AlfalfaGEDB | Msa0512360

M. sativa cultivar zhongmu-4 / Msa0512360

KEGG: K16281|K16285
GO: GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006066 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009628 GO:0009651 GO:0009687 GO:0009719 GO:0009725 GO:0009739 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010498 GO:0010646 GO:0010647 GO:0016567 GO:0016740 GO:0019538 GO:0019752 GO:0019787 GO:0019941 GO:0023051 GO:0023056 GO:0030163 GO:0032446 GO:0032787 GO:0033993 GO:0036211 GO:0042221 GO:0042493 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044464 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080134 GO:0080167 GO:0140096 GO:1901419 GO:1901421 GO:1901564 GO:1901565 GO:1901575 GO:1901615 GO:1901698 GO:1901700 GO:1902644 GO:1905957 GO:1905959
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0512360	XP_003601512.2	95.455	154	7	0	1	154	1	154	1.11e-96	285

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0512360	sp\|Q9SI09\|XERIC_ARATH	57.419	155	59	3	1	149	1	154	3.67e-52	166

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0512360	B7FHG6	95.455	154	7	0	1	154	1	154	5.30e-97	285

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0007390	Msa0512360	0.802345	5.861729e-49	-8.615850e-47
Msa0026280	Msa0512360	-0.821774	3.377053e-53	-8.615850e-47
Msa0074140	Msa0512360	-0.824222	9.072374e-54	-8.615850e-47
Msa0074180	Msa0512360	-0.832351	9.935837e-56	-8.615850e-47
Msa0116590	Msa0512360	-0.814414	1.559725e-51	-8.615850e-47
Msa0415950	Msa0512360	-0.802466	5.537309e-49	-8.615850e-47
Msa0438480	Msa0512360	0.948202	1.505809e-106	-8.615850e-47
Msa0512360	Msa0651840	0.807680	4.489754e-50	-8.615850e-47
Msa0512360	Msa0694120	0.823055	1.701716e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0512360	MtrunA17_Chr3g0120681	95.455	154	7	0	1	154	1	154	1.02e-100	285
Msa0512360	MtrunA17_Chr3g0123581	34.437	151	89	5	1	143	1	149	8.56e-22	85.5
Msa0512360	MtrunA17_Chr8g0387291	33.113	151	89	5	5	154	2	141	1.10e-16	72.4
Msa0512360	MtrunA17_Chr5g0397351	27.972	143	82	3	10	148	5	130	2.49e-14	65.9
Msa0512360	MtrunA17_Chr7g0248101	38.028	71	44	0	77	147	68	138	2.82e-14	66.6
Msa0512360	MtrunA17_Chr3g0124531	43.590	78	36	3	72	149	49	118	4.50e-14	65.5
Msa0512360	MtrunA17_Chr4g0069751	29.605	152	81	5	1	146	1	132	3.53e-13	63.5
Msa0512360	MtrunA17_Chr4g0069761	41.667	72	36	1	81	146	3	74	1.64e-12	60.1
Msa0512360	MtrunA17_Chr8g0348551	45.098	51	28	0	99	149	92	142	1.57e-11	59.3
Msa0512360	MtrunA17_Chr1g0212451	41.176	68	37	2	81	148	65	129	3.34e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0512360	AT2G04240.1	57.419	155	59	3	1	149	1	154	3.73e-53	166
Msa0512360	AT2G04240.2	57.419	155	59	3	1	149	1	154	3.73e-53	166
Msa0512360	AT1G24580.1	38.255	149	70	4	1	145	1	131	1.72e-20	82.0
Msa0512360	AT1G67856.1	31.724	145	87	1	1	145	1	133	2.88e-20	81.3
Msa0512360	AT3G61460.1	27.586	145	96	3	8	149	6	144	3.41e-18	77.0
Msa0512360	AT4G11360.1	30.657	137	80	5	18	152	16	139	2.64e-14	66.6
Msa0512360	AT2G01150.1	52.941	51	23	1	98	148	73	122	1.76e-13	64.3
Msa0512360	AT2G01150.3	52.941	51	23	1	98	148	99	148	2.57e-13	64.3
Msa0512360	AT4G00305.1	34.066	91	52	2	58	148	38	120	3.10e-13	63.2
Msa0512360	AT2G01150.2	52.941	51	23	1	98	148	122	171	4.11e-13	64.3
Msa0512360	AT1G15100.1	44.615	65	35	1	85	149	72	135	5.30e-13	63.2
Msa0512360	AT4G11370.1	29.365	126	76	4	25	149	24	137	2.04e-12	61.6
Msa0512360	AT1G63840.1	41.429	70	35	3	81	148	74	139	3.52e-12	61.2
Msa0512360	AT5G41400.1	39.437	71	41	2	81	149	86	156	4.99e-12	60.8
Msa0512360	AT3G43430.1	47.368	57	22	1	99	147	83	139	2.44e-11	58.9
Msa0512360	AT3G60220.1	43.243	74	35	2	76	149	100	166	9.03e-11	58.9

Find 29 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTTCTCCAAACACACTTTA+TGG	0.118411	3_4:-62121012	None:intergenic
CCTTATAATGCCTTTGAATA+TGG	0.192958	3_4:-62120723	None:intergenic
CAATAGTTTATCTCTGATTC+AGG	0.259823	3_4:-62120968	None:intergenic
TGGAGAAGTGGTTGGATTAT+TGG	0.271795	3_4:+62121026	Msa0512360:CDS
GCATCATCTTCAGGAATTAA+AGG	0.292620	3_4:-62121076	None:intergenic
CCATATTCAAAGGCATTATA+AGG	0.374596	3_4:+62120723	Msa0512360:CDS
ACATTACATGTCCACTTTGT+AGG	0.377000	3_4:+62121050	Msa0512360:CDS
AAGCAAGATGCATCATCTTC+AGG	0.389175	3_4:-62121085	None:intergenic
AATCATCTGAAGGACTTAGA+TGG	0.425512	3_4:-62120838	None:intergenic
CACACTGAACAATCATGTTC+AGG	0.425873	3_4:-62120929	None:intergenic
TCTTGTGAAGGAGAGGAAGA+TGG	0.468958	3_4:-62120779	None:intergenic
GTTATAGTCTTGTGAAGGAG+AGG	0.499474	3_4:-62120786	None:intergenic
GAAGCAGGACACCAACACTT+AGG	0.520971	3_4:+62120876	Msa0512360:CDS
AGGACTATCCTACACATTGT+TGG	0.523738	3_4:+62120743	Msa0512360:CDS
AACACTCCTTCTGATTGTGC+TGG	0.524508	3_4:-62120665	None:intergenic
ATAAAGTGTGTTTGGAGAAG+TGG	0.526466	3_4:+62121014	Msa0512360:CDS
TCATTGAAGAGTTCAGAAGC+AGG	0.540082	3_4:+62120861	Msa0512360:CDS
ATAAACTATTGCATATCTTG+TGG	0.556021	3_4:+62120980	Msa0512360:CDS
TGAGGGTTATAGTCTTGTGA+AGG	0.563911	3_4:-62120791	None:intergenic
AGTGTGTTTGGAGAAGTGGT+TGG	0.571451	3_4:+62121018	Msa0512360:CDS
TCTTCAATGAAATCATCTGA+AGG	0.575424	3_4:-62120848	None:intergenic
ATGTCAATATCCATATTCAA+AGG	0.593951	3_4:+62120713	Msa0512360:CDS
ATTGTGCTGGAAGACTTGAT+AGG	0.597735	3_4:-62120652	None:intergenic
ATTAAAGGACTCCTACAAAG+TGG	0.606868	3_4:-62121061	None:intergenic
AGGTTTGACAGTGTGTGTAG+TGG	0.612919	3_4:+62120896	Msa0512360:CDS
CAATGATACCAACAATGTGT+AGG	0.618977	3_4:-62120751	None:intergenic
ACACTGTCAAACCTAAGTGT+TGG	0.628752	3_4:-62120887	None:intergenic
AGTCTTCCAGCACAATCAGA+AGG	0.634115	3_4:+62120659	Msa0512360:CDS
TTTGAATATGGATATTGACA+TGG	0.689378	3_4:-62120711	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTTGAATATGGATATTGACA+TGG	-	chr3_4:62120714-62120733	None:intergenic	25.0%
!	ATGTCAATATCCATATTCAA+AGG	+	chr3_4:62120713-62120732	Msa0512360:CDS	25.0%
!	ATAAACTATTGCATATCTTG+TGG	+	chr3_4:62120980-62120999	Msa0512360:CDS	25.0%
	CCTTATAATGCCTTTGAATA+TGG	-	chr3_4:62120726-62120745	None:intergenic	30.0%
!!	CCATATTCAAAGGCATTATA+AGG	+	chr3_4:62120723-62120742	Msa0512360:CDS	30.0%
	TCTTCAATGAAATCATCTGA+AGG	-	chr3_4:62120851-62120870	None:intergenic	30.0%
	CAATAGTTTATCTCTGATTC+AGG	-	chr3_4:62120971-62120990	None:intergenic	30.0%
!	TGTTTTCCATAAAGTGTGTT+TGG	+	chr3_4:62121006-62121025	Msa0512360:CDS	30.0%
	CAATGATACCAACAATGTGT+AGG	-	chr3_4:62120754-62120773	None:intergenic	35.0%
!!	ATGACTCAGATGAGTTTTGA+GGG	-	chr3_4:62120811-62120830	None:intergenic	35.0%
!	AATGACTCAGATGAGTTTTG+AGG	-	chr3_4:62120812-62120831	None:intergenic	35.0%
	AATCATCTGAAGGACTTAGA+TGG	-	chr3_4:62120841-62120860	None:intergenic	35.0%
	ACTTCTCCAAACACACTTTA+TGG	-	chr3_4:62121015-62121034	None:intergenic	35.0%
	ATAAAGTGTGTTTGGAGAAG+TGG	+	chr3_4:62121014-62121033	Msa0512360:CDS	35.0%
	ACATTACATGTCCACTTTGT+AGG	+	chr3_4:62121050-62121069	Msa0512360:CDS	35.0%
	ATTAAAGGACTCCTACAAAG+TGG	-	chr3_4:62121064-62121083	None:intergenic	35.0%
	GCATCATCTTCAGGAATTAA+AGG	-	chr3_4:62121079-62121098	None:intergenic	35.0%
	ATTGTGCTGGAAGACTTGAT+AGG	-	chr3_4:62120655-62120674	None:intergenic	40.0%
	AGGACTATCCTACACATTGT+TGG	+	chr3_4:62120743-62120762	Msa0512360:CDS	40.0%
!!	GTTATAGTCTTGTGAAGGAG+AGG	-	chr3_4:62120789-62120808	None:intergenic	40.0%
!!	TGAGGGTTATAGTCTTGTGA+AGG	-	chr3_4:62120794-62120813	None:intergenic	40.0%
	TCATTGAAGAGTTCAGAAGC+AGG	+	chr3_4:62120861-62120880	Msa0512360:CDS	40.0%
	ACACTGTCAAACCTAAGTGT+TGG	-	chr3_4:62120890-62120909	None:intergenic	40.0%
	CACACTGAACAATCATGTTC+AGG	-	chr3_4:62120932-62120951	None:intergenic	40.0%
	TGGAGAAGTGGTTGGATTAT+TGG	+	chr3_4:62121026-62121045	Msa0512360:CDS	40.0%
	AAGCAAGATGCATCATCTTC+AGG	-	chr3_4:62121088-62121107	None:intergenic	40.0%
	AGTCTTCCAGCACAATCAGA+AGG	+	chr3_4:62120659-62120678	Msa0512360:CDS	45.0%
	AACACTCCTTCTGATTGTGC+TGG	-	chr3_4:62120668-62120687	None:intergenic	45.0%
	TCTTGTGAAGGAGAGGAAGA+TGG	-	chr3_4:62120782-62120801	None:intergenic	45.0%
	AGGTTTGACAGTGTGTGTAG+TGG	+	chr3_4:62120896-62120915	Msa0512360:CDS	45.0%
	AGTGTGTTTGGAGAAGTGGT+TGG	+	chr3_4:62121018-62121037	Msa0512360:CDS	45.0%
	GAAGCAGGACACCAACACTT+AGG	+	chr3_4:62120876-62120895	Msa0512360:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	62120647	62121111	62120647	ID=Msa0512360;Name=Msa0512360
chr3_4	mRNA	62120647	62121111	62120647	ID=Msa0512360-mRNA-1;Parent=Msa0512360;Name=Msa0512360-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|154
chr3_4	exon	62120647	62121111	62120647	ID=Msa0512360-mRNA-1:exon:15325;Parent=Msa0512360-mRNA-1
chr3_4	CDS	62120647	62121111	62120647	ID=Msa0512360-mRNA-1:cds;Parent=Msa0512360-mRNA-1

Gene Sequence

>Msa0512360

ATGGGCCTATCAAGTCTTCCAGCACAATCAGAAGGAGTGTTATGCATCATTCTAGTAAACACTGCCATGTCAATATCCATATTCAAAGGCATTATAAGGACTATCCTACACATTGTTGGTATCATTGCTTCACCATCTTCCTCTCCTTCACAAGACTATAACCCTCAAAACTCATCTGAGTCATTTGAAATCCATCTAAGTCCTTCAGATGATTTCATTGAAGAGTTCAGAAGCAGGACACCAACACTTAGGTTTGACAGTGTGTGTAGTGGCTGTAAAGAACCTGAACATGATTGTTCAGTGTGTCTCACTCAATTTGAACCTGAATCAGAGATAAACTATTGCATATCTTGTGGACATGTTTTCCATAAAGTGTGTTTGGAGAAGTGGTTGGATTATTGGAACATTACATGTCCACTTTGTAGGAGTCCTTTAATTCCTGAAGATGATGCATCTTGCTTATGA

Protein sequence

>Msa0512360

MGLSSLPAQSEGVLCIILVNTAMSISIFKGIIRTILHIVGIIASPSSSPSQDYNPQNSSESFEIHLSPSDDFIEEFRSRTPTLRFDSVCSGCKEPEHDCSVCLTQFEPESEINYCISCGHVFHKVCLEKWLDYWNITCPLCRSPLIPEDDASCL*