AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1378460


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1378460 MtrunA17_Chr4g0019521 63.380 71 21 1 3 68 2 72 7.33e-26 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCACTCTTGGTATAGAATT+AGG 0.267818 tig0020061:+50278 Msa1378460:CDS
CTTCTACCTTGGCAAGATAA+TGG 0.344639 tig0020061:-50313 None:intergenic
GTATAGAATTAGGACCCCTT+TGG 0.412469 tig0020061:+50288 Msa1378460:CDS
CACTCACCATTAGCCACTCT+TGG 0.472156 tig0020061:+50266 Msa1378460:CDS
AAGAAAGACATAGGAGGAAG+AGG 0.481981 tig0020061:+50367 Msa1378460:CDS
AGATGCATGGTCTTCTACCT+TGG 0.492293 tig0020061:-50324 None:intergenic
TCTATACCAAGAGTGGCTAA+TGG 0.502607 tig0020061:-50272 None:intergenic
GGCAAGATAATGGAACCAAA+GGG 0.513637 tig0020061:-50303 None:intergenic
TTGGTTCCATTATCTTGCCA+AGG 0.539176 tig0020061:+50307 Msa1378460:CDS
CATGAAGAAAAGAAAGACAT+AGG 0.556728 tig0020061:+50358 Msa1378460:CDS
TGGCAAGATAATGGAACCAA+AGG 0.571857 tig0020061:-50304 None:intergenic
TCCTAATTCTATACCAAGAG+TGG 0.590163 tig0020061:-50279 None:intergenic
TGATTGGAGTTAGAGATGCA+TGG 0.614307 tig0020061:-50337 None:intergenic
GAAGAAAAGAAAGACATAGG+AGG 0.619275 tig0020061:+50361 Msa1378460:CDS
GCAAGATAATGGAACCAAAG+GGG 0.711481 tig0020061:-50302 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTCTTTCTTTTCTTCATGAT+TGG - tig0020061:50356-50375 None:intergenic 30.0%
CATGAAGAAAAGAAAGACAT+AGG + tig0020061:50358-50377 Msa1378460:CDS 30.0%
TCCTAATTCTATACCAAGAG+TGG - tig0020061:50282-50301 None:intergenic 35.0%
GAAGAAAAGAAAGACATAGG+AGG + tig0020061:50361-50380 Msa1378460:CDS 35.0%
TCTATACCAAGAGTGGCTAA+TGG - tig0020061:50275-50294 None:intergenic 40.0%
GCCACTCTTGGTATAGAATT+AGG + tig0020061:50278-50297 Msa1378460:CDS 40.0%
GTATAGAATTAGGACCCCTT+TGG + tig0020061:50288-50307 Msa1378460:CDS 40.0%
!! GCAAGATAATGGAACCAAAG+GGG - tig0020061:50305-50324 None:intergenic 40.0%
!! GGCAAGATAATGGAACCAAA+GGG - tig0020061:50306-50325 None:intergenic 40.0%
!! TGGCAAGATAATGGAACCAA+AGG - tig0020061:50307-50326 None:intergenic 40.0%
TTGGTTCCATTATCTTGCCA+AGG + tig0020061:50307-50326 Msa1378460:CDS 40.0%
CTTCTACCTTGGCAAGATAA+TGG - tig0020061:50316-50335 None:intergenic 40.0%
TGATTGGAGTTAGAGATGCA+TGG - tig0020061:50340-50359 None:intergenic 40.0%
AAGAAAGACATAGGAGGAAG+AGG + tig0020061:50367-50386 Msa1378460:CDS 40.0%
!!! TGAAAAGCACTTTTCGCATC+AGG + tig0020061:50423-50442 Msa1378460:CDS 40.0%
AGATGCATGGTCTTCTACCT+TGG - tig0020061:50327-50346 None:intergenic 45.0%
!! AAAGCACTTTTCGCATCAGG+TGG + tig0020061:50426-50445 Msa1378460:CDS 45.0%
CACTCACCATTAGCCACTCT+TGG + tig0020061:50266-50285 Msa1378460:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0020061 gene 50259 50465 50259 ID=Msa1378460;Name=Msa1378460
tig0020061 mRNA 50259 50465 50259 ID=Msa1378460-mRNA-1;Parent=Msa1378460;Name=Msa1378460-mRNA-1;_AED=0.54;_eAED=0.55;_QI=0|-1|0|1|-1|1|1|0|68
tig0020061 exon 50259 50465 50259 ID=Msa1378460-mRNA-1:exon:6068;Parent=Msa1378460-mRNA-1
tig0020061 CDS 50259 50465 50259 ID=Msa1378460-mRNA-1:cds;Parent=Msa1378460-mRNA-1
Gene Sequence

>Msa1378460

ATGGCATCACTCACCATTAGCCACTCTTGGTATAGAATTAGGACCCCTTTGGTTCCATTATCTTGCCAAGGTAGAAGACCATGCATCTCTAACTCCAATCATGAAGAAAAGAAAGACATAGGAGGAAGAGGAGCACTACTACAGAAAGTATTTTTCACATCAGTTGAAAAGCACTTTTCGCATCAGGTGGCAACTGATGTAAAGTGA

Protein sequence

>Msa1378460

MASLTISHSWYRIRTPLVPLSCQGRRPCISNSNHEEKKDIGGRGALLQKVFFTSVEKHFSHQVATDVK*