AlfalfaGEDB | Msa0464810

M. sativa cultivar zhongmu-4 / Msa0464810

KEGG: K16292
GO: GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005788 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009505 GO:0009555 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009791 GO:0009908 GO:0009987 GO:0010623 GO:0012501 GO:0012505 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0030312 GO:0031974 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0070013 GO:0071704 GO:0071944 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575
PFAM: PF08246.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0464810	XP_013460973.1	96.961	362	11	0	1	362	1	362	0.0	740

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0464810	sp\|P25803\|CYSEP_PHAVU	82.044	362	65	0	1	362	1	362	0.0	633

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0464810	G8A2F7	96.961	362	11	0	1	362	1	362	0.0	740

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0196860	Msa0464810	0.806766	7.014274e-50	-8.615850e-47
Msa0418930	Msa0464810	0.872426	3.210786e-67	-8.615850e-47
Msa0464810	Msa0702720	0.809154	2.176980e-50	-8.615850e-47
Msa0464810	Msa1338370	0.800853	1.185859e-48	-8.615850e-47
Msa0464810	Msa1341840	0.810440	1.151458e-50	-8.615850e-47
Msa0464810	Msa1435360	0.964324	3.488571e-123	-8.615850e-47
Msa0464810	Msa1461370	0.821759	3.403825e-53	-8.615850e-47
Msa0276400	Msa0464810	0.807549	4.787086e-50	-8.615850e-47
Msa0316440	Msa0464810	0.803554	3.298446e-49	-8.615850e-47
Msa0339130	Msa0464810	0.820252	7.567539e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0464810	MtrunA17_Chr3g0116551	96.961	362	11	0	1	362	1	362	0.0	740
Msa0464810	MtrunA17_Chr4g0058081	76.243	362	84	2	1	362	1	360	0.0	593
Msa0464810	MtrunA17_Chr4g0041481	57.692	312	121	7	35	343	35	338	4.84e-125	363
Msa0464810	MtrunA17_Chr4g0041521	57.051	312	123	7	35	343	35	338	1.98e-124	361
Msa0464810	MtrunA17_Chr4g0060251	54.890	317	127	5	35	343	35	343	1.90e-123	359
Msa0464810	MtrunA17_Chr5g0405751	55.031	318	129	6	33	343	32	342	3.69e-123	358
Msa0464810	MtrunA17_Chr4g0041061	51.724	348	158	7	1	343	1	343	8.84e-123	357
Msa0464810	MtrunA17_Chr4g0024411	57.325	314	123	7	38	344	33	342	9.32e-123	357
Msa0464810	MtrunA17_Chr4g0040881	54.517	321	137	6	28	344	29	344	1.04e-122	357
Msa0464810	MtrunA17_Chr3g0144281	51.289	349	159	5	3	345	5	348	5.55e-122	355
Msa0464810	MtrunA17_Chr1g0208261	55.215	326	141	5	23	345	73	396	8.76e-122	361
Msa0464810	MtrunA17_Chr4g0053311	51.320	341	151	5	8	345	14	342	1.32e-121	355
Msa0464810	MtrunA17_Chr4g0041081	52.615	325	137	6	28	344	29	344	7.72e-121	352
Msa0464810	MtrunA17_Chr3g0134231	51.989	352	156	8	6	345	12	362	3.55e-120	355
Msa0464810	MtrunA17_Chr2g0315491	53.488	344	145	7	5	343	6	339	3.96e-118	345
Msa0464810	MtrunA17_Chr4g0040851	50.585	342	157	6	6	344	27	359	7.68e-117	343
Msa0464810	MtrunA17_Chr2g0325121	50.000	348	162	5	1	346	1	338	2.24e-115	338
Msa0464810	MtrunA17_Chr4g0041471	51.466	307	145	3	40	344	37	341	3.21e-112	330
Msa0464810	MtrunA17_Chr1g0153011	50.143	349	146	8	6	346	16	344	4.81e-110	329
Msa0464810	MtrunA17_Chr2g0319991	46.608	339	170	5	10	344	12	343	1.00e-105	314
Msa0464810	MtrunA17_Chr7g0248391	46.483	327	158	7	25	347	27	340	1.76e-99	298
Msa0464810	MtrunA17_Chr7g0248811	45.376	346	174	7	1	343	1	334	1.74e-98	295
Msa0464810	MtrunA17_Chr3g0142071	48.875	311	156	3	39	347	49	358	5.52e-98	298
Msa0464810	MtrunA17_Chr2g0283901	43.939	330	167	11	25	345	36	356	2.49e-83	261
Msa0464810	MtrunA17_Chr8g0379151	43.380	355	175	13	6	345	30	373	4.82e-76	239
Msa0464810	MtrunA17_Chr2g0283741	40.116	344	186	12	27	361	41	373	1.54e-73	233
Msa0464810	MtrunA17_Chr0c27g0493921	43.960	298	147	9	53	344	65	348	1.40e-72	229
Msa0464810	MtrunA17_Chr2g0283671	52.315	216	100	3	129	344	15	227	1.45e-69	217
Msa0464810	MtrunA17_Chr4g0070441	43.902	287	143	9	53	331	65	341	9.41e-69	219
Msa0464810	MtrunA17_Chr2g0311381	40.606	330	154	16	40	343	36	349	8.37e-64	207
Msa0464810	MtrunA17_Chr1g0155781	40.468	299	150	9	54	336	66	352	4.77e-63	205
Msa0464810	MtrunA17_Chr2g0311471	41.860	301	158	11	55	344	52	346	6.05e-62	202
Msa0464810	MtrunA17_Chr4g0035551	38.127	299	156	8	54	336	63	348	3.08e-59	195
Msa0464810	MtrunA17_Chr2g0311391	38.484	343	161	13	40	342	36	368	1.67e-58	194
Msa0464810	MtrunA17_Chr1g0156161	42.731	227	122	6	119	342	40	261	8.21e-57	186
Msa0464810	MtrunA17_Chr1g0199131	31.965	341	197	10	15	336	34	358	9.42e-53	179
Msa0464810	MtrunA17_Chr7g0248421	52.229	157	70	3	152	307	57	209	4.69e-44	151
Msa0464810	MtrunA17_Chr2g0283661	31.429	315	144	8	32	343	25	270	9.06e-44	152
Msa0464810	MtrunA17_Chr4g0036731	34.965	286	147	7	54	336	63	312	1.76e-43	153
Msa0464810	MtrunA17_Chr7g0248371	63.636	99	35	1	246	344	34	131	2.22e-42	145
Msa0464810	MtrunA17_Chr2g0283751	36.082	291	150	13	6	274	10	286	1.04e-41	147
Msa0464810	MtrunA17_Chr4g0036281	34.021	291	147	9	54	336	63	316	7.96e-41	146
Msa0464810	MtrunA17_Chr4g0037291	34.021	291	147	9	54	336	104	357	1.29e-40	147
Msa0464810	MtrunA17_Chr7g0248661	64.000	75	27	0	270	344	2	76	5.94e-32	115
Msa0464810	MtrunA17_Chr2g0311091	39.259	135	71	2	55	178	50	184	1.06e-24	99.4
Msa0464810	MtrunA17_Chr7g0271041	27.174	276	165	11	86	336	66	330	7.68e-24	100
Msa0464810	MtrunA17_Chr1g0156771	47.525	101	49	3	127	224	54	153	7.72e-24	96.3
Msa0464810	MtrunA17_Chr7g0271031	30.328	244	138	10	130	350	153	387	6.98e-23	99.0
Msa0464810	MtrunA17_Chr8g0379671	38.462	130	69	5	169	296	23	143	4.59e-16	75.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0464810	AT5G50260.1	70.278	360	106	1	4	362	2	361	0.0	556
Msa0464810	AT3G48340.1	67.036	361	116	2	4	362	2	361	0.0	516
Msa0464810	AT3G48350.1	64.167	360	127	2	4	362	2	360	5.15e-177	496
Msa0464810	AT3G48350.2	65.580	276	93	2	5	278	2	277	3.76e-134	385
Msa0464810	AT1G20850.1	54.348	322	143	3	25	345	36	354	3.59e-122	357
Msa0464810	AT4G35350.1	51.289	349	160	4	3	345	9	353	1.08e-121	355
Msa0464810	AT1G47128.1	49.280	347	165	4	6	346	13	354	7.86e-118	349
Msa0464810	AT5G43060.1	50.571	350	157	5	6	345	11	354	8.47e-118	349
Msa0464810	AT3G19390.1	48.159	353	174	6	1	350	5	351	2.80e-115	342
Msa0464810	AT5G45890.1	47.143	350	167	7	6	343	1	344	1.84e-114	337
Msa0464810	AT4G36880.1	51.064	329	150	6	32	351	41	367	1.76e-113	335
Msa0464810	AT3G19400.1	51.592	314	142	7	39	347	43	351	1.63e-107	319
Msa0464810	AT1G06260.1	48.000	350	166	7	1	345	5	343	6.38e-106	315
Msa0464810	AT4G11310.1	46.518	359	171	8	3	347	4	355	3.62e-105	313
Msa0464810	AT4G11320.2	46.218	357	170	8	6	347	13	362	5.34e-103	308
Msa0464810	AT4G11320.1	46.218	357	170	8	6	347	13	362	5.34e-103	308
Msa0464810	AT1G09850.1	49.355	310	150	4	38	345	30	334	1.64e-99	301
Msa0464810	AT2G27420.1	45.821	347	173	5	6	344	8	347	1.83e-99	298
Msa0464810	AT4G23520.1	42.458	358	190	7	3	350	4	355	1.98e-98	296
Msa0464810	AT3G49340.1	44.412	340	182	5	9	344	4	340	5.80e-98	294
Msa0464810	AT2G34080.1	44.638	345	180	6	6	344	5	344	1.48e-94	286
Msa0464810	AT1G29090.1	45.000	360	167	11	1	344	10	354	1.67e-91	278
Msa0464810	AT3G43960.1	46.154	325	159	11	39	354	40	357	1.78e-88	271
Msa0464810	AT1G29080.1	41.595	351	192	8	1	344	1	345	3.96e-88	270
Msa0464810	AT4G35350.2	48.936	282	134	4	3	278	9	286	3.52e-87	265
Msa0464810	AT1G29110.1	39.316	351	188	9	1	344	1	333	7.70e-79	245
Msa0464810	AT3G19400.2	50.417	240	110	6	39	273	43	278	1.95e-72	227
Msa0464810	AT5G60360.1	41.667	300	155	9	51	344	71	356	2.56e-69	221
Msa0464810	AT3G45310.1	41.060	302	154	10	51	344	71	356	5.10e-67	216
Msa0464810	AT5G60360.2	41.333	300	155	10	51	344	71	355	9.44e-67	215
Msa0464810	AT3G45310.2	40.728	302	154	11	51	344	71	355	2.01e-64	209
Msa0464810	AT5G60360.3	40.550	291	153	9	51	335	71	347	3.52e-64	208
Msa0464810	AT2G21430.1	33.903	351	198	10	6	336	13	349	1.02e-60	199
Msa0464810	AT4G39090.1	37.124	299	160	8	54	336	66	352	2.91e-57	191
Msa0464810	AT3G54940.2	37.793	299	160	9	54	336	66	354	3.33e-55	185
Msa0464810	AT4G16190.1	37.584	298	160	9	55	336	71	358	1.30e-53	181
Msa0464810	AT3G54940.3	37.674	215	122	4	54	260	66	276	4.43e-39	140
Msa0464810	AT1G02305.1	27.893	337	208	11	50	362	33	358	6.29e-28	112
Msa0464810	AT4G01610.1	27.660	329	195	15	50	350	30	343	1.14e-23	100
Msa0464810	AT4G01610.2	27.052	329	197	15	50	350	30	343	9.17e-22	95.5
Msa0464810	AT1G02300.1	31.799	239	131	10	147	362	146	375	4.08e-21	94.0
Msa0464810	AT2G22160.1	46.465	99	49	1	53	151	8	102	9.23e-21	86.7

Find 116 sgRNAs with CRISPR-Local

Find 296 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAGCCTATTTGTCTTTATT+TGG	0.139485	3_3:+65009078	None:intergenic
CCGTTGATGGAACTAATTAT+TGG	0.225728	3_3:-65008429	Msa0464810:CDS
TTTGATGACAACGTATGATT+TGG	0.286950	3_3:-65008126	Msa0464810:three_prime_UTR
ATCAGATCCTCCGGCATCTA+TGG	0.300046	3_3:+65008695	None:intergenic
AGTTTCCATGTTTCTTTGTT+TGG	0.301765	3_3:+65009907	None:intergenic
ACATGACCAACCATGAATTT+AGG	0.309976	3_3:-65009628	Msa0464810:CDS
AAGCTCCTGCTTCTGTTGAT+TGG	0.324173	3_3:-65009514	Msa0464810:CDS
GAACTCATGGGGAGCGGAAT+GGG	0.328427	3_3:-65008398	Msa0464810:CDS
ATGTTTGTGTTGTTCAAGTA+TGG	0.330691	3_3:+65010107	None:intergenic
TTTGAGTACATCAAGCAAAA+AGG	0.333516	3_3:-65008975	Msa0464810:CDS
CCGAGGACACATCTTTATCT+TGG	0.334739	3_3:+65009819	None:intergenic
TAAACCAAATAAAGACAAAT+AGG	0.344680	3_3:-65009082	Msa0464810:CDS
TGTCCTGTATAGGTAGTTGC+TGG	0.345293	3_3:-65009136	Msa0464810:intron
TCAAATATTTAAAGTTCATA+AGG	0.354352	3_3:+65008157	None:intergenic
AATGATGGAGATGATAAAAT+AGG	0.371330	3_3:+65010082	None:intergenic
TGAATGGAAGTTGGATGAAA+AGG	0.372453	3_3:+65009960	None:intergenic
GCATCTATGGCTACGGATAC+AGG	0.385812	3_3:+65008708	None:intergenic
TCAACTGTTGTAGCTGTTGA+AGG	0.388321	3_3:-65009107	Msa0464810:CDS
TTGTTAAGTTTCAACTTGTA+AGG	0.389898	3_3:+65009659	None:intergenic
AGGAGGTTAAAGGTTTCTTA+AGG	0.390380	3_3:+65010044	None:intergenic
AGGATGCAATGGTGGGCTAA+TGG	0.392108	3_3:-65009006	Msa0464810:CDS
TTTCGAATGTATTAATTGAA+TGG	0.396921	3_3:+65009944	None:intergenic
GATCCATCATTTGCTGTGTA+AGG	0.397020	3_3:+65008927	None:intergenic
GCAGGGGATGGTTTATATAG+AGG	0.401101	3_3:+65009989	None:intergenic
CACTTCCACGTGGCGTGCCT+CGG	0.411657	3_3:+65009564	None:intergenic
GATTGTTCTGTCCATTGCTT+TGG	0.412192	3_3:-65009870	Msa0464810:CDS
AACATTCATTCAAATATCAA+TGG	0.413261	3_3:-65007998	Msa0464810:three_prime_UTR
GGAGGTTAAAGGTTTCTTAA+GGG	0.416407	3_3:+65010045	None:intergenic
ATCAACAGAAGCAGGAGCTT+TGG	0.424537	3_3:+65009517	None:intergenic
TGACCATCAATTGACACAGC+TGG	0.433204	3_3:+65008786	None:intergenic
TGTATTAATTGAATGGAAGT+TGG	0.441659	3_3:+65009951	None:intergenic
GAATGGAAGTTGGATGAAAA+GGG	0.443264	3_3:+65009961	None:intergenic
GGTGTAGCAATAGTAGGATA+TGG	0.443941	3_3:-65008457	Msa0464810:CDS
TGATGGTCATGAGACTGTTC+CGG	0.449890	3_3:-65008772	Msa0464810:CDS
GGAGTCACCACACGGTTTCT+CGG	0.450148	3_3:-65009763	Msa0464810:CDS
GGATATGGAACAACCGTTGA+TGG	0.454887	3_3:-65008442	Msa0464810:CDS
GTCCCAGCTGTGTCAATTGA+TGG	0.458384	3_3:-65008789	Msa0464810:CDS
CGAGGCACGCCACGTGGAAG+TGG	0.460548	3_3:-65009563	Msa0464810:CDS
TCATCCTTGGTAGATGATAA+AGG	0.462977	3_3:+65008262	None:intergenic
GGATGGTTTATATAGAGGAT+TGG	0.465171	3_3:+65009994	None:intergenic
TTTCTCCAATCAACAGAAGC+AGG	0.466214	3_3:+65009509	None:intergenic
TATCCATCTTGTTAGTGTTG+TGG	0.468486	3_3:+65009684	None:intergenic
CATAGGTAGTCCTAAATTCA+TGG	0.476124	3_3:+65009618	None:intergenic
CATAATGGTTGCAGGGAATG+AGG	0.484219	3_3:+65008027	None:intergenic
TGGATGAAAAGGGAAAGAGC+AGG	0.491370	3_3:+65009971	None:intergenic
ATTGGATTGTGAGGAACTCA+TGG	0.493389	3_3:-65008411	Msa0464810:CDS
CTATTACAGGGAGTATTTAG+TGG	0.493552	3_3:-65008505	Msa0464810:intron
AATCATGGTGTAGCAATAGT+AGG	0.495226	3_3:-65008463	Msa0464810:CDS
GTCACTGAGGTCAAGGATCA+AGG	0.495703	3_3:-65009476	Msa0464810:CDS
TCTAACAAGGAAGGACTTTG+TGG	0.498260	3_3:-65008340	Msa0464810:CDS
TTATATAGAGGATTGGAGAA+AGG	0.498533	3_3:+65010001	None:intergenic
GAGGTTAAAGGTTTCTTAAG+GGG	0.504781	3_3:+65010046	None:intergenic
GTAGATGATAAAGGTCCTGC+TGG	0.507201	3_3:+65008271	None:intergenic
AAACATTCAAAGCTCATCCT+TGG	0.508057	3_3:+65008249	None:intergenic
GGATGAAAAGGGAAAGAGCA+GGG	0.510179	3_3:+65009972	None:intergenic
TACCCTTACACAGCAAATGA+TGG	0.512248	3_3:-65008930	Msa0464810:CDS
GAATTTAGGACTACCTATGC+TGG	0.513702	3_3:-65009614	Msa0464810:CDS
GGATGAACAATGTATAATGA+TGG	0.515697	3_3:+65010067	None:intergenic
GGAACTCATGGGGAGCGGAA+TGG	0.524402	3_3:-65008399	Msa0464810:CDS
GTCCTCGGACAAAAGCTTGT+GGG	0.525849	3_3:-65009804	Msa0464810:CDS
AGGACTTTGTGGCATAGCTA+TGG	0.526821	3_3:-65008329	Msa0464810:CDS
TGTGACAATCAAGAAAATCA+AGG	0.527505	3_3:-65009026	Msa0464810:CDS
GAAAATCAAGGATGCAATGG+TGG	0.535112	3_3:-65009014	Msa0464810:CDS
GTCCTGTATAGGTAGTTGCT+GGG	0.537057	3_3:-65009135	Msa0464810:intron
TCCTCCGGCATCTATGGCTA+CGG	0.543132	3_3:+65008701	None:intergenic
GACTACCTATGCTGGATCAA+AGG	0.546319	3_3:-65009606	Msa0464810:CDS
CAAGAAAATCAAGGATGCAA+TGG	0.548537	3_3:-65009017	Msa0464810:CDS
AAACTGGAAATCAGATCCTC+CGG	0.552551	3_3:+65008686	None:intergenic
TCTGTTGATTGGAGAAAGAA+AGG	0.553167	3_3:-65009503	Msa0464810:CDS
GGCTACGGATACAGGTTGGT+TGG	0.553342	3_3:+65008716	None:intergenic
TTTAAGACATAATGGTTGCA+GGG	0.557691	3_3:+65008020	None:intergenic
AGGTGCTGTCACTGAGGTCA+AGG	0.558185	3_3:-65009483	Msa0464810:CDS
ATAATGGTTGCAGGGAATGA+GGG	0.560027	3_3:+65008028	None:intergenic
ATCCATCATTTGCTGTGTAA+GGG	0.560080	3_3:+65008928	None:intergenic
TGTCCACAACACTAACAAGA+TGG	0.563716	3_3:-65009687	Msa0464810:CDS
TGTGGTAAAGAGCTAAATCA+TGG	0.564153	3_3:-65008478	Msa0464810:CDS
CCAAGATAAAGATGTGTCCT+CGG	0.565632	3_3:-65009819	Msa0464810:CDS
GACCATCAATTGACACAGCT+GGG	0.568817	3_3:+65008787	None:intergenic
TTGTTCAAGTATGGAAGCAA+AGG	0.571573	3_3:+65010116	None:intergenic
CCAATAATTAGTTCCATCAA+CGG	0.573198	3_3:+65008429	None:intergenic
TGTGGGACTTATACGAGAGA+TGG	0.573304	3_3:-65009787	Msa0464810:CDS
TTGGATTGTGAGGAACTCAT+GGG	0.574375	3_3:-65008410	Msa0464810:CDS
AACTCATGGGGAGCGGAATG+GGG	0.577276	3_3:-65008397	Msa0464810:CDS
AAAGCATCTTCATCGTTAGC+CGG	0.584931	3_3:+65008753	None:intergenic
AAATTCATGGTTGGTCATGT+CGG	0.587621	3_3:+65009631	None:intergenic
GGAGCGGAATGGGGTGAACA+AGG	0.588520	3_3:-65008388	Msa0464810:CDS
ATTAACCTTTGATCCAGCAT+AGG	0.590030	3_3:+65009601	None:intergenic
ATCTTGGAAATCGAAGCTCT+CGG	0.596119	3_3:+65009835	None:intergenic
GGAGTATTTAGTGGCGATTG+TGG	0.600793	3_3:-65008496	Msa0464810:CDS
TACTTCAAGAACCAAAGCAA+TGG	0.600914	3_3:+65009859	None:intergenic
TGTGAGGAACTCATGGGGAG+CGG	0.601438	3_3:-65008404	Msa0464810:CDS
AAAAGGGAAAGAGCAGGGGA+TGG	0.601628	3_3:+65009977	None:intergenic
TGTCCTCGGACAAAAGCTTG+TGG	0.602219	3_3:-65009805	Msa0464810:CDS
ATGTTCCGAGGCACGCCACG+TGG	0.610728	3_3:-65009569	Msa0464810:CDS
GCGTGCCTCGGAACATTCTG+TGG	0.611727	3_3:+65009576	None:intergenic
AGGAATGTATCTAACAAGGA+AGG	0.612463	3_3:-65008349	Msa0464810:CDS
GAACTAATTATTGGATTGTG+AGG	0.613342	3_3:-65008420	Msa0464810:CDS
GGTAGTCCTAAATTCATGGT+TGG	0.613532	3_3:+65009622	None:intergenic
TGCTTCCAAACAAAGAAACA+TGG	0.616974	3_3:-65009912	Msa0464810:exon
AAAATCAAGGATGCAATGGT+GGG	0.617377	3_3:-65009013	Msa0464810:CDS
ATAAGCTCTTGTTCAGACAA+AGG	0.634536	3_3:+65009053	None:intergenic
AAGGTTACATCAGAATGCAA+AGG	0.641547	3_3:-65008369	Msa0464810:CDS
GCAAAGGAATGTATCTAACA+AGG	0.642601	3_3:-65008353	Msa0464810:CDS
CTATGGCTACGGATACAGGT+TGG	0.643007	3_3:+65008712	None:intergenic
GATGAAAAGGGAAAGAGCAG+GGG	0.645380	3_3:+65009973	None:intergenic
GGGACTTATACGAGAGATGG+AGG	0.652822	3_3:-65009784	Msa0464810:CDS
GTATCCGTAGCCATAGATGC+CGG	0.655334	3_3:-65008705	Msa0464810:CDS
AGGACCTTTATCATCTACCA+AGG	0.670444	3_3:-65008266	Msa0464810:CDS
AATCACCACAGAATGTTCCG+AGG	0.693791	3_3:-65009581	Msa0464810:CDS
TCCGTAGCCATAGATGCCGG+AGG	0.697215	3_3:-65008702	Msa0464810:CDS
GTCAAGGATCAAGGCCAATG+TGG	0.699707	3_3:-65009467	Msa0464810:intron
GAGATGGAGGAGTCACCACA+CGG	0.702988	3_3:-65009771	Msa0464810:CDS
TGGATTGTGAGGAACTCATG+GGG	0.709984	3_3:-65008409	Msa0464810:CDS
CAAGGTTCCGAGAAACCGTG+TGG	0.715039	3_3:+65009756	None:intergenic
AAAGAAAGGTGCTGTCACTG+AGG	0.722701	3_3:-65009489	Msa0464810:CDS
ATGAATGTTCCACTTCCACG+TGG	0.756623	3_3:+65009554	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAAAATATATGATCT+AGG	+	chr3_3:65008902-65008921	None:intergenic	10.0%
!!!	TTTTTACTTTTTTATTTTGA+AGG	+	chr3_3:65009512-65009531	None:intergenic	10.0%
!!	AAAAAAAAAATATATGATCT+AGG	+	chr3_3:65008902-65008921	None:intergenic	10.0%
!!!	TTTTTACTTTTTTATTTTGA+AGG	+	chr3_3:65009512-65009531	None:intergenic	10.0%
!!!	ATGAATAATTTTTGAATCAT+AGG	+	chr3_3:65009287-65009306	None:intergenic	15.0%
!!	TAAAAAAGTAAAAAAAGAGA+TGG	-	chr3_3:65009518-65009537	Msa0464810:CDS	15.0%
!!!	TCAAATATTTAAAGTTCATA+AGG	+	chr3_3:65009955-65009974	None:intergenic	15.0%
!!!	ATGAATAATTTTTGAATCAT+AGG	+	chr3_3:65009287-65009306	None:intergenic	15.0%
!!	TAAAAAAGTAAAAAAAGAGA+TGG	-	chr3_3:65009518-65009537	Msa0464810:CDS	15.0%
!!!	TCAAATATTTAAAGTTCATA+AGG	+	chr3_3:65009955-65009974	None:intergenic	15.0%
!!	AAACTCAATAAAAAAATGTG+AGG	+	chr3_3:65008088-65008107	None:intergenic	20.0%
!!	TTTCGAATGTATTAATTGAA+TGG	+	chr3_3:65008168-65008187	None:intergenic	20.0%
!!	TAAACCAAATAAAGACAAAT+AGG	-	chr3_3:65009027-65009046	Msa0464810:CDS	20.0%
!!!	ATGAATGTTTTAAGACATAA+TGG	+	chr3_3:65010100-65010119	None:intergenic	20.0%
!!	AACATTCATTCAAATATCAA+TGG	-	chr3_3:65010111-65010130	Msa0464810:five_prime_UTR	20.0%
!!	AAACTCAATAAAAAAATGTG+AGG	+	chr3_3:65008088-65008107	None:intergenic	20.0%
!!	TTTCGAATGTATTAATTGAA+TGG	+	chr3_3:65008168-65008187	None:intergenic	20.0%
!!	TAAACCAAATAAAGACAAAT+AGG	-	chr3_3:65009027-65009046	Msa0464810:CDS	20.0%
!!!	ATGAATGTTTTAAGACATAA+TGG	+	chr3_3:65010100-65010119	None:intergenic	20.0%
!!	AACATTCATTCAAATATCAA+TGG	-	chr3_3:65010111-65010130	Msa0464810:five_prime_UTR	20.0%
!!	AATGATGGAGATGATAAAAT+AGG	+	chr3_3:65008030-65008049	None:intergenic	25.0%
!	TGTATTAATTGAATGGAAGT+TGG	+	chr3_3:65008161-65008180	None:intergenic	25.0%
!	TTGTTAAGTTTCAACTTGTA+AGG	+	chr3_3:65008453-65008472	None:intergenic	25.0%
!!!	ACTTTTTTAACTTACCACAT+TGG	+	chr3_3:65008659-65008678	None:intergenic	25.0%
!	TATAATTGACATGCAACTTT+AGG	-	chr3_3:65009259-65009278	Msa0464810:intron	25.0%
!	TCAAAAATTATTCATGTTGC+AGG	-	chr3_3:65009292-65009311	Msa0464810:intron	25.0%
!!	ATTTTGTATACACCTATTAC+AGG	-	chr3_3:65009591-65009610	Msa0464810:CDS	25.0%
!!	TTTTGTATACACCTATTACA+GGG	-	chr3_3:65009592-65009611	Msa0464810:CDS	25.0%
!!	AATGATGGAGATGATAAAAT+AGG	+	chr3_3:65008030-65008049	None:intergenic	25.0%
!	TGTATTAATTGAATGGAAGT+TGG	+	chr3_3:65008161-65008180	None:intergenic	25.0%
!	TTGTTAAGTTTCAACTTGTA+AGG	+	chr3_3:65008453-65008472	None:intergenic	25.0%
!!!	ACTTTTTTAACTTACCACAT+TGG	+	chr3_3:65008659-65008678	None:intergenic	25.0%
!	TATAATTGACATGCAACTTT+AGG	-	chr3_3:65009259-65009278	Msa0464810:intron	25.0%
!	TCAAAAATTATTCATGTTGC+AGG	-	chr3_3:65009292-65009311	Msa0464810:intron	25.0%
!!	ATTTTGTATACACCTATTAC+AGG	-	chr3_3:65009591-65009610	Msa0464810:CDS	25.0%
!!	TTTTGTATACACCTATTACA+GGG	-	chr3_3:65009592-65009611	Msa0464810:CDS	25.0%
!	ATGTTTGTGTTGTTCAAGTA+TGG	+	chr3_3:65008005-65008024	None:intergenic	30.0%
	GGATGAACAATGTATAATGA+TGG	+	chr3_3:65008045-65008064	None:intergenic	30.0%
	AAAAAATGTGAGGAGGTTAA+AGG	+	chr3_3:65008078-65008097	None:intergenic	30.0%
	CTCAATAAAAAAATGTGAGG+AGG	+	chr3_3:65008085-65008104	None:intergenic	30.0%
	TTATATAGAGGATTGGAGAA+AGG	+	chr3_3:65008111-65008130	None:intergenic	30.0%
	AGTTTCCATGTTTCTTTGTT+TGG	+	chr3_3:65008205-65008224	None:intergenic	30.0%
	TTGTGATTAATGTCCTGTAT+AGG	-	chr3_3:65008963-65008982	Msa0464810:CDS	30.0%
	CTAGCCTATTTGTCTTTATT+TGG	+	chr3_3:65009034-65009053	None:intergenic	30.0%
	TGTGACAATCAAGAAAATCA+AGG	-	chr3_3:65009083-65009102	Msa0464810:CDS	30.0%
	TTTGAGTACATCAAGCAAAA+AGG	-	chr3_3:65009134-65009153	Msa0464810:intron	30.0%
!	TTTTTACCTCTGAGTAAAAC+TGG	+	chr3_3:65009442-65009461	None:intergenic	30.0%
	CCAATAATTAGTTCCATCAA+CGG	+	chr3_3:65009683-65009702	None:intergenic	30.0%
	GAACTAATTATTGGATTGTG+AGG	-	chr3_3:65009689-65009708	Msa0464810:CDS	30.0%
!!	TTTTGGAGGAGTTTTTAACT+GGG	+	chr3_3:65009816-65009835	None:intergenic	30.0%
!!	TTTTTGGAGGAGTTTTTAAC+TGG	+	chr3_3:65009817-65009836	None:intergenic	30.0%
!	TTTGATGACAACGTATGATT+TGG	-	chr3_3:65009983-65010002	Msa0464810:five_prime_UTR	30.0%
!	TTTAAGACATAATGGTTGCA+GGG	+	chr3_3:65010092-65010111	None:intergenic	30.0%
!!!	TTTTAAGACATAATGGTTGC+AGG	+	chr3_3:65010093-65010112	None:intergenic	30.0%
!	ATGTTTGTGTTGTTCAAGTA+TGG	+	chr3_3:65008005-65008024	None:intergenic	30.0%
	GGATGAACAATGTATAATGA+TGG	+	chr3_3:65008045-65008064	None:intergenic	30.0%
	AAAAAATGTGAGGAGGTTAA+AGG	+	chr3_3:65008078-65008097	None:intergenic	30.0%
	CTCAATAAAAAAATGTGAGG+AGG	+	chr3_3:65008085-65008104	None:intergenic	30.0%
	TTATATAGAGGATTGGAGAA+AGG	+	chr3_3:65008111-65008130	None:intergenic	30.0%
	AGTTTCCATGTTTCTTTGTT+TGG	+	chr3_3:65008205-65008224	None:intergenic	30.0%
	TTGTGATTAATGTCCTGTAT+AGG	-	chr3_3:65008963-65008982	Msa0464810:CDS	30.0%
	CTAGCCTATTTGTCTTTATT+TGG	+	chr3_3:65009034-65009053	None:intergenic	30.0%
	TGTGACAATCAAGAAAATCA+AGG	-	chr3_3:65009083-65009102	Msa0464810:CDS	30.0%
	TTTGAGTACATCAAGCAAAA+AGG	-	chr3_3:65009134-65009153	Msa0464810:intron	30.0%
!	TTTTTACCTCTGAGTAAAAC+TGG	+	chr3_3:65009442-65009461	None:intergenic	30.0%
	CCAATAATTAGTTCCATCAA+CGG	+	chr3_3:65009683-65009702	None:intergenic	30.0%
	GAACTAATTATTGGATTGTG+AGG	-	chr3_3:65009689-65009708	Msa0464810:CDS	30.0%
!!	TTTTGGAGGAGTTTTTAACT+GGG	+	chr3_3:65009816-65009835	None:intergenic	30.0%
!!	TTTTTGGAGGAGTTTTTAAC+TGG	+	chr3_3:65009817-65009836	None:intergenic	30.0%
!	TTTGATGACAACGTATGATT+TGG	-	chr3_3:65009983-65010002	Msa0464810:five_prime_UTR	30.0%
!	TTTAAGACATAATGGTTGCA+GGG	+	chr3_3:65010092-65010111	None:intergenic	30.0%
!!!	TTTTAAGACATAATGGTTGC+AGG	+	chr3_3:65010093-65010112	None:intergenic	30.0%
	TTGTTCAAGTATGGAAGCAA+AGG	+	chr3_3:65007996-65008015	None:intergenic	35.0%
	GAGGTTAAAGGTTTCTTAAG+GGG	+	chr3_3:65008066-65008085	None:intergenic	35.0%
	GGAGGTTAAAGGTTTCTTAA+GGG	+	chr3_3:65008067-65008086	None:intergenic	35.0%
	AGGAGGTTAAAGGTTTCTTA+AGG	+	chr3_3:65008068-65008087	None:intergenic	35.0%
	GGATGGTTTATATAGAGGAT+TGG	+	chr3_3:65008118-65008137	None:intergenic	35.0%
	GAATGGAAGTTGGATGAAAA+GGG	+	chr3_3:65008151-65008170	None:intergenic	35.0%
	TGAATGGAAGTTGGATGAAA+AGG	+	chr3_3:65008152-65008171	None:intergenic	35.0%
	TGCTTCCAAACAAAGAAACA+TGG	-	chr3_3:65008197-65008216	Msa0464810:three_prime_UTR	35.0%
	TACTTCAAGAACCAAAGCAA+TGG	+	chr3_3:65008253-65008272	None:intergenic	35.0%
	TATCCATCTTGTTAGTGTTG+TGG	+	chr3_3:65008428-65008447	None:intergenic	35.0%
!	AAATTCATGGTTGGTCATGT+CGG	+	chr3_3:65008481-65008500	None:intergenic	35.0%
	ACATGACCAACCATGAATTT+AGG	-	chr3_3:65008481-65008500	Msa0464810:CDS	35.0%
	CATAGGTAGTCCTAAATTCA+TGG	+	chr3_3:65008494-65008513	None:intergenic	35.0%
	ATTAACCTTTGATCCAGCAT+AGG	+	chr3_3:65008511-65008530	None:intergenic	35.0%
!	TCTGTTGATTGGAGAAAGAA+AGG	-	chr3_3:65008606-65008625	Msa0464810:intron	35.0%
	GACACCATCTAATCAAAGAT+CGG	-	chr3_3:65008729-65008748	Msa0464810:CDS	35.0%
	ATAAGCTCTTGTTCAGACAA+AGG	+	chr3_3:65009059-65009078	None:intergenic	35.0%
	CAAGAAAATCAAGGATGCAA+TGG	-	chr3_3:65009092-65009111	Msa0464810:CDS	35.0%
	AAAATCAAGGATGCAATGGT+GGG	-	chr3_3:65009096-65009115	Msa0464810:CDS	35.0%
!!	ATCCATCATTTGCTGTGTAA+GGG	+	chr3_3:65009184-65009203	None:intergenic	35.0%
!	TGATTTCCAGTTTTACTCAG+AGG	-	chr3_3:65009433-65009452	Msa0464810:intron	35.0%
	ATTCTCTTGTGTCAATAGAG+AGG	+	chr3_3:65009476-65009495	None:intergenic	35.0%
	CACTAAATACTCCCTGTAAT+AGG	+	chr3_3:65009606-65009625	None:intergenic	35.0%
!!	CTATTACAGGGAGTATTTAG+TGG	-	chr3_3:65009604-65009623	Msa0464810:CDS	35.0%
	TGTGGTAAAGAGCTAAATCA+TGG	-	chr3_3:65009631-65009650	Msa0464810:CDS	35.0%
	AATCATGGTGTAGCAATAGT+AGG	-	chr3_3:65009646-65009665	Msa0464810:CDS	35.0%
!	CCGTTGATGGAACTAATTAT+TGG	-	chr3_3:65009680-65009699	Msa0464810:CDS	35.0%
	AAGGTTACATCAGAATGCAA+AGG	-	chr3_3:65009740-65009759	Msa0464810:CDS	35.0%
	GCAAAGGAATGTATCTAACA+AGG	-	chr3_3:65009756-65009775	Msa0464810:CDS	35.0%
	AGGAATGTATCTAACAAGGA+AGG	-	chr3_3:65009760-65009779	Msa0464810:CDS	35.0%
	TCATCCTTGGTAGATGATAA+AGG	+	chr3_3:65009850-65009869	None:intergenic	35.0%
	AAACATTCAAAGCTCATCCT+TGG	+	chr3_3:65009863-65009882	None:intergenic	35.0%
	TTGTTCAAGTATGGAAGCAA+AGG	+	chr3_3:65007996-65008015	None:intergenic	35.0%
	GAGGTTAAAGGTTTCTTAAG+GGG	+	chr3_3:65008066-65008085	None:intergenic	35.0%
	GGAGGTTAAAGGTTTCTTAA+GGG	+	chr3_3:65008067-65008086	None:intergenic	35.0%
	AGGAGGTTAAAGGTTTCTTA+AGG	+	chr3_3:65008068-65008087	None:intergenic	35.0%
	GGATGGTTTATATAGAGGAT+TGG	+	chr3_3:65008118-65008137	None:intergenic	35.0%
	GAATGGAAGTTGGATGAAAA+GGG	+	chr3_3:65008151-65008170	None:intergenic	35.0%
	TGAATGGAAGTTGGATGAAA+AGG	+	chr3_3:65008152-65008171	None:intergenic	35.0%
	TGCTTCCAAACAAAGAAACA+TGG	-	chr3_3:65008197-65008216	Msa0464810:three_prime_UTR	35.0%
	TACTTCAAGAACCAAAGCAA+TGG	+	chr3_3:65008253-65008272	None:intergenic	35.0%
	TATCCATCTTGTTAGTGTTG+TGG	+	chr3_3:65008428-65008447	None:intergenic	35.0%
!	AAATTCATGGTTGGTCATGT+CGG	+	chr3_3:65008481-65008500	None:intergenic	35.0%
	ACATGACCAACCATGAATTT+AGG	-	chr3_3:65008481-65008500	Msa0464810:CDS	35.0%
	CATAGGTAGTCCTAAATTCA+TGG	+	chr3_3:65008494-65008513	None:intergenic	35.0%
	ATTAACCTTTGATCCAGCAT+AGG	+	chr3_3:65008511-65008530	None:intergenic	35.0%
!	TCTGTTGATTGGAGAAAGAA+AGG	-	chr3_3:65008606-65008625	Msa0464810:intron	35.0%
	GACACCATCTAATCAAAGAT+CGG	-	chr3_3:65008729-65008748	Msa0464810:CDS	35.0%
	ATAAGCTCTTGTTCAGACAA+AGG	+	chr3_3:65009059-65009078	None:intergenic	35.0%
	CAAGAAAATCAAGGATGCAA+TGG	-	chr3_3:65009092-65009111	Msa0464810:CDS	35.0%
	AAAATCAAGGATGCAATGGT+GGG	-	chr3_3:65009096-65009115	Msa0464810:CDS	35.0%
!!	ATCCATCATTTGCTGTGTAA+GGG	+	chr3_3:65009184-65009203	None:intergenic	35.0%
!	TGATTTCCAGTTTTACTCAG+AGG	-	chr3_3:65009433-65009452	Msa0464810:intron	35.0%
	ATTCTCTTGTGTCAATAGAG+AGG	+	chr3_3:65009476-65009495	None:intergenic	35.0%
	CACTAAATACTCCCTGTAAT+AGG	+	chr3_3:65009606-65009625	None:intergenic	35.0%
!!	CTATTACAGGGAGTATTTAG+TGG	-	chr3_3:65009604-65009623	Msa0464810:CDS	35.0%
	TGTGGTAAAGAGCTAAATCA+TGG	-	chr3_3:65009631-65009650	Msa0464810:CDS	35.0%
	AATCATGGTGTAGCAATAGT+AGG	-	chr3_3:65009646-65009665	Msa0464810:CDS	35.0%
!	CCGTTGATGGAACTAATTAT+TGG	-	chr3_3:65009680-65009699	Msa0464810:CDS	35.0%
	AAGGTTACATCAGAATGCAA+AGG	-	chr3_3:65009740-65009759	Msa0464810:CDS	35.0%
	GCAAAGGAATGTATCTAACA+AGG	-	chr3_3:65009756-65009775	Msa0464810:CDS	35.0%
	AGGAATGTATCTAACAAGGA+AGG	-	chr3_3:65009760-65009779	Msa0464810:CDS	35.0%
	TCATCCTTGGTAGATGATAA+AGG	+	chr3_3:65009850-65009869	None:intergenic	35.0%
	AAACATTCAAAGCTCATCCT+TGG	+	chr3_3:65009863-65009882	None:intergenic	35.0%
	GATTGTTCTGTCCATTGCTT+TGG	-	chr3_3:65008239-65008258	Msa0464810:exon	40.0%
	ATCTTGGAAATCGAAGCTCT+CGG	+	chr3_3:65008277-65008296	None:intergenic	40.0%
	CCAAGATAAAGATGTGTCCT+CGG	-	chr3_3:65008290-65008309	Msa0464810:CDS	40.0%
	TGTCCACAACACTAACAAGA+TGG	-	chr3_3:65008422-65008441	Msa0464810:CDS	40.0%
	GGTAGTCCTAAATTCATGGT+TGG	+	chr3_3:65008490-65008509	None:intergenic	40.0%
!	GAATTTAGGACTACCTATGC+TGG	-	chr3_3:65008495-65008514	Msa0464810:CDS	40.0%
	TTTCTCCAATCAACAGAAGC+AGG	+	chr3_3:65008603-65008622	None:intergenic	40.0%
	ACTGTGTGAATGCAAAGTGA+TGG	+	chr3_3:65008700-65008719	None:intergenic	40.0%
	TCGTCCGATCTTTGATTAGA+TGG	+	chr3_3:65008736-65008755	None:intergenic	40.0%
	TCAACTGTTGTAGCTGTTGA+AGG	-	chr3_3:65009002-65009021	Msa0464810:CDS	40.0%
	GAAAATCAAGGATGCAATGG+TGG	-	chr3_3:65009095-65009114	Msa0464810:CDS	40.0%
	GAGTACATCAAGCAAAAAGG+TGG	-	chr3_3:65009137-65009156	Msa0464810:intron	40.0%
	TACCCTTACACAGCAAATGA+TGG	-	chr3_3:65009179-65009198	Msa0464810:intron	40.0%
!!	GATCCATCATTTGCTGTGTA+AGG	+	chr3_3:65009185-65009204	None:intergenic	40.0%
!	AAAGCATCTTCATCGTTAGC+CGG	+	chr3_3:65009359-65009378	None:intergenic	40.0%
	AAACTGGAAATCAGATCCTC+CGG	+	chr3_3:65009426-65009445	None:intergenic	40.0%
	GGTGTAGCAATAGTAGGATA+TGG	-	chr3_3:65009652-65009671	Msa0464810:CDS	40.0%
	ATTGGATTGTGAGGAACTCA+TGG	-	chr3_3:65009698-65009717	Msa0464810:CDS	40.0%
	TTGGATTGTGAGGAACTCAT+GGG	-	chr3_3:65009699-65009718	Msa0464810:CDS	40.0%
	TCTAACAAGGAAGGACTTTG+TGG	-	chr3_3:65009769-65009788	Msa0464810:CDS	40.0%
!	TAAAGGTCCTGCTGGATTTT+TGG	+	chr3_3:65009833-65009852	None:intergenic	40.0%
	AGGACCTTTATCATCTACCA+AGG	-	chr3_3:65009843-65009862	Msa0464810:CDS	40.0%
	ATAATGGTTGCAGGGAATGA+GGG	+	chr3_3:65010084-65010103	None:intergenic	40.0%
	GATTGTTCTGTCCATTGCTT+TGG	-	chr3_3:65008239-65008258	Msa0464810:exon	40.0%
	ATCTTGGAAATCGAAGCTCT+CGG	+	chr3_3:65008277-65008296	None:intergenic	40.0%
	CCAAGATAAAGATGTGTCCT+CGG	-	chr3_3:65008290-65008309	Msa0464810:CDS	40.0%
	TGTCCACAACACTAACAAGA+TGG	-	chr3_3:65008422-65008441	Msa0464810:CDS	40.0%
	GGTAGTCCTAAATTCATGGT+TGG	+	chr3_3:65008490-65008509	None:intergenic	40.0%
!	GAATTTAGGACTACCTATGC+TGG	-	chr3_3:65008495-65008514	Msa0464810:CDS	40.0%
	TTTCTCCAATCAACAGAAGC+AGG	+	chr3_3:65008603-65008622	None:intergenic	40.0%
	ACTGTGTGAATGCAAAGTGA+TGG	+	chr3_3:65008700-65008719	None:intergenic	40.0%
	TCGTCCGATCTTTGATTAGA+TGG	+	chr3_3:65008736-65008755	None:intergenic	40.0%
	TCAACTGTTGTAGCTGTTGA+AGG	-	chr3_3:65009002-65009021	Msa0464810:CDS	40.0%
	GAAAATCAAGGATGCAATGG+TGG	-	chr3_3:65009095-65009114	Msa0464810:CDS	40.0%
	GAGTACATCAAGCAAAAAGG+TGG	-	chr3_3:65009137-65009156	Msa0464810:intron	40.0%
	TACCCTTACACAGCAAATGA+TGG	-	chr3_3:65009179-65009198	Msa0464810:intron	40.0%
!!	GATCCATCATTTGCTGTGTA+AGG	+	chr3_3:65009185-65009204	None:intergenic	40.0%
!	AAAGCATCTTCATCGTTAGC+CGG	+	chr3_3:65009359-65009378	None:intergenic	40.0%
	AAACTGGAAATCAGATCCTC+CGG	+	chr3_3:65009426-65009445	None:intergenic	40.0%
	GGTGTAGCAATAGTAGGATA+TGG	-	chr3_3:65009652-65009671	Msa0464810:CDS	40.0%
	ATTGGATTGTGAGGAACTCA+TGG	-	chr3_3:65009698-65009717	Msa0464810:CDS	40.0%
	TTGGATTGTGAGGAACTCAT+GGG	-	chr3_3:65009699-65009718	Msa0464810:CDS	40.0%
	TCTAACAAGGAAGGACTTTG+TGG	-	chr3_3:65009769-65009788	Msa0464810:CDS	40.0%
!	TAAAGGTCCTGCTGGATTTT+TGG	+	chr3_3:65009833-65009852	None:intergenic	40.0%
	AGGACCTTTATCATCTACCA+AGG	-	chr3_3:65009843-65009862	Msa0464810:CDS	40.0%
	ATAATGGTTGCAGGGAATGA+GGG	+	chr3_3:65010084-65010103	None:intergenic	40.0%
	GCAGGGGATGGTTTATATAG+AGG	+	chr3_3:65008123-65008142	None:intergenic	45.0%
	GATGAAAAGGGAAAGAGCAG+GGG	+	chr3_3:65008139-65008158	None:intergenic	45.0%
	GGATGAAAAGGGAAAGAGCA+GGG	+	chr3_3:65008140-65008159	None:intergenic	45.0%
	TGGATGAAAAGGGAAAGAGC+AGG	+	chr3_3:65008141-65008160	None:intergenic	45.0%
	CCGAGGACACATCTTTATCT+TGG	+	chr3_3:65008293-65008312	None:intergenic	45.0%
	TGTGGGACTTATACGAGAGA+TGG	-	chr3_3:65008322-65008341	Msa0464810:CDS	45.0%
!!	AGCGCTTTTGTTTCTCGTCA+AGG	+	chr3_3:65008374-65008393	None:intergenic	45.0%
	GACTACCTATGCTGGATCAA+AGG	-	chr3_3:65008503-65008522	Msa0464810:intron	45.0%
	AATCACCACAGAATGTTCCG+AGG	-	chr3_3:65008528-65008547	Msa0464810:intron	45.0%
	ATGAATGTTCCACTTCCACG+TGG	+	chr3_3:65008558-65008577	None:intergenic	45.0%
	ATCAACAGAAGCAGGAGCTT+TGG	+	chr3_3:65008595-65008614	None:intergenic	45.0%
	AAGCTCCTGCTTCTGTTGAT+TGG	-	chr3_3:65008595-65008614	Msa0464810:intron	45.0%
!!	AAAGAAAGGTGCTGTCACTG+AGG	-	chr3_3:65008620-65008639	Msa0464810:intron	45.0%
	TGTCCTGTATAGGTAGTTGC+TGG	-	chr3_3:65008973-65008992	Msa0464810:CDS	45.0%
	GTCCTGTATAGGTAGTTGCT+GGG	-	chr3_3:65008974-65008993	Msa0464810:CDS	45.0%
	GACCATCAATTGACACAGCT+GGG	+	chr3_3:65009325-65009344	None:intergenic	45.0%
	TGACCATCAATTGACACAGC+TGG	+	chr3_3:65009326-65009345	None:intergenic	45.0%
	TGATGGTCATGAGACTGTTC+CGG	-	chr3_3:65009337-65009356	Msa0464810:intron	45.0%
!	GGAGTATTTAGTGGCGATTG+TGG	-	chr3_3:65009613-65009632	Msa0464810:CDS	45.0%
	GGATATGGAACAACCGTTGA+TGG	-	chr3_3:65009667-65009686	Msa0464810:CDS	45.0%
	TGGATTGTGAGGAACTCATG+GGG	-	chr3_3:65009700-65009719	Msa0464810:CDS	45.0%
	AGGACTTTGTGGCATAGCTA+TGG	-	chr3_3:65009780-65009799	Msa0464810:CDS	45.0%
!!	GGAGGAGTTTTTAACTGGGT+AGG	+	chr3_3:65009812-65009831	None:intergenic	45.0%
	AACTCCTCCAAAAATCCAGC+AGG	-	chr3_3:65009823-65009842	Msa0464810:CDS	45.0%
	GTAGATGATAAAGGTCCTGC+TGG	+	chr3_3:65009841-65009860	None:intergenic	45.0%
	CATAATGGTTGCAGGGAATG+AGG	+	chr3_3:65010085-65010104	None:intergenic	45.0%
	GCAGGGGATGGTTTATATAG+AGG	+	chr3_3:65008123-65008142	None:intergenic	45.0%
	GATGAAAAGGGAAAGAGCAG+GGG	+	chr3_3:65008139-65008158	None:intergenic	45.0%
	GGATGAAAAGGGAAAGAGCA+GGG	+	chr3_3:65008140-65008159	None:intergenic	45.0%
	TGGATGAAAAGGGAAAGAGC+AGG	+	chr3_3:65008141-65008160	None:intergenic	45.0%
	CCGAGGACACATCTTTATCT+TGG	+	chr3_3:65008293-65008312	None:intergenic	45.0%
	TGTGGGACTTATACGAGAGA+TGG	-	chr3_3:65008322-65008341	Msa0464810:CDS	45.0%
!!	AGCGCTTTTGTTTCTCGTCA+AGG	+	chr3_3:65008374-65008393	None:intergenic	45.0%
	GACTACCTATGCTGGATCAA+AGG	-	chr3_3:65008503-65008522	Msa0464810:intron	45.0%
	AATCACCACAGAATGTTCCG+AGG	-	chr3_3:65008528-65008547	Msa0464810:intron	45.0%
	ATGAATGTTCCACTTCCACG+TGG	+	chr3_3:65008558-65008577	None:intergenic	45.0%
	ATCAACAGAAGCAGGAGCTT+TGG	+	chr3_3:65008595-65008614	None:intergenic	45.0%
	AAGCTCCTGCTTCTGTTGAT+TGG	-	chr3_3:65008595-65008614	Msa0464810:intron	45.0%
!!	AAAGAAAGGTGCTGTCACTG+AGG	-	chr3_3:65008620-65008639	Msa0464810:intron	45.0%
	TGTCCTGTATAGGTAGTTGC+TGG	-	chr3_3:65008973-65008992	Msa0464810:CDS	45.0%
	GTCCTGTATAGGTAGTTGCT+GGG	-	chr3_3:65008974-65008993	Msa0464810:CDS	45.0%
	GACCATCAATTGACACAGCT+GGG	+	chr3_3:65009325-65009344	None:intergenic	45.0%
	TGACCATCAATTGACACAGC+TGG	+	chr3_3:65009326-65009345	None:intergenic	45.0%
	TGATGGTCATGAGACTGTTC+CGG	-	chr3_3:65009337-65009356	Msa0464810:intron	45.0%
!	GGAGTATTTAGTGGCGATTG+TGG	-	chr3_3:65009613-65009632	Msa0464810:CDS	45.0%
	GGATATGGAACAACCGTTGA+TGG	-	chr3_3:65009667-65009686	Msa0464810:CDS	45.0%
	TGGATTGTGAGGAACTCATG+GGG	-	chr3_3:65009700-65009719	Msa0464810:CDS	45.0%
	AGGACTTTGTGGCATAGCTA+TGG	-	chr3_3:65009780-65009799	Msa0464810:CDS	45.0%
!!	GGAGGAGTTTTTAACTGGGT+AGG	+	chr3_3:65009812-65009831	None:intergenic	45.0%
	AACTCCTCCAAAAATCCAGC+AGG	-	chr3_3:65009823-65009842	Msa0464810:CDS	45.0%
	GTAGATGATAAAGGTCCTGC+TGG	+	chr3_3:65009841-65009860	None:intergenic	45.0%
	CATAATGGTTGCAGGGAATG+AGG	+	chr3_3:65010085-65010104	None:intergenic	45.0%
	AAAAGGGAAAGAGCAGGGGA+TGG	+	chr3_3:65008135-65008154	None:intergenic	50.0%
	TGTCCTCGGACAAAAGCTTG+TGG	-	chr3_3:65008304-65008323	Msa0464810:CDS	50.0%
	GTCCTCGGACAAAAGCTTGT+GGG	-	chr3_3:65008305-65008324	Msa0464810:CDS	50.0%
	GGGACTTATACGAGAGATGG+AGG	-	chr3_3:65008325-65008344	Msa0464810:CDS	50.0%
	GTCACTGAGGTCAAGGATCA+AGG	-	chr3_3:65008633-65008652	Msa0464810:intron	50.0%
	GTCAAGGATCAAGGCCAATG+TGG	-	chr3_3:65008642-65008661	Msa0464810:intron	50.0%
	AGCCCAGCAACTACCTATAC+AGG	+	chr3_3:65008979-65008998	None:intergenic	50.0%
!!	AGGATGCAATGGTGGGCTAA+TGG	-	chr3_3:65009103-65009122	Msa0464810:CDS	50.0%
	GTCCCAGCTGTGTCAATTGA+TGG	-	chr3_3:65009320-65009339	Msa0464810:intron	50.0%
	CTATGGCTACGGATACAGGT+TGG	+	chr3_3:65009400-65009419	None:intergenic	50.0%
	GCATCTATGGCTACGGATAC+AGG	+	chr3_3:65009404-65009423	None:intergenic	50.0%
	GTATCCGTAGCCATAGATGC+CGG	-	chr3_3:65009404-65009423	Msa0464810:intron	50.0%
	ATCAGATCCTCCGGCATCTA+TGG	+	chr3_3:65009417-65009436	None:intergenic	50.0%
!!	AGGTCCTGCTGGATTTTTGG+AGG	+	chr3_3:65009830-65009849	None:intergenic	50.0%
	AAAAGGGAAAGAGCAGGGGA+TGG	+	chr3_3:65008135-65008154	None:intergenic	50.0%
	TGTCCTCGGACAAAAGCTTG+TGG	-	chr3_3:65008304-65008323	Msa0464810:CDS	50.0%
	GTCCTCGGACAAAAGCTTGT+GGG	-	chr3_3:65008305-65008324	Msa0464810:CDS	50.0%
	GGGACTTATACGAGAGATGG+AGG	-	chr3_3:65008325-65008344	Msa0464810:CDS	50.0%
	GTCACTGAGGTCAAGGATCA+AGG	-	chr3_3:65008633-65008652	Msa0464810:intron	50.0%
	GTCAAGGATCAAGGCCAATG+TGG	-	chr3_3:65008642-65008661	Msa0464810:intron	50.0%
	AGCCCAGCAACTACCTATAC+AGG	+	chr3_3:65008979-65008998	None:intergenic	50.0%
!!	AGGATGCAATGGTGGGCTAA+TGG	-	chr3_3:65009103-65009122	Msa0464810:CDS	50.0%
	GTCCCAGCTGTGTCAATTGA+TGG	-	chr3_3:65009320-65009339	Msa0464810:intron	50.0%
	CTATGGCTACGGATACAGGT+TGG	+	chr3_3:65009400-65009419	None:intergenic	50.0%
	GCATCTATGGCTACGGATAC+AGG	+	chr3_3:65009404-65009423	None:intergenic	50.0%
	GTATCCGTAGCCATAGATGC+CGG	-	chr3_3:65009404-65009423	Msa0464810:intron	50.0%
	ATCAGATCCTCCGGCATCTA+TGG	+	chr3_3:65009417-65009436	None:intergenic	50.0%
!!	AGGTCCTGCTGGATTTTTGG+AGG	+	chr3_3:65009830-65009849	None:intergenic	50.0%
!	GTCCCACAAGCTTTTGTCCG+AGG	+	chr3_3:65008310-65008329	None:intergenic	55.0%
	GAGATGGAGGAGTCACCACA+CGG	-	chr3_3:65008338-65008357	Msa0464810:CDS	55.0%
	GGAGTCACCACACGGTTTCT+CGG	-	chr3_3:65008346-65008365	Msa0464810:CDS	55.0%
	CAAGGTTCCGAGAAACCGTG+TGG	+	chr3_3:65008356-65008375	None:intergenic	55.0%
!	AGGTGCTGTCACTGAGGTCA+AGG	-	chr3_3:65008626-65008645	Msa0464810:intron	55.0%
!!	GGCTACGGATACAGGTTGGT+TGG	+	chr3_3:65009396-65009415	None:intergenic	55.0%
	TCCTCCGGCATCTATGGCTA+CGG	+	chr3_3:65009411-65009430	None:intergenic	55.0%
	TGTGAGGAACTCATGGGGAG+CGG	-	chr3_3:65009705-65009724	Msa0464810:CDS	55.0%
	GAACTCATGGGGAGCGGAAT+GGG	-	chr3_3:65009711-65009730	Msa0464810:CDS	55.0%
	AACTCATGGGGAGCGGAATG+GGG	-	chr3_3:65009712-65009731	Msa0464810:CDS	55.0%
!	GTCCCACAAGCTTTTGTCCG+AGG	+	chr3_3:65008310-65008329	None:intergenic	55.0%
	GAGATGGAGGAGTCACCACA+CGG	-	chr3_3:65008338-65008357	Msa0464810:CDS	55.0%
	GGAGTCACCACACGGTTTCT+CGG	-	chr3_3:65008346-65008365	Msa0464810:CDS	55.0%
	CAAGGTTCCGAGAAACCGTG+TGG	+	chr3_3:65008356-65008375	None:intergenic	55.0%
!	AGGTGCTGTCACTGAGGTCA+AGG	-	chr3_3:65008626-65008645	Msa0464810:intron	55.0%
!!	GGCTACGGATACAGGTTGGT+TGG	+	chr3_3:65009396-65009415	None:intergenic	55.0%
	TCCTCCGGCATCTATGGCTA+CGG	+	chr3_3:65009411-65009430	None:intergenic	55.0%
	TGTGAGGAACTCATGGGGAG+CGG	-	chr3_3:65009705-65009724	Msa0464810:CDS	55.0%
	GAACTCATGGGGAGCGGAAT+GGG	-	chr3_3:65009711-65009730	Msa0464810:CDS	55.0%
	AACTCATGGGGAGCGGAATG+GGG	-	chr3_3:65009712-65009731	Msa0464810:CDS	55.0%
	GCGTGCCTCGGAACATTCTG+TGG	+	chr3_3:65008536-65008555	None:intergenic	60.0%
	TCCGTAGCCATAGATGCCGG+AGG	-	chr3_3:65009407-65009426	Msa0464810:intron	60.0%
	GGAACTCATGGGGAGCGGAA+TGG	-	chr3_3:65009710-65009729	Msa0464810:CDS	60.0%
	GGAGCGGAATGGGGTGAACA+AGG	-	chr3_3:65009721-65009740	Msa0464810:CDS	60.0%
	GCGTGCCTCGGAACATTCTG+TGG	+	chr3_3:65008536-65008555	None:intergenic	60.0%
	TCCGTAGCCATAGATGCCGG+AGG	-	chr3_3:65009407-65009426	Msa0464810:intron	60.0%
	GGAACTCATGGGGAGCGGAA+TGG	-	chr3_3:65009710-65009729	Msa0464810:CDS	60.0%
	GGAGCGGAATGGGGTGAACA+AGG	-	chr3_3:65009721-65009740	Msa0464810:CDS	60.0%
	ATGTTCCGAGGCACGCCACG+TGG	-	chr3_3:65008540-65008559	Msa0464810:intron	65.0%
	CACTTCCACGTGGCGTGCCT+CGG	+	chr3_3:65008548-65008567	None:intergenic	65.0%
	ATGTTCCGAGGCACGCCACG+TGG	-	chr3_3:65008540-65008559	Msa0464810:intron	65.0%
	CACTTCCACGTGGCGTGCCT+CGG	+	chr3_3:65008548-65008567	None:intergenic	65.0%
	CGAGGCACGCCACGTGGAAG+TGG	-	chr3_3:65008546-65008565	Msa0464810:intron	70.0%
	CGAGGCACGCCACGTGGAAG+TGG	-	chr3_3:65008546-65008565	Msa0464810:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	65007989	65010142	65007989	ID=Msa0464810;Name=Msa0464810
chr3_3	mRNA	65007989	65010142	65007989	ID=Msa0464810-mRNA-1;Parent=Msa0464810;Name=Msa0464810-mRNA-1;_AED=0.04;_eAED=0.04;_QI=227\|1\|1\|1\|1\|1\|4\|266\|362
chr3_3	exon	65007989	65008518	65007989	ID=Msa0464810-mRNA-1:exon:14410;Parent=Msa0464810-mRNA-1
chr3_3	exon	65008677	65008817	65008677	ID=Msa0464810-mRNA-1:exon:14409;Parent=Msa0464810-mRNA-1
chr3_3	exon	65008911	65009146	65008911	ID=Msa0464810-mRNA-1:exon:14408;Parent=Msa0464810-mRNA-1
chr3_3	exon	65009468	65010142	65009468	ID=Msa0464810-mRNA-1:exon:14407;Parent=Msa0464810-mRNA-1
chr3_3	five_prime_UTR	65009916	65010142	65009916	ID=Msa0464810-mRNA-1:five_prime_utr;Parent=Msa0464810-mRNA-1
chr3_3	CDS	65009468	65009915	65009468	ID=Msa0464810-mRNA-1:cds;Parent=Msa0464810-mRNA-1
chr3_3	CDS	65008911	65009146	65008911	ID=Msa0464810-mRNA-1:cds;Parent=Msa0464810-mRNA-1
chr3_3	CDS	65008677	65008817	65008677	ID=Msa0464810-mRNA-1:cds;Parent=Msa0464810-mRNA-1
chr3_3	CDS	65008255	65008518	65008255	ID=Msa0464810-mRNA-1:cds;Parent=Msa0464810-mRNA-1
chr3_3	three_prime_UTR	65007989	65008254	65007989	ID=Msa0464810-mRNA-1:three_prime_utr;Parent=Msa0464810-mRNA-1

Gene Sequence

>Msa0464810

ATGGAAACTAAGAAACTATTGTTGATTGTTCTGTCCATTGCTTTGGTTCTTGAAGTATCCGAGAGCTTCGATTTCCAAGATAAAGATGTGTCCTCGGACAAAAGCTTGTGGGACTTATACGAGAGATGGAGGAGTCACCACACGGTTTCTCGGAACCTTGACGAGAAACAAAAGCGCTTTAACGTGTTCAAATCAAATGTGATACATGTCCACAACACTAACAAGATGGATAAGCCTTACAAGTTGAAACTTAACAAGTTTGCCGACATGACCAACCATGAATTTAGGACTACCTATGCTGGATCAAAGGTTAATCACCACAGAATGTTCCGAGGCACGCCACGTGGAAGTGGAACATTCATGTATGAAAATTTTACCAAAGCTCCTGCTTCTGTTGATTGGAGAAAGAAAGGTGCTGTCACTGAGGTCAAGGATCAAGGCCAATGTGGTAGTTGCTGGGCTTTTTCAACTGTTGTAGCTGTTGAAGGCATAAACCAAATAAAGACAAATAGGCTAGTGCCTTTGTCTGAACAAGAGCTTATAGATTGTGACAATCAAGAAAATCAAGGATGCAATGGTGGGCTAATGGAATATGCTTTTGAGTACATCAAGCAAAAAGGTGGCATAACAACAGAAAGCTATTACCCTTACACAGCAAATGATGGATCATGTGATGCAACAAAAGAAAATGTCCCAGCTGTGTCAATTGATGGTCATGAGACTGTTCCGGCTAACGATGAAGATGCTTTGCTCAAAGCTGTTGCCAACCAACCTGTATCCGTAGCCATAGATGCCGGAGGATCTGATTTCCAGTTTTACTCAGAGGGAGTATTTAGTGGCGATTGTGGTAAAGAGCTAAATCATGGTGTAGCAATAGTAGGATATGGAACAACCGTTGATGGAACTAATTATTGGATTGTGAGGAACTCATGGGGAGCGGAATGGGGTGAACAAGGTTACATCAGAATGCAAAGGAATGTATCTAACAAGGAAGGACTTTGTGGCATAGCTATGGAAGCTTCCTACCCAGTTAAAAACTCCTCCAAAAATCCAGCAGGACCTTTATCATCTACCAAGGATGAGCTTTGA

Protein sequence

>Msa0464810

METKKLLLIVLSIALVLEVSESFDFQDKDVSSDKSLWDLYERWRSHHTVSRNLDEKQKRFNVFKSNVIHVHNTNKMDKPYKLKLNKFADMTNHEFRTTYAGSKVNHHRMFRGTPRGSGTFMYENFTKAPASVDWRKKGAVTEVKDQGQCGSCWAFSTVVAVEGINQIKTNRLVPLSEQELIDCDNQENQGCNGGLMEYAFEYIKQKGGITTESYYPYTANDGSCDATKENVPAVSIDGHETVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFSGDCGKELNHGVAIVGYGTTVDGTNYWIVRNSWGAEWGEQGYIRMQRNVSNKEGLCGIAMEASYPVKNSSKNPAGPLSSTKDEL*