Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0555950 | AES90005.1 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 1.02e-53 | 172 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0012040 | Msa0555950 | 0.806391 | 8.414522e-50 | -8.615850e-47 |
| Msa0030380 | Msa0555950 | 0.801337 | 9.444484e-49 | -8.615850e-47 |
| Msa0031050 | Msa0555950 | 0.802341 | 5.873829e-49 | -8.615850e-47 |
| Msa0031090 | Msa0555950 | 0.803579 | 3.259129e-49 | -8.615850e-47 |
| Msa0032610 | Msa0555950 | 0.800046 | 1.731508e-48 | -8.615850e-47 |
| Msa0033340 | Msa0555950 | 0.811215 | 7.825393e-51 | -8.615850e-47 |
| Msa0044710 | Msa0555950 | 0.835586 | 1.539022e-56 | -8.615850e-47 |
| Msa0057140 | Msa0555950 | 0.806584 | 7.663842e-50 | -8.615850e-47 |
| Msa0071240 | Msa0555950 | 0.824315 | 8.626433e-54 | -8.615850e-47 |
| Msa0078430 | Msa0555950 | 0.825593 | 4.306661e-54 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0555950 | MtrunA17_Chr4g0043191 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 9.42e-58 | 172 |
| Msa0555950 | MtrunA17_Chr4g0043181 | 80.899 | 89 | 13 | 1 | 1 | 89 | 1 | 85 | 5.05e-40 | 127 |
| Msa0555950 | MtrunA17_Chr8g0376661 | 79.545 | 88 | 18 | 0 | 1 | 88 | 1 | 88 | 1.65e-36 | 118 |
| Msa0555950 | MtrunA17_Chr5g0434291 | 77.528 | 89 | 20 | 0 | 1 | 89 | 1 | 89 | 2.91e-36 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0555950 | AT1G30260.1 | 60.417 | 48 | 18 | 1 | 1 | 48 | 1 | 47 | 3.72e-15 | 65.1 |
Find 12 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAACTGCATTGGTAGAAG+AGG | 0.557920 | 4_1:-53961656 | None:intergenic |
| AACAATCACTGAAGAGGATA+TGG | 0.331678 | 4_1:+53961558 | Msa0555950:CDS |
| ATCACGCTAACATACTGTGG+TGG | 0.690677 | 4_1:-53961508 | None:intergenic |
| CACAGTATGTTAGCGTGATG+AGG | 0.569645 | 4_1:+53961512 | Msa0555950:CDS |
| CCAATGCAGTTGTTAATTCT+AGG | 0.257861 | 4_1:+53961665 | Msa0555950:CDS |
| CCTAGAATTAACAACTGCAT+TGG | 0.551602 | 4_1:-53961665 | None:intergenic |
| CTCATCACGCTAACATACTG+TGG | 0.654266 | 4_1:-53961511 | None:intergenic |
| GATGGAAACAATCACTGAAG+AGG | 0.591147 | 4_1:+53961552 | Msa0555950:CDS |
| GCGTAGAACATCAAAGATGA+TGG | 0.510830 | 4_1:+53961534 | Msa0555950:CDS |
| GTGTTCTATGAACTTCCTTC+AGG | 0.381163 | 4_1:-53961693 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAATCACTGAAGAGGATA+TGG | + | chr4_1:53961558-53961577 | Msa0555950:CDS | 35.0% | |
| CCTAGAATTAACAACTGCAT+TGG | - | chr4_1:53961668-53961687 | None:intergenic | 35.0% | |
| CCAATGCAGTTGTTAATTCT+AGG | + | chr4_1:53961665-53961684 | Msa0555950:CDS | 35.0% | |
| TAATTCTAGGTACTTCCTGA+AGG | + | chr4_1:53961678-53961697 | Msa0555950:CDS | 35.0% | |
| GCGTAGAACATCAAAGATGA+TGG | + | chr4_1:53961534-53961553 | Msa0555950:CDS | 40.0% | |
| GATGGAAACAATCACTGAAG+AGG | + | chr4_1:53961552-53961571 | Msa0555950:CDS | 40.0% | |
| GTGTTCTATGAACTTCCTTC+AGG | - | chr4_1:53961696-53961715 | None:intergenic | 40.0% | |
| ATCACGCTAACATACTGTGG+TGG | - | chr4_1:53961511-53961530 | None:intergenic | 45.0% | |
| CTCATCACGCTAACATACTG+TGG | - | chr4_1:53961514-53961533 | None:intergenic | 45.0% | |
| CACAGTATGTTAGCGTGATG+AGG | + | chr4_1:53961512-53961531 | Msa0555950:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 53961466 | 53961735 | 53961466 | ID=Msa0555950;Name=Msa0555950 |
| chr4_1 | mRNA | 53961466 | 53961735 | 53961466 | ID=Msa0555950-mRNA-1;Parent=Msa0555950;Name=Msa0555950-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|-1|0|1|-1|1|1|0|89 |
| chr4_1 | exon | 53961466 | 53961735 | 53961466 | ID=Msa0555950-mRNA-1:exon:15776;Parent=Msa0555950-mRNA-1 |
| chr4_1 | CDS | 53961466 | 53961735 | 53961466 | ID=Msa0555950-mRNA-1:cds;Parent=Msa0555950-mRNA-1 |
| Gene Sequence |
>Msa0555950 ATGGCGAATTCTCGCATTGCAAGGTTTTTCATGGAGGTGGCACCACCACAGTATGTTAGCGTGATGAGGCGTAGAACATCAAAGATGATGGAAACAATCACTGAAGAGGATATGGAAATGAGTTCAAATGATAGTGTAATTCCACCACCTAAAAGTTCATCAACTTCATCACTTGCTGCTTCTGCATGTGCCTCTTCTACCAATGCAGTTGTTAATTCTAGGTACTTCCTGAAGGAAGTTCATAGAACACTCTCAAGTTTGAATCACTGA |
| Protein sequence |
>Msa0555950 MANSRIARFFMEVAPPQYVSVMRRRTSKMMETITEEDMEMSSNDSVIPPPKSSSTSSLAASACASSTNAVVNSRYFLKEVHRTLSSLNH* |
