AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0517350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517350 MtrunA17_Chr3g0118651 94.592 906 44 2 1 904 1 903 0.0 1758
Msa0517350 MtrunA17_Chr3g0118641 76.652 908 202 6 1 904 1 902 0.0 1451
Msa0517350 MtrunA17_Chr3g0118621 74.588 850 199 7 67 904 21 865 0.0 1276
Msa0517350 MtrunA17_Chr4g0033171 64.598 709 242 5 198 904 24 725 0.0 990
Msa0517350 MtrunA17_Chr8g0344201 47.636 846 415 12 76 903 91 926 0.0 811
Msa0517350 MtrunA17_Chr8g0377101 47.170 848 418 14 76 904 80 916 0.0 801
Msa0517350 MtrunA17_Chr1g0191921 43.105 863 462 13 62 904 5 858 0.0 696
Msa0517350 MtrunA17_Chr1g0205611 45.616 787 397 13 136 904 83 856 0.0 687
Msa0517350 MtrunA17_Chr4g0047281 42.081 884 454 18 42 903 47 894 0.0 678
Msa0517350 MtrunA17_Chr8g0342881 43.504 839 433 17 86 904 39 856 0.0 672
Msa0517350 MtrunA17_Chr8g0342971 43.073 859 435 18 80 904 32 870 0.0 671
Msa0517350 MtrunA17_Chr8g0343031 42.702 829 439 15 92 904 57 865 0.0 670
Msa0517350 MtrunA17_Chr2g0329911 43.217 833 440 16 87 904 47 861 0.0 669
Msa0517350 MtrunA17_Chr8g0343021 43.079 838 438 15 88 904 45 864 0.0 669
Msa0517350 MtrunA17_Chr8g0342921 42.972 868 443 17 60 904 31 869 0.0 664
Msa0517350 MtrunA17_Chr2g0329901 42.909 832 441 15 89 904 50 863 0.0 659
Msa0517350 MtrunA17_Chr7g0272791 43.185 829 437 16 97 904 52 867 0.0 658
Msa0517350 MtrunA17_Chr5g0406871 41.514 872 465 17 56 904 18 867 0.0 657
Msa0517350 MtrunA17_Chr8g0343011 44.228 771 409 11 139 904 111 865 0.0 655
Msa0517350 MtrunA17_Chr8g0342891 45.174 777 398 13 139 904 102 861 0.0 650
Msa0517350 MtrunA17_Chr8g0342931 45.241 767 392 14 149 904 1 750 0.0 648
Msa0517350 MtrunA17_Chr8g0344211 43.606 782 413 12 134 904 104 868 0.0 645
Msa0517350 MtrunA17_Chr8g0342901 41.935 837 437 16 80 904 29 828 0.0 639
Msa0517350 MtrunA17_Chr8g0342951 44.114 773 404 12 143 904 113 868 0.0 627
Msa0517350 MtrunA17_Chr3g0120071 40.860 837 458 16 88 904 39 858 0.0 619
Msa0517350 MtrunA17_Chr8g0342981 43.473 766 385 17 153 904 92 823 0.0 594
Msa0517350 MtrunA17_Chr8g0342991 42.670 764 379 11 146 904 98 807 0.0 570
Msa0517350 MtrunA17_Chr8g0343001 42.016 764 384 11 146 904 98 807 0.0 555
Msa0517350 MtrunA17_Chr8g0338451 38.758 805 446 19 110 887 66 850 0.0 550
Msa0517350 MtrunA17_Chr8g0344241 45.025 402 207 5 510 904 67 461 1.52e-112 353
Msa0517350 MtrunA17_Chr6g0471951 37.251 502 262 10 366 859 18 474 3.15e-105 335
Msa0517350 MtrunA17_Chr7g0218231 97.479 119 3 0 554 672 14 132 1.66e-76 246
Msa0517350 MtrunA17_Chr6g0468081 97.753 89 2 0 584 672 1 89 4.88e-54 182
Msa0517350 MtrunA17_Chr4g0033181 47.531 162 75 3 61 213 60 220 6.27e-40 148
Msa0517350 MtrunA17_Chr4g0047271 46.154 91 48 1 814 903 1 91 6.52e-18 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517350 AT3G45140.1 52.839 863 373 10 60 904 50 896 0.0 937
Msa0517350 AT3G45140.2 56.732 765 315 7 145 904 1 754 0.0 915
Msa0517350 AT1G72520.1 47.585 849 411 17 76 904 92 926 0.0 798
Msa0517350 AT1G17420.1 45.909 880 444 13 45 904 52 919 0.0 796
Msa0517350 AT1G67560.1 46.806 814 410 13 103 904 115 917 0.0 756
Msa0517350 AT1G55020.1 45.300 883 436 17 41 904 5 859 0.0 715
Msa0517350 AT3G22400.1 43.818 833 427 15 101 904 66 886 0.0 682

Find 189 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAGCAATGTTAAATTAT+TGG 0.149707 3_4:-68946528 None:intergenic
ATTCTGGTGGACCATAAATT+TGG 0.173285 3_4:-68946973 None:intergenic
CTAGCAATTGTAGGTCTATT+AGG 0.189376 3_4:-68948259 None:intergenic
AAATTGGATGCCCAAATTTA+TGG 0.209486 3_4:+68946962 Msa0517350:CDS
CAATTATTGCCTTGACTAAT+TGG 0.209625 3_4:-68946827 None:intergenic
TTTGAGTTACCAGCAGATTT+TGG 0.210007 3_4:+68945472 Msa0517350:CDS
TTGACTAATTGGATGCTATT+TGG 0.210653 3_4:-68946816 None:intergenic
CTTCTGATTCTACTAATCTT+TGG 0.241953 3_4:+68947348 Msa0517350:CDS
TGACTAATTGGATGCTATTT+GGG 0.253957 3_4:-68946815 None:intergenic
GAGCTCACTAGACCGATTAT+TGG 0.258181 3_4:+68947290 Msa0517350:CDS
ATAGCATTTGAAAGGTTTAA+TGG 0.262850 3_4:+68948490 Msa0517350:CDS
TCAAATGCTATCTTTATATC+TGG 0.267837 3_4:-68948478 None:intergenic
GTTGCTACAACAAAAGTAAT+TGG 0.272082 3_4:+68944885 Msa0517350:CDS
AACTTTGCACAATATGCTTA+TGG 0.278802 3_4:+68948226 Msa0517350:CDS
CAAACCTGAGCAAACACTTT+TGG 0.281831 3_4:+68948336 Msa0517350:CDS
CTAAATAACATAGTCCTTGA+TGG 0.285243 3_4:+68945544 Msa0517350:CDS
ATGGCCTCTGAGAAGTAAAT+TGG 0.305851 3_4:+68946946 Msa0517350:CDS
TTTAGGCCATGTTGTGCATT+AGG 0.307604 3_4:-68947929 None:intergenic
GTGAAGAAAACTCTCTTTGT+AGG 0.309146 3_4:-68945628 None:intergenic
CAAATGATGGTCTTCTCATA+TGG 0.312417 3_4:+68947978 Msa0517350:CDS
GATTCAATGATGCTTGGATT+TGG 0.313188 3_4:-68948046 None:intergenic
TAGCAATTGTAGGTCTATTA+GGG 0.314837 3_4:-68948258 None:intergenic
ATTCTACTAATCTTTGGCTT+TGG 0.315515 3_4:+68947354 Msa0517350:CDS
CATCTTCACCTTCCTATATA+AGG 0.323869 3_4:+68944846 Msa0517350:CDS
TATCAAAGCGCAAAAGGTTT+TGG 0.327729 3_4:-68946589 None:intergenic
TGGAAATGTGGGCGCTGTTT+TGG 0.329432 3_4:+68945492 Msa0517350:CDS
CCTTTGTGATTGCAACAAAT+AGG 0.332818 3_4:+68947551 Msa0517350:CDS
CTTTGTAGGTGTATCATGCT+TGG 0.333114 3_4:-68945614 None:intergenic
GTGACTCCAGGTCCTGAAAA+TGG 0.335649 3_4:-68948613 None:intergenic
TAGTACTAGTACAAGTAAAA+AGG 0.340965 3_4:+68944934 Msa0517350:CDS
CTACTAATCTTTGGCTTTGG+AGG 0.343876 3_4:+68947357 Msa0517350:CDS
AAACTCAAAAGACCTGTTCT+TGG 0.345034 3_4:+68945966 Msa0517350:CDS
GCTTTGGCAATTTGATTTGC+AGG 0.346703 3_4:+68947744 Msa0517350:CDS
TCAACAAAAGCGTCATAGTT+TGG 0.349961 3_4:-68944784 None:intergenic
GTCATCGAGTCGCAGATAAT+TGG 0.351690 3_4:+68947013 Msa0517350:CDS
TACTAAATAGATATGCTATA+TGG 0.354697 3_4:-68948649 None:intergenic
TTCTCCCATTCTTCCTTTGT+AGG 0.357198 3_4:-68948304 None:intergenic
CTCATCAAGAATATTGTTAC+TGG 0.359156 3_4:+68945055 Msa0517350:CDS
AATGGTTTCATTAGCTCATA+AGG 0.360058 3_4:-68948595 None:intergenic
TGGCTTCAATGTGCCTTGTT+TGG 0.360768 3_4:-68947258 None:intergenic
TTGCAGGAGACAAATTCTTT+TGG 0.363534 3_4:+68946748 Msa0517350:intron
TAGCATTTGAAAGGTTTAAT+GGG 0.363606 3_4:+68948491 Msa0517350:CDS
TAACCAACTATAAGATATCT+TGG 0.364041 3_4:+68944749 Msa0517350:five_prime_UTR
TCCCTCACTTTGCTTACATA+TGG 0.365328 3_4:-68947188 None:intergenic
CAAATACTAATTTGAAGAAT+AGG 0.370666 3_4:+68948554 Msa0517350:CDS
AATATTGTTACTGGTATTGT+TGG 0.378392 3_4:+68945064 Msa0517350:CDS
AGTCTTCTATTGCTAGCTTT+AGG 0.378934 3_4:-68947946 None:intergenic
ATTCTCACAGACATAAATCT+TGG 0.383754 3_4:+68946437 Msa0517350:CDS
AAACAATTTCTTCTCTTGAA+TGG 0.386249 3_4:-68947138 None:intergenic
TCTCTCGTGATAGCTGATTC+TGG 0.393236 3_4:-68946989 None:intergenic
GAGATTCTTATTAGTTCATA+TGG 0.393969 3_4:+68947655 Msa0517350:CDS
CCTTGGATACGGATATTGTT+TGG 0.398283 3_4:-68945994 None:intergenic
TTTGTAGGTGTATCATGCTT+GGG 0.400836 3_4:-68945613 None:intergenic
CTGTGGCATATGATAAGCTT+TGG 0.401553 3_4:+68947728 Msa0517350:CDS
ATGTTGTTTCTAGCAATTGT+AGG 0.408062 3_4:-68948268 None:intergenic
TCCATCTTAGTTGTCGATCA+TGG 0.412724 3_4:+68945584 Msa0517350:CDS
TTGAGTTTAATGTTGGTCTC+AGG 0.412871 3_4:-68945951 None:intergenic
TTTGCTAGGGAGACTTTGGC+AGG 0.413977 3_4:+68946786 Msa0517350:CDS
TATGATGTCTACAATGATCT+TGG 0.416200 3_4:+68945924 Msa0517350:CDS
TTTCAATTACCATCATCTCT+TGG 0.418695 3_4:+68946503 Msa0517350:CDS
TGTGGTTTGTCGCCAATAAT+CGG 0.420168 3_4:-68947302 None:intergenic
TTCTGGTGGACCATAAATTT+GGG 0.424260 3_4:-68946972 None:intergenic
TGAGGCGTTTGCTGAGATAA+AGG 0.424456 3_4:+68946349 Msa0517350:CDS
TGATCAGATTCAATGATGCT+TGG 0.424472 3_4:-68948052 None:intergenic
ACCAGTGGCTTATAAGTTCA+TGG 0.425133 3_4:-68947418 None:intergenic
TCTCCCATTCTTCCTTTGTA+GGG 0.428559 3_4:-68948303 None:intergenic
ATCCCAAGATATCTTATAGT+TGG 0.429910 3_4:-68944752 None:intergenic
CTAAGCTTTCTTAATCCACT+TGG 0.430925 3_4:-68945831 None:intergenic
ATGAAGAATACTACACTGTA+TGG 0.431751 3_4:+68947212 Msa0517350:CDS
CACTCCAAAAGTGTTTGCTC+AGG 0.438001 3_4:-68948340 None:intergenic
TTCACCAGATGAAGAGTACT+TGG 0.441500 3_4:+68948423 Msa0517350:CDS
CATGTTCTTACTCATGATAC+TGG 0.449029 3_4:+68947392 Msa0517350:CDS
AGAAGACCATCATTTGCAAA+AGG 0.449584 3_4:-68947971 None:intergenic
GTATGATAATAAGTTCAAAA+TGG 0.450817 3_4:-68948398 None:intergenic
CTAGTGAGCTCAATGACTAT+TGG 0.450930 3_4:-68947278 None:intergenic
GGTCGAAAGCATTCTAATAC+AGG 0.461714 3_4:+68946029 Msa0517350:CDS
TACCGGTGGAGAAGATCATT+TGG 0.465498 3_4:-68947772 None:intergenic
AAGGTTTAATGGGAGATTGA+AGG 0.467037 3_4:+68948501 Msa0517350:CDS
GGAATTTGCTAGGGAGACTT+TGG 0.467432 3_4:+68946782 Msa0517350:CDS
TGTTTGGTGCCACCAAGAAC+AGG 0.467750 3_4:-68945978 None:intergenic
ACTCCCTTTCCAGTGACTCC+AGG 0.469105 3_4:-68948625 None:intergenic
TGTCCCAAGTACTCTTCATC+TGG 0.470767 3_4:-68948427 None:intergenic
ATTGTGGAAATGTGATATAT+AGG 0.473415 3_4:-68948723 None:intergenic
ATGGGATGCAATTAAACAAT+GGG 0.473897 3_4:+68947997 Msa0517350:CDS
AAGACCCTACAAAGGAAGAA+TGG 0.474435 3_4:+68948299 Msa0517350:CDS
CTAATAGAACTAAGAGGGAA+TGG 0.477908 3_4:+68945864 Msa0517350:CDS
GATGAGCTAATAGAACTAAG+AGG 0.478619 3_4:+68945858 Msa0517350:CDS
CTAAGATGGACAGGTCCATC+AGG 0.482847 3_4:-68945571 None:intergenic
TCCTTTGTAGGGTCTTCTGT+AGG 0.484655 3_4:-68948292 None:intergenic
GACTTTAATCAACCTGGGAA+TGG 0.485837 3_4:-68946555 None:intergenic
CTATTTGTTGCAATCACAAA+GGG 0.485842 3_4:-68947550 None:intergenic
AGACCCTACAAAGGAAGAAT+GGG 0.490464 3_4:+68948300 Msa0517350:CDS
TTGAAGAATAGGAATGGAGC+TGG 0.490528 3_4:+68948565 Msa0517350:CDS
ATAGACGAACTTTACAAAGA+AGG 0.490922 3_4:+68946479 Msa0517350:CDS
TAATTGGATGCTATTTGGGT+TGG 0.495555 3_4:-68946811 None:intergenic
GGACCTGGAGTCACTGGAAA+GGG 0.496747 3_4:+68948622 Msa0517350:CDS
CAGGGGTATCAAAGCGCAAA+AGG 0.499404 3_4:-68946595 None:intergenic
TATCCAAGGCGTTGTCGTAC+CGG 0.500621 3_4:+68946008 Msa0517350:CDS
AGAGAATGAGGTGCAATATG+AGG 0.502465 3_4:+68945444 Msa0517350:CDS
CATAATCACCACTATAGCTT+GGG 0.503457 3_4:+68948177 Msa0517350:CDS
TGGACAGAAGTTCGAACAAA+AGG 0.507956 3_4:+68948091 Msa0517350:CDS
CGACCGGTACGACAACGCCT+TGG 0.509007 3_4:-68946011 None:intergenic
GACAACTCATTGAACCATCA+TGG 0.509837 3_4:+68948446 Msa0517350:CDS
ACATAATCACCACTATAGCT+TGG 0.510325 3_4:+68948176 Msa0517350:CDS
AACACAACCACGGCGTCCTC+AGG 0.513052 3_4:+68946383 Msa0517350:CDS
ATCATACGAAAAGAGAGATT+CGG 0.513208 3_4:+68946307 Msa0517350:CDS
AACCAACTATAAGATATCTT+GGG 0.519149 3_4:+68944750 Msa0517350:five_prime_UTR
AGTACTCTTCATCTGGTGAA+TGG 0.520631 3_4:-68948420 None:intergenic
CCCACATTTCCAAAATCTGC+TGG 0.525769 3_4:-68945481 None:intergenic
TTTGTTGCCATCACAATGCA+AGG 0.526504 3_4:-68944965 None:intergenic
TCCATGATCGACAACTAAGA+TGG 0.527836 3_4:-68945585 None:intergenic
GTGAATATTTGTTTCCATTG+TGG 0.529219 3_4:-68947320 None:intergenic
ACTAATTTGAAGAATAGGAA+TGG 0.529661 3_4:+68948559 Msa0517350:CDS
CTCAAAAGACCTGTTCTTGG+TGG 0.530119 3_4:+68945969 Msa0517350:CDS
GAACTAAGAGGGAATGGAGA+AGG 0.533603 3_4:+68945870 Msa0517350:CDS
AACAACAACAAAAGTAATTG+TGG 0.533894 3_4:-68948739 None:intergenic
TTCGCAAATTCAAGCAACCT+TGG 0.534313 3_4:+68948369 Msa0517350:CDS
GTACGACAACGCCTTGGATA+CGG 0.536846 3_4:-68946005 None:intergenic
GGGAGATTGAAGGAAATTGA+AGG 0.543222 3_4:+68948511 Msa0517350:CDS
AGATGCTACCCAAGCTATAG+TGG 0.544008 3_4:-68948185 None:intergenic
CATATGGTAAATATAAATCA+TGG 0.544415 3_4:-68947172 None:intergenic
GGATGCAGTAACTTGTAGAT+TGG 0.544887 3_4:-68947592 None:intergenic
GAAGACCATCATTTGCAAAA+GGG 0.545653 3_4:-68947970 None:intergenic
ATATAAAGATAGCATTTGAA+AGG 0.547239 3_4:+68948482 Msa0517350:CDS
TTGTTCTTCCTCACCAAACA+AGG 0.547255 3_4:+68947245 Msa0517350:CDS
AATCCACTTGGTGTTTGTGA+TGG 0.549751 3_4:-68945819 None:intergenic
ATTAGCGACTTTAATCAACC+TGG 0.555673 3_4:-68946561 None:intergenic
TATCTGATGAGGAATTTGCT+AGG 0.559728 3_4:+68946772 Msa0517350:CDS
TGAAGAATAGGAATGGAGCT+GGG 0.560809 3_4:+68948566 Msa0517350:CDS
TGCTAGGGAGACTTTGGCAG+GGG 0.560834 3_4:+68946788 Msa0517350:CDS
CTTTGGCAATTTGATTTGCA+GGG 0.562249 3_4:+68947745 Msa0517350:CDS
TTATTGGATCCAAGAGATGA+TGG 0.563781 3_4:-68946512 None:intergenic
TGGTTATACCACAGTTGAAG+AGG 0.565567 3_4:+68947033 Msa0517350:CDS
TGTTTATCCTTGCATTGTGA+TGG 0.568663 3_4:+68944958 Msa0517350:CDS
ATCTGATGAGGAATTTGCTA+GGG 0.573680 3_4:+68946773 Msa0517350:CDS
GATCGACAACTAAGATGGAC+AGG 0.574532 3_4:-68945580 None:intergenic
ACCATGAACTTATAAGCCAC+TGG 0.574627 3_4:+68947417 Msa0517350:CDS
TCTGGATTCTCATCCCATGA+TGG 0.575349 3_4:-68948460 None:intergenic
ACAACTCATTGAACCATCAT+GGG 0.576187 3_4:+68948447 Msa0517350:CDS
AGATTAGTAGAATCAGAAGC+AGG 0.576251 3_4:-68947344 None:intergenic
CCTATTTGTTGCAATCACAA+AGG 0.577748 3_4:-68947551 None:intergenic
TATGGGATGCAATTAAACAA+TGG 0.577797 3_4:+68947996 Msa0517350:CDS
GATGCATTGTTAATGATGAA+TGG 0.578040 3_4:-68944827 None:intergenic
AGGACCTGGAGTCACTGGAA+AGG 0.579892 3_4:+68948621 Msa0517350:CDS
CCAAACAATATCCGTATCCA+AGG 0.581077 3_4:+68945994 Msa0517350:CDS
TGTATTAGAATGCTTTCGAC+CGG 0.581752 3_4:-68946027 None:intergenic
ATGAGCTAATAGAACTAAGA+GGG 0.582546 3_4:+68945859 Msa0517350:CDS
ACCATATGTAAGCAAAGTGA+GGG 0.586108 3_4:+68947187 Msa0517350:CDS
TCCAAGTGCATTTATCTGCA+AGG 0.586289 3_4:-68947629 None:intergenic
AAATGATGGTCTTCTCATAT+GGG 0.587188 3_4:+68947979 Msa0517350:CDS
AGGTTGCTTGAATTTGCGAA+GGG 0.588741 3_4:-68948366 None:intergenic
ACCTTGCAGATAAATGCACT+TGG 0.589225 3_4:+68947628 Msa0517350:CDS
CACATTATGAGATTTAGACT+CGG 0.593059 3_4:-68944996 None:intergenic
TTTCTATGTTCCTCGAGATG+AGG 0.593473 3_4:+68946331 Msa0517350:CDS
TTCAATGGAATTGAGTTCTG+TGG 0.597142 3_4:+68947711 Msa0517350:CDS
AAGGTTGCTTGAATTTGCGA+AGG 0.599155 3_4:-68948367 None:intergenic
GTCCATCAGGAAAACCATCA+AGG 0.601868 3_4:-68945558 None:intergenic
TACCATATGTAAGCAAAGTG+AGG 0.603607 3_4:+68947186 Msa0517350:CDS
TACCAAATGATCTTCTCCAC+CGG 0.604794 3_4:+68947770 Msa0517350:CDS
TTACCATCACAAACACCAAG+TGG 0.605695 3_4:+68945816 Msa0517350:CDS
TCAGCAAACGCCTCATCTCG+AGG 0.612448 3_4:-68946341 None:intergenic
TCAATGTGCCTTGTTTGGTG+AGG 0.613172 3_4:-68947253 None:intergenic
GTCTGTGAGAATTGTGTCCA+AGG 0.613851 3_4:-68946426 None:intergenic
GCTTAGTACCTCTTCAACTG+TGG 0.616160 3_4:-68947041 None:intergenic
CTGATCAAGAGCTTCAAGCA+TGG 0.620687 3_4:+68948068 Msa0517350:CDS
TTAGCGACTTTAATCAACCT+GGG 0.622026 3_4:-68946560 None:intergenic
TAACTTGTAGATTGGATGCA+TGG 0.623713 3_4:-68947584 None:intergenic
TTTGCACAATATGCTTATGG+AGG 0.625959 3_4:+68948229 Msa0517350:CDS
AAATAGCATCCAATTAGTCA+AGG 0.636861 3_4:+68946818 Msa0517350:CDS
ATCAAGAGCTTCAAGCATGG+TGG 0.642027 3_4:+68948071 Msa0517350:CDS
TGCAAGGTATATCTGAGATG+TGG 0.649204 3_4:-68947613 None:intergenic
GAAGTTCGAACAAAAGGTCA+CGG 0.654694 3_4:+68948097 Msa0517350:CDS
CTCGTGATAGCTGATTCTGG+TGG 0.654841 3_4:-68946986 None:intergenic
GCATCCAATTTACTTCTCAG+AGG 0.654968 3_4:-68946950 None:intergenic
GTTCAAAATGGTCATCACCA+AGG 0.657548 3_4:-68948386 None:intergenic
TTGCTAGGGAGACTTTGGCA+GGG 0.658261 3_4:+68946787 Msa0517350:CDS
TAATGTGAAAGCTTTAGTGA+CGG 0.659359 3_4:+68945012 Msa0517350:CDS
TAGTGGTGATTATGTCAATG+AGG 0.663602 3_4:-68948168 None:intergenic
TCACCAGATGAAGAGTACTT+GGG 0.668317 3_4:+68948424 Msa0517350:CDS
TGAAAGCTTTAGTGACGGTG+AGG 0.677954 3_4:+68945017 Msa0517350:CDS
TTATTGGCGACAAACCACAA+TGG 0.686808 3_4:+68947306 Msa0517350:CDS
ATGAATGGTCGATGAACATG+TGG 0.687524 3_4:-68944812 None:intergenic
GAAGATCCTAATGCACAACA+TGG 0.692109 3_4:+68947923 Msa0517350:CDS
GCGACAAATCAGAAGAACCA+TGG 0.695121 3_4:+68948119 Msa0517350:CDS
GTGACGGTGAGGCAAAGTGA+TGG 0.699606 3_4:+68945028 Msa0517350:CDS
GCCTACAGAAGACCCTACAA+AGG 0.701209 3_4:+68948291 Msa0517350:CDS
ACGGTGAGGCAAAGTGATGG+TGG 0.702005 3_4:+68945031 Msa0517350:CDS
GATACAATTCAACACAACCA+CGG 0.729752 3_4:+68946373 Msa0517350:CDS
ACAAATCAGAAGAACCATGG+TGG 0.737932 3_4:+68948122 Msa0517350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAAGTTTTATATATACA+TGG + chr3_4:68945705-68945724 Msa0517350:intron 10.0%
!! CAAGTAATATAATAAATATT+TGG - chr3_4:68946695-68946714 None:intergenic 10.0%
!! ATTAAAACATAAATAAACCA+TGG - chr3_4:68945206-68945225 None:intergenic 15.0%
!! TCAAAATTATTTATATATGC+TGG - chr3_4:68946091-68946110 None:intergenic 15.0%
!! CAATAATTGTTACAAATATT+TGG + chr3_4:68946841-68946860 Msa0517350:intron 15.0%
!!! TATATGTTGTTGATTTTTAT+AGG + chr3_4:68947503-68947522 Msa0517350:intron 15.0%
!!! AGAATATTTTCTCCTTATAT+AGG - chr3_4:68944861-68944880 None:intergenic 20.0%
!! AATTTACTAGTAACAATGAA+TGG - chr3_4:68945280-68945299 None:intergenic 20.0%
!! ATTTGAATAAAATAAACGTG+AGG - chr3_4:68945365-68945384 None:intergenic 20.0%
!!! CGTTTATTTTATTCAAATGA+AGG + chr3_4:68945367-68945386 Msa0517350:intron 20.0%
!! AATAGCATACAAATTAAACT+TGG - chr3_4:68945762-68945781 None:intergenic 20.0%
!! ATATGTTGTAAATTAGAGAA+AGG - chr3_4:68946133-68946152 None:intergenic 20.0%
!!! CATGAGAATACTTTTTTATT+TGG - chr3_4:68946165-68946184 None:intergenic 20.0%
!!! TTTTACAAGTTATTTGAGTT+GGG + chr3_4:68946715-68946734 Msa0517350:intron 20.0%
!! TTATATCATCTATGCTAATT+AGG + chr3_4:68946878-68946897 Msa0517350:intron 20.0%
!!! GAAATCTTTTGTTTTTTTCT+TGG + chr3_4:68946900-68946919 Msa0517350:intron 20.0%
!! GTGTATTAATTATGTTATGT+AGG + chr3_4:68946922-68946941 Msa0517350:intron 20.0%
!! TTAATTATGTTATGTAGGAA+TGG + chr3_4:68946927-68946946 Msa0517350:intron 20.0%
!! CATATGGTAAATATAAATCA+TGG - chr3_4:68947175-68947194 None:intergenic 20.0%
!!! TTGTTGATTTTTATAGGTTA+AGG + chr3_4:68947509-68947528 Msa0517350:intron 20.0%
!!! CTTTACTAAAAAGTATTCAA+TGG + chr3_4:68947696-68947715 Msa0517350:CDS 20.0%
!! GTATGATAATAAGTTCAAAA+TGG - chr3_4:68948401-68948420 None:intergenic 20.0%
!! ATATAAAGATAGCATTTGAA+AGG + chr3_4:68948482-68948501 Msa0517350:CDS 20.0%
!! CAAATACTAATTTGAAGAAT+AGG + chr3_4:68948554-68948573 Msa0517350:CDS 20.0%
!! TACTAAATAGATATGCTATA+TGG - chr3_4:68948652-68948671 None:intergenic 20.0%
! TAACCAACTATAAGATATCT+TGG + chr3_4:68944749-68944768 Msa0517350:five_prime_UTR 25.0%
! AACCAACTATAAGATATCTT+GGG + chr3_4:68944750-68944769 Msa0517350:five_prime_UTR 25.0%
!! TATTTTCTCCTTATATAGGA+AGG - chr3_4:68944857-68944876 None:intergenic 25.0%
! TAGTACTAGTACAAGTAAAA+AGG + chr3_4:68944934-68944953 Msa0517350:CDS 25.0%
! AATATTGTTACTGGTATTGT+TGG + chr3_4:68945064-68945083 Msa0517350:CDS 25.0%
! GCAATAAAATGAAACTTACT+TGG - chr3_4:68945133-68945152 None:intergenic 25.0%
! AACATAAATAAACCATGGTT+TGG - chr3_4:68945201-68945220 None:intergenic 25.0%
! TATGTATGTACACAAAACTT+TGG + chr3_4:68945250-68945269 Msa0517350:intron 25.0%
! TCAGAGAAAAAGACAATAAA+AGG + chr3_4:68945397-68945416 Msa0517350:CDS 25.0%
!!! AAAAACAATTTTGGAATCCT+TGG + chr3_4:68946409-68946428 Msa0517350:CDS 25.0%
! TTGGAGCAATGTTAAATTAT+TGG - chr3_4:68946531-68946550 None:intergenic 25.0%
!! GTTTTACAAGTTATTTGAGT+TGG + chr3_4:68946714-68946733 Msa0517350:intron 25.0%
!!! TGTTTAATTTGAGTATATGC+AGG + chr3_4:68947115-68947134 Msa0517350:intron 25.0%
! AAACAATTTCTTCTCTTGAA+TGG - chr3_4:68947141-68947160 None:intergenic 25.0%
!! ATCAATTTTTACCTTGATTC+CGG + chr3_4:68947453-68947472 Msa0517350:intron 25.0%
! GAGATTCTTATTAGTTCATA+TGG + chr3_4:68947655-68947674 Msa0517350:CDS 25.0%
! GATATAGTGAGAGAAATTTA+GGG - chr3_4:68947858-68947877 None:intergenic 25.0%
! TGATATAGTGAGAGAAATTT+AGG - chr3_4:68947859-68947878 None:intergenic 25.0%
!! TCTTTTTGTGTTTTCAGATT+TGG - chr3_4:68948148-68948167 None:intergenic 25.0%
! TCAAATGCTATCTTTATATC+TGG - chr3_4:68948481-68948500 None:intergenic 25.0%
! ATAGCATTTGAAAGGTTTAA+TGG + chr3_4:68948490-68948509 Msa0517350:CDS 25.0%
! TAGCATTTGAAAGGTTTAAT+GGG + chr3_4:68948491-68948510 Msa0517350:CDS 25.0%
! ACTAATTTGAAGAATAGGAA+TGG + chr3_4:68948559-68948578 Msa0517350:CDS 25.0%
! ATTGTGGAAATGTGATATAT+AGG - chr3_4:68948726-68948745 None:intergenic 25.0%
! AACAACAACAAAAGTAATTG+TGG - chr3_4:68948742-68948761 None:intergenic 25.0%
!! TTTTTCTTGAATCTCTTTGA+TGG + chr3_4:68948791-68948810 Msa0517350:three_prime_UTR 25.0%
ATCCCAAGATATCTTATAGT+TGG - chr3_4:68944755-68944774 None:intergenic 30.0%
GATGCATTGTTAATGATGAA+TGG - chr3_4:68944830-68944849 None:intergenic 30.0%
GTTGCTACAACAAAAGTAAT+TGG + chr3_4:68944885-68944904 Msa0517350:CDS 30.0%
CACATTATGAGATTTAGACT+CGG - chr3_4:68944999-68945018 None:intergenic 30.0%
! TAATGTGAAAGCTTTAGTGA+CGG + chr3_4:68945012-68945031 Msa0517350:CDS 30.0%
CTCATCAAGAATATTGTTAC+TGG + chr3_4:68945055-68945074 Msa0517350:CDS 30.0%
!! TGGTAACAAACACTTGTTTT+TGG + chr3_4:68945084-68945103 Msa0517350:CDS 30.0%
ATATGAACACAATTCTTCGA+AGG + chr3_4:68945330-68945349 Msa0517350:intron 30.0%
ATAAAATAAACGTGAGGTCT+AGG - chr3_4:68945359-68945378 None:intergenic 30.0%
AACTGAGAAAAAAGAGAATG+AGG + chr3_4:68945432-68945451 Msa0517350:CDS 30.0%
CTAAATAACATAGTCCTTGA+TGG + chr3_4:68945544-68945563 Msa0517350:CDS 30.0%
CTTGGTCATGTAAATAAAGT+TGG - chr3_4:68945744-68945763 None:intergenic 30.0%
ATGAGCTAATAGAACTAAGA+GGG + chr3_4:68945859-68945878 Msa0517350:CDS 30.0%
TATGATGTCTACAATGATCT+TGG + chr3_4:68945924-68945943 Msa0517350:CDS 30.0%
!!! AGGTCTTTTGAGTTTAATGT+TGG - chr3_4:68945961-68945980 None:intergenic 30.0%
CTGGACAACTAAAATGAAAT+AGG - chr3_4:68946072-68946091 None:intergenic 30.0%
ATCATACGAAAAGAGAGATT+CGG + chr3_4:68946307-68946326 Msa0517350:CDS 30.0%
!!! CAAAATTGTTTTTACTCCTG+AGG - chr3_4:68946402-68946421 None:intergenic 30.0%
! CTCAGGAGTAAAAACAATTT+TGG + chr3_4:68946400-68946419 Msa0517350:CDS 30.0%
ATTCTCACAGACATAAATCT+TGG + chr3_4:68946437-68946456 Msa0517350:CDS 30.0%
ATAGACGAACTTTACAAAGA+AGG + chr3_4:68946479-68946498 Msa0517350:CDS 30.0%
TTTCAATTACCATCATCTCT+TGG + chr3_4:68946503-68946522 Msa0517350:CDS 30.0%
! TGACTAATTGGATGCTATTT+GGG - chr3_4:68946818-68946837 None:intergenic 30.0%
TTGACTAATTGGATGCTATT+TGG - chr3_4:68946819-68946838 None:intergenic 30.0%
AAATAGCATCCAATTAGTCA+AGG + chr3_4:68946818-68946837 Msa0517350:CDS 30.0%
CAATTATTGCCTTGACTAAT+TGG - chr3_4:68946830-68946849 None:intergenic 30.0%
AAATTGGATGCCCAAATTTA+TGG + chr3_4:68946962-68946981 Msa0517350:CDS 30.0%
ATGAAGAATACTACACTGTA+TGG + chr3_4:68947212-68947231 Msa0517350:CDS 30.0%
GTGAATATTTGTTTCCATTG+TGG - chr3_4:68947323-68947342 None:intergenic 30.0%
CTTCTGATTCTACTAATCTT+TGG + chr3_4:68947348-68947367 Msa0517350:CDS 30.0%
! ATTCTACTAATCTTTGGCTT+TGG + chr3_4:68947354-68947373 Msa0517350:CDS 30.0%
! CTATTTGTTGCAATCACAAA+GGG - chr3_4:68947553-68947572 None:intergenic 30.0%
ATGATACGTATAGACTTTAC+CGG - chr3_4:68947792-68947811 None:intergenic 30.0%
!! GCAAATTGTTGTTCTTTTAG+AGG + chr3_4:68947890-68947909 Msa0517350:intron 30.0%
!! TTGTTGTTCTTTTAGAGGAA+TGG + chr3_4:68947895-68947914 Msa0517350:intron 30.0%
AAATGATGGTCTTCTCATAT+GGG + chr3_4:68947979-68947998 Msa0517350:CDS 30.0%
TATGGGATGCAATTAAACAA+TGG + chr3_4:68947996-68948015 Msa0517350:CDS 30.0%
ATGGGATGCAATTAAACAAT+GGG + chr3_4:68947997-68948016 Msa0517350:CDS 30.0%
AACTTTGCACAATATGCTTA+TGG + chr3_4:68948226-68948245 Msa0517350:CDS 30.0%
TAGCAATTGTAGGTCTATTA+GGG - chr3_4:68948261-68948280 None:intergenic 30.0%
!! ATGTTGTTTCTAGCAATTGT+AGG - chr3_4:68948271-68948290 None:intergenic 30.0%
AATGGTTTCATTAGCTCATA+AGG - chr3_4:68948598-68948617 None:intergenic 30.0%
!! TCAACAAAAGCGTCATAGTT+TGG - chr3_4:68944787-68944806 None:intergenic 35.0%
CATCTTCACCTTCCTATATA+AGG + chr3_4:68944846-68944865 Msa0517350:CDS 35.0%
TGTTTATCCTTGCATTGTGA+TGG + chr3_4:68944958-68944977 Msa0517350:CDS 35.0%
GATGTTACTGTAAAGGACAA+TGG - chr3_4:68945179-68945198 None:intergenic 35.0%
! TGGTTTGGATGTTACTGTAA+AGG - chr3_4:68945186-68945205 None:intergenic 35.0%
TTACAGTAACATCCAAACCA+TGG + chr3_4:68945186-68945205 Msa0517350:intron 35.0%
!! TTTGAGTTACCAGCAGATTT+TGG + chr3_4:68945472-68945491 Msa0517350:CDS 35.0%
TTTGTAGGTGTATCATGCTT+GGG - chr3_4:68945616-68945635 None:intergenic 35.0%
GTGAAGAAAACTCTCTTTGT+AGG - chr3_4:68945631-68945650 None:intergenic 35.0%
! GAGAGTTTTCTTCACTAACA+AGG + chr3_4:68945636-68945655 Msa0517350:CDS 35.0%
CTAAGCTTTCTTAATCCACT+TGG - chr3_4:68945834-68945853 None:intergenic 35.0%
GATGAGCTAATAGAACTAAG+AGG + chr3_4:68945858-68945877 Msa0517350:CDS 35.0%
CTAATAGAACTAAGAGGGAA+TGG + chr3_4:68945864-68945883 Msa0517350:CDS 35.0%
! TTGAGTTTAATGTTGGTCTC+AGG - chr3_4:68945954-68945973 None:intergenic 35.0%
AAACTCAAAAGACCTGTTCT+TGG + chr3_4:68945966-68945985 Msa0517350:CDS 35.0%
TGTATTAGAATGCTTTCGAC+CGG - chr3_4:68946030-68946049 None:intergenic 35.0%
! GAATCTCTCTTTTCGTATGA+TGG - chr3_4:68946308-68946327 None:intergenic 35.0%
GATACAATTCAACACAACCA+CGG + chr3_4:68946373-68946392 Msa0517350:CDS 35.0%
TTATTGGATCCAAGAGATGA+TGG - chr3_4:68946515-68946534 None:intergenic 35.0%
TTAGCGACTTTAATCAACCT+GGG - chr3_4:68946563-68946582 None:intergenic 35.0%
ATTAGCGACTTTAATCAACC+TGG - chr3_4:68946564-68946583 None:intergenic 35.0%
! TATCAAAGCGCAAAAGGTTT+TGG - chr3_4:68946592-68946611 None:intergenic 35.0%
!! CTTACTTTTAAGTGCTTCAG+GGG - chr3_4:68946615-68946634 None:intergenic 35.0%
!! GCTTACTTTTAAGTGCTTCA+GGG - chr3_4:68946616-68946635 None:intergenic 35.0%
!! TGCTTACTTTTAAGTGCTTC+AGG - chr3_4:68946617-68946636 None:intergenic 35.0%
! TTGCAGGAGACAAATTCTTT+TGG + chr3_4:68946748-68946767 Msa0517350:intron 35.0%
!! ATTCTTTTGGCTATCTGATG+AGG + chr3_4:68946761-68946780 Msa0517350:CDS 35.0%
! TATCTGATGAGGAATTTGCT+AGG + chr3_4:68946772-68946791 Msa0517350:CDS 35.0%
!! ATCTGATGAGGAATTTGCTA+GGG + chr3_4:68946773-68946792 Msa0517350:CDS 35.0%
! TAATTGGATGCTATTTGGGT+TGG - chr3_4:68946814-68946833 None:intergenic 35.0%
! TTCTGGTGGACCATAAATTT+GGG - chr3_4:68946975-68946994 None:intergenic 35.0%
! ATTCTGGTGGACCATAAATT+TGG - chr3_4:68946976-68946995 None:intergenic 35.0%
TACCATATGTAAGCAAAGTG+AGG + chr3_4:68947186-68947205 Msa0517350:CDS 35.0%
ACCATATGTAAGCAAAGTGA+GGG + chr3_4:68947187-68947206 Msa0517350:CDS 35.0%
AGATTAGTAGAATCAGAAGC+AGG - chr3_4:68947347-68947366 None:intergenic 35.0%
CATGTTCTTACTCATGATAC+TGG + chr3_4:68947392-68947411 Msa0517350:CDS 35.0%
! CCTATTTGTTGCAATCACAA+AGG - chr3_4:68947554-68947573 None:intergenic 35.0%
CCTTTGTGATTGCAACAAAT+AGG + chr3_4:68947551-68947570 Msa0517350:CDS 35.0%
! TAACTTGTAGATTGGATGCA+TGG - chr3_4:68947587-68947606 None:intergenic 35.0%
TTCAATGGAATTGAGTTCTG+TGG + chr3_4:68947711-68947730 Msa0517350:CDS 35.0%
!! CTTTGGCAATTTGATTTGCA+GGG + chr3_4:68947745-68947764 Msa0517350:CDS 35.0%
ATAGTGAGAGAAATTTAGGG+AGG - chr3_4:68947855-68947874 None:intergenic 35.0%
!! AGTCTTCTATTGCTAGCTTT+AGG - chr3_4:68947949-68947968 None:intergenic 35.0%
GAAGACCATCATTTGCAAAA+GGG - chr3_4:68947973-68947992 None:intergenic 35.0%
AGAAGACCATCATTTGCAAA+AGG - chr3_4:68947974-68947993 None:intergenic 35.0%
CAAATGATGGTCTTCTCATA+TGG + chr3_4:68947978-68947997 Msa0517350:CDS 35.0%
GATTCAATGATGCTTGGATT+TGG - chr3_4:68948049-68948068 None:intergenic 35.0%
TGATCAGATTCAATGATGCT+TGG - chr3_4:68948055-68948074 None:intergenic 35.0%
TAGTGGTGATTATGTCAATG+AGG - chr3_4:68948171-68948190 None:intergenic 35.0%
ACATAATCACCACTATAGCT+TGG + chr3_4:68948176-68948195 Msa0517350:CDS 35.0%
CATAATCACCACTATAGCTT+GGG + chr3_4:68948177-68948196 Msa0517350:CDS 35.0%
TTTGCACAATATGCTTATGG+AGG + chr3_4:68948229-68948248 Msa0517350:CDS 35.0%
CTAGCAATTGTAGGTCTATT+AGG - chr3_4:68948262-68948281 None:intergenic 35.0%
ACAACTCATTGAACCATCAT+GGG + chr3_4:68948447-68948466 Msa0517350:CDS 35.0%
AAGGTTTAATGGGAGATTGA+AGG + chr3_4:68948501-68948520 Msa0517350:CDS 35.0%
! GAGCTAATGAAACCATTTTC+AGG + chr3_4:68948601-68948620 Msa0517350:CDS 35.0%
ATGAATGGTCGATGAACATG+TGG - chr3_4:68944815-68944834 None:intergenic 40.0%
TTTGTTGCCATCACAATGCA+AGG - chr3_4:68944968-68944987 None:intergenic 40.0%
!!! CAAACACTTGTTTTTGGAGC+TGG + chr3_4:68945090-68945109 Msa0517350:CDS 40.0%
AGAGAATGAGGTGCAATATG+AGG + chr3_4:68945444-68945463 Msa0517350:CDS 40.0%
!! ACCAGCAGATTTTGGAAATG+TGG + chr3_4:68945480-68945499 Msa0517350:CDS 40.0%
!! CCAGCAGATTTTGGAAATGT+GGG + chr3_4:68945481-68945500 Msa0517350:CDS 40.0%
TCCATCTTAGTTGTCGATCA+TGG + chr3_4:68945584-68945603 Msa0517350:CDS 40.0%
TCCATGATCGACAACTAAGA+TGG - chr3_4:68945588-68945607 None:intergenic 40.0%
CTTTGTAGGTGTATCATGCT+TGG - chr3_4:68945617-68945636 None:intergenic 40.0%
TTACCATCACAAACACCAAG+TGG + chr3_4:68945816-68945835 Msa0517350:CDS 40.0%
!! AATCCACTTGGTGTTTGTGA+TGG - chr3_4:68945822-68945841 None:intergenic 40.0%
CCTTGGATACGGATATTGTT+TGG - chr3_4:68945997-68946016 None:intergenic 40.0%
CCAAACAATATCCGTATCCA+AGG + chr3_4:68945994-68946013 Msa0517350:CDS 40.0%
!! GGTCGAAAGCATTCTAATAC+AGG + chr3_4:68946029-68946048 Msa0517350:CDS 40.0%
TTTCTATGTTCCTCGAGATG+AGG + chr3_4:68946331-68946350 Msa0517350:CDS 40.0%
GACTTTAATCAACCTGGGAA+TGG - chr3_4:68946558-68946577 None:intergenic 40.0%
! ATGGCCTCTGAGAAGTAAAT+TGG + chr3_4:68946946-68946965 Msa0517350:CDS 40.0%
GCATCCAATTTACTTCTCAG+AGG - chr3_4:68946953-68946972 None:intergenic 40.0%
TGGTTATACCACAGTTGAAG+AGG + chr3_4:68947033-68947052 Msa0517350:CDS 40.0%
TCCCTCACTTTGCTTACATA+TGG - chr3_4:68947191-68947210 None:intergenic 40.0%
TTGTTCTTCCTCACCAAACA+AGG + chr3_4:68947245-68947264 Msa0517350:CDS 40.0%
CTAGTGAGCTCAATGACTAT+TGG - chr3_4:68947281-68947300 None:intergenic 40.0%
! TGTGGTTTGTCGCCAATAAT+CGG - chr3_4:68947305-68947324 None:intergenic 40.0%
TTATTGGCGACAAACCACAA+TGG + chr3_4:68947306-68947325 Msa0517350:CDS 40.0%
!! CTACTAATCTTTGGCTTTGG+AGG + chr3_4:68947357-68947376 Msa0517350:CDS 40.0%
ACCATGAACTTATAAGCCAC+TGG + chr3_4:68947417-68947436 Msa0517350:CDS 40.0%
ACCAGTGGCTTATAAGTTCA+TGG - chr3_4:68947421-68947440 None:intergenic 40.0%
GATATGAATGTACTCACCAG+TGG - chr3_4:68947436-68947455 None:intergenic 40.0%
TCATATACTCACCGGAATCA+AGG - chr3_4:68947467-68947486 None:intergenic 40.0%
GCACTACTTCATATACTCAC+CGG - chr3_4:68947475-68947494 None:intergenic 40.0%
! GGATGCAGTAACTTGTAGAT+TGG - chr3_4:68947595-68947614 None:intergenic 40.0%
! TGCAAGGTATATCTGAGATG+TGG - chr3_4:68947616-68947635 None:intergenic 40.0%
ACCTTGCAGATAAATGCACT+TGG + chr3_4:68947628-68947647 Msa0517350:CDS 40.0%
TCCAAGTGCATTTATCTGCA+AGG - chr3_4:68947632-68947651 None:intergenic 40.0%
CTGTGGCATATGATAAGCTT+TGG + chr3_4:68947728-68947747 Msa0517350:CDS 40.0%
!! GCTTTGGCAATTTGATTTGC+AGG + chr3_4:68947744-68947763 Msa0517350:CDS 40.0%
TACCAAATGATCTTCTCCAC+CGG + chr3_4:68947770-68947789 Msa0517350:CDS 40.0%
ATACGTATAGACTTTACCGG+TGG - chr3_4:68947789-68947808 None:intergenic 40.0%
!! TTCTTTTAGAGGAATGGCTG+TGG + chr3_4:68947901-68947920 Msa0517350:intron 40.0%
GAAGATCCTAATGCACAACA+TGG + chr3_4:68947923-68947942 Msa0517350:CDS 40.0%
TTTAGGCCATGTTGTGCATT+AGG - chr3_4:68947932-68947951 None:intergenic 40.0%
! GACTACCCTTTTGCAAATGA+TGG + chr3_4:68947965-68947984 Msa0517350:CDS 40.0%
TGGACAGAAGTTCGAACAAA+AGG + chr3_4:68948091-68948110 Msa0517350:CDS 40.0%
GAAGTTCGAACAAAAGGTCA+CGG + chr3_4:68948097-68948116 Msa0517350:CDS 40.0%
ACAAATCAGAAGAACCATGG+TGG + chr3_4:68948122-68948141 Msa0517350:CDS 40.0%
AAGACCCTACAAAGGAAGAA+TGG + chr3_4:68948299-68948318 Msa0517350:CDS 40.0%
AGACCCTACAAAGGAAGAAT+GGG + chr3_4:68948300-68948319 Msa0517350:CDS 40.0%
TCTCCCATTCTTCCTTTGTA+GGG - chr3_4:68948306-68948325 None:intergenic 40.0%
TTCTCCCATTCTTCCTTTGT+AGG - chr3_4:68948307-68948326 None:intergenic 40.0%
! CAAACCTGAGCAAACACTTT+TGG + chr3_4:68948336-68948355 Msa0517350:CDS 40.0%
AGGTTGCTTGAATTTGCGAA+GGG - chr3_4:68948369-68948388 None:intergenic 40.0%
AAGGTTGCTTGAATTTGCGA+AGG - chr3_4:68948370-68948389 None:intergenic 40.0%
TTCGCAAATTCAAGCAACCT+TGG + chr3_4:68948369-68948388 Msa0517350:CDS 40.0%
GTTCAAAATGGTCATCACCA+AGG - chr3_4:68948389-68948408 None:intergenic 40.0%
AGTACTCTTCATCTGGTGAA+TGG - chr3_4:68948423-68948442 None:intergenic 40.0%
!! TTCACCAGATGAAGAGTACT+TGG + chr3_4:68948423-68948442 Msa0517350:CDS 40.0%
!! TCACCAGATGAAGAGTACTT+GGG + chr3_4:68948424-68948443 Msa0517350:CDS 40.0%
GACAACTCATTGAACCATCA+TGG + chr3_4:68948446-68948465 Msa0517350:CDS 40.0%
GGGAGATTGAAGGAAATTGA+AGG + chr3_4:68948511-68948530 Msa0517350:CDS 40.0%
TTGAAGAATAGGAATGGAGC+TGG + chr3_4:68948565-68948584 Msa0517350:CDS 40.0%
TGAAGAATAGGAATGGAGCT+GGG + chr3_4:68948566-68948585 Msa0517350:CDS 40.0%
!! ATGAAACCATTTTCAGGACC+TGG + chr3_4:68948607-68948626 Msa0517350:CDS 40.0%
! TGAAAGCTTTAGTGACGGTG+AGG + chr3_4:68945017-68945036 Msa0517350:CDS 45.0%
GGATATCATGCACATGTGTG+TGG - chr3_4:68945158-68945177 None:intergenic 45.0%
CCCACATTTCCAAAATCTGC+TGG - chr3_4:68945484-68945503 None:intergenic 45.0%
!! GTCCTTGATGGTTTTCCTGA+TGG + chr3_4:68945556-68945575 Msa0517350:CDS 45.0%
GTCCATCAGGAAAACCATCA+AGG - chr3_4:68945561-68945580 None:intergenic 45.0%
GATCGACAACTAAGATGGAC+AGG - chr3_4:68945583-68945602 None:intergenic 45.0%
GAACTAAGAGGGAATGGAGA+AGG + chr3_4:68945870-68945889 Msa0517350:CDS 45.0%
CTCAAAAGACCTGTTCTTGG+TGG + chr3_4:68945969-68945988 Msa0517350:CDS 45.0%
! TGAGGCGTTTGCTGAGATAA+AGG + chr3_4:68946349-68946368 Msa0517350:CDS 45.0%
GTCTGTGAGAATTGTGTCCA+AGG - chr3_4:68946429-68946448 None:intergenic 45.0%
! TCAACCTGGGAATGGCTTTT+TGG - chr3_4:68946550-68946569 None:intergenic 45.0%
GTTGGGAACTTCTGAATTGC+AGG + chr3_4:68946732-68946751 Msa0517350:intron 45.0%
!! GGAATTTGCTAGGGAGACTT+TGG + chr3_4:68946782-68946801 Msa0517350:CDS 45.0%
! TCTCTCGTGATAGCTGATTC+TGG - chr3_4:68946992-68947011 None:intergenic 45.0%
GTCATCGAGTCGCAGATAAT+TGG + chr3_4:68947013-68947032 Msa0517350:CDS 45.0%
GCTTAGTACCTCTTCAACTG+TGG - chr3_4:68947044-68947063 None:intergenic 45.0%
!! TCAATGTGCCTTGTTTGGTG+AGG - chr3_4:68947256-68947275 None:intergenic 45.0%
! TGGCTTCAATGTGCCTTGTT+TGG - chr3_4:68947261-68947280 None:intergenic 45.0%
GAGCTCACTAGACCGATTAT+TGG + chr3_4:68947290-68947309 Msa0517350:CDS 45.0%
TACCGGTGGAGAAGATCATT+TGG - chr3_4:68947775-68947794 None:intergenic 45.0%
CTGATCAAGAGCTTCAAGCA+TGG + chr3_4:68948068-68948087 Msa0517350:CDS 45.0%
ATCAAGAGCTTCAAGCATGG+TGG + chr3_4:68948071-68948090 Msa0517350:CDS 45.0%
GCGACAAATCAGAAGAACCA+TGG + chr3_4:68948119-68948138 Msa0517350:CDS 45.0%
! GTTTTCAGATTTGGCCACCA+TGG - chr3_4:68948139-68948158 None:intergenic 45.0%
AGATGCTACCCAAGCTATAG+TGG - chr3_4:68948188-68948207 None:intergenic 45.0%
TCCTTTGTAGGGTCTTCTGT+AGG - chr3_4:68948295-68948314 None:intergenic 45.0%
!! CACTCCAAAAGTGTTTGCTC+AGG - chr3_4:68948343-68948362 None:intergenic 45.0%
TGTCCCAAGTACTCTTCATC+TGG - chr3_4:68948430-68948449 None:intergenic 45.0%
TCTGGATTCTCATCCCATGA+TGG - chr3_4:68948463-68948482 None:intergenic 45.0%
!! TGGAAATGTGGGCGCTGTTT+TGG + chr3_4:68945492-68945511 Msa0517350:CDS 50.0%
CTAAGATGGACAGGTCCATC+AGG - chr3_4:68945574-68945593 None:intergenic 50.0%
!! TGTTTGGTGCCACCAAGAAC+AGG - chr3_4:68945981-68946000 None:intergenic 50.0%
GTACGACAACGCCTTGGATA+CGG - chr3_4:68946008-68946027 None:intergenic 50.0%
!! TATCCAAGGCGTTGTCGTAC+CGG + chr3_4:68946008-68946027 Msa0517350:CDS 50.0%
TGCTCCAAAAAGCCATTCCC+AGG + chr3_4:68946543-68946562 Msa0517350:CDS 50.0%
!! CAGGGGTATCAAAGCGCAAA+AGG - chr3_4:68946598-68946617 None:intergenic 50.0%
TTTGCTAGGGAGACTTTGGC+AGG + chr3_4:68946786-68946805 Msa0517350:CDS 50.0%
TTGCTAGGGAGACTTTGGCA+GGG + chr3_4:68946787-68946806 Msa0517350:CDS 50.0%
! CTCGTGATAGCTGATTCTGG+TGG - chr3_4:68946989-68947008 None:intergenic 50.0%
GCCTACAGAAGACCCTACAA+AGG + chr3_4:68948291-68948310 Msa0517350:CDS 50.0%
GTGACTCCAGGTCCTGAAAA+TGG - chr3_4:68948616-68948635 None:intergenic 50.0%
!! TTTTCAGGACCTGGAGTCAC+TGG + chr3_4:68948616-68948635 Msa0517350:CDS 50.0%
GTGACGGTGAGGCAAAGTGA+TGG + chr3_4:68945028-68945047 Msa0517350:CDS 55.0%
ACGGTGAGGCAAAGTGATGG+TGG + chr3_4:68945031-68945050 Msa0517350:CDS 55.0%
TCAGCAAACGCCTCATCTCG+AGG - chr3_4:68946344-68946363 None:intergenic 55.0%
! TTTTACTCCTGAGGACGCCG+TGG - chr3_4:68946393-68946412 None:intergenic 55.0%
TGCTAGGGAGACTTTGGCAG+GGG + chr3_4:68946788-68946807 Msa0517350:CDS 55.0%
AGGACCTGGAGTCACTGGAA+AGG + chr3_4:68948621-68948640 Msa0517350:CDS 55.0%
GGACCTGGAGTCACTGGAAA+GGG + chr3_4:68948622-68948641 Msa0517350:CDS 55.0%
ACTCCCTTTCCAGTGACTCC+AGG - chr3_4:68948628-68948647 None:intergenic 55.0%
AACACAACCACGGCGTCCTC+AGG + chr3_4:68946383-68946402 Msa0517350:CDS 60.0%
CGACCGGTACGACAACGCCT+TGG - chr3_4:68946014-68946033 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 68944723 68948851 68944723 ID=Msa0517350;Name=Msa0517350
chr3_4 mRNA 68944723 68948851 68944723 ID=Msa0517350-mRNA-1;Parent=Msa0517350;Name=Msa0517350-mRNA-1;_AED=0.08;_eAED=0.08;_QI=68|0.88|0.9|1|0.88|0.8|10|80|928
chr3_4 exon 68944723 68945133 68944723 ID=Msa0517350-mRNA-1:exon:18281;Parent=Msa0517350-mRNA-1
chr3_4 exon 68945389 68945657 68945389 ID=Msa0517350-mRNA-1:exon:18282;Parent=Msa0517350-mRNA-1
chr3_4 exon 68945810 68946050 68945810 ID=Msa0517350-mRNA-1:exon:18283;Parent=Msa0517350-mRNA-1
chr3_4 exon 68946303 68946629 68946303 ID=Msa0517350-mRNA-1:exon:18284;Parent=Msa0517350-mRNA-1
chr3_4 exon 68946754 68946848 68946754 ID=Msa0517350-mRNA-1:exon:18285;Parent=Msa0517350-mRNA-1
chr3_4 exon 68946944 68947054 68946944 ID=Msa0517350-mRNA-1:exon:18286;Parent=Msa0517350-mRNA-1
chr3_4 exon 68947137 68947438 68947137 ID=Msa0517350-mRNA-1:exon:18287;Parent=Msa0517350-mRNA-1
chr3_4 exon 68947525 68947791 68947525 ID=Msa0517350-mRNA-1:exon:18288;Parent=Msa0517350-mRNA-1
chr3_4 exon 68947910 68948668 68947910 ID=Msa0517350-mRNA-1:exon:18289;Parent=Msa0517350-mRNA-1
chr3_4 exon 68948699 68948851 68948699 ID=Msa0517350-mRNA-1:exon:18290;Parent=Msa0517350-mRNA-1
chr3_4 five_prime_UTR 68944723 68944790 68944723 ID=Msa0517350-mRNA-1:five_prime_utr;Parent=Msa0517350-mRNA-1
chr3_4 CDS 68944791 68945133 68944791 ID=Msa0517350-mRNA-1:cds;Parent=Msa0517350-mRNA-1
chr3_4 CDS 68945389 68945657 68945389 ID=Msa0517350-mRNA-1:cds;Parent=Msa0517350-mRNA-1
chr3_4 CDS 68945810 68946050 68945810 ID=Msa0517350-mRNA-1:cds;Parent=Msa0517350-mRNA-1
chr3_4 CDS 68946303 68946629 68946303 ID=Msa0517350-mRNA-1:cds;Parent=Msa0517350-mRNA-1
chr3_4 CDS 68946754 68946848 68946754 ID=Msa0517350-mRNA-1:cds;Parent=Msa0517350-mRNA-1
chr3_4 CDS 68946944 68947054 68946944 ID=Msa0517350-mRNA-1:cds;Parent=Msa0517350-mRNA-1
chr3_4 CDS 68947137 68947438 68947137 ID=Msa0517350-mRNA-1:cds;Parent=Msa0517350-mRNA-1
chr3_4 CDS 68947525 68947791 68947525 ID=Msa0517350-mRNA-1:cds;Parent=Msa0517350-mRNA-1
chr3_4 CDS 68947910 68948668 68947910 ID=Msa0517350-mRNA-1:cds;Parent=Msa0517350-mRNA-1
chr3_4 CDS 68948699 68948771 68948699 ID=Msa0517350-mRNA-1:cds;Parent=Msa0517350-mRNA-1
chr3_4 three_prime_UTR 68948772 68948851 68948772 ID=Msa0517350-mRNA-1:three_prime_utr;Parent=Msa0517350-mRNA-1
Gene Sequence

>Msa0517350

ATGACGCTTTTGTTGAAGAAACCACATGTTCATCGACCATTCATCATTAACAATGCATCTTCACCTTCCTATATAAGGAGAAAATATTCTCATAGTTGCTACAACAAAAGTAATTGGTTCTTGAAATCTTCTTTGTCTCTAATTAGTACTAGTACAAGTAAAAAGGTTGTTTATCCTTGCATTGTGATGGCAACAAAAAGTAGCACCGAGTCTAAATCTCATAATGTGAAAGCTTTAGTGACGGTGAGGCAAAGTGATGGTGGTCTCATCAAGAATATTGTTACTGGTATTGTTGGTAACAAACACTTGTTTTTGGAGCTGGTTAGTGCTGAACTTGATCCAAGAACAAATTCAGAGAAAAAGACAATAAAAGGAAAAGCGCACGAAACTGAGAAAAAAGAGAATGAGGTGCAATATGAGGCAACATTTGAGTTACCAGCAGATTTTGGAAATGTGGGCGCTGTTTTGGTTGAGAATGAGCATGACAAAGAGATATTTCTAAATAACATAGTCCTTGATGGTTTTCCTGATGGACCTGTCCATCTTAGTTGTCGATCATGGATTCAACCCAAGCATGATACACCTACAAAGAGAGTTTTCTTCACTAACAAGATGTATTTACCATCACAAACACCAAGTGGATTAAGAAAGCTTAGAGAAGATGAGCTAATAGAACTAAGAGGGAATGGAGAAGGAGAAAGAAAAAGCTCAGATAGAATTTATGACTATGATGTCTACAATGATCTTGGAGATCCTGAGACCAACATTAAACTCAAAAGACCTGTTCTTGGTGGCACCAAACAATATCCGTATCCAAGGCGTTGTCGTACCGGTCGAAAGCATTCTAATACAGATCCATCATACGAAAAGAGAGATTCGGATTTCTATGTTCCTCGAGATGAGGCGTTTGCTGAGATAAAGGAGATACAATTCAACACAACCACGGCGTCCTCAGGAGTAAAAACAATTTTGGAATCCTTGGACACAATTCTCACAGACATAAATCTTGGATTTGACAGCTTTGAAGACATAGACGAACTTTACAAAGAAGGATTTCAATTACCATCATCTCTTGGATCCAATAATTTAACATTGCTCCAAAAAGCCATTCCCAGGTTGATTAAAGTCGCTAATGACAGCCAAAACCTTTTGCGCTTTGATACCCCTGAAGCACTTAAAAGAGACAAATTCTTTTGGCTATCTGATGAGGAATTTGCTAGGGAGACTTTGGCAGGGGCCAACCCAAATAGCATCCAATTAGTCAAGGCAATAATTGAATGGCCTCTGAGAAGTAAATTGGATGCCCAAATTTATGGTCCACCAGAATCAGCTATCACGAGAGAAGTCATCGAGTCGCAGATAATTGGTTATACCACAGTTGAAGAGGCCATTCAAGAGAAGAAATTGTTTGTACTAGACTACCATGATTTATATTTACCATATGTAAGCAAAGTGAGGGAAATGAAGAATACTACACTGTATGGATCAAGAACATTGTTCTTCCTCACCAAACAAGGCACATTGAAGCCAATAGTCATTGAGCTCACTAGACCGATTATTGGCGACAAACCACAATGGAAACAAATATTCACTCCTGCTTCTGATTCTACTAATCTTTGGCTTTGGAGGCTTGCAAAAGCTCATGTTCTTACTCATGATACTGGTTACCATGAACTTATAAGCCACTGGTTAAGGACTCACTGTGTTGTAGAACCCTTTGTGATTGCAACAAATAGGCAACTTAGCACCATGCATCCAATCTACAAGTTACTGCATCCACATCTCAGATATACCTTGCAGATAAATGCACTTGGACGCGAGATTCTTATTAGTTCATATGGAGTCATTGAAAGCACATTCTTTACTAAAAAGTATTCAATGGAATTGAGTTCTGTGGCATATGATAAGCTTTGGCAATTTGATTTGCAGGGACTACCAAATGATCTTCTCCACCGAGGAATGGCTGTGGAAGATCCTAATGCACAACATGGCCTAAAGCTAGCAATAGAAGACTACCCTTTTGCAAATGATGGTCTTCTCATATGGGATGCAATTAAACAATGGGTGACAGATTATGTCAATCATTACTATCCAAATCCAAGCATCATTGAATCTGATCAAGAGCTTCAAGCATGGTGGACAGAAGTTCGAACAAAAGGTCACGGCGACAAATCAGAAGAACCATGGTGGCCAAATCTGAAAACACAAAAAGACCTCATTGACATAATCACCACTATAGCTTGGGTAGCATCTGCACATCATTCAGCTACAAACTTTGCACAATATGCTTATGGAGGCTATTTCCCTAATAGACCTACAATTGCTAGAAACAACATGCCTACAGAAGACCCTACAAAGGAAGAATGGGAGAAATTTCTGAACAAACCTGAGCAAACACTTTTGGAGTGTTTCCCTTCGCAAATTCAAGCAACCTTGGTGATGACCATTTTGAACTTATTATCATACCATTCACCAGATGAAGAGTACTTGGGACAACTCATTGAACCATCATGGGATGAGAATCCAGATATAAAGATAGCATTTGAAAGGTTTAATGGGAGATTGAAGGAAATTGAAGGTATAATAGATTCAAGAAATGCAAATACTAATTTGAAGAATAGGAATGGAGCTGGGATAGTACCTTATGAGCTAATGAAACCATTTTCAGGACCTGGAGTCACTGGAAAGGGAGTTCCATATAGCATATCTATTTATACAGTTGTACTACAACAATATGTCCTATATATCACATTTCCACAATTACTTTTGTTGTTGTTTTTAGAGTAA
Protein sequence

>Msa0517350

MTLLLKKPHVHRPFIINNASSPSYIRRKYSHSCYNKSNWFLKSSLSLISTSTSKKVVYPCIVMATKSSTESKSHNVKALVTVRQSDGGLIKNIVTGIVGNKHLFLELVSAELDPRTNSEKKTIKGKAHETEKKENEVQYEATFELPADFGNVGAVLVENEHDKEIFLNNIVLDGFPDGPVHLSCRSWIQPKHDTPTKRVFFTNKMYLPSQTPSGLRKLREDELIELRGNGEGERKSSDRIYDYDVYNDLGDPETNIKLKRPVLGGTKQYPYPRRCRTGRKHSNTDPSYEKRDSDFYVPRDEAFAEIKEIQFNTTTASSGVKTILESLDTILTDINLGFDSFEDIDELYKEGFQLPSSLGSNNLTLLQKAIPRLIKVANDSQNLLRFDTPEALKRDKFFWLSDEEFARETLAGANPNSIQLVKAIIEWPLRSKLDAQIYGPPESAITREVIESQIIGYTTVEEAIQEKKLFVLDYHDLYLPYVSKVREMKNTTLYGSRTLFFLTKQGTLKPIVIELTRPIIGDKPQWKQIFTPASDSTNLWLWRLAKAHVLTHDTGYHELISHWLRTHCVVEPFVIATNRQLSTMHPIYKLLHPHLRYTLQINALGREILISSYGVIESTFFTKKYSMELSSVAYDKLWQFDLQGLPNDLLHRGMAVEDPNAQHGLKLAIEDYPFANDGLLIWDAIKQWVTDYVNHYYPNPSIIESDQELQAWWTEVRTKGHGDKSEEPWWPNLKTQKDLIDIITTIAWVASAHHSATNFAQYAYGGYFPNRPTIARNNMPTEDPTKEEWEKFLNKPEQTLLECFPSQIQATLVMTILNLLSYHSPDEEYLGQLIEPSWDENPDIKIAFERFNGRLKEIEGIIDSRNANTNLKNRNGAGIVPYELMKPFSGPGVTGKGVPYSISIYTVVLQQYVLYITFPQLLLLLFLE*