AlfalfaGEDB | Msa1204850

M. sativa cultivar zhongmu-4 / Msa1204850

KEGG: -
GO: -
PFAM: PF04419.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1204850	sp\|O64818\|Y2309_ARATH	81.818	77	14	0	1	77	1	77	1.43e-42	135

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1204850	G7JCT6	98.750	80	1	0	1	80	1	80	9.16e-51	163

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001450	Msa1204850	0.802624	5.135486e-49	-8.615850e-47
Msa0004540	Msa1204850	0.814594	1.422818e-51	-8.615850e-47
Msa0008090	Msa1204850	0.805279	1.441226e-49	-8.615850e-47
Msa0008410	Msa1204850	0.811473	6.878919e-51	-8.615850e-47
Msa0027890	Msa1204850	0.810978	8.807044e-51	-8.615850e-47
Msa0029630	Msa1204850	0.807559	4.765011e-50	-8.615850e-47
Msa0039250	Msa1204850	0.823910	1.073662e-53	-8.615850e-47
Msa0041690	Msa1204850	0.801051	1.080447e-48	-8.615850e-47
Msa0047980	Msa1204850	0.805850	1.094034e-49	-8.615850e-47
Msa0055100	Msa1204850	0.825213	5.300017e-54	-8.615850e-47
Msa0057610	Msa1204850	0.802251	6.129112e-49	-8.615850e-47
Msa0061970	Msa1204850	0.807923	3.987690e-50	-8.615850e-47
Msa0064350	Msa1204850	0.815348	9.678163e-52	-8.615850e-47
Msa0068000	Msa1204850	0.815691	8.119672e-52	-8.615850e-47
Msa0068020	Msa1204850	0.801192	1.011011e-48	-8.615850e-47
Msa0069330	Msa1204850	0.822410	2.404670e-53	-8.615850e-47
Msa0070430	Msa1204850	0.809335	1.990697e-50	-8.615850e-47
Msa0073670	Msa1204850	0.827206	1.778092e-54	-8.615850e-47
Msa0074750	Msa1204850	0.809270	2.055518e-50	-8.615850e-47
Msa0076040	Msa1204850	0.825536	4.443478e-54	-8.615850e-47
Msa0079920	Msa1204850	0.801721	7.876236e-49	-8.615850e-47
Msa0101180	Msa1204850	0.817281	3.578074e-52	-8.615850e-47
Msa0104550	Msa1204850	0.817910	2.580400e-52	-8.615850e-47
Msa0108550	Msa1204850	0.804411	2.187942e-49	-8.615850e-47
Msa0113240	Msa1204850	0.803236	3.837963e-49	-8.615850e-47
Msa0115370	Msa1204850	0.809887	1.514812e-50	-8.615850e-47
Msa0610090	Msa1204850	0.817477	3.231265e-52	-8.615850e-47
Msa0620230	Msa1204850	0.808390	3.170964e-50	-8.615850e-47
Msa0626220	Msa1204850	0.804215	2.404533e-49	-8.615850e-47
Msa0628290	Msa1204850	0.804446	2.152263e-49	-8.615850e-47
Msa0628420	Msa1204850	0.804382	2.218566e-49	-8.615850e-47
Msa0628500	Msa1204850	0.807544	4.800338e-50	-8.615850e-47
Msa0633570	Msa1204850	0.804261	2.351026e-49	-8.615850e-47
Msa0641190	Msa1204850	0.815387	9.490538e-52	-8.615850e-47
Msa0644030	Msa1204850	0.813346	2.681591e-51	-8.615850e-47
Msa0652300	Msa1204850	0.809806	1.577507e-50	-8.615850e-47
Msa0658480	Msa1204850	0.823817	1.129281e-53	-8.615850e-47
Msa0662630	Msa1204850	0.814766	1.303514e-51	-8.615850e-47
Msa0666230	Msa1204850	0.812016	5.240930e-51	-8.615850e-47
Msa0679100	Msa1204850	0.806594	7.624177e-50	-8.615850e-47
Msa0686080	Msa1204850	0.805462	1.319654e-49	-8.615850e-47
Msa0687630	Msa1204850	0.807000	6.259889e-50	-8.615850e-47
Msa0689500	Msa1204850	0.803403	3.545448e-49	-8.615850e-47
Msa0691690	Msa1204850	0.807073	6.040660e-50	-8.615850e-47
Msa0697720	Msa1204850	0.815768	7.805765e-52	-8.615850e-47
Msa0702340	Msa1204850	0.805275	1.443854e-49	-8.615850e-47
Msa0702590	Msa1204850	0.803243	3.826461e-49	-8.615850e-47
Msa0702660	Msa1204850	0.807569	4.741603e-50	-8.615850e-47
Msa0713820	Msa1204850	0.812269	4.615245e-51	-8.615850e-47
Msa0716410	Msa1204850	0.823723	1.188172e-53	-8.615850e-47
Msa0721230	Msa1204850	0.803182	3.938306e-49	-8.615850e-47
Msa0734960	Msa1204850	0.810771	9.767095e-51	-8.615850e-47
Msa0743530	Msa1204850	0.810580	1.073954e-50	-8.615850e-47
Msa0743800	Msa1204850	0.803026	4.243192e-49	-8.615850e-47
Msa0752510	Msa1204850	0.809798	1.583422e-50	-8.615850e-47
Msa0752700	Msa1204850	0.809381	1.946031e-50	-8.615850e-47
Msa0767100	Msa1204850	0.801530	8.621335e-49	-8.615850e-47
Msa0788770	Msa1204850	0.826286	2.948311e-54	-8.615850e-47
Msa0797750	Msa1204850	0.847790	9.255478e-60	-8.615850e-47
Msa0798720	Msa1204850	0.803562	3.285771e-49	-8.615850e-47
Msa0819540	Msa1204850	0.802870	4.569688e-49	-8.615850e-47
Msa0822170	Msa1204850	0.808162	3.546364e-50	-8.615850e-47
Msa0822180	Msa1204850	0.802852	4.608171e-49	-8.615850e-47
Msa0824640	Msa1204850	0.839066	1.979124e-57	-8.615850e-47
Msa0832700	Msa1204850	0.807491	4.926031e-50	-8.615850e-47
Msa0835280	Msa1204850	0.821236	4.493829e-53	-8.615850e-47
Msa0836500	Msa1204850	0.806342	8.617723e-50	-8.615850e-47
Msa0125450	Msa1204850	0.811217	7.816604e-51	-8.615850e-47
Msa0125540	Msa1204850	0.824719	6.931395e-54	-8.615850e-47
Msa0127850	Msa1204850	0.800823	1.202747e-48	-8.615850e-47
Msa0130240	Msa1204850	0.806050	9.928254e-50	-8.615850e-47
Msa0133720	Msa1204850	0.821773	3.378063e-53	-8.615850e-47
Msa0133820	Msa1204850	0.804951	1.688013e-49	-8.615850e-47
Msa0138360	Msa1204850	0.806172	9.358564e-50	-8.615850e-47
Msa0142950	Msa1204850	0.803629	3.182859e-49	-8.615850e-47
Msa0147980	Msa1204850	0.828505	8.662410e-55	-8.615850e-47
Msa0148100	Msa1204850	0.801599	8.344926e-49	-8.615850e-47
Msa0149390	Msa1204850	0.812631	3.847798e-51	-8.615850e-47
Msa0150570	Msa1204850	0.823214	1.562630e-53	-8.615850e-47
Msa0155330	Msa1204850	0.805370	1.379536e-49	-8.615850e-47
Msa0181610	Msa1204850	0.825095	5.649523e-54	-8.615850e-47
Msa0185400	Msa1204850	0.821691	3.528727e-53	-8.615850e-47
Msa0195630	Msa1204850	0.820705	5.957263e-53	-8.615850e-47
Msa0226330	Msa1204850	0.823038	1.717522e-53	-8.615850e-47
Msa0237410	Msa1204850	0.823798	1.140715e-53	-8.615850e-47
Msa0376120	Msa1204850	0.806292	8.830974e-50	-8.615850e-47
Msa0376280	Msa1204850	0.838419	2.907934e-57	-8.615850e-47
Msa0394690	Msa1204850	0.832483	9.211111e-56	-8.615850e-47
Msa0403190	Msa1204850	0.803753	2.999522e-49	-8.615850e-47
Msa0410500	Msa1204850	0.806670	7.350420e-50	-8.615850e-47
Msa0413450	Msa1204850	0.817083	3.962482e-52	-8.615850e-47
Msa0432070	Msa1204850	0.838958	2.110060e-57	-8.615850e-47
Msa0433610	Msa1204850	0.808809	2.580015e-50	-8.615850e-47
Msa0439230	Msa1204850	0.807327	5.336686e-50	-8.615850e-47
Msa0449820	Msa1204850	0.818301	2.105689e-52	-8.615850e-47
Msa0453690	Msa1204850	0.806131	9.549024e-50	-8.615850e-47
Msa0478580	Msa1204850	0.804675	1.927893e-49	-8.615850e-47
Msa0481460	Msa1204850	0.801507	8.715659e-49	-8.615850e-47
Msa0980940	Msa1204850	0.806982	6.313888e-50	-8.615850e-47
Msa0998590	Msa1204850	0.805389	1.367017e-49	-8.615850e-47
Msa1005820	Msa1204850	0.801476	8.845472e-49	-8.615850e-47
Msa1009750	Msa1204850	0.819722	1.000506e-52	-8.615850e-47
Msa1010460	Msa1204850	0.811972	5.358140e-51	-8.615850e-47
Msa1025940	Msa1204850	0.808029	3.785869e-50	-8.615850e-47
Msa1037510	Msa1204850	0.806011	1.011896e-49	-8.615850e-47
Msa1050910	Msa1204850	0.800989	1.112287e-48	-8.615850e-47
Msa1051600	Msa1204850	0.800790	1.221403e-48	-8.615850e-47
Msa1059960	Msa1204850	0.803745	3.010377e-49	-8.615850e-47
Msa1072600	Msa1204850	0.804550	2.047159e-49	-8.615850e-47
Msa1083880	Msa1204850	0.838165	3.381257e-57	-8.615850e-47
Msa1087480	Msa1204850	0.812737	3.647117e-51	-8.615850e-47
Msa1097820	Msa1204850	0.820357	7.160458e-53	-8.615850e-47
Msa0245850	Msa1204850	0.809567	1.775264e-50	-8.615850e-47
Msa0254940	Msa1204850	0.831203	1.907048e-55	-8.615850e-47
Msa0267870	Msa1204850	0.809996	1.435631e-50	-8.615850e-47
Msa0295590	Msa1204850	0.840241	9.787428e-58	-8.615850e-47
Msa0296790	Msa1204850	0.833856	4.192890e-56	-8.615850e-47
Msa0311700	Msa1204850	0.800615	1.326098e-48	-8.615850e-47
Msa0322900	Msa1204850	0.803182	3.938306e-49	-8.615850e-47
Msa0324240	Msa1204850	0.802042	6.767138e-49	-8.615850e-47
Msa0331700	Msa1204850	0.801520	8.663608e-49	-8.615850e-47
Msa0333920	Msa1204850	0.814920	1.205084e-51	-8.615850e-47
Msa0334040	Msa1204850	0.817834	2.684239e-52	-8.615850e-47
Msa0363060	Msa1204850	0.803841	2.875700e-49	-8.615850e-47
Msa1113590	Msa1204850	0.831575	1.544730e-55	-8.615850e-47
Msa1123350	Msa1204850	0.805034	1.622322e-49	-8.615850e-47
Msa1126770	Msa1204850	0.813764	2.170198e-51	-8.615850e-47
Msa1127540	Msa1204850	0.813342	2.687174e-51	-8.615850e-47
Msa1131560	Msa1204850	0.814627	1.399310e-51	-8.615850e-47
Msa1154490	Msa1204850	0.801536	8.596915e-49	-8.615850e-47
Msa1172640	Msa1204850	0.800488	1.407570e-48	-8.615850e-47
Msa1174530	Msa1204850	0.810717	1.003020e-50	-8.615850e-47
Msa1174850	Msa1204850	0.809837	1.553096e-50	-8.615850e-47
Msa1176980	Msa1204850	0.809477	1.856125e-50	-8.615850e-47
Msa1182770	Msa1204850	0.802502	5.441176e-49	-8.615850e-47
Msa1184370	Msa1204850	0.849712	2.710962e-60	-8.615850e-47
Msa1185130	Msa1204850	0.807192	5.698295e-50	-8.615850e-47
Msa1192850	Msa1204850	0.824572	7.506514e-54	-8.615850e-47
Msa1199290	Msa1204850	0.865529	5.527460e-65	-8.615850e-47
Msa1201830	Msa1204850	0.834851	2.359999e-56	-8.615850e-47
Msa1204830	Msa1204850	0.942750	4.128260e-102	-8.615850e-47
Msa1204850	Msa1206650	0.802392	5.732596e-49	-8.615850e-47
Msa1204850	Msa1211830	0.800084	1.701344e-48	-8.615850e-47
Msa1204850	Msa1214320	0.800261	1.565564e-48	-8.615850e-47
Msa1204850	Msa1224840	0.809248	2.078651e-50	-8.615850e-47
Msa1204850	Msa1227690	0.847596	1.046600e-59	-8.615850e-47
Msa1204850	Msa1235250	0.822264	2.599493e-53	-8.615850e-47
Msa1204850	Msa1242640	0.808607	2.850647e-50	-8.615850e-47
Msa1204850	Msa1251160	0.804818	1.800073e-49	-8.615850e-47
Msa1204850	Msa1255410	0.848944	4.436466e-60	-8.615850e-47
Msa1204850	Msa1256770	0.806442	8.210629e-50	-8.615850e-47
Msa1204850	Msa1257070	0.817877	2.625226e-52	-8.615850e-47
Msa1204850	Msa1261070	0.813029	3.148115e-51	-8.615850e-47
Msa1204850	Msa1264420	0.800875	1.173898e-48	-8.615850e-47
Msa1204850	Msa1271580	0.803010	4.275603e-49	-8.615850e-47
Msa1204850	Msa1294840	0.819923	8.999870e-53	-8.615850e-47
Msa1204850	Msa1295630	0.813621	2.333593e-51	-8.615850e-47
Msa1204850	Msa1306990	0.800083	1.702348e-48	-8.615850e-47
Msa1204850	Msa1307950	0.815521	8.859418e-52	-8.615850e-47
Msa1204850	Msa1310690	0.813780	2.151845e-51	-8.615850e-47
Msa1204850	Msa1327490	0.824561	7.550231e-54	-8.615850e-47
Msa1204850	Msa1329450	0.816635	4.995496e-52	-8.615850e-47
Msa1204850	Msa1340020	0.801382	9.243288e-49	-8.615850e-47
Msa1204850	Msa1344850	0.829170	5.978240e-55	-8.615850e-47
Msa1204850	Msa1356610	0.835486	1.631801e-56	-8.615850e-47
Msa1204850	Msa1362420	0.806995	6.275270e-50	-8.615850e-47
Msa1204850	Msa1363860	0.813681	2.263184e-51	-8.615850e-47
Msa1204850	Msa1367590	0.819021	1.445177e-52	-8.615850e-47
Msa1204850	Msa1373330	0.805992	1.021197e-49	-8.615850e-47
Msa1204850	Msa1377800	0.813337	2.693576e-51	-8.615850e-47
Msa1204850	Msa1380970	0.805420	1.346377e-49	-8.615850e-47
Msa1204850	Msa1381120	0.809500	1.835386e-50	-8.615850e-47
Msa1204850	Msa1397940	0.815602	8.497421e-52	-8.615850e-47
Msa1204850	Msa1409950	0.805044	1.614271e-49	-8.615850e-47
Msa1204850	Msa1411950	0.812942	3.289278e-51	-8.615850e-47
Msa1204850	Msa1416260	0.810920	9.066334e-51	-8.615850e-47
Msa1204850	Msa1421560	0.825793	3.861447e-54	-8.615850e-47
Msa1204850	Msa1422580	0.820903	5.364038e-53	-8.615850e-47
Msa1204850	Msa1423910	0.819240	1.288394e-52	-8.615850e-47
Msa1204850	Msa1431040	0.819585	1.074922e-52	-8.615850e-47
Msa1204850	Msa1438050	0.805667	1.195037e-49	-8.615850e-47
Msa1204850	Msa1438640	0.813405	2.602588e-51	-8.615850e-47
Msa1204850	Msa1442850	0.810230	1.278334e-50	-8.615850e-47
Msa1204850	Msa1443930	0.817919	2.568596e-52	-8.615850e-47
Msa1204850	Msa1450720	0.806571	7.709925e-50	-8.615850e-47
Msa1204850	Msa1459960	0.803299	3.725235e-49	-8.615850e-47
Msa1204850	Msa1465080	0.837073	6.445509e-57	-8.615850e-47
Msa0484290	Msa1204850	0.819899	9.114590e-53	-8.615850e-47
Msa0484740	Msa1204850	0.803685	3.098627e-49	-8.615850e-47
Msa0491000	Msa1204850	0.800204	1.608641e-48	-8.615850e-47
Msa0505150	Msa1204850	0.834656	2.642277e-56	-8.615850e-47
Msa0515640	Msa1204850	0.806459	8.141383e-50	-8.615850e-47
Msa0516120	Msa1204850	0.825462	4.627636e-54	-8.615850e-47
Msa0517560	Msa1204850	0.814569	1.441482e-51	-8.615850e-47
Msa0524500	Msa1204850	0.805061	1.600815e-49	-8.615850e-47
Msa0531250	Msa1204850	0.802282	6.041925e-49	-8.615850e-47
Msa0546300	Msa1204850	0.801220	9.980238e-49	-8.615850e-47
Msa0547990	Msa1204850	0.821435	4.043014e-53	-8.615850e-47
Msa0552810	Msa1204850	0.814783	1.292424e-51	-8.615850e-47
Msa0556860	Msa1204850	0.801939	7.106089e-49	-8.615850e-47
Msa0581070	Msa1204850	0.827079	1.907332e-54	-8.615850e-47
Msa0588120	Msa1204850	0.809340	1.986396e-50	-8.615850e-47
Msa0593700	Msa1204850	0.815435	9.256867e-52	-8.615850e-47
Msa0594670	Msa1204850	0.804779	1.833955e-49	-8.615850e-47
Msa0600610	Msa1204850	0.809842	1.549547e-50	-8.615850e-47
Msa0847080	Msa1204850	0.810936	8.996417e-51	-8.615850e-47
Msa0850150	Msa1204850	0.811690	6.170811e-51	-8.615850e-47
Msa0864010	Msa1204850	0.833402	5.445072e-56	-8.615850e-47
Msa0869010	Msa1204850	0.831614	1.510483e-55	-8.615850e-47
Msa0869100	Msa1204850	0.825690	4.085544e-54	-8.615850e-47
Msa0880450	Msa1204850	0.810173	1.314567e-50	-8.615850e-47
Msa0888940	Msa1204850	0.802299	5.991633e-49	-8.615850e-47
Msa0898660	Msa1204850	0.821119	4.782045e-53	-8.615850e-47
Msa0898760	Msa1204850	0.848801	4.862265e-60	-8.615850e-47
Msa0928120	Msa1204850	0.822619	2.149690e-53	-8.615850e-47
Msa0943170	Msa1204850	0.840346	9.189245e-58	-8.615850e-47
Msa0960830	Msa1204850	0.810209	1.291755e-50	-8.615850e-47
Msa0963020	Msa1204850	0.813021	3.160745e-51	-8.615850e-47
Msa0967370	Msa1204850	0.807386	5.184064e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1204850	MtrunA17_Chr4g0053811	98.750	80	1	0	1	80	1	80	1.76e-54	163

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1204850	AT2G23090.1	81.818	77	14	0	1	77	1	77	1.45e-43	135

Find 35 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCTTCCGATAAAGAAATT+AGG	0.147345	8_1:+60712544	Msa1204850:five_prime_UTR
CATGCTTCCCTTCATGCTTC+TGG	0.193112	8_1:-60714403	None:intergenic
CAAAACCCTAATTTCTTTAT+CGG	0.268738	8_1:-60712550	None:intergenic
GATACATCAATTTATATATT+CGG	0.281395	8_1:-60712472	None:intergenic
ATACATTATATCATATTATA+TGG	0.295545	8_1:+60714429	Msa1204850:three_prime_UTR
CAAGTGAAGAGATCAGATTT+TGG	0.307415	8_1:-60714364	None:intergenic
GTCAAAGTGTAACTGTTTAA+TGG	0.312796	8_1:+60714575	Msa1204850:three_prime_UTR
TTCTTCCGATAAAGAAATTA+GGG	0.339605	8_1:+60712545	Msa1204850:five_prime_UTR
CTCCGCAATCAACCTAAAAC+TGG	0.386217	8_1:+60714679	Msa1204850:three_prime_UTR
AAAACTGGTAAATTATTCAT+TGG	0.396392	8_1:+60714694	Msa1204850:three_prime_UTR
TTGAGGGTCTCTCTCAGCCA+TGG	0.415376	8_1:+60712570	Msa1204850:exon
TGAGGGTCTCTCTCAGCCAT+GGG	0.480356	8_1:+60712571	Msa1204850:exon
TGTGCGCAAAACGAAAATAA+TGG	0.480751	8_1:-60712512	None:intergenic
CTCGAGAAGCAGAAGAATGC+TGG	0.496068	8_1:+60712637	Msa1204850:CDS
ATCTTAAACCAGAAGCATGA+AGG	0.503983	8_1:+60714395	Msa1204850:CDS
ACTGGTAAATTATTCATTGG+TGG	0.510979	8_1:+60714697	Msa1204850:three_prime_UTR
CATGCTTCTGGTTTAAGATG+AGG	0.513870	8_1:-60714391	None:intergenic
GGTGGCTAGGGAAGGACAGA+AGG	0.515813	8_1:+60714497	Msa1204850:three_prime_UTR
CAGCATTCTTCTGCTTCTCG+AGG	0.532542	8_1:-60712636	None:intergenic
TCTCTCTCAGCCATGGGTGG+AGG	0.540024	8_1:+60712577	Msa1204850:exon
CTTGCACTTCACTTCTGATG+TGG	0.540448	8_1:-60714320	None:intergenic
TTGACCGTTACCTCCACCCA+TGG	0.542711	8_1:-60712587	None:intergenic
TCAGCCATGGGTGGAGGTAA+CGG	0.548723	8_1:+60712583	Msa1204850:exon
GGGTCTCTCTCAGCCATGGG+TGG	0.552601	8_1:+60712574	Msa1204850:exon
ACAGTTTCTCACACTCAGCA+AGG	0.559837	8_1:-60714540	None:intergenic
TCTTAAACCAGAAGCATGAA+GGG	0.561236	8_1:+60714396	Msa1204850:exon
GGTAAATTATTCATTGGTGG+TGG	0.574723	8_1:+60714700	Msa1204850:three_prime_UTR
GTGGCTAGGGAAGGACAGAA+GGG	0.578639	8_1:+60714498	Msa1204850:three_prime_UTR
AAGCAGAAGAATGCTGGCAA+AGG	0.595797	8_1:+60712643	Msa1204850:CDS
CAGTTTCTCACACTCAGCAA+GGG	0.605684	8_1:-60714539	None:intergenic
AAGCTAAGATGGCTCGCGAG+AGG	0.616608	8_1:+60712611	Msa1204850:CDS
CACATCAGAAGTGAAGTGCA+AGG	0.659147	8_1:+60714321	Msa1204850:CDS
GAAGTGCAAGGAGCATGCTG+AGG	0.671964	8_1:+60714333	Msa1204850:CDS
TGGCTAGGGAAGGACAGAAG+GGG	0.672831	8_1:+60714499	Msa1204850:three_prime_UTR
TAGCCACCAAGAAAACACAT+AGG	0.678608	8_1:-60714482	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATGAAAATTAGAAATTA+GGG	+	chr8_1:60712700-60712719	Msa1204850:intron	10.0%
!!	AAAAAAATTGATGAAATTAT+TGG	-	chr8_1:60712944-60712963	None:intergenic	10.0%
!!!	TTTTTTTTTTTTTGTTTCAA+AGG	+	chr8_1:60712965-60712984	Msa1204850:intron	10.0%
!!!	TTTTTTTTTTTTGTTTCAAA+GGG	+	chr8_1:60712966-60712985	Msa1204850:intron	10.0%
!!!	CTATGTTTTATAAATTTTTA+AGG	-	chr8_1:60713677-60713696	None:intergenic	10.0%
!!	TATAAAATTTATCATTACAA+CGG	+	chr8_1:60714181-60714200	Msa1204850:intron	10.0%
!!	ATACATTATATCATATTATA+TGG	+	chr8_1:60714429-60714448	Msa1204850:three_prime_UTR	10.0%
!!	AAAATGAAAATTAGAAATTA+GGG	+	chr8_1:60712700-60712719	Msa1204850:intron	10.0%
!!	AAAAAAATTGATGAAATTAT+TGG	-	chr8_1:60712944-60712963	None:intergenic	10.0%
!!!	TTTTTTTTTTTTTGTTTCAA+AGG	+	chr8_1:60712965-60712984	Msa1204850:intron	10.0%
!!!	TTTTTTTTTTTTGTTTCAAA+GGG	+	chr8_1:60712966-60712985	Msa1204850:intron	10.0%
!!!	CTATGTTTTATAAATTTTTA+AGG	-	chr8_1:60713677-60713696	None:intergenic	10.0%
!!	TATAAAATTTATCATTACAA+CGG	+	chr8_1:60714181-60714200	Msa1204850:intron	10.0%
!!	ATACATTATATCATATTATA+TGG	+	chr8_1:60714429-60714448	Msa1204850:three_prime_UTR	10.0%
!!	GATACATCAATTTATATATT+CGG	-	chr8_1:60712475-60712494	None:intergenic	15.0%
!!!	CTAATTTCTAATTTTCATTT+TGG	-	chr8_1:60712701-60712720	None:intergenic	15.0%
!!	CAAAATGAAAATTAGAAATT+AGG	+	chr8_1:60712699-60712718	Msa1204850:intron	15.0%
!!!	AATGAAAATTTACTTTTCAA+GGG	-	chr8_1:60713150-60713169	None:intergenic	15.0%
!!	ATGAAATTGAATAATATCAA+TGG	+	chr8_1:60713477-60713496	Msa1204850:intron	15.0%
!!	TAATATTGTGTTAAAAATCT+TGG	+	chr8_1:60714608-60714627	Msa1204850:three_prime_UTR	15.0%
!!	GATACATCAATTTATATATT+CGG	-	chr8_1:60712475-60712494	None:intergenic	15.0%
!!!	CTAATTTCTAATTTTCATTT+TGG	-	chr8_1:60712701-60712720	None:intergenic	15.0%
!!	CAAAATGAAAATTAGAAATT+AGG	+	chr8_1:60712699-60712718	Msa1204850:intron	15.0%
!!!	AATGAAAATTTACTTTTCAA+GGG	-	chr8_1:60713150-60713169	None:intergenic	15.0%
!!	ATGAAATTGAATAATATCAA+TGG	+	chr8_1:60713477-60713496	Msa1204850:intron	15.0%
!!	TAATATTGTGTTAAAAATCT+TGG	+	chr8_1:60714608-60714627	Msa1204850:three_prime_UTR	15.0%
!!!	AATTTTCATTTTGGAATTGT+CGG	-	chr8_1:60712692-60712711	None:intergenic	20.0%
!!!	TGATTTTTTCGTTGAAAAAT+TGG	+	chr8_1:60712725-60712744	Msa1204850:intron	20.0%
!!!	GATTTTTTCGTTGAAAAATT+GGG	+	chr8_1:60712726-60712745	Msa1204850:intron	20.0%
!!!	AATTTTTGTTTTGTATATGC+AGG	+	chr8_1:60712798-60712817	Msa1204850:intron	20.0%
!!!	CAATGAAAATTTACTTTTCA+AGG	-	chr8_1:60713151-60713170	None:intergenic	20.0%
!!!	CTTGAAAAGTAAATTTTCAT+TGG	+	chr8_1:60713149-60713168	Msa1204850:intron	20.0%
!!!	TTTTCATTGGTTAAACATTT+TGG	+	chr8_1:60713162-60713181	Msa1204850:intron	20.0%
!!!	TTTCATTGGTTAAACATTTT+GGG	+	chr8_1:60713163-60713182	Msa1204850:intron	20.0%
!!	TACTTGAAATTCATTATGTT+AGG	+	chr8_1:60713294-60713313	Msa1204850:intron	20.0%
!!	ACTTGAAATTCATTATGTTA+GGG	+	chr8_1:60713295-60713314	Msa1204850:intron	20.0%
!!	AATATCAATGGTACTTAATT+TGG	+	chr8_1:60713489-60713508	Msa1204850:intron	20.0%
!!	CAATAATGAATACTAATGTA+AGG	-	chr8_1:60713720-60713739	None:intergenic	20.0%
!!	TTTCCAAACATATTGATATT+TGG	+	chr8_1:60713745-60713764	Msa1204850:intron	20.0%
!!!	CATATTGAAGAAAACTTTTT+GGG	-	chr8_1:60714009-60714028	None:intergenic	20.0%
!!!	ACATATTGAAGAAAACTTTT+TGG	-	chr8_1:60714010-60714029	None:intergenic	20.0%
!!!	CATATTGAATGAAACTTTTT+GGG	-	chr8_1:60714110-60714129	None:intergenic	20.0%
!!!	ACATATTGAATGAAACTTTT+TGG	-	chr8_1:60714111-60714130	None:intergenic	20.0%
!!	ACAACATTATAATCAGTAAA+GGG	-	chr8_1:60714254-60714273	None:intergenic	20.0%
!!!	ATGAATAATTTACCAGTTTT+AGG	-	chr8_1:60714694-60714713	None:intergenic	20.0%
!!	AAAACTGGTAAATTATTCAT+TGG	+	chr8_1:60714694-60714713	Msa1204850:three_prime_UTR	20.0%
!!!	AATTTTCATTTTGGAATTGT+CGG	-	chr8_1:60712692-60712711	None:intergenic	20.0%
!!!	TGATTTTTTCGTTGAAAAAT+TGG	+	chr8_1:60712725-60712744	Msa1204850:intron	20.0%
!!!	GATTTTTTCGTTGAAAAATT+GGG	+	chr8_1:60712726-60712745	Msa1204850:intron	20.0%
!!!	AATTTTTGTTTTGTATATGC+AGG	+	chr8_1:60712798-60712817	Msa1204850:intron	20.0%
!!!	CAATGAAAATTTACTTTTCA+AGG	-	chr8_1:60713151-60713170	None:intergenic	20.0%
!!!	CTTGAAAAGTAAATTTTCAT+TGG	+	chr8_1:60713149-60713168	Msa1204850:intron	20.0%
!!!	TTTTCATTGGTTAAACATTT+TGG	+	chr8_1:60713162-60713181	Msa1204850:intron	20.0%
!!!	TTTCATTGGTTAAACATTTT+GGG	+	chr8_1:60713163-60713182	Msa1204850:intron	20.0%
!!	TACTTGAAATTCATTATGTT+AGG	+	chr8_1:60713294-60713313	Msa1204850:intron	20.0%
!!	ACTTGAAATTCATTATGTTA+GGG	+	chr8_1:60713295-60713314	Msa1204850:intron	20.0%
!!	AATATCAATGGTACTTAATT+TGG	+	chr8_1:60713489-60713508	Msa1204850:intron	20.0%
!!	CAATAATGAATACTAATGTA+AGG	-	chr8_1:60713720-60713739	None:intergenic	20.0%
!!	TTTCCAAACATATTGATATT+TGG	+	chr8_1:60713745-60713764	Msa1204850:intron	20.0%
!!!	CATATTGAAGAAAACTTTTT+GGG	-	chr8_1:60714009-60714028	None:intergenic	20.0%
!!!	ACATATTGAAGAAAACTTTT+TGG	-	chr8_1:60714010-60714029	None:intergenic	20.0%
!!!	CATATTGAATGAAACTTTTT+GGG	-	chr8_1:60714110-60714129	None:intergenic	20.0%
!!!	ACATATTGAATGAAACTTTT+TGG	-	chr8_1:60714111-60714130	None:intergenic	20.0%
!!	ACAACATTATAATCAGTAAA+GGG	-	chr8_1:60714254-60714273	None:intergenic	20.0%
!!!	ATGAATAATTTACCAGTTTT+AGG	-	chr8_1:60714694-60714713	None:intergenic	20.0%
!!	AAAACTGGTAAATTATTCAT+TGG	+	chr8_1:60714694-60714713	Msa1204850:three_prime_UTR	20.0%
!	TTCTTCCGATAAAGAAATTA+GGG	+	chr8_1:60712545-60712564	Msa1204850:five_prime_UTR	25.0%
!	CAAAACCCTAATTTCTTTAT+CGG	-	chr8_1:60712553-60712572	None:intergenic	25.0%
!!	ATAAAGAAATTAGGGTTTTG+AGG	+	chr8_1:60712553-60712572	Msa1204850:five_prime_UTR	25.0%
!!!	TAAAGAAATTAGGGTTTTGA+GGG	+	chr8_1:60712554-60712573	Msa1204850:five_prime_UTR	25.0%
!!!	ATTTTCATTTTGGAATTGTC+GGG	-	chr8_1:60712691-60712710	None:intergenic	25.0%
!	TTAATTATACGTGTTAGATC+TGG	+	chr8_1:60712764-60712783	Msa1204850:intron	25.0%
!!!	CTTTTTTGTTTTTTTCAAGC+TGG	-	chr8_1:60712827-60712846	None:intergenic	25.0%
!!	TTATAGATCTGAAGTATATG+TGG	+	chr8_1:60712878-60712897	Msa1204850:intron	25.0%
!	CAGAACTTTGATTGTAAAAT+TGG	+	chr8_1:60713074-60713093	Msa1204850:intron	25.0%
!!!	TATTTTGATTCCATGATGTT+TGG	+	chr8_1:60713201-60713220	Msa1204850:intron	25.0%
!	GAACAAGTTAAAGAATTTGA+GGG	+	chr8_1:60713379-60713398	Msa1204850:intron	25.0%
!	CTTGAATATTAAACCCAAAA+TGG	+	chr8_1:60713561-60713580	Msa1204850:intron	25.0%
!!	CACACAAAATTATCCATTTT+GGG	-	chr8_1:60713577-60713596	None:intergenic	25.0%
!	TTAAGAAATTCTGACTTCTT+TGG	+	chr8_1:60713613-60713632	Msa1204850:intron	25.0%
!	AAGTTTCATTCAATATGTCT+TGG	+	chr8_1:60714113-60714132	Msa1204850:intron	25.0%
!!	TTATCATTACAACGGTTTTA+AGG	+	chr8_1:60714189-60714208	Msa1204850:intron	25.0%
!	CACAACATTATAATCAGTAA+AGG	-	chr8_1:60714255-60714274	None:intergenic	25.0%
!	TTCTTCCGATAAAGAAATTA+GGG	+	chr8_1:60712545-60712564	Msa1204850:five_prime_UTR	25.0%
!	CAAAACCCTAATTTCTTTAT+CGG	-	chr8_1:60712553-60712572	None:intergenic	25.0%
!!	ATAAAGAAATTAGGGTTTTG+AGG	+	chr8_1:60712553-60712572	Msa1204850:five_prime_UTR	25.0%
!!!	TAAAGAAATTAGGGTTTTGA+GGG	+	chr8_1:60712554-60712573	Msa1204850:five_prime_UTR	25.0%
!!!	ATTTTCATTTTGGAATTGTC+GGG	-	chr8_1:60712691-60712710	None:intergenic	25.0%
!	TTAATTATACGTGTTAGATC+TGG	+	chr8_1:60712764-60712783	Msa1204850:intron	25.0%
!!!	CTTTTTTGTTTTTTTCAAGC+TGG	-	chr8_1:60712827-60712846	None:intergenic	25.0%
!!	TTATAGATCTGAAGTATATG+TGG	+	chr8_1:60712878-60712897	Msa1204850:intron	25.0%
!	CAGAACTTTGATTGTAAAAT+TGG	+	chr8_1:60713074-60713093	Msa1204850:intron	25.0%
!!!	TATTTTGATTCCATGATGTT+TGG	+	chr8_1:60713201-60713220	Msa1204850:intron	25.0%
!	GAACAAGTTAAAGAATTTGA+GGG	+	chr8_1:60713379-60713398	Msa1204850:intron	25.0%
!	CTTGAATATTAAACCCAAAA+TGG	+	chr8_1:60713561-60713580	Msa1204850:intron	25.0%
!!	CACACAAAATTATCCATTTT+GGG	-	chr8_1:60713577-60713596	None:intergenic	25.0%
!	TTAAGAAATTCTGACTTCTT+TGG	+	chr8_1:60713613-60713632	Msa1204850:intron	25.0%
!	AAGTTTCATTCAATATGTCT+TGG	+	chr8_1:60714113-60714132	Msa1204850:intron	25.0%
!!	TTATCATTACAACGGTTTTA+AGG	+	chr8_1:60714189-60714208	Msa1204850:intron	25.0%
!	CACAACATTATAATCAGTAA+AGG	-	chr8_1:60714255-60714274	None:intergenic	25.0%
	CTTCTTCCGATAAAGAAATT+AGG	+	chr8_1:60712544-60712563	Msa1204850:five_prime_UTR	30.0%
	CAAAAAAGCTATGTCAATTC+AGG	+	chr8_1:60712839-60712858	Msa1204850:CDS	30.0%
	AGAGATTAACAGAAGTTTCA+AGG	+	chr8_1:60713108-60713127	Msa1204850:intron	30.0%
	ATTAACAGAAGTTTCAAGGA+TGG	+	chr8_1:60713112-60713131	Msa1204850:intron	30.0%
!	TTGGCTTATGAGCATTTTAA+CGG	+	chr8_1:60713220-60713239	Msa1204850:intron	30.0%
	AATTCATTATGTTAGGGAGT+TGG	+	chr8_1:60713301-60713320	Msa1204850:intron	30.0%
	ATTCATTATGTTAGGGAGTT+GGG	+	chr8_1:60713302-60713321	Msa1204850:intron	30.0%
	ATAAAGGGAAAGGAATCTAA+TGG	+	chr8_1:60713337-60713356	Msa1204850:intron	30.0%
	TAAAGGGAAAGGAATCTAAT+GGG	+	chr8_1:60713338-60713357	Msa1204850:intron	30.0%
	CGAACAAGTTAAAGAATTTG+AGG	+	chr8_1:60713378-60713397	Msa1204850:intron	30.0%
	GATCAAACTGCAAAAATGTA+TGG	-	chr8_1:60713429-60713448	None:intergenic	30.0%
	CTATTGTTAAGTTTCTACAG+AGG	-	chr8_1:60713538-60713557	None:intergenic	30.0%
!	CCACACAAAATTATCCATTT+TGG	-	chr8_1:60713578-60713597	None:intergenic	30.0%
!	CCAAAATGGATAATTTTGTG+TGG	+	chr8_1:60713575-60713594	Msa1204850:intron	30.0%
	GTGCCAAATATCAATATGTT+TGG	-	chr8_1:60713751-60713770	None:intergenic	30.0%
	ACATATTGATATTTGGCACT+TGG	+	chr8_1:60713752-60713771	Msa1204850:intron	30.0%
	TGTAAGTTCATTACGAGTAT+TGG	+	chr8_1:60713854-60713873	Msa1204850:intron	30.0%
	TAGTCATTGAATATGCACAT+AGG	+	chr8_1:60714148-60714167	Msa1204850:intron	30.0%
!	AAGTGAAGAGATCAGATTTT+GGG	-	chr8_1:60714366-60714385	None:intergenic	30.0%
!	GAAATTCCTATGTGTTTTCT+TGG	+	chr8_1:60714476-60714495	Msa1204850:three_prime_UTR	30.0%
	GTCAAAGTGTAACTGTTTAA+TGG	+	chr8_1:60714575-60714594	Msa1204850:three_prime_UTR	30.0%
	ACTGGTAAATTATTCATTGG+TGG	+	chr8_1:60714697-60714716	Msa1204850:three_prime_UTR	30.0%
	CTTCTTCCGATAAAGAAATT+AGG	+	chr8_1:60712544-60712563	Msa1204850:five_prime_UTR	30.0%
	CAAAAAAGCTATGTCAATTC+AGG	+	chr8_1:60712839-60712858	Msa1204850:CDS	30.0%
	AGAGATTAACAGAAGTTTCA+AGG	+	chr8_1:60713108-60713127	Msa1204850:intron	30.0%
	ATTAACAGAAGTTTCAAGGA+TGG	+	chr8_1:60713112-60713131	Msa1204850:intron	30.0%
!	TTGGCTTATGAGCATTTTAA+CGG	+	chr8_1:60713220-60713239	Msa1204850:intron	30.0%
	AATTCATTATGTTAGGGAGT+TGG	+	chr8_1:60713301-60713320	Msa1204850:intron	30.0%
	ATTCATTATGTTAGGGAGTT+GGG	+	chr8_1:60713302-60713321	Msa1204850:intron	30.0%
	ATAAAGGGAAAGGAATCTAA+TGG	+	chr8_1:60713337-60713356	Msa1204850:intron	30.0%
	TAAAGGGAAAGGAATCTAAT+GGG	+	chr8_1:60713338-60713357	Msa1204850:intron	30.0%
	CGAACAAGTTAAAGAATTTG+AGG	+	chr8_1:60713378-60713397	Msa1204850:intron	30.0%
	GATCAAACTGCAAAAATGTA+TGG	-	chr8_1:60713429-60713448	None:intergenic	30.0%
	CTATTGTTAAGTTTCTACAG+AGG	-	chr8_1:60713538-60713557	None:intergenic	30.0%
!	CCACACAAAATTATCCATTT+TGG	-	chr8_1:60713578-60713597	None:intergenic	30.0%
!	CCAAAATGGATAATTTTGTG+TGG	+	chr8_1:60713575-60713594	Msa1204850:intron	30.0%
	GTGCCAAATATCAATATGTT+TGG	-	chr8_1:60713751-60713770	None:intergenic	30.0%
	ACATATTGATATTTGGCACT+TGG	+	chr8_1:60713752-60713771	Msa1204850:intron	30.0%
	TGTAAGTTCATTACGAGTAT+TGG	+	chr8_1:60713854-60713873	Msa1204850:intron	30.0%
	TAGTCATTGAATATGCACAT+AGG	+	chr8_1:60714148-60714167	Msa1204850:intron	30.0%
!	AAGTGAAGAGATCAGATTTT+GGG	-	chr8_1:60714366-60714385	None:intergenic	30.0%
!	GAAATTCCTATGTGTTTTCT+TGG	+	chr8_1:60714476-60714495	Msa1204850:three_prime_UTR	30.0%
	GTCAAAGTGTAACTGTTTAA+TGG	+	chr8_1:60714575-60714594	Msa1204850:three_prime_UTR	30.0%
	ACTGGTAAATTATTCATTGG+TGG	+	chr8_1:60714697-60714716	Msa1204850:three_prime_UTR	30.0%
	TGTGCGCAAAACGAAAATAA+TGG	-	chr8_1:60712515-60712534	None:intergenic	35.0%
	TAACGGTCAAAAAGCTAAGA+TGG	+	chr8_1:60712600-60712619	Msa1204850:CDS	35.0%
	TGTTTGGATCCCCTTAATTT+GGG	+	chr8_1:60712994-60713013	Msa1204850:intron	35.0%
	AAAACTGAGCCCCAAATTAA+GGG	-	chr8_1:60713007-60713026	None:intergenic	35.0%
	TAAAACTGAGCCCCAAATTA+AGG	-	chr8_1:60713008-60713027	None:intergenic	35.0%
!!	ACAATCAAAGTTCTGAACAG+AGG	-	chr8_1:60713069-60713088	None:intergenic	35.0%
!	TGGGTTGGGAGAAAATATAA+AGG	+	chr8_1:60713321-60713340	Msa1204850:intron	35.0%
	GGGTTGGGAGAAAATATAAA+GGG	+	chr8_1:60713322-60713341	Msa1204850:intron	35.0%
	GGGAGAAAATATAAAGGGAA+AGG	+	chr8_1:60713327-60713346	Msa1204850:intron	35.0%
!	TAAGTTTCTACAGAGGCTTT+TGG	-	chr8_1:60713531-60713550	None:intergenic	35.0%
	GAAATTCTGACTTCTTTGGT+TGG	+	chr8_1:60713617-60713636	Msa1204850:intron	35.0%
	CACAGTATGTAAGTTCCTTT+CGG	+	chr8_1:60713805-60713824	Msa1204850:intron	35.0%
!	GTTTGACAAGTTCTGTTTTG+AGG	+	chr8_1:60713931-60713950	Msa1204850:intron	35.0%
!	TGCATTGCTGTAGTATTGAA+TGG	+	chr8_1:60714226-60714245	Msa1204850:intron	35.0%
!	CAAGTGAAGAGATCAGATTT+TGG	-	chr8_1:60714367-60714386	None:intergenic	35.0%
!	ATCTTAAACCAGAAGCATGA+AGG	+	chr8_1:60714395-60714414	Msa1204850:CDS	35.0%
!	TCTTAAACCAGAAGCATGAA+GGG	+	chr8_1:60714396-60714415	Msa1204850:exon	35.0%
!	ATTCCTATGTGTTTTCTTGG+TGG	+	chr8_1:60714479-60714498	Msa1204850:three_prime_UTR	35.0%
!!!	TACCAGTTTTAGGTTGATTG+CGG	-	chr8_1:60714684-60714703	None:intergenic	35.0%
	GGTAAATTATTCATTGGTGG+TGG	+	chr8_1:60714700-60714719	Msa1204850:three_prime_UTR	35.0%
	TGTGCGCAAAACGAAAATAA+TGG	-	chr8_1:60712515-60712534	None:intergenic	35.0%
	TAACGGTCAAAAAGCTAAGA+TGG	+	chr8_1:60712600-60712619	Msa1204850:CDS	35.0%
	TGTTTGGATCCCCTTAATTT+GGG	+	chr8_1:60712994-60713013	Msa1204850:intron	35.0%
	AAAACTGAGCCCCAAATTAA+GGG	-	chr8_1:60713007-60713026	None:intergenic	35.0%
	TAAAACTGAGCCCCAAATTA+AGG	-	chr8_1:60713008-60713027	None:intergenic	35.0%
!!	ACAATCAAAGTTCTGAACAG+AGG	-	chr8_1:60713069-60713088	None:intergenic	35.0%
!	TGGGTTGGGAGAAAATATAA+AGG	+	chr8_1:60713321-60713340	Msa1204850:intron	35.0%
	GGGTTGGGAGAAAATATAAA+GGG	+	chr8_1:60713322-60713341	Msa1204850:intron	35.0%
	GGGAGAAAATATAAAGGGAA+AGG	+	chr8_1:60713327-60713346	Msa1204850:intron	35.0%
!	TAAGTTTCTACAGAGGCTTT+TGG	-	chr8_1:60713531-60713550	None:intergenic	35.0%
	GAAATTCTGACTTCTTTGGT+TGG	+	chr8_1:60713617-60713636	Msa1204850:intron	35.0%
	CACAGTATGTAAGTTCCTTT+CGG	+	chr8_1:60713805-60713824	Msa1204850:intron	35.0%
!	GTTTGACAAGTTCTGTTTTG+AGG	+	chr8_1:60713931-60713950	Msa1204850:intron	35.0%
!	TGCATTGCTGTAGTATTGAA+TGG	+	chr8_1:60714226-60714245	Msa1204850:intron	35.0%
!	CAAGTGAAGAGATCAGATTT+TGG	-	chr8_1:60714367-60714386	None:intergenic	35.0%
!	ATCTTAAACCAGAAGCATGA+AGG	+	chr8_1:60714395-60714414	Msa1204850:CDS	35.0%
!	TCTTAAACCAGAAGCATGAA+GGG	+	chr8_1:60714396-60714415	Msa1204850:exon	35.0%
!	ATTCCTATGTGTTTTCTTGG+TGG	+	chr8_1:60714479-60714498	Msa1204850:three_prime_UTR	35.0%
!!!	TACCAGTTTTAGGTTGATTG+CGG	-	chr8_1:60714684-60714703	None:intergenic	35.0%
	GGTAAATTATTCATTGGTGG+TGG	+	chr8_1:60714700-60714719	Msa1204850:three_prime_UTR	35.0%
	GTTTCAAAGGGTTCTCTGTT+TGG	+	chr8_1:60712978-60712997	Msa1204850:intron	40.0%
	CTGTTTGGATCCCCTTAATT+TGG	+	chr8_1:60712993-60713012	Msa1204850:intron	40.0%
!	GTTTGGATCCCCTTAATTTG+GGG	+	chr8_1:60712995-60713014	Msa1204850:intron	40.0%
	AAACTGAGCCCCAAATTAAG+GGG	-	chr8_1:60713006-60713025	None:intergenic	40.0%
	TGCTCATAAGCCAAACATCA+TGG	-	chr8_1:60713214-60713233	None:intergenic	40.0%
!!	GCTTATGAGCATTTTAACGG+AGG	+	chr8_1:60713223-60713242	Msa1204850:intron	40.0%
	ATTATGTTAGGGAGTTGGGT+TGG	+	chr8_1:60713306-60713325	Msa1204850:intron	40.0%
	TTATGTTAGGGAGTTGGGTT+GGG	+	chr8_1:60713307-60713326	Msa1204850:intron	40.0%
!	CTTCTTTGGTTGGCGTATTT+TGG	+	chr8_1:60713627-60713646	Msa1204850:intron	40.0%
	AGCAACATACAGACTGTAGT+TGG	+	chr8_1:60713909-60713928	Msa1204850:intron	40.0%
	GCATTATGCAGTTTACACGA+TGG	-	chr8_1:60713976-60713995	None:intergenic	40.0%
	GTACTATGCAGTTTACACAG+TGG	-	chr8_1:60714080-60714099	None:intergenic	40.0%
!	TGATTGATGATTGCAGTGCA+AGG	+	chr8_1:60714273-60714292	Msa1204850:intron	40.0%
!!	CATGCTTCTGGTTTAAGATG+AGG	-	chr8_1:60714394-60714413	None:intergenic	40.0%
	TAGCCACCAAGAAAACACAT+AGG	-	chr8_1:60714485-60714504	None:intergenic	40.0%
!!	TATGTGTTTTCTTGGTGGCT+AGG	+	chr8_1:60714484-60714503	Msa1204850:three_prime_UTR	40.0%
!!	ATGTGTTTTCTTGGTGGCTA+GGG	+	chr8_1:60714485-60714504	Msa1204850:three_prime_UTR	40.0%
	GTTTCAAAGGGTTCTCTGTT+TGG	+	chr8_1:60712978-60712997	Msa1204850:intron	40.0%
	CTGTTTGGATCCCCTTAATT+TGG	+	chr8_1:60712993-60713012	Msa1204850:intron	40.0%
!	GTTTGGATCCCCTTAATTTG+GGG	+	chr8_1:60712995-60713014	Msa1204850:intron	40.0%
	AAACTGAGCCCCAAATTAAG+GGG	-	chr8_1:60713006-60713025	None:intergenic	40.0%
	TGCTCATAAGCCAAACATCA+TGG	-	chr8_1:60713214-60713233	None:intergenic	40.0%
!!	GCTTATGAGCATTTTAACGG+AGG	+	chr8_1:60713223-60713242	Msa1204850:intron	40.0%
	ATTATGTTAGGGAGTTGGGT+TGG	+	chr8_1:60713306-60713325	Msa1204850:intron	40.0%
	TTATGTTAGGGAGTTGGGTT+GGG	+	chr8_1:60713307-60713326	Msa1204850:intron	40.0%
!	CTTCTTTGGTTGGCGTATTT+TGG	+	chr8_1:60713627-60713646	Msa1204850:intron	40.0%
	AGCAACATACAGACTGTAGT+TGG	+	chr8_1:60713909-60713928	Msa1204850:intron	40.0%
	GCATTATGCAGTTTACACGA+TGG	-	chr8_1:60713976-60713995	None:intergenic	40.0%
	GTACTATGCAGTTTACACAG+TGG	-	chr8_1:60714080-60714099	None:intergenic	40.0%
!	TGATTGATGATTGCAGTGCA+AGG	+	chr8_1:60714273-60714292	Msa1204850:intron	40.0%
!!	CATGCTTCTGGTTTAAGATG+AGG	-	chr8_1:60714394-60714413	None:intergenic	40.0%
	TAGCCACCAAGAAAACACAT+AGG	-	chr8_1:60714485-60714504	None:intergenic	40.0%
!!	TATGTGTTTTCTTGGTGGCT+AGG	+	chr8_1:60714484-60714503	Msa1204850:three_prime_UTR	40.0%
!!	ATGTGTTTTCTTGGTGGCTA+GGG	+	chr8_1:60714485-60714504	Msa1204850:three_prime_UTR	40.0%
	AAGCAGAAGAATGCTGGCAA+AGG	+	chr8_1:60712643-60712662	Msa1204850:CDS	45.0%
	TGTAAGTTCCTTTCGGTGAC+TGG	+	chr8_1:60713812-60713831	Msa1204850:intron	45.0%
	ATAAAGAGCCAGTCACCGAA+AGG	-	chr8_1:60713823-60713842	None:intergenic	45.0%
	CTTGCACTTCACTTCTGATG+TGG	-	chr8_1:60714323-60714342	None:intergenic	45.0%
	CACATCAGAAGTGAAGTGCA+AGG	+	chr8_1:60714321-60714340	Msa1204850:CDS	45.0%
!!	GAGATCAGATTTTGGGTGTC+TGG	-	chr8_1:60714359-60714378	None:intergenic	45.0%
	CAGTTTCTCACACTCAGCAA+GGG	-	chr8_1:60714542-60714561	None:intergenic	45.0%
	ACAGTTTCTCACACTCAGCA+AGG	-	chr8_1:60714543-60714562	None:intergenic	45.0%
	CTCCGCAATCAACCTAAAAC+TGG	+	chr8_1:60714679-60714698	Msa1204850:three_prime_UTR	45.0%
	AAGCAGAAGAATGCTGGCAA+AGG	+	chr8_1:60712643-60712662	Msa1204850:CDS	45.0%
	TGTAAGTTCCTTTCGGTGAC+TGG	+	chr8_1:60713812-60713831	Msa1204850:intron	45.0%
	ATAAAGAGCCAGTCACCGAA+AGG	-	chr8_1:60713823-60713842	None:intergenic	45.0%
	CTTGCACTTCACTTCTGATG+TGG	-	chr8_1:60714323-60714342	None:intergenic	45.0%
	CACATCAGAAGTGAAGTGCA+AGG	+	chr8_1:60714321-60714340	Msa1204850:CDS	45.0%
!!	GAGATCAGATTTTGGGTGTC+TGG	-	chr8_1:60714359-60714378	None:intergenic	45.0%
	CAGTTTCTCACACTCAGCAA+GGG	-	chr8_1:60714542-60714561	None:intergenic	45.0%
	ACAGTTTCTCACACTCAGCA+AGG	-	chr8_1:60714543-60714562	None:intergenic	45.0%
	CTCCGCAATCAACCTAAAAC+TGG	+	chr8_1:60714679-60714698	Msa1204850:three_prime_UTR	45.0%
!!!	TATGTTTTATAAATTTTTAA+GGG	-	chr8_1:60713676-60713695	None:intergenic	5.0%
!!!	TATGTTTTATAAATTTTTAA+GGG	-	chr8_1:60713676-60713695	None:intergenic	5.0%
	CAGCATTCTTCTGCTTCTCG+AGG	-	chr8_1:60712639-60712658	None:intergenic	50.0%
	CTCGAGAAGCAGAAGAATGC+TGG	+	chr8_1:60712637-60712656	Msa1204850:CDS	50.0%
	TGTCGGGAACAGAGAGAGAA+TGG	-	chr8_1:60712675-60712694	None:intergenic	50.0%
	CATGCTTCCCTTCATGCTTC+TGG	-	chr8_1:60714406-60714425	None:intergenic	50.0%
!!	GTTTTCTTGGTGGCTAGGGA+AGG	+	chr8_1:60714489-60714508	Msa1204850:three_prime_UTR	50.0%
	CAGCATTCTTCTGCTTCTCG+AGG	-	chr8_1:60712639-60712658	None:intergenic	50.0%
	CTCGAGAAGCAGAAGAATGC+TGG	+	chr8_1:60712637-60712656	Msa1204850:CDS	50.0%
	TGTCGGGAACAGAGAGAGAA+TGG	-	chr8_1:60712675-60712694	None:intergenic	50.0%
	CATGCTTCCCTTCATGCTTC+TGG	-	chr8_1:60714406-60714425	None:intergenic	50.0%
!!	GTTTTCTTGGTGGCTAGGGA+AGG	+	chr8_1:60714489-60714508	Msa1204850:three_prime_UTR	50.0%
	TTGAGGGTCTCTCTCAGCCA+TGG	+	chr8_1:60712570-60712589	Msa1204850:exon	55.0%
	TGAGGGTCTCTCTCAGCCAT+GGG	+	chr8_1:60712571-60712590	Msa1204850:exon	55.0%
	TCAGCCATGGGTGGAGGTAA+CGG	+	chr8_1:60712583-60712602	Msa1204850:exon	55.0%
	TTGACCGTTACCTCCACCCA+TGG	-	chr8_1:60712590-60712609	None:intergenic	55.0%
	AAGCTAAGATGGCTCGCGAG+AGG	+	chr8_1:60712611-60712630	Msa1204850:CDS	55.0%
!	GAAGTGCAAGGAGCATGCTG+AGG	+	chr8_1:60714333-60714352	Msa1204850:CDS	55.0%
	GTGGCTAGGGAAGGACAGAA+GGG	+	chr8_1:60714498-60714517	Msa1204850:three_prime_UTR	55.0%
	TGGCTAGGGAAGGACAGAAG+GGG	+	chr8_1:60714499-60714518	Msa1204850:three_prime_UTR	55.0%
	TTGAGGGTCTCTCTCAGCCA+TGG	+	chr8_1:60712570-60712589	Msa1204850:exon	55.0%
	TGAGGGTCTCTCTCAGCCAT+GGG	+	chr8_1:60712571-60712590	Msa1204850:exon	55.0%
	TCAGCCATGGGTGGAGGTAA+CGG	+	chr8_1:60712583-60712602	Msa1204850:exon	55.0%
	TTGACCGTTACCTCCACCCA+TGG	-	chr8_1:60712590-60712609	None:intergenic	55.0%
	AAGCTAAGATGGCTCGCGAG+AGG	+	chr8_1:60712611-60712630	Msa1204850:CDS	55.0%
!	GAAGTGCAAGGAGCATGCTG+AGG	+	chr8_1:60714333-60714352	Msa1204850:CDS	55.0%
	GTGGCTAGGGAAGGACAGAA+GGG	+	chr8_1:60714498-60714517	Msa1204850:three_prime_UTR	55.0%
	TGGCTAGGGAAGGACAGAAG+GGG	+	chr8_1:60714499-60714518	Msa1204850:three_prime_UTR	55.0%
	TCTCTCTCAGCCATGGGTGG+AGG	+	chr8_1:60712577-60712596	Msa1204850:exon	60.0%
	GGTGGCTAGGGAAGGACAGA+AGG	+	chr8_1:60714497-60714516	Msa1204850:three_prime_UTR	60.0%
	TCTCTCTCAGCCATGGGTGG+AGG	+	chr8_1:60712577-60712596	Msa1204850:exon	60.0%
	GGTGGCTAGGGAAGGACAGA+AGG	+	chr8_1:60714497-60714516	Msa1204850:three_prime_UTR	60.0%
	GGGTCTCTCTCAGCCATGGG+TGG	+	chr8_1:60712574-60712593	Msa1204850:exon	65.0%
	GGGTCTCTCTCAGCCATGGG+TGG	+	chr8_1:60712574-60712593	Msa1204850:exon	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	60712404	60714733	60712404	ID=Msa1204850;Name=Msa1204850
chr8_1	mRNA	60712404	60714733	60712404	ID=Msa1204850-mRNA-1;Parent=Msa1204850;Name=Msa1204850-mRNA-1;_AED=0.41;_eAED=0.41;_QI=185\|1\|1\|1\|1\|1\|3\|319\|80
chr8_1	exon	60712404	60712664	60712404	ID=Msa1204850-mRNA-1:exon:19721;Parent=Msa1204850-mRNA-1
chr8_1	exon	60712820	60712860	60712820	ID=Msa1204850-mRNA-1:exon:19722;Parent=Msa1204850-mRNA-1
chr8_1	exon	60714289	60714733	60714289	ID=Msa1204850-mRNA-1:exon:19723;Parent=Msa1204850-mRNA-1
chr8_1	five_prime_UTR	60712404	60712588	60712404	ID=Msa1204850-mRNA-1:five_prime_utr;Parent=Msa1204850-mRNA-1
chr8_1	CDS	60712589	60712664	60712589	ID=Msa1204850-mRNA-1:cds;Parent=Msa1204850-mRNA-1
chr8_1	CDS	60712820	60712860	60712820	ID=Msa1204850-mRNA-1:cds;Parent=Msa1204850-mRNA-1
chr8_1	CDS	60714289	60714414	60714289	ID=Msa1204850-mRNA-1:cds;Parent=Msa1204850-mRNA-1
chr8_1	three_prime_UTR	60714415	60714733	60714415	ID=Msa1204850-mRNA-1:three_prime_utr;Parent=Msa1204850-mRNA-1

Gene Sequence	>Msa1204850 ATGGGTGGAGGTAACGGTCAAAAAGCTAAGATGGCTCGCGAGAGGAACCTCGAGAAGCAGAAGAATGCTGGCAAAGGAAGCCAGCTTGAAAAAAACAAAAAAGCTATGTCAATTCAGTGCAAGGTGTGCATGCAAACATTTATCTGCACCACATCAGAAGTGAAGTGCAAGGAGCATGCTGAGGCCAGACACCCAAAATCTGATCTCTTCACTTGTTTTCCTCATCTTAAACCAGAAGCATGA
Protein sequence	>Msa1204850 MGGGNGQKAKMARERNLEKQKNAGKGSQLEKNKKAMSIQCKVCMQTFICTTSEVKCKEHAEARHPKSDLFTCFPHLKPEA*