Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0421160 | AES71835.1 | 90.909 | 66 | 4 | 1 | 1 | 66 | 1 | 64 | 3.58e-32 | 115 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0421160 | G7J9J6 | 90.909 | 66 | 4 | 1 | 1 | 66 | 1 | 64 | 1.71e-32 | 115 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0044710 | Msa0421160 | 0.804106 | 2.532822e-49 | -8.615850e-47 |
| Msa0071240 | Msa0421160 | 0.828538 | 8.502309e-55 | -8.615850e-47 |
| Msa0421160 | Msa0555950 | 0.835376 | 1.739954e-56 | -8.615850e-47 |
| Msa0421160 | Msa0610370 | 0.831944 | 1.252347e-55 | -8.615850e-47 |
| Msa0421160 | Msa0650900 | 0.827789 | 1.288282e-54 | -8.615850e-47 |
| Msa0421160 | Msa0834600 | 0.803791 | 2.944885e-49 | -8.615850e-47 |
| Msa0421160 | Msa1174870 | 0.806737 | 7.112563e-50 | -8.615850e-47 |
| Msa0421160 | Msa1187520 | 0.820741 | 5.843028e-53 | -8.615850e-47 |
| Msa0421160 | Msa1233500 | 0.801545 | 8.559864e-49 | -8.615850e-47 |
| Msa0421160 | Msa1282920 | 0.822654 | 2.110257e-53 | -8.615850e-47 |
| Msa0421160 | Msa1322190 | 0.804207 | 2.413005e-49 | -8.615850e-47 |
| Msa0421160 | Msa1378460 | 0.800296 | 1.540475e-48 | -8.615850e-47 |
| Msa0326670 | Msa0421160 | 0.808053 | 3.741713e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0421160 | MtrunA17_Chr3g0121081 | 90.909 | 66 | 4 | 1 | 1 | 66 | 1 | 64 | 3.30e-36 | 115 |
| Msa0421160 | MtrunA17_Chr2g0281351 | 66.667 | 63 | 15 | 3 | 1 | 63 | 1 | 57 | 1.79e-18 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCAACGGTGGAGTATT+AGG | 0.287872 | 3_2:+70051623 | Msa0421160:CDS |
| AGAAGGAGGACCACATGATA+TGG | 0.373358 | 3_2:-70051744 | None:intergenic |
| ACCTGACCGCGCTTTGGAAT+CGG | 0.400882 | 3_2:-70051665 | None:intergenic |
| ATCATCACCTGACCGCGCTT+TGG | 0.406692 | 3_2:-70051671 | None:intergenic |
| ACTAAAAGCTGGACTCTAGA+AGG | 0.426432 | 3_2:-70051761 | None:intergenic |
| TCCGATTCCAAAGCGCGGTC+AGG | 0.447982 | 3_2:+70051664 | Msa0421160:CDS |
| AAGCGCGGTCAGGTGATGAT+TGG | 0.458553 | 3_2:+70051674 | Msa0421160:CDS |
| TTACATATTAAACTAAAAGC+TGG | 0.482150 | 3_2:-70051772 | None:intergenic |
| GTGATGATTGGAATTGTGTT+GGG | 0.488885 | 3_2:+70051686 | Msa0421160:CDS |
| GATTGGAATTGTGTTGGGAT+TGG | 0.504801 | 3_2:+70051691 | Msa0421160:CDS |
| GGTGATGATTGGAATTGTGT+TGG | 0.535256 | 3_2:+70051685 | Msa0421160:CDS |
| GAAGATGCAAACAACTGAGT+TGG | 0.572193 | 3_2:-70051714 | None:intergenic |
| TCAACGGTGGAGTATTAGGA+AGG | 0.608387 | 3_2:+70051627 | Msa0421160:CDS |
| AAAAGCAAAGAGAAGATGGA+GGG | 0.628558 | 3_2:+70051569 | None:intergenic |
| TTCATACGTTCCATATCATG+TGG | 0.638393 | 3_2:+70051734 | Msa0421160:CDS |
| ACTGCAATCATGAAAATCAA+CGG | 0.647400 | 3_2:+70051611 | Msa0421160:CDS |
| GACCGCGCTTTGGAATCGGA+CGG | 0.688444 | 3_2:-70051661 | None:intergenic |
| AAAAGCTGGACTCTAGAAGG+AGG | 0.691729 | 3_2:-70051758 | None:intergenic |
| GCAATCATGAAAATCAACGG+TGG | 0.699924 | 3_2:+70051614 | Msa0421160:CDS |
| GACCGTCCGATTCCAAAGCG+CGG | 0.710213 | 3_2:+70051659 | Msa0421160:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTGCAATCATGAAAATCAA+CGG | + | chr3_2:70051611-70051630 | Msa0421160:CDS | 30.0% | |
| ! | AAAATCAACGGTGGAGTATT+AGG | + | chr3_2:70051623-70051642 | Msa0421160:CDS | 35.0% |
| GTGATGATTGGAATTGTGTT+GGG | + | chr3_2:70051686-70051705 | Msa0421160:CDS | 35.0% | |
| TTCATACGTTCCATATCATG+TGG | + | chr3_2:70051734-70051753 | Msa0421160:CDS | 35.0% | |
| GCAATCATGAAAATCAACGG+TGG | + | chr3_2:70051614-70051633 | Msa0421160:CDS | 40.0% | |
| GGTGATGATTGGAATTGTGT+TGG | + | chr3_2:70051685-70051704 | Msa0421160:CDS | 40.0% | |
| GATTGGAATTGTGTTGGGAT+TGG | + | chr3_2:70051691-70051710 | Msa0421160:CDS | 40.0% | |
| GAAGATGCAAACAACTGAGT+TGG | - | chr3_2:70051717-70051736 | None:intergenic | 40.0% | |
| ACTAAAAGCTGGACTCTAGA+AGG | - | chr3_2:70051764-70051783 | None:intergenic | 40.0% | |
| ! | TCAACGGTGGAGTATTAGGA+AGG | + | chr3_2:70051627-70051646 | Msa0421160:CDS | 45.0% |
| AGAAGGAGGACCACATGATA+TGG | - | chr3_2:70051747-70051766 | None:intergenic | 45.0% | |
| ! | AAAAGCTGGACTCTAGAAGG+AGG | - | chr3_2:70051761-70051780 | None:intergenic | 45.0% |
| !! | ACCTGACCGCGCTTTGGAAT+CGG | - | chr3_2:70051668-70051687 | None:intergenic | 55.0% |
| ATCATCACCTGACCGCGCTT+TGG | - | chr3_2:70051674-70051693 | None:intergenic | 55.0% | |
| AAGCGCGGTCAGGTGATGAT+TGG | + | chr3_2:70051674-70051693 | Msa0421160:CDS | 55.0% | |
| GACCGTCCGATTCCAAAGCG+CGG | + | chr3_2:70051659-70051678 | Msa0421160:CDS | 60.0% | |
| !! | GACCGCGCTTTGGAATCGGA+CGG | - | chr3_2:70051664-70051683 | None:intergenic | 60.0% |
| TCCGATTCCAAAGCGCGGTC+AGG | + | chr3_2:70051664-70051683 | Msa0421160:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 70051584 | 70051787 | 70051584 | ID=Msa0421160;Name=Msa0421160 |
| chr3_2 | mRNA | 70051584 | 70051787 | 70051584 | ID=Msa0421160-mRNA-1;Parent=Msa0421160;Name=Msa0421160-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|-1|0|1|-1|1|1|0|67 |
| chr3_2 | exon | 70051584 | 70051787 | 70051584 | ID=Msa0421160-mRNA-1:exon:16727;Parent=Msa0421160-mRNA-1 |
| chr3_2 | CDS | 70051584 | 70051787 | 70051584 | ID=Msa0421160-mRNA-1:cds;Parent=Msa0421160-mRNA-1 |
| Gene Sequence |
| Protein sequence |
