AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1448020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1448020 MtrunA17_Chr6g0458911 90.385 104 10 0 32 135 79 182 7.20e-64 192
Msa1448020 MtrunA17_Chr6g0458981 75.962 104 25 0 32 135 71 174 2.81e-54 170
Msa1448020 MtrunA17_Chr4g0064241 74.286 105 26 1 32 135 65 169 8.09e-50 157
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1448020 AT3G27310.1 70.874 103 30 0 32 134 79 181 6.56e-49 157

Find 100 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTTCACATTAATGCTTT+TGG 0.207367 tig0031953:+525 None:intergenic
GATGATGCTTTCACCGTTAA+TGG 0.208999 tig0031953:-3284 None:intergenic
CACAAATAAAATGGCTTATC+TGG 0.217366 tig0031953:-4972 None:intergenic
AAAATGGGGTTTCATGGTTT+TGG 0.235392 tig0031953:-598 None:intergenic
AACAAGGGTGGTAGAGATTT+AGG 0.237128 tig0031953:-653 None:intergenic
CAAGGGAAGCATGTGTTTAT+TGG 0.237220 tig0031953:+1097 Msa1448020:five_prime_UTR
TCGGCTTCTCTGAGTTTCTT+AGG 0.258760 tig0031953:-4411 None:intergenic
AAGCATGTGTTTATTGGATT+AGG 0.266751 tig0031953:+1103 Msa1448020:five_prime_UTR
TATTTCTTAGTTGGTACATT+TGG 0.267786 tig0031953:+1625 Msa1448020:intron
AAATCTTTGATCAGCTTCTT+AGG 0.272525 tig0031953:-5256 None:intergenic
GCTTCCAATATGTGATTATC+AGG 0.290264 tig0031953:-4885 None:intergenic
AGTGAGATTGAGGAATAAAA+TGG 0.312064 tig0031953:-614 None:intergenic
AGGAATAAAATGGGGTTTCA+TGG 0.315510 tig0031953:-604 None:intergenic
AACGGAATGGGTTAAAACTA+TGG 0.321523 tig0031953:-723 None:intergenic
TGTAGGAACGAGACTGAAAT+TGG 0.323514 tig0031953:-572 None:intergenic
ATTTCTTAGTTGGTACATTT+GGG 0.325548 tig0031953:+1626 Msa1448020:intron
AAATGCTTACACAAATAAAA+TGG 0.326046 tig0031953:-4981 None:intergenic
AGCATGTGTTTATTGGATTA+GGG 0.332772 tig0031953:+1104 Msa1448020:five_prime_UTR
CTTGTCCTATACATATGTTT+TGG 0.349132 tig0031953:-828 None:intergenic
TTTCCCTGATAATCACATAT+TGG 0.354531 tig0031953:+4881 Msa1448020:CDS
CTTCTAAAGATTGTGTATAA+TGG 0.357548 tig0031953:+3374 Msa1448020:CDS
GAGCAACTGTAGAAAAGAAA+TGG 0.357579 tig0031953:-792 None:intergenic
TTCTTCCAAAACATATGTAT+AGG 0.370057 tig0031953:+823 Msa1448020:five_prime_UTR
GGTGAGAGCTTAAATGAAAC+TGG 0.376751 tig0031953:-701 None:intergenic
GAGATTTAGGTTTCAATTTG+AGG 0.394504 tig0031953:-640 None:intergenic
AGAAGCTAATGACCAACTTA+AGG 0.395849 tig0031953:+3202 Msa1448020:five_prime_UTR
TGCTGTTAATCATAGGCTTA+AGG 0.419472 tig0031953:+1233 Msa1448020:five_prime_UTR
AATAAAATGGCTTATCTGGT+TGG 0.421434 tig0031953:-4968 None:intergenic
AACTGAACCCTCACACTGAC+AGG 0.423043 tig0031953:-1023 None:intergenic
GTGAGATTGAGGAATAAAAT+GGG 0.426659 tig0031953:-613 None:intergenic
TGTGAGAAGAGAACATGATA+AGG 0.442504 tig0031953:+1186 Msa1448020:five_prime_UTR
GAAGCTAATGACCAACTTAA+GGG 0.443177 tig0031953:+3203 Msa1448020:five_prime_UTR
GCTTAAATGAAACTGGATAA+AGG 0.443636 tig0031953:-694 None:intergenic
ATAAAATGGCTTATCTGGTT+GGG 0.446590 tig0031953:-4967 None:intergenic
GCGTTGTGTCCCGCCGTGAC+CGG 0.447886 tig0031953:+939 Msa1448020:five_prime_UTR
GTGTGAGGGTTCAGTTGAAC+TGG 0.448765 tig0031953:+1030 Msa1448020:five_prime_UTR
AGATTTAGGTTTCAATTTGA+GGG 0.451063 tig0031953:-639 None:intergenic
TTTCTGCTTAAGATTGAAGA+TGG 0.451141 tig0031953:+1073 Msa1448020:five_prime_UTR
GTTTATTGGATTAGGGTTAA+CGG 0.452324 tig0031953:+1111 Msa1448020:five_prime_UTR
CGCCGGAGGTTGTTCGTGGC+GGG 0.452665 tig0031953:-862 None:intergenic
AAAGGCATGTGTTACGTCAA+TGG 0.457053 tig0031953:+3323 Msa1448020:CDS
AGCGAAGAGATCGCCGAAGT+TGG 0.459540 tig0031953:-984 None:intergenic
GCCATCCTGCTGTTAATCAT+AGG 0.463255 tig0031953:+1226 Msa1448020:five_prime_UTR
GGTTCCAATCAGACAAGATC+TGG 0.463783 tig0031953:+903 Msa1448020:five_prime_UTR
AGAGAACATGATAAGGAGTC+TGG 0.464167 tig0031953:+1193 Msa1448020:five_prime_UTR
GCGAAGAGATCGCCGAAGTT+GGG 0.467204 tig0031953:-983 None:intergenic
GCCGTGACCGGTGTGAGATA+CGG 0.467324 tig0031953:+951 Msa1448020:five_prime_UTR
GGACACAACGCGGAGACGGC+CGG 0.477578 tig0031953:-927 None:intergenic
TTTGAGGGAAAGTGAGATTG+AGG 0.478972 tig0031953:-624 None:intergenic
ATCCCGCCACGAACAACCTC+CGG 0.483393 tig0031953:+860 Msa1448020:five_prime_UTR
TAACCGCCGGAGGTTGTTCG+TGG 0.487228 tig0031953:-866 None:intergenic
CGGACCAGATCTTGTCTGAT+TGG 0.492781 tig0031953:-907 None:intergenic
TAAAGTCATTACCCTTAAGT+TGG 0.492910 tig0031953:-3214 None:intergenic
AGTGAAGAACTGTGGAGAAA+CGG 0.493197 tig0031953:-741 None:intergenic
GCGGTTACAAATGCGGCTAA+TGG 0.502661 tig0031953:+882 Msa1448020:five_prime_UTR
AGAAAAGAAATGGGTCGAAA+AGG 0.503425 tig0031953:-782 None:intergenic
CAATCAGACAAGATCTGGTC+CGG 0.506501 tig0031953:+908 Msa1448020:five_prime_UTR
GGCGGGACACAACGCGGAGA+CGG 0.509066 tig0031953:-931 None:intergenic
GATTAACAGCAGGATGGCAT+TGG 0.514115 tig0031953:-1221 None:intergenic
AGCAACTGTAGAAAAGAAAT+GGG 0.514548 tig0031953:-791 None:intergenic
AAACTCTTGATTGTATCTGA+TGG 0.524164 tig0031953:-4918 None:intergenic
ACCGTATCTCACACCGGTCA+CGG 0.531370 tig0031953:-952 None:intergenic
TCGCAGGTCAAGAATAATCA+AGG 0.535076 tig0031953:+4443 Msa1448020:CDS
CATAATCCTTCGATTGAACA+AGG 0.536572 tig0031953:-669 None:intergenic
TATCTCACACCGGTCACGGC+GGG 0.540870 tig0031953:-948 None:intergenic
ACAGGAGTTGAAGTAGAGAA+AGG 0.547321 tig0031953:+3154 Msa1448020:intron
ACGGAATGGGTTAAAACTAT+GGG 0.553854 tig0031953:-722 None:intergenic
TACCACCCTTGTTCAATCGA+AGG 0.561434 tig0031953:+663 Msa1448020:five_prime_UTR
CCGCCGGAGGTTGTTCGTGG+CGG 0.571890 tig0031953:-863 None:intergenic
CTGTCTTACATCACATCTCA+AGG 0.573407 tig0031953:+1688 Msa1448020:five_prime_UTR
AAGCCGAAGTGGCATCTCGC+AGG 0.573796 tig0031953:+4427 Msa1448020:CDS
CTTAAGATTGAAGATGGACA+AGG 0.579822 tig0031953:+1079 Msa1448020:five_prime_UTR
GAACTGTGGAGAAACGGAAT+GGG 0.584247 tig0031953:-735 None:intergenic
TCTTTGATCAGCTTCTTAGG+TGG 0.591766 tig0031953:-5253 None:intergenic
AGAACTGTGGAGAAACGGAA+TGG 0.592306 tig0031953:-736 None:intergenic
TTGGATTAGGGTTAACGGAG+AGG 0.594029 tig0031953:+1116 Msa1448020:five_prime_UTR
TTAAGCCTATGATTAACAGC+AGG 0.600310 tig0031953:-1231 None:intergenic
TGACCTGCGAGATGCCACTT+CGG 0.602812 tig0031953:-4430 None:intergenic
GATAGGTACACAATGGCACC+AGG 0.603086 tig0031953:-5310 None:intergenic
CTTCCAATATGTGATTATCA+GGG 0.610149 tig0031953:-4884 None:intergenic
TGAGATTGAGGAATAAAATG+GGG 0.611914 tig0031953:-612 None:intergenic
GCCGCATTTGTAACCGCCGG+AGG 0.612310 tig0031953:-876 None:intergenic
ACCTCCGGCGGTTACAAATG+CGG 0.616884 tig0031953:+875 Msa1448020:five_prime_UTR
ACTGAACCCTCACACTGACA+GGG 0.621101 tig0031953:-1022 None:intergenic
TTAGCCGCATTTGTAACCGC+CGG 0.625611 tig0031953:-879 None:intergenic
GAAACTCAGAGAAGCCGAAG+TGG 0.630330 tig0031953:+4416 Msa1448020:CDS
GATTGTATCTGATGGATGAA+AGG 0.635886 tig0031953:-4910 None:intergenic
ATCCTTCGATTGAACAAGGG+TGG 0.639676 tig0031953:-665 None:intergenic
GCCTATGATTAACAGCAGGA+TGG 0.640269 tig0031953:-1227 None:intergenic
CGGTTAGAAGATCCCAACTT+CGG 0.643782 tig0031953:+971 Msa1448020:five_prime_UTR
CAACAAAGAGTGAAGAACTG+TGG 0.649585 tig0031953:-749 None:intergenic
GTAAGACAGTTGAAGAAGTG+TGG 0.654301 tig0031953:-1674 None:intergenic
TTAAGATTGAAGATGGACAA+GGG 0.676442 tig0031953:+1080 Msa1448020:five_prime_UTR
TGGACAACGACAACACTCGA+CGG 0.686164 tig0031953:-1654 None:intergenic
CTTCTAACCGTATCTCACAC+CGG 0.687609 tig0031953:-958 None:intergenic
TCAATATGATAGGTACACAA+TGG 0.696198 tig0031953:-5317 None:intergenic
CCGCCACGAACAACCTCCGG+CGG 0.697916 tig0031953:+863 Msa1448020:five_prime_UTR
GGTCACGGCGGGACACAACG+CGG 0.706808 tig0031953:-937 None:intergenic
ATAATCCTTCGATTGAACAA+GGG 0.716702 tig0031953:-668 None:intergenic
GTATCTCACACCGGTCACGG+CGG 0.748302 tig0031953:-949 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAACTTAAGAATTAAT+TGG + tig0031953:3582-3601 Msa1448020:intron 10.0%
!! TTAAAACTTAAGAATTAATT+GGG + tig0031953:3583-3602 Msa1448020:intron 10.0%
!!! TTTTTTTTAAATTATTTGCT+GGG - tig0031953:3897-3916 None:intergenic 10.0%
!!! TTTTTTTTTAAATTATTTGC+TGG - tig0031953:3898-3917 None:intergenic 10.0%
!! AAATAAAAAGATAAAAAGTT+TGG - tig0031953:4824-4843 None:intergenic 10.0%
!!! AAACTTTTTATCTTTTTATT+TGG + tig0031953:4823-4842 Msa1448020:intron 10.0%
!!! ACTATTTTTCAACTAAAAAT+TGG - tig0031953:1322-1341 None:intergenic 15.0%
!! AACTTAAACTTTGTAAAAAT+AGG + tig0031953:1415-1434 Msa1448020:intron 15.0%
!! TACAATTAGAAAATTGAATA+AGG - tig0031953:2013-2032 None:intergenic 15.0%
!! TTATAAAAAAATGAGACATA+AGG + tig0031953:2071-2090 Msa1448020:intron 15.0%
!! TATAAAAAAATGAGACATAA+GGG + tig0031953:2072-2091 Msa1448020:intron 15.0%
!! AGTAAAACTAAAAAATAAGA+TGG - tig0031953:3010-3029 None:intergenic 15.0%
!! TTATTTACAATATTCTTCTA+CGG - tig0031953:3795-3814 None:intergenic 15.0%
!!! ATATTCCAACTTTTAAATAT+TGG + tig0031953:4228-4247 Msa1448020:intron 15.0%
!! TCAAAAAAAAAAAAGAGAAT+CGG - tig0031953:4311-4330 None:intergenic 15.0%
!!! TTAAATTTTCACAAATACTA+AGG + tig0031953:4469-4488 Msa1448020:intron 15.0%
!!! TAAATTTTCACAAATACTAA+GGG + tig0031953:4470-4489 Msa1448020:intron 15.0%
!! TCAAAGTTTATTCTTTATAA+GGG - tig0031953:5012-5031 None:intergenic 15.0%
!! TTCAAAGTTTATTCTTTATA+AGG - tig0031953:5013-5032 None:intergenic 15.0%
!!! CTTTATTTCGAATTTGTTAA+TGG + tig0031953:3028-3047 Msa1448020:intron 20.0%
!!! AACAATTTTTATGCCATTAA+CGG + tig0031953:3271-3290 Msa1448020:exon 20.0%
!!! ATTTTGTACAAAAAAACACA+AGG - tig0031953:3476-3495 None:intergenic 20.0%
!!! GTTAGATTTTATGTTTTTGA+TGG + tig0031953:3674-3693 Msa1448020:intron 20.0%
!!! AAAAATATTTTAACCCAATC+TGG + tig0031953:3916-3935 Msa1448020:intron 20.0%
!!! AAAATATTTTAACCCAATCT+GGG + tig0031953:3917-3936 Msa1448020:intron 20.0%
!!! TACAAGAAAAACATTGATTT+GGG - tig0031953:4204-4223 None:intergenic 20.0%
!! TTAAATAACAATGTCATGAA+TGG - tig0031953:4362-4381 None:intergenic 20.0%
!!! AAATTTTCACAAATACTAAG+GGG + tig0031953:4471-4490 Msa1448020:intron 20.0%
!! AAATGCTTACACAAATAAAA+TGG - tig0031953:4984-5003 None:intergenic 20.0%
!! TAGTATCTACATTAAAAGAA+AGG - tig0031953:5231-5250 None:intergenic 20.0%
!! AGATTTAGGTTTCAATTTGA+GGG - tig0031953:642-661 None:intergenic 25.0%
!!! TACATATGTTTTGGAAGAAA+AGG - tig0031953:822-841 None:intergenic 25.0%
! TTCTTCCAAAACATATGTAT+AGG + tig0031953:823-842 Msa1448020:five_prime_UTR 25.0%
!! TATGCATAAAATTTGTTTCC+AGG + tig0031953:1379-1398 Msa1448020:intron 25.0%
!! ATGCATAAAATTTGTTTCCA+GGG + tig0031953:1380-1399 Msa1448020:intron 25.0%
! TTGTTGTGATATTTCTTAGT+TGG + tig0031953:1616-1635 Msa1448020:intron 25.0%
!! TATTTCTTAGTTGGTACATT+TGG + tig0031953:1625-1644 Msa1448020:intron 25.0%
!! ATTTCTTAGTTGGTACATTT+GGG + tig0031953:1626-1645 Msa1448020:intron 25.0%
! TGTTACTATAGTTGAATACA+AGG + tig0031953:1731-1750 Msa1448020:intron 25.0%
! TCTTAAAAAGACAACACAAA+TGG + tig0031953:1811-1830 Msa1448020:intron 25.0%
! CTAATATGTGAATAATCAAG+CGG + tig0031953:1836-1855 Msa1448020:intron 25.0%
!! TAATATGACTTGTGAAATGA+AGG + tig0031953:2038-2057 Msa1448020:intron 25.0%
!! AATATGACTTGTGAAATGAA+GGG + tig0031953:2039-2058 Msa1448020:intron 25.0%
! TTATAATACATACTCTGTGT+CGG - tig0031953:2490-2509 None:intergenic 25.0%
!! ACATTTTACTGAAGAAATTC+AGG - tig0031953:2690-2709 None:intergenic 25.0%
! CAAAGCATATATATACGTAT+TGG + tig0031953:2785-2804 Msa1448020:intron 25.0%
! AACTAATGAAGTTACTTCTT+GGG - tig0031953:2953-2972 None:intergenic 25.0%
! AAACTAATGAAGTTACTTCT+TGG - tig0031953:2954-2973 None:intergenic 25.0%
! AAGAAGTAACTTCATTAGTT+TGG + tig0031953:2953-2972 Msa1448020:intron 25.0%
! CTGAAATTAGAGTTCAAAAA+GGG + tig0031953:3076-3095 Msa1448020:intron 25.0%
!! CTTCTAAAGATTGTGTATAA+TGG + tig0031953:3374-3393 Msa1448020:CDS 25.0%
!! TTTTGTACAAAATCAAACCT+TGG + tig0031953:3483-3502 Msa1448020:intron 25.0%
! AGTATTATCAATCATCCAAT+AGG - tig0031953:3644-3663 None:intergenic 25.0%
!!! TATGTTTTTGATGGAACTAT+TGG + tig0031953:3683-3702 Msa1448020:intron 25.0%
! TTTCAATTTGAATTGTACTC+TGG + tig0031953:3709-3728 Msa1448020:intron 25.0%
!! AAGTAATTTGTCACAAACAT+CGG + tig0031953:3949-3968 Msa1448020:intron 25.0%
!! CTACAAGAAAAACATTGATT+TGG - tig0031953:4205-4224 None:intergenic 25.0%
!!! TTGGTTTGATCTTATTTTTC+AGG + tig0031953:4842-4861 Msa1448020:intron 25.0%
!! AAATTTTCCGACTTAGTTTA+TGG + tig0031953:5101-5120 Msa1448020:intron 25.0%
! TTTATAGTGAAAGCAAAACT+TGG + tig0031953:5141-5160 Msa1448020:intron 25.0%
! TTATAGTGAAAGCAAAACTT+GGG + tig0031953:5142-5161 Msa1448020:intron 25.0%
! TCATTAAGTTCAATTGTGTT+TGG - tig0031953:5196-5215 None:intergenic 25.0%
TGAGATTGAGGAATAAAATG+GGG - tig0031953:615-634 None:intergenic 30.0%
GTGAGATTGAGGAATAAAAT+GGG - tig0031953:616-635 None:intergenic 30.0%
AGTGAGATTGAGGAATAAAA+TGG - tig0031953:617-636 None:intergenic 30.0%
! GAGATTTAGGTTTCAATTTG+AGG - tig0031953:643-662 None:intergenic 30.0%
ATAATCCTTCGATTGAACAA+GGG - tig0031953:671-690 None:intergenic 30.0%
GCTTAAATGAAACTGGATAA+AGG - tig0031953:697-716 None:intergenic 30.0%
AGCAACTGTAGAAAAGAAAT+GGG - tig0031953:794-813 None:intergenic 30.0%
! CTTGTCCTATACATATGTTT+TGG - tig0031953:831-850 None:intergenic 30.0%
TTTCTGCTTAAGATTGAAGA+TGG + tig0031953:1073-1092 Msa1448020:five_prime_UTR 30.0%
TTAAGATTGAAGATGGACAA+GGG + tig0031953:1080-1099 Msa1448020:five_prime_UTR 30.0%
AAGCATGTGTTTATTGGATT+AGG + tig0031953:1103-1122 Msa1448020:five_prime_UTR 30.0%
AGCATGTGTTTATTGGATTA+GGG + tig0031953:1104-1123 Msa1448020:five_prime_UTR 30.0%
! GTTTATTGGATTAGGGTTAA+CGG + tig0031953:1111-1130 Msa1448020:five_prime_UTR 30.0%
! TCTCATGTTTGTGAACTTTT+AGG + tig0031953:1473-1492 Msa1448020:intron 30.0%
AGTAACACATGTAAAGAGAA+GGG - tig0031953:1718-1737 None:intergenic 30.0%
TAGTAACACATGTAAAGAGA+AGG - tig0031953:1719-1738 None:intergenic 30.0%
GTTGCAAGCTATAAACTATA+AGG + tig0031953:2223-2242 Msa1448020:intron 30.0%
CAGAAAATTGAATGAACAGA+CGG - tig0031953:2312-2331 None:intergenic 30.0%
CAATTTCTTCTGTCAATATC+TGG + tig0031953:2582-2601 Msa1448020:intron 30.0%
! AAGTAACTTCATTAGTTTGG+TGG + tig0031953:2956-2975 Msa1448020:intron 30.0%
GCTGAAATTAGAGTTCAAAA+AGG + tig0031953:3075-3094 Msa1448020:intron 30.0%
TAAAGTCATTACCCTTAAGT+TGG - tig0031953:3217-3236 None:intergenic 30.0%
TCTGCTTTGATAGAGAAAAA+AGG + tig0031953:3305-3324 Msa1448020:CDS 30.0%
TCAAACCTTGGATATTTCTT+AGG + tig0031953:3495-3514 Msa1448020:intron 30.0%
ATTTGCCTAAGAAATATCCA+AGG - tig0031953:3503-3522 None:intergenic 30.0%
! TTTGTAGACCTTCAAGTTAT+AGG + tig0031953:3614-3633 Msa1448020:intron 30.0%
TAGGATAACCTATAACTTGA+AGG - tig0031953:3625-3644 None:intergenic 30.0%
CAAGTTATAGGTTATCCTAT+TGG + tig0031953:3626-3645 Msa1448020:intron 30.0%
! AGTAATTTGTCACAAACATC+GGG + tig0031953:3950-3969 Msa1448020:intron 30.0%
!! AGAAAAACATTGATTTGGGA+CGG - tig0031953:4200-4219 None:intergenic 30.0%
!! ATGGAGCATTTTAAGAGAAT+CGG - tig0031953:4343-4362 None:intergenic 30.0%
!! TTTTCTAGAGAAGCTACTTA+AGG + tig0031953:4620-4639 Msa1448020:intron 30.0%
TTTCCCTGATAATCACATAT+TGG + tig0031953:4881-4900 Msa1448020:CDS 30.0%
CTTCCAATATGTGATTATCA+GGG - tig0031953:4887-4906 None:intergenic 30.0%
AAACTCTTGATTGTATCTGA+TGG - tig0031953:4921-4940 None:intergenic 30.0%
ATAAAATGGCTTATCTGGTT+GGG - tig0031953:4970-4989 None:intergenic 30.0%
AATAAAATGGCTTATCTGGT+TGG - tig0031953:4971-4990 None:intergenic 30.0%
CACAAATAAAATGGCTTATC+TGG - tig0031953:4975-4994 None:intergenic 30.0%
! GTTGTTTCCATAAACTAAGT+CGG - tig0031953:5111-5130 None:intergenic 30.0%
GTTTATGGAAACAACTTATG+CGG + tig0031953:5116-5135 Msa1448020:intron 30.0%
AAATCTTTGATCAGCTTCTT+AGG - tig0031953:5259-5278 None:intergenic 30.0%
TCCAGCAGTATAAAAATCTT+GGG - tig0031953:5284-5303 None:intergenic 30.0%
ATCCAGCAGTATAAAAATCT+TGG - tig0031953:5285-5304 None:intergenic 30.0%
TCAATATGATAGGTACACAA+TGG - tig0031953:5320-5339 None:intergenic 30.0%
!!! TTTCATGGTTTTGGAGTTGT+AGG - tig0031953:592-611 None:intergenic 35.0%
! AAAATGGGGTTTCATGGTTT+TGG - tig0031953:601-620 None:intergenic 35.0%
AGGAATAAAATGGGGTTTCA+TGG - tig0031953:607-626 None:intergenic 35.0%
CATAATCCTTCGATTGAACA+AGG - tig0031953:672-691 None:intergenic 35.0%
!! ACGGAATGGGTTAAAACTAT+GGG - tig0031953:725-744 None:intergenic 35.0%
!! AACGGAATGGGTTAAAACTA+TGG - tig0031953:726-745 None:intergenic 35.0%
AGAAAAGAAATGGGTCGAAA+AGG - tig0031953:785-804 None:intergenic 35.0%
GAGCAACTGTAGAAAAGAAA+TGG - tig0031953:795-814 None:intergenic 35.0%
CTTAAGATTGAAGATGGACA+AGG + tig0031953:1079-1098 Msa1448020:five_prime_UTR 35.0%
TGTGAGAAGAGAACATGATA+AGG + tig0031953:1186-1205 Msa1448020:five_prime_UTR 35.0%
TTAAGCCTATGATTAACAGC+AGG - tig0031953:1234-1253 None:intergenic 35.0%
TGCTGTTAATCATAGGCTTA+AGG + tig0031953:1233-1252 Msa1448020:five_prime_UTR 35.0%
! TAGACATTGGTTAGACACAT+GGG - tig0031953:1448-1467 None:intergenic 35.0%
! ATAGACATTGGTTAGACACA+TGG - tig0031953:1449-1468 None:intergenic 35.0%
ACATGAGACAGTATAGACAT+TGG - tig0031953:1461-1480 None:intergenic 35.0%
TATAGTTGAATACAAGGTCG+TGG + tig0031953:1737-1756 Msa1448020:intron 35.0%
AGACATAAGGGCTAAAAGAA+AGG + tig0031953:2084-2103 Msa1448020:intron 35.0%
TGATATGCATGTTAAGAACG+AGG - tig0031953:2134-2153 None:intergenic 35.0%
CAACACATATATCTTTCCTC+AGG - tig0031953:2196-2215 None:intergenic 35.0%
!! AATACCTATGACTTGCGTTT+TGG - tig0031953:2379-2398 None:intergenic 35.0%
TCATATGCACATTCAGAAAG+AGG - tig0031953:2419-2438 None:intergenic 35.0%
ATTGACACACATTCGTAAAG+AGG - tig0031953:2566-2585 None:intergenic 35.0%
TTCTTCTGTCAATATCTGGA+TGG + tig0031953:2586-2605 Msa1448020:intron 35.0%
! GTCTTTTCTGATATGTTCTG+CGG + tig0031953:2608-2627 Msa1448020:intron 35.0%
!!! TTTTAGAACCTACGCTTCAA+TGG - tig0031953:2660-2679 None:intergenic 35.0%
AGCTCTCTTGAAATAGTGTT+AGG + tig0031953:2925-2944 Msa1448020:intron 35.0%
AAAAGGGCTATGATATCATC+AGG + tig0031953:3092-3111 Msa1448020:intron 35.0%
AAAGGGCTATGATATCATCA+GGG + tig0031953:3093-3112 Msa1448020:intron 35.0%
AGAAGCTAATGACCAACTTA+AGG + tig0031953:3202-3221 Msa1448020:five_prime_UTR 35.0%
GAAGCTAATGACCAACTTAA+GGG + tig0031953:3203-3222 Msa1448020:five_prime_UTR 35.0%
CTTAAACATGTGTACACCAA+AGG - tig0031953:3442-3461 None:intergenic 35.0%
!!! TATTTTAACCCAATCTGGGA+CGG + tig0031953:3921-3940 Msa1448020:intron 35.0%
!! TACTTGCTGTCGCAATATTT+CGG - tig0031953:4095-4114 None:intergenic 35.0%
AAATTTGTACTACTGTGCGA+CGG + tig0031953:4168-4187 Msa1448020:intron 35.0%
GGGGACCAATATTTAAAAGT+TGG - tig0031953:4236-4255 None:intergenic 35.0%
! TTTTAAATATTGGTCCCCCA+TGG + tig0031953:4238-4257 Msa1448020:intron 35.0%
GCTTCCAATATGTGATTATC+AGG - tig0031953:4888-4907 None:intergenic 35.0%
! GATTGTATCTGATGGATGAA+AGG - tig0031953:4913-4932 None:intergenic 35.0%
! TCCCAAGATTTTTATACTGC+TGG + tig0031953:5280-5299 Msa1448020:CDS 35.0%
TGTAGGAACGAGACTGAAAT+TGG - tig0031953:575-594 None:intergenic 40.0%
TTTGAGGGAAAGTGAGATTG+AGG - tig0031953:627-646 None:intergenic 40.0%
AACAAGGGTGGTAGAGATTT+AGG - tig0031953:656-675 None:intergenic 40.0%
GGTGAGAGCTTAAATGAAAC+TGG - tig0031953:704-723 None:intergenic 40.0%
AGTGAAGAACTGTGGAGAAA+CGG - tig0031953:744-763 None:intergenic 40.0%
CAACAAAGAGTGAAGAACTG+TGG - tig0031953:752-771 None:intergenic 40.0%
! CAAGGGAAGCATGTGTTTAT+TGG + tig0031953:1097-1116 Msa1448020:five_prime_UTR 40.0%
AGAGAACATGATAAGGAGTC+TGG + tig0031953:1193-1212 Msa1448020:five_prime_UTR 40.0%
!! TTTGTTTCCAGGGTACTGTT+AGG + tig0031953:1390-1409 Msa1448020:intron 40.0%
GTAAGACAGTTGAAGAAGTG+TGG - tig0031953:1677-1696 None:intergenic 40.0%
CTGTCTTACATCACATCTCA+AGG + tig0031953:1688-1707 Msa1448020:five_prime_UTR 40.0%
TTTCAACCAAATGAGCTCAC+AGG - tig0031953:1772-1791 None:intergenic 40.0%
CTCATGAAAAAACAGACCAG+AGG - tig0031953:1874-1893 None:intergenic 40.0%
ATTCCTTCTAATGCAACCTG+AGG + tig0031953:2177-2196 Msa1448020:intron 40.0%
! TTTCCTCAGGTTGCATTAGA+AGG - tig0031953:2183-2202 None:intergenic 40.0%
CTCTGATTATCTTTAGCGAC+AGG + tig0031953:2455-2474 Msa1448020:intron 40.0%
TTGCAAAACCATTGAAGCGT+AGG + tig0031953:2649-2668 Msa1448020:intron 40.0%
TGCTATGCTCATTCAGATAG+AGG - tig0031953:2715-2734 None:intergenic 40.0%
TGATATCATCAGGGTGAGTT+TGG + tig0031953:3102-3121 Msa1448020:intron 40.0%
! CTAGTTGTTCTTTTGTCGAC+AGG + tig0031953:3136-3155 Msa1448020:intron 40.0%
ACAGGAGTTGAAGTAGAGAA+AGG + tig0031953:3154-3173 Msa1448020:intron 40.0%
GATGATGCTTTCACCGTTAA+TGG - tig0031953:3287-3306 None:intergenic 40.0%
! AAAGGCATGTGTTACGTCAA+TGG + tig0031953:3323-3342 Msa1448020:CDS 40.0%
TGATAGCTTGACATTGCCTT+TGG + tig0031953:3423-3442 Msa1448020:intron 40.0%
TTAGGCAAATCAACAACTGC+TGG + tig0031953:3513-3532 Msa1448020:intron 40.0%
ATTTGTCACAAACATCGGGA+CGG + tig0031953:3954-3973 Msa1448020:intron 40.0%
TGAGATTTCTGTCGCAATGT+TGG + tig0031953:3981-4000 Msa1448020:intron 40.0%
GAGATTTCTGTCGCAATGTT+GGG + tig0031953:3982-4001 Msa1448020:intron 40.0%
TTGGGACGGAATTGAATAGT+TGG + tig0031953:4000-4019 Msa1448020:intron 40.0%
TGGGACGGAATTGAATAGTT+GGG + tig0031953:4001-4020 Msa1448020:intron 40.0%
!! ACTTGCTGTCGCAATATTTC+GGG - tig0031953:4094-4113 None:intergenic 40.0%
!! TGAAATCTGGGACGGAATTT+TGG - tig0031953:4138-4157 None:intergenic 40.0%
ATTCCGTCCCAGATTTCAAT+TGG + tig0031953:4140-4159 Msa1448020:intron 40.0%
TTCCGTCCCAGATTTCAATT+GGG + tig0031953:4141-4160 Msa1448020:intron 40.0%
TTCCGTCCCAATTGAAATCT+GGG - tig0031953:4150-4169 None:intergenic 40.0%
TTTCCGTCCCAATTGAAATC+TGG - tig0031953:4151-4170 None:intergenic 40.0%
TCGCAGGTCAAGAATAATCA+AGG + tig0031953:4443-4462 Msa1448020:CDS 40.0%
TCTTTGATCAGCTTCTTAGG+TGG - tig0031953:5256-5275 None:intergenic 40.0%
! TATACTGCTGGATTTTGTCC+TGG + tig0031953:5292-5311 Msa1448020:CDS 40.0%
TACCACCCTTGTTCAATCGA+AGG + tig0031953:663-682 Msa1448020:five_prime_UTR 45.0%
ATCCTTCGATTGAACAAGGG+TGG - tig0031953:668-687 None:intergenic 45.0%
GAACTGTGGAGAAACGGAAT+GGG - tig0031953:738-757 None:intergenic 45.0%
AGAACTGTGGAGAAACGGAA+TGG - tig0031953:739-758 None:intergenic 45.0%
GGTTCCAATCAGACAAGATC+TGG + tig0031953:903-922 Msa1448020:five_prime_UTR 45.0%
CAATCAGACAAGATCTGGTC+CGG + tig0031953:908-927 Msa1448020:five_prime_UTR 45.0%
CTTCTAACCGTATCTCACAC+CGG - tig0031953:961-980 None:intergenic 45.0%
CGGTTAGAAGATCCCAACTT+CGG + tig0031953:971-990 Msa1448020:five_prime_UTR 45.0%
! TGTTTTTCCCTGTCAGTGTG+AGG + tig0031953:1015-1034 Msa1448020:five_prime_UTR 45.0%
! GTTTTTCCCTGTCAGTGTGA+GGG + tig0031953:1016-1035 Msa1448020:five_prime_UTR 45.0%
! TTGGATTAGGGTTAACGGAG+AGG + tig0031953:1116-1135 Msa1448020:five_prime_UTR 45.0%
GATTAACAGCAGGATGGCAT+TGG - tig0031953:1224-1243 None:intergenic 45.0%
GCCATCCTGCTGTTAATCAT+AGG + tig0031953:1226-1245 Msa1448020:five_prime_UTR 45.0%
GCCTATGATTAACAGCAGGA+TGG - tig0031953:1230-1249 None:intergenic 45.0%
AAGTTTGCCTAACAGTACCC+TGG - tig0031953:1400-1419 None:intergenic 45.0%
TGAATACAAGGTCGTGGCAT+TGG + tig0031953:1743-1762 Msa1448020:intron 45.0%
AGATTTAGCCTGTCGAGTGA+TGG + tig0031953:2343-2362 Msa1448020:intron 45.0%
GTAATGTTCCATCACTCGAC+AGG - tig0031953:2354-2373 None:intergenic 45.0%
ACTGCCAAAACGCAAGTCAT+AGG + tig0031953:2372-2391 Msa1448020:intron 45.0%
! CATACTCTGTGTCGGTACAA+AGG - tig0031953:2482-2501 None:intergenic 45.0%
TTTCTGTCGCAATGTTGGGA+CGG + tig0031953:3986-4005 Msa1448020:intron 45.0%
GGGACGGAATTGAATAGTTG+GGG + tig0031953:4002-4021 Msa1448020:intron 45.0%
GTCCCAATTGAAATCTGGGA+CGG - tig0031953:4146-4165 None:intergenic 45.0%
GTCCCAGATTTCAATTGGGA+CGG + tig0031953:4145-4164 Msa1448020:intron 45.0%
TAAATATTGGTCCCCCATGG+TGG + tig0031953:4241-4260 Msa1448020:intron 45.0%
TCGGCTTCTCTGAGTTTCTT+AGG - tig0031953:4414-4433 None:intergenic 45.0%
!!! ACTTTAAAATAATATTTTAT+TGG + tig0031953:1922-1941 Msa1448020:intron 5.0%
!!! TTTTTAACAATATTATATTT+TGG - tig0031953:2834-2853 None:intergenic 5.0%
TTAGCCGCATTTGTAACCGC+CGG - tig0031953:882-901 None:intergenic 50.0%
GCGGTTACAAATGCGGCTAA+TGG + tig0031953:882-901 Msa1448020:five_prime_UTR 50.0%
CGGACCAGATCTTGTCTGAT+TGG - tig0031953:910-929 None:intergenic 50.0%
ACTGAACCCTCACACTGACA+GGG - tig0031953:1025-1044 None:intergenic 50.0%
AACTGAACCCTCACACTGAC+AGG - tig0031953:1026-1045 None:intergenic 50.0%
GTGTGAGGGTTCAGTTGAAC+TGG + tig0031953:1030-1049 Msa1448020:five_prime_UTR 50.0%
TGGACAACGACAACACTCGA+CGG - tig0031953:1657-1676 None:intergenic 50.0%
TGGTGACCTGTGAGCTCATT+TGG + tig0031953:1763-1782 Msa1448020:intron 50.0%
GCGGCATTGCATATTACCTC+TGG + tig0031953:1855-1874 Msa1448020:intron 50.0%
GACGGACATGCATGTGCAAA+AGG - tig0031953:2294-2313 None:intergenic 50.0%
TCAACAACTGCTGGTGTACG+TGG + tig0031953:3522-3541 Msa1448020:intron 50.0%
TTCAGCACTGCTAAGACTCG+AGG + tig0031953:3554-3573 Msa1448020:intron 50.0%
CTTGCATTCCGTCCCAGATT+GGG - tig0031953:3932-3951 None:intergenic 50.0%
ACTTGCATTCCGTCCCAGAT+TGG - tig0031953:3933-3952 None:intergenic 50.0%
TTCATCTTCCGTCGCACGTT+GGG - tig0031953:4064-4083 None:intergenic 50.0%
TTTCATCTTCCGTCGCACGT+TGG - tig0031953:4065-4084 None:intergenic 50.0%
GCTGTCGCAATATTTCGGGA+CGG - tig0031953:4090-4109 None:intergenic 50.0%
TCCGATCCAAAGACCACCAT+GGG - tig0031953:4257-4276 None:intergenic 50.0%
ATCCGATCCAAAGACCACCA+TGG - tig0031953:4258-4277 None:intergenic 50.0%
GAAACTCAGAGAAGCCGAAG+TGG + tig0031953:4416-4435 Msa1448020:CDS 50.0%
GATAGGTACACAATGGCACC+AGG - tig0031953:5313-5332 None:intergenic 50.0%
ACCTCCGGCGGTTACAAATG+CGG + tig0031953:875-894 Msa1448020:five_prime_UTR 55.0%
ACCGTATCTCACACCGGTCA+CGG - tig0031953:955-974 None:intergenic 55.0%
GCGAAGAGATCGCCGAAGTT+GGG - tig0031953:986-1005 None:intergenic 55.0%
AGCGAAGAGATCGCCGAAGT+TGG - tig0031953:987-1006 None:intergenic 55.0%
TCATCTTCCGTCGCACGTTG+GGG - tig0031953:4063-4082 None:intergenic 55.0%
CGATCCAAAGACCACCATGG+GGG - tig0031953:4255-4274 None:intergenic 55.0%
CCGATCCAAAGACCACCATG+GGG - tig0031953:4256-4275 None:intergenic 55.0%
CCCCATGGTGGTCTTTGGAT+CGG + tig0031953:4253-4272 Msa1448020:intron 55.0%
! TGACCTGCGAGATGCCACTT+CGG - tig0031953:4433-4452 None:intergenic 55.0%
ATCCCGCCACGAACAACCTC+CGG + tig0031953:860-879 Msa1448020:five_prime_UTR 60.0%
TAACCGCCGGAGGTTGTTCG+TGG - tig0031953:869-888 None:intergenic 60.0%
TATCTCACACCGGTCACGGC+GGG - tig0031953:951-970 None:intergenic 60.0%
GTATCTCACACCGGTCACGG+CGG - tig0031953:952-971 None:intergenic 60.0%
GCCGTGACCGGTGTGAGATA+CGG + tig0031953:951-970 Msa1448020:five_prime_UTR 60.0%
GTTGGGGACGGAATCTGGGA+CGG - tig0031953:4047-4066 None:intergenic 60.0%
GCACGTTGGGGACGGAATCT+GGG - tig0031953:4051-4070 None:intergenic 60.0%
ATTCCGTCCCCAACGTGCGA+CGG + tig0031953:4053-4072 Msa1448020:intron 60.0%
TGGTCCCCCATGGTGGTCTT+TGG + tig0031953:4248-4267 Msa1448020:intron 60.0%
AAGCCGAAGTGGCATCTCGC+AGG + tig0031953:4427-4446 Msa1448020:CDS 60.0%
GCCGCATTTGTAACCGCCGG+AGG - tig0031953:879-898 None:intergenic 65.0%
CGCACGTTGGGGACGGAATC+TGG - tig0031953:4052-4071 None:intergenic 65.0%
CTTCCGTCGCACGTTGGGGA+CGG - tig0031953:4059-4078 None:intergenic 65.0%
CGCCGGAGGTTGTTCGTGGC+GGG - tig0031953:865-884 None:intergenic 70.0%
CCGCCGGAGGTTGTTCGTGG+CGG - tig0031953:866-885 None:intergenic 70.0%
CCGCCACGAACAACCTCCGG+CGG + tig0031953:863-882 Msa1448020:five_prime_UTR 70.0%
GGACACAACGCGGAGACGGC+CGG - tig0031953:930-949 None:intergenic 70.0%
GGCGGGACACAACGCGGAGA+CGG - tig0031953:934-953 None:intergenic 70.0%
GGTCACGGCGGGACACAACG+CGG - tig0031953:940-959 None:intergenic 70.0%
GCGTTGTGTCCCGCCGTGAC+CGG + tig0031953:939-958 Msa1448020:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
tig0031953 gene 534 5339 534 ID=Msa1448020;Name=Msa1448020
tig0031953 mRNA 534 5339 534 ID=Msa1448020-mRNA-1;Parent=Msa1448020;Name=Msa1448020-mRNA-1;_AED=0.28;_eAED=0.28;_QI=919|0.4|0.5|1|0.8|0.83|6|0|135
tig0031953 exon 534 1254 534 ID=Msa1448020-mRNA-1:exon:16198;Parent=Msa1448020-mRNA-1
tig0031953 exon 1635 1709 1635 ID=Msa1448020-mRNA-1:exon:16199;Parent=Msa1448020-mRNA-1
tig0031953 exon 3158 3395 3158 ID=Msa1448020-mRNA-1:exon:16200;Parent=Msa1448020-mRNA-1
tig0031953 exon 4397 4464 4397 ID=Msa1448020-mRNA-1:exon:16201;Parent=Msa1448020-mRNA-1
tig0031953 exon 4864 4993 4864 ID=Msa1448020-mRNA-1:exon:16202;Parent=Msa1448020-mRNA-1
tig0031953 exon 5245 5339 5245 ID=Msa1448020-mRNA-1:exon:16203;Parent=Msa1448020-mRNA-1
tig0031953 five_prime_UTR 534 1254 534 ID=Msa1448020-mRNA-1:five_prime_utr;Parent=Msa1448020-mRNA-1
tig0031953 five_prime_UTR 1635 1709 1635 ID=Msa1448020-mRNA-1:five_prime_utr;Parent=Msa1448020-mRNA-1
tig0031953 five_prime_UTR 3158 3280 3158 ID=Msa1448020-mRNA-1:five_prime_utr;Parent=Msa1448020-mRNA-1
tig0031953 CDS 3281 3395 3281 ID=Msa1448020-mRNA-1:cds;Parent=Msa1448020-mRNA-1
tig0031953 CDS 4397 4464 4397 ID=Msa1448020-mRNA-1:cds;Parent=Msa1448020-mRNA-1
tig0031953 CDS 4864 4993 4864 ID=Msa1448020-mRNA-1:cds;Parent=Msa1448020-mRNA-1
tig0031953 CDS 5245 5339 5245 ID=Msa1448020-mRNA-1:cds;Parent=Msa1448020-mRNA-1
Gene Sequence

>Msa1448020

ATGCCATTAACGGTGAAAGCATCATCTGCTTTGATAGAGAAAAAAGGCATGTGTTACGTCAATGGATCTCGAATAATGAAGAGTATTTGTCATCTTCTAAAGATTGTGTATAATGATAAATTCTTGAAACCTAAGAAACTCAGAGAAGCCGAAGTGGCATCTCGCAGGTCAAGAATAATCAAGGCTGTAATTAGAGTTCGTTTCCCTGATAATCACATATTGGAAGCTACCTTTCATCCATCAGATACAATCAAGAGTTTAATTGATCTCATCAATAAAGAGATTGCCCAACCAGATAAGCCATTTTATTTGTATACTACTCCACCTAAGAAGCTGATCAAAGATTTCTCCCAAGATTTTTATACTGCTGGATTTTGTCCTGGTGCCATTGTGTACCTATCATATTGA
Protein sequence

>Msa1448020

MPLTVKASSALIEKKGMCYVNGSRIMKSICHLLKIVYNDKFLKPKKLREAEVASRRSRIIKAVIRVRFPDNHILEATFHPSDTIKSLIDLINKEIAQPDKPFYLYTTPPKKLIKDFSQDFYTAGFCPGAIVYLSY*