AlfalfaGEDB | Msa0456420

M. sativa cultivar zhongmu-4 / Msa0456420

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456420	XP_003600464.2	94.000	150	7	2	1	149	1	149	6.79e-84	253

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456420	I3T0P4	94.000	150	7	2	1	149	1	149	3.25e-84	253

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0094990	Msa0456420	0.805858	1.089666e-49	-8.615850e-47
Msa0370690	Msa0456420	0.800214	1.600689e-48	-8.615850e-47
Msa0456420	Msa0465640	0.827355	1.637840e-54	-8.615850e-47
Msa0456420	Msa0572820	0.804996	1.651720e-49	-8.615850e-47
Msa0456420	Msa0679780	0.808757	2.647596e-50	-8.615850e-47
Msa0456420	Msa1011060	0.805585	1.243245e-49	-8.615850e-47
Msa0456420	Msa1098340	0.808397	3.160714e-50	-8.615850e-47
Msa0456420	Msa1422150	0.807492	4.923622e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456420	MtrunA17_Chr3g0103371	94.000	150	7	2	1	149	1	149	6.25e-88	253

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456420	AT2G01870.1	42.453	106	45	5	51	149	30	126	1.45e-15	69.7

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Find 44 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCTCTTTGAGGGCTTCTT+TGG	0.197880	3_3:+49994321	None:intergenic
AAGGCATATTCAAAGAGAGG+AGG	0.658459	3_3:-49994435	Msa0456420:CDS
ACAATTGATGAAAATTTAAG+TGG	0.411884	3_3:+49992440	None:intergenic
ACCAGCTAGAAGTGCAAGAA+AGG	0.499982	3_3:+49992725	None:intergenic
AGAATCTTAGCAACATGCGT+GGG	0.645379	3_3:+49992504	None:intergenic
AGAGATGAAGCCTACGAGAG+AGG	0.636132	3_3:-49992790	Msa0456420:CDS
AGGGCTTCTTTGGCTTTCTC+TGG	0.231668	3_3:+49994331	None:intergenic
AGTGATGCAAATAATAATGG+TGG	0.676828	3_3:+49994490	None:intergenic
AGTTTCCTCTCCAAAACTCA+AGG	0.567427	3_3:-49994266	Msa0456420:CDS
ATAAAGGCATATTCAAAGAG+AGG	0.608157	3_3:-49994438	Msa0456420:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATAACATCTGAATATGAG+TGG	+	chr3_3:49992506-49992525	None:intergenic	30.0%
AGTGATGCAAATAATAATGG+TGG	+	chr3_3:49992556-49992575	None:intergenic	30.0%
ACTTGTATAATCTTAGGAGA+GGG	+	chr3_3:49993203-49993222	None:intergenic	30.0%
AACTTGTATAATCTTAGGAG+AGG	+	chr3_3:49993204-49993223	None:intergenic	30.0%
TTATAGGGACTAAAAGTTGA+AGG	+	chr3_3:49993894-49993913	None:intergenic	30.0%
TTATAGGGACTAAAAGTTGA+AGG	-	chr3_3:49993894-49993913	Msa0456420:intron	30.0%
TTTCCAACGATTACTATGTA+AGG	-	chr3_3:49993994-49994013	Msa0456420:intron	30.0%
TCGCTGCATATATAAAGTTA+AGG	-	chr3_3:49994462-49994481	Msa0456420:CDS	30.0%
GAATAACATCTGAATATGAG+TGG	+	chr3_3:49992506-49992525	None:intergenic	30.0%
AGTGATGCAAATAATAATGG+TGG	+	chr3_3:49992556-49992575	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	49992412	49994653	49992412	ID=Msa0456420;Name=Msa0456420
chr3_3	mRNA	49992412	49994653	49992412	ID=Msa0456420-mRNA-1;Parent=Msa0456420;Name=Msa0456420-mRNA-1;_AED=0.00;_eAED=0.00;_QI=105\|1\|1\|1\|1\|1\|2\|238\|149
chr3_3	exon	49992412	49992816	49992412	ID=Msa0456420-mRNA-1:exon:9577;Parent=Msa0456420-mRNA-1
chr3_3	exon	49994266	49994653	49994266	ID=Msa0456420-mRNA-1:exon:9576;Parent=Msa0456420-mRNA-1
chr3_3	five_prime_UTR	49994549	49994653	49994549	ID=Msa0456420-mRNA-1:five_prime_utr;Parent=Msa0456420-mRNA-1
chr3_3	CDS	49994266	49994548	49994266	ID=Msa0456420-mRNA-1:cds;Parent=Msa0456420-mRNA-1
chr3_3	CDS	49992650	49992816	49992650	ID=Msa0456420-mRNA-1:cds;Parent=Msa0456420-mRNA-1
chr3_3	three_prime_UTR	49992412	49992649	49992412	ID=Msa0456420-mRNA-1:three_prime_utr;Parent=Msa0456420-mRNA-1

Gene Sequence

>Msa0456420

ATGTTATTCTCTATGAAACCTCTTAGCATCAAGCAGCCACCATTATTATTTGCATCACTTTCTAAACCGTTATTATTACATAATAATGATAAAGGCATATTCAAAGAGAGGAGGCTCATAAAATATTCTTCTTCATTTAATTCATGCTGCATAGTTCATGCATTAAAGGAAGATTCAAAGCAATACGAGATAGACCCAGAGAAAGCCAAAGAAGCCCTCAAAGAGCTTGACCAGAAAATTCAATCTATCTCTAGTAGAGAAGTTTCCTCTCCAAAACTCAAGGTTTCAGAGATGAAGCCTACGAGAGAGGAGGTGATAAGTGACAACAATGGTAAGCTAGAAATTTCAGAGTCCTTTCTTGCACTTCTAGCTGGTGGACTTGTTCTCTTTACTATATTGTACAATGTACTATTCCTTACTGTAATTAAGCCAGCCATTGATGGTCCATAA

Protein sequence

>Msa0456420

MLFSMKPLSIKQPPLLFASLSKPLLLHNNDKGIFKERRLIKYSSSFNSCCIVHALKEDSKQYEIDPEKAKEALKELDQKIQSISSREVSSPKLKVSEMKPTREEVISDNNGKLEISESFLALLAGGLVLFTILYNVLFLTVIKPAIDGP*