AlfalfaGEDB | Msa1174870

M. sativa cultivar zhongmu-4 / Msa1174870

KEGG: K09512
GO: GO:0003674 GO:0005488 GO:0005515 GO:0051087
PFAM: PF00226.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174870	XP_003627374.1	92.827	237	15	2	1	237	1	235	1.06e-145	416

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174870	sp\|Q4R7Y5\|DNJB6_MACFA	49.275	69	30	1	10	78	3	66	1.73e-15	76.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174870	G7L8C9	92.827	237	15	2	1	237	1	235	5.08e-146	416

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006040	Msa1174870	0.876114	1.806086e-68	-8.615850e-47
Msa0032610	Msa1174870	0.826002	3.445240e-54	-8.615850e-47
Msa0036160	Msa1174870	0.841251	5.321785e-58	-8.615850e-47
Msa0042980	Msa1174870	-0.828183	1.035703e-54	-8.615850e-47
Msa0054750	Msa1174870	0.865372	6.195731e-65	-8.615850e-47
Msa0111310	Msa1174870	0.812100	5.025369e-51	-8.615850e-47
Msa0615270	Msa1174870	-0.819728	9.971165e-53	-8.615850e-47
Msa0629650	Msa1174870	0.812587	3.933234e-51	-8.615850e-47
Msa0650730	Msa1174870	0.802912	4.478044e-49	-8.615850e-47
Msa0654010	Msa1174870	0.816023	6.845847e-52	-8.615850e-47
Msa0661210	Msa1174870	0.867927	9.535632e-66	-8.615850e-47
Msa0684850	Msa1174870	0.824009	1.017934e-53	-8.615850e-47
Msa0698310	Msa1174870	-0.810334	1.213930e-50	-8.615850e-47
Msa0709670	Msa1174870	0.804512	2.084309e-49	-8.615850e-47
Msa0713230	Msa1174870	0.806563	7.741152e-50	-8.615850e-47
Msa0722910	Msa1174870	0.808368	3.204894e-50	-8.615850e-47
Msa0722920	Msa1174870	0.812581	3.945726e-51	-8.615850e-47
Msa0723130	Msa1174870	0.829829	4.134328e-55	-8.615850e-47
Msa0739680	Msa1174870	0.816922	4.308210e-52	-8.615850e-47
Msa0755080	Msa1174870	0.863429	2.504870e-64	-8.615850e-47
Msa0782470	Msa1174870	0.822225	2.655006e-53	-8.615850e-47
Msa0782490	Msa1174870	0.809540	1.799491e-50	-8.615850e-47
Msa0795920	Msa1174870	0.840120	1.052654e-57	-8.615850e-47
Msa0796950	Msa1174870	0.833716	4.544306e-56	-8.615850e-47
Msa0797970	Msa1174870	0.858573	7.508240e-63	-8.615850e-47
Msa0801360	Msa1174870	0.822254	2.613924e-53	-8.615850e-47
Msa0834450	Msa1174870	0.862902	3.646801e-64	-8.615850e-47
Msa0834460	Msa1174870	0.810940	8.977929e-51	-8.615850e-47
Msa0839830	Msa1174870	0.820169	7.904671e-53	-8.615850e-47
Msa0125640	Msa1174870	-0.805762	1.141509e-49	-8.615850e-47
Msa0136890	Msa1174870	-0.817680	2.907916e-52	-8.615850e-47
Msa0148120	Msa1174870	0.845655	3.547042e-59	-8.615850e-47
Msa0172780	Msa1174870	0.808625	2.824490e-50	-8.615850e-47
Msa0177300	Msa1174870	0.800175	1.630229e-48	-8.615850e-47
Msa0197180	Msa1174870	0.845415	4.119946e-59	-8.615850e-47
Msa0221960	Msa1174870	-0.818292	2.114755e-52	-8.615850e-47
Msa0223380	Msa1174870	0.805554	1.262128e-49	-8.615850e-47
Msa0376340	Msa1174870	0.803782	2.957816e-49	-8.615850e-47
Msa0379410	Msa1174870	0.830220	3.319187e-55	-8.615850e-47
Msa0380040	Msa1174870	0.810199	1.297954e-50	-8.615850e-47
Msa0381410	Msa1174870	0.812251	4.658177e-51	-8.615850e-47
Msa0389810	Msa1174870	0.841348	5.017590e-58	-8.615850e-47
Msa0394340	Msa1174870	0.834027	3.799705e-56	-8.615850e-47
Msa0421160	Msa1174870	0.806737	7.112563e-50	-8.615850e-47
Msa0429290	Msa1174870	0.805068	1.595704e-49	-8.615850e-47
Msa0442200	Msa1174870	0.817001	4.134102e-52	-8.615850e-47
Msa0457510	Msa1174870	0.818010	2.449503e-52	-8.615850e-47
Msa0468130	Msa1174870	0.847345	1.226707e-59	-8.615850e-47
Msa0475240	Msa1174870	0.827990	1.152506e-54	-8.615850e-47
Msa0475580	Msa1174870	0.817284	3.572377e-52	-8.615850e-47
Msa0482190	Msa1174870	-0.803159	3.982881e-49	-8.615850e-47
Msa0982270	Msa1174870	0.843115	1.708332e-58	-8.615850e-47
Msa1006850	Msa1174870	0.801912	7.196681e-49	-8.615850e-47
Msa1012830	Msa1174870	0.807885	4.061870e-50	-8.615850e-47
Msa1012920	Msa1174870	0.826621	2.452989e-54	-8.615850e-47
Msa1021340	Msa1174870	0.822423	2.387671e-53	-8.615850e-47
Msa1037020	Msa1174870	0.814375	1.590473e-51	-8.615850e-47
Msa1054410	Msa1174870	0.858650	7.122065e-63	-8.615850e-47
Msa1077670	Msa1174870	0.835309	1.808516e-56	-8.615850e-47
Msa1079730	Msa1174870	-0.846832	1.694723e-59	-8.615850e-47
Msa1089040	Msa1174870	0.802730	4.883043e-49	-8.615850e-47
Msa1094430	Msa1174870	0.801121	1.045522e-48	-8.615850e-47
Msa0238940	Msa1174870	0.803845	2.870068e-49	-8.615850e-47
Msa0265380	Msa1174870	0.823818	1.128737e-53	-8.615850e-47
Msa0268090	Msa1174870	0.842534	2.439136e-58	-8.615850e-47
Msa0303360	Msa1174870	0.831972	1.232940e-55	-8.615850e-47
Msa0308770	Msa1174870	0.859137	5.090986e-63	-8.615850e-47
Msa0316750	Msa1174870	0.802193	6.302600e-49	-8.615850e-47
Msa0347610	Msa1174870	0.845035	5.219770e-59	-8.615850e-47
Msa1132890	Msa1174870	0.811431	7.027072e-51	-8.615850e-47
Msa1148720	Msa1174870	0.803429	3.500190e-49	-8.615850e-47
Msa1148750	Msa1174870	0.816050	6.753374e-52	-8.615850e-47
Msa1162980	Msa1174870	0.843813	1.112276e-58	-8.615850e-47
Msa1164530	Msa1174870	0.815651	8.287055e-52	-8.615850e-47
Msa1168040	Msa1174870	-0.850055	2.174030e-60	-8.615850e-47
Msa1174870	Msa1176070	0.811589	6.492973e-51	-8.615850e-47
Msa1174870	Msa1187520	0.803682	3.102779e-49	-8.615850e-47
Msa1174870	Msa1219810	0.846641	1.912059e-59	-8.615850e-47
Msa1174870	Msa1234690	0.981930	8.396746e-154	-8.615850e-47
Msa1174870	Msa1234730	0.991468	8.282860e-188	-8.615850e-47
Msa1174870	Msa1241820	0.802876	4.555365e-49	-8.615850e-47
Msa1174870	Msa1258380	-0.801492	8.776486e-49	-8.615850e-47
Msa1174870	Msa1260130	0.812703	3.710234e-51	-8.615850e-47
Msa1174870	Msa1262920	0.802762	4.810353e-49	-8.615850e-47
Msa1174870	Msa1282920	0.819339	1.223642e-52	-8.615850e-47
Msa1174870	Msa1296880	0.800605	1.332458e-48	-8.615850e-47
Msa1174870	Msa1301650	0.843030	1.800242e-58	-8.615850e-47
Msa1174870	Msa1302100	0.823596	1.271778e-53	-8.615850e-47
Msa1174870	Msa1307740	0.840907	6.552486e-58	-8.615850e-47
Msa1174870	Msa1315110	0.993560	1.373762e-200	-8.615850e-47
Msa1174870	Msa1322190	0.818942	1.505969e-52	-8.615850e-47
Msa1174870	Msa1342100	0.806303	8.784741e-50	-8.615850e-47
Msa1174870	Msa1359630	0.868122	8.252112e-66	-8.615850e-47
Msa1174870	Msa1362840	0.808574	2.896213e-50	-8.615850e-47
Msa1174870	Msa1388860	0.801126	1.043168e-48	-8.615850e-47
Msa1174870	Msa1403340	0.811851	5.691777e-51	-8.615850e-47
Msa1174870	Msa1419970	0.835182	1.947899e-56	-8.615850e-47
Msa1174870	Msa1427830	0.808514	2.982945e-50	-8.615850e-47
Msa1174870	Msa1437910	0.801609	8.305600e-49	-8.615850e-47
Msa1174870	Msa1444250	-0.805237	1.471052e-49	-8.615850e-47
Msa1174870	Msa1459760	-0.817765	2.783195e-52	-8.615850e-47
Msa1174870	Msa1465960	0.823411	1.405008e-53	-8.615850e-47
Msa0489610	Msa1174870	0.828516	8.610400e-55	-8.615850e-47
Msa0504410	Msa1174870	0.861103	1.297404e-63	-8.615850e-47
Msa0504450	Msa1174870	0.811578	6.526955e-51	-8.615850e-47
Msa0516160	Msa1174870	0.820945	5.245471e-53	-8.615850e-47
Msa0519220	Msa1174870	0.818377	2.023719e-52	-8.615850e-47
Msa0532540	Msa1174870	0.816729	4.758129e-52	-8.615850e-47
Msa0536650	Msa1174870	0.803245	3.822982e-49	-8.615850e-47
Msa0540070	Msa1174870	0.807457	5.007607e-50	-8.615850e-47
Msa0561130	Msa1174870	-0.822692	2.067871e-53	-8.615850e-47
Msa0580420	Msa1174870	0.825059	5.760362e-54	-8.615850e-47
Msa0589390	Msa1174870	0.808596	2.866089e-50	-8.615850e-47
Msa0592720	Msa1174870	0.803528	3.339736e-49	-8.615850e-47
Msa0859380	Msa1174870	0.815300	9.921553e-52	-8.615850e-47
Msa0860470	Msa1174870	0.806144	9.487153e-50	-8.615850e-47
Msa0870150	Msa1174870	0.845792	3.255987e-59	-8.615850e-47
Msa0901630	Msa1174870	0.812442	4.230440e-51	-8.615850e-47
Msa0903400	Msa1174870	0.816979	4.182856e-52	-8.615850e-47
Msa0925660	Msa1174870	0.803682	3.101955e-49	-8.615850e-47
Msa0935850	Msa1174870	0.854023	1.622403e-61	-8.615850e-47
Msa0941540	Msa1174870	0.818521	1.877312e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174870	MtrunA17_Chr8g0344721	86.344	227	6	1	1	202	1	227	4.46e-139	389
Msa1174870	MtrunA17_Chr7g0271891	41.176	153	80	5	11	162	17	160	1.83e-30	110
Msa1174870	MtrunA17_Chr4g0044331	44.094	127	64	4	16	138	18	141	1.16e-27	103
Msa1174870	MtrunA17_Chr2g0282761	45.122	82	38	2	10	91	4	78	1.46e-15	74.7
Msa1174870	MtrunA17_Chr7g0263971	48.101	79	36	1	2	80	361	434	2.48e-15	74.7
Msa1174870	MtrunA17_Chr2g0324921	49.275	69	30	1	10	78	4	67	3.97e-15	73.6
Msa1174870	MtrunA17_Chr4g0021141	45.455	77	36	1	3	79	15	85	3.98e-15	70.1
Msa1174870	MtrunA17_Chr4g0061421	53.731	67	29	1	12	78	10	74	1.17e-14	72.8
Msa1174870	MtrunA17_Chr4g0006391	46.377	69	32	1	10	78	4	67	3.73e-14	70.9
Msa1174870	MtrunA17_Chr2g0282741	43.478	69	34	1	10	78	4	67	4.38e-14	70.5
Msa1174870	MtrunA17_Chr4g0040501	43.478	69	34	1	10	78	4	67	6.74e-14	70.1
Msa1174870	MtrunA17_Chr7g0258061	37.273	110	59	3	4	109	16	119	1.97e-13	68.2
Msa1174870	MtrunA17_Chr2g0295831	45.000	80	37	2	9	88	109	181	2.52e-13	67.0
Msa1174870	MtrunA17_Chr5g0427221	38.298	94	43	3	7	96	45	127	3.08e-13	65.1
Msa1174870	MtrunA17_Chr3g0138471	43.478	69	34	1	10	78	4	67	3.60e-13	67.8
Msa1174870	MtrunA17_Chr7g0269841	50.000	68	28	2	11	78	27	88	5.42e-12	64.3
Msa1174870	MtrunA17_Chr8g0378161	44.118	68	34	1	11	78	61	124	6.57e-12	62.4
Msa1174870	MtrunA17_Chr1g0209551	41.558	77	39	1	10	86	16	86	6.63e-12	64.3
Msa1174870	MtrunA17_Chr1g0173931	44.286	70	33	1	9	78	37	100	8.38e-12	63.5
Msa1174870	MtrunA17_Chr5g0399201	42.857	70	37	1	10	79	12	78	9.07e-12	64.3
Msa1174870	MtrunA17_Chr2g0295821	41.250	80	40	2	1	80	95	167	4.48e-11	62.0
Msa1174870	MtrunA17_Chr6g0460381	48.485	66	31	2	10	74	3	66	5.26e-11	60.1
Msa1174870	MtrunA17_Chr3g0123861	44.928	69	31	1	10	78	82	143	5.34e-11	61.6
Msa1174870	MtrunA17_Chr6g0460411	47.143	70	34	2	10	78	2	69	5.71e-11	60.5
Msa1174870	MtrunA17_Chr6g0460461	47.143	70	34	2	10	78	2	69	6.19e-11	60.1
Msa1174870	MtrunA17_Chr5g0415731	38.889	72	42	1	9	78	4	75	6.85e-11	58.5
Msa1174870	MtrunA17_Chr7g0269901	40.625	96	44	4	4	88	90	183	8.07e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174870	AT3G14200.1	50.000	232	93	7	10	234	12	227	9.29e-62	193
Msa1174870	AT1G72416.4	63.810	105	25	2	10	109	5	101	2.00e-40	137
Msa1174870	AT1G72416.2	65.049	103	23	2	10	107	5	99	2.23e-40	137
Msa1174870	AT1G72416.1	65.049	103	23	2	10	107	5	99	3.22e-40	137
Msa1174870	AT1G72416.3	65.049	103	23	2	10	107	5	99	7.72e-40	136
Msa1174870	AT1G71000.1	48.611	144	58	6	4	139	2	137	9.23e-33	117
Msa1174870	AT1G56300.1	49.624	133	58	4	1	129	1	128	1.05e-32	116
Msa1174870	AT1G71000.2	42.593	162	79	5	4	159	2	155	3.69e-28	105
Msa1174870	AT1G56300.2	51.818	110	47	3	1	106	1	108	1.26e-27	102
Msa1174870	AT1G72416.5	73.469	49	13	0	61	109	10	58	1.34e-18	80.1
Msa1174870	AT5G01390.3	49.275	69	30	1	10	78	4	67	3.35e-15	72.8
Msa1174870	AT5G01390.2	49.275	69	30	1	10	78	4	67	6.51e-15	72.8
Msa1174870	AT1G59725.1	46.377	69	33	1	10	78	4	68	9.24e-15	72.8
Msa1174870	AT5G01390.1	49.275	69	30	1	10	78	4	67	1.01e-14	72.8
Msa1174870	AT5G01390.4	49.275	69	30	1	10	78	4	67	1.97e-14	70.5
Msa1174870	AT5G49060.2	46.914	81	35	3	7	86	96	169	3.12e-14	71.2
Msa1174870	AT5G49060.1	46.914	81	35	3	7	86	96	169	3.12e-14	71.2
Msa1174870	AT5G25530.2	44.928	69	33	1	10	78	4	67	5.10e-14	70.9
Msa1174870	AT5G03160.1	46.667	75	35	1	6	80	366	435	5.63e-14	71.2
Msa1174870	AT4G28480.2	46.377	69	32	1	10	78	4	67	7.24e-14	69.7
Msa1174870	AT3G08910.1	46.377	69	32	1	10	78	4	67	7.96e-14	70.1
Msa1174870	AT4G28480.1	46.377	69	32	1	10	78	4	67	1.21e-13	69.7
Msa1174870	AT3G47940.1	42.857	70	36	1	10	79	4	69	2.07e-13	68.9
Msa1174870	AT2G35720.1	42.308	78	42	1	2	79	7	81	2.55e-13	69.3
Msa1174870	AT1G10350.1	42.029	69	35	1	10	78	4	67	3.43e-13	68.6
Msa1174870	AT5G16650.1	39.024	82	43	2	3	83	4	79	6.57e-13	64.3
Msa1174870	AT2G20560.1	44.928	69	33	1	10	78	4	67	6.73e-13	67.4
Msa1174870	AT5G16650.2	39.024	82	43	2	3	83	4	79	2.34e-12	63.5
Msa1174870	AT3G12170.1	38.793	116	61	4	1	113	1	109	2.64e-12	65.1
Msa1174870	AT2G33735.1	50.704	71	25	2	10	78	22	84	3.26e-12	62.0
Msa1174870	AT2G33735.2	50.704	71	25	2	10	78	22	84	3.26e-12	62.0
Msa1174870	AT3G12170.2	37.931	116	62	4	1	113	37	145	5.47e-12	64.7
Msa1174870	AT5G06910.1	37.705	122	59	5	1	116	19	129	6.75e-12	64.3
Msa1174870	AT5G25530.1	41.558	77	32	3	10	78	4	75	1.88e-11	63.2
Msa1174870	AT4G39960.1	40.217	92	42	3	9	96	84	166	2.59e-11	63.2
Msa1174870	AT4G39960.2	40.217	92	42	3	9	96	84	166	2.59e-11	63.2
Msa1174870	AT1G80030.4	42.254	71	34	1	8	78	73	136	4.27e-11	62.4
Msa1174870	AT1G80030.2	42.254	71	34	1	8	78	73	136	5.73e-11	62.4
Msa1174870	AT1G80030.3	42.254	71	34	1	8	78	73	136	5.73e-11	62.4
Msa1174870	AT1G80030.1	42.254	71	34	1	8	78	73	136	5.73e-11	62.4
Msa1174870	AT5G48030.1	31.788	151	88	4	8	151	92	234	8.59e-11	61.6
Msa1174870	AT3G62600.1	48.529	68	29	2	11	78	27	88	8.92e-11	61.2

Find 72 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGAAGTTCTGGTAATT+TGG	0.142853	8_1:+6076395	None:intergenic
AATTCTACTCAGATGAATTA+TGG	0.200136	8_1:-6076446	Msa1174870:CDS
TCTAGTTGGTTGTGAAAATT+TGG	0.205516	8_1:-6079032	Msa1174870:five_prime_UTR
TGTTATTGTTGGAAATAATA+TGG	0.247286	8_1:-6079007	Msa1174870:exon
ATTAGTCATTTGTTGCATTA+TGG	0.247313	8_1:-6075042	Msa1174870:three_prime_UTR
CCTTCTCCACATCTTCTTGA+AGG	0.267807	8_1:+6075493	None:intergenic
GTGTGTCAACCTTAGCATTT+TGG	0.273693	8_1:-6075327	Msa1174870:three_prime_UTR
CAATGATTTCTATGCAGTTT+TGG	0.279803	8_1:-6078965	Msa1174870:CDS
CCAGATCGTTGTTCAGCTTC+AGG	0.283002	8_1:-6078621	Msa1174870:CDS
AATTACCAGAACTTCTGTTT+TGG	0.283009	8_1:-6076392	Msa1174870:CDS
GGCTCACATTTGATTTAGTT+TGG	0.297419	8_1:+6077288	None:intergenic
CAAGCGGATATCGGATTAAA+TGG	0.298600	8_1:-6076551	Msa1174870:CDS
GCAGAGGATTCTTCTAGTTT+TGG	0.305479	8_1:-6076419	Msa1174870:CDS
AGCACCTCTCTTAATGCTTC+GGG	0.329574	8_1:-6076527	Msa1174870:CDS
CTTAAACTCAAACTCCTAGA+TGG	0.329984	8_1:+6075150	None:intergenic
CTTCAACTTGGATGACAACT+TGG	0.330506	8_1:+6079281	None:intergenic
CTAATGTTTCTTTGTATTGT+CGG	0.332006	8_1:+6079103	None:intergenic
TTATATGTGATACAGGAAAA+TGG	0.333099	8_1:-6076620	Msa1174870:intron
TATATAAAGTCTAGAATTAG+AGG	0.347820	8_1:-6079414	Msa1174870:five_prime_UTR
GCAATTCAAGAAGCCTATTC+TGG	0.348966	8_1:-6078558	Msa1174870:intron
AGAAGCTAAGAAGAAATTTC+AGG	0.357982	8_1:-6078580	Msa1174870:CDS
TCATCTACTTTCATGACTTT+CGG	0.362653	8_1:-6076494	Msa1174870:CDS
AAATGCCTTGGAAAATTTGA+TGG	0.373884	8_1:-6075203	Msa1174870:three_prime_UTR
GTTTGGTTGGTTGATCTAGT+TGG	0.390684	8_1:-6079046	Msa1174870:five_prime_UTR
AAGCACCTCTCTTAATGCTT+CGG	0.391365	8_1:-6076528	Msa1174870:CDS
GATATGTTTCAAGCGGATAT+CGG	0.410739	8_1:-6076560	Msa1174870:CDS
TCTGTTGGAGAATTCTAGTT+TGG	0.411390	8_1:-6079063	Msa1174870:five_prime_UTR
ACATTAGTTTGATATTTGAT+TGG	0.420967	8_1:-6079087	Msa1174870:five_prime_UTR
ATTCTACTCAGATGAATTAT+GGG	0.423184	8_1:-6076445	Msa1174870:CDS
GAAGATGTGGAGAAGGAAAA+GGG	0.428449	8_1:-6075486	Msa1174870:CDS
TGACCCCAAAACAGAAGTTC+TGG	0.428777	8_1:+6076387	None:intergenic
AAAATTTGGATTGTTATTGT+TGG	0.435217	8_1:-6079018	Msa1174870:five_prime_UTR
CAGATCGTTGTTCAGCTTCA+GGG	0.447334	8_1:-6078620	Msa1174870:CDS
AAGCAATTGTACACCCATCT+AGG	0.450605	8_1:-6075164	Msa1174870:three_prime_UTR
TGCATGTAAATTACCAGAAT+AGG	0.452286	8_1:+6078545	None:intergenic
AAATGGAGAGGAGAGCTTTG+AGG	0.455188	8_1:-6076603	Msa1174870:CDS
TATCAATTAGCAGAAATTCC+AGG	0.462490	8_1:+6075373	None:intergenic
AGAAGATGTGGAGAAGGAAA+AGG	0.463800	8_1:-6075487	Msa1174870:CDS
CCTGAAGCTGAACAACGATC+TGG	0.468892	8_1:+6078621	None:intergenic
AATTCCAGGATGGTCATTAG+AGG	0.478304	8_1:+6075387	None:intergenic
GGGAGACTTTCTGAACGAAA+TGG	0.482099	8_1:-6077324	Msa1174870:CDS
TTGGATGACAACTTGGGTTG+CGG	0.483218	8_1:+6079288	None:intergenic
TACTAAGATAAGATAAACTA+TGG	0.485649	8_1:+6075247	None:intergenic
GGAAATAATATGGCTAATGA+AGG	0.487826	8_1:-6078997	Msa1174870:exon
TTGGAGAATTCTAGTTTGGT+TGG	0.496008	8_1:-6079059	Msa1174870:five_prime_UTR
TCATGACTTTCGGTGAAAGC+GGG	0.503824	8_1:-6076484	Msa1174870:CDS
TTCCCATGACCAAAATGCTA+AGG	0.508517	8_1:+6075318	None:intergenic
TGATATTTGATTGGTTCTGT+TGG	0.511150	8_1:-6079078	Msa1174870:five_prime_UTR
AATGCTCTGATTCAGAACTA+AGG	0.513112	8_1:-6078930	Msa1174870:CDS
TTCAGGGAATGTGAAGTTTG+TGG	0.516942	8_1:-6078604	Msa1174870:CDS
ATGGGTGTACAATTGCTTGA+AGG	0.522891	8_1:+6075169	None:intergenic
TTAGGAGGTCACAAATGCCT+TGG	0.526911	8_1:-6075215	Msa1174870:three_prime_UTR
AGTCATGAAAGTAGATGAAG+TGG	0.538936	8_1:+6076499	None:intergenic
AAGATGTGGAGAAGGAAAAG+GGG	0.541100	8_1:-6075485	Msa1174870:CDS
TGCTTATAAGAAACTTGCAC+TGG	0.543386	8_1:-6078905	Msa1174870:intron
TTCAACTTGGATGACAACTT+GGG	0.545053	8_1:+6079282	None:intergenic
ATTAATATACAACTTCAACT+TGG	0.554791	8_1:+6079269	None:intergenic
TTAAACTCAAACTCCTAGAT+GGG	0.559329	8_1:+6075151	None:intergenic
GTTTCCTCTAATGACCATCC+TGG	0.561659	8_1:-6075391	Msa1174870:CDS
ATTTGATGATATGTTTCAAG+CGG	0.571158	8_1:-6076567	Msa1174870:CDS
TTTAGACTAGATGGAATGAA+GGG	0.573998	8_1:+6079352	None:intergenic
TTCATGACTTTCGGTGAAAG+CGG	0.579461	8_1:-6076485	Msa1174870:CDS
AGATGTGGAGAAGGAAAAGG+GGG	0.580651	8_1:-6075484	Msa1174870:CDS
CCTTCAAGAAGATGTGGAGA+AGG	0.586502	8_1:-6075493	Msa1174870:CDS
GAAGCACCTTCAAGAAGATG+TGG	0.587778	8_1:-6075499	Msa1174870:CDS
GATGAATTATGGGAAGGCAG+AGG	0.603296	8_1:-6076435	Msa1174870:CDS
TACTCAGATGAATTATGGGA+AGG	0.609702	8_1:-6076441	Msa1174870:CDS
TGTGATACAGGAAAATGGAG+AGG	0.651222	8_1:-6076615	Msa1174870:intron
AAGAGATTAATGTACGACGT+TGG	0.670458	8_1:-6078377	Msa1174870:CDS
AATTAGCAGAAATTCCAGGA+TGG	0.683865	8_1:+6075377	None:intergenic
GCAACCCGAAGCATTAAGAG+AGG	0.689028	8_1:+6076523	None:intergenic
AGGTCTCTAGAGTATTCATG+AGG	0.690314	8_1:-6079394	Msa1174870:five_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATTACTTGTTTTTATATAT+TGG	-	chr8_1:6075978-6075997	Msa1174870:intron	10.0%
!!!	ATTACTTGTTTTTATATATT+GGG	-	chr8_1:6075979-6075998	Msa1174870:intron	10.0%
!!	TTGATTATATGAAAAAAAAT+CGG	+	chr8_1:6076696-6076715	None:intergenic	10.0%
!!	TATTGCAAAAATATTAAATA+GGG	+	chr8_1:6077166-6077185	None:intergenic	10.0%
!!	ATATTGCAAAAATATTAAAT+AGG	+	chr8_1:6077167-6077186	None:intergenic	10.0%
!!!	TTTATATATTTTTTGTTTAG+TGG	-	chr8_1:6077263-6077282	Msa1174870:intron	10.0%
!!	TATTTAAAAAATAATTCAAG+TGG	-	chr8_1:6077503-6077522	Msa1174870:intron	10.0%
!!!	TGAATAAACTTTAGAAAAAA+CGG	+	chr8_1:6075623-6075642	None:intergenic	15.0%
!!	AGGAAAATAATTTGATTAAA+TGG	-	chr8_1:6075773-6075792	Msa1174870:intron	15.0%
!!!	TTCTCAATCTATTTTTTAAA+CGG	+	chr8_1:6076401-6076420	None:intergenic	15.0%
!!	TGATTATATGAAAAAAAATC+GGG	+	chr8_1:6076695-6076714	None:intergenic	15.0%
!!!	AACAAGATATTATTTTATTG+TGG	-	chr8_1:6077544-6077563	Msa1174870:intron	15.0%
!!	AACTTGAATTAATCAATTTA+TGG	+	chr8_1:6077697-6077716	None:intergenic	15.0%
!!!	CTTTAAATTATACAAGATTT+TGG	-	chr8_1:6078265-6078284	Msa1174870:intron	15.0%
!!!	TATATAAAGTCTAGAATTAG+AGG	-	chr8_1:6075016-6075035	Msa1174870:three_prime_UTR	20.0%
!!	ATTAATATACAACTTCAACT+TGG	+	chr8_1:6075164-6075183	None:intergenic	20.0%
!!!	GTTTCTTACTTTTTATGTTA+AGG	-	chr8_1:6075259-6075278	Msa1174870:three_prime_UTR	20.0%
!!!	ACGAATTTTGTGTTTTTATT+TGG	-	chr8_1:6075286-6075305	Msa1174870:three_prime_UTR	20.0%
!!!	TTTTGTGTTTTTATTTGGTT+TGG	-	chr8_1:6075291-6075310	Msa1174870:three_prime_UTR	20.0%
!!!	ACATTAGTTTGATATTTGAT+TGG	-	chr8_1:6075343-6075362	Msa1174870:three_prime_UTR	20.0%
!!!	AAAATTTGGATTGTTATTGT+TGG	-	chr8_1:6075412-6075431	Msa1174870:CDS	20.0%
!!	TGTTATTGTTGGAAATAATA+TGG	-	chr8_1:6075423-6075442	Msa1174870:CDS	20.0%
!!!	TTTTCTAAAGTTTATTCACA+TGG	-	chr8_1:6075625-6075644	Msa1174870:intron	20.0%
!!!	TTTCTAAAGTTTATTCACAT+GGG	-	chr8_1:6075626-6075645	Msa1174870:intron	20.0%
!!	TATCTTTCTTTCACAAATTT+AGG	+	chr8_1:6075703-6075722	None:intergenic	20.0%
!!!	TTTGTGAAAGAAAGATATTT+TGG	-	chr8_1:6075706-6075725	Msa1174870:intron	20.0%
!!	ACAAGTAATAATTTGAATCA+TGG	+	chr8_1:6075968-6075987	None:intergenic	20.0%
!!!	ATGTAAATGGTGTTTTTTTT+TGG	-	chr8_1:6076008-6076027	Msa1174870:intron	20.0%
!!!	TGTAAATGGTGTTTTTTTTT+GGG	-	chr8_1:6076009-6076028	Msa1174870:intron	20.0%
!!	AAAGTGAAAAGGTAAAATTA+AGG	+	chr8_1:6076228-6076247	None:intergenic	20.0%
!!	ATTCAGTTTCAATATTCATT+AGG	+	chr8_1:6076280-6076299	None:intergenic	20.0%
!!	ATGAATATTGAAACTGAATT+AGG	-	chr8_1:6076281-6076300	Msa1174870:intron	20.0%
!!	TGAATATTGAAACTGAATTA+GGG	-	chr8_1:6076282-6076301	Msa1174870:intron	20.0%
!!!	TGGAAATGAGTTAATTTTTT+AGG	-	chr8_1:6076501-6076520	Msa1174870:CDS	20.0%
!!!	GTTTAAAATAGTGACTTAAT+TGG	+	chr8_1:6076573-6076592	None:intergenic	20.0%
!!	TTATAGCACATCAAAATTAT+TGG	+	chr8_1:6077054-6077073	None:intergenic	20.0%
!!	AACAAGACATAAACATTATT+AGG	+	chr8_1:6077365-6077384	None:intergenic	20.0%
!!!	AATAATGTTTATGTCTTGTT+TGG	-	chr8_1:6077365-6077384	Msa1174870:intron	20.0%
!!	CAAGATTTGAAAATAATTGT+TGG	-	chr8_1:6077578-6077597	Msa1174870:intron	20.0%
!!	ATTTGAAAATAATTGTTGGA+AGG	-	chr8_1:6077582-6077601	Msa1174870:intron	20.0%
!!!	AGTTAAAAGGATTGAAATTT+TGG	+	chr8_1:6077749-6077768	None:intergenic	20.0%
!!	AATGAATTGAAAGAGTTAAA+AGG	+	chr8_1:6077762-6077781	None:intergenic	20.0%
!!	GATTAATTTATATGTGATAC+AGG	-	chr8_1:6077803-6077822	Msa1174870:intron	20.0%
!!	TTTAACATGTAATTGAGTTA+TGG	-	chr8_1:6078236-6078255	Msa1174870:intron	20.0%
!!	TTAACATGTAATTGAGTTAT+GGG	-	chr8_1:6078237-6078256	Msa1174870:intron	20.0%
!!!	ATGATAGAAGATTTAGAATT+TGG	-	chr8_1:6078298-6078317	Msa1174870:intron	20.0%
!!	ACTCTATTAATAACTCAAAA+TGG	+	chr8_1:6078740-6078759	None:intergenic	20.0%
!!	TTTGTATCAAATTCTCAAAT+TGG	+	chr8_1:6078769-6078788	None:intergenic	20.0%
!!!	TGCAATTTTTTTAAGAAGAT+TGG	-	chr8_1:6078847-6078866	Msa1174870:intron	20.0%
!!!	TCCAACATTTTTTTTATTCT+TGG	-	chr8_1:6078895-6078914	Msa1174870:intron	20.0%
!!	ACCAAGAATAAAAAAAATGT+TGG	+	chr8_1:6078899-6078918	None:intergenic	20.0%
!!	ATGTACAACTAAAAAGATTA+GGG	-	chr8_1:6079154-6079173	Msa1174870:five_prime_UTR	20.0%
!!	TACTAAGATAAGATAAACTA+TGG	+	chr8_1:6079186-6079205	None:intergenic	20.0%
!!	ATCTTAGTACTAAAAAGATT+AGG	-	chr8_1:6079197-6079216	Msa1174870:five_prime_UTR	20.0%
!!!	AATGTCAATTTTGATTTGAA+AGG	-	chr8_1:6079421-6079440	Msa1174870:five_prime_UTR	20.0%
!	CTAATGTTTCTTTGTATTGT+CGG	+	chr8_1:6075330-6075349	None:intergenic	25.0%
!!	AATGATTTCTATGCAGTTTT+GGG	-	chr8_1:6075466-6075485	Msa1174870:CDS	25.0%
!!	ATAAACTTTAGAAAAAACGG+TGG	+	chr8_1:6075620-6075639	None:intergenic	25.0%
!!	ATTGTTGTTATGATGAAAGT+TGG	-	chr8_1:6075738-6075757	Msa1174870:intron	25.0%
!	AAAGTTGGAATTGTTTAGAA+AGG	-	chr8_1:6075753-6075772	Msa1174870:intron	25.0%
!	TTTGATTAAATGGACAGAAA+TGG	-	chr8_1:6075783-6075802	Msa1174870:intron	25.0%
!!	TATTGGGTCTAAAATGTAAA+TGG	-	chr8_1:6075995-6076014	Msa1174870:intron	25.0%
!	CATAACACAAAAATAGAAGT+AGG	+	chr8_1:6076163-6076182	None:intergenic	25.0%
!!	TTGTTTCTTCATTTACTTGA+AGG	-	chr8_1:6076185-6076204	Msa1174870:intron	25.0%
!	TGTTTCTTCATTTACTTGAA+GGG	-	chr8_1:6076186-6076205	Msa1174870:intron	25.0%
!	TTGTTAATGTGAAAGTGAAA+AGG	+	chr8_1:6076239-6076258	None:intergenic	25.0%
!!	CACTTTCACATTAACAATTT+TGG	-	chr8_1:6076242-6076261	Msa1174870:intron	25.0%
!!	TATAAGGATGATTTGATAGT+TGG	-	chr8_1:6076306-6076325	Msa1174870:intron	25.0%
!	AACAATGATATGGTCAATTT+CGG	-	chr8_1:6076456-6076475	Msa1174870:CDS	25.0%
!	CAACGAAAACAAGAAAAAAA+TGG	-	chr8_1:6076481-6076500	Msa1174870:CDS	25.0%
!!!	AGTCACTATTTTAAACCTAA+AGG	-	chr8_1:6076577-6076596	Msa1174870:CDS	25.0%
!	AAGAGGAGAAGAAAAATAAT+TGG	+	chr8_1:6076799-6076818	None:intergenic	25.0%
!	AAAAAGAGATATGTTCTTCA+AGG	-	chr8_1:6076827-6076846	Msa1174870:intron	25.0%
!!!	ACATTTTTTTTTCCTCCTTT+GGG	+	chr8_1:6076889-6076908	None:intergenic	25.0%
!!!	AACATTTTTTTTTCCTCCTT+TGG	+	chr8_1:6076890-6076909	None:intergenic	25.0%
!	GATTCAATTCCATGAATTAT+TGG	+	chr8_1:6077010-6077029	None:intergenic	25.0%
!	ATTCATGGAATTGAATCAAA+TGG	-	chr8_1:6077013-6077032	Msa1174870:intron	25.0%
!!!	AAACTGAATTTTGTTTTGCA+GGG	-	chr8_1:6077080-6077099	Msa1174870:intron	25.0%
!!!	TTTTGTTTGCTTGTAATTAG+TGG	-	chr8_1:6077194-6077213	Msa1174870:intron	25.0%
!!	AATCAATTTATGGAAAGTAC+AGG	+	chr8_1:6077687-6077706	None:intergenic	25.0%
!	TTATATGTGATACAGGAAAA+TGG	-	chr8_1:6077810-6077829	Msa1174870:intron	25.0%
!!	ATTTGATGATATGTTTCAAG+CGG	-	chr8_1:6077863-6077882	Msa1174870:intron	25.0%
!	AATTCTACTCAGATGAATTA+TGG	-	chr8_1:6077984-6078003	Msa1174870:intron	25.0%
!	ATTCTACTCAGATGAATTAT+GGG	-	chr8_1:6077985-6078004	Msa1174870:intron	25.0%
!	TTGAATGATTGTTTATTGTG+AGG	-	chr8_1:6078161-6078180	Msa1174870:intron	25.0%
!	AGTAGTAACAACAAAAAGAT+AGG	-	chr8_1:6078213-6078232	Msa1174870:intron	25.0%
!	TAACATGTAATTGAGTTATG+GGG	-	chr8_1:6078238-6078257	Msa1174870:intron	25.0%
!	TAATCTATCTTCAGAGTAAT+TGG	+	chr8_1:6078466-6078485	None:intergenic	25.0%
!	TTAAGTGGTTAAGGAAAAAA+TGG	-	chr8_1:6078483-6078502	Msa1174870:intron	25.0%
!	CACTAATTAAAAGACTCATT+GGG	+	chr8_1:6078531-6078550	None:intergenic	25.0%
!!!	TTTTGTTGTTCATGTTATCA+AGG	-	chr8_1:6078558-6078577	Msa1174870:intron	25.0%
!	ATTATCATTACCAATGAATG+AGG	-	chr8_1:6078593-6078612	Msa1174870:CDS	25.0%
!	TTACAAAACTAATAGAGTCA+GGG	-	chr8_1:6078681-6078700	Msa1174870:intron	25.0%
!	AGAAAACAAAAAATCTCATG+TGG	-	chr8_1:6079009-6079028	Msa1174870:exon	25.0%
!	CATGTACAACTAAAAAGATT+AGG	-	chr8_1:6079153-6079172	Msa1174870:five_prime_UTR	25.0%
!	TGTACAACTAAAAAGATTAG+GGG	-	chr8_1:6079155-6079174	Msa1174870:five_prime_UTR	25.0%
!	TTAGTACTAAAAAGATTAGG+AGG	-	chr8_1:6079200-6079219	Msa1174870:five_prime_UTR	25.0%
!!	ATTAGTCATTTGTTGCATTA+TGG	-	chr8_1:6079388-6079407	Msa1174870:five_prime_UTR	25.0%
	TTTAGACTAGATGGAATGAA+GGG	+	chr8_1:6075081-6075100	None:intergenic	30.0%
!	TTTTAGACTAGATGGAATGA+AGG	+	chr8_1:6075082-6075101	None:intergenic	30.0%
!!!	GCATTTTGTTTTAGACTAGA+TGG	+	chr8_1:6075090-6075109	None:intergenic	30.0%
!!	TGATATTTGATTGGTTCTGT+TGG	-	chr8_1:6075352-6075371	Msa1174870:three_prime_UTR	30.0%
	TCTAGTTGGTTGTGAAAATT+TGG	-	chr8_1:6075398-6075417	Msa1174870:CDS	30.0%
	GGAAATAATATGGCTAATGA+AGG	-	chr8_1:6075433-6075452	Msa1174870:CDS	30.0%
!	CAATGATTTCTATGCAGTTT+TGG	-	chr8_1:6075465-6075484	Msa1174870:CDS	30.0%
	AGAAGCTAAGAAGAAATTTC+AGG	-	chr8_1:6075850-6075869	Msa1174870:intron	30.0%
	TGCATGTAAATTACCAGAAT+AGG	+	chr8_1:6075888-6075907	None:intergenic	30.0%
	TAATTTGAATCATGGCTCAT+GGG	+	chr8_1:6075960-6075979	None:intergenic	30.0%
	ATAATTTGAATCATGGCTCA+TGG	+	chr8_1:6075961-6075980	None:intergenic	30.0%
	GTTTCAATATTCATTAGGCA+TGG	+	chr8_1:6076275-6076294	None:intergenic	30.0%
	GAAACTGAATTAGGGATATA+AGG	-	chr8_1:6076290-6076309	Msa1174870:intron	30.0%
	TCACTGTCATATTTACCTTT+AGG	+	chr8_1:6076595-6076614	None:intergenic	30.0%
	GGAGAAGAAAAATAATTGGA+CGG	+	chr8_1:6076795-6076814	None:intergenic	30.0%
	ATATGTTCTTCAAGGTTAGT+AGG	-	chr8_1:6076835-6076854	Msa1174870:intron	30.0%
	TTTGGGAAAAGACATTTGTT+GGG	+	chr8_1:6076872-6076891	None:intergenic	30.0%
	CAATAAGAGCCAATAATTCA+TGG	-	chr8_1:6076998-6077017	Msa1174870:intron	30.0%
!!!	GAAACTGAATTTTGTTTTGC+AGG	-	chr8_1:6077079-6077098	Msa1174870:intron	30.0%
!!!	AATTTTGTTTTGCAGGGTAT+GGG	-	chr8_1:6077086-6077105	Msa1174870:intron	30.0%
	TAATTGTTGGAAGGATAAAG+TGG	-	chr8_1:6077591-6077610	Msa1174870:intron	30.0%
	TCATCTACTTTCATGACTTT+CGG	-	chr8_1:6077936-6077955	Msa1174870:intron	30.0%
!	AAACAGAAGTTCTGGTAATT+TGG	+	chr8_1:6078038-6078057	None:intergenic	30.0%
!	AATTACCAGAACTTCTGTTT+TGG	-	chr8_1:6078038-6078057	Msa1174870:intron	30.0%
!	ATTACCAGAACTTCTGTTTT+GGG	-	chr8_1:6078039-6078058	Msa1174870:intron	30.0%
	ATGAATGTTGCATCAAATGA+AGG	+	chr8_1:6078099-6078118	None:intergenic	30.0%
	TTCATTTGATGCAACATTCA+TGG	-	chr8_1:6078098-6078117	Msa1174870:intron	30.0%
	ATAACAATTCATGCTTCAGA+TGG	+	chr8_1:6078425-6078444	None:intergenic	30.0%
!	TACTCTGAAGATAGATTAAG+TGG	-	chr8_1:6078468-6078487	Msa1174870:intron	30.0%
	GAAGATAGATTAAGTGGTTA+AGG	-	chr8_1:6078474-6078493	Msa1174870:intron	30.0%
	CCACTAATTAAAAGACTCAT+TGG	+	chr8_1:6078532-6078551	None:intergenic	30.0%
!!	CCAATGAGTCTTTTAATTAG+TGG	-	chr8_1:6078529-6078548	Msa1174870:intron	30.0%
!	TGTTTTGTATCCTCATTCAT+TGG	+	chr8_1:6078606-6078625	None:intergenic	30.0%
	TGAATGAGGATACAAAACAA+TGG	-	chr8_1:6078607-6078626	Msa1174870:CDS	30.0%
!!!	CTGACTCTATTAGTTTTGTA+AGG	+	chr8_1:6078682-6078701	None:intergenic	30.0%
	CTTACAAAACTAATAGAGTC+AGG	-	chr8_1:6078680-6078699	Msa1174870:intron	30.0%
	AAAGACAGAGAGTAATTTGA+TGG	-	chr8_1:6078786-6078805	Msa1174870:intron	30.0%
	CACTCCATATATTATATAGC+TGG	-	chr8_1:6078811-6078830	Msa1174870:intron	30.0%
	GAGACCAGCTATATAATATA+TGG	+	chr8_1:6078818-6078837	None:intergenic	30.0%
	CAGTATCAAGTTAAATGTAG+TGG	+	chr8_1:6078874-6078893	None:intergenic	30.0%
	GGTAGCAAAAAACAAAGAAA+TGG	-	chr8_1:6078985-6079004	Msa1174870:CDS	30.0%
	TATCAATTAGCAGAAATTCC+AGG	+	chr8_1:6079060-6079079	None:intergenic	30.0%
	GTACAACTAAAAAGATTAGG+GGG	-	chr8_1:6079156-6079175	Msa1174870:five_prime_UTR	30.0%
	AAATGCCTTGGAAAATTTGA+TGG	-	chr8_1:6079227-6079246	Msa1174870:five_prime_UTR	30.0%
	TTAAACTCAAACTCCTAGAT+GGG	+	chr8_1:6079282-6079301	None:intergenic	30.0%
	TTCAACTTGGATGACAACTT+GGG	+	chr8_1:6075151-6075170	None:intergenic	35.0%
!	TCTGTTGGAGAATTCTAGTT+TGG	-	chr8_1:6075367-6075386	Msa1174870:exon	35.0%
!!	TTGGAGAATTCTAGTTTGGT+TGG	-	chr8_1:6075371-6075390	Msa1174870:exon	35.0%
!!!	TGCAGTTTTGGGATTGAATA+AGG	-	chr8_1:6075477-6075496	Msa1174870:CDS	35.0%
	AATGCTCTGATTCAGAACTA+AGG	-	chr8_1:6075500-6075519	Msa1174870:CDS	35.0%
	TGCTTATAAGAAACTTGCAC+TGG	-	chr8_1:6075525-6075544	Msa1174870:intron	35.0%
	GGTTAGAGTAGAAACAAAGA+CGG	+	chr8_1:6075592-6075611	None:intergenic	35.0%
!	ACTTTAGAAAAAACGGTGGA+GGG	+	chr8_1:6075616-6075635	None:intergenic	35.0%
!	AACTTTAGAAAAAACGGTGG+AGG	+	chr8_1:6075617-6075636	None:intergenic	35.0%
	AAGAGATTAATGTACGACGT+TGG	-	chr8_1:6076053-6076072	Msa1174870:intron	35.0%
	ATTTCACACCATTCATGAGT+TGG	-	chr8_1:6076115-6076134	Msa1174870:intron	35.0%
	TTTCACACCATTCATGAGTT+GGG	-	chr8_1:6076116-6076135	Msa1174870:intron	35.0%
!	AAGGATGATTTGATAGTTGG+TGG	-	chr8_1:6076309-6076328	Msa1174870:intron	35.0%
!	AGGATGATTTGATAGTTGGT+GGG	-	chr8_1:6076310-6076329	Msa1174870:intron	35.0%
	TTAAAGATCAGAGTGATGAG+CGG	-	chr8_1:6076337-6076356	Msa1174870:intron	35.0%
	TGAGAATGAATTTCCTCTCT+TGG	-	chr8_1:6076415-6076434	Msa1174870:CDS	35.0%
	TTGACCATATCATTGTTTCG+TGG	+	chr8_1:6076453-6076472	None:intergenic	35.0%
!!!	TCTCTTTTTTCACGTGAAAG+AGG	+	chr8_1:6076816-6076835	None:intergenic	35.0%
!	TTGGGAAAAGACATTTGTTG+GGG	+	chr8_1:6076871-6076890	None:intergenic	35.0%
!	CTTTGGGAAAAGACATTTGT+TGG	+	chr8_1:6076873-6076892	None:intergenic	35.0%
!	CAACAAATGTCTTTTCCCAA+AGG	-	chr8_1:6076871-6076890	Msa1174870:intron	35.0%
	TATGTGTCACATAACACATG+TGG	-	chr8_1:6076950-6076969	Msa1174870:intron	35.0%
	CATGGAATTGAATCAAATGG+AGG	-	chr8_1:6077016-6077035	Msa1174870:intron	35.0%
!!!	GAATTTTGTTTTGCAGGGTA+TGG	-	chr8_1:6077085-6077104	Msa1174870:intron	35.0%
!	GGCTCACATTTGATTTAGTT+TGG	+	chr8_1:6077145-6077164	None:intergenic	35.0%
	CTTGTAATTAGTGGAACGTT+AGG	-	chr8_1:6077203-6077222	Msa1174870:intron	35.0%
	AATTAGTGGAACGTTAGGTT+AGG	-	chr8_1:6077208-6077227	Msa1174870:intron	35.0%
	AGGCACAACTACATAGTATT+AGG	+	chr8_1:6077321-6077340	None:intergenic	35.0%
!	AGTTGTGCCTTTTTACCTTT+TGG	-	chr8_1:6077331-6077350	Msa1174870:CDS	35.0%
!	GTTGTGCCTTTTTACCTTTT+GGG	-	chr8_1:6077332-6077351	Msa1174870:CDS	35.0%
!!!	CCTTTTTACCTTTTGGGATT+GGG	-	chr8_1:6077338-6077357	Msa1174870:intron	35.0%
	ACACCAACTATCATAGCAAT+TGG	+	chr8_1:6077392-6077411	None:intergenic	35.0%
!	AATTGCTATGATAGTTGGTG+TGG	-	chr8_1:6077391-6077410	Msa1174870:intron	35.0%
	GATATGTTTCAAGCGGATAT+CGG	-	chr8_1:6077870-6077889	Msa1174870:intron	35.0%
	AGTCATGAAAGTAGATGAAG+TGG	+	chr8_1:6077934-6077953	None:intergenic	35.0%
	TACTCAGATGAATTATGGGA+AGG	-	chr8_1:6077989-6078008	Msa1174870:intron	35.0%
!	TTACCAGAACTTCTGTTTTG+GGG	-	chr8_1:6078040-6078059	Msa1174870:intron	35.0%
	GGCAAACATATAGCATAGTA+AGG	-	chr8_1:6078119-6078138	Msa1174870:intron	35.0%
	TGTTACTACTTACTACCCAT+AGG	+	chr8_1:6078203-6078222	None:intergenic	35.0%
	TATCTTCAGAGTAATTGGCA+AGG	+	chr8_1:6078461-6078480	None:intergenic	35.0%
	ACAAAACAATGGAGTATGTG+TGG	-	chr8_1:6078618-6078637	Msa1174870:CDS	35.0%
	ATGTGTGGAGTATATAGAAG+TGG	-	chr8_1:6078633-6078652	Msa1174870:CDS	35.0%
!!	AGTTTTGTAAGGAGATCACT+AGG	+	chr8_1:6078671-6078690	None:intergenic	35.0%
	TTGTCAAACGTTTCTCAGAT+TGG	+	chr8_1:6078708-6078727	None:intergenic	35.0%
	AATTAGCAGAAATTCCAGGA+TGG	+	chr8_1:6079056-6079075	None:intergenic	35.0%
!!	AACCTTAGCATTTTGGTCAT+GGG	-	chr8_1:6079110-6079129	Msa1174870:five_prime_UTR	35.0%
!	CACTACCATCAAATTTTCCA+AGG	+	chr8_1:6079235-6079254	None:intergenic	35.0%
	CTTAAACTCAAACTCCTAGA+TGG	+	chr8_1:6079283-6079302	None:intergenic	35.0%
!!	AGGTCTCTAGAGTATTCATG+AGG	-	chr8_1:6075036-6075055	Msa1174870:three_prime_UTR	40.0%
	CTTCAACTTGGATGACAACT+TGG	+	chr8_1:6075152-6075171	None:intergenic	40.0%
!	GTTTGGTTGGTTGATCTAGT+TGG	-	chr8_1:6075384-6075403	Msa1174870:CDS	40.0%
	TTCAGGGAATGTGAAGTTTG+TGG	-	chr8_1:6075826-6075845	Msa1174870:intron	40.0%
	GCAATTCAAGAAGCCTATTC+TGG	-	chr8_1:6075872-6075891	Msa1174870:intron	40.0%
	AGGCAAGTGAAAGAGAAAGA+GGG	+	chr8_1:6076143-6076162	None:intergenic	40.0%
	TAGGCAAGTGAAAGAGAAAG+AGG	+	chr8_1:6076144-6076163	None:intergenic	40.0%
!	GGATGATTTGATAGTTGGTG+GGG	-	chr8_1:6076311-6076330	Msa1174870:intron	40.0%
	TAAAGATCAGAGTGATGAGC+GGG	-	chr8_1:6076338-6076357	Msa1174870:intron	40.0%
	GAAGCAAATATGACCAAGAG+AGG	+	chr8_1:6076431-6076450	None:intergenic	40.0%
	GCTTCCACGAAACAATGATA+TGG	-	chr8_1:6076446-6076465	Msa1174870:CDS	40.0%
!	CAAATGTCTTTTCCCAAAGG+AGG	-	chr8_1:6076874-6076893	Msa1174870:intron	40.0%
!!!	GCCTTTTTACCTTTTGGGAT+TGG	-	chr8_1:6077337-6077356	Msa1174870:intron	40.0%
	CCCAATCCCAAAAGGTAAAA+AGG	+	chr8_1:6077341-6077360	None:intergenic	40.0%
	TATTAGGACCCAATCCCAAA+AGG	+	chr8_1:6077349-6077368	None:intergenic	40.0%
	GGTCCAATTGCTATGATAGT+TGG	-	chr8_1:6077386-6077405	Msa1174870:intron	40.0%
	GGAAGGATAAAGTGGTTTAC+TGG	-	chr8_1:6077599-6077618	Msa1174870:intron	40.0%
	GATAAAGTGGTTTACTGGAC+CGG	-	chr8_1:6077604-6077623	Msa1174870:intron	40.0%
	ATAAAGTGGTTTACTGGACC+GGG	-	chr8_1:6077605-6077624	Msa1174870:intron	40.0%
	TGTGATACAGGAAAATGGAG+AGG	-	chr8_1:6077815-6077834	Msa1174870:intron	40.0%
	CAAGCGGATATCGGATTAAA+TGG	-	chr8_1:6077879-6077898	Msa1174870:intron	40.0%
	AAGCACCTCTCTTAATGCTT+CGG	-	chr8_1:6077902-6077921	Msa1174870:intron	40.0%
	TTCATGACTTTCGGTGAAAG+CGG	-	chr8_1:6077945-6077964	Msa1174870:intron	40.0%
!!!	GCAGAGGATTCTTCTAGTTT+TGG	-	chr8_1:6078011-6078030	Msa1174870:intron	40.0%
	TGTGAGGTCCTTTACTTGTT+TGG	-	chr8_1:6078177-6078196	Msa1174870:intron	40.0%
	TCCTTTACTTGTTTGGCCTA+TGG	-	chr8_1:6078184-6078203	Msa1174870:intron	40.0%
	CCTTTACTTGTTTGGCCTAT+GGG	-	chr8_1:6078185-6078204	Msa1174870:intron	40.0%
	GATGCAACTATTGTAGTCGT+TGG	-	chr8_1:6078342-6078361	Msa1174870:intron	40.0%
!	AATGCACATTTGTTGGATCC+TGG	+	chr8_1:6078372-6078391	None:intergenic	40.0%
	TGCAACGAATGCACATTTGT+TGG	+	chr8_1:6078379-6078398	None:intergenic	40.0%
	AGAAGATGTGGAGAAGGAAA+AGG	-	chr8_1:6078943-6078962	Msa1174870:CDS	40.0%
	GAAGATGTGGAGAAGGAAAA+GGG	-	chr8_1:6078944-6078963	Msa1174870:CDS	40.0%
	AAGATGTGGAGAAGGAAAAG+GGG	-	chr8_1:6078945-6078964	Msa1174870:CDS	40.0%
	AATTCCAGGATGGTCATTAG+AGG	+	chr8_1:6079046-6079065	None:intergenic	40.0%
!	GTGTGTCAACCTTAGCATTT+TGG	-	chr8_1:6079103-6079122	Msa1174870:five_prime_UTR	40.0%
!!	CAACCTTAGCATTTTGGTCA+TGG	-	chr8_1:6079109-6079128	Msa1174870:five_prime_UTR	40.0%
	TTCCCATGACCAAAATGCTA+AGG	+	chr8_1:6079115-6079134	None:intergenic	40.0%
	ATGGGTGTACAATTGCTTGA+AGG	+	chr8_1:6079264-6079283	None:intergenic	40.0%
	AAGCAATTGTACACCCATCT+AGG	-	chr8_1:6079266-6079285	Msa1174870:five_prime_UTR	40.0%
!	TTGGATGACAACTTGGGTTG+CGG	+	chr8_1:6075145-6075164	None:intergenic	45.0%
	TTAGAAAAAACGGTGGAGGG+TGG	+	chr8_1:6075613-6075632	None:intergenic	45.0%
	CAGATCGTTGTTCAGCTTCA+GGG	-	chr8_1:6075810-6075829	Msa1174870:intron	45.0%
	TCACATAACACATGTGGCAG+TGG	-	chr8_1:6076956-6076975	Msa1174870:intron	45.0%
	GGGAGACTTTCTGAACGAAA+TGG	-	chr8_1:6077106-6077125	Msa1174870:intron	45.0%
!	TGCTATGATAGTTGGTGTGG+TGG	-	chr8_1:6077394-6077413	Msa1174870:intron	45.0%
!	GCTATGATAGTTGGTGTGGT+GGG	-	chr8_1:6077395-6077414	Msa1174870:intron	45.0%
!	CTATGATAGTTGGTGTGGTG+GGG	-	chr8_1:6077396-6077415	Msa1174870:intron	45.0%
!	TATGATAGTTGGTGTGGTGG+GGG	-	chr8_1:6077397-6077416	Msa1174870:intron	45.0%
!	TGTGGTGGGGGTATATTGTT+GGG	-	chr8_1:6077409-6077428	Msa1174870:intron	45.0%
	TATTGTACGAACACACAGCC+CGG	+	chr8_1:6077626-6077645	None:intergenic	45.0%
	AAATGGAGAGGAGAGCTTTG+AGG	-	chr8_1:6077827-6077846	Msa1174870:intron	45.0%
	AGCACCTCTCTTAATGCTTC+GGG	-	chr8_1:6077903-6077922	Msa1174870:intron	45.0%
	TCATGACTTTCGGTGAAAGC+GGG	-	chr8_1:6077946-6077965	Msa1174870:intron	45.0%
	GATGAATTATGGGAAGGCAG+AGG	-	chr8_1:6077995-6078014	Msa1174870:intron	45.0%
	TGACCCCAAAACAGAAGTTC+TGG	+	chr8_1:6078046-6078065	None:intergenic	45.0%
	CCCATAGGCCAAACAAGTAA+AGG	+	chr8_1:6078188-6078207	None:intergenic	45.0%
!	GAAGCACCTTCAAGAAGATG+TGG	-	chr8_1:6078931-6078950	Msa1174870:CDS	45.0%
	CCTTCTCCACATCTTCTTGA+AGG	+	chr8_1:6078940-6078959	None:intergenic	45.0%
	CCTTCAAGAAGATGTGGAGA+AGG	-	chr8_1:6078937-6078956	Msa1174870:CDS	45.0%
	AGATGTGGAGAAGGAAAAGG+GGG	-	chr8_1:6078946-6078965	Msa1174870:CDS	45.0%
	GGAAAAGGGGGAATTCTAGA+AGG	-	chr8_1:6078958-6078977	Msa1174870:CDS	45.0%
!	AAAGGGGGAATTCTAGAAGG+AGG	-	chr8_1:6078961-6078980	Msa1174870:CDS	45.0%
	GTTTCCTCTAATGACCATCC+TGG	-	chr8_1:6079039-6079058	Msa1174870:five_prime_UTR	45.0%
	TTAGGAGGTCACAAATGCCT+TGG	-	chr8_1:6079215-6079234	Msa1174870:five_prime_UTR	45.0%
!!!	AAATTATATATTTTTTTTCA+TGG	-	chr8_1:6075657-6075676	Msa1174870:intron	5.0%
	CCTGAAGCTGAACAACGATC+TGG	+	chr8_1:6075812-6075831	None:intergenic	50.0%
	CCAGATCGTTGTTCAGCTTC+AGG	-	chr8_1:6075809-6075828	Msa1174870:intron	50.0%
	AGAGGGTCCCAACTCATGAA+TGG	+	chr8_1:6076126-6076145	None:intergenic	50.0%
!	GTGTGGTGGGGGTATATTGT+TGG	-	chr8_1:6077408-6077427	Msa1174870:intron	50.0%
!	GTGGTGGGGGTATATTGTTG+GGG	-	chr8_1:6077410-6077429	Msa1174870:intron	50.0%
!	GCAACCCGAAGCATTAAGAG+AGG	+	chr8_1:6077910-6077929	None:intergenic	50.0%
	ATTGTAGTCGTTGGATCGCC+AGG	-	chr8_1:6078351-6078370	Msa1174870:CDS	50.0%
	ATCCTCGACAACAGCAACAC+TGG	+	chr8_1:6078402-6078421	None:intergenic	50.0%
!	CACCAGTGTTGCTGTTGTCG+AGG	-	chr8_1:6078397-6078416	Msa1174870:CDS	55.0%
!	GGGGGAATTCTAGAAGGAGG+AGG	-	chr8_1:6078964-6078983	Msa1174870:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	6074997	6079455	6074997	ID=Msa1174870;Name=Msa1174870
chr8_1	mRNA	6074997	6079455	6074997	ID=Msa1174870-mRNA-1;Parent=Msa1174870;Name=Msa1174870-mRNA-1;_AED=0.25;_eAED=0.25;_QI=445\|0.8\|1\|1\|1\|1\|6\|378\|237
chr8_1	exon	6074997	6075527	6074997	ID=Msa1174870-mRNA-1:exon:2116;Parent=Msa1174870-mRNA-1
chr8_1	exon	6076391	6076627	6076391	ID=Msa1174870-mRNA-1:exon:2115;Parent=Msa1174870-mRNA-1
chr8_1	exon	6077295	6077351	6077295	ID=Msa1174870-mRNA-1:exon:2114;Parent=Msa1174870-mRNA-1
chr8_1	exon	6078349	6078416	6078349	ID=Msa1174870-mRNA-1:exon:2113;Parent=Msa1174870-mRNA-1
chr8_1	exon	6078559	6078652	6078559	ID=Msa1174870-mRNA-1:exon:2112;Parent=Msa1174870-mRNA-1
chr8_1	exon	6078906	6079455	6078906	ID=Msa1174870-mRNA-1:exon:2111;Parent=Msa1174870-mRNA-1
chr8_1	five_prime_UTR	6079011	6079455	6079011	ID=Msa1174870-mRNA-1:five_prime_utr;Parent=Msa1174870-mRNA-1
chr8_1	CDS	6078906	6079010	6078906	ID=Msa1174870-mRNA-1:cds;Parent=Msa1174870-mRNA-1
chr8_1	CDS	6078559	6078652	6078559	ID=Msa1174870-mRNA-1:cds;Parent=Msa1174870-mRNA-1
chr8_1	CDS	6078349	6078416	6078349	ID=Msa1174870-mRNA-1:cds;Parent=Msa1174870-mRNA-1
chr8_1	CDS	6077295	6077351	6077295	ID=Msa1174870-mRNA-1:cds;Parent=Msa1174870-mRNA-1
chr8_1	CDS	6076391	6076627	6076391	ID=Msa1174870-mRNA-1:cds;Parent=Msa1174870-mRNA-1
chr8_1	CDS	6075375	6075527	6075375	ID=Msa1174870-mRNA-1:cds;Parent=Msa1174870-mRNA-1
chr8_1	three_prime_UTR	6074997	6075374	6074997	ID=Msa1174870-mRNA-1:three_prime_utr;Parent=Msa1174870-mRNA-1

Gene Sequence

>Msa1174870

ATGGCTAATGAAGGAAACAAAAGCAATGATTTCTATGCAGTTTTGGGATTGAATAAGGAATGCTCTGATTCAGAACTAAGGAATGCTTATAAGAAACTTGCACTGAAATGGCATCCAGATCGTTGTTCAGCTTCAGGGAATGTGAAGTTTGTGGAAGAAGCTAAGAAGAAATTTCAGGCAATTCAAGAAGCCTATTCTGTTTTATCTGACTCGAACAAGAGATTAATGTACGACGTTGGAGTTTACGACAGTGATGATGACGAAAATGGTATGGGAGACTTTCTGAACGAAATGGTTACAATGATGAGCCAAACTAAATCAAATGAAAATGGAGAGGAGAGCTTTGAGGAGTTGCAACAGTTATTTGATGATATGTTTCAAGCGGATATCGGATTAAATGGAAGCACCTCTCTTAATGCTTCGGGTTGCTCCACTTCATCTACTTTCATGACTTTCGGTGAAAGCGGGAATTCAAATAAGCGCAATTCTACTCAGATGAATTATGGGAAGGCAGAGGATTCTTCTAGTTTTGGTCCAAATTACCAGAACTTCTGTTTTGGGACAGATGAAGCACCTTCAAGAAGATGTGGAGAAGGAAAAGGGGGAATTCTAGAAGGAGGAGGTAGCAAAAAACAAAGAAATGGAAGAAAACAAAAAATCTCATGTGGACATGATGTTTCCTCTAATGACCATCCTGGAATTTCTGCTAATTGA

Protein sequence

>Msa1174870

MANEGNKSNDFYAVLGLNKECSDSELRNAYKKLALKWHPDRCSASGNVKFVEEAKKKFQAIQEAYSVLSDSNKRLMYDVGVYDSDDDENGMGDFLNEMVTMMSQTKSNENGEESFEELQQLFDDMFQADIGLNGSTSLNASGCSTSSTFMTFGESGNSNKRNSTQMNYGKAEDSSSFGPNYQNFCFGTDEAPSRRCGEGKGGILEGGGSKKQRNGRKQKISCGHDVSSNDHPGISAN*