AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0444040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0444040 MtrunA17_Chr1g0183691 89.474 95 10 0 1 95 53 147 1.19e-58 183
Msa0444040 MtrunA17_Chr2g0325501 47.826 92 44 2 1 89 66 156 9.35e-22 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0444040 AT1G03900.1 72.340 94 26 0 1 94 55 148 6.59e-44 145
Msa0444040 AT3G58600.1 45.652 92 46 2 1 89 75 165 3.46e-20 84.3

Find 97 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGTTTAATTGACAATATT+TGG 0.125399 3_3:-16773291 None:intergenic
ATTAAAACTTAAGAATTAAT+TGG 0.153202 3_3:+16776320 Msa0444040:three_prime_UTR
AACGGAATGGGTTAAAATTA+TGG 0.195683 3_3:-16773466 None:intergenic
AAAATGGGGTTTCATGGTTT+TGG 0.235392 3_3:-16773341 None:intergenic
AACAAGGGTGGTAGAGATTT+AGG 0.237128 3_3:-16773396 None:intergenic
CAAGGGAAGCATGTGTTTAT+TGG 0.237220 3_3:+16773840 Msa0444040:CDS
TTTGTAGACCTTCAAGTTAT+AGG 0.264756 3_3:+16776352 Msa0444040:three_prime_UTR
AAGCATGTGTTTATTGGATT+AGG 0.266751 3_3:+16773846 Msa0444040:CDS
TATTTCTTAGTTGGTACATT+TGG 0.267786 3_3:+16774367 Msa0444040:intron
ACTCTTCACATTAATGCTTT+TGG 0.278094 3_3:+16773268 None:intergenic
AGTGAGATTGAGGAATAAAA+TGG 0.312064 3_3:-16773357 None:intergenic
AGGAATAAAATGGGGTTTCA+TGG 0.315510 3_3:-16773347 None:intergenic
CAAGTTATAGGTTATCCTAT+TGG 0.318712 3_3:+16776364 Msa0444040:three_prime_UTR
ATTTCTTAGTTGGTACATTT+GGG 0.325548 3_3:+16774368 Msa0444040:intron
AGCATGTGTTTATTGGATTA+GGG 0.332772 3_3:+16773847 Msa0444040:CDS
CTTGTCCTATATATATGTTT+TGG 0.337556 3_3:-16773571 None:intergenic
TTCTTCCAAAACATATATAT+AGG 0.349435 3_3:+16773566 Msa0444040:five_prime_UTR
CTTCTAAAGATTGTGTATAA+TGG 0.357548 3_3:+16776116 Msa0444040:three_prime_UTR
GAGCAACTGTAGAAAAGAAA+TGG 0.357579 3_3:-16773535 None:intergenic
AGAAGCTAATGACTAACTTA+AGG 0.365596 3_3:+16775944 Msa0444040:three_prime_UTR
TTAAAACTTAAGAATTAATT+GGG 0.366320 3_3:+16776321 Msa0444040:three_prime_UTR
TGTGGGAACGAGACTGAAAT+TGG 0.369214 3_3:-16773315 None:intergenic
GGTGAGAGCTTAAATGAAAC+TGG 0.376751 3_3:-16773444 None:intergenic
AATACCTATGACTTGCGTTT+TGG 0.377633 3_3:-16775118 None:intergenic
GAGATTTAGGTTTCAATTTG+AGG 0.394504 3_3:-16773383 None:intergenic
ACGGAATGGGTTAAAATTAT+GGG 0.402922 3_3:-16773465 None:intergenic
AGTATTATCAATCATCCAAT+AGG 0.414360 3_3:-16776379 None:intergenic
TGCTGTTAATCATAGGCTTA+AGG 0.419472 3_3:+16773976 Msa0444040:CDS
GTGAGATTGAGGAATAAAAT+GGG 0.426659 3_3:-16773356 None:intergenic
GAAGCTAATGACTAACTTAA+GGG 0.429396 3_3:+16775945 Msa0444040:three_prime_UTR
AACTGAACCCTCACACTGAC+CGG 0.431437 3_3:-16773766 None:intergenic
TGTGAGAAGAGAACATGATA+AGG 0.442504 3_3:+16773929 Msa0444040:CDS
GCTTAAATGAAACTGGATAA+AGG 0.443636 3_3:-16773437 None:intergenic
AGCGAAGAGATCGCCGGAGT+TGG 0.447638 3_3:-16773727 None:intergenic
GTGTGAGGGTTCAGTTGAAC+TGG 0.448765 3_3:+16773773 Msa0444040:CDS
TAGGATAACCTATAACTTGA+AGG 0.448867 3_3:-16776360 None:intergenic
ACTGCCAAAACGCAAGTCAT+AGG 0.449501 3_3:+16775114 Msa0444040:CDS
AGATTTAGGTTTCAATTTGA+GGG 0.451063 3_3:-16773382 None:intergenic
TTTCTGCTTAAGATTGAAGA+TGG 0.451141 3_3:+16773816 Msa0444040:CDS
GCGAAGAGATCGCCGGAGTT+GGG 0.452093 3_3:-16773726 None:intergenic
GTTTATTGGATTAGGGTTAA+CGG 0.452324 3_3:+16773854 Msa0444040:CDS
CGCCGGAGGTTGTTCGTGGC+GGG 0.452665 3_3:-16773605 None:intergenic
AAAGGCATGTGTTACGTCAA+TGG 0.457053 3_3:+16776065 Msa0444040:three_prime_UTR
GCGTTATGTCCCGCCGTGAC+CGG 0.461427 3_3:+16773682 Msa0444040:exon
GCCATCCTGCTGTTAATCAT+AGG 0.463255 3_3:+16773969 Msa0444040:CDS
GGTTCCAATCAGACAAGATC+TGG 0.463783 3_3:+16773646 Msa0444040:five_prime_UTR
AGAGAACATGATAAGGAGTC+TGG 0.464167 3_3:+16773936 Msa0444040:CDS
GCCGTGACCGGTGTGAGATA+CGG 0.467324 3_3:+16773694 Msa0444040:CDS
TTTGAGGGAAAGTGAGATTG+AGG 0.478972 3_3:-16773367 None:intergenic
ATCCCGCCACGAACAACCTC+CGG 0.483393 3_3:+16773603 Msa0444040:five_prime_UTR
TTAGGCAAATCAACAACTGC+TGG 0.486080 3_3:+16776251 Msa0444040:intron
TAACCGCCGGAGGTTGTTCG+TGG 0.487228 3_3:-16773609 None:intergenic
CGGACCAGATCTTGTCTGAT+TGG 0.492781 3_3:-16773650 None:intergenic
TTTCAATTTGAATTGTACTC+TGG 0.494796 3_3:+16776447 Msa0444040:three_prime_UTR
GGACATAACGCGGAGACGGC+CGG 0.497460 3_3:-16773670 None:intergenic
GCGGTTACAAATGCGGCTAA+TGG 0.502661 3_3:+16773625 Msa0444040:five_prime_UTR
AGAAAAGAAATGGGTCGAAA+AGG 0.503425 3_3:-16773525 None:intergenic
CAATCAGACAAGATCTGGTC+CGG 0.506501 3_3:+16773651 Msa0444040:five_prime_UTR
GATTAACAGCAGGATGGCAT+TGG 0.514115 3_3:-16773964 None:intergenic
AGCAACTGTAGAAAAGAAAT+GGG 0.514548 3_3:-16773534 None:intergenic
AGTGAAGAACGGTGGAGAAA+CGG 0.515942 3_3:-16773484 None:intergenic
AGATTTAGCCTGTCGAGTGA+TGG 0.527799 3_3:+16775085 Msa0444040:intron
ACCGTATCTCACACCGGTCA+CGG 0.531370 3_3:-16773695 None:intergenic
CATAATCCTTCGATTGAACA+AGG 0.536572 3_3:-16773412 None:intergenic
TATCTCACACCGGTCACGGC+GGG 0.540870 3_3:-16773691 None:intergenic
GTAATGTTCCATCACTCGAC+AGG 0.545474 3_3:-16775093 None:intergenic
ACAGGAGTTGAAGTAGAGAA+AGG 0.547321 3_3:+16775896 Msa0444040:intron
ACTGAACCCTCACACTGACC+GGG 0.558637 3_3:-16773765 None:intergenic
TACCACCCTTGTTCAATCGA+AGG 0.561434 3_3:+16773406 Msa0444040:five_prime_UTR
GGCGGGACATAACGCGGAGA+CGG 0.565853 3_3:-16773674 None:intergenic
GAACGGTGGAGAAACGGAAT+GGG 0.566786 3_3:-16773478 None:intergenic
CCGCCGGAGGTTGTTCGTGG+CGG 0.571890 3_3:-16773606 None:intergenic
CTGTCTTACATCACATCTCA+AGG 0.573407 3_3:+16774430 Msa0444040:CDS
CTTAAGATTGAAGATGGACA+AGG 0.579822 3_3:+16773822 Msa0444040:CDS
TCAACAACTGCTGGTGTACG+TGG 0.580588 3_3:+16776260 Msa0444040:three_prime_UTR
AGAACGGTGGAGAAACGGAA+TGG 0.592306 3_3:-16773479 None:intergenic
TTGGATTAGGGTTAACGGAG+AGG 0.594029 3_3:+16773859 Msa0444040:CDS
TTAAGCCTATGATTAACAGC+AGG 0.600310 3_3:-16773974 None:intergenic
GTAAGACAGTTGAAGAAGTG+TGG 0.607868 3_3:-16774416 None:intergenic
TGAGATTGAGGAATAAAATG+GGG 0.611914 3_3:-16773355 None:intergenic
GCCGCATTTGTAACCGCCGG+AGG 0.612310 3_3:-16773619 None:intergenic
ACCTCCGGCGGTTACAAATG+CGG 0.616884 3_3:+16773618 Msa0444040:five_prime_UTR
TTAGCCGCATTTGTAACCGC+CGG 0.625611 3_3:-16773622 None:intergenic
ACACGCAGCGAAGAGATCGC+CGG 0.626051 3_3:-16773733 None:intergenic
ATCCTTCGATTGAACAAGGG+TGG 0.639676 3_3:-16773408 None:intergenic
GCCTATGATTAACAGCAGGA+TGG 0.640269 3_3:-16773970 None:intergenic
TTCAGCACTGCTAAGACTCG+AGG 0.650870 3_3:+16776292 Msa0444040:three_prime_UTR
CGGTTAGAAGATCCCAACTC+CGG 0.656754 3_3:+16773714 Msa0444040:CDS
TTAAGATTGAAGATGGACAA+GGG 0.676442 3_3:+16773823 Msa0444040:CDS
GGTCACGGCGGGACATAACG+CGG 0.678044 3_3:-16773680 None:intergenic
CAACAAAGAGTGAAGAACGG+TGG 0.678077 3_3:-16773492 None:intergenic
CTTCTAACCGTATCTCACAC+CGG 0.687609 3_3:-16773701 None:intergenic
TGGATAACGACAACACTCGA+CGG 0.689843 3_3:-16774396 None:intergenic
GAACAACAAAGAGTGAAGAA+CGG 0.690550 3_3:-16773495 None:intergenic
CCGCCACGAACAACCTCCGG+CGG 0.697916 3_3:+16773606 Msa0444040:five_prime_UTR
ATAATCCTTCGATTGAACAA+GGG 0.716702 3_3:-16773411 None:intergenic
GTATCTCACACCGGTCACGG+CGG 0.748302 3_3:-16773692 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAACTTAAGAATTAAT+TGG + chr3_3:16776320-16776339 Msa0444040:three_prime_UTR 10.0%
!! TTAAAACTTAAGAATTAATT+GGG + chr3_3:16776321-16776340 Msa0444040:three_prime_UTR 10.0%
!! AAAGTTTAATTGACAATATT+TGG - chr3_3:16773294-16773313 None:intergenic 15.0%
!!! ACTATTTTTCAACTAAAAAT+TGG - chr3_3:16774065-16774084 None:intergenic 15.0%
!! AACTTAAACTTTGTAAAAAT+AGG + chr3_3:16774158-16774177 Msa0444040:intron 15.0%
!! TACAATTAGAAAATAGAATA+AGG - chr3_3:16774755-16774774 None:intergenic 15.0%
!! TTATAAAAAAATGAGACATA+AGG + chr3_3:16774813-16774832 Msa0444040:intron 15.0%
!! TATAAAAAAATGAGACATAA+GGG + chr3_3:16774814-16774833 Msa0444040:intron 15.0%
!! AGTAAAACTAAAAAATAAGA+TGG - chr3_3:16775752-16775771 None:intergenic 15.0%
!!! TATATATGTTTTGGAAGAAA+AGG - chr3_3:16773565-16773584 None:intergenic 20.0%
!! TTCTTCCAAAACATATATAT+AGG + chr3_3:16773566-16773585 Msa0444040:five_prime_UTR 20.0%
!!! CTTTATTTCGAATTTGTTAA+TGG + chr3_3:16775770-16775789 Msa0444040:intron 20.0%
!!! AACAATTTTTATGCCATTAA+CGG + chr3_3:16776013-16776032 Msa0444040:intron 20.0%
!!! TGATTTTGTACAAAAAAACA+AGG - chr3_3:16776216-16776235 None:intergenic 20.0%
!!! GTTAGATTTTATGTTTTTGA+TGG + chr3_3:16776412-16776431 Msa0444040:three_prime_UTR 20.0%
!! AGATTTAGGTTTCAATTTGA+GGG - chr3_3:16773385-16773404 None:intergenic 25.0%
!! CTTGTCCTATATATATGTTT+TGG - chr3_3:16773574-16773593 None:intergenic 25.0%
!! TATGCATAAAATTTGTTTCC+AGG + chr3_3:16774122-16774141 Msa0444040:intron 25.0%
!! ATGCATAAAATTTGTTTCCA+GGG + chr3_3:16774123-16774142 Msa0444040:intron 25.0%
!! TATTTCTTAGTTGGTACATT+TGG + chr3_3:16774367-16774386 Msa0444040:intron 25.0%
!! ATTTCTTAGTTGGTACATTT+GGG + chr3_3:16774368-16774387 Msa0444040:intron 25.0%
! TGTTACTATAGTTGAATACA+AGG + chr3_3:16774473-16774492 Msa0444040:intron 25.0%
! TCTTAAAAAGACAACACAAA+TGG + chr3_3:16774553-16774572 Msa0444040:intron 25.0%
! CTAATATGTGAATAATCAAG+CGG + chr3_3:16774578-16774597 Msa0444040:intron 25.0%
!! TAATATGACTTGTGAAATGA+AGG + chr3_3:16774780-16774799 Msa0444040:intron 25.0%
!! AATATGACTTGTGAAATGAA+GGG + chr3_3:16774781-16774800 Msa0444040:intron 25.0%
!! ACATTTTACTGAAGAAATTC+AGG - chr3_3:16775432-16775451 None:intergenic 25.0%
!!! TATATATGCTTTGTTTTGAC+AGG - chr3_3:16775520-16775539 None:intergenic 25.0%
! CAAAGCATATATATACGTAT+TGG + chr3_3:16775527-16775546 Msa0444040:intron 25.0%
! CTGAAATTAGAGTTCAAAAA+GGG + chr3_3:16775818-16775837 Msa0444040:intron 25.0%
!! CTTCTAAAGATTGTGTATAA+TGG + chr3_3:16776116-16776135 Msa0444040:three_prime_UTR 25.0%
!! TTTTGTACAAAATCAAACCT+TGG + chr3_3:16776221-16776240 Msa0444040:intron 25.0%
! AGTATTATCAATCATCCAAT+AGG - chr3_3:16776382-16776401 None:intergenic 25.0%
!!! TATGTTTTTGATGGAACTAT+TGG + chr3_3:16776421-16776440 Msa0444040:three_prime_UTR 25.0%
! TTTCAATTTGAATTGTACTC+TGG + chr3_3:16776447-16776466 Msa0444040:three_prime_UTR 25.0%
TGAGATTGAGGAATAAAATG+GGG - chr3_3:16773358-16773377 None:intergenic 30.0%
GTGAGATTGAGGAATAAAAT+GGG - chr3_3:16773359-16773378 None:intergenic 30.0%
AGTGAGATTGAGGAATAAAA+TGG - chr3_3:16773360-16773379 None:intergenic 30.0%
! GAGATTTAGGTTTCAATTTG+AGG - chr3_3:16773386-16773405 None:intergenic 30.0%
ATAATCCTTCGATTGAACAA+GGG - chr3_3:16773414-16773433 None:intergenic 30.0%
GCTTAAATGAAACTGGATAA+AGG - chr3_3:16773440-16773459 None:intergenic 30.0%
!! ACGGAATGGGTTAAAATTAT+GGG - chr3_3:16773468-16773487 None:intergenic 30.0%
!! AACGGAATGGGTTAAAATTA+TGG - chr3_3:16773469-16773488 None:intergenic 30.0%
AGCAACTGTAGAAAAGAAAT+GGG - chr3_3:16773537-16773556 None:intergenic 30.0%
TTTCTGCTTAAGATTGAAGA+TGG + chr3_3:16773816-16773835 Msa0444040:CDS 30.0%
TTAAGATTGAAGATGGACAA+GGG + chr3_3:16773823-16773842 Msa0444040:CDS 30.0%
AAGCATGTGTTTATTGGATT+AGG + chr3_3:16773846-16773865 Msa0444040:CDS 30.0%
AGCATGTGTTTATTGGATTA+GGG + chr3_3:16773847-16773866 Msa0444040:CDS 30.0%
! GTTTATTGGATTAGGGTTAA+CGG + chr3_3:16773854-16773873 Msa0444040:CDS 30.0%
! TCTCATGTTTGTGAACTTTT+AGG + chr3_3:16774215-16774234 Msa0444040:intron 30.0%
TTGCTGTGATATTTCTTAGT+TGG + chr3_3:16774358-16774377 Msa0444040:intron 30.0%
AGTAACACATGTAAAGAGAA+GGG - chr3_3:16774460-16774479 None:intergenic 30.0%
TAGTAACACATGTAAAGAGA+AGG - chr3_3:16774461-16774480 None:intergenic 30.0%
TATAGTTGAATACAAGGTCA+TGG + chr3_3:16774479-16774498 Msa0444040:intron 30.0%
GTTGCAAGCTATAAACTATA+AGG + chr3_3:16774965-16774984 Msa0444040:intron 30.0%
CAGAAAATTGAATGAACAGA+CGG - chr3_3:16775054-16775073 None:intergenic 30.0%
TTATAATACGTACTCTGTGT+CGG - chr3_3:16775232-16775251 None:intergenic 30.0%
CAATTTCTTCTGTCAATATC+TGG + chr3_3:16775324-16775343 Msa0444040:intron 30.0%
AACTAATGAAGTTACCTCTT+GGG - chr3_3:16775695-16775714 None:intergenic 30.0%
AAACTAATGAAGTTACCTCT+TGG - chr3_3:16775696-16775715 None:intergenic 30.0%
AAGAGGTAACTTCATTAGTT+TGG + chr3_3:16775695-16775714 Msa0444040:intron 30.0%
GCTGAAATTAGAGTTCAAAA+AGG + chr3_3:16775817-16775836 Msa0444040:intron 30.0%
AGAAGCTAATGACTAACTTA+AGG + chr3_3:16775944-16775963 Msa0444040:three_prime_UTR 30.0%
GAAGCTAATGACTAACTTAA+GGG + chr3_3:16775945-16775964 Msa0444040:three_prime_UTR 30.0%
TCTGCTTTGATAGAGAAAAA+AGG + chr3_3:16776047-16776066 Msa0444040:intron 30.0%
TCAAACCTTGGATATTTCTT+AGG + chr3_3:16776233-16776252 Msa0444040:intron 30.0%
ATTTGCCTAAGAAATATCCA+AGG - chr3_3:16776241-16776260 None:intergenic 30.0%
! TTTGTAGACCTTCAAGTTAT+AGG + chr3_3:16776352-16776371 Msa0444040:three_prime_UTR 30.0%
TAGGATAACCTATAACTTGA+AGG - chr3_3:16776363-16776382 None:intergenic 30.0%
CAAGTTATAGGTTATCCTAT+TGG + chr3_3:16776364-16776383 Msa0444040:three_prime_UTR 30.0%
!!! TTTCATGGTTTTGGAGTTGT+GGG - chr3_3:16773335-16773354 None:intergenic 35.0%
! AAAATGGGGTTTCATGGTTT+TGG - chr3_3:16773344-16773363 None:intergenic 35.0%
AGGAATAAAATGGGGTTTCA+TGG - chr3_3:16773350-16773369 None:intergenic 35.0%
CATAATCCTTCGATTGAACA+AGG - chr3_3:16773415-16773434 None:intergenic 35.0%
GAACAACAAAGAGTGAAGAA+CGG - chr3_3:16773498-16773517 None:intergenic 35.0%
AGAAAAGAAATGGGTCGAAA+AGG - chr3_3:16773528-16773547 None:intergenic 35.0%
GAGCAACTGTAGAAAAGAAA+TGG - chr3_3:16773538-16773557 None:intergenic 35.0%
CTTAAGATTGAAGATGGACA+AGG + chr3_3:16773822-16773841 Msa0444040:CDS 35.0%
TGTGAGAAGAGAACATGATA+AGG + chr3_3:16773929-16773948 Msa0444040:CDS 35.0%
TTAAGCCTATGATTAACAGC+AGG - chr3_3:16773977-16773996 None:intergenic 35.0%
TGCTGTTAATCATAGGCTTA+AGG + chr3_3:16773976-16773995 Msa0444040:CDS 35.0%
! ATAGACATTGGTTAGACACA+TGG - chr3_3:16774191-16774210 None:intergenic 35.0%
ACATGAGACAGTATAGACAT+TGG - chr3_3:16774203-16774222 None:intergenic 35.0%
AGACATAAGGGCTAAAAGAA+AGG + chr3_3:16774826-16774845 Msa0444040:intron 35.0%
TGATATGCATGTTAAGAACG+AGG - chr3_3:16774876-16774895 None:intergenic 35.0%
CAACACATATATCTTTCCTC+AGG - chr3_3:16774938-16774957 None:intergenic 35.0%
!! AATACCTATGACTTGCGTTT+TGG - chr3_3:16775121-16775140 None:intergenic 35.0%
TCATATGCACATTCAGAAAG+AGG - chr3_3:16775161-16775180 None:intergenic 35.0%
ATTGACACACATTCGTAAAG+AGG - chr3_3:16775308-16775327 None:intergenic 35.0%
TTCTTCTGTCAATATCTGGA+TGG + chr3_3:16775328-16775347 Msa0444040:intron 35.0%
! GTCTTTTCTGATATGTTCTG+CGG + chr3_3:16775350-16775369 Msa0444040:intron 35.0%
!!! TTTTAGAACCTACGCTTCAA+TGG - chr3_3:16775402-16775421 None:intergenic 35.0%
TGATATGCTCATTCAGATAG+AGG - chr3_3:16775457-16775476 None:intergenic 35.0%
AGCTCTCTTGAAATAGTGTT+AGG + chr3_3:16775667-16775686 Msa0444040:intron 35.0%
! AGGTAACTTCATTAGTTTGG+TGG + chr3_3:16775698-16775717 Msa0444040:intron 35.0%
AAAAGGGCTATGATATCATC+AGG + chr3_3:16775834-16775853 Msa0444040:intron 35.0%
AAAGGGCTATGATATCATCA+GGG + chr3_3:16775835-16775854 Msa0444040:intron 35.0%
!!! GTTTCATGGTTTTGGAGTTG+TGG - chr3_3:16773336-16773355 None:intergenic 40.0%
TTTGAGGGAAAGTGAGATTG+AGG - chr3_3:16773370-16773389 None:intergenic 40.0%
AACAAGGGTGGTAGAGATTT+AGG - chr3_3:16773399-16773418 None:intergenic 40.0%
GGTGAGAGCTTAAATGAAAC+TGG - chr3_3:16773447-16773466 None:intergenic 40.0%
! CAAGGGAAGCATGTGTTTAT+TGG + chr3_3:16773840-16773859 Msa0444040:CDS 40.0%
AGAGAACATGATAAGGAGTC+TGG + chr3_3:16773936-16773955 Msa0444040:CDS 40.0%
!! TTTGTTTCCAGGGTACTGTT+AGG + chr3_3:16774133-16774152 Msa0444040:intron 40.0%
GTAAGACAGTTGAAGAAGTG+TGG - chr3_3:16774419-16774438 None:intergenic 40.0%
CTGTCTTACATCACATCTCA+AGG + chr3_3:16774430-16774449 Msa0444040:CDS 40.0%
TGAATACAAGGTCATGGCAT+TGG + chr3_3:16774485-16774504 Msa0444040:intron 40.0%
TTTCAACCAAATGAGCTCAC+AGG - chr3_3:16774514-16774533 None:intergenic 40.0%
CTCATGAAAAAACAGACCAG+AGG - chr3_3:16774616-16774635 None:intergenic 40.0%
ATTCCTTCTAATGCAACCTG+AGG + chr3_3:16774919-16774938 Msa0444040:intron 40.0%
! TTTCCTCAGGTTGCATTAGA+AGG - chr3_3:16774925-16774944 None:intergenic 40.0%
CTCTGATTATCTTTAGCGAC+AGG + chr3_3:16775197-16775216 Msa0444040:intron 40.0%
TTGCAAAACCATTGAAGCGT+AGG + chr3_3:16775391-16775410 Msa0444040:intron 40.0%
AATAGTGTTAGGAACCCAAG+AGG + chr3_3:16775678-16775697 Msa0444040:intron 40.0%
TGATATCATCAGGGTGAGTT+TGG + chr3_3:16775844-16775863 Msa0444040:intron 40.0%
! CTAGTTGTTCTTTTGTCGAC+AGG + chr3_3:16775878-16775897 Msa0444040:intron 40.0%
ACAGGAGTTGAAGTAGAGAA+AGG + chr3_3:16775896-16775915 Msa0444040:intron 40.0%
GATGATGCTTTCACCGTTAA+TGG - chr3_3:16776029-16776048 None:intergenic 40.0%
! AAAGGCATGTGTTACGTCAA+TGG + chr3_3:16776065-16776084 Msa0444040:three_prime_UTR 40.0%
TGATAGCTTGACATTGCCTT+TGG + chr3_3:16776165-16776184 Msa0444040:intron 40.0%
GTCTTAAACGTGTACACCAA+AGG - chr3_3:16776184-16776203 None:intergenic 40.0%
TTAGGCAAATCAACAACTGC+TGG + chr3_3:16776251-16776270 Msa0444040:intron 40.0%
TGTGGGAACGAGACTGAAAT+TGG - chr3_3:16773318-16773337 None:intergenic 45.0%
TACCACCCTTGTTCAATCGA+AGG + chr3_3:16773406-16773425 Msa0444040:five_prime_UTR 45.0%
ATCCTTCGATTGAACAAGGG+TGG - chr3_3:16773411-16773430 None:intergenic 45.0%
AGTGAAGAACGGTGGAGAAA+CGG - chr3_3:16773487-16773506 None:intergenic 45.0%
CAACAAAGAGTGAAGAACGG+TGG - chr3_3:16773495-16773514 None:intergenic 45.0%
GGTTCCAATCAGACAAGATC+TGG + chr3_3:16773646-16773665 Msa0444040:five_prime_UTR 45.0%
CAATCAGACAAGATCTGGTC+CGG + chr3_3:16773651-16773670 Msa0444040:five_prime_UTR 45.0%
CTTCTAACCGTATCTCACAC+CGG - chr3_3:16773704-16773723 None:intergenic 45.0%
!!! GCTGCGTGTTTTGTTTTTCC+CGG + chr3_3:16773747-16773766 Msa0444040:CDS 45.0%
! TTGGATTAGGGTTAACGGAG+AGG + chr3_3:16773859-16773878 Msa0444040:CDS 45.0%
GATTAACAGCAGGATGGCAT+TGG - chr3_3:16773967-16773986 None:intergenic 45.0%
GCCATCCTGCTGTTAATCAT+AGG + chr3_3:16773969-16773988 Msa0444040:CDS 45.0%
GCCTATGATTAACAGCAGGA+TGG - chr3_3:16773973-16773992 None:intergenic 45.0%
AAGTTTGCCTAACAGTACCC+TGG - chr3_3:16774143-16774162 None:intergenic 45.0%
TGGATAACGACAACACTCGA+CGG - chr3_3:16774399-16774418 None:intergenic 45.0%
GCGGCATTACATATTACCTC+TGG + chr3_3:16774597-16774616 Msa0444040:intron 45.0%
AGATTTAGCCTGTCGAGTGA+TGG + chr3_3:16775085-16775104 Msa0444040:intron 45.0%
GTAATGTTCCATCACTCGAC+AGG - chr3_3:16775096-16775115 None:intergenic 45.0%
ACTGCCAAAACGCAAGTCAT+AGG + chr3_3:16775114-16775133 Msa0444040:CDS 45.0%
!!! ACTTTAAAATAATATTTTAT+TGG + chr3_3:16774664-16774683 Msa0444040:intron 5.0%
!!! TTTTTAACAATATTATATTT+TGG - chr3_3:16775576-16775595 None:intergenic 5.0%
GAACGGTGGAGAAACGGAAT+GGG - chr3_3:16773481-16773500 None:intergenic 50.0%
AGAACGGTGGAGAAACGGAA+TGG - chr3_3:16773482-16773501 None:intergenic 50.0%
TTAGCCGCATTTGTAACCGC+CGG - chr3_3:16773625-16773644 None:intergenic 50.0%
GCGGTTACAAATGCGGCTAA+TGG + chr3_3:16773625-16773644 Msa0444040:five_prime_UTR 50.0%
CGGACCAGATCTTGTCTGAT+TGG - chr3_3:16773653-16773672 None:intergenic 50.0%
CGGTTAGAAGATCCCAACTC+CGG + chr3_3:16773714-16773733 Msa0444040:CDS 50.0%
! TGTTTTTCCCGGTCAGTGTG+AGG + chr3_3:16773758-16773777 Msa0444040:CDS 50.0%
! GTTTTTCCCGGTCAGTGTGA+GGG + chr3_3:16773759-16773778 Msa0444040:CDS 50.0%
AACTGAACCCTCACACTGAC+CGG - chr3_3:16773769-16773788 None:intergenic 50.0%
GTGTGAGGGTTCAGTTGAAC+TGG + chr3_3:16773773-16773792 Msa0444040:CDS 50.0%
TGGTGACCTGTGAGCTCATT+TGG + chr3_3:16774505-16774524 Msa0444040:intron 50.0%
GACGGACATGCATGTGCAAA+AGG - chr3_3:16775036-16775055 None:intergenic 50.0%
! CGTACTCTGTGTCGGTACAA+AGG - chr3_3:16775224-16775243 None:intergenic 50.0%
TCAACAACTGCTGGTGTACG+TGG + chr3_3:16776260-16776279 Msa0444040:three_prime_UTR 50.0%
TTCAGCACTGCTAAGACTCG+AGG + chr3_3:16776292-16776311 Msa0444040:three_prime_UTR 50.0%
ACCTCCGGCGGTTACAAATG+CGG + chr3_3:16773618-16773637 Msa0444040:five_prime_UTR 55.0%
ACCGTATCTCACACCGGTCA+CGG - chr3_3:16773698-16773717 None:intergenic 55.0%
ACTGAACCCTCACACTGACC+GGG - chr3_3:16773768-16773787 None:intergenic 55.0%
ATCCCGCCACGAACAACCTC+CGG + chr3_3:16773603-16773622 Msa0444040:five_prime_UTR 60.0%
TAACCGCCGGAGGTTGTTCG+TGG - chr3_3:16773612-16773631 None:intergenic 60.0%
TATCTCACACCGGTCACGGC+GGG - chr3_3:16773694-16773713 None:intergenic 60.0%
GTATCTCACACCGGTCACGG+CGG - chr3_3:16773695-16773714 None:intergenic 60.0%
GCCGTGACCGGTGTGAGATA+CGG + chr3_3:16773694-16773713 Msa0444040:CDS 60.0%
GCGAAGAGATCGCCGGAGTT+GGG - chr3_3:16773729-16773748 None:intergenic 60.0%
AGCGAAGAGATCGCCGGAGT+TGG - chr3_3:16773730-16773749 None:intergenic 60.0%
ACACGCAGCGAAGAGATCGC+CGG - chr3_3:16773736-16773755 None:intergenic 60.0%
GCCGCATTTGTAACCGCCGG+AGG - chr3_3:16773622-16773641 None:intergenic 65.0%
GGACATAACGCGGAGACGGC+CGG - chr3_3:16773673-16773692 None:intergenic 65.0%
GGCGGGACATAACGCGGAGA+CGG - chr3_3:16773677-16773696 None:intergenic 65.0%
GGTCACGGCGGGACATAACG+CGG - chr3_3:16773683-16773702 None:intergenic 65.0%
GCGTTATGTCCCGCCGTGAC+CGG + chr3_3:16773682-16773701 Msa0444040:exon 65.0%
CGCCGGAGGTTGTTCGTGGC+GGG - chr3_3:16773608-16773627 None:intergenic 70.0%
CCGCCGGAGGTTGTTCGTGG+CGG - chr3_3:16773609-16773628 None:intergenic 70.0%
CCGCCACGAACAACCTCCGG+CGG + chr3_3:16773606-16773625 Msa0444040:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 16773277 16776536 16773277 ID=Msa0444040;Name=Msa0444040
chr3_3 mRNA 16773277 16776536 16773277 ID=Msa0444040-mRNA-1;Parent=Msa0444040;Name=Msa0444040-mRNA-1;_AED=0.26;_eAED=0.26;_QI=410|1|1|1|0.4|0.5|6|427|144
chr3_3 exon 16773277 16773997 16773277 ID=Msa0444040-mRNA-1:exon:3466;Parent=Msa0444040-mRNA-1
chr3_3 exon 16774377 16774451 16774377 ID=Msa0444040-mRNA-1:exon:3467;Parent=Msa0444040-mRNA-1
chr3_3 exon 16775087 16775135 16775087 ID=Msa0444040-mRNA-1:exon:3468;Parent=Msa0444040-mRNA-1
chr3_3 exon 16775900 16775966 16775900 ID=Msa0444040-mRNA-1:exon:3469;Parent=Msa0444040-mRNA-1
chr3_3 exon 16776060 16776137 16776060 ID=Msa0444040-mRNA-1:exon:3470;Parent=Msa0444040-mRNA-1
chr3_3 exon 16776255 16776536 16776255 ID=Msa0444040-mRNA-1:exon:3471;Parent=Msa0444040-mRNA-1
chr3_3 five_prime_UTR 16773277 16773686 16773277 ID=Msa0444040-mRNA-1:five_prime_utr;Parent=Msa0444040-mRNA-1
chr3_3 CDS 16773687 16773997 16773687 ID=Msa0444040-mRNA-1:cds;Parent=Msa0444040-mRNA-1
chr3_3 CDS 16774377 16774451 16774377 ID=Msa0444040-mRNA-1:cds;Parent=Msa0444040-mRNA-1
chr3_3 CDS 16775087 16775135 16775087 ID=Msa0444040-mRNA-1:cds;Parent=Msa0444040-mRNA-1
chr3_3 three_prime_UTR 16775900 16775966 16775900 ID=Msa0444040-mRNA-1:three_prime_utr;Parent=Msa0444040-mRNA-1
chr3_3 three_prime_UTR 16776060 16776137 16776060 ID=Msa0444040-mRNA-1:three_prime_utr;Parent=Msa0444040-mRNA-1
chr3_3 three_prime_UTR 16776255 16776536 16776255 ID=Msa0444040-mRNA-1:three_prime_utr;Parent=Msa0444040-mRNA-1
Gene Sequence

>Msa0444040

ATGTCCCGCCGTGACCGGTGTGAGATACGGTTAGAAGATCCCAACTCCGGCGATCTCTTCGCTGCGTGTTTTGTTTTTCCCGGTCAGTGTGAGGGTTCAGTTGAACTGGTTGTCGATTCATCGAGATACTTTCTGCTTAAGATTGAAGATGGACAAGGGAAGCATGTGTTTATTGGATTAGGGTTAACGGAGAGGAGTGAAGCTTTTGATTTCAATGTTGCGCTTTCTGATCATGAGAAGTATGTGAGAAGAGAACATGATAAGGAGTCTGGTGATGCCAATGCCATCCTGCTGTTAATCATAGGCTTAAGTTGGTACATTTGGGATTTGCCGTCGAGTGTTGTCGTTATCCACACTTCTTCAACTGTCTTACATCACATCTCAAGATTTAGCCTGTCGAGTGATGGAACATTACTGCCAAAACGCAAGTCATAG
Protein sequence

>Msa0444040

MSRRDRCEIRLEDPNSGDLFAACFVFPGQCEGSVELVVDSSRYFLLKIEDGQGKHVFIGLGLTERSEAFDFNVALSDHEKYVRREHDKESGDANAILLLIIGLSWYIWDLPSSVVVIHTSSTVLHHISRFSLSSDGTLLPKRKS*