AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1397070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1397070 MtrunA17_Chr3g0127451 99.254 134 1 0 1 134 1 134 4.56e-98 277
Msa1397070 MtrunA17_Chr4g0010061 66.667 132 44 0 1 132 14 145 3.04e-64 192
Msa1397070 MtrunA17_Chr8g0346311 58.824 136 53 1 1 133 1 136 1.94e-56 172
Msa1397070 MtrunA17_Chr7g0276081 57.037 135 56 1 2 134 3 137 1.96e-45 144
Msa1397070 MtrunA17_Chr5g0419651 60.714 84 33 0 1 84 1 84 2.25e-34 116
Msa1397070 MtrunA17_Chr3g0134591 34.783 138 81 1 3 131 2 139 2.84e-26 96.3
Msa1397070 MtrunA17_Chr3g0115151 42.500 80 45 1 1 80 457 535 4.45e-18 79.3
Msa1397070 MtrunA17_Chr3g0115131 45.333 75 40 1 6 80 517 590 3.69e-17 76.6
Msa1397070 MtrunA17_Chr5g0424491 40.000 80 47 1 1 80 513 591 2.19e-16 74.3
Msa1397070 MtrunA17_Chr6g0486891 42.857 70 39 1 12 81 50 118 7.23e-16 68.6
Msa1397070 MtrunA17_Chr4g0019781 34.568 81 53 0 3 83 137 217 2.81e-13 63.9
Msa1397070 MtrunA17_Chr8g0361931 38.356 73 44 1 6 78 7 78 1.57e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1397070 AT5G53560.1 79.851 134 27 0 1 134 1 134 4.91e-79 229
Msa1397070 AT2G32720.1 66.667 132 44 0 1 132 1 132 6.94e-67 199
Msa1397070 AT5G48810.1 60.294 136 52 1 1 134 1 136 1.02e-60 183
Msa1397070 AT2G32720.2 70.270 111 33 0 22 132 22 132 3.86e-55 169
Msa1397070 AT2G46650.1 51.938 129 60 1 7 133 4 132 2.62e-38 126
Msa1397070 AT1G26340.1 44.094 127 61 2 6 127 6 127 5.33e-36 120
Msa1397070 AT1G37130.1 42.500 80 45 1 1 80 538 616 7.21e-19 82.0
Msa1397070 AT1G60660.1 43.421 76 42 1 5 80 46 120 1.46e-17 73.6
Msa1397070 AT1G77760.1 40.000 80 47 1 1 80 541 619 3.90e-17 77.0
Msa1397070 AT3G61580.1 38.158 76 46 1 3 78 5 79 8.37e-13 64.3
Msa1397070 AT2G46210.1 38.571 70 42 1 9 78 11 79 4.09e-11 59.7
Msa1397070 AT5G09680.3 32.530 83 56 0 3 85 170 252 5.00e-11 58.9

Find 62 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCACAATCCAACATTATT+AGG 0.187332 tig0022126:-30012 None:intergenic
TCAAATCTCTTCTTCACTTT+GGG 0.187989 tig0022126:-27333 None:intergenic
CCGGGATGATCCTCCATAAA+AGG 0.201719 tig0022126:-29451 None:intergenic
CCCCATAAACAGGAAAATTA+AGG 0.220364 tig0022126:-30139 None:intergenic
CCTGGTGCCTCTCCTGATAT+TGG 0.232035 tig0022126:+29779 Msa1397070:CDS
GACAGTGTTTAGTTTCTTCT+TGG 0.292754 tig0022126:+29912 Msa1397070:three_prime_UTR
CTGGTGCCTCTCCTGATATT+GGG 0.297310 tig0022126:+29780 Msa1397070:CDS
AAGAAGGAACTACTCTTTCT+TGG 0.318516 tig0022126:-29829 None:intergenic
GAGAATAAGCCAGCAATCTT+TGG 0.319930 tig0022126:-27724 None:intergenic
GTTTAGCATCTTTGTTTCAT+TGG 0.320261 tig0022126:+30048 Msa1397070:three_prime_UTR
GTCAAATCTCTTCTTCACTT+TGG 0.341887 tig0022126:-27334 None:intergenic
GTGGAACGTAGGTGCGCTTA+AGG 0.375400 tig0022126:-29695 None:intergenic
CACTTTGCATCCCCATAAAC+AGG 0.379042 tig0022126:-30149 None:intergenic
GATTGCTGGCTTATTCTCTC+TGG 0.386144 tig0022126:+27729 Msa1397070:CDS
CAACTTTCTGGTAATGGTAC+TGG 0.390866 tig0022126:+29970 Msa1397070:three_prime_UTR
TCCCACATCTTCAAAATCAT+TGG 0.392408 tig0022126:-29604 None:intergenic
GGTGTCTTGAGCATTGAAAC+TGG 0.406699 tig0022126:+29933 Msa1397070:three_prime_UTR
TCATCGAAAGTGTGAAGCTT+TGG 0.412470 tig0022126:-27681 None:intergenic
CAAAGGCTAAGCCCAATATC+AGG 0.426178 tig0022126:-29791 None:intergenic
GGTCTGTTAGCTATTTATAG+AGG 0.431918 tig0022126:+29878 Msa1397070:three_prime_UTR
CCCATAAACAGGAAAATTAA+GGG 0.432212 tig0022126:-30138 None:intergenic
TCTCTTCTTCACTTTGGGGT+TGG 0.435970 tig0022126:-27328 None:intergenic
CAAATCTCTTCTTCACTTTG+GGG 0.437199 tig0022126:-27332 None:intergenic
AATTACCACTTCCGGTCCTT+GGG 0.440955 tig0022126:-27388 None:intergenic
GAATTACCACTTCCGGTCCT+TGG 0.443825 tig0022126:-27389 None:intergenic
CGGGATGATCCTCCATAAAA+GGG 0.446544 tig0022126:-29450 None:intergenic
TGGAACGTAGGTGCGCTTAA+GGG 0.449787 tig0022126:-29694 None:intergenic
GAGGTTCTGTTGTCTGCAAC+AGG 0.466883 tig0022126:+29481 Msa1397070:CDS
ACAACAAGACCAAAGATTGC+TGG 0.472357 tig0022126:+27715 Msa1397070:CDS
CGCTTAAGGGGAACAGTGGA+TGG 0.472619 tig0022126:-29681 None:intergenic
ACAAATTCCGAAGTCTTGTC+CGG 0.476643 tig0022126:-29738 None:intergenic
CAAGAGGGAATTACCACTTC+CGG 0.485709 tig0022126:-27396 None:intergenic
ATCATATTGCACCCTTGTGA+GGG 0.492456 tig0022126:+29856 Msa1397070:three_prime_UTR
TGATTCTGCTAGAGAAATGA+TGG 0.506307 tig0022126:+29632 Msa1397070:CDS
CAACAGAACCTCATCACCTC+CGG 0.513023 tig0022126:-29470 None:intergenic
CTGGCTTATTCTCTCTGGAA+AGG 0.542076 tig0022126:+27734 Msa1397070:CDS
CATCATATTGCACCCTTGTG+AGG 0.542934 tig0022126:+29855 Msa1397070:three_prime_UTR
AATCTCCCAAGGACCGGAAG+TGG 0.548565 tig0022126:+27383 Msa1397070:five_prime_UTR
CCAATATCAGGAGAGGCACC+AGG 0.552438 tig0022126:-29779 None:intergenic
GGAACGTAGGTGCGCTTAAG+GGG 0.553895 tig0022126:-29693 None:intergenic
TGCAACTAGATTATTGTCCC+AGG 0.555593 tig0022126:+30182 Msa1397070:three_prime_UTR
GTGCAATATGATGATAAAGA+AGG 0.560027 tig0022126:-29845 None:intergenic
ATTTATAGAGGGTCATTCAA+AGG 0.568496 tig0022126:+29890 Msa1397070:three_prime_UTR
ACTCAGAATCTCCCAAGGAC+CGG 0.572963 tig0022126:+27377 Msa1397070:five_prime_UTR
GTCTGTTAGCTATTTATAGA+GGG 0.573849 tig0022126:+29879 Msa1397070:three_prime_UTR
GGATCATCCCGGAGGTGATG+AGG 0.581222 tig0022126:+29462 Msa1397070:CDS
AACAGAACCTCATCACCTCC+GGG 0.588122 tig0022126:-29469 None:intergenic
GTACAATCCGGACAAGACTT+CGG 0.598055 tig0022126:+29731 Msa1397070:CDS
TCAGCAATCTCAGTACAATC+CGG 0.604833 tig0022126:+29719 Msa1397070:CDS
ATGATGGACAAATACTACAT+TGG 0.610366 tig0022126:+29648 Msa1397070:CDS
TAGCTAACAGACCCCTCACA+AGG 0.620015 tig0022126:-29868 None:intergenic
TTGTACTGAGATTGCTGAGG+TGG 0.630347 tig0022126:-29714 None:intergenic
GGTGTAGTTTCTGACAACAA+AGG 0.643712 tig0022126:-29808 None:intergenic
TCATATTGCACCCTTGTGAG+GGG 0.653808 tig0022126:+29857 Msa1397070:three_prime_UTR
TCATCACTCAGAATCTCCCA+AGG 0.658723 tig0022126:+27372 Msa1397070:five_prime_UTR
GCTAAGCCCAATATCAGGAG+AGG 0.665710 tig0022126:-29786 None:intergenic
GGTGCGCTTAAGGGGAACAG+TGG 0.672781 tig0022126:-29685 None:intergenic
GGGATGATCCTCCATAAAAG+GGG 0.676795 tig0022126:-29449 None:intergenic
AGCTAACAGACCCCTCACAA+GGG 0.690074 tig0022126:-29867 None:intergenic
GGATTGTACTGAGATTGCTG+AGG 0.741733 tig0022126:-29717 None:intergenic
TTTATGGAGGATCATCCCGG+AGG 0.751420 tig0022126:+29454 Msa1397070:CDS
AGATTGCTGAGGTGGAACGT+AGG 0.774863 tig0022126:-29706 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTAAAGAATCAATAAAAA+GGG - tig0022126:27561-27580 None:intergenic 15.0%
!!! ATTCAGATAAAATTAATTTG+TGG + tig0022126:27593-27612 Msa1397070:intron 15.0%
!!! TTCAGATAAAATTAATTTGT+GGG + tig0022126:27594-27613 Msa1397070:intron 15.0%
!! AGTTAAAATATTAGTACATA+TGG - tig0022126:28565-28584 None:intergenic 15.0%
!!! TATATTACATTTGTATTTTG+TGG + tig0022126:28736-28755 Msa1397070:intron 15.0%
!!! ATATTACATTTGTATTTTGT+GGG + tig0022126:28737-28756 Msa1397070:intron 15.0%
!! ATAAATTAATAACCCATTAT+AGG - tig0022126:28964-28983 None:intergenic 15.0%
!! TGTAGAAAAATTTATGTATT+TGG - tig0022126:29344-29363 None:intergenic 15.0%
!! ATAGTACAATATAATCAATA+TGG - tig0022126:29556-29575 None:intergenic 15.0%
!! TCTAAAGAATCAATAAAAAG+GGG - tig0022126:27560-27579 None:intergenic 20.0%
!! GATCTAAAGAATCAATAAAA+AGG - tig0022126:27562-27581 None:intergenic 20.0%
!! ACTACAAATGAATTACAATT+AGG - tig0022126:27967-27986 None:intergenic 20.0%
!! CAACATTCAATCTTAAAATT+AGG - tig0022126:28038-28057 None:intergenic 20.0%
!! TTGAATTATTCAAATCTGAA+CGG + tig0022126:28324-28343 Msa1397070:intron 20.0%
!! GAATAAGAAGAATGAATATT+AGG + tig0022126:28685-28704 Msa1397070:intron 20.0%
!!! TATTACATTTGTATTTTGTG+GGG + tig0022126:28738-28757 Msa1397070:intron 20.0%
!!! TTATTGGTATCCTTTTAAAA+TGG + tig0022126:28892-28911 Msa1397070:intron 20.0%
!!! TATAATGTTTTACAACTTTC+TGG + tig0022126:29958-29977 Msa1397070:three_prime_UTR 20.0%
!!! AGTGATCCTTTAAAAAAAAA+AGG - tig0022126:30086-30105 None:intergenic 20.0%
! TTCCATGATTGATTATTCTT+CGG + tig0022126:27782-27801 Msa1397070:intron 25.0%
!!! AAGATGAAGTTCAAGTTTTT+GGG + tig0022126:27885-27904 Msa1397070:intron 25.0%
!! TTTAAGATTGAATGTTGTTG+AGG + tig0022126:28041-28060 Msa1397070:intron 25.0%
!!! GTAGACAGATTACATTTTTT+AGG + tig0022126:28128-28147 Msa1397070:intron 25.0%
! TACAATAAGATCCAAACTTA+AGG - tig0022126:28299-28318 None:intergenic 25.0%
!! CGATGACTCATTTTATTATT+TGG - tig0022126:28396-28415 None:intergenic 25.0%
! TTTAACTGTCACGTAAAATA+TGG + tig0022126:28578-28597 Msa1397070:intron 25.0%
! TAAGTTAATCTACCTCTTAT+TGG + tig0022126:28876-28895 Msa1397070:intron 25.0%
! TTTAAAAGGATACCAATAAG+AGG - tig0022126:28891-28910 None:intergenic 25.0%
! AAGCATCTAAGCTATATAAA+AGG - tig0022126:29124-29143 None:intergenic 25.0%
!! AACGTTTAAAATAGTGAAAG+TGG - tig0022126:29229-29248 None:intergenic 25.0%
! AAGAACAAATTATCGTTAAG+AGG - tig0022126:29404-29423 None:intergenic 25.0%
! ATTTGTTCTTCAATCATGTT+AGG + tig0022126:29414-29433 Msa1397070:intron 25.0%
! TATGCTTATATCATTCGAAA+AGG + tig0022126:29504-29523 Msa1397070:intron 25.0%
! ATGCTTATATCATTCGAAAA+GGG + tig0022126:29505-29524 Msa1397070:intron 25.0%
! TACTATACTGTATCAATTAC+AGG + tig0022126:29570-29589 Msa1397070:intron 25.0%
! ACTATACTGTATCAATTACA+GGG + tig0022126:29571-29590 Msa1397070:intron 25.0%
! ACTGAAAAACCTAATAATGT+TGG + tig0022126:30003-30022 Msa1397070:three_prime_UTR 25.0%
! ATTCACAATCCAACATTATT+AGG - tig0022126:30015-30034 None:intergenic 25.0%
!!! CTCAAGCCTTTTTTTTTTAA+AGG + tig0022126:30077-30096 Msa1397070:three_prime_UTR 25.0%
TCAAATCTCTTCTTCACTTT+GGG - tig0022126:27336-27355 None:intergenic 30.0%
TTTGAAGGGAAAAAACAAGA+GGG - tig0022126:27414-27433 None:intergenic 30.0%
ATTTGAAGGGAAAAAACAAG+AGG - tig0022126:27415-27434 None:intergenic 30.0%
GGAAAAGAGAAGAATTTGAA+GGG - tig0022126:27428-27447 None:intergenic 30.0%
TAGAAAAGAATCGATGCAAA+GGG - tig0022126:27449-27468 None:intergenic 30.0%
TTAGAAAAGAATCGATGCAA+AGG - tig0022126:27450-27469 None:intergenic 30.0%
!!! GGTTTGACTTGAATTTTTCT+GGG + tig0022126:27615-27634 Msa1397070:intron 30.0%
TGGCAGTGAAAGAAAAAAAA+TGG + tig0022126:27650-27669 Msa1397070:intron 30.0%
TTCCGAAGAATAATCAATCA+TGG - tig0022126:27787-27806 None:intergenic 30.0%
!! GAAGATGAAGTTCAAGTTTT+TGG + tig0022126:27884-27903 Msa1397070:intron 30.0%
TGCATGTTACATATATTTCC+TGG + tig0022126:27912-27931 Msa1397070:intron 30.0%
TCAGTGTGGTTAACAAATTA+TGG + tig0022126:28086-28105 Msa1397070:intron 30.0%
AATAAGATCCAAACTTAAGG+TGG - tig0022126:28296-28315 None:intergenic 30.0%
ATTCATTCTTCTTACACGTT+CGG - tig0022126:28669-28688 None:intergenic 30.0%
!!! TTGGTATCCTTTTAAAATGG+AGG + tig0022126:28895-28914 Msa1397070:intron 30.0%
!!! TGGTATCCTTTTAAAATGGA+GGG + tig0022126:28896-28915 Msa1397070:intron 30.0%
AATAACCCATTATAGGAAGA+CGG - tig0022126:28957-28976 None:intergenic 30.0%
TAAAGAATCAAACTGTGGAA+AGG - tig0022126:29000-29019 None:intergenic 30.0%
AAGGATAAAGAATCAAACTG+TGG - tig0022126:29005-29024 None:intergenic 30.0%
GCTATATAAAAGGAAGATTG+AGG - tig0022126:29114-29133 None:intergenic 30.0%
TATAGCTTAGATGCTTTCAT+TGG + tig0022126:29128-29147 Msa1397070:intron 30.0%
TCTTATAGGAAGATAGGTTA+GGG - tig0022126:29173-29192 None:intergenic 30.0%
CTAATGTCTTATAGGAAGAT+AGG - tig0022126:29179-29198 None:intergenic 30.0%
AATTAGAATAGCCTCACAAT+TGG - tig0022126:29269-29288 None:intergenic 30.0%
!! AACCAATGATTTTGAAGATG+TGG + tig0022126:29602-29621 Msa1397070:CDS 30.0%
!! ACCAATGATTTTGAAGATGT+GGG + tig0022126:29603-29622 Msa1397070:CDS 30.0%
!! ATGATGGACAAATACTACAT+TGG + tig0022126:29648-29667 Msa1397070:CDS 30.0%
GTGCAATATGATGATAAAGA+AGG - tig0022126:29848-29867 None:intergenic 30.0%
GTCTGTTAGCTATTTATAGA+GGG + tig0022126:29879-29898 Msa1397070:three_prime_UTR 30.0%
ATTTATAGAGGGTCATTCAA+AGG + tig0022126:29890-29909 Msa1397070:three_prime_UTR 30.0%
!! GTTTTACAACTTTCTGGTAA+TGG + tig0022126:29964-29983 Msa1397070:three_prime_UTR 30.0%
! GTTTAGCATCTTTGTTTCAT+TGG + tig0022126:30048-30067 Msa1397070:three_prime_UTR 30.0%
! ACCCTTAATTTTCCTGTTTA+TGG + tig0022126:30137-30156 Msa1397070:three_prime_UTR 30.0%
CCCATAAACAGGAAAATTAA+GGG - tig0022126:30141-30160 None:intergenic 30.0%
! CCCTTAATTTTCCTGTTTAT+GGG + tig0022126:30138-30157 Msa1397070:three_prime_UTR 30.0%
! CCTTAATTTTCCTGTTTATG+GGG + tig0022126:30139-30158 Msa1397070:three_prime_UTR 30.0%
CAAATCTCTTCTTCACTTTG+GGG - tig0022126:27335-27354 None:intergenic 35.0%
GTCAAATCTCTTCTTCACTT+TGG - tig0022126:27337-27356 None:intergenic 35.0%
GGGAAAAGAGAAGAATTTGA+AGG - tig0022126:27429-27448 None:intergenic 35.0%
!!! GGGTTTGACTTGAATTTTTC+TGG + tig0022126:27614-27633 Msa1397070:intron 35.0%
!! TTTCTGGGTCTTTGATTTTG+TGG + tig0022126:27630-27649 Msa1397070:intron 35.0%
TTTGGTCATCTATGAGTAAG+AGG - tig0022126:28378-28397 None:intergenic 35.0%
CATGCAAAATTACTGGTGTT+AGG - tig0022126:28438-28457 None:intergenic 35.0%
TCTCTAGCATGCAAAATTAC+TGG - tig0022126:28445-28464 None:intergenic 35.0%
! TAATTTTGCATGCTAGAGAG+TGG + tig0022126:28446-28465 Msa1397070:intron 35.0%
GAGTAGAAGAATATAGCATC+TGG + tig0022126:28471-28490 Msa1397070:intron 35.0%
ACGACTAGTAATGCACATAA+AGG - tig0022126:28528-28547 None:intergenic 35.0%
TTCATTCTTCTTACACGTTC+GGG - tig0022126:28668-28687 None:intergenic 35.0%
!!! TATCCTTTTAAAATGGAGGG+AGG + tig0022126:28899-28918 Msa1397070:intron 35.0%
! TTTCCTCCCTCCATTTTAAA+AGG - tig0022126:28905-28924 None:intergenic 35.0%
AAAATGGAGGGAGGAAAAAA+AGG + tig0022126:28908-28927 Msa1397070:intron 35.0%
ATAAACCCGTCTTCCTATAA+TGG + tig0022126:28948-28967 Msa1397070:intron 35.0%
TAAACCCGTCTTCCTATAAT+GGG + tig0022126:28949-28968 Msa1397070:intron 35.0%
ATAACCCATTATAGGAAGAC+GGG - tig0022126:28956-28975 None:intergenic 35.0%
! TTAGTCAAATATGCTCTGAG+AGG + tig0022126:29081-29100 Msa1397070:intron 35.0%
CTTATAGGAAGATAGGTTAG+GGG - tig0022126:29172-29191 None:intergenic 35.0%
GTCTTATAGGAAGATAGGTT+AGG - tig0022126:29174-29193 None:intergenic 35.0%
! TCGACTAGCTAATGTCTTAT+AGG - tig0022126:29187-29206 None:intergenic 35.0%
TCCCACATCTTCAAAATCAT+TGG - tig0022126:29607-29626 None:intergenic 35.0%
TGATTCTGCTAGAGAAATGA+TGG + tig0022126:29632-29651 Msa1397070:CDS 35.0%
AAGAAGGAACTACTCTTTCT+TGG - tig0022126:29832-29851 None:intergenic 35.0%
GGTCTGTTAGCTATTTATAG+AGG + tig0022126:29878-29897 Msa1397070:three_prime_UTR 35.0%
! GACAGTGTTTAGTTTCTTCT+TGG + tig0022126:29912-29931 Msa1397070:three_prime_UTR 35.0%
CCCCATAAACAGGAAAATTA+AGG - tig0022126:30142-30161 None:intergenic 35.0%
! TCATCGAAAGTGTGAAGCTT+TGG - tig0022126:27684-27703 None:intergenic 40.0%
ACAACAAGACCAAAGATTGC+TGG + tig0022126:27715-27734 Msa1397070:CDS 40.0%
GAGAATAAGCCAGCAATCTT+TGG - tig0022126:27727-27746 None:intergenic 40.0%
ATGCTATGTCTAGATCAGTG+TGG + tig0022126:28072-28091 Msa1397070:intron 40.0%
GTCTACAAGTACAGAAGAAC+AGG - tig0022126:28114-28133 None:intergenic 40.0%
ACAAACGAAGCATGTCATGT+AGG - tig0022126:28267-28286 None:intergenic 40.0%
AAGATCCAAACTTAAGGTGG+TGG - tig0022126:28293-28312 None:intergenic 40.0%
AGATAGATCTCAAGATCTGC+AGG - tig0022126:28818-28837 None:intergenic 40.0%
ATCTGATGTTTGATGCCATG+TGG + tig0022126:28834-28853 Msa1397070:intron 40.0%
TCTGATGTTTGATGCCATGT+GGG + tig0022126:28835-28854 Msa1397070:intron 40.0%
! ATAGGAAGACGGGTTTATGA+AGG - tig0022126:28946-28965 None:intergenic 40.0%
AACTGTGGAAAGGAAGAGTA+TGG - tig0022126:28990-29009 None:intergenic 40.0%
CTAGTCGAAAATCTAGCAGA+AGG + tig0022126:29199-29218 Msa1397070:intron 40.0%
! ATGGTGCTCTGCATTTTTCA+GGG - tig0022126:29292-29311 None:intergenic 40.0%
! ATGCAGAGCACCATGTATAT+TGG + tig0022126:29298-29317 Msa1397070:intron 40.0%
! ACCATGTATATTGGCCTGAA+TGG + tig0022126:29307-29326 Msa1397070:intron 40.0%
ACCATTCAGGCCAATATACA+TGG - tig0022126:29311-29330 None:intergenic 40.0%
TCAGCAATCTCAGTACAATC+CGG + tig0022126:29719-29738 Msa1397070:CDS 40.0%
! ACAAATTCCGAAGTCTTGTC+CGG - tig0022126:29741-29760 None:intergenic 40.0%
! GATCAAGATTTTGCAGTTCC+TGG + tig0022126:29761-29780 Msa1397070:CDS 40.0%
GGTGTAGTTTCTGACAACAA+AGG - tig0022126:29811-29830 None:intergenic 40.0%
ATCATATTGCACCCTTGTGA+GGG + tig0022126:29856-29875 Msa1397070:three_prime_UTR 40.0%
!! CAACTTTCTGGTAATGGTAC+TGG + tig0022126:29970-29989 Msa1397070:three_prime_UTR 40.0%
!! TCTCTTCTTCACTTTGGGGT+TGG - tig0022126:27331-27350 None:intergenic 45.0%
TCATCACTCAGAATCTCCCA+AGG + tig0022126:27372-27391 Msa1397070:five_prime_UTR 45.0%
AATTACCACTTCCGGTCCTT+GGG - tig0022126:27391-27410 None:intergenic 45.0%
CAAGAGGGAATTACCACTTC+CGG - tig0022126:27399-27418 None:intergenic 45.0%
!! GATTGCTGGCTTATTCTCTC+TGG + tig0022126:27729-27748 Msa1397070:CDS 45.0%
! CTGGCTTATTCTCTCTGGAA+AGG + tig0022126:27734-27753 Msa1397070:CDS 45.0%
ACATGCAATGCACAAGATCC+AGG - tig0022126:27933-27952 None:intergenic 45.0%
GTCAACCACCACCTTAAGTT+TGG + tig0022126:28285-28304 Msa1397070:intron 45.0%
GTGCATTACTAGTCGTGTAC+TGG + tig0022126:28532-28551 Msa1397070:intron 45.0%
CTGCTCGCTTATGAAAGGTA+TGG - tig0022126:28615-28634 None:intergenic 45.0%
CGATACTGCTCGCTTATGAA+AGG - tig0022126:28620-28639 None:intergenic 45.0%
TTTGATGCCATGTGGGACTT+GGG + tig0022126:28842-28861 Msa1397070:intron 45.0%
CATTGTGTTTCGACGTCACA+GGG - tig0022126:29033-29052 None:intergenic 45.0%
CTGTGACGTCGAAACACAAT+GGG + tig0022126:29032-29051 Msa1397070:intron 45.0%
TGTGACGTCGAAACACAATG+GGG + tig0022126:29033-29052 Msa1397070:intron 45.0%
! AAACACAATGGGGAGAGTGT+TGG + tig0022126:29043-29062 Msa1397070:intron 45.0%
!! GTCAAATATGCTCTGAGAGG+TGG + tig0022126:29084-29103 Msa1397070:intron 45.0%
TAGGAAGATAGGTTAGGGGA+AGG - tig0022126:29168-29187 None:intergenic 45.0%
AACGTTGCCTGATATTGCGA+TGG + tig0022126:29243-29262 Msa1397070:intron 45.0%
CAATTGGCCATCGCAATATC+AGG - tig0022126:29253-29272 None:intergenic 45.0%
TATTGCGATGGCCAATTGTG+AGG + tig0022126:29255-29274 Msa1397070:intron 45.0%
! CATGGTGCTCTGCATTTTTC+AGG - tig0022126:29293-29312 None:intergenic 45.0%
TTGGCCTGAATGGTCACTAT+TGG + tig0022126:29317-29336 Msa1397070:intron 45.0%
TGGCCTGAATGGTCACTATT+GGG + tig0022126:29318-29337 Msa1397070:intron 45.0%
! GTACGATGTCACCCCTTTTA+TGG + tig0022126:29438-29457 Msa1397070:CDS 45.0%
GGGATGATCCTCCATAAAAG+GGG - tig0022126:29452-29471 None:intergenic 45.0%
CGGGATGATCCTCCATAAAA+GGG - tig0022126:29453-29472 None:intergenic 45.0%
! CCTTTTATGGAGGATCATCC+CGG + tig0022126:29451-29470 Msa1397070:CDS 45.0%
!! GGTATGCTACTAAAGCACTG+AGG + tig0022126:29526-29545 Msa1397070:intron 45.0%
TTGTACTGAGATTGCTGAGG+TGG - tig0022126:29717-29736 None:intergenic 45.0%
GGATTGTACTGAGATTGCTG+AGG - tig0022126:29720-29739 None:intergenic 45.0%
GTACAATCCGGACAAGACTT+CGG + tig0022126:29731-29750 Msa1397070:CDS 45.0%
CAAAGGCTAAGCCCAATATC+AGG - tig0022126:29794-29813 None:intergenic 45.0%
CATCATATTGCACCCTTGTG+AGG + tig0022126:29855-29874 Msa1397070:three_prime_UTR 45.0%
TCATATTGCACCCTTGTGAG+GGG + tig0022126:29857-29876 Msa1397070:three_prime_UTR 45.0%
!! GGTGTCTTGAGCATTGAAAC+TGG + tig0022126:29933-29952 Msa1397070:three_prime_UTR 45.0%
CACTTTGCATCCCCATAAAC+AGG - tig0022126:30152-30171 None:intergenic 45.0%
ACTCAGAATCTCCCAAGGAC+CGG + tig0022126:27377-27396 Msa1397070:five_prime_UTR 50.0%
GAATTACCACTTCCGGTCCT+TGG - tig0022126:27392-27411 None:intergenic 50.0%
GTTTGATGCCATGTGGGACT+TGG + tig0022126:28841-28860 Msa1397070:intron 50.0%
CCATTGTGTTTCGACGTCAC+AGG - tig0022126:29034-29053 None:intergenic 50.0%
CCTGTGACGTCGAAACACAA+TGG + tig0022126:29031-29050 Msa1397070:intron 50.0%
TGGCCCAATAGTGACCATTC+AGG - tig0022126:29324-29343 None:intergenic 50.0%
! CGATGTCACCCCTTTTATGG+AGG + tig0022126:29441-29460 Msa1397070:CDS 50.0%
CCGGGATGATCCTCCATAAA+AGG - tig0022126:29454-29473 None:intergenic 50.0%
TTTATGGAGGATCATCCCGG+AGG + tig0022126:29454-29473 Msa1397070:CDS 50.0%
AACAGAACCTCATCACCTCC+GGG - tig0022126:29472-29491 None:intergenic 50.0%
CAACAGAACCTCATCACCTC+CGG - tig0022126:29473-29492 None:intergenic 50.0%
GAGGTTCTGTTGTCTGCAAC+AGG + tig0022126:29481-29500 Msa1397070:CDS 50.0%
TGGAACGTAGGTGCGCTTAA+GGG - tig0022126:29697-29716 None:intergenic 50.0%
AGATTGCTGAGGTGGAACGT+AGG - tig0022126:29709-29728 None:intergenic 50.0%
CTGGTGCCTCTCCTGATATT+GGG + tig0022126:29780-29799 Msa1397070:CDS 50.0%
GCTAAGCCCAATATCAGGAG+AGG - tig0022126:29789-29808 None:intergenic 50.0%
AGCTAACAGACCCCTCACAA+GGG - tig0022126:29870-29889 None:intergenic 50.0%
TAGCTAACAGACCCCTCACA+AGG - tig0022126:29871-29890 None:intergenic 50.0%
AATCTCCCAAGGACCGGAAG+TGG + tig0022126:27383-27402 Msa1397070:five_prime_UTR 55.0%
ATGCCATGTGGGACTTGGGA+AGG + tig0022126:28846-28865 Msa1397070:intron 55.0%
TGCCATGTGGGACTTGGGAA+GGG + tig0022126:28847-28866 Msa1397070:intron 55.0%
TTCCCTTCCCAAGTCCCACA+TGG - tig0022126:28852-28871 None:intergenic 55.0%
TCGACGTCACAGGGAACAGA+AGG - tig0022126:29024-29043 None:intergenic 55.0%
CGCTTAAGGGGAACAGTGGA+TGG - tig0022126:29684-29703 None:intergenic 55.0%
GGAACGTAGGTGCGCTTAAG+GGG - tig0022126:29696-29715 None:intergenic 55.0%
GTGGAACGTAGGTGCGCTTA+AGG - tig0022126:29698-29717 None:intergenic 55.0%
!! CCAATATCAGGAGAGGCACC+AGG - tig0022126:29782-29801 None:intergenic 55.0%
CCTGGTGCCTCTCCTGATAT+TGG + tig0022126:29779-29798 Msa1397070:CDS 55.0%
GGATCATCCCGGAGGTGATG+AGG + tig0022126:29462-29481 Msa1397070:CDS 60.0%
GGTGCGCTTAAGGGGAACAG+TGG - tig0022126:29688-29707 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0022126 gene 27327 30201 27327 ID=Msa1397070;Name=Msa1397070
tig0022126 mRNA 27327 30201 27327 ID=Msa1397070-mRNA-1;Parent=Msa1397070;Name=Msa1397070-mRNA-1;_AED=0.01;_eAED=0.01;_QI=91|1|1|1|0.66|0.5|4|359|134
tig0022126 exon 27327 27404 27327 ID=Msa1397070-mRNA-1:exon:15836;Parent=Msa1397070-mRNA-1
tig0022126 exon 27656 27755 27656 ID=Msa1397070-mRNA-1:exon:15837;Parent=Msa1397070-mRNA-1
tig0022126 exon 29436 29502 29436 ID=Msa1397070-mRNA-1:exon:15838;Parent=Msa1397070-mRNA-1
tig0022126 exon 29592 30201 29592 ID=Msa1397070-mRNA-1:exon:15839;Parent=Msa1397070-mRNA-1
tig0022126 five_prime_UTR 27327 27404 27327 ID=Msa1397070-mRNA-1:five_prime_utr;Parent=Msa1397070-mRNA-1
tig0022126 five_prime_UTR 27656 27668 27656 ID=Msa1397070-mRNA-1:five_prime_utr;Parent=Msa1397070-mRNA-1
tig0022126 CDS 27669 27755 27669 ID=Msa1397070-mRNA-1:cds;Parent=Msa1397070-mRNA-1
tig0022126 CDS 29436 29502 29436 ID=Msa1397070-mRNA-1:cds;Parent=Msa1397070-mRNA-1
tig0022126 CDS 29592 29842 29592 ID=Msa1397070-mRNA-1:cds;Parent=Msa1397070-mRNA-1
tig0022126 three_prime_UTR 29843 30201 29843 ID=Msa1397070-mRNA-1:three_prime_utr;Parent=Msa1397070-mRNA-1
Gene Sequence

>Msa1397070

ATGGCTTCAGATCCAAAGCTTCACACTTTCGATGATGTGTCAAAGCACAACAAGACCAAAGATTGCTGGCTTATTCTCTCTGGAAAGGTGTACGATGTCACCCCTTTTATGGAGGATCATCCCGGAGGTGATGAGGTTCTGTTGTCTGCAACAGGGAAAGATGCAACCAATGATTTTGAAGATGTGGGACACAGTGATTCTGCTAGAGAAATGATGGACAAATACTACATTGGTGAGATTGATCCATCCACTGTTCCCCTTAAGCGCACCTACGTTCCACCTCAGCAATCTCAGTACAATCCGGACAAGACTTCGGAATTTGTGATCAAGATTTTGCAGTTCCTGGTGCCTCTCCTGATATTGGGCTTAGCCTTTGTTGTCAGAAACTACACCAAGAAAGAGTAG
Protein sequence

>Msa1397070

MASDPKLHTFDDVSKHNKTKDCWLILSGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKRTYVPPQQSQYNPDKTSEFVIKILQFLVPLLILGLAFVVRNYTKKE*