AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0456420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0456420 MtrunA17_Chr3g0103371 94.000 150 7 2 1 149 1 149 6.25e-88 253
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0456420 AT2G01870.1 42.453 106 45 5 51 149 30 126 1.45e-15 69.7

Find 44 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTCTCGTGTCTTAAAATT+TGG 0.112904 3_3:-49994592 Msa0456420:five_prime_UTR
GGAAATGAAGATAATGTTTA+TGG 0.128032 3_3:+49994618 None:intergenic
TCAACTTAGGAAAGAATATT+TGG 0.173434 3_3:-49992465 Msa0456420:three_prime_UTR
AAGCTCTTTGAGGGCTTCTT+TGG 0.197880 3_3:+49994321 None:intergenic
AGGGCTTCTTTGGCTTTCTC+TGG 0.231668 3_3:+49994331 None:intergenic
CGGAGAGTACCCTTGAGTTT+TGG 0.261285 3_3:+49994256 None:intergenic
CCTCTCGTGTCTTAAAATTT+GGG 0.270427 3_3:-49994591 Msa0456420:five_prime_UTR
CTGCATAGTTCATGCATTAA+AGG 0.282045 3_3:-49994380 Msa0456420:CDS
GTGTGATATTAATTAATTTA+CGG 0.287568 3_3:-49992609 Msa0456420:three_prime_UTR
TTATTACATAATAATGATAA+AGG 0.364645 3_3:-49994454 Msa0456420:CDS
GAAAGTGATGCAAATAATAA+TGG 0.386523 3_3:+49994487 None:intergenic
TCCTTTCTTGCACTTCTAGC+TGG 0.396441 3_3:-49992726 Msa0456420:CDS
GTAATTAAGCCAGCCATTGA+TGG 0.399846 3_3:-49992657 Msa0456420:CDS
TCGCTGCATATATAAAGTTA+AGG 0.408502 3_3:-49992581 Msa0456420:three_prime_UTR
ACAATTGATGAAAATTTAAG+TGG 0.411884 3_3:+49992440 None:intergenic
CTGCACTTGGATATAGGTTA+TGG 0.412024 3_3:+49992633 None:intergenic
GCATATATAAAGTTAAGGTT+TGG 0.413561 3_3:-49992576 Msa0456420:three_prime_UTR
TTTCTGGTCAAGCTCTTTGA+GGG 0.426726 3_3:+49994312 None:intergenic
CACACACTGCACTTGGATAT+AGG 0.429892 3_3:+49992627 None:intergenic
GGGCTTCTTTGGCTTTCTCT+GGG 0.431656 3_3:+49994332 None:intergenic
CTCTCGTGTCTTAAAATTTG+GGG 0.444670 3_3:-49994590 Msa0456420:five_prime_UTR
TTAATATCACACACTGCACT+TGG 0.458769 3_3:+49992620 None:intergenic
TCATTATTATGTAATAATAA+CGG 0.468150 3_3:+49994460 None:intergenic
TTTCTTGCACTTCTAGCTGG+TGG 0.493384 3_3:-49992723 Msa0456420:CDS
ACCAGCTAGAAGTGCAAGAA+AGG 0.499982 3_3:+49992725 None:intergenic
GGTGGCTGCTTGATGCTAAG+AGG 0.527505 3_3:+49994508 None:intergenic
TATAGGTTATGGACCATCAA+TGG 0.534653 3_3:+49992644 None:intergenic
GACAACATTAAAATCAACTT+AGG 0.546871 3_3:-49992478 Msa0456420:three_prime_UTR
TGGCTGGCTTAATTACAGTA+AGG 0.551216 3_3:+49992664 None:intergenic
AGTTTCCTCTCCAAAACTCA+AGG 0.567427 3_3:-49994266 Msa0456420:CDS
GGTTATGGACCATCAATGGC+TGG 0.567707 3_3:+49992648 None:intergenic
GGGAGTGTAGTGTAGAGTGA+AGG 0.571725 3_3:-49994569 Msa0456420:five_prime_UTR
CTTATCACCTCCTCTCTCGT+AGG 0.590439 3_3:+49992780 None:intergenic
TCTCGTGTCTTAAAATTTGG+GGG 0.607891 3_3:-49994589 Msa0456420:five_prime_UTR
ATAAAGGCATATTCAAAGAG+AGG 0.608157 3_3:-49994438 Msa0456420:CDS
GTTTCCTCTCCAAAACTCAA+GGG 0.619477 3_3:-49994265 Msa0456420:intron
GAATAACATCTGAATATGAG+TGG 0.621057 3_3:+49994540 None:intergenic
AGAGATGAAGCCTACGAGAG+AGG 0.636132 3_3:-49992790 Msa0456420:CDS
CAGAATCTTAGCAACATGCG+TGG 0.639323 3_3:+49992503 None:intergenic
AGAATCTTAGCAACATGCGT+GGG 0.645379 3_3:+49992504 None:intergenic
AAGGCATATTCAAAGAGAGG+AGG 0.658459 3_3:-49994435 Msa0456420:CDS
GATGAAGCCTACGAGAGAGG+AGG 0.660242 3_3:-49992787 Msa0456420:CDS
AGTGATGCAAATAATAATGG+TGG 0.676828 3_3:+49994490 None:intergenic
GAGGTGATAAGTGACAACAA+TGG 0.780630 3_3:-49992768 Msa0456420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATATTTTAAATTTTTA+TGG + chr3_3:49993649-49993668 None:intergenic 0.0%
!!! TATAATATTTTAAATTTTTA+TGG + chr3_3:49993649-49993668 None:intergenic 0.0%
!! TCATTATTATGTAATAATAA+CGG + chr3_3:49992586-49992605 None:intergenic 10.0%
!! TTATTACATAATAATGATAA+AGG - chr3_3:49992589-49992608 Msa0456420:three_prime_UTR 10.0%
!!! AAAAAATTCTGAATTTTTTT+TGG + chr3_3:49993681-49993700 None:intergenic 10.0%
!! TCATTATTATGTAATAATAA+CGG + chr3_3:49992586-49992605 None:intergenic 10.0%
!! TTATTACATAATAATGATAA+AGG - chr3_3:49992589-49992608 Msa0456420:three_prime_UTR 10.0%
!!! AAAAAATTCTGAATTTTTTT+TGG + chr3_3:49993681-49993700 None:intergenic 10.0%
!!! ATTTCCCTTTTTTAATTTTA+CGG - chr3_3:49992953-49992972 Msa0456420:intron 15.0%
!! AAAACCGTAAAATTAAAAAA+GGG + chr3_3:49992960-49992979 None:intergenic 15.0%
!!! TTTGGAAAAAACAATTATTT+TGG - chr3_3:49992991-49993010 Msa0456420:intron 15.0%
!! TGAAATGTAAATTTAGAAAA+GGG + chr3_3:49993174-49993193 None:intergenic 15.0%
!! ATGAAATGTAAATTTAGAAA+AGG + chr3_3:49993175-49993194 None:intergenic 15.0%
!! ATTATTCAACATATTTGAAT+TGG + chr3_3:49993262-49993281 None:intergenic 15.0%
!!! AAACTAAAAGTATTTATAAG+TGG + chr3_3:49993436-49993455 None:intergenic 15.0%
!!! TGAAGATTGAAAATTTTATA+GGG + chr3_3:49993565-49993584 None:intergenic 15.0%
!!! TATGAATTTACTTTAAAAGA+GGG + chr3_3:49993596-49993615 None:intergenic 15.0%
!!! ATATGAATTTACTTTAAAAG+AGG + chr3_3:49993597-49993616 None:intergenic 15.0%
!!! AAATTTTCAATCACTATTTT+TGG + chr3_3:49993875-49993894 None:intergenic 15.0%
!!! ATAGTGATTGAAAATTTTAT+AGG - chr3_3:49993878-49993897 Msa0456420:intron 15.0%
!!! TAGTGATTGAAAATTTTATA+GGG - chr3_3:49993879-49993898 Msa0456420:intron 15.0%
!! GTGTGATATTAATTAATTTA+CGG - chr3_3:49994434-49994453 Msa0456420:CDS 15.0%
!!! ATTTCCCTTTTTTAATTTTA+CGG - chr3_3:49992953-49992972 Msa0456420:intron 15.0%
!! AAAACCGTAAAATTAAAAAA+GGG + chr3_3:49992960-49992979 None:intergenic 15.0%
!!! TTTGGAAAAAACAATTATTT+TGG - chr3_3:49992991-49993010 Msa0456420:intron 15.0%
!! TGAAATGTAAATTTAGAAAA+GGG + chr3_3:49993174-49993193 None:intergenic 15.0%
!! ATGAAATGTAAATTTAGAAA+AGG + chr3_3:49993175-49993194 None:intergenic 15.0%
!! ATTATTCAACATATTTGAAT+TGG + chr3_3:49993262-49993281 None:intergenic 15.0%
!!! AAACTAAAAGTATTTATAAG+TGG + chr3_3:49993436-49993455 None:intergenic 15.0%
!!! TGAAGATTGAAAATTTTATA+GGG + chr3_3:49993565-49993584 None:intergenic 15.0%
!!! TATGAATTTACTTTAAAAGA+GGG + chr3_3:49993596-49993615 None:intergenic 15.0%
!!! ATATGAATTTACTTTAAAAG+AGG + chr3_3:49993597-49993616 None:intergenic 15.0%
!!! AAATTTTCAATCACTATTTT+TGG + chr3_3:49993875-49993894 None:intergenic 15.0%
!!! ATAGTGATTGAAAATTTTAT+AGG - chr3_3:49993878-49993897 Msa0456420:intron 15.0%
!!! TAGTGATTGAAAATTTTATA+GGG - chr3_3:49993879-49993898 Msa0456420:intron 15.0%
!! GTGTGATATTAATTAATTTA+CGG - chr3_3:49994434-49994453 Msa0456420:CDS 15.0%
!! ATAAGAGAAAAAGAAAGAAA+CGG + chr3_3:49992810-49992829 None:intergenic 20.0%
!!! TGTAGTGTTTTTAAATTTTG+TGG - chr3_3:49992851-49992870 Msa0456420:intron 20.0%
!! CAAAACCGTAAAATTAAAAA+AGG + chr3_3:49992961-49992980 None:intergenic 20.0%
!!! TTGCATTTTATTTTGAGTTA+GGG + chr3_3:49993047-49993066 None:intergenic 20.0%
!!! TTTGCATTTTATTTTGAGTT+AGG + chr3_3:49993048-49993067 None:intergenic 20.0%
!! AATTGACAACTGTAATAATA+AGG - chr3_3:49993118-49993137 Msa0456420:intron 20.0%
!! ACATCATATATAGTTAATCT+TGG + chr3_3:49993230-49993249 None:intergenic 20.0%
!!! TTTTGGGTTAAATATGTTTT+TGG - chr3_3:49993478-49993497 Msa0456420:intron 20.0%
!! GAAAAGTTGACATATTTATA+GGG + chr3_3:49993939-49993958 None:intergenic 20.0%
!!! GTGAAGATTGAAAATTTTAT+AGG + chr3_3:49993566-49993585 None:intergenic 20.0%
!!! AAATTTTCAATCTTCACTTT+TGG - chr3_3:49993569-49993588 Msa0456420:intron 20.0%
!! GAAAAGTTGACATATTTATA+GGG - chr3_3:49993939-49993958 Msa0456420:intron 20.0%
!! GTTGGAAATTAAAAAGTTTA+GGG + chr3_3:49993982-49994001 None:intergenic 20.0%
!! ACAATTGATGAAAATTTAAG+TGG + chr3_3:49994606-49994625 None:intergenic 20.0%
!! ATAAGAGAAAAAGAAAGAAA+CGG + chr3_3:49992810-49992829 None:intergenic 20.0%
!!! TGTAGTGTTTTTAAATTTTG+TGG - chr3_3:49992851-49992870 Msa0456420:intron 20.0%
!! CAAAACCGTAAAATTAAAAA+AGG + chr3_3:49992961-49992980 None:intergenic 20.0%
!!! TTGCATTTTATTTTGAGTTA+GGG + chr3_3:49993047-49993066 None:intergenic 20.0%
!!! TTTGCATTTTATTTTGAGTT+AGG + chr3_3:49993048-49993067 None:intergenic 20.0%
!! AATTGACAACTGTAATAATA+AGG - chr3_3:49993118-49993137 Msa0456420:intron 20.0%
!! ACATCATATATAGTTAATCT+TGG + chr3_3:49993230-49993249 None:intergenic 20.0%
!!! TTTTGGGTTAAATATGTTTT+TGG - chr3_3:49993478-49993497 Msa0456420:intron 20.0%
!! GAAAAGTTGACATATTTATA+GGG + chr3_3:49993939-49993958 None:intergenic 20.0%
!!! GTGAAGATTGAAAATTTTAT+AGG + chr3_3:49993566-49993585 None:intergenic 20.0%
!!! AAATTTTCAATCTTCACTTT+TGG - chr3_3:49993569-49993588 Msa0456420:intron 20.0%
!! GAAAAGTTGACATATTTATA+GGG - chr3_3:49993939-49993958 Msa0456420:intron 20.0%
!! GTTGGAAATTAAAAAGTTTA+GGG + chr3_3:49993982-49994001 None:intergenic 20.0%
!! ACAATTGATGAAAATTTAAG+TGG + chr3_3:49994606-49994625 None:intergenic 20.0%
! GGAAATGAAGATAATGTTTA+TGG + chr3_3:49992428-49992447 None:intergenic 25.0%
! GAAAGTGATGCAAATAATAA+TGG + chr3_3:49992559-49992578 None:intergenic 25.0%
!! TAGAGATAGATTGAATTTTC+TGG + chr3_3:49992750-49992769 None:intergenic 25.0%
! GAAGTTTATGTCTTATGATA+CGG - chr3_3:49992873-49992892 Msa0456420:intron 25.0%
! GAATTGCAATTGATTATAGT+AGG - chr3_3:49992928-49992947 Msa0456420:intron 25.0%
!!! CGGTTTTGTTTATTTCTTTT+TGG - chr3_3:49992973-49992992 Msa0456420:intron 25.0%
! AATAATAAGGTGTAAAGTCA+AGG - chr3_3:49993131-49993150 Msa0456420:intron 25.0%
! ATAATAAGGTGTAAAGTCAA+GGG - chr3_3:49993132-49993151 Msa0456420:intron 25.0%
!!! TTCTCTTCATTTTTCATTCT+AGG - chr3_3:49993305-49993324 Msa0456420:intron 25.0%
! TTTAACCCAAAAGTTTATGT+AGG + chr3_3:49993470-49993489 None:intergenic 25.0%
! CGAAAAGTTGACATATTTAT+AGG + chr3_3:49993938-49993957 None:intergenic 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG + chr3_3:49993908-49993927 None:intergenic 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG + chr3_3:49993907-49993926 None:intergenic 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG - chr3_3:49993907-49993926 Msa0456420:intron 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG - chr3_3:49993908-49993927 Msa0456420:intron 25.0%
! CGAAAAGTTGACATATTTAT+AGG - chr3_3:49993938-49993957 Msa0456420:intron 25.0%
! CGTTGGAAATTAAAAAGTTT+AGG + chr3_3:49993983-49994002 None:intergenic 25.0%
! TGTAAGGACATGTTAAAAAT+AGG - chr3_3:49994010-49994029 Msa0456420:intron 25.0%
! GCATATATAAAGTTAAGGTT+TGG - chr3_3:49994467-49994486 Msa0456420:CDS 25.0%
! GACAACATTAAAATCAACTT+AGG - chr3_3:49994565-49994584 Msa0456420:five_prime_UTR 25.0%
! TCAACTTAGGAAAGAATATT+TGG - chr3_3:49994578-49994597 Msa0456420:five_prime_UTR 25.0%
! GGAAATGAAGATAATGTTTA+TGG + chr3_3:49992428-49992447 None:intergenic 25.0%
! GAAAGTGATGCAAATAATAA+TGG + chr3_3:49992559-49992578 None:intergenic 25.0%
!! TAGAGATAGATTGAATTTTC+TGG + chr3_3:49992750-49992769 None:intergenic 25.0%
! GAAGTTTATGTCTTATGATA+CGG - chr3_3:49992873-49992892 Msa0456420:intron 25.0%
! GAATTGCAATTGATTATAGT+AGG - chr3_3:49992928-49992947 Msa0456420:intron 25.0%
!!! CGGTTTTGTTTATTTCTTTT+TGG - chr3_3:49992973-49992992 Msa0456420:intron 25.0%
! AATAATAAGGTGTAAAGTCA+AGG - chr3_3:49993131-49993150 Msa0456420:intron 25.0%
! ATAATAAGGTGTAAAGTCAA+GGG - chr3_3:49993132-49993151 Msa0456420:intron 25.0%
!!! TTCTCTTCATTTTTCATTCT+AGG - chr3_3:49993305-49993324 Msa0456420:intron 25.0%
! TTTAACCCAAAAGTTTATGT+AGG + chr3_3:49993470-49993489 None:intergenic 25.0%
! CGAAAAGTTGACATATTTAT+AGG + chr3_3:49993938-49993957 None:intergenic 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG + chr3_3:49993908-49993927 None:intergenic 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG + chr3_3:49993907-49993926 None:intergenic 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG - chr3_3:49993907-49993926 Msa0456420:intron 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG - chr3_3:49993908-49993927 Msa0456420:intron 25.0%
! CGAAAAGTTGACATATTTAT+AGG - chr3_3:49993938-49993957 Msa0456420:intron 25.0%
! CGTTGGAAATTAAAAAGTTT+AGG + chr3_3:49993983-49994002 None:intergenic 25.0%
! TGTAAGGACATGTTAAAAAT+AGG - chr3_3:49994010-49994029 Msa0456420:intron 25.0%
! GCATATATAAAGTTAAGGTT+TGG - chr3_3:49994467-49994486 Msa0456420:CDS 25.0%
! GACAACATTAAAATCAACTT+AGG - chr3_3:49994565-49994584 Msa0456420:five_prime_UTR 25.0%
! TCAACTTAGGAAAGAATATT+TGG - chr3_3:49994578-49994597 Msa0456420:five_prime_UTR 25.0%
GAATAACATCTGAATATGAG+TGG + chr3_3:49992506-49992525 None:intergenic 30.0%
AGTGATGCAAATAATAATGG+TGG + chr3_3:49992556-49992575 None:intergenic 30.0%
! ATAAAGGCATATTCAAAGAG+AGG - chr3_3:49992605-49992624 Msa0456420:three_prime_UTR 30.0%
ACTTGTATAATCTTAGGAGA+GGG + chr3_3:49993203-49993222 None:intergenic 30.0%
AACTTGTATAATCTTAGGAG+AGG + chr3_3:49993204-49993223 None:intergenic 30.0%
! GGCATAACTTGTATAATCTT+AGG + chr3_3:49993209-49993228 None:intergenic 30.0%
! TCATAGGAGATTTGAACTTT+TGG + chr3_3:49993389-49993408 None:intergenic 30.0%
! TCCTATGAACTCAAACTTTT+TGG - chr3_3:49993401-49993420 Msa0456420:intron 30.0%
! ACCAAAAAGTTTGAGTTCAT+AGG + chr3_3:49993405-49993424 None:intergenic 30.0%
! TTCTCACCTACATAAACTTT+TGG - chr3_3:49993461-49993480 Msa0456420:intron 30.0%
! TCTCACCTACATAAACTTTT+GGG - chr3_3:49993462-49993481 Msa0456420:intron 30.0%
TTATAGGGACTAAAAGTTGA+AGG + chr3_3:49993894-49993913 None:intergenic 30.0%
TTATAGGGACTAAAAGTTGA+AGG - chr3_3:49993894-49993913 Msa0456420:intron 30.0%
TTTCCAACGATTACTATGTA+AGG - chr3_3:49993994-49994013 Msa0456420:intron 30.0%
TCGCTGCATATATAAAGTTA+AGG - chr3_3:49994462-49994481 Msa0456420:CDS 30.0%
GAATAACATCTGAATATGAG+TGG + chr3_3:49992506-49992525 None:intergenic 30.0%
AGTGATGCAAATAATAATGG+TGG + chr3_3:49992556-49992575 None:intergenic 30.0%
! ATAAAGGCATATTCAAAGAG+AGG - chr3_3:49992605-49992624 Msa0456420:three_prime_UTR 30.0%
ACTTGTATAATCTTAGGAGA+GGG + chr3_3:49993203-49993222 None:intergenic 30.0%
AACTTGTATAATCTTAGGAG+AGG + chr3_3:49993204-49993223 None:intergenic 30.0%
! GGCATAACTTGTATAATCTT+AGG + chr3_3:49993209-49993228 None:intergenic 30.0%
! TCATAGGAGATTTGAACTTT+TGG + chr3_3:49993389-49993408 None:intergenic 30.0%
! TCCTATGAACTCAAACTTTT+TGG - chr3_3:49993401-49993420 Msa0456420:intron 30.0%
! ACCAAAAAGTTTGAGTTCAT+AGG + chr3_3:49993405-49993424 None:intergenic 30.0%
! TTCTCACCTACATAAACTTT+TGG - chr3_3:49993461-49993480 Msa0456420:intron 30.0%
! TCTCACCTACATAAACTTTT+GGG - chr3_3:49993462-49993481 Msa0456420:intron 30.0%
TTATAGGGACTAAAAGTTGA+AGG + chr3_3:49993894-49993913 None:intergenic 30.0%
TTATAGGGACTAAAAGTTGA+AGG - chr3_3:49993894-49993913 Msa0456420:intron 30.0%
TTTCCAACGATTACTATGTA+AGG - chr3_3:49993994-49994013 Msa0456420:intron 30.0%
TCGCTGCATATATAAAGTTA+AGG - chr3_3:49994462-49994481 Msa0456420:CDS 30.0%
CCTCTCGTGTCTTAAAATTT+GGG - chr3_3:49992452-49992471 Msa0456420:three_prime_UTR 35.0%
CTCTCGTGTCTTAAAATTTG+GGG - chr3_3:49992453-49992472 Msa0456420:three_prime_UTR 35.0%
TCTCGTGTCTTAAAATTTGG+GGG - chr3_3:49992454-49992473 Msa0456420:three_prime_UTR 35.0%
CTGCATAGTTCATGCATTAA+AGG - chr3_3:49992663-49992682 Msa0456420:CDS 35.0%
AGACATGTACATCAGTTAGT+CGG - chr3_3:49993077-49993096 Msa0456420:intron 35.0%
TGTCCTTACATAGTAATCGT+TGG + chr3_3:49994000-49994019 None:intergenic 35.0%
TTAGACGAACATTTATCCTC+AGG + chr3_3:49994064-49994083 None:intergenic 35.0%
TATAGGTTATGGACCATCAA+TGG + chr3_3:49994402-49994421 None:intergenic 35.0%
TTAATATCACACACTGCACT+TGG + chr3_3:49994426-49994445 None:intergenic 35.0%
ATGCGTGGGATATAAAAACA+TGG + chr3_3:49994528-49994547 None:intergenic 35.0%
CCTCTCGTGTCTTAAAATTT+GGG - chr3_3:49992452-49992471 Msa0456420:three_prime_UTR 35.0%
CTCTCGTGTCTTAAAATTTG+GGG - chr3_3:49992453-49992472 Msa0456420:three_prime_UTR 35.0%
TCTCGTGTCTTAAAATTTGG+GGG - chr3_3:49992454-49992473 Msa0456420:three_prime_UTR 35.0%
CTGCATAGTTCATGCATTAA+AGG - chr3_3:49992663-49992682 Msa0456420:CDS 35.0%
AGACATGTACATCAGTTAGT+CGG - chr3_3:49993077-49993096 Msa0456420:intron 35.0%
TGTCCTTACATAGTAATCGT+TGG + chr3_3:49994000-49994019 None:intergenic 35.0%
TTAGACGAACATTTATCCTC+AGG + chr3_3:49994064-49994083 None:intergenic 35.0%
TATAGGTTATGGACCATCAA+TGG + chr3_3:49994402-49994421 None:intergenic 35.0%
TTAATATCACACACTGCACT+TGG + chr3_3:49994426-49994445 None:intergenic 35.0%
ATGCGTGGGATATAAAAACA+TGG + chr3_3:49994528-49994547 None:intergenic 35.0%
!!! TTTTAAGACACGAGAGGCAA+TGG + chr3_3:49992449-49992468 None:intergenic 40.0%
GCCTCTCGTGTCTTAAAATT+TGG - chr3_3:49992451-49992470 Msa0456420:three_prime_UTR 40.0%
!!! CCCAAATTTTAAGACACGAG+AGG + chr3_3:49992455-49992474 None:intergenic 40.0%
AAGGCATATTCAAAGAGAGG+AGG - chr3_3:49992608-49992627 Msa0456420:three_prime_UTR 40.0%
! TTTCTGGTCAAGCTCTTTGA+GGG + chr3_3:49992734-49992753 None:intergenic 40.0%
!! TTTTCTGGTCAAGCTCTTTG+AGG + chr3_3:49992735-49992754 None:intergenic 40.0%
AGTTTCCTCTCCAAAACTCA+AGG - chr3_3:49992777-49992796 Msa0456420:CDS 40.0%
GTTTCCTCTCCAAAACTCAA+GGG - chr3_3:49992778-49992797 Msa0456420:CDS 40.0%
ACAAAATCCTTCGTCTAACG+AGG + chr3_3:49992899-49992918 None:intergenic 40.0%
TGTAACAACGAGTGCAAAGT+AGG + chr3_3:49993019-49993038 None:intergenic 40.0%
CTTTGCACTCGTTGTTACAA+TGG - chr3_3:49993020-49993039 Msa0456420:intron 40.0%
!! AGTAACTTCTGAAGTACCTG+AGG - chr3_3:49994045-49994064 Msa0456420:intron 40.0%
GAGGTGATAAGTGACAACAA+TGG - chr3_3:49994275-49994294 Msa0456420:CDS 40.0%
!! TGGCTGGCTTAATTACAGTA+AGG + chr3_3:49994382-49994401 None:intergenic 40.0%
GTAATTAAGCCAGCCATTGA+TGG - chr3_3:49994386-49994405 Msa0456420:CDS 40.0%
CTGCACTTGGATATAGGTTA+TGG + chr3_3:49994413-49994432 None:intergenic 40.0%
AGAATCTTAGCAACATGCGT+GGG + chr3_3:49994542-49994561 None:intergenic 40.0%
!!! TTTTAAGACACGAGAGGCAA+TGG + chr3_3:49992449-49992468 None:intergenic 40.0%
GCCTCTCGTGTCTTAAAATT+TGG - chr3_3:49992451-49992470 Msa0456420:three_prime_UTR 40.0%
!!! CCCAAATTTTAAGACACGAG+AGG + chr3_3:49992455-49992474 None:intergenic 40.0%
AAGGCATATTCAAAGAGAGG+AGG - chr3_3:49992608-49992627 Msa0456420:three_prime_UTR 40.0%
! TTTCTGGTCAAGCTCTTTGA+GGG + chr3_3:49992734-49992753 None:intergenic 40.0%
!! TTTTCTGGTCAAGCTCTTTG+AGG + chr3_3:49992735-49992754 None:intergenic 40.0%
AGTTTCCTCTCCAAAACTCA+AGG - chr3_3:49992777-49992796 Msa0456420:CDS 40.0%
GTTTCCTCTCCAAAACTCAA+GGG - chr3_3:49992778-49992797 Msa0456420:CDS 40.0%
ACAAAATCCTTCGTCTAACG+AGG + chr3_3:49992899-49992918 None:intergenic 40.0%
TGTAACAACGAGTGCAAAGT+AGG + chr3_3:49993019-49993038 None:intergenic 40.0%
CTTTGCACTCGTTGTTACAA+TGG - chr3_3:49993020-49993039 Msa0456420:intron 40.0%
!! AGTAACTTCTGAAGTACCTG+AGG - chr3_3:49994045-49994064 Msa0456420:intron 40.0%
GAGGTGATAAGTGACAACAA+TGG - chr3_3:49994275-49994294 Msa0456420:CDS 40.0%
!! TGGCTGGCTTAATTACAGTA+AGG + chr3_3:49994382-49994401 None:intergenic 40.0%
GTAATTAAGCCAGCCATTGA+TGG - chr3_3:49994386-49994405 Msa0456420:CDS 40.0%
CTGCACTTGGATATAGGTTA+TGG + chr3_3:49994413-49994432 None:intergenic 40.0%
AGAATCTTAGCAACATGCGT+GGG + chr3_3:49994542-49994561 None:intergenic 40.0%
! AAGCTCTTTGAGGGCTTCTT+TGG + chr3_3:49992725-49992744 None:intergenic 45.0%
!!! AGTACCCTTGAGTTTTGGAG+AGG + chr3_3:49992785-49992804 None:intergenic 45.0%
TCTAGGAATTGACACTCGCT+CGG - chr3_3:49993322-49993341 Msa0456420:intron 45.0%
TCCTTTCTTGCACTTCTAGC+TGG - chr3_3:49994317-49994336 Msa0456420:CDS 45.0%
! ACCAGCTAGAAGTGCAAGAA+AGG + chr3_3:49994321-49994340 None:intergenic 45.0%
! TTTCTTGCACTTCTAGCTGG+TGG - chr3_3:49994320-49994339 Msa0456420:CDS 45.0%
CACACACTGCACTTGGATAT+AGG + chr3_3:49994419-49994438 None:intergenic 45.0%
CAGAATCTTAGCAACATGCG+TGG + chr3_3:49994543-49994562 None:intergenic 45.0%
! AAGCTCTTTGAGGGCTTCTT+TGG + chr3_3:49992725-49992744 None:intergenic 45.0%
!!! AGTACCCTTGAGTTTTGGAG+AGG + chr3_3:49992785-49992804 None:intergenic 45.0%
TCTAGGAATTGACACTCGCT+CGG - chr3_3:49993322-49993341 Msa0456420:intron 45.0%
TCCTTTCTTGCACTTCTAGC+TGG - chr3_3:49994317-49994336 Msa0456420:CDS 45.0%
! ACCAGCTAGAAGTGCAAGAA+AGG + chr3_3:49994321-49994340 None:intergenic 45.0%
! TTTCTTGCACTTCTAGCTGG+TGG - chr3_3:49994320-49994339 Msa0456420:CDS 45.0%
CACACACTGCACTTGGATAT+AGG + chr3_3:49994419-49994438 None:intergenic 45.0%
CAGAATCTTAGCAACATGCG+TGG + chr3_3:49994543-49994562 None:intergenic 45.0%
!!! ATGAATTTTTATATTTTTTA+CGG - chr3_3:49993720-49993739 Msa0456420:intron 5.0%
!!! AAAAAATTCTAAATTTTTTT+AGG - chr3_3:49993774-49993793 Msa0456420:intron 5.0%
!! ACAAAAAATAAATTAAAAAT+AGG - chr3_3:49993847-49993866 Msa0456420:intron 5.0%
!! CAAAAAATAAATTAAAAATA+GGG - chr3_3:49993848-49993867 Msa0456420:intron 5.0%
!!! ATGAATTTTTATATTTTTTA+CGG - chr3_3:49993720-49993739 Msa0456420:intron 5.0%
!!! AAAAAATTCTAAATTTTTTT+AGG - chr3_3:49993774-49993793 Msa0456420:intron 5.0%
!! ACAAAAAATAAATTAAAAAT+AGG - chr3_3:49993847-49993866 Msa0456420:intron 5.0%
!! CAAAAAATAAATTAAAAATA+GGG - chr3_3:49993848-49993867 Msa0456420:intron 5.0%
! GGGAGTGTAGTGTAGAGTGA+AGG - chr3_3:49992474-49992493 Msa0456420:three_prime_UTR 50.0%
!! GGGCTTCTTTGGCTTTCTCT+GGG + chr3_3:49992714-49992733 None:intergenic 50.0%
!! AGGGCTTCTTTGGCTTTCTC+TGG + chr3_3:49992715-49992734 None:intergenic 50.0%
!!! CGGAGAGTACCCTTGAGTTT+TGG + chr3_3:49992790-49992809 None:intergenic 50.0%
AGAGATGAAGCCTACGAGAG+AGG - chr3_3:49994253-49994272 Msa0456420:intron 50.0%
CTTATCACCTCCTCTCTCGT+AGG + chr3_3:49994266-49994285 None:intergenic 50.0%
GGTTATGGACCATCAATGGC+TGG + chr3_3:49994398-49994417 None:intergenic 50.0%
! GGGAGTGTAGTGTAGAGTGA+AGG - chr3_3:49992474-49992493 Msa0456420:three_prime_UTR 50.0%
!! GGGCTTCTTTGGCTTTCTCT+GGG + chr3_3:49992714-49992733 None:intergenic 50.0%
!! AGGGCTTCTTTGGCTTTCTC+TGG + chr3_3:49992715-49992734 None:intergenic 50.0%
!!! CGGAGAGTACCCTTGAGTTT+TGG + chr3_3:49992790-49992809 None:intergenic 50.0%
AGAGATGAAGCCTACGAGAG+AGG - chr3_3:49994253-49994272 Msa0456420:intron 50.0%
CTTATCACCTCCTCTCTCGT+AGG + chr3_3:49994266-49994285 None:intergenic 50.0%
GGTTATGGACCATCAATGGC+TGG + chr3_3:49994398-49994417 None:intergenic 50.0%
GGTGGCTGCTTGATGCTAAG+AGG + chr3_3:49992538-49992557 None:intergenic 55.0%
GATACGGCCTCGTTAGACGA+AGG - chr3_3:49992889-49992908 Msa0456420:intron 55.0%
GATGAAGCCTACGAGAGAGG+AGG - chr3_3:49994256-49994275 Msa0456420:intron 55.0%
GGTGGCTGCTTGATGCTAAG+AGG + chr3_3:49992538-49992557 None:intergenic 55.0%
GATACGGCCTCGTTAGACGA+AGG - chr3_3:49992889-49992908 Msa0456420:intron 55.0%
GATGAAGCCTACGAGAGAGG+AGG - chr3_3:49994256-49994275 Msa0456420:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 49992412 49994653 49992412 ID=Msa0456420;Name=Msa0456420
chr3_3 mRNA 49992412 49994653 49992412 ID=Msa0456420-mRNA-1;Parent=Msa0456420;Name=Msa0456420-mRNA-1;_AED=0.00;_eAED=0.00;_QI=105|1|1|1|1|1|2|238|149
chr3_3 exon 49992412 49992816 49992412 ID=Msa0456420-mRNA-1:exon:9577;Parent=Msa0456420-mRNA-1
chr3_3 exon 49994266 49994653 49994266 ID=Msa0456420-mRNA-1:exon:9576;Parent=Msa0456420-mRNA-1
chr3_3 five_prime_UTR 49994549 49994653 49994549 ID=Msa0456420-mRNA-1:five_prime_utr;Parent=Msa0456420-mRNA-1
chr3_3 CDS 49994266 49994548 49994266 ID=Msa0456420-mRNA-1:cds;Parent=Msa0456420-mRNA-1
chr3_3 CDS 49992650 49992816 49992650 ID=Msa0456420-mRNA-1:cds;Parent=Msa0456420-mRNA-1
chr3_3 three_prime_UTR 49992412 49992649 49992412 ID=Msa0456420-mRNA-1:three_prime_utr;Parent=Msa0456420-mRNA-1
Gene Sequence

>Msa0456420

ATGTTATTCTCTATGAAACCTCTTAGCATCAAGCAGCCACCATTATTATTTGCATCACTTTCTAAACCGTTATTATTACATAATAATGATAAAGGCATATTCAAAGAGAGGAGGCTCATAAAATATTCTTCTTCATTTAATTCATGCTGCATAGTTCATGCATTAAAGGAAGATTCAAAGCAATACGAGATAGACCCAGAGAAAGCCAAAGAAGCCCTCAAAGAGCTTGACCAGAAAATTCAATCTATCTCTAGTAGAGAAGTTTCCTCTCCAAAACTCAAGGTTTCAGAGATGAAGCCTACGAGAGAGGAGGTGATAAGTGACAACAATGGTAAGCTAGAAATTTCAGAGTCCTTTCTTGCACTTCTAGCTGGTGGACTTGTTCTCTTTACTATATTGTACAATGTACTATTCCTTACTGTAATTAAGCCAGCCATTGATGGTCCATAA
Protein sequence

>Msa0456420

MLFSMKPLSIKQPPLLFASLSKPLLLHNNDKGIFKERRLIKYSSSFNSCCIVHALKEDSKQYEIDPEKAKEALKELDQKIQSISSREVSSPKLKVSEMKPTREEVISDNNGKLEISESFLALLAGGLVLFTILYNVLFLTVIKPAIDGP*