AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1204830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204830 MtrunA17_Chr4g0053811 98.750 80 1 0 1 80 1 80 1.76e-54 163
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204830 AT2G23090.1 81.818 77 14 0 1 77 1 77 1.45e-43 135

Find 34 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCCGATAAAGAAATT+AGG 0.147345 8_1:+60701357 Msa1204830:five_prime_UTR
CATGCTTCCCTTCATGCTTC+TGG 0.193112 8_1:-60703193 None:intergenic
CAAAACCCTAATTTCTTTAT+CGG 0.268738 8_1:-60701363 None:intergenic
GATACATCAGTTTATATATT+CGG 0.294514 8_1:-60701285 None:intergenic
ATACATTATATCATATTATA+TGG 0.295545 8_1:+60703219 Msa1204830:three_prime_UTR
CAAGTGAAGAGATCAGATTT+TGG 0.307415 8_1:-60703154 None:intergenic
GTCAAAGTGTAACTGTTTAA+TGG 0.312796 8_1:+60703368 Msa1204830:three_prime_UTR
TTCTTCCGATAAAGAAATTA+GGG 0.339605 8_1:+60701358 Msa1204830:five_prime_UTR
CTCCGCAATCAACCTAAAAC+TGG 0.386217 8_1:+60703472 Msa1204830:three_prime_UTR
AAAACTGGTAAATTATTCAT+TGG 0.396392 8_1:+60703487 Msa1204830:three_prime_UTR
TTGAGGGTCTCTCTCAGCCA+TGG 0.415376 8_1:+60701383 Msa1204830:exon
TGAGGGTCTCTCTCAGCCAT+GGG 0.480356 8_1:+60701384 Msa1204830:exon
TGTGCGCAAAACGAAAATAA+TGG 0.480751 8_1:-60701325 None:intergenic
CTCGAGAAGCAGAAGAATGC+TGG 0.496068 8_1:+60701450 Msa1204830:CDS
ATCTTAAACCAGAAGCATGA+AGG 0.503983 8_1:+60703185 Msa1204830:CDS
ACTGGTAAATTATTCATTGG+TGG 0.510979 8_1:+60703490 Msa1204830:three_prime_UTR
CATGCTTCTGGTTTAAGATG+AGG 0.513870 8_1:-60703181 None:intergenic
GGTGGCTAGGGAAGGACAGA+AGG 0.515813 8_1:+60703287 Msa1204830:three_prime_UTR
CAGCATTCTTCTGCTTCTCG+AGG 0.532542 8_1:-60701449 None:intergenic
TCTCTCTCAGCCATGGGTGG+AGG 0.540024 8_1:+60701390 Msa1204830:exon
TTGACCGTTACCTCCACCCA+TGG 0.542711 8_1:-60701400 None:intergenic
TCAGCCATGGGTGGAGGTAA+CGG 0.548723 8_1:+60701396 Msa1204830:exon
GGGTCTCTCTCAGCCATGGG+TGG 0.552601 8_1:+60701387 Msa1204830:exon
TCTTAAACCAGAAGCATGAA+GGG 0.561236 8_1:+60703186 Msa1204830:exon
CTTGCACTTAACTTCTGATG+TGG 0.570945 8_1:-60703110 None:intergenic
GGTAAATTATTCATTGGTGG+TGG 0.574723 8_1:+60703493 Msa1204830:three_prime_UTR
GTGGCTAGGGAAGGACAGAA+GGG 0.578639 8_1:+60703288 Msa1204830:three_prime_UTR
AGTTTCTCACACTTCAGCAA+GGG 0.589139 8_1:-60703331 None:intergenic
CAGTTTCTCACACTTCAGCA+AGG 0.590243 8_1:-60703332 None:intergenic
AAGCAGAAGAATGCTGGCAA+AGG 0.595797 8_1:+60701456 Msa1204830:CDS
AAGCTAAGATGGCTCGCGAG+AGG 0.616608 8_1:+60701424 Msa1204830:CDS
CACATCAGAAGTTAAGTGCA+AGG 0.621035 8_1:+60703111 Msa1204830:CDS
TGGCTAGGGAAGGACAGAAG+GGG 0.672831 8_1:+60703289 Msa1204830:three_prime_UTR
TAAGTGCAAGGAGCATGCTG+AGG 0.676702 8_1:+60703123 Msa1204830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATGAAAATTAGAAATTA+GGG + chr8_1:60701504-60701523 Msa1204830:intron 10.0%
!!! TTTTTTTTTTTTTGTTTCAA+AGG + chr8_1:60701767-60701786 Msa1204830:intron 10.0%
!!! TTTTTTTTTTTTGTTTCAAA+GGG + chr8_1:60701768-60701787 Msa1204830:intron 10.0%
!!! CTATGTTTTATAAATTTTTA+AGG - chr8_1:60702466-60702485 None:intergenic 10.0%
!! TATAAAATTTATCATTACAA+CGG + chr8_1:60702971-60702990 Msa1204830:intron 10.0%
!! ATACATTATATCATATTATA+TGG + chr8_1:60703219-60703238 Msa1204830:three_prime_UTR 10.0%
!!! CTAATTTCTAATTTTCATTT+AGG - chr8_1:60701505-60701524 None:intergenic 15.0%
!! CTAAATGAAAATTAGAAATT+AGG + chr8_1:60701503-60701522 Msa1204830:intron 15.0%
!! AAACAAATTGATGAAATTAT+TGG - chr8_1:60701750-60701769 None:intergenic 15.0%
!!! AATGAAAATTTACTTTTCAA+GGG - chr8_1:60701955-60701974 None:intergenic 15.0%
!! ATGAAATTGAATAATATCAA+TGG + chr8_1:60702265-60702284 Msa1204830:intron 15.0%
!! TAATATTGTGTTAAAAATCT+TGG + chr8_1:60703401-60703420 Msa1204830:three_prime_UTR 15.0%
!! GATACATCAGTTTATATATT+CGG - chr8_1:60701288-60701307 None:intergenic 20.0%
!!! TGATTTTTTCGTTGAAAAAT+TGG + chr8_1:60701529-60701548 Msa1204830:intron 20.0%
!!! GATTTTTTCGTTGAAAAATT+GGG + chr8_1:60701530-60701549 Msa1204830:intron 20.0%
!!! GATTATTAACAGAAGTTTTA+AGG + chr8_1:60701913-60701932 Msa1204830:intron 20.0%
!!! CAATGAAAATTTACTTTTCA+AGG - chr8_1:60701956-60701975 None:intergenic 20.0%
!!! CTTGAAAAGTAAATTTTCAT+TGG + chr8_1:60701954-60701973 Msa1204830:intron 20.0%
!!! TTTTCATTGGTTAAACATTT+TGG + chr8_1:60701967-60701986 Msa1204830:intron 20.0%
!!! TTTCATTGGTTAAACATTTT+GGG + chr8_1:60701968-60701987 Msa1204830:intron 20.0%
!! AATATCAATGGTACTTAATT+TGG + chr8_1:60702277-60702296 Msa1204830:intron 20.0%
!! TTGAATATTAAACCCAAAAA+TGG + chr8_1:60702350-60702369 Msa1204830:intron 20.0%
!!! ACACAAAATTATCCATTTTT+GGG - chr8_1:60702365-60702384 None:intergenic 20.0%
!! CAATAATGAATACTAATGTA+AGG - chr8_1:60702509-60702528 None:intergenic 20.0%
!!! CATATTGAAGAAAACTTTTT+GGG - chr8_1:60702799-60702818 None:intergenic 20.0%
!!! ACATATTGAAGAAAACTTTT+TGG - chr8_1:60702800-60702819 None:intergenic 20.0%
!!! CATATTGAATGAAACTTTTT+GGG - chr8_1:60702900-60702919 None:intergenic 20.0%
!!! ACATATTGAATGAAACTTTT+TGG - chr8_1:60702901-60702920 None:intergenic 20.0%
!! ACAACATTATAATCAGTAAA+GGG - chr8_1:60703044-60703063 None:intergenic 20.0%
!!! ATGAATAATTTACCAGTTTT+AGG - chr8_1:60703487-60703506 None:intergenic 20.0%
!! AAAACTGGTAAATTATTCAT+TGG + chr8_1:60703487-60703506 Msa1204830:three_prime_UTR 20.0%
! TTCTTCCGATAAAGAAATTA+GGG + chr8_1:60701358-60701377 Msa1204830:five_prime_UTR 25.0%
! CAAAACCCTAATTTCTTTAT+CGG - chr8_1:60701366-60701385 None:intergenic 25.0%
!! ATAAAGAAATTAGGGTTTTG+AGG + chr8_1:60701366-60701385 Msa1204830:five_prime_UTR 25.0%
!!! TAAAGAAATTAGGGTTTTGA+GGG + chr8_1:60701367-60701386 Msa1204830:five_prime_UTR 25.0%
! TTAATTATACGTGTTAGATC+TGG + chr8_1:60701568-60701587 Msa1204830:intron 25.0%
!!! AATTTTTGTTTTGTACATGC+AGG + chr8_1:60701602-60701621 Msa1204830:intron 25.0%
!!! CTTTTTTGTTTTTTTCAAGC+TGG - chr8_1:60701631-60701650 None:intergenic 25.0%
!! TTATAGATCTGAAGTATATG+TGG + chr8_1:60701682-60701701 Msa1204830:intron 25.0%
! CAGAACTATGATTGTAAAAT+TGG + chr8_1:60701876-60701895 Msa1204830:intron 25.0%
!!! ATTAACAGAAGTTTTAAGGA+TGG + chr8_1:60701917-60701936 Msa1204830:intron 25.0%
! TACTTGAAATTCGTTATGTT+AGG + chr8_1:60702082-60702101 Msa1204830:intron 25.0%
! ACTTGAAATTCGTTATGTTA+GGG + chr8_1:60702083-60702102 Msa1204830:intron 25.0%
! GAACAAGTTAAAGAATTTGA+GGG + chr8_1:60702167-60702186 Msa1204830:intron 25.0%
!! CACACAAAATTATCCATTTT+TGG - chr8_1:60702366-60702385 None:intergenic 25.0%
!! CAAAAATGGATAATTTTGTG+TGG + chr8_1:60702364-60702383 Msa1204830:intron 25.0%
! TTAAGAAATTCTGACTTCTT+TGG + chr8_1:60702402-60702421 Msa1204830:intron 25.0%
! TTCCCAAACATATTGATATT+TGG + chr8_1:60702535-60702554 Msa1204830:intron 25.0%
! TGCCAAATATCAATATGTTT+GGG - chr8_1:60702540-60702559 None:intergenic 25.0%
! AAGTTTCATTCAATATGTCT+TGG + chr8_1:60702903-60702922 Msa1204830:intron 25.0%
! CACAACATTATAATCAGTAA+AGG - chr8_1:60703045-60703064 None:intergenic 25.0%
CTTCTTCCGATAAAGAAATT+AGG + chr8_1:60701357-60701376 Msa1204830:five_prime_UTR 30.0%
TTTCATTTAGGAATTGTCGA+GGG - chr8_1:60701493-60701512 None:intergenic 30.0%
! TTTTCATTTAGGAATTGTCG+AGG - chr8_1:60701494-60701513 None:intergenic 30.0%
AAAGCAATCTTGATTATACC+TGG - chr8_1:60701664-60701683 None:intergenic 30.0%
!!! TATTTTGGTTCCATGATGTT+TGG + chr8_1:60702006-60702025 Msa1204830:intron 30.0%
! TTGGCTTATGAGCATTTTAT+CGG + chr8_1:60702025-60702044 Msa1204830:intron 30.0%
ATAAAGGGAAAGGAATCTAA+TGG + chr8_1:60702125-60702144 Msa1204830:intron 30.0%
TAAAGGGAAAGGAATCTAAT+GGG + chr8_1:60702126-60702145 Msa1204830:intron 30.0%
CGAACAAGTTAAAGAATTTG+AGG + chr8_1:60702166-60702185 Msa1204830:intron 30.0%
GATCAAACTGCAAAAATGTA+TGG - chr8_1:60702217-60702236 None:intergenic 30.0%
CTATTGTTAAGTTTCTACAG+AGG - chr8_1:60702326-60702345 None:intergenic 30.0%
GTGCCAAATATCAATATGTT+TGG - chr8_1:60702541-60702560 None:intergenic 30.0%
ACATATTGATATTTGGCACT+TGG + chr8_1:60702542-60702561 Msa1204830:intron 30.0%
TGTAAGTTCATTACGAGTAT+TGG + chr8_1:60702644-60702663 Msa1204830:intron 30.0%
TAGTCATTGAATATGCACAT+AGG + chr8_1:60702938-60702957 Msa1204830:intron 30.0%
TTATCATTACAACGGTCTTA+AGG + chr8_1:60702979-60702998 Msa1204830:intron 30.0%
! AAGTGAAGAGATCAGATTTT+GGG - chr8_1:60703156-60703175 None:intergenic 30.0%
! GAATTCCTATGTGTTTTTCT+TGG + chr8_1:60703266-60703285 Msa1204830:three_prime_UTR 30.0%
GTCAAAGTGTAACTGTTTAA+TGG + chr8_1:60703368-60703387 Msa1204830:three_prime_UTR 30.0%
ACTGGTAAATTATTCATTGG+TGG + chr8_1:60703490-60703509 Msa1204830:three_prime_UTR 30.0%
TGTGCGCAAAACGAAAATAA+TGG - chr8_1:60701328-60701347 None:intergenic 35.0%
TAACGGTCAAAAAGCTAAGA+TGG + chr8_1:60701413-60701432 Msa1204830:CDS 35.0%
CAAAAAAGCTATGTCAATCC+AGG + chr8_1:60701643-60701662 Msa1204830:CDS 35.0%
!! ACAATCATAGTTCTGAACAG+AGG - chr8_1:60701871-60701890 None:intergenic 35.0%
AATTCGTTATGTTAGGGAGT+TGG + chr8_1:60702089-60702108 Msa1204830:intron 35.0%
ATTCGTTATGTTAGGGAGTT+GGG + chr8_1:60702090-60702109 Msa1204830:intron 35.0%
! TGGGTTGGGAGAAAATATAA+AGG + chr8_1:60702109-60702128 Msa1204830:intron 35.0%
GGGTTGGGAGAAAATATAAA+GGG + chr8_1:60702110-60702129 Msa1204830:intron 35.0%
GGGAGAAAATATAAAGGGAA+AGG + chr8_1:60702115-60702134 Msa1204830:intron 35.0%
! TAAGTTTCTACAGAGGCTTT+TGG - chr8_1:60702319-60702338 None:intergenic 35.0%
GAAATTCTGACTTCTTTGGT+TGG + chr8_1:60702406-60702425 Msa1204830:intron 35.0%
CACAGTATGTAAGTTCCTTT+CGG + chr8_1:60702595-60702614 Msa1204830:intron 35.0%
! GTTTGACAAGTTCTGTTTTG+AGG + chr8_1:60702721-60702740 Msa1204830:intron 35.0%
! TGCATTGCTGTAGTATTGAA+TGG + chr8_1:60703016-60703035 Msa1204830:intron 35.0%
! CAAGTGAAGAGATCAGATTT+TGG - chr8_1:60703157-60703176 None:intergenic 35.0%
! ATCTTAAACCAGAAGCATGA+AGG + chr8_1:60703185-60703204 Msa1204830:CDS 35.0%
! TCTTAAACCAGAAGCATGAA+GGG + chr8_1:60703186-60703205 Msa1204830:exon 35.0%
! TTCCTATGTGTTTTTCTTGG+TGG + chr8_1:60703269-60703288 Msa1204830:three_prime_UTR 35.0%
!!! TACCAGTTTTAGGTTGATTG+CGG - chr8_1:60703477-60703496 None:intergenic 35.0%
GGTAAATTATTCATTGGTGG+TGG + chr8_1:60703493-60703512 Msa1204830:three_prime_UTR 35.0%
TTTAGGAATTGTCGAGGGAA+TGG - chr8_1:60701488-60701507 None:intergenic 40.0%
GTTTCAAAGGGTTCACTGTT+TGG + chr8_1:60701780-60701799 Msa1204830:intron 40.0%
TGTTTGGATCCCCTTGATTT+GGG + chr8_1:60701796-60701815 Msa1204830:intron 40.0%
AAAACTGAGCCCCAAATCAA+GGG - chr8_1:60701809-60701828 None:intergenic 40.0%
TAAAACTGAGCCCCAAATCA+AGG - chr8_1:60701810-60701829 None:intergenic 40.0%
TGCTCATAAGCCAAACATCA+TGG - chr8_1:60702019-60702038 None:intergenic 40.0%
! GCTTATGAGCATTTTATCGG+AGG + chr8_1:60702028-60702047 Msa1204830:intron 40.0%
TTATGTTAGGGAGTTGGGTT+GGG + chr8_1:60702095-60702114 Msa1204830:intron 40.0%
! CTTCTTTGGTTGGCGTATTT+TGG + chr8_1:60702416-60702435 Msa1204830:intron 40.0%
AGCAACATACAGACTGTAGT+TGG + chr8_1:60702699-60702718 Msa1204830:intron 40.0%
GCATTATGCAGTTTACACGA+TGG - chr8_1:60702766-60702785 None:intergenic 40.0%
GTACTATGCAGTTTACACAG+TGG - chr8_1:60702870-60702889 None:intergenic 40.0%
! TGATTGATGATTGCAGTGCA+AGG + chr8_1:60703063-60703082 Msa1204830:intron 40.0%
CTTGCACTTAACTTCTGATG+TGG - chr8_1:60703113-60703132 None:intergenic 40.0%
CACATCAGAAGTTAAGTGCA+AGG + chr8_1:60703111-60703130 Msa1204830:CDS 40.0%
!! CATGCTTCTGGTTTAAGATG+AGG - chr8_1:60703184-60703203 None:intergenic 40.0%
AGCCACCAAGAAAAACACAT+AGG - chr8_1:60703274-60703293 None:intergenic 40.0%
!! ATGTGTTTTTCTTGGTGGCT+AGG + chr8_1:60703274-60703293 Msa1204830:three_prime_UTR 40.0%
!! TGTGTTTTTCTTGGTGGCTA+GGG + chr8_1:60703275-60703294 Msa1204830:three_prime_UTR 40.0%
AGTTTCTCACACTTCAGCAA+GGG - chr8_1:60703334-60703353 None:intergenic 40.0%
AAGCAGAAGAATGCTGGCAA+AGG + chr8_1:60701456-60701475 Msa1204830:CDS 45.0%
CTGTTTGGATCCCCTTGATT+TGG + chr8_1:60701795-60701814 Msa1204830:intron 45.0%
! GTTTGGATCCCCTTGATTTG+GGG + chr8_1:60701797-60701816 Msa1204830:intron 45.0%
AAACTGAGCCCCAAATCAAG+GGG - chr8_1:60701808-60701827 None:intergenic 45.0%
GTTATGTTAGGGAGTTGGGT+TGG + chr8_1:60702094-60702113 Msa1204830:intron 45.0%
TGTAAGTTCCTTTCGGTGAC+TGG + chr8_1:60702602-60702621 Msa1204830:intron 45.0%
ATAAAGAGCCAGTCACCGAA+AGG - chr8_1:60702613-60702632 None:intergenic 45.0%
!! GAGATCAGATTTTGGGTGTC+TGG - chr8_1:60703149-60703168 None:intergenic 45.0%
!! TTTTTCTTGGTGGCTAGGGA+AGG + chr8_1:60703279-60703298 Msa1204830:three_prime_UTR 45.0%
CAGTTTCTCACACTTCAGCA+AGG - chr8_1:60703335-60703354 None:intergenic 45.0%
CTCCGCAATCAACCTAAAAC+TGG + chr8_1:60703472-60703491 Msa1204830:three_prime_UTR 45.0%
!!! AAAATTATTTAATGTTATTT+TGG + chr8_1:60701991-60702010 Msa1204830:intron 5.0%
!!! TATGTTTTATAAATTTTTAA+GGG - chr8_1:60702465-60702484 None:intergenic 5.0%
CAGCATTCTTCTGCTTCTCG+AGG - chr8_1:60701452-60701471 None:intergenic 50.0%
CTCGAGAAGCAGAAGAATGC+TGG + chr8_1:60701450-60701469 Msa1204830:CDS 50.0%
! TAAGTGCAAGGAGCATGCTG+AGG + chr8_1:60703123-60703142 Msa1204830:CDS 50.0%
CATGCTTCCCTTCATGCTTC+TGG - chr8_1:60703196-60703215 None:intergenic 50.0%
TTGAGGGTCTCTCTCAGCCA+TGG + chr8_1:60701383-60701402 Msa1204830:exon 55.0%
TGAGGGTCTCTCTCAGCCAT+GGG + chr8_1:60701384-60701403 Msa1204830:exon 55.0%
TCAGCCATGGGTGGAGGTAA+CGG + chr8_1:60701396-60701415 Msa1204830:exon 55.0%
TTGACCGTTACCTCCACCCA+TGG - chr8_1:60701403-60701422 None:intergenic 55.0%
AAGCTAAGATGGCTCGCGAG+AGG + chr8_1:60701424-60701443 Msa1204830:CDS 55.0%
GTGGCTAGGGAAGGACAGAA+GGG + chr8_1:60703288-60703307 Msa1204830:three_prime_UTR 55.0%
TGGCTAGGGAAGGACAGAAG+GGG + chr8_1:60703289-60703308 Msa1204830:three_prime_UTR 55.0%
TCTCTCTCAGCCATGGGTGG+AGG + chr8_1:60701390-60701409 Msa1204830:exon 60.0%
GGTGGCTAGGGAAGGACAGA+AGG + chr8_1:60703287-60703306 Msa1204830:three_prime_UTR 60.0%
GGGTCTCTCTCAGCCATGGG+TGG + chr8_1:60701387-60701406 Msa1204830:exon 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 60701217 60703526 60701217 ID=Msa1204830;Name=Msa1204830
chr8_1 mRNA 60701217 60703526 60701217 ID=Msa1204830-mRNA-1;Parent=Msa1204830;Name=Msa1204830-mRNA-1;_AED=0.41;_eAED=0.41;_QI=185|1|1|1|1|1|3|322|80
chr8_1 exon 60701217 60701477 60701217 ID=Msa1204830-mRNA-1:exon:19718;Parent=Msa1204830-mRNA-1
chr8_1 exon 60701624 60701664 60701624 ID=Msa1204830-mRNA-1:exon:19719;Parent=Msa1204830-mRNA-1
chr8_1 exon 60703079 60703526 60703079 ID=Msa1204830-mRNA-1:exon:19720;Parent=Msa1204830-mRNA-1
chr8_1 five_prime_UTR 60701217 60701401 60701217 ID=Msa1204830-mRNA-1:five_prime_utr;Parent=Msa1204830-mRNA-1
chr8_1 CDS 60701402 60701477 60701402 ID=Msa1204830-mRNA-1:cds;Parent=Msa1204830-mRNA-1
chr8_1 CDS 60701624 60701664 60701624 ID=Msa1204830-mRNA-1:cds;Parent=Msa1204830-mRNA-1
chr8_1 CDS 60703079 60703204 60703079 ID=Msa1204830-mRNA-1:cds;Parent=Msa1204830-mRNA-1
chr8_1 three_prime_UTR 60703205 60703526 60703205 ID=Msa1204830-mRNA-1:three_prime_utr;Parent=Msa1204830-mRNA-1
Gene Sequence

>Msa1204830

ATGGGTGGAGGTAACGGTCAAAAAGCTAAGATGGCTCGCGAGAGGAACCTCGAGAAGCAGAAGAATGCTGGCAAAGGAAGCCAGCTTGAAAAAAACAAAAAAGCTATGTCAATCCAGTGCAAGGTGTGCATGCAAACATTTATCTGCACCACATCAGAAGTTAAGTGCAAGGAGCATGCTGAGGCCAGACACCCAAAATCTGATCTCTTCACTTGTTTTCCTCATCTTAAACCAGAAGCATGA

Protein sequence

>Msa1204830

MGGGNGQKAKMARERNLEKQKNAGKGSQLEKNKKAMSIQCKVCMQTFICTTSEVKCKEHAEARHPKSDLFTCFPHLKPEA*