AlfalfaGEDB | Msa0154770

M. sativa cultivar zhongmu-4 / Msa0154770

KEGG: -
GO: -
PFAM: PF13640.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0154770	XP_003592342.3	95.804	286	10	2	1	286	1	284	0.0	549

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0154770	I3SR39	95.804	286	10	2	1	286	1	284	0.0	549

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0154770	-0.845234	4.611598e-59	-8.615850e-47
Msa0003470	Msa0154770	0.807477	4.958198e-50	-8.615850e-47
Msa0006290	Msa0154770	0.822217	2.666100e-53	-8.615850e-47
Msa0008850	Msa0154770	0.822778	1.974930e-53	-8.615850e-47
Msa0013050	Msa0154770	-0.817256	3.623531e-52	-8.615850e-47
Msa0027540	Msa0154770	0.816201	6.248901e-52	-8.615850e-47
Msa0027580	Msa0154770	0.831959	1.242131e-55	-8.615850e-47
Msa0030380	Msa0154770	0.846457	2.146384e-59	-8.615850e-47
Msa0032610	Msa0154770	0.809442	1.888115e-50	-8.615850e-47
Msa0041970	Msa0154770	0.819435	1.163215e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0154770	MtrunA17_Chr1g0204021	95.804	286	10	2	1	286	1	284	0.0	549

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0154770	AT5G51880.1	67.660	235	72	2	52	286	20	250	1.46e-107	312

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Find 66 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAACAACCGACCCAAAA+TGG	0.337133	1_4:-19537252	Msa0154770:CDS
AACCTATATAATCTAATATT+TGG	0.132507	1_4:+19536854	None:intergenic
AAGAAATAGCATTGTGGCTG+AGG	0.471528	1_4:-19535896	Msa0154770:intron
AAGAGAGGTATATCGTCAAG+CGG	0.696308	1_4:-19537372	Msa0154770:CDS
AAGAGTGCCATCCCTTCAAT+GGG	0.530149	1_4:+19535759	None:intergenic
AATTTGAAAAGTTCTAGTCA+AGG	0.624516	1_4:+19535364	None:intergenic
ACACACCTCGTAGGGAGGAA+GGG	0.587796	1_4:-19537312	Msa0154770:CDS
ACCTGGTGAAGTTAAATCCA+AGG	0.588470	1_4:-19535995	Msa0154770:CDS
ACGAGATTTCGATCTCTTCA+CGG	0.469965	1_4:-19537188	Msa0154770:intron
ACGATACACACCTCGTAGGG+AGG	0.666557	1_4:-19537317	Msa0154770:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGATATCGAAGCTTACAAA+AGG	-	chr1_4:19535439-19535458	Msa0154770:three_prime_UTR	30.0%
TGGGAAAAGAAAAAGAAGAA+GGG	+	chr1_4:19535704-19535723	None:intergenic	30.0%
TTGGGAAAAGAAAAAGAAGA+AGG	+	chr1_4:19535705-19535724	None:intergenic	30.0%
ATGAAAAGAAATTCTGAACC+TGG	+	chr1_4:19535764-19535783	None:intergenic	30.0%
TTTACTCATCAAGGAAGTTT+AGG	-	chr1_4:19535828-19535847	Msa0154770:intron	30.0%
TAGTTGTATAAGAACCTGTT+TGG	-	chr1_4:19536060-19536079	Msa0154770:CDS	30.0%
TAAGCTATTTATCCAAACAG+GGG	-	chr1_4:19536547-19536566	Msa0154770:intron	30.0%
CTAAGTTGTTTATGCAAACA+AGG	-	chr1_4:19536570-19536589	Msa0154770:intron	30.0%
AAATGCATGACAATTAGTGA+AGG	+	chr1_4:19536718-19536737	None:intergenic	30.0%
ATACCAGAGAATTTGATACA+AGG	+	chr1_4:19537221-19537240	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	19535373	19537496	19535373	ID=Msa0154770;Name=Msa0154770
chr1_4	mRNA	19535373	19537496	19535373	ID=Msa0154770-mRNA-1;Parent=Msa0154770;Name=Msa0154770-mRNA-1;_AED=0.19;_eAED=0.19;_QI=41\|1\|1\|1\|1\|1\|4\|292\|286
chr1_4	exon	19535373	19535787	19535373	ID=Msa0154770-mRNA-1:exon:7193;Parent=Msa0154770-mRNA-1
chr1_4	exon	19535897	19536119	19535897	ID=Msa0154770-mRNA-1:exon:7192;Parent=Msa0154770-mRNA-1
chr1_4	exon	19536857	19537104	19536857	ID=Msa0154770-mRNA-1:exon:7191;Parent=Msa0154770-mRNA-1
chr1_4	exon	19537189	19537496	19537189	ID=Msa0154770-mRNA-1:exon:7190;Parent=Msa0154770-mRNA-1
chr1_4	five_prime_UTR	19537456	19537496	19537456	ID=Msa0154770-mRNA-1:five_prime_utr;Parent=Msa0154770-mRNA-1
chr1_4	CDS	19537189	19537455	19537189	ID=Msa0154770-mRNA-1:cds;Parent=Msa0154770-mRNA-1
chr1_4	CDS	19536857	19537104	19536857	ID=Msa0154770-mRNA-1:cds;Parent=Msa0154770-mRNA-1
chr1_4	CDS	19535897	19536119	19535897	ID=Msa0154770-mRNA-1:cds;Parent=Msa0154770-mRNA-1
chr1_4	CDS	19535665	19535787	19535665	ID=Msa0154770-mRNA-1:cds;Parent=Msa0154770-mRNA-1
chr1_4	three_prime_UTR	19535373	19535664	19535373	ID=Msa0154770-mRNA-1:three_prime_utr;Parent=Msa0154770-mRNA-1

Gene Sequence

>Msa0154770

ATGGATCCTTCAAAATTATTTCTTTTTGATATCGAAGCTTACAAAAGGCGGTCTGAAATCGAAGAGAGGTATATCGTCAAGCGGTTCTTCAGGGAGCGACGAAACCAAATAGAGGAACGATACACACCTCGTAGGGAGGAAGGGAGCGTAAGGAGGAAGAGAAAAATGGGAGAGAGAAAAGAAACAACAACCGACCCAAAATGGCCTTCCATCAAACCCAAAAACAATCTTCACATCACTCGCTTACGAGATTTCGATCTCTTCACGGTTCAGAATTTCTTTTCATCTTATGAATCAAAGGCATTCATTGAAACTGCTGAGGGAATTGGTTTTACTCATCAAGGAAGTTTAGGTCCAGCGAAAGGTGAAGCTTATAGAGATAATGATCGAATTTCGGTGAATGATCCTGTTCTTGCTGATTCTATTTGGGATTCTGGACTTAACAAGCTTTTTTCTGATATAAGAATTCGAGGGAAAGCTGCTGTAGGTTTGAATCCAAATATTAGATTATATAGGTACAAAGTTGGTCAACGGTTTGGACGACATATCGATGAGAGTAATGATCTTGGAGATGGAAAAAGAACTCGTTATACTTTGCTAGTTTATTTGAGTGGCGGACCTGGTGAAGTTAAATCCAAGGCTAAAAATGATTCCAGCAAACCTACAGATTCATCTTCTGATCGTTTAGTTGGAGGAGAAACAGTCTTCTATGGTTCAAGAAATAGCATTGTGGCTGAGGTTGCTCCCATTGAAGGGATGGCACTCTTGCACATTCATGGAGATAAATGCTTGTTACATGAAGCCAGAAATGTTACCAAAGGTGTAAAATATGTCTTTCGTTCGGATGTTGTATTTGCTTGA

Protein sequence

>Msa0154770

MDPSKLFLFDIEAYKRRSEIEERYIVKRFFRERRNQIEERYTPRREEGSVRRKRKMGERKETTTDPKWPSIKPKNNLHITRLRDFDLFTVQNFFSSYESKAFIETAEGIGFTHQGSLGPAKGEAYRDNDRISVNDPVLADSIWDSGLNKLFSDIRIRGKAAVGLNPNIRLYRYKVGQRFGRHIDESNDLGDGKRTRYTLLVYLSGGPGEVKSKAKNDSSKPTDSSSDRLVGGETVFYGSRNSIVAEVAPIEGMALLHIHGDKCLLHEARNVTKGVKYVFRSDVVFA*