AlfalfaGEDB | Msa0650900

M. sativa cultivar zhongmu-4 / Msa0650900

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0650900	AES90005.1	96.629	89	3	0	1	89	1	89	1.02e-53	172

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0650900	G7JHI8	96.629	89	3	0	1	89	1	89	4.89e-54	172

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0005350	Msa0650900	0.800076	1.707309e-48	-8.615850e-47
Msa0025740	Msa0650900	0.800339	1.509828e-48	-8.615850e-47
Msa0033340	Msa0650900	0.814657	1.378301e-51	-8.615850e-47
Msa0044710	Msa0650900	0.817244	3.645467e-52	-8.615850e-47
Msa0053900	Msa0650900	0.804724	1.882444e-49	-8.615850e-47
Msa0069310	Msa0650900	0.807024	6.185496e-50	-8.615850e-47
Msa0071240	Msa0650900	0.804863	1.761402e-49	-8.615850e-47
Msa0078430	Msa0650900	0.809602	1.744477e-50	-8.615850e-47
Msa0094920	Msa0650900	0.832185	1.091714e-55	-8.615850e-47
Msa0095010	Msa0650900	0.806512	7.937008e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0650900	MtrunA17_Chr4g0043191	96.629	89	3	0	1	89	1	89	9.42e-58	172
Msa0650900	MtrunA17_Chr4g0043181	80.899	89	13	1	1	89	1	85	5.05e-40	127
Msa0650900	MtrunA17_Chr8g0376661	79.545	88	18	0	1	88	1	88	1.65e-36	118
Msa0650900	MtrunA17_Chr5g0434291	77.528	89	20	0	1	89	1	89	2.91e-36	117

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0650900	AT1G30260.1	60.417	48	18	1	1	48	1	47	3.72e-15	65.1

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Find 12 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACTGCATTGGTAGAAG+AGG	0.557920	4_3:+58177474	None:intergenic
AACAATCACTGAAGAGGATA+TGG	0.331678	4_3:-58177572	Msa0650900:CDS
ATCACGCTAACATACTGTGG+TGG	0.690677	4_3:+58177622	None:intergenic
CACAGTATGTTAGCGTGATG+AGG	0.569645	4_3:-58177618	Msa0650900:CDS
CCAATGCAGTTGTTAATTCT+AGG	0.257861	4_3:-58177465	Msa0650900:CDS
CCTAGAATTAACAACTGCAT+TGG	0.551602	4_3:+58177465	None:intergenic
CTCATCACGCTAACATACTG+TGG	0.654266	4_3:+58177619	None:intergenic
GATGGAAACAATCACTGAAG+AGG	0.591147	4_3:-58177578	Msa0650900:CDS
GCGTAGAACATCAAAGATGA+TGG	0.510830	4_3:-58177596	Msa0650900:CDS
GTGTTCTATGAACTTCCTTC+AGG	0.381163	4_3:+58177437	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAATCACTGAAGAGGATA+TGG	-	chr4_3:58177509-58177528	Msa0650900:CDS	35.0%
CCTAGAATTAACAACTGCAT+TGG	+	chr4_3:58177619-58177638	None:intergenic	35.0%
CCAATGCAGTTGTTAATTCT+AGG	-	chr4_3:58177616-58177635	Msa0650900:CDS	35.0%
TAATTCTAGGTACTTCCTGA+AGG	-	chr4_3:58177629-58177648	Msa0650900:CDS	35.0%
GCGTAGAACATCAAAGATGA+TGG	-	chr4_3:58177485-58177504	Msa0650900:CDS	40.0%
GATGGAAACAATCACTGAAG+AGG	-	chr4_3:58177503-58177522	Msa0650900:CDS	40.0%
GTGTTCTATGAACTTCCTTC+AGG	+	chr4_3:58177647-58177666	None:intergenic	40.0%
ATCACGCTAACATACTGTGG+TGG	+	chr4_3:58177462-58177481	None:intergenic	45.0%
CTCATCACGCTAACATACTG+TGG	+	chr4_3:58177465-58177484	None:intergenic	45.0%
CACAGTATGTTAGCGTGATG+AGG	-	chr4_3:58177463-58177482	Msa0650900:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	58177417	58177686	58177417	ID=Msa0650900;Name=Msa0650900
chr4_3	mRNA	58177417	58177686	58177417	ID=Msa0650900-mRNA-1;Parent=Msa0650900;Name=Msa0650900-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|89
chr4_3	exon	58177417	58177686	58177417	ID=Msa0650900-mRNA-1:exon:15342;Parent=Msa0650900-mRNA-1
chr4_3	CDS	58177417	58177686	58177417	ID=Msa0650900-mRNA-1:cds;Parent=Msa0650900-mRNA-1

Gene Sequence	>Msa0650900 ATGGCGAATTCTCGCATTGCAAGGTTTTTCATGGAGGTGGCACCACCACAGTATGTTAGCGTGATGAGGCGTAGAACATCAAAGATGATGGAAACAATCACTGAAGAGGATATGGAAATGAGTTCAAATGATAGTGTAATTCCACCACCTAAAAGTTCATCAACTTCATCACTTGCTGCTTCTGCATGTGCCTCTTCTACCAATGCAGTTGTTAATTCTAGGTACTTCCTGAAGGAAGTTCATAGAACACTCTCAAGTTTGAATCACTGA
Protein sequence	>Msa0650900 MANSRIARFFMEVAPPQYVSVMRRRTSKMMETITEEDMEMSSNDSVIPPPKSSSTSSLAASACASSTNAVVNSRYFLKEVHRTLSSLNH*