AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0517560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517560 MtrunA17_Chr3g0128921 99.541 218 1 0 8 225 5 222 6.39e-164 451
Msa0517560 MtrunA17_Chr2g0325151 99.087 219 2 0 8 226 5 223 1.28e-163 450
Msa0517560 MtrunA17_Chr3g0108371 92.523 214 16 0 8 221 5 218 9.36e-149 412
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517560 AT1G26910.1 89.252 214 23 0 8 221 5 218 2.67e-144 402
Msa0517560 AT1G14320.1 88.785 214 24 0 8 221 5 218 5.31e-143 398
Msa0517560 AT1G66580.1 87.442 215 27 0 8 222 5 219 3.79e-141 394
Msa0517560 AT1G26910.2 88.398 181 21 0 41 221 1 181 2.27e-118 335
Msa0517560 AT1G14320.2 77.660 94 19 1 130 221 31 124 1.50e-46 150
Msa0517560 AT1G14320.2 97.436 39 1 0 41 79 1 39 2.03e-20 84.0

Find 84 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATAGAGATTTGGTTTATT+TGG 0.151129 3_4:+69262445 Msa0517560:three_prime_UTR
GGTTCAATTTCTTTGAAAAT+TGG 0.171559 3_4:+69262545 Msa0517560:three_prime_UTR
TTCTCACATTAAATACTAAA+AGG 0.270230 3_4:-69262412 None:intergenic
ATGCTTTCCACTTGAGAGTT+AGG 0.278634 3_4:+69261438 Msa0517560:CDS
TTCATCCATTCCATGTTCTT+AGG 0.295164 3_4:+69261462 Msa0517560:CDS
CTTTGACATGTATTTGTTGC+AGG 0.312261 3_4:-69261402 None:intergenic
CCAAATAAACCAAATCTCTA+TGG 0.318302 3_4:-69262445 None:intergenic
CAGAAACGTGACTTTGGATA+TGG 0.332410 3_4:-69261236 None:intergenic
ATTTGCAAGTGAAGTAGATT+TGG 0.351457 3_4:-69262493 None:intergenic
ACCATTGGCCAACCGTCAAC+CGG 0.355147 3_4:+69262172 Msa0517560:CDS
GTTTCCTTTCTGTGTTCATC+TGG 0.355959 3_4:+69261325 Msa0517560:CDS
CTGTGTTCATCTGGTCAGTT+GGG 0.370061 3_4:+69261334 Msa0517560:CDS
ATTTGACGGTAACACCTTGC+AGG 0.370519 3_4:-69261203 None:intergenic
ATTCGGTGATTCTCACGAAA+TGG 0.374153 3_4:+69262523 Msa0517560:three_prime_UTR
AACATCATAAATCCTGATCT+TGG 0.385450 3_4:-69261276 None:intergenic
TCTGTGTTCATCTGGTCAGT+TGG 0.393251 3_4:+69261333 Msa0517560:CDS
ATCAGGCATAATTCTGTTCT+CGG 0.407151 3_4:-69261889 None:intergenic
TTTGTGCTAGAGTGGCTATT+GGG 0.420431 3_4:+69261564 Msa0517560:CDS
GCAGTCAGGAAAGCTCTTCC+CGG 0.421225 3_4:-69262191 None:intergenic
ACTGGTATGAGAGGGGCGTT+TGG 0.425175 3_4:+69261527 Msa0517560:CDS
TTTCATGTGCGGGAGCTGAT+AGG 0.426261 3_4:+69261498 Msa0517560:CDS
TTGCAGCTTCTTGGATGCCA+TGG 0.430564 3_4:+69262149 Msa0517560:intron
TGCTAGAGTGGCTATTGGGC+AGG 0.435686 3_4:+69261568 Msa0517560:CDS
TGCTTTCCACTTGAGAGTTA+GGG 0.441216 3_4:+69261439 Msa0517560:CDS
GGAAAGCTCTTCCCGGTTGA+CGG 0.444695 3_4:-69262184 None:intergenic
GTTTGTGCTAGAGTGGCTAT+TGG 0.453432 3_4:+69261563 Msa0517560:CDS
CATGTCAAAGTTTGCAGGAA+AGG 0.453517 3_4:+69261415 Msa0517560:CDS
AGAATCAAGGGCAAAGAGAT+TGG 0.454933 3_4:-69262654 None:intergenic
TTCGGTGATTCTCACGAAAT+GGG 0.455075 3_4:+69262524 Msa0517560:three_prime_UTR
ACCTTAGCATTCACACCATC+AGG 0.455380 3_4:-69261906 None:intergenic
AGCAGCTTCAAGTGCCTCAC+TGG 0.456981 3_4:-69261372 None:intergenic
ACTTGCAAATCTCACTGATT+CGG 0.458990 3_4:+69262506 Msa0517560:three_prime_UTR
CCCGGTTGACGGTTGGCCAA+TGG 0.460642 3_4:-69262173 None:intergenic
ATGGCTTGTTCTTAATTTGA+CGG 0.461186 3_4:-69261217 None:intergenic
CCACGGCAGAAACGTGACTT+TGG 0.463875 3_4:-69261242 None:intergenic
CGTGCTAAGTTTAAGTTCCC+TGG 0.464822 3_4:+69261650 Msa0517560:CDS
CCTCATTACAGCTGGCAGTC+AGG 0.466220 3_4:-69262205 None:intergenic
AAATACATGTCAAAGTTTGC+AGG 0.470834 3_4:+69261410 Msa0517560:CDS
TTGAGGAAAAGAAAGAATCA+AGG 0.477618 3_4:-69262667 None:intergenic
ACATCATAAATCCTGATCTT+GGG 0.479634 3_4:-69261275 None:intergenic
TCTTCTTTCTGTTCGTTGCA+AGG 0.480717 3_4:+69261592 Msa0517560:CDS
TAAATCCTGATCTTGGGATC+AGG 0.483079 3_4:-69261269 None:intergenic
CTCAAGGTCGCTCTTGCAAA+CGG 0.488436 3_4:-69262344 None:intergenic
TGCCAGCTGTAATGAGGCTT+AGG 0.494501 3_4:+69262211 Msa0517560:CDS
ATGAGTAAGTAAGAAAAGTT+CGG 0.495313 3_4:-69262470 None:intergenic
TCTTGGATGCCATGGACCAT+TGG 0.496449 3_4:+69262157 Msa0517560:CDS
CCTGACTGCCAGCTGTAATG+AGG 0.497594 3_4:+69262205 Msa0517560:CDS
ATTTCTTTGAAAATTGGTGA+TGG 0.500390 3_4:+69262551 Msa0517560:three_prime_UTR
AACAAGATGCTTTCATGTGC+GGG 0.504380 3_4:+69261488 Msa0517560:CDS
AGCTCTTCCCGGTTGACGGT+TGG 0.505552 3_4:-69262180 None:intergenic
GTGTTCCTGATCCCAAGATC+AGG 0.511204 3_4:+69261264 Msa0517560:CDS
GTCAAAAGATCATTGTTAGC+AGG 0.521890 3_4:+69261675 Msa0517560:CDS
CAACAACAGCCATCATGCTC+AGG 0.528429 3_4:+69261616 Msa0517560:CDS
AGATCATTGTTAGCAGGAAG+TGG 0.531763 3_4:+69261681 Msa0517560:CDS
AGCCTAAGCCTCATTACAGC+TGG 0.535691 3_4:-69262213 None:intergenic
GACGGTTGGCCAATGGTCCA+TGG 0.540431 3_4:-69262166 None:intergenic
AAGATCAGGATTTATGATGT+TGG 0.540872 3_4:+69261278 Msa0517560:CDS
ACAAGGTGTTTGTGCTAGAG+TGG 0.563189 3_4:+69261556 Msa0517560:CDS
CCATTGGCCAACCGTCAACC+GGG 0.565470 3_4:+69262173 Msa0517560:CDS
GGCTTCAGACTGGTATGAGA+GGG 0.565692 3_4:+69261519 Msa0517560:CDS
TCATCTGGTCAGTTGGGAGA+AGG 0.572889 3_4:+69261340 Msa0517560:CDS
GGAGAGCCTCCTGAGCATGA+TGG 0.573200 3_4:-69261625 None:intergenic
CTGACCAGATGAACACAGAA+AGG 0.573863 3_4:-69261329 None:intergenic
TGAGGAAAAGAAAGAATCAA+GGG 0.574614 3_4:-69262666 None:intergenic
GATGAACCCTAACTCTCAAG+TGG 0.578245 3_4:-69261445 None:intergenic
CCAAAGTCACGTTTCTGCCG+TGG 0.586608 3_4:+69261242 Msa0517560:CDS
TAAGATTAATCATCAACTCA+AGG 0.592706 3_4:-69262360 None:intergenic
GTGAGGCACTTGAAGCTGCT+AGG 0.594487 3_4:+69261375 Msa0517560:CDS
CAACAAGATGCTTTCATGTG+CGG 0.597854 3_4:+69261487 Msa0517560:CDS
GATGTTGGCATGAAGAAGAA+AGG 0.601420 3_4:+69261293 Msa0517560:CDS
TTGATCCTAAGAACATGGAA+TGG 0.602625 3_4:-69261467 None:intergenic
TTAACTTCAGATAATCAGCT+CGG 0.606395 3_4:-69261863 None:intergenic
AGGCTTCAGACTGGTATGAG+AGG 0.607126 3_4:+69261518 Msa0517560:CDS
CAACAGCCATCATGCTCAGG+AGG 0.612805 3_4:+69261619 Msa0517560:CDS
TCTTGTTGATCCTAAGAACA+TGG 0.613434 3_4:-69261472 None:intergenic
GCCTGATGGTGTGAATGCTA+AGG 0.639133 3_4:+69261905 Msa0517560:CDS
GAGAACAGAATTATGCCTGA+TGG 0.652808 3_4:+69261891 Msa0517560:CDS
GGAGCTGATAGGCTTCAGAC+TGG 0.660599 3_4:+69261509 Msa0517560:CDS
ACTCTAGCACAAACACCTTG+TGG 0.661898 3_4:-69261554 None:intergenic
GGGGCGTTTGGAAAGCCACA+AGG 0.670359 3_4:+69261539 Msa0517560:CDS
GCTTCAGACTGGTATGAGAG+GGG 0.696136 3_4:+69261520 Msa0517560:CDS
GAAGGAAAATGTGTCCAGTG+AGG 0.706232 3_4:+69261358 Msa0517560:CDS
GGAACTTAAACTTAGCACGA+CGG 0.721521 3_4:-69261646 None:intergenic
TCTTGGGATCAGGAACACCA+CGG 0.737353 3_4:-69261259 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATCTTATATTTTGTTA+TGG + chr3_4:69262373-69262392 Msa0517560:three_prime_UTR 10.0%
!!! ATTAATCTTATATTTTGTTA+TGG + chr3_4:69262373-69262392 Msa0517560:three_prime_UTR 10.0%
!! TATTTAATGTGAGAAAAAAA+AGG + chr3_4:69262420-69262439 Msa0517560:three_prime_UTR 15.0%
!! TATTTAATGTGAGAAAAAAA+AGG + chr3_4:69262420-69262439 Msa0517560:three_prime_UTR 15.0%
!! CAATGAATATAGTTAAAACA+TGG - chr3_4:69261804-69261823 None:intergenic 20.0%
!!! ATCTTAGTTTGGAATATATT+GGG + chr3_4:69262016-69262035 Msa0517560:intron 20.0%
!!! AAAAAGAACTTTAAGTTGAA+AGG - chr3_4:69262123-69262142 None:intergenic 20.0%
!! TTCTCACATTAAATACTAAA+AGG - chr3_4:69262415-69262434 None:intergenic 20.0%
!! CAATGAATATAGTTAAAACA+TGG - chr3_4:69261804-69261823 None:intergenic 20.0%
!!! ATCTTAGTTTGGAATATATT+GGG + chr3_4:69262016-69262035 Msa0517560:intron 20.0%
!!! AAAAAGAACTTTAAGTTGAA+AGG - chr3_4:69262123-69262142 None:intergenic 20.0%
!! TTCTCACATTAAATACTAAA+AGG - chr3_4:69262415-69262434 None:intergenic 20.0%
!! AAAACATGGATTGAAGTAAA+AGG - chr3_4:69261790-69261809 None:intergenic 25.0%
!!! TTGTTTTAAATGCTTCTTCA+GGG + chr3_4:69261824-69261843 Msa0517560:intron 25.0%
! TAACTTGAATCATCTTAGTT+TGG + chr3_4:69262005-69262024 Msa0517560:intron 25.0%
!! CATCTTAGTTTGGAATATAT+TGG + chr3_4:69262015-69262034 Msa0517560:intron 25.0%
!! TCTTAGTTTGGAATATATTG+GGG + chr3_4:69262017-69262036 Msa0517560:intron 25.0%
!!! GTTAGTTTTGTCTAAGTAAT+CGG + chr3_4:69262097-69262116 Msa0517560:intron 25.0%
! TAAGATTAATCATCAACTCA+AGG - chr3_4:69262363-69262382 None:intergenic 25.0%
! ATGAGTAAGTAAGAAAAGTT+CGG - chr3_4:69262473-69262492 None:intergenic 25.0%
!! GGTTCAATTTCTTTGAAAAT+TGG + chr3_4:69262545-69262564 Msa0517560:three_prime_UTR 25.0%
!! ATTTCTTTGAAAATTGGTGA+TGG + chr3_4:69262551-69262570 Msa0517560:three_prime_UTR 25.0%
!! AAAACATGGATTGAAGTAAA+AGG - chr3_4:69261790-69261809 None:intergenic 25.0%
!!! TTGTTTTAAATGCTTCTTCA+GGG + chr3_4:69261824-69261843 Msa0517560:intron 25.0%
! TAACTTGAATCATCTTAGTT+TGG + chr3_4:69262005-69262024 Msa0517560:intron 25.0%
!! CATCTTAGTTTGGAATATAT+TGG + chr3_4:69262015-69262034 Msa0517560:intron 25.0%
!! TCTTAGTTTGGAATATATTG+GGG + chr3_4:69262017-69262036 Msa0517560:intron 25.0%
!!! GTTAGTTTTGTCTAAGTAAT+CGG + chr3_4:69262097-69262116 Msa0517560:intron 25.0%
! TAAGATTAATCATCAACTCA+AGG - chr3_4:69262363-69262382 None:intergenic 25.0%
! ATGAGTAAGTAAGAAAAGTT+CGG - chr3_4:69262473-69262492 None:intergenic 25.0%
!! GGTTCAATTTCTTTGAAAAT+TGG + chr3_4:69262545-69262564 Msa0517560:three_prime_UTR 25.0%
!! ATTTCTTTGAAAATTGGTGA+TGG + chr3_4:69262551-69262570 Msa0517560:three_prime_UTR 25.0%
!! TTTTATTTATAGGACCTGCA+AGG + chr3_4:69261189-69261208 Msa0517560:CDS 30.0%
!! ATGGCTTGTTCTTAATTTGA+CGG - chr3_4:69261220-69261239 None:intergenic 30.0%
ACATCATAAATCCTGATCTT+GGG - chr3_4:69261278-69261297 None:intergenic 30.0%
AACATCATAAATCCTGATCT+TGG - chr3_4:69261279-69261298 None:intergenic 30.0%
AAGATCAGGATTTATGATGT+TGG + chr3_4:69261278-69261297 Msa0517560:CDS 30.0%
AAATACATGTCAAAGTTTGC+AGG + chr3_4:69261410-69261429 Msa0517560:CDS 30.0%
! TGCTAACAATGATCTTTTGA+CGG - chr3_4:69261676-69261695 None:intergenic 30.0%
GTGGTAAGTATTTGTTCTAT+TGG + chr3_4:69261700-69261719 Msa0517560:intron 30.0%
!!! GTTGTTTTAAATGCTTCTTC+AGG + chr3_4:69261823-69261842 Msa0517560:intron 30.0%
!! TGTTTTAAATGCTTCTTCAG+GGG + chr3_4:69261825-69261844 Msa0517560:intron 30.0%
TTAACTTCAGATAATCAGCT+CGG - chr3_4:69261866-69261885 None:intergenic 30.0%
AGAAACAAATATCCAACATG+CGG - chr3_4:69261961-69261980 None:intergenic 30.0%
AATCTGTTTGAACACTCAAT+TGG + chr3_4:69262062-69262081 Msa0517560:intron 30.0%
AAAAAGGTTCCATAGAGATT+TGG + chr3_4:69262436-69262455 Msa0517560:three_prime_UTR 30.0%
CCAAATAAACCAAATCTCTA+TGG - chr3_4:69262448-69262467 None:intergenic 30.0%
! CCATAGAGATTTGGTTTATT+TGG + chr3_4:69262445-69262464 Msa0517560:three_prime_UTR 30.0%
ATTTGCAAGTGAAGTAGATT+TGG - chr3_4:69262496-69262515 None:intergenic 30.0%
!! TTTTATTTATAGGACCTGCA+AGG + chr3_4:69261189-69261208 Msa0517560:CDS 30.0%
!! ATGGCTTGTTCTTAATTTGA+CGG - chr3_4:69261220-69261239 None:intergenic 30.0%
ACATCATAAATCCTGATCTT+GGG - chr3_4:69261278-69261297 None:intergenic 30.0%
AACATCATAAATCCTGATCT+TGG - chr3_4:69261279-69261298 None:intergenic 30.0%
AAGATCAGGATTTATGATGT+TGG + chr3_4:69261278-69261297 Msa0517560:CDS 30.0%
AAATACATGTCAAAGTTTGC+AGG + chr3_4:69261410-69261429 Msa0517560:CDS 30.0%
! TGCTAACAATGATCTTTTGA+CGG - chr3_4:69261676-69261695 None:intergenic 30.0%
GTGGTAAGTATTTGTTCTAT+TGG + chr3_4:69261700-69261719 Msa0517560:intron 30.0%
!!! GTTGTTTTAAATGCTTCTTC+AGG + chr3_4:69261823-69261842 Msa0517560:intron 30.0%
!! TGTTTTAAATGCTTCTTCAG+GGG + chr3_4:69261825-69261844 Msa0517560:intron 30.0%
TTAACTTCAGATAATCAGCT+CGG - chr3_4:69261866-69261885 None:intergenic 30.0%
AGAAACAAATATCCAACATG+CGG - chr3_4:69261961-69261980 None:intergenic 30.0%
AATCTGTTTGAACACTCAAT+TGG + chr3_4:69262062-69262081 Msa0517560:intron 30.0%
AAAAAGGTTCCATAGAGATT+TGG + chr3_4:69262436-69262455 Msa0517560:three_prime_UTR 30.0%
CCAAATAAACCAAATCTCTA+TGG - chr3_4:69262448-69262467 None:intergenic 30.0%
! CCATAGAGATTTGGTTTATT+TGG + chr3_4:69262445-69262464 Msa0517560:three_prime_UTR 30.0%
ATTTGCAAGTGAAGTAGATT+TGG - chr3_4:69262496-69262515 None:intergenic 30.0%
! CTTTGACATGTATTTGTTGC+AGG - chr3_4:69261405-69261424 None:intergenic 35.0%
TTCATCCATTCCATGTTCTT+AGG + chr3_4:69261462-69261481 Msa0517560:CDS 35.0%
TTGATCCTAAGAACATGGAA+TGG - chr3_4:69261470-69261489 None:intergenic 35.0%
TCTTGTTGATCCTAAGAACA+TGG - chr3_4:69261475-69261494 None:intergenic 35.0%
GTCAAAAGATCATTGTTAGC+AGG + chr3_4:69261675-69261694 Msa0517560:CDS 35.0%
GCAAATCTACAAAGGATACA+AGG - chr3_4:69261749-69261768 None:intergenic 35.0%
TCCTTTGTAGATTTGCAGTA+TGG + chr3_4:69261753-69261772 Msa0517560:intron 35.0%
CCTTTGTAGATTTGCAGTAT+GGG + chr3_4:69261754-69261773 Msa0517560:intron 35.0%
ATCAGGCATAATTCTGTTCT+CGG - chr3_4:69261892-69261911 None:intergenic 35.0%
! GCAAAAAGTATGTCTTTAGC+AGG - chr3_4:69261985-69262004 None:intergenic 35.0%
! TTTGTGATTTTGCAGCTTCT+TGG + chr3_4:69262140-69262159 Msa0517560:intron 35.0%
! ACTTGCAAATCTCACTGATT+CGG + chr3_4:69262506-69262525 Msa0517560:three_prime_UTR 35.0%
! CTTTGACATGTATTTGTTGC+AGG - chr3_4:69261405-69261424 None:intergenic 35.0%
TTCATCCATTCCATGTTCTT+AGG + chr3_4:69261462-69261481 Msa0517560:CDS 35.0%
TTGATCCTAAGAACATGGAA+TGG - chr3_4:69261470-69261489 None:intergenic 35.0%
TCTTGTTGATCCTAAGAACA+TGG - chr3_4:69261475-69261494 None:intergenic 35.0%
GTCAAAAGATCATTGTTAGC+AGG + chr3_4:69261675-69261694 Msa0517560:CDS 35.0%
GCAAATCTACAAAGGATACA+AGG - chr3_4:69261749-69261768 None:intergenic 35.0%
TCCTTTGTAGATTTGCAGTA+TGG + chr3_4:69261753-69261772 Msa0517560:intron 35.0%
CCTTTGTAGATTTGCAGTAT+GGG + chr3_4:69261754-69261773 Msa0517560:intron 35.0%
ATCAGGCATAATTCTGTTCT+CGG - chr3_4:69261892-69261911 None:intergenic 35.0%
! GCAAAAAGTATGTCTTTAGC+AGG - chr3_4:69261985-69262004 None:intergenic 35.0%
! TTTGTGATTTTGCAGCTTCT+TGG + chr3_4:69262140-69262159 Msa0517560:intron 35.0%
! ACTTGCAAATCTCACTGATT+CGG + chr3_4:69262506-69262525 Msa0517560:three_prime_UTR 35.0%
CAGAAACGTGACTTTGGATA+TGG - chr3_4:69261239-69261258 None:intergenic 40.0%
TAAATCCTGATCTTGGGATC+AGG - chr3_4:69261272-69261291 None:intergenic 40.0%
GATGTTGGCATGAAGAAGAA+AGG + chr3_4:69261293-69261312 Msa0517560:CDS 40.0%
GTTTCCTTTCTGTGTTCATC+TGG + chr3_4:69261325-69261344 Msa0517560:CDS 40.0%
CATGTCAAAGTTTGCAGGAA+AGG + chr3_4:69261415-69261434 Msa0517560:CDS 40.0%
ATGCTTTCCACTTGAGAGTT+AGG + chr3_4:69261438-69261457 Msa0517560:CDS 40.0%
TGCTTTCCACTTGAGAGTTA+GGG + chr3_4:69261439-69261458 Msa0517560:CDS 40.0%
CAACAAGATGCTTTCATGTG+CGG + chr3_4:69261487-69261506 Msa0517560:CDS 40.0%
AACAAGATGCTTTCATGTGC+GGG + chr3_4:69261488-69261507 Msa0517560:CDS 40.0%
TTTGTGCTAGAGTGGCTATT+GGG + chr3_4:69261564-69261583 Msa0517560:CDS 40.0%
TCTTCTTTCTGTTCGTTGCA+AGG + chr3_4:69261592-69261611 Msa0517560:CDS 40.0%
GGAACTTAAACTTAGCACGA+CGG - chr3_4:69261649-69261668 None:intergenic 40.0%
AGATCATTGTTAGCAGGAAG+TGG + chr3_4:69261681-69261700 Msa0517560:CDS 40.0%
CCCATACTGCAAATCTACAA+AGG - chr3_4:69261757-69261776 None:intergenic 40.0%
!! TTTGCAGTATGGGAGTACTT+TGG + chr3_4:69261764-69261783 Msa0517560:intron 40.0%
GAGAACAGAATTATGCCTGA+TGG + chr3_4:69261891-69261910 Msa0517560:CDS 40.0%
GAATTCATTGTACCGCATGT+TGG + chr3_4:69261946-69261965 Msa0517560:intron 40.0%
! ATTCGGTGATTCTCACGAAA+TGG + chr3_4:69262523-69262542 Msa0517560:three_prime_UTR 40.0%
TTCGGTGATTCTCACGAAAT+GGG + chr3_4:69262524-69262543 Msa0517560:three_prime_UTR 40.0%
AGAATCAAGGGCAAAGAGAT+TGG - chr3_4:69262657-69262676 None:intergenic 40.0%
CAGAAACGTGACTTTGGATA+TGG - chr3_4:69261239-69261258 None:intergenic 40.0%
TAAATCCTGATCTTGGGATC+AGG - chr3_4:69261272-69261291 None:intergenic 40.0%
GATGTTGGCATGAAGAAGAA+AGG + chr3_4:69261293-69261312 Msa0517560:CDS 40.0%
GTTTCCTTTCTGTGTTCATC+TGG + chr3_4:69261325-69261344 Msa0517560:CDS 40.0%
CATGTCAAAGTTTGCAGGAA+AGG + chr3_4:69261415-69261434 Msa0517560:CDS 40.0%
ATGCTTTCCACTTGAGAGTT+AGG + chr3_4:69261438-69261457 Msa0517560:CDS 40.0%
TGCTTTCCACTTGAGAGTTA+GGG + chr3_4:69261439-69261458 Msa0517560:CDS 40.0%
CAACAAGATGCTTTCATGTG+CGG + chr3_4:69261487-69261506 Msa0517560:CDS 40.0%
AACAAGATGCTTTCATGTGC+GGG + chr3_4:69261488-69261507 Msa0517560:CDS 40.0%
TTTGTGCTAGAGTGGCTATT+GGG + chr3_4:69261564-69261583 Msa0517560:CDS 40.0%
TCTTCTTTCTGTTCGTTGCA+AGG + chr3_4:69261592-69261611 Msa0517560:CDS 40.0%
GGAACTTAAACTTAGCACGA+CGG - chr3_4:69261649-69261668 None:intergenic 40.0%
AGATCATTGTTAGCAGGAAG+TGG + chr3_4:69261681-69261700 Msa0517560:CDS 40.0%
CCCATACTGCAAATCTACAA+AGG - chr3_4:69261757-69261776 None:intergenic 40.0%
!! TTTGCAGTATGGGAGTACTT+TGG + chr3_4:69261764-69261783 Msa0517560:intron 40.0%
GAGAACAGAATTATGCCTGA+TGG + chr3_4:69261891-69261910 Msa0517560:CDS 40.0%
GAATTCATTGTACCGCATGT+TGG + chr3_4:69261946-69261965 Msa0517560:intron 40.0%
! ATTCGGTGATTCTCACGAAA+TGG + chr3_4:69262523-69262542 Msa0517560:three_prime_UTR 40.0%
TTCGGTGATTCTCACGAAAT+GGG + chr3_4:69262524-69262543 Msa0517560:three_prime_UTR 40.0%
AGAATCAAGGGCAAAGAGAT+TGG - chr3_4:69262657-69262676 None:intergenic 40.0%
ATTTGACGGTAACACCTTGC+AGG - chr3_4:69261206-69261225 None:intergenic 45.0%
CTGACCAGATGAACACAGAA+AGG - chr3_4:69261332-69261351 None:intergenic 45.0%
TCTGTGTTCATCTGGTCAGT+TGG + chr3_4:69261333-69261352 Msa0517560:CDS 45.0%
CTGTGTTCATCTGGTCAGTT+GGG + chr3_4:69261334-69261353 Msa0517560:CDS 45.0%
GAAGGAAAATGTGTCCAGTG+AGG + chr3_4:69261358-69261377 Msa0517560:CDS 45.0%
GATGAACCCTAACTCTCAAG+TGG - chr3_4:69261448-69261467 None:intergenic 45.0%
ACTCTAGCACAAACACCTTG+TGG - chr3_4:69261557-69261576 None:intergenic 45.0%
! ACAAGGTGTTTGTGCTAGAG+TGG + chr3_4:69261556-69261575 Msa0517560:CDS 45.0%
GTTTGTGCTAGAGTGGCTAT+TGG + chr3_4:69261563-69261582 Msa0517560:CDS 45.0%
! CGTGCTAAGTTTAAGTTCCC+TGG + chr3_4:69261650-69261669 Msa0517560:CDS 45.0%
! CAATGATCTTTTGACGGCCA+GGG - chr3_4:69261670-69261689 None:intergenic 45.0%
! ACAATGATCTTTTGACGGCC+AGG - chr3_4:69261671-69261690 None:intergenic 45.0%
ACCTTAGCATTCACACCATC+AGG - chr3_4:69261909-69261928 None:intergenic 45.0%
ATTTGACGGTAACACCTTGC+AGG - chr3_4:69261206-69261225 None:intergenic 45.0%
CTGACCAGATGAACACAGAA+AGG - chr3_4:69261332-69261351 None:intergenic 45.0%
TCTGTGTTCATCTGGTCAGT+TGG + chr3_4:69261333-69261352 Msa0517560:CDS 45.0%
CTGTGTTCATCTGGTCAGTT+GGG + chr3_4:69261334-69261353 Msa0517560:CDS 45.0%
GAAGGAAAATGTGTCCAGTG+AGG + chr3_4:69261358-69261377 Msa0517560:CDS 45.0%
GATGAACCCTAACTCTCAAG+TGG - chr3_4:69261448-69261467 None:intergenic 45.0%
ACTCTAGCACAAACACCTTG+TGG - chr3_4:69261557-69261576 None:intergenic 45.0%
! ACAAGGTGTTTGTGCTAGAG+TGG + chr3_4:69261556-69261575 Msa0517560:CDS 45.0%
GTTTGTGCTAGAGTGGCTAT+TGG + chr3_4:69261563-69261582 Msa0517560:CDS 45.0%
! CGTGCTAAGTTTAAGTTCCC+TGG + chr3_4:69261650-69261669 Msa0517560:CDS 45.0%
! CAATGATCTTTTGACGGCCA+GGG - chr3_4:69261670-69261689 None:intergenic 45.0%
! ACAATGATCTTTTGACGGCC+AGG - chr3_4:69261671-69261690 None:intergenic 45.0%
ACCTTAGCATTCACACCATC+AGG - chr3_4:69261909-69261928 None:intergenic 45.0%
TCTTGGGATCAGGAACACCA+CGG - chr3_4:69261262-69261281 None:intergenic 50.0%
GTGTTCCTGATCCCAAGATC+AGG + chr3_4:69261264-69261283 Msa0517560:CDS 50.0%
TCATCTGGTCAGTTGGGAGA+AGG + chr3_4:69261340-69261359 Msa0517560:CDS 50.0%
TTTCATGTGCGGGAGCTGAT+AGG + chr3_4:69261498-69261517 Msa0517560:CDS 50.0%
AGGCTTCAGACTGGTATGAG+AGG + chr3_4:69261518-69261537 Msa0517560:CDS 50.0%
GGCTTCAGACTGGTATGAGA+GGG + chr3_4:69261519-69261538 Msa0517560:CDS 50.0%
GCTTCAGACTGGTATGAGAG+GGG + chr3_4:69261520-69261539 Msa0517560:CDS 50.0%
CAACAACAGCCATCATGCTC+AGG + chr3_4:69261616-69261635 Msa0517560:CDS 50.0%
! GCCTGATGGTGTGAATGCTA+AGG + chr3_4:69261905-69261924 Msa0517560:CDS 50.0%
TTGCAGCTTCTTGGATGCCA+TGG + chr3_4:69262149-69262168 Msa0517560:intron 50.0%
TCTTGGATGCCATGGACCAT+TGG + chr3_4:69262157-69262176 Msa0517560:CDS 50.0%
TGCCAGCTGTAATGAGGCTT+AGG + chr3_4:69262211-69262230 Msa0517560:CDS 50.0%
AGCCTAAGCCTCATTACAGC+TGG - chr3_4:69262216-69262235 None:intergenic 50.0%
CTCAAGGTCGCTCTTGCAAA+CGG - chr3_4:69262347-69262366 None:intergenic 50.0%
TCTTGGGATCAGGAACACCA+CGG - chr3_4:69261262-69261281 None:intergenic 50.0%
GTGTTCCTGATCCCAAGATC+AGG + chr3_4:69261264-69261283 Msa0517560:CDS 50.0%
TCATCTGGTCAGTTGGGAGA+AGG + chr3_4:69261340-69261359 Msa0517560:CDS 50.0%
TTTCATGTGCGGGAGCTGAT+AGG + chr3_4:69261498-69261517 Msa0517560:CDS 50.0%
AGGCTTCAGACTGGTATGAG+AGG + chr3_4:69261518-69261537 Msa0517560:CDS 50.0%
GGCTTCAGACTGGTATGAGA+GGG + chr3_4:69261519-69261538 Msa0517560:CDS 50.0%
GCTTCAGACTGGTATGAGAG+GGG + chr3_4:69261520-69261539 Msa0517560:CDS 50.0%
CAACAACAGCCATCATGCTC+AGG + chr3_4:69261616-69261635 Msa0517560:CDS 50.0%
! GCCTGATGGTGTGAATGCTA+AGG + chr3_4:69261905-69261924 Msa0517560:CDS 50.0%
TTGCAGCTTCTTGGATGCCA+TGG + chr3_4:69262149-69262168 Msa0517560:intron 50.0%
TCTTGGATGCCATGGACCAT+TGG + chr3_4:69262157-69262176 Msa0517560:CDS 50.0%
TGCCAGCTGTAATGAGGCTT+AGG + chr3_4:69262211-69262230 Msa0517560:CDS 50.0%
AGCCTAAGCCTCATTACAGC+TGG - chr3_4:69262216-69262235 None:intergenic 50.0%
CTCAAGGTCGCTCTTGCAAA+CGG - chr3_4:69262347-69262366 None:intergenic 50.0%
CCACGGCAGAAACGTGACTT+TGG - chr3_4:69261245-69261264 None:intergenic 55.0%
CCAAAGTCACGTTTCTGCCG+TGG + chr3_4:69261242-69261261 Msa0517560:CDS 55.0%
AGCAGCTTCAAGTGCCTCAC+TGG - chr3_4:69261375-69261394 None:intergenic 55.0%
! GTGAGGCACTTGAAGCTGCT+AGG + chr3_4:69261375-69261394 Msa0517560:CDS 55.0%
GGAGCTGATAGGCTTCAGAC+TGG + chr3_4:69261509-69261528 Msa0517560:CDS 55.0%
!! ACTGGTATGAGAGGGGCGTT+TGG + chr3_4:69261527-69261546 Msa0517560:CDS 55.0%
TGCTAGAGTGGCTATTGGGC+AGG + chr3_4:69261568-69261587 Msa0517560:CDS 55.0%
CAACAGCCATCATGCTCAGG+AGG + chr3_4:69261619-69261638 Msa0517560:CDS 55.0%
ACCATTGGCCAACCGTCAAC+CGG + chr3_4:69262172-69262191 Msa0517560:CDS 55.0%
GGAAAGCTCTTCCCGGTTGA+CGG - chr3_4:69262187-69262206 None:intergenic 55.0%
GCAGTCAGGAAAGCTCTTCC+CGG - chr3_4:69262194-69262213 None:intergenic 55.0%
CCTCATTACAGCTGGCAGTC+AGG - chr3_4:69262208-69262227 None:intergenic 55.0%
CCTGACTGCCAGCTGTAATG+AGG + chr3_4:69262205-69262224 Msa0517560:CDS 55.0%
CCACGGCAGAAACGTGACTT+TGG - chr3_4:69261245-69261264 None:intergenic 55.0%
CCAAAGTCACGTTTCTGCCG+TGG + chr3_4:69261242-69261261 Msa0517560:CDS 55.0%
AGCAGCTTCAAGTGCCTCAC+TGG - chr3_4:69261375-69261394 None:intergenic 55.0%
! GTGAGGCACTTGAAGCTGCT+AGG + chr3_4:69261375-69261394 Msa0517560:CDS 55.0%
GGAGCTGATAGGCTTCAGAC+TGG + chr3_4:69261509-69261528 Msa0517560:CDS 55.0%
!! ACTGGTATGAGAGGGGCGTT+TGG + chr3_4:69261527-69261546 Msa0517560:CDS 55.0%
TGCTAGAGTGGCTATTGGGC+AGG + chr3_4:69261568-69261587 Msa0517560:CDS 55.0%
CAACAGCCATCATGCTCAGG+AGG + chr3_4:69261619-69261638 Msa0517560:CDS 55.0%
ACCATTGGCCAACCGTCAAC+CGG + chr3_4:69262172-69262191 Msa0517560:CDS 55.0%
GGAAAGCTCTTCCCGGTTGA+CGG - chr3_4:69262187-69262206 None:intergenic 55.0%
GCAGTCAGGAAAGCTCTTCC+CGG - chr3_4:69262194-69262213 None:intergenic 55.0%
CCTCATTACAGCTGGCAGTC+AGG - chr3_4:69262208-69262227 None:intergenic 55.0%
CCTGACTGCCAGCTGTAATG+AGG + chr3_4:69262205-69262224 Msa0517560:CDS 55.0%
! GGGGCGTTTGGAAAGCCACA+AGG + chr3_4:69261539-69261558 Msa0517560:CDS 60.0%
GGAGAGCCTCCTGAGCATGA+TGG - chr3_4:69261628-69261647 None:intergenic 60.0%
! GACGGTTGGCCAATGGTCCA+TGG - chr3_4:69262169-69262188 None:intergenic 60.0%
CCATTGGCCAACCGTCAACC+GGG + chr3_4:69262173-69262192 Msa0517560:CDS 60.0%
AGCTCTTCCCGGTTGACGGT+TGG - chr3_4:69262183-69262202 None:intergenic 60.0%
! GGGGCGTTTGGAAAGCCACA+AGG + chr3_4:69261539-69261558 Msa0517560:CDS 60.0%
GGAGAGCCTCCTGAGCATGA+TGG - chr3_4:69261628-69261647 None:intergenic 60.0%
! GACGGTTGGCCAATGGTCCA+TGG - chr3_4:69262169-69262188 None:intergenic 60.0%
CCATTGGCCAACCGTCAACC+GGG + chr3_4:69262173-69262192 Msa0517560:CDS 60.0%
AGCTCTTCCCGGTTGACGGT+TGG - chr3_4:69262183-69262202 None:intergenic 60.0%
! CCCGGTTGACGGTTGGCCAA+TGG - chr3_4:69262176-69262195 None:intergenic 65.0%
! CCCGGTTGACGGTTGGCCAA+TGG - chr3_4:69262176-69262195 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 69261182 69262679 69261182 ID=Msa0517560;Name=Msa0517560
chr3_4 mRNA 69261182 69262679 69261182 ID=Msa0517560-mRNA-1;Parent=Msa0517560;Name=Msa0517560-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|1|0.66|1|1|1|3|447|226
chr3_4 exon 69261182 69261702 69261182 ID=Msa0517560-mRNA-1:exon:18380;Parent=Msa0517560-mRNA-1
chr3_4 exon 69261845 69261926 69261845 ID=Msa0517560-mRNA-1:exon:18381;Parent=Msa0517560-mRNA-1
chr3_4 exon 69262155 69262679 69262155 ID=Msa0517560-mRNA-1:exon:18382;Parent=Msa0517560-mRNA-1
chr3_4 CDS 69261182 69261702 69261182 ID=Msa0517560-mRNA-1:cds;Parent=Msa0517560-mRNA-1
chr3_4 CDS 69261845 69261926 69261845 ID=Msa0517560-mRNA-1:cds;Parent=Msa0517560-mRNA-1
chr3_4 CDS 69262155 69262232 69262155 ID=Msa0517560-mRNA-1:cds;Parent=Msa0517560-mRNA-1
chr3_4 three_prime_UTR 69262233 69262679 69262233 ID=Msa0517560-mRNA-1:three_prime_utr;Parent=Msa0517560-mRNA-1
Gene Sequence

>Msa0517560

ATGTTTGTTTTATTTATAGGACCTGCAAGGTGTTACCGTCAAATTAAGAACAAGCCATATCCAAAGTCACGTTTCTGCCGTGGTGTTCCTGATCCCAAGATCAGGATTTATGATGTTGGCATGAAGAAGAAAGGTGTTGATGAGTTTCCTTTCTGTGTTCATCTGGTCAGTTGGGAGAAGGAAAATGTGTCCAGTGAGGCACTTGAAGCTGCTAGGATTGCCTGCAACAAATACATGTCAAAGTTTGCAGGAAAGGATGCTTTCCACTTGAGAGTTAGGGTTCATCCATTCCATGTTCTTAGGATCAACAAGATGCTTTCATGTGCGGGAGCTGATAGGCTTCAGACTGGTATGAGAGGGGCGTTTGGAAAGCCACAAGGTGTTTGTGCTAGAGTGGCTATTGGGCAGGTTCTTCTTTCTGTTCGTTGCAAGGACAACAACAGCCATCATGCTCAGGAGGCTCTCCGTCGTGCTAAGTTTAAGTTCCCTGGCCGTCAAAAGATCATTGTTAGCAGGAAGTGGGGATTCACTAAGATTAACCGAGCTGATTATCTGAAGTTAAAGTCCGAGAACAGAATTATGCCTGATGGTGTGAATGCTAAGCTTCTTGGATGCCATGGACCATTGGCCAACCGTCAACCGGGAAGAGCTTTCCTGACTGCCAGCTGTAATGAGGCTTAG
Protein sequence

>Msa0517560

MFVLFIGPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKENVSSEALEAARIACNKYMSKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGVCARVAIGQVLLSVRCKDNNSHHAQEALRRAKFKFPGRQKIIVSRKWGFTKINRADYLKLKSENRIMPDGVNAKLLGCHGPLANRQPGRAFLTASCNEA*