGG - chr3_2:68162276-68162295 Msa0419960:CDS 40.0%
AGAGAATGAGGTGCAATATG+AGG - chr3_2:68162630-68162649 Msa0419960:CDS 40.0%
!! ACCAGCAGATTTTGGAAATG+TGG - chr3_2:68162666-68162685 Msa0419960:CDS 40.0%
!! CCAGCAGATTTTGGAAATGT+GGG - chr3_2:68162667-68162686 Msa0419960:CDS 40.0%
TCCATCTTAGTTGTCGATCA+TGG - chr3_2:68162770-68162789 Msa0419960:CDS 40.0%
TCCATGATCGACAACTAAGA+TGG + chr3_2:68162774-68162793 None:intergenic 40.0%
!! CTTTGTAGGAGTATCATGCT+TGG + chr3_2:68162803-68162822 None:intergenic 40.0%
TTACCATCACAAACACCAAG+TGG - chr3_2:68163003-68163022 Msa0419960:CDS 40.0%
!! AATCCACTTGGTGTTTGTGA+TGG + chr3_2:68163009-68163028 None:intergenic 40.0%
CCTTGGATACGGATATTGTT+TGG + chr3_2:68163184-68163203 None:intergenic 40.0%
CCAAACAATATCCGTATCCA+AGG - chr3_2:68163181-68163200 Msa0419960:CDS 40.0%
!! GGTCGAAAGCATTCTAATAC+AGG - chr3_2:68163216-68163235 Msa0419960:intron 40.0%
TTTCTATGTTCCTCGAGATG+AGG - chr3_2:68163572-68163591 Msa0419960:CDS 40.0%
GACTTTAATCAACCTGGGAA+TGG + chr3_2:68163799-68163818 None:intergenic 40.0%
! ATGGCCTCTGAGAAGTAAAT+TGG - chr3_2:68164187-68164206 Msa0419960:CDS 40.0%
GCATCCAATTTACTTCTCAG+AGG + chr3_2:68164194-68164213 None:intergenic 40.0%
TGGTTATACCACAGTTGAAG+AGG - chr3_2:68164274-68164293 Msa0419960:CDS 40.0%
TCCCTCACTTTGCTTACATA+TGG + chr3_2:68164432-68164451 None:intergenic 40.0%
TTGTTCTTCCTCACCAAACA+AGG - chr3_2:68164486-68164505 Msa0419960:intron 40.0%
CTAGTGAGCTCAATGACTAT+TGG + chr3_2:68164522-68164541 None:intergenic 40.0%
! TGTGGTTTGTCGCCAATAAT+CGG + chr3_2:68164546-68164565 None:intergenic 40.0%
TTATTGGCGACAAACCACAA+TGG - chr3_2:68164547-68164566 Msa0419960:intron 40.0%
AGATTGGTAGAATCAGAAGC+AGG + chr3_2:68164588-68164607 None:intergenic 40.0%
ACCATGAACTTATAAGCCAC+TGG - chr3_2:68164658-68164677 Msa0419960:intron 40.0%
ACCAGTGGCTTATAAGTTCA+TGG + chr3_2:68164662-68164681 None:intergenic 40.0%
GATATGAATGTACTCACCAG+TGG + chr3_2:68164677-68164696 None:intergenic 40.0%
TCATATACTCACCGGAATCA+AGG + chr3_2:68164708-68164727 None:intergenic 40.0%
GCACTACTTCATATACTCAC+CGG + chr3_2:68164716-68164735 None:intergenic 40.0%
! GGATGCAGTAACTTGTAGAT+TGG + chr3_2:68164836-68164855 None:intergenic 40.0%
! TGCAAGGTATATCTGAGATG+TGG + chr3_2:68164857-68164876 None:intergenic 40.0%
ACCTTGCAGATAAATGCACT+TGG - chr3_2:68164869-68164888 Msa0419960:CDS 40.0%
TCCAAGTGCATTTATCTGCA+AGG + chr3_2:68164873-68164892 None:intergenic 40.0%
CTGTGGCATATGATAAGCTT+TGG - chr3_2:68164969-68164988 Msa0419960:intron 40.0%
!! GCTTTGGCAATTTGATTTGC+AGG - chr3_2:68164985-68165004 Msa0419960:intron 40.0%
TACCAAATGATCTTCTCCAC+CGG - chr3_2:68165011-68165030 Msa0419960:intron 40.0%
!! TTCTTTTAGAGGAATGGCTG+TGG - chr3_2:68165142-68165161 Msa0419960:CDS 40.0%
GAAGATCCTAATGCACAACA+TGG - chr3_2:68165164-68165183 Msa0419960:CDS 40.0%
TTTAGGCCATGTTGTGCATT+AGG + chr3_2:68165173-68165192 None:intergenic 40.0%
! GACTACCCTTTTGCAAATGA+TGG - chr3_2:68165206-68165225 Msa0419960:CDS 40.0%
GAAGTTCGAACAAAAGGTCA+CGG - chr3_2:68165338-68165357 Msa0419960:CDS 40.0%
ACAAATCAGAAGAACCATGG+TGG - chr3_2:68165363-68165382 Msa0419960:CDS 40.0%
! TAGTGGTGATGATGTCAATG+AGG + chr3_2:68165412-68165431 None:intergenic 40.0%
ACATCATCACCACTATAGCT+TGG - chr3_2:68165417-68165436 Msa0419960:intron 40.0%
CATCATCACCACTATAGCTT+GGG - chr3_2:68165418-68165437 Msa0419960:intron 40.0%
AAGACCCTACAAAGGAAGAA+TGG - chr3_2:68165540-68165559 Msa0419960:intron 40.0%
AGACCCTACAAAGGAAGAAT+GGG - chr3_2:68165541-68165560 Msa0419960:intron 40.0%
TCTCCCATTCTTCCTTTGTA+GGG + chr3_2:68165547-68165566 None:intergenic 40.0%
TTCTCCCATTCTTCCTTTGT+AGG + chr3_2:68165548-68165567 None:intergenic 40.0%
! CAAACCTGAGCAAACACTTT+TGG - chr3_2:68165577-68165596 Msa0419960:intron 40.0%
AGGTTGCTTGAATTTGCGAA+GGG + chr3_2:68165610-68165629 None:intergenic 40.0%
AAGGTTGCTTGAATTTGCGA+AGG + chr3_2:68165611-68165630 None:intergenic 40.0%
TTCGCAAATTCAAGCAACCT+TGG - chr3_2:68165610-68165629 Msa0419960:intron 40.0%
GTTCAAAATGGTCATCACCA+AGG + chr3_2:68165630-68165649 None:intergenic 40.0%
AGTACTCTTCATCTGGTGAA+TGG + chr3_2:68165664-68165683 None:intergenic 40.0%
!! TTCACCAGATGAAGAGTACT+TGG - chr3_2:68165664-68165683 Msa0419960:CDS 40.0%
!! TCACCAGATGAAGAGTACTT+GGG - chr3_2:68165665-68165684 Msa0419960:CDS 40.0%
GACAACTCATTGAACCATCA+TGG - chr3_2:68165687-68165706 Msa0419960:CDS 40.0%
GGGAGATTGAAGGAAATTGA+AGG - chr3_2:68165752-68165771 Msa0419960:CDS 40.0%
!! GAGCTAATGAAGCCATTTTC+AGG - chr3_2:68165842-68165861 Msa0419960:CDS 40.0%
! TGAAAGCTTTAGTGACGGTG+AGG - chr3_2:68162203-68162222 Msa0419960:CDS 45.0%
GGATATCATGCACATGTGTG+TGG + chr3_2:68162344-68162363 None:intergenic 45.0%
CCCACATTTCCAAAATCTGC+TGG + chr3_2:68162670-68162689 None:intergenic 45.0%
!! GTCCTTGATGGTTTTCCTGA+TGG - chr3_2:68162742-68162761 Msa0419960:CDS 45.0%
GTCCATCAGGAAAACCATCA+AGG + chr3_2:68162747-68162766 None:intergenic 45.0%
GATCGACAACTAAGATGGAC+AGG + chr3_2:68162769-68162788 None:intergenic 45.0%
GAACTAAGAGGGAATGGAGA+AGG - chr3_2:68163057-68163076 Msa0419960:CDS 45.0%
CTCAAAAGACCTGTTCTTGG+TGG - chr3_2:68163156-68163175 Msa0419960:CDS 45.0%
! TGAGGCGTTTGCTGAGATAA+AGG - chr3_2:68163590-68163609 Msa0419960:intron 45.0%
GTCTGTGAGAATTGTGTCCA+AGG + chr3_2:68163670-68163689 None:intergenic 45.0%
! TCAACCTGGGAATGGCTTTT+TGG + chr3_2:68163791-68163810 None:intergenic 45.0%
GTTGGGAACTTCTGAATTGC+AGG - chr3_2:68163973-68163992 Msa0419960:intron 45.0%
!! GGAATTTGCTAGGGAGACTT+TGG - chr3_2:68164023-68164042 Msa0419960:intron 45.0%
! TCTCTCGTGATAGCTGATTC+TGG + chr3_2:68164233-68164252 None:intergenic 45.0%
GTCATCGAGTCGCAGATAAT+TGG - chr3_2:68164254-68164273 Msa0419960:CDS 45.0%
GCTTAGTACCTCTTCAACTG+TGG + chr3_2:68164285-68164304 None:intergenic 45.0%
!! TCAATGTGCCTTGTTTGGTG+AGG + chr3_2:68164497-68164516 None:intergenic 45.0%
! TGGCTTCAATGTGCCTTGTT+TGG + chr3_2:68164502-68164521 None:intergenic 45.0%
GAGCTCACTAGACCGATTAT+TGG - chr3_2:68164531-68164550 Msa0419960:intron 45.0%
!! CTACCAATCTTTGGCTTTGG+AGG - chr3_2:68164598-68164617 Msa0419960:intron 45.0%
AAGCCTCCAAAGCCAAAGAT+TGG + chr3_2:68164604-68164623 None:intergenic 45.0%
TACCGGTGGAGAAGATCATT+TGG + chr3_2:68165016-68165035 None:intergenic 45.0%
ATACGTATGGACTTTACCGG+TGG + chr3_2:68165030-68165049 None:intergenic 45.0%
CTGATCAAGAGCTTCAAGCA+TGG - chr3_2:68165309-68165328 Msa0419960:CDS 45.0%
ATCAAGAGCTTCAAGCATGG+TGG - chr3_2:68165312-68165331 Msa0419960:CDS 45.0%
TCAAGAGCTTCAAGCATGGT+GGG - chr3_2:68165313-68165332 Msa0419960:CDS 45.0%
TGGGCAGAAGTTCGAACAAA+AGG - chr3_2:68165332-68165351 Msa0419960:CDS 45.0%
GCGACAAATCAGAAGAACCA+TGG - chr3_2:68165360-68165379 Msa0419960:CDS 45.0%
! GTTTTCAGATTTGGCCACCA+TGG + chr3_2:68165380-68165399 None:intergenic 45.0%
AGATGCTACCCAAGCTATAG+TGG + chr3_2:68165429-68165448 None:intergenic 45.0%
TCCTTTGTAGGGTCTTCTGT+AGG + chr3_2:68165536-68165555 None:intergenic 45.0%
!! CACTCCAAAAGTGTTTGCTC+AGG + chr3_2:68165584-68165603 None:intergenic 45.0%
TGTCCCAAGTACTCTTCATC+TGG + chr3_2:68165671-68165690 None:intergenic 45.0%
TCTGGATTCTCATCCCATGA+TGG + chr3_2:68165704-68165723 None:intergenic 45.0%
!! ATGAAGCCATTTTCAGGACC+TGG - chr3_2:68165848-68165867 Msa0419960:CDS 45.0%
!! TGGAAATGTGGGCGCTGTTT+TGG - chr3_2:68162678-68162697 Msa0419960:CDS 50.0%
CTAAGATGGACAGGTCCATC+AGG + chr3_2:68162760-68162779 None:intergenic 50.0%
!! TGTTTGGTGCCACCAAGAAC+AGG + chr3_2:68163168-68163187 None:intergenic 50.0%
GTACGACAACGCCTTGGATA+CGG + chr3_2:68163195-68163214 None:intergenic 50.0%
!! TATCCAAGGCGTTGTCGTAC+CGG - chr3_2:68163195-68163214 Msa0419960:intron 50.0%
TGCTCCAAAAAGCCATTCCC+AGG - chr3_2:68163784-68163803 Msa0419960:intron 50.0%
!! CAGGGGTATCAAAGCGCAAA+AGG + chr3_2:68163839-68163858 None:intergenic 50.0%
TTTGCTAGGGAGACTTTGGC+AGG - chr3_2:68164027-68164046 Msa0419960:intron 50.0%
TTGCTAGGGAGACTTTGGCA+GGG - chr3_2:68164028-68164047 Msa0419960:intron 50.0%
! CTCGTGATAGCTGATTCTGG+TGG + chr3_2:68164230-68164249 None:intergenic 50.0%
GCCTACAGAAGACCCTACAA+AGG - chr3_2:68165532-68165551 Msa0419960:intron 50.0%
GTGACTCCAGGTCCTGAAAA+TGG + chr3_2:68165857-68165876 None:intergenic 50.0%
!! TTTTCAGGACCTGGAGTCAC+TGG - chr3_2:68165857-68165876 Msa0419960:CDS 50.0%
GTGACGGTGAGGCAAAGTGA+TGG - chr3_2:68162214-68162233 Msa0419960:CDS 55.0%
ACGGTGAGGCAAAGTGATGG+TGG - chr3_2:68162217-68162236 Msa0419960:CDS 55.0%
TCAGCAAACGCCTCATCTCG+AGG + chr3_2:68163585-68163604 None:intergenic 55.0%
! TTTTACTCCTGAGGACGCCG+TGG + chr3_2:68163634-68163653 None:intergenic 55.0%
TGCTAGGGAGACTTTGGCAG+GGG - chr3_2:68164029-68164048 Msa0419960:intron 55.0%
AGGACCTGGAGTCACTGGAA+AGG - chr3_2:68165862-68165881 Msa0419960:CDS 55.0%
GGACCTGGAGTCACTGGAAA+GGG - chr3_2:68165863-68165882 Msa0419960:CDS 55.0%
ACTCCCTTTCCAGTGACTCC+AGG + chr3_2:68165869-68165888 None:intergenic 55.0%
AACACAACCACGGCGTCCTC+AGG - chr3_2:68163624-68163643 Msa0419960:intron 60.0%
CGACCGGTACGACAACGCCT+TGG + chr3_2:68163201-68163220 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 68161888 68166083 68161888 ID=Msa0419960;Name=Msa0419960
chr3_2 mRNA 68161888 68166083 68161888 ID=Msa0419960-mRNA-1;Parent=Msa0419960;Name=Msa0419960-mRNA-1;_AED=0.09;_eAED=0.09;_QI=89|0.87|0.88|1|1|1|9|173|904
chr3_2 exon 68161888 68162820 68161888 ID=Msa0419960-mRNA-1:exon:16024;Parent=Msa0419960-mRNA-1
chr3_2 exon 68162939 68163205 68162939 ID=Msa0419960-mRNA-1:exon:16023;Parent=Msa0419960-mRNA-1
chr3_2 exon 68163292 68163593 68163292 ID=Msa0419960-mRNA-1:exon:16022;Parent=Msa0419960-mRNA-1
chr3_2 exon 68163676 68163786 68163676 ID=Msa0419960-mRNA-1:exon:16021;Parent=Msa0419960-mRNA-1
chr3_2 exon 68163882 68163976 68163882 ID=Msa0419960-mRNA-1:exon:16020;Parent=Msa0419960-mRNA-1
chr3_2 exon 68164101 68164427 68164101 ID=Msa0419960-mRNA-1:exon:16019;Parent=Msa0419960-mRNA-1
chr3_2 exon 68164734 68164974 68164734 ID=Msa0419960-mRNA-1:exon:16018;Parent=Msa0419960-mRNA-1
chr3_2 exon 68165128 68165396 68165128 ID=Msa0419960-mRNA-1:exon:16017;Parent=Msa0419960-mRNA-1
chr3_2 exon 68165652 68166083 68165652 ID=Msa0419960-mRNA-1:exon:16016;Parent=Msa0419960-mRNA-1
chr3_2 five_prime_UTR 68165995 68166083 68165995 ID=Msa0419960-mRNA-1:five_prime_utr;Parent=Msa0419960-mRNA-1
chr3_2 CDS 68165652 68165994 68165652 ID=Msa0419960-mRNA-1:cds;Parent=Msa0419960-mRNA-1
chr3_2 CDS 68165128 68165396 68165128 ID=Msa0419960-mRNA-1:cds;Parent=Msa0419960-mRNA-1
chr3_2 CDS 68164734 68164974 68164734 ID=Msa0419960-mRNA-1:cds;Parent=Msa0419960-mRNA-1
chr3_2 CDS 68164101 68164427 68164101 ID=Msa0419960-mRNA-1:cds;Parent=Msa0419960-mRNA-1
chr3_2 CDS 68163882 68163976 68163882 ID=Msa0419960-mRNA-1:cds;Parent=Msa0419960-mRNA-1
chr3_2 CDS 68163676 68163786 68163676 ID=Msa0419960-mRNA-1:cds;Parent=Msa0419960-mRNA-1
chr3_2 CDS 68163292 68163593 68163292 ID=Msa0419960-mRNA-1:cds;Parent=Msa0419960-mRNA-1
chr3_2 CDS 68162939 68163205 68162939 ID=Msa0419960-mRNA-1:cds;Parent=Msa0419960-mRNA-1
chr3_2 CDS 68162061 68162820 68162061 ID=Msa0419960-mRNA-1:cds;Parent=Msa0419960-mRNA-1
chr3_2 three_prime_UTR 68161888 68162060 68161888 ID=Msa0419960-mRNA-1:three_prime_utr;Parent=Msa0419960-mRNA-1
Gene Sequence

>Msa0419960

ATGACGCTTTTGTTGAAGAAGCCACATGTTCATCGATCATTCATCATTAACAATGCATCTTCACCTTCCTATATAAGGAGAAAATATTCTCATAGTTGCTACAACAAAAGTAATTGGTTCTTGAAATCTTCTTTGTCTCTAATTAGTACTAGTACAAGTAAAAAGGTTGTTTATCCTTGCATTGTGATGGCAACAAAAAGTAGCACCGAGTCTAAATCTCATAATGTGAAAGCTTTAGTGACGGTGAGGCAAAGTGATGGTGGTCTCATCAAGAATATTGTTACTGGTATTGTTGGTAACAAACACTTGTTTTTGGAGCTGGTTAGTGCTGAACTTGATCCAAGAACAAATTCGGAGAAAAAGACAATAAAAGGAAAAGCGCACGAAACTGAGAAAAAAGAGAATGAGGTGCAATATGAGGCAACATTTGAGTTACCAGCAGATTTTGGAAATGTGGGCGCTGTTTTGGTTGAGAATGAGCATGACAAAGAGATATTTCTAAATAACATAGTCCTTGATGGTTTTCCTGATGGACCTGTCCATCTTAGTTGTCGATCATGGATTCAACCCAAGCATGATACTCCTACAAAGAGAGTTTTCTTCACTAACAAGATGTATTTACCATCACAAACACCAAGTGGATTAAGAAAGCTTAGAGAAGATGAGCTAATAGAACTAAGAGGGAATGGAGAAGGAGAAAGAAAAAGCTCAGATAGAATTTATGACTATGATGTCTACAATGATCTTGGAGATCCTGAGACCAACATTAAACTCAAAAGACCTGTTCTTGGTGGCACCAAACAATATCCGTATCCAAGGCGTTGTCGTACCGGTCGAAAGCATTCTAATACAGATCCATCATACGAAAAGAGAGATTCGGATTTCTATGTTCCTCGAGATGAGGCGTTTGCTGAGATAAAGGAGATACAATTCAACACAACCACGGCGTCCTCAGGAGTAAAAACAATTTTGGAATCCTTGGACACAATTCTCACAGACATAAATCTTGGATTTGACAGCTTTGAAGACATAGACGAACTTTACAAAGAAGGATTTCAATTACCATCATCTCTTGGATCCAATAATTTAACATTGCTCCAAAAAGCCATTCCCAGGTTGATTAAAGTCGCTAATGACAGCCAAAACCTTTTGCGCTTTGATACCCCTGAAGCACTTAAAAGAGACAAATTCTTTTGGCTATCTGATGAGGAATTTGCTAGGGAGACTTTGGCAGGGGCCAACCCAAATAGCATCCAATTAGTCAAGGCAATAATTGAATGGCCTCTGAGAAGTAAATTGGATGCCCAAATTTATGGTCCACCAGAATCAGCTATCACGAGAGAAGTCATCGAGTCGCAGATAATTGGTTATACCACAGTTGAAGAGGCCATTCAAGAGAAGAAATTGTTTGTACTAGACTACCATGATTTATATTTACCATATGTAAGCAAAGTGAGGGAAATGAAGAATACTACACTGTATGGATCAAGAACATTGTTCTTCCTCACCAAACAAGGCACATTGAAGCCAATAGTCATTGAGCTCACTAGACCGATTATTGGCGACAAACCACAATGGAAACAAATATTCACTCCTGCTTCTGATTCTACCAATCTTTGGCTTTGGAGGCTTGCAAAAGCTCATGTTCTTACTCATGATACTGGTTACCATGAACTTATAAGCCACTGGTTAAGGACTCACTGTGTTGTAGAACCCTTTGTGATTGCAACAAATAGGCAACTTAGCACCATGCATCCAATCTACAAGTTACTGCATCCACATCTCAGATATACCTTGCAGATAAATGCACTTGGACGCGAGATTCTTATTAGTTCATATGGAGTCATTGAAAGCACATTCTTTACTAAAAAGTATTCAATGGAATTGAGTTCTGTGGCATATGATAAGCTTTGGCAATTTGATTTGCAGGGACTACCAAATGATCTTCTCCACCGAGGAATGGCTGTGGAAGATCCTAATGCACAACATGGCCTAAAGCTAGCAATAGAAGACTACCCTTTTGCAAATGATGGTCTTCTCATATGGGATGCAATTAAACAATGGGTGACAGATTATGTCAATCATTACTATCCAAATCCAAGCATCATTGAATCTGATCAAGAGCTTCAAGCATGGTGGGCAGAAGTTCGAACAAAAGGTCACGGCGACAAATCAGAAGAACCATGGTGGCCAAATCTGAAAACACAAAAAGACCTCATTGACATCATCACCACTATAGCTTGGGTAGCATCTGCACATCATTCAGCTACAAACTTTGCACAATATGCTTATGGAGGCTATTTCCCTAATAGACCTACAATTGCTAGAAACAACATGCCTACAGAAGACCCTACAAAGGAAGAATGGGAGAAATTTCTGAACAAACCTGAGCAAACACTTTTGGAGTGTTTCCCTTCGCAAATTCAAGCAACCTTGGTGATGACCATTTTGAACTTATTATCATACCATTCACCAGATGAAGAGTACTTGGGACAACTCATTGAACCATCATGGGATGAGAATCCAGATATAAAGGTAGCATTTGAAAGGTTTAATGGGAGATTGAAGGAAATTGAAGGTATAATAGACTCAAGAAATGCAAATAGTAATTTGAAAAATAGGAATGGAGCTGGAATAGTGCCTTATGAGCTAATGAAGCCATTTTCAGGACCTGGAGTCACTGGAAAGGGAGTTCCATATAGCATATCTATTTAG
Protein sequence

>Msa0419960

MTLLLKKPHVHRSFIINNASSPSYIRRKYSHSCYNKSNWFLKSSLSLISTSTSKKVVYPCIVMATKSSTESKSHNVKALVTVRQSDGGLIKNIVTGIVGNKHLFLELVSAELDPRTNSEKKTIKGKAHETEKKENEVQYEATFELPADFGNVGAVLVENEHDKEIFLNNIVLDGFPDGPVHLSCRSWIQPKHDTPTKRVFFTNKMYLPSQTPSGLRKLREDELIELRGNGEGERKSSDRIYDYDVYNDLGDPETNIKLKRPVLGGTKQYPYPRRCRTGRKHSNTDPSYEKRDSDFYVPRDEAFAEIKEIQFNTTTASSGVKTILESLDTILTDINLGFDSFEDIDELYKEGFQLPSSLGSNNLTLLQKAIPRLIKVANDSQNLLRFDTPEALKRDKFFWLSDEEFARETLAGANPNSIQLVKAIIEWPLRSKLDAQIYGPPESAITREVIESQIIGYTTVEEAIQEKKLFVLDYHDLYLPYVSKVREMKNTTLYGSRTLFFLTKQGTLKPIVIELTRPIIGDKPQWKQIFTPASDSTNLWLWRLAKAHVLTHDTGYHELISHWLRTHCVVEPFVIATNRQLSTMHPIYKLLHPHLRYTLQINALGREILISSYGVIESTFFTKKYSMELSSVAYDKLWQFDLQGLPNDLLHRGMAVEDPNAQHGLKLAIEDYPFANDGLLIWDAIKQWVTDYVNHYYPNPSIIESDQELQAWWAEVRTKGHGDKSEEPWWPNLKTQKDLIDIITTIAWVASAHHSATNFAQYAYGGYFPNRPTIARNNMPTEDPTKEEWEKFLNKPEQTLLECFPSQIQATLVMTILNLLSYHSPDEEYLGQLIEPSWDENPDIKVAFERFNGRLKEIEGIIDSRNANSNLKNRNGAGIVPYELMKPFSGPGVTGKGVPYSISI*