AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0471850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0471850 MtrunA17_Chr3g0127451 98.507 134 2 0 1 134 1 134 4.14e-97 275
Msa0471850 MtrunA17_Chr4g0010061 66.667 132 44 0 1 132 14 145 2.14e-64 192
Msa0471850 MtrunA17_Chr8g0346311 58.088 136 54 1 1 133 1 136 5.40e-55 169
Msa0471850 MtrunA17_Chr7g0276081 56.296 135 57 1 2 134 3 137 6.90e-44 140
Msa0471850 MtrunA17_Chr5g0419651 60.714 84 33 0 1 84 1 84 1.79e-34 116
Msa0471850 MtrunA17_Chr3g0134591 34.783 138 81 1 3 131 2 139 2.97e-26 95.9
Msa0471850 MtrunA17_Chr3g0115151 42.500 80 45 1 1 80 457 535 2.71e-18 79.7
Msa0471850 MtrunA17_Chr3g0115131 45.333 75 40 1 6 80 517 590 2.39e-17 77.0
Msa0471850 MtrunA17_Chr6g0486891 44.286 70 38 1 12 81 50 118 1.59e-16 70.5
Msa0471850 MtrunA17_Chr5g0424491 40.000 80 47 1 1 80 513 591 1.69e-16 74.7
Msa0471850 MtrunA17_Chr4g0019781 33.333 81 54 0 3 83 137 217 6.45e-12 60.5
Msa0471850 MtrunA17_Chr8g0361931 38.571 70 42 1 9 78 10 78 2.96e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0471850 AT5G53560.1 79.104 134 28 0 1 134 1 134 8.24e-78 226
Msa0471850 AT2G32720.1 66.667 132 44 0 1 132 1 132 3.84e-67 199
Msa0471850 AT5G48810.1 60.294 136 52 1 1 134 1 136 8.65e-61 184
Msa0471850 AT2G32720.2 70.270 111 33 0 22 132 22 132 2.21e-55 169
Msa0471850 AT2G46650.1 51.938 129 60 1 7 133 4 132 1.30e-38 127
Msa0471850 AT1G26340.1 44.882 127 60 2 6 127 6 127 9.71e-37 122
Msa0471850 AT1G37130.1 42.500 80 45 1 1 80 538 616 4.80e-19 82.4
Msa0471850 AT1G60660.1 44.737 76 41 1 5 80 46 120 2.71e-18 75.5
Msa0471850 AT1G77760.1 40.000 80 47 1 1 80 541 619 2.55e-17 77.4
Msa0471850 AT3G61580.1 38.158 76 46 1 3 78 5 79 5.57e-13 65.1
Msa0471850 AT2G46210.1 38.571 70 42 1 9 78 11 79 2.70e-11 60.1

Find 60 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCACAATCCAACATTATT+AGG 0.187332 3_3:-74222855 None:intergenic
TCAAATCTCTTCTTCACTTT+GGG 0.187989 3_3:-74220197 None:intergenic
CCCCATAAACAGGAAAATTA+AGG 0.220364 3_3:-74222982 None:intergenic
CCTGGTGCCTCTCCTGATAT+TGG 0.232035 3_3:+74222622 Msa0471850:CDS
GACAGTGTTTAGTTTCTTCT+TGG 0.292754 3_3:+74222755 Msa0471850:three_prime_UTR
CTGGTGCCTCTCCTGATATT+GGG 0.297310 3_3:+74222623 Msa0471850:CDS
AAGAAGGAACTACTCTTTCT+TGG 0.318516 3_3:-74222672 None:intergenic
GAGAATAAGCCAGCAATCTT+TGG 0.319930 3_3:-74220590 None:intergenic
GTTTAGCATCTTTGTTTCAT+TGG 0.320261 3_3:+74222891 Msa0471850:three_prime_UTR
GTCAAATCTCTTCTTCACTT+TGG 0.341887 3_3:-74220198 None:intergenic
GTGGAACGTAGGTGCGCTTA+AGG 0.375400 3_3:-74222538 None:intergenic
CACTTTGCATCCCCATAAAC+AGG 0.379042 3_3:-74222992 None:intergenic
GATTGCTGGCTTATTCTCTC+TGG 0.386144 3_3:+74220595 Msa0471850:CDS
CAACTTTCTGGTAATGGTAC+TGG 0.390866 3_3:+74222813 Msa0471850:three_prime_UTR
TCCCACATCTTCAAAATCAT+TGG 0.392408 3_3:-74222447 None:intergenic
GGTGTCTTGAGCATTGAAAC+TGG 0.406699 3_3:+74222776 Msa0471850:three_prime_UTR
TCATCGAAAGTGTGAAGCTT+TGG 0.412470 3_3:-74220547 None:intergenic
CAAAGGCTAAGCCCAATATC+AGG 0.426178 3_3:-74222634 None:intergenic
GGTCTGTTAGCTATTTATAG+AGG 0.431918 3_3:+74222721 Msa0471850:three_prime_UTR
CCCATAAACAGGAAAATTAA+GGG 0.432212 3_3:-74222981 None:intergenic
TCTCTTCTTCACTTTGGGGT+TGG 0.435970 3_3:-74220192 None:intergenic
CAAATCTCTTCTTCACTTTG+GGG 0.437199 3_3:-74220196 None:intergenic
AATTACCACTTCCGGTCCTT+GGG 0.440955 3_3:-74220252 None:intergenic
GAATTACCACTTCCGGTCCT+TGG 0.443825 3_3:-74220253 None:intergenic
TGGAACGTAGGTGCGCTTAA+GGG 0.449787 3_3:-74222537 None:intergenic
GAGGTTCTGTTGTCTGCAAC+AGG 0.466883 3_3:+74222324 Msa0471850:CDS
ACAACAAGACCAAAGATTGC+TGG 0.472357 3_3:+74220581 Msa0471850:CDS
CGCTTAAGGGGAACAGTGGA+TGG 0.472619 3_3:-74222524 None:intergenic
ACAAATTCCGAAGTCTTGTC+CGG 0.476643 3_3:-74222581 None:intergenic
CAAGAGGGAATTACCACTTC+CGG 0.485709 3_3:-74220260 None:intergenic
ATCATATTGCACCCTTGTGA+GGG 0.492456 3_3:+74222699 Msa0471850:three_prime_UTR
TGATTCTGCTAGAGAAATGA+TGG 0.506307 3_3:+74222475 Msa0471850:CDS
CAACAGAACCTCATCACCTC+CGG 0.513023 3_3:-74222313 None:intergenic
CTGGCTTATTCTCTCTGGAA+AGG 0.542076 3_3:+74220600 Msa0471850:CDS
CATCATATTGCACCCTTGTG+AGG 0.542934 3_3:+74222698 Msa0471850:three_prime_UTR
AATCTCCCAAGGACCGGAAG+TGG 0.548565 3_3:+74220247 Msa0471850:five_prime_UTR
CCAATATCAGGAGAGGCACC+AGG 0.552438 3_3:-74222622 None:intergenic
GGAACGTAGGTGCGCTTAAG+GGG 0.553895 3_3:-74222536 None:intergenic
TGCAACTAGATTATTGTCCC+AGG 0.555593 3_3:+74223025 Msa0471850:three_prime_UTR
GTGCAATATGATGATAAAGA+AGG 0.560027 3_3:-74222688 None:intergenic
ATTTATAGAGGGTCATTCAA+AGG 0.568496 3_3:+74222733 Msa0471850:three_prime_UTR
ACTCAGAATCTCCCAAGGAC+CGG 0.572963 3_3:+74220241 Msa0471850:five_prime_UTR
GTCTGTTAGCTATTTATAGA+GGG 0.573849 3_3:+74222722 Msa0471850:three_prime_UTR
GGATCATCCCGGAGGTGATG+AGG 0.581222 3_3:+74222305 Msa0471850:CDS
AACAGAACCTCATCACCTCC+GGG 0.588122 3_3:-74222312 None:intergenic
GTACAATCCGGACAAGACTT+CGG 0.598055 3_3:+74222574 Msa0471850:CDS
TCAGCAATCTCAGTACAATC+CGG 0.604833 3_3:+74222562 Msa0471850:CDS
GGGATGATCCTCCATAAAAG+AGG 0.608521 3_3:-74222292 None:intergenic
ATGATGGACAAATACTACAT+TGG 0.610366 3_3:+74222491 Msa0471850:CDS
TAGCTAACAGACCCCTCACA+AGG 0.620015 3_3:-74222711 None:intergenic
TTGTACTGAGATTGCTGAGG+TGG 0.630347 3_3:-74222557 None:intergenic
GGTGTAGTTTCTGACAACAA+AGG 0.643712 3_3:-74222651 None:intergenic
TCATATTGCACCCTTGTGAG+GGG 0.653808 3_3:+74222700 Msa0471850:three_prime_UTR
TCATCACTCAGAATCTCCCA+AGG 0.658723 3_3:+74220236 Msa0471850:five_prime_UTR
GCTAAGCCCAATATCAGGAG+AGG 0.665710 3_3:-74222629 None:intergenic
GGTGCGCTTAAGGGGAACAG+TGG 0.672781 3_3:-74222528 None:intergenic
AGCTAACAGACCCCTCACAA+GGG 0.690074 3_3:-74222710 None:intergenic
GGATTGTACTGAGATTGCTG+AGG 0.741733 3_3:-74222560 None:intergenic
TTTATGGAGGATCATCCCGG+AGG 0.748938 3_3:+74222297 Msa0471850:CDS
AGATTGCTGAGGTGGAACGT+AGG 0.774863 3_3:-74222549 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTAAAGAATCAATAAAAA+GGG - chr3_3:74220425-74220444 None:intergenic 15.0%
!!! ATTCAGATAAAATTAATTTG+TGG + chr3_3:74220459-74220478 Msa0471850:intron 15.0%
!!! TTCAGATAAAATTAATTTGT+GGG + chr3_3:74220460-74220479 Msa0471850:intron 15.0%
!!! TATATTACATTTGTATTTTG+TGG + chr3_3:74221579-74221598 Msa0471850:intron 15.0%
!!! ATATTACATTTGTATTTTGT+GGG + chr3_3:74221580-74221599 Msa0471850:intron 15.0%
!! ATAAATTAATAACCCATTAT+AGG - chr3_3:74221807-74221826 None:intergenic 15.0%
!! TGTAGAAAAATTTATGTATT+TGG - chr3_3:74222187-74222206 None:intergenic 15.0%
!! ATAGTACAATATAATCAATA+TGG - chr3_3:74222399-74222418 None:intergenic 15.0%
!! TCTAAAGAATCAATAAAAAG+GGG - chr3_3:74220424-74220443 None:intergenic 20.0%
!! GATCTAAAGAATCAATAAAA+AGG - chr3_3:74220426-74220445 None:intergenic 20.0%
!! CAACATTCAATCTTAAAAAT+AGG - chr3_3:74220906-74220925 None:intergenic 20.0%
!! TTGAATTATTCAAATCTGAA+CGG + chr3_3:74221192-74221211 Msa0471850:intron 20.0%
!! AGTTAAAATATTAGTACATG+TGG - chr3_3:74221433-74221452 None:intergenic 20.0%
!!! TATTACATTTGTATTTTGTG+GGG + chr3_3:74221581-74221600 Msa0471850:intron 20.0%
!!! TTATTGGTATCCTTTTAAAA+TGG + chr3_3:74221735-74221754 Msa0471850:intron 20.0%
!!! TATAATGTTTTACAACTTTC+TGG + chr3_3:74222801-74222820 Msa0471850:three_prime_UTR 20.0%
!!! AGTGATCCTTTAAAAAAAAA+AGG - chr3_3:74222929-74222948 None:intergenic 20.0%
! TTCCATGATTGATTATTCTT+CGG + chr3_3:74220650-74220669 Msa0471850:intron 25.0%
!!! AAGATGAAGTTCAAGTTTTT+GGG + chr3_3:74220753-74220772 Msa0471850:intron 25.0%
!! TTTAAGATTGAATGTTGTTG+AGG + chr3_3:74220909-74220928 Msa0471850:intron 25.0%
!!! GTAGACAGATTACATTTTTT+AGG + chr3_3:74220996-74221015 Msa0471850:intron 25.0%
! TACAATAAGATCCAAACTTA+AGG - chr3_3:74221167-74221186 None:intergenic 25.0%
!! CGATGACTCATTTTATTATT+TGG - chr3_3:74221264-74221283 None:intergenic 25.0%
! GTGTAAGAAGAATGAATATT+AGG + chr3_3:74221528-74221547 Msa0471850:intron 25.0%
! TAAGTTAATCTACCTCTTAT+TGG + chr3_3:74221719-74221738 Msa0471850:intron 25.0%
! TTTAAAAGGATACCAATAAG+AGG - chr3_3:74221734-74221753 None:intergenic 25.0%
! AAGCATCTAAGCTATATAAA+AGG - chr3_3:74221967-74221986 None:intergenic 25.0%
!! AACGTTTAAAATAGTGAAAG+TGG - chr3_3:74222072-74222091 None:intergenic 25.0%
! AAGAACAAATTATCGTTAAG+AGG - chr3_3:74222247-74222266 None:intergenic 25.0%
! ATTTGTTCTTCAATCATGTT+AGG + chr3_3:74222257-74222276 Msa0471850:intron 25.0%
! TATGCTTATATCATTCGAAA+AGG + chr3_3:74222347-74222366 Msa0471850:intron 25.0%
! ATGCTTATATCATTCGAAAA+GGG + chr3_3:74222348-74222367 Msa0471850:intron 25.0%
! TACTATACTGTATCAATTAC+AGG + chr3_3:74222413-74222432 Msa0471850:intron 25.0%
! ACTATACTGTATCAATTACA+GGG + chr3_3:74222414-74222433 Msa0471850:intron 25.0%
! ACTGAAAAACCTAATAATGT+TGG + chr3_3:74222846-74222865 Msa0471850:three_prime_UTR 25.0%
! ATTCACAATCCAACATTATT+AGG - chr3_3:74222858-74222877 None:intergenic 25.0%
!!! CTCAAGCCTTTTTTTTTTAA+AGG + chr3_3:74222920-74222939 Msa0471850:three_prime_UTR 25.0%
TCAAATCTCTTCTTCACTTT+GGG - chr3_3:74220200-74220219 None:intergenic 30.0%
TAGAAAAGAATCGATGCAAA+GGG - chr3_3:74220313-74220332 None:intergenic 30.0%
TTAGAAAAGAATCGATGCAA+AGG - chr3_3:74220314-74220333 None:intergenic 30.0%
!!! GGTTTGACTTGAATTTTTCT+GGG + chr3_3:74220481-74220500 Msa0471850:intron 30.0%
TGGCAGTGAAAGAAAAAAAA+TGG + chr3_3:74220516-74220535 Msa0471850:intron 30.0%
TTCCGAAGAATAATCAATCA+TGG - chr3_3:74220655-74220674 None:intergenic 30.0%
!! GAAGATGAAGTTCAAGTTTT+TGG + chr3_3:74220752-74220771 Msa0471850:intron 30.0%
TGCATGTTACATATATTTCC+TGG + chr3_3:74220780-74220799 Msa0471850:intron 30.0%
TCAGTGTGGTTAACAAATTA+TGG + chr3_3:74220954-74220973 Msa0471850:intron 30.0%
AATAAGATCCAAACTTAAGG+TGG - chr3_3:74221164-74221183 None:intergenic 30.0%
ATTCATTCTTCTTACACGTT+CGG - chr3_3:74221525-74221544 None:intergenic 30.0%
!!! TTGGTATCCTTTTAAAATGG+AGG + chr3_3:74221738-74221757 Msa0471850:intron 30.0%
!!! TGGTATCCTTTTAAAATGGA+GGG + chr3_3:74221739-74221758 Msa0471850:intron 30.0%
AATAACCCATTATAGGAAGA+CGG - chr3_3:74221800-74221819 None:intergenic 30.0%
TAAAGAATCAAACTGTGGAA+AGG - chr3_3:74221843-74221862 None:intergenic 30.0%
AAGGATAAAGAATCAAACTG+TGG - chr3_3:74221848-74221867 None:intergenic 30.0%
GCTATATAAAAGGAAGATTG+AGG - chr3_3:74221957-74221976 None:intergenic 30.0%
TATAGCTTAGATGCTTTCAT+TGG + chr3_3:74221971-74221990 Msa0471850:intron 30.0%
TCTTATAGGAAGATAGGTTA+GGG - chr3_3:74222016-74222035 None:intergenic 30.0%
CTAATGTCTTATAGGAAGAT+AGG - chr3_3:74222022-74222041 None:intergenic 30.0%
AATTAGAATAGCCTCACAAT+TGG - chr3_3:74222112-74222131 None:intergenic 30.0%
!! AACCAATGATTTTGAAGATG+TGG + chr3_3:74222445-74222464 Msa0471850:CDS 30.0%
!! ACCAATGATTTTGAAGATGT+GGG + chr3_3:74222446-74222465 Msa0471850:CDS 30.0%
!! ATGATGGACAAATACTACAT+TGG + chr3_3:74222491-74222510 Msa0471850:CDS 30.0%
GTGCAATATGATGATAAAGA+AGG - chr3_3:74222691-74222710 None:intergenic 30.0%
GTCTGTTAGCTATTTATAGA+GGG + chr3_3:74222722-74222741 Msa0471850:three_prime_UTR 30.0%
ATTTATAGAGGGTCATTCAA+AGG + chr3_3:74222733-74222752 Msa0471850:three_prime_UTR 30.0%
!! GTTTTACAACTTTCTGGTAA+TGG + chr3_3:74222807-74222826 Msa0471850:three_prime_UTR 30.0%
! GTTTAGCATCTTTGTTTCAT+TGG + chr3_3:74222891-74222910 Msa0471850:three_prime_UTR 30.0%
! ACCCTTAATTTTCCTGTTTA+TGG + chr3_3:74222980-74222999 Msa0471850:three_prime_UTR 30.0%
CCCATAAACAGGAAAATTAA+GGG - chr3_3:74222984-74223003 None:intergenic 30.0%
! CCCTTAATTTTCCTGTTTAT+GGG + chr3_3:74222981-74223000 Msa0471850:three_prime_UTR 30.0%
! CCTTAATTTTCCTGTTTATG+GGG + chr3_3:74222982-74223001 Msa0471850:three_prime_UTR 30.0%
CAAATCTCTTCTTCACTTTG+GGG - chr3_3:74220199-74220218 None:intergenic 35.0%
GTCAAATCTCTTCTTCACTT+TGG - chr3_3:74220201-74220220 None:intergenic 35.0%
TTGGAAGGGAAAAAACAAGA+GGG - chr3_3:74220278-74220297 None:intergenic 35.0%
ATTGGAAGGGAAAAAACAAG+AGG - chr3_3:74220279-74220298 None:intergenic 35.0%
GGAAAAGAGAAGAATTGGAA+GGG - chr3_3:74220292-74220311 None:intergenic 35.0%
CAAAGGGAAAAGAGAAGAAT+TGG - chr3_3:74220297-74220316 None:intergenic 35.0%
!!! GGGTTTGACTTGAATTTTTC+TGG + chr3_3:74220480-74220499 Msa0471850:intron 35.0%
!! TTTCTGGGTCTTTGATTTTG+TGG + chr3_3:74220496-74220515 Msa0471850:intron 35.0%
TTTGGTCATCTATGAGTAAG+AGG - chr3_3:74221246-74221265 None:intergenic 35.0%
CATGCAAAATTACTGGTGTT+AGG - chr3_3:74221306-74221325 None:intergenic 35.0%
TCTCTAGCATGCAAAATTAC+TGG - chr3_3:74221313-74221332 None:intergenic 35.0%
! TAATTTTGCATGCTAGAGAG+TGG + chr3_3:74221314-74221333 Msa0471850:intron 35.0%
GAGTAGAAGAATATAGCATC+TGG + chr3_3:74221339-74221358 Msa0471850:intron 35.0%
ACGACTAGTAATGCACATAA+AGG - chr3_3:74221396-74221415 None:intergenic 35.0%
TTCATTCTTCTTACACGTTC+GGG - chr3_3:74221524-74221543 None:intergenic 35.0%
!!! TATCCTTTTAAAATGGAGGG+AGG + chr3_3:74221742-74221761 Msa0471850:intron 35.0%
! TTTCCTCCCTCCATTTTAAA+AGG - chr3_3:74221748-74221767 None:intergenic 35.0%
AAAATGGAGGGAGGAAAAAA+AGG + chr3_3:74221751-74221770 Msa0471850:intron 35.0%
ATAAACCCGTCTTCCTATAA+TGG + chr3_3:74221791-74221810 Msa0471850:intron 35.0%
TAAACCCGTCTTCCTATAAT+GGG + chr3_3:74221792-74221811 Msa0471850:intron 35.0%
ATAACCCATTATAGGAAGAC+GGG - chr3_3:74221799-74221818 None:intergenic 35.0%
! TTAGTCAAATATGCTCTGAG+AGG + chr3_3:74221924-74221943 Msa0471850:intron 35.0%
CTTATAGGAAGATAGGTTAG+GGG - chr3_3:74222015-74222034 None:intergenic 35.0%
GTCTTATAGGAAGATAGGTT+AGG - chr3_3:74222017-74222036 None:intergenic 35.0%
! TCGACTAGCTAATGTCTTAT+AGG - chr3_3:74222030-74222049 None:intergenic 35.0%
TCCCACATCTTCAAAATCAT+TGG - chr3_3:74222450-74222469 None:intergenic 35.0%
TGATTCTGCTAGAGAAATGA+TGG + chr3_3:74222475-74222494 Msa0471850:CDS 35.0%
AAGAAGGAACTACTCTTTCT+TGG - chr3_3:74222675-74222694 None:intergenic 35.0%
GGTCTGTTAGCTATTTATAG+AGG + chr3_3:74222721-74222740 Msa0471850:three_prime_UTR 35.0%
! GACAGTGTTTAGTTTCTTCT+TGG + chr3_3:74222755-74222774 Msa0471850:three_prime_UTR 35.0%
CCCCATAAACAGGAAAATTA+AGG - chr3_3:74222985-74223004 None:intergenic 35.0%
GGGAAAAGAGAAGAATTGGA+AGG - chr3_3:74220293-74220312 None:intergenic 40.0%
! TCATCGAAAGTGTGAAGCTT+TGG - chr3_3:74220550-74220569 None:intergenic 40.0%
ACAACAAGACCAAAGATTGC+TGG + chr3_3:74220581-74220600 Msa0471850:CDS 40.0%
GAGAATAAGCCAGCAATCTT+TGG - chr3_3:74220593-74220612 None:intergenic 40.0%
ATGCTATGTCTAGATCAGTG+TGG + chr3_3:74220940-74220959 Msa0471850:intron 40.0%
GTCTACAAGTACAGAAGAAC+AGG - chr3_3:74220982-74221001 None:intergenic 40.0%
ACAAACGAAGCATGTCATGT+AGG - chr3_3:74221135-74221154 None:intergenic 40.0%
AAGATCCAAACTTAAGGTGG+TGG - chr3_3:74221161-74221180 None:intergenic 40.0%
AGATAGATCTCAAGATCTGC+AGG - chr3_3:74221661-74221680 None:intergenic 40.0%
ATCTGATGTTTGATGCCATG+TGG + chr3_3:74221677-74221696 Msa0471850:intron 40.0%
TCTGATGTTTGATGCCATGT+GGG + chr3_3:74221678-74221697 Msa0471850:intron 40.0%
! ATAGGAAGACGGGTTTATGA+AGG - chr3_3:74221789-74221808 None:intergenic 40.0%
AACTGTGGAAAGGAAGAGTA+TGG - chr3_3:74221833-74221852 None:intergenic 40.0%
CTAGTCGAAAATCTAGCAGA+AGG + chr3_3:74222042-74222061 Msa0471850:intron 40.0%
! ATGGTGCTCTGCATTTTTCA+GGG - chr3_3:74222135-74222154 None:intergenic 40.0%
! ATGCAGAGCACCATGTATAT+TGG + chr3_3:74222141-74222160 Msa0471850:intron 40.0%
! ACCATGTATATTGGCCTGAA+TGG + chr3_3:74222150-74222169 Msa0471850:intron 40.0%
ACCATTCAGGCCAATATACA+TGG - chr3_3:74222154-74222173 None:intergenic 40.0%
! GTACGATGTCACCTCTTTTA+TGG + chr3_3:74222281-74222300 Msa0471850:CDS 40.0%
! TCTTTTATGGAGGATCATCC+CGG + chr3_3:74222294-74222313 Msa0471850:CDS 40.0%
TCAGCAATCTCAGTACAATC+CGG + chr3_3:74222562-74222581 Msa0471850:CDS 40.0%
! ACAAATTCCGAAGTCTTGTC+CGG - chr3_3:74222584-74222603 None:intergenic 40.0%
! GATCAAGATTTTGCAGTTCC+TGG + chr3_3:74222604-74222623 Msa0471850:CDS 40.0%
GGTGTAGTTTCTGACAACAA+AGG - chr3_3:74222654-74222673 None:intergenic 40.0%
ATCATATTGCACCCTTGTGA+GGG + chr3_3:74222699-74222718 Msa0471850:three_prime_UTR 40.0%
!! CAACTTTCTGGTAATGGTAC+TGG + chr3_3:74222813-74222832 Msa0471850:three_prime_UTR 40.0%
!! TCTCTTCTTCACTTTGGGGT+TGG - chr3_3:74220195-74220214 None:intergenic 45.0%
TCATCACTCAGAATCTCCCA+AGG + chr3_3:74220236-74220255 Msa0471850:five_prime_UTR 45.0%
AATTACCACTTCCGGTCCTT+GGG - chr3_3:74220255-74220274 None:intergenic 45.0%
CAAGAGGGAATTACCACTTC+CGG - chr3_3:74220263-74220282 None:intergenic 45.0%
!! GATTGCTGGCTTATTCTCTC+TGG + chr3_3:74220595-74220614 Msa0471850:CDS 45.0%
! CTGGCTTATTCTCTCTGGAA+AGG + chr3_3:74220600-74220619 Msa0471850:CDS 45.0%
ACATGCAATGCACAAGATCC+AGG - chr3_3:74220801-74220820 None:intergenic 45.0%
GTCAACCACCACCTTAAGTT+TGG + chr3_3:74221153-74221172 Msa0471850:intron 45.0%
GTGCATTACTAGTCGTGTAC+TGG + chr3_3:74221400-74221419 Msa0471850:intron 45.0%
CTGCTCGCTTATGAAAGGTA+TGG - chr3_3:74221471-74221490 None:intergenic 45.0%
CGATACTGCTCGCTTATGAA+AGG - chr3_3:74221476-74221495 None:intergenic 45.0%
TTTGATGCCATGTGGGACTT+GGG + chr3_3:74221685-74221704 Msa0471850:intron 45.0%
CATTGTGTTTCGACGTCACA+GGG - chr3_3:74221876-74221895 None:intergenic 45.0%
CTGTGACGTCGAAACACAAT+GGG + chr3_3:74221875-74221894 Msa0471850:intron 45.0%
TGTGACGTCGAAACACAATG+GGG + chr3_3:74221876-74221895 Msa0471850:intron 45.0%
! AAACACAATGGGGAGAGTGT+TGG + chr3_3:74221886-74221905 Msa0471850:intron 45.0%
!! GTCAAATATGCTCTGAGAGG+TGG + chr3_3:74221927-74221946 Msa0471850:intron 45.0%
TAGGAAGATAGGTTAGGGGA+AGG - chr3_3:74222011-74222030 None:intergenic 45.0%
AACGTTGCCTGATATTGCGA+TGG + chr3_3:74222086-74222105 Msa0471850:intron 45.0%
CAATTGGCCATCGCAATATC+AGG - chr3_3:74222096-74222115 None:intergenic 45.0%
TATTGCGATGGCCAATTGTG+AGG + chr3_3:74222098-74222117 Msa0471850:intron 45.0%
! CATGGTGCTCTGCATTTTTC+AGG - chr3_3:74222136-74222155 None:intergenic 45.0%
TTGGCCTGAATGGTCACTAT+TGG + chr3_3:74222160-74222179 Msa0471850:intron 45.0%
TGGCCTGAATGGTCACTATT+GGG + chr3_3:74222161-74222180 Msa0471850:intron 45.0%
! CGATGTCACCTCTTTTATGG+AGG + chr3_3:74222284-74222303 Msa0471850:CDS 45.0%
GGGATGATCCTCCATAAAAG+AGG - chr3_3:74222295-74222314 None:intergenic 45.0%
!! GGTATGCTACTAAAGCACTG+AGG + chr3_3:74222369-74222388 Msa0471850:intron 45.0%
TTGTACTGAGATTGCTGAGG+TGG - chr3_3:74222560-74222579 None:intergenic 45.0%
GGATTGTACTGAGATTGCTG+AGG - chr3_3:74222563-74222582 None:intergenic 45.0%
GTACAATCCGGACAAGACTT+CGG + chr3_3:74222574-74222593 Msa0471850:CDS 45.0%
CAAAGGCTAAGCCCAATATC+AGG - chr3_3:74222637-74222656 None:intergenic 45.0%
CATCATATTGCACCCTTGTG+AGG + chr3_3:74222698-74222717 Msa0471850:three_prime_UTR 45.0%
TCATATTGCACCCTTGTGAG+GGG + chr3_3:74222700-74222719 Msa0471850:three_prime_UTR 45.0%
!! GGTGTCTTGAGCATTGAAAC+TGG + chr3_3:74222776-74222795 Msa0471850:three_prime_UTR 45.0%
CACTTTGCATCCCCATAAAC+AGG - chr3_3:74222995-74223014 None:intergenic 45.0%
ACTCAGAATCTCCCAAGGAC+CGG + chr3_3:74220241-74220260 Msa0471850:five_prime_UTR 50.0%
GAATTACCACTTCCGGTCCT+TGG - chr3_3:74220256-74220275 None:intergenic 50.0%
GTTTGATGCCATGTGGGACT+TGG + chr3_3:74221684-74221703 Msa0471850:intron 50.0%
CCATTGTGTTTCGACGTCAC+AGG - chr3_3:74221877-74221896 None:intergenic 50.0%
CCTGTGACGTCGAAACACAA+TGG + chr3_3:74221874-74221893 Msa0471850:intron 50.0%
TGGCCCAATAGTGACCATTC+AGG - chr3_3:74222167-74222186 None:intergenic 50.0%
TTTATGGAGGATCATCCCGG+AGG + chr3_3:74222297-74222316 Msa0471850:CDS 50.0%
AACAGAACCTCATCACCTCC+GGG - chr3_3:74222315-74222334 None:intergenic 50.0%
CAACAGAACCTCATCACCTC+CGG - chr3_3:74222316-74222335 None:intergenic 50.0%
GAGGTTCTGTTGTCTGCAAC+AGG + chr3_3:74222324-74222343 Msa0471850:CDS 50.0%
TGGAACGTAGGTGCGCTTAA+GGG - chr3_3:74222540-74222559 None:intergenic 50.0%
AGATTGCTGAGGTGGAACGT+AGG - chr3_3:74222552-74222571 None:intergenic 50.0%
CTGGTGCCTCTCCTGATATT+GGG + chr3_3:74222623-74222642 Msa0471850:CDS 50.0%
GCTAAGCCCAATATCAGGAG+AGG - chr3_3:74222632-74222651 None:intergenic 50.0%
AGCTAACAGACCCCTCACAA+GGG - chr3_3:74222713-74222732 None:intergenic 50.0%
TAGCTAACAGACCCCTCACA+AGG - chr3_3:74222714-74222733 None:intergenic 50.0%
AATCTCCCAAGGACCGGAAG+TGG + chr3_3:74220247-74220266 Msa0471850:five_prime_UTR 55.0%
ATGCCATGTGGGACTTGGGA+AGG + chr3_3:74221689-74221708 Msa0471850:intron 55.0%
TGCCATGTGGGACTTGGGAA+GGG + chr3_3:74221690-74221709 Msa0471850:intron 55.0%
TTCCCTTCCCAAGTCCCACA+TGG - chr3_3:74221695-74221714 None:intergenic 55.0%
TCGACGTCACAGGGAACAGA+AGG - chr3_3:74221867-74221886 None:intergenic 55.0%
CGCTTAAGGGGAACAGTGGA+TGG - chr3_3:74222527-74222546 None:intergenic 55.0%
GGAACGTAGGTGCGCTTAAG+GGG - chr3_3:74222539-74222558 None:intergenic 55.0%
GTGGAACGTAGGTGCGCTTA+AGG - chr3_3:74222541-74222560 None:intergenic 55.0%
!! CCAATATCAGGAGAGGCACC+AGG - chr3_3:74222625-74222644 None:intergenic 55.0%
CCTGGTGCCTCTCCTGATAT+TGG + chr3_3:74222622-74222641 Msa0471850:CDS 55.0%
GGATCATCCCGGAGGTGATG+AGG + chr3_3:74222305-74222324 Msa0471850:CDS 60.0%
GGTGCGCTTAAGGGGAACAG+TGG - chr3_3:74222531-74222550 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 74220191 74223044 74220191 ID=Msa0471850;Name=Msa0471850
chr3_3 mRNA 74220191 74223044 74220191 ID=Msa0471850-mRNA-1;Parent=Msa0471850;Name=Msa0471850-mRNA-1;_AED=0.01;_eAED=0.01;_QI=91|1|1|1|0.66|0.5|4|359|134
chr3_3 exon 74220191 74220268 74220191 ID=Msa0471850-mRNA-1:exon:18927;Parent=Msa0471850-mRNA-1
chr3_3 exon 74220522 74220621 74220522 ID=Msa0471850-mRNA-1:exon:18928;Parent=Msa0471850-mRNA-1
chr3_3 exon 74222279 74222345 74222279 ID=Msa0471850-mRNA-1:exon:18929;Parent=Msa0471850-mRNA-1
chr3_3 exon 74222435 74223044 74222435 ID=Msa0471850-mRNA-1:exon:18930;Parent=Msa0471850-mRNA-1
chr3_3 five_prime_UTR 74220191 74220268 74220191 ID=Msa0471850-mRNA-1:five_prime_utr;Parent=Msa0471850-mRNA-1
chr3_3 five_prime_UTR 74220522 74220534 74220522 ID=Msa0471850-mRNA-1:five_prime_utr;Parent=Msa0471850-mRNA-1
chr3_3 CDS 74220535 74220621 74220535 ID=Msa0471850-mRNA-1:cds;Parent=Msa0471850-mRNA-1
chr3_3 CDS 74222279 74222345 74222279 ID=Msa0471850-mRNA-1:cds;Parent=Msa0471850-mRNA-1
chr3_3 CDS 74222435 74222685 74222435 ID=Msa0471850-mRNA-1:cds;Parent=Msa0471850-mRNA-1
chr3_3 three_prime_UTR 74222686 74223044 74222686 ID=Msa0471850-mRNA-1:three_prime_utr;Parent=Msa0471850-mRNA-1
Gene Sequence

>Msa0471850

ATGGCTTCAGATCCAAAGCTTCACACTTTCGATGATGTGTCAAAGCACAACAAGACCAAAGATTGCTGGCTTATTCTCTCTGGAAAGGTGTACGATGTCACCTCTTTTATGGAGGATCATCCCGGAGGTGATGAGGTTCTGTTGTCTGCAACAGGGAAAGATGCAACCAATGATTTTGAAGATGTGGGACACAGTGATTCTGCTAGAGAAATGATGGACAAATACTACATTGGTGAGATTGATCCATCCACTGTTCCCCTTAAGCGCACCTACGTTCCACCTCAGCAATCTCAGTACAATCCGGACAAGACTTCGGAATTTGTGATCAAGATTTTGCAGTTCCTGGTGCCTCTCCTGATATTGGGCTTAGCCTTTGTTGTCAGAAACTACACCAAGAAAGAGTAG
Protein sequence

>Msa0471850

MASDPKLHTFDDVSKHNKTKDCWLILSGKVYDVTSFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKRTYVPPQQSQYNPDKTSEFVIKILQFLVPLLILGLAFVVRNYTKKE*