AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0425380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0425380 MtrunA17_Chr3g0127451 99.254 134 1 0 1 134 1 134 4.56e-98 277
Msa0425380 MtrunA17_Chr4g0010061 66.667 132 44 0 1 132 14 145 3.04e-64 192
Msa0425380 MtrunA17_Chr8g0346311 58.824 136 53 1 1 133 1 136 1.94e-56 172
Msa0425380 MtrunA17_Chr7g0276081 57.037 135 56 1 2 134 3 137 1.96e-45 144
Msa0425380 MtrunA17_Chr5g0419651 60.714 84 33 0 1 84 1 84 2.25e-34 116
Msa0425380 MtrunA17_Chr3g0134591 34.783 138 81 1 3 131 2 139 2.84e-26 96.3
Msa0425380 MtrunA17_Chr3g0115151 42.500 80 45 1 1 80 457 535 4.45e-18 79.3
Msa0425380 MtrunA17_Chr3g0115131 45.333 75 40 1 6 80 517 590 3.69e-17 76.6
Msa0425380 MtrunA17_Chr5g0424491 40.000 80 47 1 1 80 513 591 2.19e-16 74.3
Msa0425380 MtrunA17_Chr6g0486891 42.857 70 39 1 12 81 50 118 7.23e-16 68.6
Msa0425380 MtrunA17_Chr4g0019781 34.568 81 53 0 3 83 137 217 2.81e-13 63.9
Msa0425380 MtrunA17_Chr8g0361931 38.356 73 44 1 6 78 7 78 1.57e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0425380 AT5G53560.1 79.851 134 27 0 1 134 1 134 4.91e-79 229
Msa0425380 AT2G32720.1 66.667 132 44 0 1 132 1 132 6.94e-67 199
Msa0425380 AT5G48810.1 60.294 136 52 1 1 134 1 136 1.02e-60 183
Msa0425380 AT2G32720.2 70.270 111 33 0 22 132 22 132 3.86e-55 169
Msa0425380 AT2G46650.1 51.938 129 60 1 7 133 4 132 2.62e-38 126
Msa0425380 AT1G26340.1 44.094 127 61 2 6 127 6 127 5.33e-36 120
Msa0425380 AT1G37130.1 42.500 80 45 1 1 80 538 616 7.21e-19 82.0
Msa0425380 AT1G60660.1 43.421 76 42 1 5 80 46 120 1.46e-17 73.6
Msa0425380 AT1G77760.1 40.000 80 47 1 1 80 541 619 3.90e-17 77.0
Msa0425380 AT3G61580.1 38.158 76 46 1 3 78 5 79 8.37e-13 64.3
Msa0425380 AT2G46210.1 38.571 70 42 1 9 78 11 79 4.09e-11 59.7
Msa0425380 AT5G09680.3 32.530 83 56 0 3 85 170 252 5.00e-11 58.9

Find 62 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCACAATCCAACATTATT+AGG 0.187332 3_2:-75762041 None:intergenic
TCAAATCTCTTCTTCACTTT+GGG 0.187989 3_2:-75759362 None:intergenic
CCGGGATGATCCTCCATAAA+AGG 0.201719 3_2:-75761480 None:intergenic
CCCCATAAACAGGAAAATTA+AGG 0.220364 3_2:-75762168 None:intergenic
CCTGGTGCCTCTCCTGATAT+TGG 0.232035 3_2:+75761808 Msa0425380:CDS
GACAGTGTTTAGTTTCTTCT+TGG 0.292754 3_2:+75761941 Msa0425380:three_prime_UTR
CTGGTGCCTCTCCTGATATT+GGG 0.297310 3_2:+75761809 Msa0425380:CDS
AAGAAGGAACTACTCTTTCT+TGG 0.318516 3_2:-75761858 None:intergenic
GAGAATAAGCCAGCAATCTT+TGG 0.319930 3_2:-75759753 None:intergenic
GTTTAGCATCTTTGTTTCAT+TGG 0.320261 3_2:+75762077 Msa0425380:three_prime_UTR
GTCAAATCTCTTCTTCACTT+TGG 0.341887 3_2:-75759363 None:intergenic
GTGGAACGTAGGTGCGCTTA+AGG 0.375400 3_2:-75761724 None:intergenic
CACTTTGCATCCCCATAAAC+AGG 0.379042 3_2:-75762178 None:intergenic
GATTGCTGGCTTATTCTCTC+TGG 0.386144 3_2:+75759758 Msa0425380:CDS
CAACTTTCTGGTAATGGTAC+TGG 0.390866 3_2:+75761999 Msa0425380:three_prime_UTR
TCCCACATCTTCAAAATCAT+TGG 0.392408 3_2:-75761633 None:intergenic
GGTGTCTTGAGCATTGAAAC+TGG 0.406699 3_2:+75761962 Msa0425380:three_prime_UTR
TCATCGAAAGTGTGAAGCTT+TGG 0.412470 3_2:-75759710 None:intergenic
CAAAGGCTAAGCCCAATATC+AGG 0.426178 3_2:-75761820 None:intergenic
GGTCTGTTAGCTATTTATAG+AGG 0.431918 3_2:+75761907 Msa0425380:three_prime_UTR
CCCATAAACAGGAAAATTAA+GGG 0.432212 3_2:-75762167 None:intergenic
TCTCTTCTTCACTTTGGGGT+TGG 0.435970 3_2:-75759357 None:intergenic
CAAATCTCTTCTTCACTTTG+GGG 0.437199 3_2:-75759361 None:intergenic
AATTACCACTTCCGGTCCTT+GGG 0.440955 3_2:-75759417 None:intergenic
GAATTACCACTTCCGGTCCT+TGG 0.443825 3_2:-75759418 None:intergenic
CGGGATGATCCTCCATAAAA+GGG 0.446544 3_2:-75761479 None:intergenic
TGGAACGTAGGTGCGCTTAA+GGG 0.449787 3_2:-75761723 None:intergenic
GAGGTTCTGTTGTCTGCAAC+AGG 0.466883 3_2:+75761510 Msa0425380:CDS
ACAACAAGACCAAAGATTGC+TGG 0.472357 3_2:+75759744 Msa0425380:CDS
CGCTTAAGGGGAACAGTGGA+TGG 0.472619 3_2:-75761710 None:intergenic
ACAAATTCCGAAGTCTTGTC+CGG 0.476643 3_2:-75761767 None:intergenic
CAAGAGGGAATTACCACTTC+CGG 0.485709 3_2:-75759425 None:intergenic
ATCATATTGCACCCTTGTGA+GGG 0.492456 3_2:+75761885 Msa0425380:three_prime_UTR
TGATTCTGCTAGAGAAATGA+TGG 0.506307 3_2:+75761661 Msa0425380:CDS
CAACAGAACCTCATCACCTC+CGG 0.513023 3_2:-75761499 None:intergenic
CTGGCTTATTCTCTCTGGAA+AGG 0.542076 3_2:+75759763 Msa0425380:CDS
CATCATATTGCACCCTTGTG+AGG 0.542934 3_2:+75761884 Msa0425380:three_prime_UTR
AATCTCCCAAGGACCGGAAG+TGG 0.548565 3_2:+75759412 Msa0425380:five_prime_UTR
CCAATATCAGGAGAGGCACC+AGG 0.552438 3_2:-75761808 None:intergenic
GGAACGTAGGTGCGCTTAAG+GGG 0.553895 3_2:-75761722 None:intergenic
TGCAACTAGATTATTGTCCC+AGG 0.555593 3_2:+75762211 Msa0425380:three_prime_UTR
GTGCAATATGATGATAAAGA+AGG 0.560027 3_2:-75761874 None:intergenic
ATTTATAGAGGGTCATTCAA+AGG 0.568496 3_2:+75761919 Msa0425380:three_prime_UTR
ACTCAGAATCTCCCAAGGAC+CGG 0.572963 3_2:+75759406 Msa0425380:five_prime_UTR
GTCTGTTAGCTATTTATAGA+GGG 0.573849 3_2:+75761908 Msa0425380:three_prime_UTR
GGATCATCCCGGAGGTGATG+AGG 0.581222 3_2:+75761491 Msa0425380:CDS
AACAGAACCTCATCACCTCC+GGG 0.588122 3_2:-75761498 None:intergenic
GTACAATCCGGACAAGACTT+CGG 0.598055 3_2:+75761760 Msa0425380:CDS
TCAGCAATCTCAGTACAATC+CGG 0.604833 3_2:+75761748 Msa0425380:CDS
ATGATGGACAAATACTACAT+TGG 0.610366 3_2:+75761677 Msa0425380:CDS
TAGCTAACAGACCCCTCACA+AGG 0.620015 3_2:-75761897 None:intergenic
TTGTACTGAGATTGCTGAGG+TGG 0.630347 3_2:-75761743 None:intergenic
GGTGTAGTTTCTGACAACAA+AGG 0.643712 3_2:-75761837 None:intergenic
TCATATTGCACCCTTGTGAG+GGG 0.653808 3_2:+75761886 Msa0425380:three_prime_UTR
TCATCACTCAGAATCTCCCA+AGG 0.658723 3_2:+75759401 Msa0425380:five_prime_UTR
GCTAAGCCCAATATCAGGAG+AGG 0.665710 3_2:-75761815 None:intergenic
GGTGCGCTTAAGGGGAACAG+TGG 0.672781 3_2:-75761714 None:intergenic
GGGATGATCCTCCATAAAAG+GGG 0.676795 3_2:-75761478 None:intergenic
AGCTAACAGACCCCTCACAA+GGG 0.690074 3_2:-75761896 None:intergenic
GGATTGTACTGAGATTGCTG+AGG 0.741733 3_2:-75761746 None:intergenic
TTTATGGAGGATCATCCCGG+AGG 0.751420 3_2:+75761483 Msa0425380:CDS
AGATTGCTGAGGTGGAACGT+AGG 0.774863 3_2:-75761735 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTAAAGAATCAATAAAAA+GGG - chr3_2:75759590-75759609 None:intergenic 15.0%
!!! ATTCAGATAAAATTAATTTG+TGG + chr3_2:75759622-75759641 Msa0425380:intron 15.0%
!!! TTCAGATAAAATTAATTTGT+GGG + chr3_2:75759623-75759642 Msa0425380:intron 15.0%
!! AGTTAAAATATTAGTACATA+TGG - chr3_2:75760594-75760613 None:intergenic 15.0%
!!! TATATTACATTTGTATTTTG+TGG + chr3_2:75760765-75760784 Msa0425380:intron 15.0%
!!! ATATTACATTTGTATTTTGT+GGG + chr3_2:75760766-75760785 Msa0425380:intron 15.0%
!! ATAAATTAATAACCCATTAT+AGG - chr3_2:75760993-75761012 None:intergenic 15.0%
!! TGTAGAAAAATTTATGTATT+TGG - chr3_2:75761373-75761392 None:intergenic 15.0%
!! ATAGTACAATATAATCAATA+TGG - chr3_2:75761585-75761604 None:intergenic 15.0%
!! TCTAAAGAATCAATAAAAAG+GGG - chr3_2:75759589-75759608 None:intergenic 20.0%
!! GATCTAAAGAATCAATAAAA+AGG - chr3_2:75759591-75759610 None:intergenic 20.0%
!! ACTACAAATGAATTACAATT+AGG - chr3_2:75759996-75760015 None:intergenic 20.0%
!! CAACATTCAATCTTAAAATT+AGG - chr3_2:75760067-75760086 None:intergenic 20.0%
!! TTGAATTATTCAAATCTGAA+CGG + chr3_2:75760353-75760372 Msa0425380:intron 20.0%
!! GAATAAGAAGAATGAATATT+AGG + chr3_2:75760714-75760733 Msa0425380:intron 20.0%
!!! TATTACATTTGTATTTTGTG+GGG + chr3_2:75760767-75760786 Msa0425380:intron 20.0%
!!! TTATTGGTATCCTTTTAAAA+TGG + chr3_2:75760921-75760940 Msa0425380:intron 20.0%
!!! TATAATGTTTTACAACTTTC+TGG + chr3_2:75761987-75762006 Msa0425380:three_prime_UTR 20.0%
!!! AGTGATCCTTTAAAAAAAAA+AGG - chr3_2:75762115-75762134 None:intergenic 20.0%
! TTCCATGATTGATTATTCTT+CGG + chr3_2:75759811-75759830 Msa0425380:intron 25.0%
!!! AAGATGAAGTTCAAGTTTTT+GGG + chr3_2:75759914-75759933 Msa0425380:intron 25.0%
!! TTTAAGATTGAATGTTGTTG+AGG + chr3_2:75760070-75760089 Msa0425380:intron 25.0%
!!! GTAGACAGATTACATTTTTT+AGG + chr3_2:75760157-75760176 Msa0425380:intron 25.0%
! TACAATAAGATCCAAACTTA+AGG - chr3_2:75760328-75760347 None:intergenic 25.0%
!! CGATGACTCATTTTATTATT+TGG - chr3_2:75760425-75760444 None:intergenic 25.0%
! TTTAACTGTCACGTAAAATA+TGG + chr3_2:75760607-75760626 Msa0425380:intron 25.0%
! TAAGTTAATCTACCTCTTAT+TGG + chr3_2:75760905-75760924 Msa0425380:intron 25.0%
! TTTAAAAGGATACCAATAAG+AGG - chr3_2:75760920-75760939 None:intergenic 25.0%
! AAGCATCTAAGCTATATAAA+AGG - chr3_2:75761153-75761172 None:intergenic 25.0%
!! AACGTTTAAAATAGTGAAAG+TGG - chr3_2:75761258-75761277 None:intergenic 25.0%
! AAGAACAAATTATCGTTAAG+AGG - chr3_2:75761433-75761452 None:intergenic 25.0%
! ATTTGTTCTTCAATCATGTT+AGG + chr3_2:75761443-75761462 Msa0425380:intron 25.0%
! TATGCTTATATCATTCGAAA+AGG + chr3_2:75761533-75761552 Msa0425380:intron 25.0%
! ATGCTTATATCATTCGAAAA+GGG + chr3_2:75761534-75761553 Msa0425380:intron 25.0%
! TACTATACTGTATCAATTAC+AGG + chr3_2:75761599-75761618 Msa0425380:intron 25.0%
! ACTATACTGTATCAATTACA+GGG + chr3_2:75761600-75761619 Msa0425380:intron 25.0%
! ACTGAAAAACCTAATAATGT+TGG + chr3_2:75762032-75762051 Msa0425380:three_prime_UTR 25.0%
! ATTCACAATCCAACATTATT+AGG - chr3_2:75762044-75762063 None:intergenic 25.0%
!!! CTCAAGCCTTTTTTTTTTAA+AGG + chr3_2:75762106-75762125 Msa0425380:three_prime_UTR 25.0%
TCAAATCTCTTCTTCACTTT+GGG - chr3_2:75759365-75759384 None:intergenic 30.0%
TTTGAAGGGAAAAAACAAGA+GGG - chr3_2:75759443-75759462 None:intergenic 30.0%
ATTTGAAGGGAAAAAACAAG+AGG - chr3_2:75759444-75759463 None:intergenic 30.0%
GGAAAAGAGAAGAATTTGAA+GGG - chr3_2:75759457-75759476 None:intergenic 30.0%
TAGAAAAGAATCGATGCAAA+GGG - chr3_2:75759478-75759497 None:intergenic 30.0%
TTAGAAAAGAATCGATGCAA+AGG - chr3_2:75759479-75759498 None:intergenic 30.0%
!!! GGTTTGACTTGAATTTTTCT+GGG + chr3_2:75759644-75759663 Msa0425380:intron 30.0%
TGGCAGTGAAAGAAAAAAAA+TGG + chr3_2:75759679-75759698 Msa0425380:intron 30.0%
TTCCGAAGAATAATCAATCA+TGG - chr3_2:75759816-75759835 None:intergenic 30.0%
!! GAAGATGAAGTTCAAGTTTT+TGG + chr3_2:75759913-75759932 Msa0425380:intron 30.0%
TGCATGTTACATATATTTCC+TGG + chr3_2:75759941-75759960 Msa0425380:intron 30.0%
TCAGTGTGGTTAACAAATTA+TGG + chr3_2:75760115-75760134 Msa0425380:intron 30.0%
AATAAGATCCAAACTTAAGG+TGG - chr3_2:75760325-75760344 None:intergenic 30.0%
ATTCATTCTTCTTACACGTT+CGG - chr3_2:75760698-75760717 None:intergenic 30.0%
!!! TTGGTATCCTTTTAAAATGG+AGG + chr3_2:75760924-75760943 Msa0425380:intron 30.0%
!!! TGGTATCCTTTTAAAATGGA+GGG + chr3_2:75760925-75760944 Msa0425380:intron 30.0%
AATAACCCATTATAGGAAGA+CGG - chr3_2:75760986-75761005 None:intergenic 30.0%
TAAAGAATCAAACTGTGGAA+AGG - chr3_2:75761029-75761048 None:intergenic 30.0%
AAGGATAAAGAATCAAACTG+TGG - chr3_2:75761034-75761053 None:intergenic 30.0%
GCTATATAAAAGGAAGATTG+AGG - chr3_2:75761143-75761162 None:intergenic 30.0%
TATAGCTTAGATGCTTTCAT+TGG + chr3_2:75761157-75761176 Msa0425380:intron 30.0%
TCTTATAGGAAGATAGGTTA+GGG - chr3_2:75761202-75761221 None:intergenic 30.0%
CTAATGTCTTATAGGAAGAT+AGG - chr3_2:75761208-75761227 None:intergenic 30.0%
AATTAGAATAGCCTCACAAT+TGG - chr3_2:75761298-75761317 None:intergenic 30.0%
!! AACCAATGATTTTGAAGATG+TGG + chr3_2:75761631-75761650 Msa0425380:CDS 30.0%
!! ACCAATGATTTTGAAGATGT+GGG + chr3_2:75761632-75761651 Msa0425380:CDS 30.0%
!! ATGATGGACAAATACTACAT+TGG + chr3_2:75761677-75761696 Msa0425380:CDS 30.0%
GTGCAATATGATGATAAAGA+AGG - chr3_2:75761877-75761896 None:intergenic 30.0%
GTCTGTTAGCTATTTATAGA+GGG + chr3_2:75761908-75761927 Msa0425380:three_prime_UTR 30.0%
ATTTATAGAGGGTCATTCAA+AGG + chr3_2:75761919-75761938 Msa0425380:three_prime_UTR 30.0%
!! GTTTTACAACTTTCTGGTAA+TGG + chr3_2:75761993-75762012 Msa0425380:three_prime_UTR 30.0%
! GTTTAGCATCTTTGTTTCAT+TGG + chr3_2:75762077-75762096 Msa0425380:three_prime_UTR 30.0%
! ACCCTTAATTTTCCTGTTTA+TGG + chr3_2:75762166-75762185 Msa0425380:three_prime_UTR 30.0%
CCCATAAACAGGAAAATTAA+GGG - chr3_2:75762170-75762189 None:intergenic 30.0%
! CCCTTAATTTTCCTGTTTAT+GGG + chr3_2:75762167-75762186 Msa0425380:three_prime_UTR 30.0%
! CCTTAATTTTCCTGTTTATG+GGG + chr3_2:75762168-75762187 Msa0425380:three_prime_UTR 30.0%
CAAATCTCTTCTTCACTTTG+GGG - chr3_2:75759364-75759383 None:intergenic 35.0%
GTCAAATCTCTTCTTCACTT+TGG - chr3_2:75759366-75759385 None:intergenic 35.0%
GGGAAAAGAGAAGAATTTGA+AGG - chr3_2:75759458-75759477 None:intergenic 35.0%
!!! GGGTTTGACTTGAATTTTTC+TGG + chr3_2:75759643-75759662 Msa0425380:intron 35.0%
!! TTTCTGGGTCTTTGATTTTG+TGG + chr3_2:75759659-75759678 Msa0425380:intron 35.0%
TTTGGTCATCTATGAGTAAG+AGG - chr3_2:75760407-75760426 None:intergenic 35.0%
CATGCAAAATTACTGGTGTT+AGG - chr3_2:75760467-75760486 None:intergenic 35.0%
TCTCTAGCATGCAAAATTAC+TGG - chr3_2:75760474-75760493 None:intergenic 35.0%
! TAATTTTGCATGCTAGAGAG+TGG + chr3_2:75760475-75760494 Msa0425380:intron 35.0%
GAGTAGAAGAATATAGCATC+TGG + chr3_2:75760500-75760519 Msa0425380:intron 35.0%
ACGACTAGTAATGCACATAA+AGG - chr3_2:75760557-75760576 None:intergenic 35.0%
TTCATTCTTCTTACACGTTC+GGG - chr3_2:75760697-75760716 None:intergenic 35.0%
!!! TATCCTTTTAAAATGGAGGG+AGG + chr3_2:75760928-75760947 Msa0425380:intron 35.0%
! TTTCCTCCCTCCATTTTAAA+AGG - chr3_2:75760934-75760953 None:intergenic 35.0%
AAAATGGAGGGAGGAAAAAA+AGG + chr3_2:75760937-75760956 Msa0425380:intron 35.0%
ATAAACCCGTCTTCCTATAA+TGG + chr3_2:75760977-75760996 Msa0425380:intron 35.0%
TAAACCCGTCTTCCTATAAT+GGG + chr3_2:75760978-75760997 Msa0425380:intron 35.0%
ATAACCCATTATAGGAAGAC+GGG - chr3_2:75760985-75761004 None:intergenic 35.0%
! TTAGTCAAATATGCTCTGAG+AGG + chr3_2:75761110-75761129 Msa0425380:intron 35.0%
CTTATAGGAAGATAGGTTAG+GGG - chr3_2:75761201-75761220 None:intergenic 35.0%
GTCTTATAGGAAGATAGGTT+AGG - chr3_2:75761203-75761222 None:intergenic 35.0%
! TCGACTAGCTAATGTCTTAT+AGG - chr3_2:75761216-75761235 None:intergenic 35.0%
TCCCACATCTTCAAAATCAT+TGG - chr3_2:75761636-75761655 None:intergenic 35.0%
TGATTCTGCTAGAGAAATGA+TGG + chr3_2:75761661-75761680 Msa0425380:CDS 35.0%
AAGAAGGAACTACTCTTTCT+TGG - chr3_2:75761861-75761880 None:intergenic 35.0%
GGTCTGTTAGCTATTTATAG+AGG + chr3_2:75761907-75761926 Msa0425380:three_prime_UTR 35.0%
! GACAGTGTTTAGTTTCTTCT+TGG + chr3_2:75761941-75761960 Msa0425380:three_prime_UTR 35.0%
CCCCATAAACAGGAAAATTA+AGG - chr3_2:75762171-75762190 None:intergenic 35.0%
! TCATCGAAAGTGTGAAGCTT+TGG - chr3_2:75759713-75759732 None:intergenic 40.0%
ACAACAAGACCAAAGATTGC+TGG + chr3_2:75759744-75759763 Msa0425380:CDS 40.0%
GAGAATAAGCCAGCAATCTT+TGG - chr3_2:75759756-75759775 None:intergenic 40.0%
ATGCTATGTCTAGATCAGTG+TGG + chr3_2:75760101-75760120 Msa0425380:intron 40.0%
GTCTACAAGTACAGAAGAAC+AGG - chr3_2:75760143-75760162 None:intergenic 40.0%
ACAAACGAAGCATGTCATGT+AGG - chr3_2:75760296-75760315 None:intergenic 40.0%
AAGATCCAAACTTAAGGTGG+TGG - chr3_2:75760322-75760341 None:intergenic 40.0%
AGATAGATCTCAAGATCTGC+AGG - chr3_2:75760847-75760866 None:intergenic 40.0%
ATCTGATGTTTGATGCCATG+TGG + chr3_2:75760863-75760882 Msa0425380:intron 40.0%
TCTGATGTTTGATGCCATGT+GGG + chr3_2:75760864-75760883 Msa0425380:intron 40.0%
! ATAGGAAGACGGGTTTATGA+AGG - chr3_2:75760975-75760994 None:intergenic 40.0%
AACTGTGGAAAGGAAGAGTA+TGG - chr3_2:75761019-75761038 None:intergenic 40.0%
CTAGTCGAAAATCTAGCAGA+AGG + chr3_2:75761228-75761247 Msa0425380:intron 40.0%
! ATGGTGCTCTGCATTTTTCA+GGG - chr3_2:75761321-75761340 None:intergenic 40.0%
! ATGCAGAGCACCATGTATAT+TGG + chr3_2:75761327-75761346 Msa0425380:intron 40.0%
! ACCATGTATATTGGCCTGAA+TGG + chr3_2:75761336-75761355 Msa0425380:intron 40.0%
ACCATTCAGGCCAATATACA+TGG - chr3_2:75761340-75761359 None:intergenic 40.0%
TCAGCAATCTCAGTACAATC+CGG + chr3_2:75761748-75761767 Msa0425380:CDS 40.0%
! ACAAATTCCGAAGTCTTGTC+CGG - chr3_2:75761770-75761789 None:intergenic 40.0%
! GATCAAGATTTTGCAGTTCC+TGG + chr3_2:75761790-75761809 Msa0425380:CDS 40.0%
GGTGTAGTTTCTGACAACAA+AGG - chr3_2:75761840-75761859 None:intergenic 40.0%
ATCATATTGCACCCTTGTGA+GGG + chr3_2:75761885-75761904 Msa0425380:three_prime_UTR 40.0%
!! CAACTTTCTGGTAATGGTAC+TGG + chr3_2:75761999-75762018 Msa0425380:three_prime_UTR 40.0%
!! TCTCTTCTTCACTTTGGGGT+TGG - chr3_2:75759360-75759379 None:intergenic 45.0%
TCATCACTCAGAATCTCCCA+AGG + chr3_2:75759401-75759420 Msa0425380:five_prime_UTR 45.0%
AATTACCACTTCCGGTCCTT+GGG - chr3_2:75759420-75759439 None:intergenic 45.0%
CAAGAGGGAATTACCACTTC+CGG - chr3_2:75759428-75759447 None:intergenic 45.0%
!! GATTGCTGGCTTATTCTCTC+TGG + chr3_2:75759758-75759777 Msa0425380:CDS 45.0%
! CTGGCTTATTCTCTCTGGAA+AGG + chr3_2:75759763-75759782 Msa0425380:CDS 45.0%
ACATGCAATGCACAAGATCC+AGG - chr3_2:75759962-75759981 None:intergenic 45.0%
GTCAACCACCACCTTAAGTT+TGG + chr3_2:75760314-75760333 Msa0425380:intron 45.0%
GTGCATTACTAGTCGTGTAC+TGG + chr3_2:75760561-75760580 Msa0425380:intron 45.0%
CTGCTCGCTTATGAAAGGTA+TGG - chr3_2:75760644-75760663 None:intergenic 45.0%
CGATACTGCTCGCTTATGAA+AGG - chr3_2:75760649-75760668 None:intergenic 45.0%
TTTGATGCCATGTGGGACTT+GGG + chr3_2:75760871-75760890 Msa0425380:intron 45.0%
CATTGTGTTTCGACGTCACA+GGG - chr3_2:75761062-75761081 None:intergenic 45.0%
CTGTGACGTCGAAACACAAT+GGG + chr3_2:75761061-75761080 Msa0425380:intron 45.0%
TGTGACGTCGAAACACAATG+GGG + chr3_2:75761062-75761081 Msa0425380:intron 45.0%
! AAACACAATGGGGAGAGTGT+TGG + chr3_2:75761072-75761091 Msa0425380:intron 45.0%
!! GTCAAATATGCTCTGAGAGG+TGG + chr3_2:75761113-75761132 Msa0425380:intron 45.0%
TAGGAAGATAGGTTAGGGGA+AGG - chr3_2:75761197-75761216 None:intergenic 45.0%
AACGTTGCCTGATATTGCGA+TGG + chr3_2:75761272-75761291 Msa0425380:intron 45.0%
CAATTGGCCATCGCAATATC+AGG - chr3_2:75761282-75761301 None:intergenic 45.0%
TATTGCGATGGCCAATTGTG+AGG + chr3_2:75761284-75761303 Msa0425380:intron 45.0%
! CATGGTGCTCTGCATTTTTC+AGG - chr3_2:75761322-75761341 None:intergenic 45.0%
TTGGCCTGAATGGTCACTAT+TGG + chr3_2:75761346-75761365 Msa0425380:intron 45.0%
TGGCCTGAATGGTCACTATT+GGG + chr3_2:75761347-75761366 Msa0425380:intron 45.0%
! GTACGATGTCACCCCTTTTA+TGG + chr3_2:75761467-75761486 Msa0425380:CDS 45.0%
GGGATGATCCTCCATAAAAG+GGG - chr3_2:75761481-75761500 None:intergenic 45.0%
CGGGATGATCCTCCATAAAA+GGG - chr3_2:75761482-75761501 None:intergenic 45.0%
! CCTTTTATGGAGGATCATCC+CGG + chr3_2:75761480-75761499 Msa0425380:CDS 45.0%
!! GGTATGCTACTAAAGCACTG+AGG + chr3_2:75761555-75761574 Msa0425380:intron 45.0%
TTGTACTGAGATTGCTGAGG+TGG - chr3_2:75761746-75761765 None:intergenic 45.0%
GGATTGTACTGAGATTGCTG+AGG - chr3_2:75761749-75761768 None:intergenic 45.0%
GTACAATCCGGACAAGACTT+CGG + chr3_2:75761760-75761779 Msa0425380:CDS 45.0%
CAAAGGCTAAGCCCAATATC+AGG - chr3_2:75761823-75761842 None:intergenic 45.0%
CATCATATTGCACCCTTGTG+AGG + chr3_2:75761884-75761903 Msa0425380:three_prime_UTR 45.0%
TCATATTGCACCCTTGTGAG+GGG + chr3_2:75761886-75761905 Msa0425380:three_prime_UTR 45.0%
!! GGTGTCTTGAGCATTGAAAC+TGG + chr3_2:75761962-75761981 Msa0425380:three_prime_UTR 45.0%
CACTTTGCATCCCCATAAAC+AGG - chr3_2:75762181-75762200 None:intergenic 45.0%
ACTCAGAATCTCCCAAGGAC+CGG + chr3_2:75759406-75759425 Msa0425380:five_prime_UTR 50.0%
GAATTACCACTTCCGGTCCT+TGG - chr3_2:75759421-75759440 None:intergenic 50.0%
GTTTGATGCCATGTGGGACT+TGG + chr3_2:75760870-75760889 Msa0425380:intron 50.0%
CCATTGTGTTTCGACGTCAC+AGG - chr3_2:75761063-75761082 None:intergenic 50.0%
CCTGTGACGTCGAAACACAA+TGG + chr3_2:75761060-75761079 Msa0425380:intron 50.0%
TGGCCCAATAGTGACCATTC+AGG - chr3_2:75761353-75761372 None:intergenic 50.0%
! CGATGTCACCCCTTTTATGG+AGG + chr3_2:75761470-75761489 Msa0425380:CDS 50.0%
CCGGGATGATCCTCCATAAA+AGG - chr3_2:75761483-75761502 None:intergenic 50.0%
TTTATGGAGGATCATCCCGG+AGG + chr3_2:75761483-75761502 Msa0425380:CDS 50.0%
AACAGAACCTCATCACCTCC+GGG - chr3_2:75761501-75761520 None:intergenic 50.0%
CAACAGAACCTCATCACCTC+CGG - chr3_2:75761502-75761521 None:intergenic 50.0%
GAGGTTCTGTTGTCTGCAAC+AGG + chr3_2:75761510-75761529 Msa0425380:CDS 50.0%
TGGAACGTAGGTGCGCTTAA+GGG - chr3_2:75761726-75761745 None:intergenic 50.0%
AGATTGCTGAGGTGGAACGT+AGG - chr3_2:75761738-75761757 None:intergenic 50.0%
CTGGTGCCTCTCCTGATATT+GGG + chr3_2:75761809-75761828 Msa0425380:CDS 50.0%
GCTAAGCCCAATATCAGGAG+AGG - chr3_2:75761818-75761837 None:intergenic 50.0%
AGCTAACAGACCCCTCACAA+GGG - chr3_2:75761899-75761918 None:intergenic 50.0%
TAGCTAACAGACCCCTCACA+AGG - chr3_2:75761900-75761919 None:intergenic 50.0%
AATCTCCCAAGGACCGGAAG+TGG + chr3_2:75759412-75759431 Msa0425380:five_prime_UTR 55.0%
ATGCCATGTGGGACTTGGGA+AGG + chr3_2:75760875-75760894 Msa0425380:intron 55.0%
TGCCATGTGGGACTTGGGAA+GGG + chr3_2:75760876-75760895 Msa0425380:intron 55.0%
TTCCCTTCCCAAGTCCCACA+TGG - chr3_2:75760881-75760900 None:intergenic 55.0%
TCGACGTCACAGGGAACAGA+AGG - chr3_2:75761053-75761072 None:intergenic 55.0%
CGCTTAAGGGGAACAGTGGA+TGG - chr3_2:75761713-75761732 None:intergenic 55.0%
GGAACGTAGGTGCGCTTAAG+GGG - chr3_2:75761725-75761744 None:intergenic 55.0%
GTGGAACGTAGGTGCGCTTA+AGG - chr3_2:75761727-75761746 None:intergenic 55.0%
!! CCAATATCAGGAGAGGCACC+AGG - chr3_2:75761811-75761830 None:intergenic 55.0%
CCTGGTGCCTCTCCTGATAT+TGG + chr3_2:75761808-75761827 Msa0425380:CDS 55.0%
GGATCATCCCGGAGGTGATG+AGG + chr3_2:75761491-75761510 Msa0425380:CDS 60.0%
GGTGCGCTTAAGGGGAACAG+TGG - chr3_2:75761717-75761736 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 75759356 75762230 75759356 ID=Msa0425380;Name=Msa0425380
chr3_2 mRNA 75759356 75762230 75759356 ID=Msa0425380-mRNA-1;Parent=Msa0425380;Name=Msa0425380-mRNA-1;_AED=0.01;_eAED=0.01;_QI=91|1|1|1|0.66|0.5|4|359|134
chr3_2 exon 75759356 75759433 75759356 ID=Msa0425380-mRNA-1:exon:19409;Parent=Msa0425380-mRNA-1
chr3_2 exon 75759685 75759784 75759685 ID=Msa0425380-mRNA-1:exon:19410;Parent=Msa0425380-mRNA-1
chr3_2 exon 75761465 75761531 75761465 ID=Msa0425380-mRNA-1:exon:19411;Parent=Msa0425380-mRNA-1
chr3_2 exon 75761621 75762230 75761621 ID=Msa0425380-mRNA-1:exon:19412;Parent=Msa0425380-mRNA-1
chr3_2 five_prime_UTR 75759356 75759433 75759356 ID=Msa0425380-mRNA-1:five_prime_utr;Parent=Msa0425380-mRNA-1
chr3_2 five_prime_UTR 75759685 75759697 75759685 ID=Msa0425380-mRNA-1:five_prime_utr;Parent=Msa0425380-mRNA-1
chr3_2 CDS 75759698 75759784 75759698 ID=Msa0425380-mRNA-1:cds;Parent=Msa0425380-mRNA-1
chr3_2 CDS 75761465 75761531 75761465 ID=Msa0425380-mRNA-1:cds;Parent=Msa0425380-mRNA-1
chr3_2 CDS 75761621 75761871 75761621 ID=Msa0425380-mRNA-1:cds;Parent=Msa0425380-mRNA-1
chr3_2 three_prime_UTR 75761872 75762230 75761872 ID=Msa0425380-mRNA-1:three_prime_utr;Parent=Msa0425380-mRNA-1
Gene Sequence

>Msa0425380

ATGGCTTCAGATCCAAAGCTTCACACTTTCGATGATGTGTCAAAGCACAACAAGACCAAAGATTGCTGGCTTATTCTCTCTGGAAAGGTGTACGATGTCACCCCTTTTATGGAGGATCATCCCGGAGGTGATGAGGTTCTGTTGTCTGCAACAGGGAAAGATGCAACCAATGATTTTGAAGATGTGGGACACAGTGATTCTGCTAGAGAAATGATGGACAAATACTACATTGGTGAGATTGATCCATCCACTGTTCCCCTTAAGCGCACCTACGTTCCACCTCAGCAATCTCAGTACAATCCGGACAAGACTTCGGAATTTGTGATCAAGATTTTGCAGTTCCTGGTGCCTCTCCTGATATTGGGCTTAGCCTTTGTTGTCAGAAACTACACCAAGAAAGAGTAG
Protein sequence

>Msa0425380

MASDPKLHTFDDVSKHNKTKDCWLILSGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKRTYVPPQQSQYNPDKTSEFVIKILQFLVPLLILGLAFVVRNYTKKE*