AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0476710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0476710 MtrunA17_Chr3g0137121 98.592 213 3 0 1 213 1 213 2.11e-159 439
Msa0476710 MtrunA17_Chr1g0149791 72.811 217 55 2 1 213 1 217 1.48e-117 333
Msa0476710 MtrunA17_Chr1g0154681 64.024 164 59 0 14 177 39 202 1.15e-80 241
Msa0476710 MtrunA17_Chr4g0002531 67.516 157 50 1 15 171 25 180 9.75e-78 233
Msa0476710 MtrunA17_Chr1g0162711 57.303 178 76 0 12 189 36 213 5.75e-77 231
Msa0476710 MtrunA17_Chr4g0055821 57.143 154 66 0 9 162 14 167 7.09e-69 210
Msa0476710 MtrunA17_Chr5g0399541 60.140 143 57 0 19 161 25 167 4.26e-68 208
Msa0476710 MtrunA17_Chr4g0063261 59.441 143 58 0 19 161 31 173 9.28e-64 197
Msa0476710 MtrunA17_Chr2g0306871 55.797 138 61 0 24 161 33 170 2.89e-60 188
Msa0476710 MtrunA17_Chr1g0202701 33.333 195 103 8 19 192 4 192 3.40e-23 93.2
Msa0476710 MtrunA17_Chr6g0468771 40.000 110 63 3 36 143 34 142 3.74e-21 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0476710 AT5G25170.1 77.528 178 37 2 6 181 8 184 9.27e-101 291
Msa0476710 AT2G25190.1 70.588 170 50 0 10 179 10 179 8.23e-94 274
Msa0476710 AT1G80690.1 66.279 172 52 2 15 181 13 183 8.68e-81 241
Msa0476710 AT1G80690.2 65.341 176 51 3 15 181 13 187 8.34e-79 236
Msa0476710 AT1G47740.1 55.024 209 81 2 14 209 66 274 2.18e-78 236
Msa0476710 AT1G47740.2 55.024 209 81 2 14 209 66 274 2.18e-78 236
Msa0476710 AT1G47740.3 55.024 209 81 2 14 209 66 274 2.18e-78 236
Msa0476710 AT4G31980.1 66.061 165 56 0 1 165 1 165 1.31e-77 246
Msa0476710 AT5G25170.2 74.101 139 34 1 45 181 2 140 1.68e-72 218
Msa0476710 AT4G17486.1 50.000 182 87 2 13 194 22 199 8.66e-71 215
Msa0476710 AT5G47310.1 49.438 178 90 0 17 194 28 205 1.06e-68 211
Msa0476710 AT4G17486.2 57.692 130 55 0 13 142 22 151 1.47e-61 191
Msa0476710 AT4G25660.1 40.000 110 63 3 36 143 34 142 2.42e-20 86.3
Msa0476710 AT4G25680.1 40.000 110 63 3 36 143 34 142 4.80e-20 85.5
Msa0476710 AT3G07090.2 31.783 129 72 3 19 139 8 128 1.47e-12 65.5
Msa0476710 AT3G07090.1 31.783 129 72 3 19 139 8 128 1.47e-12 65.5

Find 77 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTAACGGTTATGCTTAT+TGG 0.129676 3_3:+80572682 Msa0476710:CDS
TTTCTTTGTTTCTCCATTCT+TGG 0.134095 3_3:+80574576 Msa0476710:three_prime_UTR
CCAATAAGCATAACCGTTAA+TGG 0.223848 3_3:-80572682 None:intergenic
GGTCTTGGTGTCTACCATTC+AGG 0.255831 3_3:+80572708 Msa0476710:CDS
GATGTTTGTTACAAATTAAC+TGG 0.283670 3_3:+80573051 Msa0476710:CDS
GCAGTTCATGATGTTGAATA+TGG 0.287319 3_3:+80572823 Msa0476710:intron
CATGATGTTGAATATGGTTT+TGG 0.298326 3_3:+80572829 Msa0476710:CDS
TAAGCAACATAAGATGATTC+AGG 0.309185 3_3:+80574411 Msa0476710:three_prime_UTR
GTAGAGCAGTGTACCTTTAC+TGG 0.318469 3_3:+80574664 Msa0476710:three_prime_UTR
GTAGTATTTGTGGTCAATTA+AGG 0.319018 3_3:-80572447 None:intergenic
TAGAATTGCAATAGCTACTA+TGG 0.320408 3_3:+80574485 Msa0476710:three_prime_UTR
ATGTTGTTTAAATTTGTAAC+AGG 0.349757 3_3:+80572600 Msa0476710:CDS
ATATTATGAGTTGATGAAAA+TGG 0.355546 3_3:+80574705 Msa0476710:three_prime_UTR
AACGGTTATGCTTATTGGCT+TGG 0.365043 3_3:+80572687 Msa0476710:CDS
TGTAAACCCAGGACAGTTCT+TGG 0.374934 3_3:-80572893 None:intergenic
TGAAAGAATTGTAGTATTTG+TGG 0.376510 3_3:-80572457 None:intergenic
TGATGAACCTTGAGGATTAG+AGG 0.385937 3_3:-80574277 None:intergenic
TGAAGCAAAGTAGGCACTTT+CGG 0.387010 3_3:+80572333 Msa0476710:five_prime_UTR
TATGCTTATTGGCTTGGTCT+TGG 0.396983 3_3:+80572693 Msa0476710:CDS
AAAAGATATTCGAGTATTCA+TGG 0.405447 3_3:+80572957 Msa0476710:CDS
GTGTGTGAAATGAAATTAGA+AGG 0.410923 3_3:-80572523 None:intergenic
AAGGAGAAATTGAATTATGA+AGG 0.411151 3_3:-80572504 None:intergenic
TCATAGACATTAAGATAAAC+AGG 0.419830 3_3:-80572651 None:intergenic
TATGATCTCACACCCATTAA+CGG 0.439304 3_3:+80572669 Msa0476710:CDS
ACCTTTGTTTCATTTAAGCC+AGG 0.453538 3_3:-80574177 None:intergenic
CAATAAGCATAACCGTTAAT+GGG 0.454345 3_3:-80572681 None:intergenic
TTAAATTTGTAACAGGTCCT+AGG 0.459653 3_3:+80572607 Msa0476710:CDS
AGATAAACAGGAACGGTGCC+TGG 0.463481 3_3:-80572639 None:intergenic
GTGCAACCCAAGAACTGTCC+TGG 0.468233 3_3:+80572886 Msa0476710:CDS
GTATTTCCATTTCCCAGTAA+AGG 0.468868 3_3:-80574677 None:intergenic
AGTGTACCTTTACTGGGAAA+TGG 0.474787 3_3:+80574671 Msa0476710:three_prime_UTR
GTAAACCCAGGACAGTTCTT+GGG 0.475359 3_3:-80572892 None:intergenic
TTTATTGGTACAACGGATCT+GGG 0.492318 3_3:+80572931 Msa0476710:CDS
GTGCCTGGTTTATTCTTCCT+AGG 0.495057 3_3:-80572624 None:intergenic
AAATCAGAAGTCAGTCGAGC+AGG 0.509704 3_3:+80574244 Msa0476710:CDS
GCACATGAGAATGACACAAC+GGG 0.512307 3_3:+80572853 Msa0476710:CDS
TTACTTTACTGTTAATGTTG+TGG 0.518105 3_3:-80574343 None:intergenic
AAGTAGGCACTTTCGGCCGC+CGG 0.520024 3_3:+80572340 Msa0476710:five_prime_UTR
ATAGTATATGTGACTGTGGA+TGG 0.524162 3_3:-80572385 None:intergenic
TGTTACCTTGAACTCCTGAA+TGG 0.525614 3_3:-80572722 None:intergenic
GCTTAAATGAAACAAAGGTT+CGG 0.527769 3_3:+80574181 Msa0476710:CDS
GTCTACCATTCAGGAGTTCA+AGG 0.527892 3_3:+80572717 Msa0476710:CDS
GAAGGTGGGTGAAGCAAAGT+AGG 0.527980 3_3:+80572324 Msa0476710:five_prime_UTR
GTGAATCGACTTGCAAGACT+TGG 0.529761 3_3:+80573093 Msa0476710:CDS
TGCACTCATAGAGTAAAGAC+AGG 0.533347 3_3:+80572236 None:intergenic
AAAGAAAAGGAGTGGAAGGT+GGG 0.540527 3_3:+80572310 Msa0476710:five_prime_UTR
AAAAGAAAAGGAGTGGAAGG+TGG 0.542628 3_3:+80572309 Msa0476710:five_prime_UTR
CTGGAAAGTCTATTCCTCGA+TGG 0.545239 3_3:+80573070 Msa0476710:CDS
AAACTAGCTCAAGAGTACTC+TGG 0.546204 3_3:+80572982 Msa0476710:CDS
AAGCAACATAAGATGATTCA+GGG 0.554705 3_3:+80574412 Msa0476710:three_prime_UTR
AGGAGAAATTGAATTATGAA+GGG 0.566240 3_3:-80572503 None:intergenic
TGGAAAGTCTATTCCTCGAT+GGG 0.567577 3_3:+80573071 Msa0476710:CDS
TGCAACCCAAGAACTGTCCT+GGG 0.570444 3_3:+80572887 Msa0476710:CDS
TTGCAATAGCTACTATGGAA+TGG 0.572548 3_3:+80574490 Msa0476710:three_prime_UTR
CCCTGGCTTAAATGAAACAA+AGG 0.575438 3_3:+80574176 Msa0476710:CDS
CCTTTGTTTCATTTAAGCCA+GGG 0.576422 3_3:-80574176 None:intergenic
CACGAGAGGAACAATTATCA+TGG 0.579884 3_3:+80572261 Msa0476710:five_prime_UTR
TAGGCACTTTCGGCCGCCGG+TGG 0.583249 3_3:+80572343 Msa0476710:five_prime_UTR
GATGCTTCCTCTAATCCTCA+AGG 0.583321 3_3:+80574270 Msa0476710:CDS
GTATTTGTGGTCAATTAAGG+AGG 0.583459 3_3:-80572444 None:intergenic
CAATGTCTTGATGAACCTTG+AGG 0.603172 3_3:-80574285 None:intergenic
TAGAGCAGTGTACCTTTACT+GGG 0.604888 3_3:+80574665 Msa0476710:three_prime_UTR
CTTTGTTTCATTTAAGCCAG+GGG 0.610009 3_3:-80574175 None:intergenic
TGTTAACTAGGTTGTGCTGA+AGG 0.625185 3_3:-80572555 None:intergenic
GACATTAAGATAAACAGGAA+CGG 0.631998 3_3:-80572646 None:intergenic
GGAAATAGTATATGTGACTG+TGG 0.642092 3_3:-80572389 None:intergenic
CAGCATGTATCGGTATCCAC+CGG 0.647500 3_3:-80572359 None:intergenic
AGTAAAGACAGGGACACGAG+AGG 0.649534 3_3:+80572247 None:intergenic
TGGATGGATGCAGCATGTAT+CGG 0.658330 3_3:-80572369 None:intergenic
AGCACATGAGAATGACACAA+CGG 0.687804 3_3:+80572852 Msa0476710:CDS
CATGTATCGGTATCCACCGG+CGG 0.689603 3_3:-80572356 None:intergenic
GCAAGTCGATTCACCCATCG+AGG 0.697146 3_3:-80573084 None:intergenic
GGTCCTAGGAAGAATAAACC+AGG 0.698084 3_3:+80572621 Msa0476710:CDS
TTTGTTTCATTTAAGCCAGG+GGG 0.701964 3_3:-80574174 None:intergenic
TCGGCAAGATACATCCGACA+AGG 0.712843 3_3:+80574200 Msa0476710:CDS
GCTTTGATTAAAGATGATCG+AGG 0.732245 3_3:-80574312 None:intergenic
GCACTCATAGAGTAAAGACA+GGG 0.739650 3_3:+80572237 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATAAAAAATAAAAAGAAA+AGG + chr3_3:80572297-80572316 Msa0476710:five_prime_UTR 10.0%
!! TATTTAAAAACTTTAAATGT+TGG + chr3_3:80573298-80573317 Msa0476710:intron 10.0%
!!! TAAATATTTTGATGTTAACT+AGG - chr3_3:80572570-80572589 None:intergenic 15.0%
!! TAAACCTTCAAATATAATTA+AGG - chr3_3:80574136-80574155 None:intergenic 15.0%
!! AAAAATAAAAAGAAAAGGAG+TGG + chr3_3:80572302-80572321 Msa0476710:five_prime_UTR 20.0%
!!! ATGTTGTTTAAATTTGTAAC+AGG + chr3_3:80572600-80572619 Msa0476710:CDS 20.0%
!!! AATCTACATACATTAGTTTA+AGG - chr3_3:80572767-80572786 None:intergenic 20.0%
!!! TTCAGAAAATCGATTTTTAT+TGG + chr3_3:80572916-80572935 Msa0476710:CDS 20.0%
!! ATTACAAAAGTGAAGAAAAT+TGG - chr3_3:80573326-80573345 None:intergenic 20.0%
!! TTCTCCTTAATTATATTTGA+AGG + chr3_3:80574129-80574148 Msa0476710:intron 20.0%
! CCAAGAAAAAGAATAATAGT+GGG - chr3_3:80572413-80572432 None:intergenic 25.0%
! TGAAAGAATTGTAGTATTTG+TGG - chr3_3:80572460-80572479 None:intergenic 25.0%
! AGGAGAAATTGAATTATGAA+GGG - chr3_3:80572506-80572525 None:intergenic 25.0%
! AAGGAGAAATTGAATTATGA+AGG - chr3_3:80572507-80572526 None:intergenic 25.0%
! TCATAGACATTAAGATAAAC+AGG - chr3_3:80572654-80572673 None:intergenic 25.0%
!! GTTTAAGGTTAATTAAAAGC+AGG - chr3_3:80572752-80572771 None:intergenic 25.0%
!!! ATCGATTTTTATTGGTACAA+CGG + chr3_3:80572924-80572943 Msa0476710:CDS 25.0%
! AAAAGATATTCGAGTATTCA+TGG + chr3_3:80572957-80572976 Msa0476710:CDS 25.0%
!! ACAAAAATGGTTGCAATTTT+TGG - chr3_3:80573028-80573047 None:intergenic 25.0%
! AACAAACATCATCACAAAAA+TGG - chr3_3:80573041-80573060 None:intergenic 25.0%
!! GATGTTTGTTACAAATTAAC+TGG + chr3_3:80573051-80573070 Msa0476710:CDS 25.0%
!!! CTACGTCTATTTCTAAATAT+AGG - chr3_3:80573364-80573383 None:intergenic 25.0%
!!! ATGTACTTGTTTTATGTGAT+TGG + chr3_3:80574031-80574050 Msa0476710:intron 25.0%
! TTACTTTACTGTTAATGTTG+TGG - chr3_3:80574346-80574365 None:intergenic 25.0%
!!! AAACTCAAACACTATTTTAG+AGG - chr3_3:80574387-80574406 None:intergenic 25.0%
!! TGCTTTCTTTCTTTTATGAA+TGG + chr3_3:80574549-80574568 Msa0476710:three_prime_UTR 25.0%
!! GCTTTCTTTCTTTTATGAAT+GGG + chr3_3:80574550-80574569 Msa0476710:three_prime_UTR 25.0%
ATAAAAAGAAAAGGAGTGGA+AGG + chr3_3:80572306-80572325 Msa0476710:five_prime_UTR 30.0%
! CCCACTATTATTCTTTTTCT+TGG + chr3_3:80572410-80572429 Msa0476710:five_prime_UTR 30.0%
GCCAAGAAAAAGAATAATAG+TGG - chr3_3:80572414-80572433 None:intergenic 30.0%
GTAGTATTTGTGGTCAATTA+AGG - chr3_3:80572450-80572469 None:intergenic 30.0%
GTGTGTGAAATGAAATTAGA+AGG - chr3_3:80572526-80572545 None:intergenic 30.0%
TTAAATTTGTAACAGGTCCT+AGG + chr3_3:80572607-80572626 Msa0476710:CDS 30.0%
GACATTAAGATAAACAGGAA+CGG - chr3_3:80572649-80572668 None:intergenic 30.0%
CAATAAGCATAACCGTTAAT+GGG - chr3_3:80572684-80572703 None:intergenic 30.0%
! CATGATGTTGAATATGGTTT+TGG + chr3_3:80572829-80572848 Msa0476710:CDS 30.0%
AATTGCTGTATTTGTGTCTT+TGG + chr3_3:80573214-80573233 Msa0476710:intron 30.0%
AATTCTTAAGATGGTCCTTA+GGG - chr3_3:80573267-80573286 None:intergenic 30.0%
TGGACAAAGAATTCTTAAGA+TGG - chr3_3:80573276-80573295 None:intergenic 30.0%
! ACTTTTGTAATTTAGAGACC+AGG + chr3_3:80573335-80573354 Msa0476710:intron 30.0%
! CTTTTGTAATTTAGAGACCA+GGG + chr3_3:80573336-80573355 Msa0476710:intron 30.0%
ATTTCTAAATATAGGAACCC+TGG - chr3_3:80573356-80573375 None:intergenic 30.0%
! ATTAGTCTATCTAAGGTTTG+GGG - chr3_3:80573431-80573450 None:intergenic 30.0%
! CATTAGTCTATCTAAGGTTT+GGG - chr3_3:80573432-80573451 None:intergenic 30.0%
! TCATTAGTCTATCTAAGGTT+TGG - chr3_3:80573433-80573452 None:intergenic 30.0%
! CACATTCATTAGTCTATCTA+AGG - chr3_3:80573438-80573457 None:intergenic 30.0%
CTTAGATAGACTAATGAATG+TGG + chr3_3:80573436-80573455 Msa0476710:intron 30.0%
TAATCTTTACTCACATGCTT+AGG - chr3_3:80573529-80573548 None:intergenic 30.0%
TGAGTAAAGATTACTCTCTT+GGG + chr3_3:80573536-80573555 Msa0476710:intron 30.0%
TAATATTAGCTCATGTGCTT+AGG - chr3_3:80573588-80573607 None:intergenic 30.0%
AGCTAATATTACTCTCTGTT+GGG + chr3_3:80573597-80573616 Msa0476710:intron 30.0%
! ATTGGATTGGATCATCAATT+CGG + chr3_3:80573637-80573656 Msa0476710:intron 30.0%
!! TAAGATTTTGGGTGAATATG+TGG - chr3_3:80573684-80573703 None:intergenic 30.0%
!!! TCTTTTTTGGTTCATAGTGT+CGG - chr3_3:80573826-80573845 None:intergenic 30.0%
! AGTTGTTTCACTTGTTTACA+AGG - chr3_3:80574077-80574096 None:intergenic 30.0%
GCTTAAATGAAACAAAGGTT+CGG + chr3_3:80574181-80574200 Msa0476710:CDS 30.0%
! TTCTTTTTCTTCAACCTTGT+CGG - chr3_3:80574217-80574236 None:intergenic 30.0%
AGGTTGAAGAAAAAGAAAAG+AGG + chr3_3:80574220-80574239 Msa0476710:CDS 30.0%
TAAGCAACATAAGATGATTC+AGG + chr3_3:80574411-80574430 Msa0476710:three_prime_UTR 30.0%
AAGCAACATAAGATGATTCA+GGG + chr3_3:80574412-80574431 Msa0476710:three_prime_UTR 30.0%
TAGAATTGCAATAGCTACTA+TGG + chr3_3:80574485-80574504 Msa0476710:three_prime_UTR 30.0%
! TTTCTTTGTTTCTCCATTCT+TGG + chr3_3:80574576-80574595 Msa0476710:three_prime_UTR 30.0%
TGAAAAATACAGTCCAAGAA+TGG - chr3_3:80574592-80574611 None:intergenic 30.0%
! TTGGACTGTATTTTTCATTC+TGG + chr3_3:80574595-80574614 Msa0476710:three_prime_UTR 30.0%
ATAGTATATGTGACTGTGGA+TGG - chr3_3:80572388-80572407 None:intergenic 35.0%
GGAAATAGTATATGTGACTG+TGG - chr3_3:80572392-80572411 None:intergenic 35.0%
GTATTTGTGGTCAATTAAGG+AGG - chr3_3:80572447-80572466 None:intergenic 35.0%
TATGATCTCACACCCATTAA+CGG + chr3_3:80572669-80572688 Msa0476710:CDS 35.0%
CCAATAAGCATAACCGTTAA+TGG - chr3_3:80572685-80572704 None:intergenic 35.0%
CCATTAACGGTTATGCTTAT+TGG + chr3_3:80572682-80572701 Msa0476710:CDS 35.0%
GCAGTTCATGATGTTGAATA+TGG + chr3_3:80572823-80572842 Msa0476710:intron 35.0%
! GATTTTCTGAATGTAAACCC+AGG - chr3_3:80572907-80572926 None:intergenic 35.0%
! TTTTATTGGTACAACGGATC+TGG + chr3_3:80572930-80572949 Msa0476710:CDS 35.0%
TTTATTGGTACAACGGATCT+GGG + chr3_3:80572931-80572950 Msa0476710:CDS 35.0%
ATTCTTAAGATGGTCCTTAG+GGG - chr3_3:80573266-80573285 None:intergenic 35.0%
GAATTCTTAAGATGGTCCTT+AGG - chr3_3:80573268-80573287 None:intergenic 35.0%
! TTAGTCTATCTAAGGTTTGG+GGG - chr3_3:80573430-80573449 None:intergenic 35.0%
ACTAATGAATGTGGCTTAGT+TGG + chr3_3:80573445-80573464 Msa0476710:intron 35.0%
TAAAGTTCTTTGTCACTACC+CGG - chr3_3:80573498-80573517 None:intergenic 35.0%
GTGAGTAAAGATTACTCTCT+TGG + chr3_3:80573535-80573554 Msa0476710:intron 35.0%
GAGTAAAGATTACTCTCTTG+GGG + chr3_3:80573537-80573556 Msa0476710:intron 35.0%
GAGCTAATATTACTCTCTGT+TGG + chr3_3:80573596-80573615 Msa0476710:intron 35.0%
! ATTGATGATCCAATCCAATG+AGG - chr3_3:80573636-80573655 None:intergenic 35.0%
TCACCCAAAATCTTAAAGCA+CGG + chr3_3:80573689-80573708 Msa0476710:intron 35.0%
!!! ACTCCGTGCTTTAAGATTTT+GGG - chr3_3:80573695-80573714 None:intergenic 35.0%
!!! AACTCCGTGCTTTAAGATTT+TGG - chr3_3:80573696-80573715 None:intergenic 35.0%
ATCTTAAAGCACGGAGTTTA+TGG + chr3_3:80573698-80573717 Msa0476710:intron 35.0%
AGTTAAAGTCTCATGTAGGA+TGG - chr3_3:80573760-80573779 None:intergenic 35.0%
TGTGAGTTAAAGTCTCATGT+AGG - chr3_3:80573764-80573783 None:intergenic 35.0%
!!! GCACAAAAGTCTGTCTTTTT+TGG - chr3_3:80573839-80573858 None:intergenic 35.0%
! TTCAAGCATGTGTTTGTTGA+GGG - chr3_3:80573987-80574006 None:intergenic 35.0%
! ATTCAAGCATGTGTTTGTTG+AGG - chr3_3:80573988-80574007 None:intergenic 35.0%
TTTGTTTCATTTAAGCCAGG+GGG - chr3_3:80574177-80574196 None:intergenic 35.0%
! CTTTGTTTCATTTAAGCCAG+GGG - chr3_3:80574178-80574197 None:intergenic 35.0%
! CCTTTGTTTCATTTAAGCCA+GGG - chr3_3:80574179-80574198 None:intergenic 35.0%
! ACCTTTGTTTCATTTAAGCC+AGG - chr3_3:80574180-80574199 None:intergenic 35.0%
GCTTTGATTAAAGATGATCG+AGG - chr3_3:80574315-80574334 None:intergenic 35.0%
TTGCAATAGCTACTATGGAA+TGG + chr3_3:80574490-80574509 Msa0476710:three_prime_UTR 35.0%
GTATTTCCATTTCCCAGTAA+AGG - chr3_3:80574680-80574699 None:intergenic 35.0%
CACGAGAGGAACAATTATCA+TGG + chr3_3:80572261-80572280 Msa0476710:five_prime_UTR 40.0%
AAAAGAAAAGGAGTGGAAGG+TGG + chr3_3:80572309-80572328 Msa0476710:five_prime_UTR 40.0%
AAAGAAAAGGAGTGGAAGGT+GGG + chr3_3:80572310-80572329 Msa0476710:five_prime_UTR 40.0%
TGAAGCAAAGTAGGCACTTT+CGG + chr3_3:80572333-80572352 Msa0476710:five_prime_UTR 40.0%
TGTTAACTAGGTTGTGCTGA+AGG - chr3_3:80572558-80572577 None:intergenic 40.0%
! AACGGTTATGCTTATTGGCT+TGG + chr3_3:80572687-80572706 Msa0476710:CDS 40.0%
!! TATGCTTATTGGCTTGGTCT+TGG + chr3_3:80572693-80572712 Msa0476710:CDS 40.0%
TGTTACCTTGAACTCCTGAA+TGG - chr3_3:80572725-80572744 None:intergenic 40.0%
AGCACATGAGAATGACACAA+CGG + chr3_3:80572852-80572871 Msa0476710:CDS 40.0%
!! AAACTAGCTCAAGAGTACTC+TGG + chr3_3:80572982-80573001 Msa0476710:CDS 40.0%
TGGAAAGTCTATTCCTCGAT+GGG + chr3_3:80573071-80573090 Msa0476710:CDS 40.0%
GGTGGAGATAGTAGATTTCA+TGG - chr3_3:80573245-80573264 None:intergenic 40.0%
! TAGTCTATCTAAGGTTTGGG+GGG - chr3_3:80573429-80573448 None:intergenic 40.0%
TAAGAAGCAGAACACATTCG+TGG + chr3_3:80573469-80573488 Msa0476710:intron 40.0%
AAGAAGCAGAACACATTCGT+GGG + chr3_3:80573470-80573489 Msa0476710:intron 40.0%
TTCGGTTAAGACAAGTGAGT+TGG + chr3_3:80573655-80573674 Msa0476710:intron 40.0%
! CAGTCTTATAGTTAGTCCCA+AGG + chr3_3:80573800-80573819 Msa0476710:intron 40.0%
CCCTGGCTTAAATGAAACAA+AGG + chr3_3:80574176-80574195 Msa0476710:CDS 40.0%
TGATGAACCTTGAGGATTAG+AGG - chr3_3:80574280-80574299 None:intergenic 40.0%
CAATGTCTTGATGAACCTTG+AGG - chr3_3:80574288-80574307 None:intergenic 40.0%
TAGAGCAGTGTACCTTTACT+GGG + chr3_3:80574665-80574684 Msa0476710:three_prime_UTR 40.0%
AGTGTACCTTTACTGGGAAA+TGG + chr3_3:80574671-80574690 Msa0476710:three_prime_UTR 40.0%
TGGATGGATGCAGCATGTAT+CGG - chr3_3:80572372-80572391 None:intergenic 45.0%
GGTCCTAGGAAGAATAAACC+AGG + chr3_3:80572621-80572640 Msa0476710:CDS 45.0%
GTGCCTGGTTTATTCTTCCT+AGG - chr3_3:80572627-80572646 None:intergenic 45.0%
GTCTACCATTCAGGAGTTCA+AGG + chr3_3:80572717-80572736 Msa0476710:CDS 45.0%
GCACATGAGAATGACACAAC+GGG + chr3_3:80572853-80572872 Msa0476710:CDS 45.0%
GTAAACCCAGGACAGTTCTT+GGG - chr3_3:80572895-80572914 None:intergenic 45.0%
TGTAAACCCAGGACAGTTCT+TGG - chr3_3:80572896-80572915 None:intergenic 45.0%
CTGGAAAGTCTATTCCTCGA+TGG + chr3_3:80573070-80573089 Msa0476710:CDS 45.0%
!! GTGAATCGACTTGCAAGACT+TGG + chr3_3:80573093-80573112 Msa0476710:CDS 45.0%
ATCTACTATCTCCACCCCTA+AGG + chr3_3:80573249-80573268 Msa0476710:intron 45.0%
AGAAGCAGAACACATTCGTG+GGG + chr3_3:80573471-80573490 Msa0476710:intron 45.0%
CATGTAGGATGGAAGAGTTG+AGG - chr3_3:80573749-80573768 None:intergenic 45.0%
AGAGCTCTGCAGTCAAAATG+TGG + chr3_3:80573953-80573972 Msa0476710:intron 45.0%
AAATCAGAAGTCAGTCGAGC+AGG + chr3_3:80574244-80574263 Msa0476710:CDS 45.0%
GATGCTTCCTCTAATCCTCA+AGG + chr3_3:80574270-80574289 Msa0476710:CDS 45.0%
GTAGAGCAGTGTACCTTTAC+TGG + chr3_3:80574664-80574683 Msa0476710:three_prime_UTR 45.0%
!!! ATTTAAAGTTTTTAAATAAT+TGG - chr3_3:80573296-80573315 None:intergenic 5.0%
GAAGGTGGGTGAAGCAAAGT+AGG + chr3_3:80572324-80572343 Msa0476710:five_prime_UTR 50.0%
! CAGCATGTATCGGTATCCAC+CGG - chr3_3:80572362-80572381 None:intergenic 50.0%
AGATAAACAGGAACGGTGCC+TGG - chr3_3:80572642-80572661 None:intergenic 50.0%
!! GGTCTTGGTGTCTACCATTC+AGG + chr3_3:80572708-80572727 Msa0476710:CDS 50.0%
TGCAACCCAAGAACTGTCCT+GGG + chr3_3:80572887-80572906 Msa0476710:CDS 50.0%
CTTAAGATGGTCCTTAGGGG+TGG - chr3_3:80573263-80573282 None:intergenic 50.0%
GAAGCAGAACACATTCGTGG+GGG + chr3_3:80573472-80573491 Msa0476710:intron 50.0%
TTACTCTCTGTTGGGCCATG+AGG + chr3_3:80573605-80573624 Msa0476710:intron 50.0%
TACTCTCTGTTGGGCCATGA+GGG + chr3_3:80573606-80573625 Msa0476710:intron 50.0%
!! TGGTTCATAGTGTCGGCCTT+GGG - chr3_3:80573819-80573838 None:intergenic 50.0%
!! TTGGTTCATAGTGTCGGCCT+TGG - chr3_3:80573820-80573839 None:intergenic 50.0%
TCGGCAAGATACATCCGACA+AGG + chr3_3:80574200-80574219 Msa0476710:CDS 50.0%
! CATGTATCGGTATCCACCGG+CGG - chr3_3:80572359-80572378 None:intergenic 55.0%
GTGCAACCCAAGAACTGTCC+TGG + chr3_3:80572886-80572905 Msa0476710:CDS 55.0%
! GCAAGTCGATTCACCCATCG+AGG - chr3_3:80573087-80573106 None:intergenic 55.0%
ACTCTCTGTTGGGCCATGAG+GGG + chr3_3:80573607-80573626 Msa0476710:intron 55.0%
AAGTAGGCACTTTCGGCCGC+CGG + chr3_3:80572340-80572359 Msa0476710:five_prime_UTR 60.0%
CAGAACACATTCGTGGGGGC+CGG + chr3_3:80573476-80573495 Msa0476710:intron 60.0%
AGAACACATTCGTGGGGGCC+GGG + chr3_3:80573477-80573496 Msa0476710:intron 60.0%
CTCTCTGTTGGGCCATGAGG+GGG + chr3_3:80573608-80573627 Msa0476710:intron 60.0%
TCCAATGAGGCTCCCCCTCA+TGG - chr3_3:80573623-80573642 None:intergenic 60.0%
GAGGGGGAGCCTCATTGGAT+TGG + chr3_3:80573624-80573643 Msa0476710:intron 60.0%
TGCAACTGCGTTCTTCCCCC+TGG + chr3_3:80574159-80574178 Msa0476710:CDS 60.0%
GCCATGAGGGGGAGCCTCAT+TGG + chr3_3:80573619-80573638 Msa0476710:intron 65.0%
TAGGCACTTTCGGCCGCCGG+TGG + chr3_3:80572343-80572362 Msa0476710:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 80572248 80574724 80572248 ID=Msa0476710;Name=Msa0476710
chr3_3 mRNA 80572248 80574724 80572248 ID=Msa0476710-mRNA-1;Parent=Msa0476710;Name=Msa0476710-mRNA-1;_AED=0.22;_eAED=0.22;_QI=352|1|1|1|1|1|3|359|213
chr3_3 exon 80572248 80572738 80572248 ID=Msa0476710-mRNA-1:exon:22088;Parent=Msa0476710-mRNA-1
chr3_3 exon 80572827 80573114 80572827 ID=Msa0476710-mRNA-1:exon:22089;Parent=Msa0476710-mRNA-1
chr3_3 exon 80574151 80574724 80574151 ID=Msa0476710-mRNA-1:exon:22090;Parent=Msa0476710-mRNA-1
chr3_3 five_prime_UTR 80572248 80572599 80572248 ID=Msa0476710-mRNA-1:five_prime_utr;Parent=Msa0476710-mRNA-1
chr3_3 CDS 80572600 80572738 80572600 ID=Msa0476710-mRNA-1:cds;Parent=Msa0476710-mRNA-1
chr3_3 CDS 80572827 80573114 80572827 ID=Msa0476710-mRNA-1:cds;Parent=Msa0476710-mRNA-1
chr3_3 CDS 80574151 80574365 80574151 ID=Msa0476710-mRNA-1:cds;Parent=Msa0476710-mRNA-1
chr3_3 three_prime_UTR 80574366 80574724 80574366 ID=Msa0476710-mRNA-1:three_prime_utr;Parent=Msa0476710-mRNA-1
Gene Sequence

>Msa0476710

ATGTTGTTTAAATTTGTAACAGGTCCTAGGAAGAATAAACCAGGCACCGTTCCTGTTTATCTTAATGTCTATGATCTCACACCCATTAACGGTTATGCTTATTGGCTTGGTCTTGGTGTCTACCATTCAGGAGTTCAAGTTCATGATGTTGAATATGGTTTTGGAGCACATGAGAATGACACAACGGGAATTTTTGAAGTGCAACCCAAGAACTGTCCTGGGTTTACATTCAGAAAATCGATTTTTATTGGTACAACGGATCTGGGTACAAAAGATATTCGAGTATTCATGGAGAAACTAGCTCAAGAGTACTCTGGAAATTCGTATCATCTTATTTCCAAAAATTGCAACCATTTTTGTGATGATGTTTGTTACAAATTAACTGGAAAGTCTATTCCTCGATGGGTGAATCGACTTGCAAGACTTGGTTTATTCTGCAACTGCGTTCTTCCCCCTGGCTTAAATGAAACAAAGGTTCGGCAAGATACATCCGACAAGGTTGAAGAAAAAGAAAAGAGGAAAATCAGAAGTCAGTCGAGCAGGTGTGATGCTTCCTCTAATCCTCAAGGTTCATCAAGACATTGCTTTCCTCGATCATCTTTAATCAAAGCTTTGTCAACCACAACATTAACAGTAAAGTAA
Protein sequence

>Msa0476710

MLFKFVTGPRKNKPGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHDVEYGFGAHENDTTGIFEVQPKNCPGFTFRKSIFIGTTDLGTKDIRVFMEKLAQEYSGNSYHLISKNCNHFCDDVCYKLTGKSIPRWVNRLARLGLFCNCVLPPGLNETKVRQDTSDKVEEKEKRKIRSQSSRCDASSNPQGSSRHCFPRSSLIKALSTTTLTVK*