AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0517700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517700 MtrunA17_Chr3g0129221 96.927 358 7 2 1 356 1 356 0.0 705
Msa0517700 MtrunA17_Chr8g0348991 30.712 267 155 8 4 250 20 276 1.18e-28 114
Msa0517700 MtrunA17_Chr4g0063531 30.225 311 193 12 2 294 60 364 2.04e-25 106
Msa0517700 MtrunA17_Chr2g0285851 29.592 294 178 11 9 280 52 338 7.54e-25 104
Msa0517700 MtrunA17_Chr8g0378511 28.618 304 193 11 9 294 77 374 1.40e-24 103
Msa0517700 MtrunA17_Chr3g0115401 24.348 345 182 8 4 270 38 381 1.46e-21 95.5
Msa0517700 MtrunA17_Chr2g0278921 24.058 345 182 7 6 270 40 384 2.29e-18 86.3
Msa0517700 MtrunA17_Chr8g0377241 27.426 237 152 7 4 224 46 278 4.40e-18 84.7
Msa0517700 MtrunA17_Chr3g0122681 29.954 217 137 7 5 212 31 241 5.84e-17 81.3
Msa0517700 MtrunA17_Chr2g0326541 25.723 311 195 11 6 290 17 317 6.30e-16 78.2
Msa0517700 MtrunA17_Chr7g0260051 30.102 196 116 5 7 186 2 192 4.89e-15 75.5
Msa0517700 MtrunA17_Chr3g0138981 24.221 289 181 11 6 277 14 281 1.33e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517700 AT2G21950.1 55.899 356 140 7 7 351 18 367 8.51e-139 399
Msa0517700 AT4G39550.1 38.421 380 194 12 5 353 19 389 4.38e-72 229
Msa0517700 AT4G39580.1 35.925 373 203 13 2 350 9 369 4.15e-64 208
Msa0517700 AT4G39240.1 34.358 358 202 10 13 356 37 375 2.80e-61 201
Msa0517700 AT5G49000.2 35.813 363 203 14 11 356 23 372 4.93e-57 190
Msa0517700 AT4G23580.1 36.413 368 193 14 1 340 9 363 3.84e-56 188
Msa0517700 AT4G33900.1 36.415 357 185 14 13 340 15 358 1.69e-55 186
Msa0517700 AT4G39590.1 36.605 377 193 17 10 355 38 399 2.30e-55 186
Msa0517700 AT5G48990.1 34.204 383 205 14 2 356 9 372 1.22e-53 181
Msa0517700 AT4G11770.1 32.593 405 195 15 10 356 12 396 2.03e-51 176
Msa0517700 AT4G29370.1 31.351 370 222 12 6 356 22 378 3.03e-51 175
Msa0517700 AT1G60570.1 34.301 379 220 14 1 356 9 381 2.18e-50 173
Msa0517700 AT4G19330.1 32.320 362 205 11 13 351 34 378 7.83e-49 169
Msa0517700 AT3G43710.1 34.006 347 206 11 10 342 26 363 1.09e-48 168
Msa0517700 AT4G39600.2 33.791 364 200 14 11 350 26 372 4.80e-48 166
Msa0517700 AT4G39600.1 33.791 364 200 14 11 350 15 361 5.33e-48 166
Msa0517700 AT4G19870.2 32.011 378 209 10 15 356 35 400 1.07e-47 166
Msa0517700 AT4G19870.1 32.011 378 209 10 15 356 35 400 1.07e-47 166
Msa0517700 AT4G14905.1 31.287 342 202 10 15 342 36 358 1.56e-47 165
Msa0517700 AT4G14905.2 31.287 342 202 10 15 342 36 358 1.56e-47 165
Msa0517700 AT2G29600.1 32.787 366 217 13 11 356 59 415 4.80e-47 165
Msa0517700 AT4G39290.1 31.551 374 212 13 2 350 5 359 6.13e-47 163
Msa0517700 AT2G29810.1 33.875 369 224 12 1 356 22 383 1.19e-46 163
Msa0517700 AT1G19930.1 34.722 360 181 11 1 340 1 326 9.62e-46 159
Msa0517700 AT4G39570.1 33.511 376 209 14 10 356 32 395 5.16e-45 159
Msa0517700 AT4G34170.1 34.899 298 167 10 13 305 15 290 8.36e-45 155
Msa0517700 AT2G29830.1 31.492 362 203 14 2 333 13 359 1.01e-44 158
Msa0517700 AT5G49000.1 32.857 350 187 14 11 356 23 328 2.92e-44 155
Msa0517700 AT4G35120.1 33.243 367 221 11 10 356 27 389 3.32e-44 157
Msa0517700 AT2G29770.1 32.432 370 215 15 2 348 22 379 5.25e-44 156
Msa0517700 AT4G38940.1 32.520 369 210 13 9 356 20 370 8.24e-44 155
Msa0517700 AT1G61540.1 33.592 387 204 18 10 356 27 400 6.54e-43 154
Msa0517700 AT5G51250.1 33.422 374 216 13 10 356 1 368 1.28e-42 152
Msa0517700 AT4G39756.1 30.495 364 213 13 12 351 22 369 5.89e-42 150
Msa0517700 AT4G11750.1 31.443 388 216 12 10 356 8 386 6.01e-42 150
Msa0517700 AT2G21680.1 32.338 402 200 18 13 356 42 429 3.50e-41 149
Msa0517700 AT5G38680.1 31.868 364 193 12 6 344 12 345 8.21e-41 147
Msa0517700 AT3G27910.1 35.000 260 149 9 105 356 47 294 6.78e-40 143
Msa0517700 AT3G06570.1 31.593 364 213 13 13 349 29 383 7.93e-40 145
Msa0517700 AT1G19470.1 32.231 363 219 15 13 356 58 412 2.03e-39 144
Msa0517700 AT2G22030.1 32.361 377 216 14 4 355 20 382 1.52e-38 141
Msa0517700 AT4G19865.1 29.947 374 215 12 15 356 35 393 1.78e-38 141
Msa0517700 AT3G46050.1 34.060 367 189 15 2 341 5 345 2.56e-38 140
Msa0517700 AT4G39760.1 29.973 367 219 14 12 356 19 369 5.46e-38 140
Msa0517700 AT1G19460.1 30.874 366 224 13 13 356 58 416 7.02e-38 140
Msa0517700 AT5G48980.1 32.021 381 207 18 11 355 33 397 2.70e-37 139
Msa0517700 AT2G29780.1 31.135 379 215 13 5 356 39 398 3.61e-37 138
Msa0517700 AT5G39560.1 32.533 375 196 16 12 344 31 390 4.80e-37 138
Msa0517700 AT4G39560.2 39.024 246 136 7 1 235 18 260 7.91e-37 134
Msa0517700 AT4G39560.1 39.024 246 136 7 1 235 18 260 7.91e-37 134
Msa0517700 AT2G44030.1 32.231 363 197 14 12 347 21 361 9.04e-37 137
Msa0517700 AT5G02980.1 34.294 347 175 12 7 341 11 316 2.24e-36 134
Msa0517700 AT5G03000.1 33.333 345 172 13 8 341 38 335 2.37e-36 135
Msa0517700 AT2G44630.1 31.832 333 190 10 12 323 26 342 4.55e-35 132
Msa0517700 AT4G11745.1 34.483 261 146 12 107 356 36 282 4.83e-35 130
Msa0517700 AT4G19250.1 34.454 238 137 8 117 351 2 223 1.57e-34 127
Msa0517700 AT2G29800.1 31.054 351 210 11 13 343 63 401 2.87e-34 130
Msa0517700 AT4G25710.1 30.239 377 228 10 6 356 23 390 5.60e-34 129
Msa0517700 AT2G29820.1 31.098 328 194 13 22 333 53 364 4.09e-33 127
Msa0517700 AT2G29780.2 33.955 268 150 8 2 250 36 295 7.80e-33 125
Msa0517700 AT5G28160.1 27.424 361 186 11 12 350 12 318 6.34e-30 117
Msa0517700 AT5G28180.1 27.049 366 182 14 12 350 39 346 2.73e-29 116
Msa0517700 AT3G24610.1 28.346 381 182 12 4 342 22 353 9.93e-29 115
Msa0517700 AT2G22050.2 36.170 235 115 12 12 223 33 255 1.06e-28 112
Msa0517700 AT2G03460.1 31.624 234 149 6 124 350 21 250 2.97e-28 111
Msa0517700 AT2G22050.1 36.364 231 112 12 12 219 33 251 5.03e-28 110
Msa0517700 AT2G44700.1 28.771 358 227 12 9 355 27 367 5.69e-28 112
Msa0517700 AT4G02310.1 29.248 359 221 14 3 350 6 342 4.85e-27 110
Msa0517700 AT2G29860.1 35.407 209 122 7 6 206 15 218 5.63e-27 107
Msa0517700 AT1G55270.1 27.763 371 223 13 9 352 78 430 7.72e-27 110
Msa0517700 AT3G10510.1 27.945 365 209 14 2 333 7 350 1.12e-26 109
Msa0517700 AT3G08810.1 27.957 372 194 11 2 349 15 336 1.48e-26 108
Msa0517700 AT4G39753.1 27.901 362 226 14 15 354 40 388 1.64e-26 109
Msa0517700 AT2G20380.1 26.684 386 209 16 2 356 6 348 7.03e-26 106
Msa0517700 AT2G41360.1 27.937 383 229 19 1 351 1 368 1.03e-25 106
Msa0517700 AT5G03020.1 30.058 346 198 11 15 348 22 335 1.18e-25 106
Msa0517700 AT4G25710.2 32.083 240 141 5 11 228 28 267 5.26e-23 97.4
Msa0517700 AT5G38670.1 27.424 361 187 17 1 356 1 291 2.29e-22 95.9
Msa0517700 AT3G61350.2 27.509 269 172 10 9 265 21 278 1.85e-21 94.4
Msa0517700 AT3G61350.1 27.509 269 172 10 9 265 21 278 1.85e-21 94.4
Msa0517700 AT4G36840.1 31.111 225 129 8 138 342 1 219 2.22e-21 92.0
Msa0517700 AT1G67480.3 29.710 276 170 10 9 266 39 308 9.59e-21 92.8
Msa0517700 AT1G67480.1 29.710 276 170 10 9 266 39 308 9.59e-21 92.8
Msa0517700 AT1G67480.2 29.710 276 170 10 9 266 39 308 9.59e-21 92.8
Msa0517700 AT1G30090.1 28.438 320 190 12 9 302 53 359 1.88e-20 92.0
Msa0517700 AT1G22040.1 25.843 356 181 8 9 281 43 398 1.91e-20 92.8
Msa0517700 AT5G02995.1 28.165 316 193 9 29 332 2 295 2.62e-20 90.5
Msa0517700 AT4G36840.2 30.583 206 119 7 138 323 1 202 2.58e-17 80.1
Msa0517700 AT1G27420.1 27.368 285 182 10 3 268 4 282 3.32e-17 82.0
Msa0517700 AT3G63220.3 25.806 310 200 11 2 294 4 300 1.38e-16 80.5
Msa0517700 AT3G63220.2 25.806 310 200 11 2 294 4 300 1.38e-16 80.5
Msa0517700 AT3G63220.1 26.230 305 195 11 7 294 2 293 3.20e-16 79.3
Msa0517700 AT2G24540.1 24.825 286 193 10 3 270 22 303 9.25e-16 78.2
Msa0517700 AT2G45406.1 42.683 82 46 1 109 190 2 82 2.84e-14 69.3
Msa0517700 AT5G02990.1 35.556 135 83 2 16 146 24 158 2.90e-13 67.4
Msa0517700 AT5G60570.2 24.883 213 148 6 1 210 41 244 7.35e-12 66.6
Msa0517700 AT5G60570.1 24.883 213 148 6 1 210 41 244 7.35e-12 66.6
Msa0517700 AT5G60570.3 24.883 213 148 6 1 210 41 244 7.35e-12 66.6
Msa0517700 AT1G16250.2 26.639 244 153 8 6 227 5 244 7.42e-12 66.6
Msa0517700 AT1G16250.1 26.639 244 153 8 6 227 5 244 7.42e-12 66.6
Msa0517700 AT1G31350.1 26.346 353 213 13 4 317 30 374 4.66e-11 63.9
Msa0517700 AT1G31350.2 26.346 353 213 13 4 317 66 410 6.55e-11 63.9

Find 148 sgRNAs with CRISPR-Local

Find 312 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTATTGCTATGATTATTT+AGG 0.101723 3_4:+69416616 Msa0517700:CDS
TGGGAAATTGGTTGTGGTTT+GGG 0.195904 3_4:+69416744 Msa0517700:CDS
ATCCATTTCTCACGAATTTC+TGG 0.252013 3_4:-69416445 None:intergenic
ATGGGATCAGGTTTGAGTTT+TGG 0.259611 3_4:-69415892 None:intergenic
GACCCGTGCAAGGCAGTTTA+TGG 0.265673 3_4:-69415933 None:intergenic
TGTATTGCTATGATTATTTA+GGG 0.266313 3_4:+69416617 Msa0517700:CDS
TGATTATTTAGGGAAAATTA+AGG 0.267251 3_4:+69416627 Msa0517700:CDS
GTGGGAAATTGGTTGTGGTT+TGG 0.275006 3_4:+69416743 Msa0517700:CDS
CTCTCCCACGCTCCATTATA+AGG 0.279859 3_4:-69416535 None:intergenic
CAGTTTATGGCTACGTCGTC+TGG 0.300590 3_4:-69415920 None:intergenic
GGCTGATGTTGGTGGGAAAT+TGG 0.300862 3_4:+69416732 Msa0517700:CDS
AAGGAAGAGGAGGGAGAGGG+AGG 0.303836 3_4:-69416135 None:intergenic
AATTGTGGGGTGAAGTTTGT+TGG 0.325029 3_4:+69416863 Msa0517700:CDS
CTTTCCCAGCGGTTGGTAAC+AGG 0.327535 3_4:-69416409 None:intergenic
GTTGGGAGGGAGCTTGATTT+AGG 0.327637 3_4:+69416559 Msa0517700:CDS
GGGGAAGGAAGAGGAGGGAG+AGG 0.332205 3_4:-69416139 None:intergenic
GTGGAGAAGAGAGGAGAGAA+TGG 0.338796 3_4:-69416000 None:intergenic
CTCCGACCAATTTGCTGATC+TGG 0.348732 3_4:-69416377 None:intergenic
GAGGAGAGAATGGATTGGTT+TGG 0.385212 3_4:-69415990 None:intergenic
CGCCACCCGCATGGAGGGAC+CGG 0.390576 3_4:-69416281 None:intergenic
CGGGGAGCCAGCGGTGGAAT+CGG 0.393995 3_4:-69416261 None:intergenic
GGAGGAGACGGTGGTTATTG+TGG 0.394778 3_4:-69416117 None:intergenic
GAGGAGGGAGAGGGAGGAGA+CGG 0.398264 3_4:-69416129 None:intergenic
CACCAGAAATTCGTGAGAAA+TGG 0.399575 3_4:+69416443 Msa0517700:CDS
AGGGGAAAGAAATGGAGATT+TGG 0.400105 3_4:+69416797 Msa0517700:CDS
GAGAGAGGTAGAGGATGTGT+TGG 0.414107 3_4:-69416057 None:intergenic
TTGATTGCCGATTCCACCGC+TGG 0.415692 3_4:+69416254 Msa0517700:CDS
TATTGTGGAGGGTGAAGAAT+TGG 0.417411 3_4:-69416102 None:intergenic
GAATAAGGATGGGGAATTGT+GGG 0.418586 3_4:+69416849 Msa0517700:CDS
TGAACAATGGAAGATCCTTT+AGG 0.424469 3_4:-69416907 None:intergenic
GTGGAAGGAGTTGAAAGGTT+TGG 0.425255 3_4:+69416672 Msa0517700:CDS
CGTCTGGGAGGGATGGGATC+AGG 0.425632 3_4:-69415904 None:intergenic
AATGTTCTTTCTGTTCCTAA+AGG 0.426640 3_4:+69416892 Msa0517700:CDS
GATTATTTAGGGAAAATTAA+GGG 0.427665 3_4:+69416628 Msa0517700:CDS
TTGGTTTGGAAACGAGGGTG+AGG 0.443707 3_4:-69415976 None:intergenic
GCTACGTCGTCTGGGAGGGA+TGG 0.445851 3_4:-69415911 None:intergenic
ATGGAGCGTGGGAGAGCGTT+GGG 0.446205 3_4:+69416542 Msa0517700:CDS
ATGTACCATCTCGTCATGTA+TGG 0.447118 3_4:+69416227 Msa0517700:CDS
GCGCGTGAATTCGCAGCTGC+TGG 0.448675 3_4:+69416301 Msa0517700:CDS
CGATTCCACCGCTGGCTCCC+CGG 0.454099 3_4:+69416262 Msa0517700:CDS
TAGAGGATGTGTTGGGTGGA+AGG 0.455700 3_4:-69416049 None:intergenic
CTACGTCGTCTGGGAGGGAT+GGG 0.457938 3_4:-69415910 None:intergenic
GAGGGAGAGGGAGGAGACGG+TGG 0.459896 3_4:-69416126 None:intergenic
AAGAAGAAGAAGAATAAGGA+TGG 0.461286 3_4:+69416838 Msa0517700:CDS
GGAGGGAGCTTGATTTAGGT+TGG 0.464558 3_4:+69416563 Msa0517700:CDS
GGGAAGGAAGAGGAGGGAGA+GGG 0.467938 3_4:-69416138 None:intergenic
GCGTAAGCGGAGCCGATTGT+AGG 0.471244 3_4:-69416160 None:intergenic
CGTAAGCGGAGCCGATTGTA+GGG 0.471976 3_4:-69416159 None:intergenic
AGAATAAGGATGGGGAATTG+TGG 0.475295 3_4:+69416848 Msa0517700:CDS
GTGTGCGGAGAGAGAGGTAG+AGG 0.475726 3_4:-69416066 None:intergenic
TGTTGGTGGGAAATTGGTTG+TGG 0.476458 3_4:+69416738 Msa0517700:CDS
CCTCTTCCTTCCCCTACAAT+CGG 0.477902 3_4:+69416148 Msa0517700:CDS
TCGATCCTGTTACCAACCGC+TGG 0.491929 3_4:+69416404 Msa0517700:CDS
AGAGGATGTGTTGGGTGGAA+GGG 0.494197 3_4:-69416048 None:intergenic
AGCGGTGGAATCGGCAATCA+AGG 0.496819 3_4:-69416252 None:intergenic
AAATAAGAAGGGGAAAGAAA+TGG 0.497591 3_4:+69416789 Msa0517700:CDS
GCTCCCCGGTCCCTCCATGC+GGG 0.498399 3_4:+69416276 Msa0517700:CDS
TGGTTTGGAAACGAGGGTGA+GGG 0.501416 3_4:-69415975 None:intergenic
GCCACCCGCATGGAGGGACC+GGG 0.502655 3_4:-69416280 None:intergenic
AGGGTTGTGTGGTGAGAGCG+TGG 0.503990 3_4:-69415956 None:intergenic
TCACGAATTTCTGGTGGACT+AGG 0.504253 3_4:-69416436 None:intergenic
GAAGAGAGGAGAGAATGGAT+TGG 0.505986 3_4:-69415995 None:intergenic
GTTTACCAAGGTTTCTGTGT+GGG 0.506144 3_4:+69416701 Msa0517700:CDS
AGGGACCGGGGAGCCAGCGG+TGG 0.509897 3_4:-69416267 None:intergenic
GGGAGAGCAACTGTTGTGGA+TGG 0.510495 3_4:+69416589 Msa0517700:CDS
GCTACAATGGCTGATGTTGG+TGG 0.511043 3_4:+69416724 Msa0517700:CDS
GAATTTGAATGCAAATAAGA+AGG 0.511518 3_4:+69416777 Msa0517700:CDS
CACGCTAGCGCGGTCGTAGA+CGG 0.513221 3_4:+69416469 Msa0517700:CDS
AGAGAGGTAGAGGATGTGTT+GGG 0.517370 3_4:-69416056 None:intergenic
AATTTGAATGCAAATAAGAA+GGG 0.517396 3_4:+69416778 Msa0517700:CDS
TTGTGGTTTGGGAGTGTCAA+AGG 0.519615 3_4:+69416755 Msa0517700:CDS
CATTTCTCACGAATTTCTGG+TGG 0.520076 3_4:-69416442 None:intergenic
GGTGAAGAATTGGAGAGAGG+TGG 0.521152 3_4:-69416092 None:intergenic
GTTTCTGTGTGGGGCTACAA+TGG 0.522064 3_4:+69416711 Msa0517700:CDS
CCGATTGTAGGGGAAGGAAG+AGG 0.526253 3_4:-69416148 None:intergenic
AATGGATTGGTTTGGAAACG+AGG 0.527115 3_4:-69415982 None:intergenic
CGTCATCGGTGGTTGCGTGC+CGG 0.527892 3_4:+69416345 Msa0517700:CDS
GAGGTAGAGGATGTGTTGGG+TGG 0.532415 3_4:-69416053 None:intergenic
TGAAGAAGAGTGGAGAAGAG+AGG 0.534364 3_4:-69416009 None:intergenic
GGGGCTACAATGGCTGATGT+TGG 0.535073 3_4:+69416721 Msa0517700:CDS
AGAAGAAGAAGAATAAGGAT+GGG 0.535671 3_4:+69416839 Msa0517700:CDS
TAGCCATAAACTGCCTTGCA+CGG 0.537984 3_4:+69415930 Msa0517700:CDS
AATGGAGCGTGGGAGAGCGT+TGG 0.540307 3_4:+69416541 Msa0517700:CDS
TGGAGCGTGTGCGGAGAGAG+AGG 0.541247 3_4:-69416072 None:intergenic
CAGCTGCTGGTGTTATCGAC+GGG 0.541290 3_4:+69416314 Msa0517700:CDS
TTTACCAAGGTTTCTGTGTG+GGG 0.550464 3_4:+69416702 Msa0517700:CDS
TCACGCGCCACCCGCATGGA+GGG 0.550890 3_4:-69416286 None:intergenic
AGCTTGATTTAGGTTGGCGC+GGG 0.552408 3_4:+69416569 Msa0517700:CDS
AGGAACACTTTCCCAGCGGT+TGG 0.557279 3_4:-69416416 None:intergenic
GGAGACGGTGGTTATTGTGG+AGG 0.557706 3_4:-69416114 None:intergenic
CTACAATGGCTGATGTTGGT+GGG 0.559479 3_4:+69416725 Msa0517700:CDS
TGGAGGGACCGGGGAGCCAG+CGG 0.559843 3_4:-69416270 None:intergenic
TATGGCTACGTCGTCTGGGA+GGG 0.561867 3_4:-69415915 None:intergenic
GAGCGAGCGGTGAAGAAGAG+TGG 0.563340 3_4:-69416019 None:intergenic
GAAGAAGAAGAATAAGGATG+GGG 0.563686 3_4:+69416840 Msa0517700:CDS
CGATCCTGTTACCAACCGCT+GGG 0.564306 3_4:+69416405 Msa0517700:CDS
GAGAGCGTGGGACCCGTGCA+AGG 0.565651 3_4:-69415943 None:intergenic
GGAGAGAGGTGGAGCGTGTG+CGG 0.565891 3_4:-69416081 None:intergenic
AGTTTATGGCTACGTCGTCT+GGG 0.567545 3_4:-69415919 None:intergenic
TTCCAGATCAGCAAATTGGT+CGG 0.568075 3_4:+69416375 Msa0517700:CDS
ATTGTAGGGGAAGGAAGAGG+AGG 0.569549 3_4:-69416145 None:intergenic
AGCCATAAACTGCCTTGCAC+GGG 0.572097 3_4:+69415931 Msa0517700:CDS
ATGGATTGGTTTGGAAACGA+GGG 0.575321 3_4:-69415981 None:intergenic
AGCGTGGGAGAGCGTTGGGA+GGG 0.581664 3_4:+69416546 Msa0517700:CDS
GAATTCACGCGCCACCCGCA+TGG 0.582008 3_4:-69416290 None:intergenic
CCCCGGTCCCTCCATGCGGG+TGG 0.583728 3_4:+69416279 Msa0517700:CDS
GGCTCCCCGGTCCCTCCATG+CGG 0.585977 3_4:+69416275 Msa0517700:CDS
CAGATCAGCAAATTGGTCGG+AGG 0.586808 3_4:+69416378 Msa0517700:CDS
GCAGCTGCTGGTGTTATCGA+CGG 0.586899 3_4:+69416313 Msa0517700:CDS
GAGCGTGGGAGAGCGTTGGG+AGG 0.588514 3_4:+69416545 Msa0517700:CDS
GAGGGTGAAGAATTGGAGAG+AGG 0.589538 3_4:-69416095 None:intergenic
GAGCTTGATTTAGGTTGGCG+CGG 0.591579 3_4:+69416568 Msa0517700:CDS
GGAGAGCAACTGTTGTGGAT+GGG 0.594745 3_4:+69416590 Msa0517700:CDS
CACCACAAAATCTACCTCAT+CGG 0.600911 3_4:+69416190 Msa0517700:CDS
TTGTAGGGGAAGGAAGAGGA+GGG 0.601044 3_4:-69416144 None:intergenic
AATAAGGATGGGGAATTGTG+GGG 0.601563 3_4:+69416850 Msa0517700:CDS
TTCACGCGCCACCCGCATGG+AGG 0.601818 3_4:-69416287 None:intergenic
TTATGGCTACGTCGTCTGGG+AGG 0.604755 3_4:-69415916 None:intergenic
AAACGAGGGTGAGGGTTGTG+TGG 0.611470 3_4:-69415967 None:intergenic
GTGGTGGATGACAGCGTAAG+CGG 0.611973 3_4:-69416173 None:intergenic
CCACCCGCATGGAGGGACCG+GGG 0.612009 3_4:-69416279 None:intergenic
GTAGCCCCACACAGAAACCT+TGG 0.612328 3_4:-69416706 None:intergenic
TTGATGTGAAGAAAGGGTTG+TGG 0.612357 3_4:+69416653 Msa0517700:CDS
GACGGGAAAATCTACGTCAT+CGG 0.619777 3_4:+69416331 Msa0517700:CDS
GCGGAGCCGATTGTAGGGGA+AGG 0.620894 3_4:-69416154 None:intergenic
GGGTTGTGGAAGGAGTTGAA+AGG 0.624861 3_4:+69416667 Msa0517700:CDS
TGATCCTTATAATGGAGCGT+GGG 0.625550 3_4:+69416531 Msa0517700:CDS
TTGATCCTTATAATGGAGCG+TGG 0.628287 3_4:+69416530 Msa0517700:CDS
AGGATCCATACATGACGAGA+TGG 0.628694 3_4:-69416232 None:intergenic
ATTTGAATGCAAATAAGAAG+GGG 0.632620 3_4:+69416779 Msa0517700:CDS
CACAAAATCTACCTCATCGG+TGG 0.633655 3_4:+69416193 Msa0517700:CDS
GGGAAAATCTACGTCATCGG+TGG 0.638696 3_4:+69416334 Msa0517700:CDS
GAGACGGTGGTTATTGTGGA+GGG 0.639572 3_4:-69416113 None:intergenic
GGAAGGGATGAGAGAGCGAG+CGG 0.643411 3_4:-69416032 None:intergenic
GCGCGGGAGAGCAACTGTTG+TGG 0.648340 3_4:+69416585 Msa0517700:CDS
TACGCGATGGCGGATCGCGG+TGG 0.651378 3_4:+69416499 Msa0517700:CDS
AACAGAAGAACAATGAACAA+TGG 0.652676 3_4:-69416920 None:intergenic
GTTTACGCGATGGCGGATCG+CGG 0.654239 3_4:+69416496 Msa0517700:CDS
TGTGAAGAAAGGGTTGTGGA+AGG 0.655527 3_4:+69416657 Msa0517700:CDS
AGACGGCAAAGTTTACGCGA+TGG 0.658808 3_4:+69416486 Msa0517700:CDS
GGGTTGTGTGGTGAGAGCGT+GGG 0.659765 3_4:-69415955 None:intergenic
CGGCAAAGTTTACGCGATGG+CGG 0.661397 3_4:+69416489 Msa0517700:CDS
GAAATGGATGCACGCTAGCG+CGG 0.679178 3_4:+69416459 Msa0517700:CDS
AGTTTACCAAGGTTTCTGTG+TGG 0.681635 3_4:+69416700 Msa0517700:CDS
GTTTGGATAAGAGTTTACCA+AGG 0.687199 3_4:+69416689 Msa0517700:CDS
GACTAGGAACACTTTCCCAG+CGG 0.689950 3_4:-69416420 None:intergenic
CGTTGACTGAACCACCGATG+AGG 0.690906 3_4:-69416204 None:intergenic
GTAAGCGGAGCCGATTGTAG+GGG 0.695680 3_4:-69416158 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTATTGCTATGATTATTT+AGG + chr3_4:69416616-69416635 Msa0517700:CDS 20.0%
!! TGTATTGCTATGATTATTTA+GGG + chr3_4:69416617-69416636 Msa0517700:CDS 20.0%
!! TGATTATTTAGGGAAAATTA+AGG + chr3_4:69416627-69416646 Msa0517700:CDS 20.0%
!! GATTATTTAGGGAAAATTAA+GGG + chr3_4:69416628-69416647 Msa0517700:CDS 20.0%
!! AATTTGAATGCAAATAAGAA+GGG + chr3_4:69416778-69416797 Msa0517700:CDS 20.0%
!! TTGTATTGCTATGATTATTT+AGG + chr3_4:69416616-69416635 Msa0517700:CDS 20.0%
!! TGTATTGCTATGATTATTTA+GGG + chr3_4:69416617-69416636 Msa0517700:CDS 20.0%
!! TGATTATTTAGGGAAAATTA+AGG + chr3_4:69416627-69416646 Msa0517700:CDS 20.0%
!! GATTATTTAGGGAAAATTAA+GGG + chr3_4:69416628-69416647 Msa0517700:CDS 20.0%
!! AATTTGAATGCAAATAAGAA+GGG + chr3_4:69416778-69416797 Msa0517700:CDS 20.0%
!!! GTTTTGTTTGATCCTTATAA+TGG + chr3_4:69416523-69416542 Msa0517700:CDS 25.0%
! GAATTTGAATGCAAATAAGA+AGG + chr3_4:69416777-69416796 Msa0517700:CDS 25.0%
! ATTTGAATGCAAATAAGAAG+GGG + chr3_4:69416779-69416798 Msa0517700:CDS 25.0%
!!! GTTTTGTTTGATCCTTATAA+TGG + chr3_4:69416523-69416542 Msa0517700:CDS 25.0%
! GAATTTGAATGCAAATAAGA+AGG + chr3_4:69416777-69416796 Msa0517700:CDS 25.0%
! ATTTGAATGCAAATAAGAAG+GGG + chr3_4:69416779-69416798 Msa0517700:CDS 25.0%
! ATTTTTCCAGATCAGCAAAT+TGG + chr3_4:69416371-69416390 Msa0517700:CDS 30.0%
AAATAAGAAGGGGAAAGAAA+TGG + chr3_4:69416789-69416808 Msa0517700:CDS 30.0%
TGTGAAGAAGAAGAAGAATA+AGG + chr3_4:69416834-69416853 Msa0517700:CDS 30.0%
AAGAAGAAGAAGAATAAGGA+TGG + chr3_4:69416838-69416857 Msa0517700:CDS 30.0%
AGAAGAAGAAGAATAAGGAT+GGG + chr3_4:69416839-69416858 Msa0517700:CDS 30.0%
AATGTTCTTTCTGTTCCTAA+AGG + chr3_4:69416892-69416911 Msa0517700:CDS 30.0%
AACAGAAGAACAATGAACAA+TGG - chr3_4:69416923-69416942 None:intergenic 30.0%
! ATTTTTCCAGATCAGCAAAT+TGG + chr3_4:69416371-69416390 Msa0517700:CDS 30.0%
AAATAAGAAGGGGAAAGAAA+TGG + chr3_4:69416789-69416808 Msa0517700:CDS 30.0%
TGTGAAGAAGAAGAAGAATA+AGG + chr3_4:69416834-69416853 Msa0517700:CDS 30.0%
AAGAAGAAGAAGAATAAGGA+TGG + chr3_4:69416838-69416857 Msa0517700:CDS 30.0%
AGAAGAAGAAGAATAAGGAT+GGG + chr3_4:69416839-69416858 Msa0517700:CDS 30.0%
AATGTTCTTTCTGTTCCTAA+AGG + chr3_4:69416892-69416911 Msa0517700:CDS 30.0%
AACAGAAGAACAATGAACAA+TGG - chr3_4:69416923-69416942 None:intergenic 30.0%
GCTGATCTGGAAAAATTATC+CGG - chr3_4:69416367-69416386 None:intergenic 35.0%
ATCCATTTCTCACGAATTTC+TGG - chr3_4:69416448-69416467 None:intergenic 35.0%
!! AAGGGTTTTGATGTGAAGAA+AGG + chr3_4:69416646-69416665 Msa0517700:CDS 35.0%
!! AGGGTTTTGATGTGAAGAAA+GGG + chr3_4:69416647-69416666 Msa0517700:CDS 35.0%
GTTTGGATAAGAGTTTACCA+AGG + chr3_4:69416689-69416708 Msa0517700:CDS 35.0%
GAAGAAGAAGAATAAGGATG+GGG + chr3_4:69416840-69416859 Msa0517700:CDS 35.0%
TGAACAATGGAAGATCCTTT+AGG - chr3_4:69416910-69416929 None:intergenic 35.0%
GCTGATCTGGAAAAATTATC+CGG - chr3_4:69416367-69416386 None:intergenic 35.0%
ATCCATTTCTCACGAATTTC+TGG - chr3_4:69416448-69416467 None:intergenic 35.0%
!! AAGGGTTTTGATGTGAAGAA+AGG + chr3_4:69416646-69416665 Msa0517700:CDS 35.0%
!! AGGGTTTTGATGTGAAGAAA+GGG + chr3_4:69416647-69416666 Msa0517700:CDS 35.0%
GTTTGGATAAGAGTTTACCA+AGG + chr3_4:69416689-69416708 Msa0517700:CDS 35.0%
GAAGAAGAAGAATAAGGATG+GGG + chr3_4:69416840-69416859 Msa0517700:CDS 35.0%
TGAACAATGGAAGATCCTTT+AGG - chr3_4:69416910-69416929 None:intergenic 35.0%
!! ATGGGATCAGGTTTGAGTTT+TGG - chr3_4:69415895-69415914 None:intergenic 40.0%
!! ATGGATTGGTTTGGAAACGA+GGG - chr3_4:69415984-69416003 None:intergenic 40.0%
!! AATGGATTGGTTTGGAAACG+AGG - chr3_4:69415985-69416004 None:intergenic 40.0%
TATTGTGGAGGGTGAAGAAT+TGG - chr3_4:69416105-69416124 None:intergenic 40.0%
CACCACAAAATCTACCTCAT+CGG + chr3_4:69416190-69416209 Msa0517700:CDS 40.0%
ATGTACCATCTCGTCATGTA+TGG + chr3_4:69416227-69416246 Msa0517700:CDS 40.0%
TTCCAGATCAGCAAATTGGT+CGG + chr3_4:69416375-69416394 Msa0517700:CDS 40.0%
! CATTTCTCACGAATTTCTGG+TGG - chr3_4:69416445-69416464 None:intergenic 40.0%
CACCAGAAATTCGTGAGAAA+TGG + chr3_4:69416443-69416462 Msa0517700:CDS 40.0%
! TTGATCCTTATAATGGAGCG+TGG + chr3_4:69416530-69416549 Msa0517700:CDS 40.0%
! TGATCCTTATAATGGAGCGT+GGG + chr3_4:69416531-69416550 Msa0517700:CDS 40.0%
TTGATGTGAAGAAAGGGTTG+TGG + chr3_4:69416653-69416672 Msa0517700:CDS 40.0%
AGTTTACCAAGGTTTCTGTG+TGG + chr3_4:69416700-69416719 Msa0517700:CDS 40.0%
GTTTACCAAGGTTTCTGTGT+GGG + chr3_4:69416701-69416720 Msa0517700:CDS 40.0%
TTTACCAAGGTTTCTGTGTG+GGG + chr3_4:69416702-69416721 Msa0517700:CDS 40.0%
TGGGAAATTGGTTGTGGTTT+GGG + chr3_4:69416744-69416763 Msa0517700:CDS 40.0%
AGGGGAAAGAAATGGAGATT+TGG + chr3_4:69416797-69416816 Msa0517700:CDS 40.0%
AGAATAAGGATGGGGAATTG+TGG + chr3_4:69416848-69416867 Msa0517700:CDS 40.0%
GAATAAGGATGGGGAATTGT+GGG + chr3_4:69416849-69416868 Msa0517700:CDS 40.0%
AATAAGGATGGGGAATTGTG+GGG + chr3_4:69416850-69416869 Msa0517700:CDS 40.0%
AATTGTGGGGTGAAGTTTGT+TGG + chr3_4:69416863-69416882 Msa0517700:CDS 40.0%
!! ATGGGATCAGGTTTGAGTTT+TGG - chr3_4:69415895-69415914 None:intergenic 40.0%
!! ATGGATTGGTTTGGAAACGA+GGG - chr3_4:69415984-69416003 None:intergenic 40.0%
!! AATGGATTGGTTTGGAAACG+AGG - chr3_4:69415985-69416004 None:intergenic 40.0%
TATTGTGGAGGGTGAAGAAT+TGG - chr3_4:69416105-69416124 None:intergenic 40.0%
CACCACAAAATCTACCTCAT+CGG + chr3_4:69416190-69416209 Msa0517700:CDS 40.0%
ATGTACCATCTCGTCATGTA+TGG + chr3_4:69416227-69416246 Msa0517700:CDS 40.0%
TTCCAGATCAGCAAATTGGT+CGG + chr3_4:69416375-69416394 Msa0517700:CDS 40.0%
! CATTTCTCACGAATTTCTGG+TGG - chr3_4:69416445-69416464 None:intergenic 40.0%
CACCAGAAATTCGTGAGAAA+TGG + chr3_4:69416443-69416462 Msa0517700:CDS 40.0%
! TTGATCCTTATAATGGAGCG+TGG + chr3_4:69416530-69416549 Msa0517700:CDS 40.0%
! TGATCCTTATAATGGAGCGT+GGG + chr3_4:69416531-69416550 Msa0517700:CDS 40.0%
TTGATGTGAAGAAAGGGTTG+TGG + chr3_4:69416653-69416672 Msa0517700:CDS 40.0%
AGTTTACCAAGGTTTCTGTG+TGG + chr3_4:69416700-69416719 Msa0517700:CDS 40.0%
GTTTACCAAGGTTTCTGTGT+GGG + chr3_4:69416701-69416720 Msa0517700:CDS 40.0%
TTTACCAAGGTTTCTGTGTG+GGG + chr3_4:69416702-69416721 Msa0517700:CDS 40.0%
TGGGAAATTGGTTGTGGTTT+GGG + chr3_4:69416744-69416763 Msa0517700:CDS 40.0%
AGGGGAAAGAAATGGAGATT+TGG + chr3_4:69416797-69416816 Msa0517700:CDS 40.0%
AGAATAAGGATGGGGAATTG+TGG + chr3_4:69416848-69416867 Msa0517700:CDS 40.0%
GAATAAGGATGGGGAATTGT+GGG + chr3_4:69416849-69416868 Msa0517700:CDS 40.0%
AATAAGGATGGGGAATTGTG+GGG + chr3_4:69416850-69416869 Msa0517700:CDS 40.0%
AATTGTGGGGTGAAGTTTGT+TGG + chr3_4:69416863-69416882 Msa0517700:CDS 40.0%
AGTTTATGGCTACGTCGTCT+GGG - chr3_4:69415922-69415941 None:intergenic 45.0%
TAGCCATAAACTGCCTTGCA+CGG + chr3_4:69415930-69415949 Msa0517700:CDS 45.0%
!! GAGGAGAGAATGGATTGGTT+TGG - chr3_4:69415993-69416012 None:intergenic 45.0%
GAAGAGAGGAGAGAATGGAT+TGG - chr3_4:69415998-69416017 None:intergenic 45.0%
TGAAGAAGAGTGGAGAAGAG+AGG - chr3_4:69416012-69416031 None:intergenic 45.0%
AGAGAGGTAGAGGATGTGTT+GGG - chr3_4:69416059-69416078 None:intergenic 45.0%
! CGATGAGGTAGATTTTGTGG+TGG - chr3_4:69416192-69416211 None:intergenic 45.0%
! CACCGATGAGGTAGATTTTG+TGG - chr3_4:69416195-69416214 None:intergenic 45.0%
CACAAAATCTACCTCATCGG+TGG + chr3_4:69416193-69416212 Msa0517700:CDS 45.0%
AGGATCCATACATGACGAGA+TGG - chr3_4:69416235-69416254 None:intergenic 45.0%
GACGGGAAAATCTACGTCAT+CGG + chr3_4:69416331-69416350 Msa0517700:CDS 45.0%
!! TCACGAATTTCTGGTGGACT+AGG - chr3_4:69416439-69416458 None:intergenic 45.0%
TGTGAAGAAAGGGTTGTGGA+AGG + chr3_4:69416657-69416676 Msa0517700:CDS 45.0%
GTGGAAGGAGTTGAAAGGTT+TGG + chr3_4:69416672-69416691 Msa0517700:CDS 45.0%
CTACAATGGCTGATGTTGGT+GGG + chr3_4:69416725-69416744 Msa0517700:CDS 45.0%
! TGTTGGTGGGAAATTGGTTG+TGG + chr3_4:69416738-69416757 Msa0517700:CDS 45.0%
GTGGGAAATTGGTTGTGGTT+TGG + chr3_4:69416743-69416762 Msa0517700:CDS 45.0%
!! TTGTGGTTTGGGAGTGTCAA+AGG + chr3_4:69416755-69416774 Msa0517700:CDS 45.0%
AGTTTATGGCTACGTCGTCT+GGG - chr3_4:69415922-69415941 None:intergenic 45.0%
TAGCCATAAACTGCCTTGCA+CGG + chr3_4:69415930-69415949 Msa0517700:CDS 45.0%
!! GAGGAGAGAATGGATTGGTT+TGG - chr3_4:69415993-69416012 None:intergenic 45.0%
GAAGAGAGGAGAGAATGGAT+TGG - chr3_4:69415998-69416017 None:intergenic 45.0%
TGAAGAAGAGTGGAGAAGAG+AGG - chr3_4:69416012-69416031 None:intergenic 45.0%
AGAGAGGTAGAGGATGTGTT+GGG - chr3_4:69416059-69416078 None:intergenic 45.0%
! CGATGAGGTAGATTTTGTGG+TGG - chr3_4:69416192-69416211 None:intergenic 45.0%
! CACCGATGAGGTAGATTTTG+TGG - chr3_4:69416195-69416214 None:intergenic 45.0%
CACAAAATCTACCTCATCGG+TGG + chr3_4:69416193-69416212 Msa0517700:CDS 45.0%
AGGATCCATACATGACGAGA+TGG - chr3_4:69416235-69416254 None:intergenic 45.0%
GACGGGAAAATCTACGTCAT+CGG + chr3_4:69416331-69416350 Msa0517700:CDS 45.0%
!! TCACGAATTTCTGGTGGACT+AGG - chr3_4:69416439-69416458 None:intergenic 45.0%
TGTGAAGAAAGGGTTGTGGA+AGG + chr3_4:69416657-69416676 Msa0517700:CDS 45.0%
GTGGAAGGAGTTGAAAGGTT+TGG + chr3_4:69416672-69416691 Msa0517700:CDS 45.0%
CTACAATGGCTGATGTTGGT+GGG + chr3_4:69416725-69416744 Msa0517700:CDS 45.0%
! TGTTGGTGGGAAATTGGTTG+TGG + chr3_4:69416738-69416757 Msa0517700:CDS 45.0%
GTGGGAAATTGGTTGTGGTT+TGG + chr3_4:69416743-69416762 Msa0517700:CDS 45.0%
!! TTGTGGTTTGGGAGTGTCAA+AGG + chr3_4:69416755-69416774 Msa0517700:CDS 45.0%
CAGTTTATGGCTACGTCGTC+TGG - chr3_4:69415923-69415942 None:intergenic 50.0%
AGCCATAAACTGCCTTGCAC+GGG + chr3_4:69415931-69415950 Msa0517700:CDS 50.0%
! TGGTTTGGAAACGAGGGTGA+GGG - chr3_4:69415978-69415997 None:intergenic 50.0%
!! TTGGTTTGGAAACGAGGGTG+AGG - chr3_4:69415979-69415998 None:intergenic 50.0%
GTGGAGAAGAGAGGAGAGAA+TGG - chr3_4:69416003-69416022 None:intergenic 50.0%
AGAGGATGTGTTGGGTGGAA+GGG - chr3_4:69416051-69416070 None:intergenic 50.0%
TAGAGGATGTGTTGGGTGGA+AGG - chr3_4:69416052-69416071 None:intergenic 50.0%
GAGAGAGGTAGAGGATGTGT+TGG - chr3_4:69416060-69416079 None:intergenic 50.0%
GGTGAAGAATTGGAGAGAGG+TGG - chr3_4:69416095-69416114 None:intergenic 50.0%
GAGGGTGAAGAATTGGAGAG+AGG - chr3_4:69416098-69416117 None:intergenic 50.0%
GAGACGGTGGTTATTGTGGA+GGG - chr3_4:69416116-69416135 None:intergenic 50.0%
TTGTAGGGGAAGGAAGAGGA+GGG - chr3_4:69416147-69416166 None:intergenic 50.0%
ATTGTAGGGGAAGGAAGAGG+AGG - chr3_4:69416148-69416167 None:intergenic 50.0%
CCTCTTCCTTCCCCTACAAT+CGG + chr3_4:69416148-69416167 Msa0517700:CDS 50.0%
GGGAAAATCTACGTCATCGG+TGG + chr3_4:69416334-69416353 Msa0517700:CDS 50.0%
!! CTCCGACCAATTTGCTGATC+TGG - chr3_4:69416380-69416399 None:intergenic 50.0%
CAGATCAGCAAATTGGTCGG+AGG + chr3_4:69416378-69416397 Msa0517700:CDS 50.0%
GACTAGGAACACTTTCCCAG+CGG - chr3_4:69416423-69416442 None:intergenic 50.0%
AGACGGCAAAGTTTACGCGA+TGG + chr3_4:69416486-69416505 Msa0517700:CDS 50.0%
CTCTCCCACGCTCCATTATA+AGG - chr3_4:69416538-69416557 None:intergenic 50.0%
GTTGGGAGGGAGCTTGATTT+AGG + chr3_4:69416559-69416578 Msa0517700:CDS 50.0%
GGAGGGAGCTTGATTTAGGT+TGG + chr3_4:69416563-69416582 Msa0517700:CDS 50.0%
!! GAGCTTGATTTAGGTTGGCG+CGG + chr3_4:69416568-69416587 Msa0517700:CDS 50.0%
!! AGCTTGATTTAGGTTGGCGC+GGG + chr3_4:69416569-69416588 Msa0517700:CDS 50.0%
GGAGAGCAACTGTTGTGGAT+GGG + chr3_4:69416590-69416609 Msa0517700:CDS 50.0%
GGGTTGTGGAAGGAGTTGAA+AGG + chr3_4:69416667-69416686 Msa0517700:CDS 50.0%
GTTTCTGTGTGGGGCTACAA+TGG + chr3_4:69416711-69416730 Msa0517700:CDS 50.0%
GCTACAATGGCTGATGTTGG+TGG + chr3_4:69416724-69416743 Msa0517700:CDS 50.0%
! GGCTGATGTTGGTGGGAAAT+TGG + chr3_4:69416732-69416751 Msa0517700:CDS 50.0%
CAGTTTATGGCTACGTCGTC+TGG - chr3_4:69415923-69415942 None:intergenic 50.0%
AGCCATAAACTGCCTTGCAC+GGG + chr3_4:69415931-69415950 Msa0517700:CDS 50.0%
! TGGTTTGGAAACGAGGGTGA+GGG - chr3_4:69415978-69415997 None:intergenic 50.0%
!! TTGGTTTGGAAACGAGGGTG+AGG - chr3_4:69415979-69415998 None:intergenic 50.0%
GTGGAGAAGAGAGGAGAGAA+TGG - chr3_4:69416003-69416022 None:intergenic 50.0%
AGAGGATGTGTTGGGTGGAA+GGG - chr3_4:69416051-69416070 None:intergenic 50.0%
TAGAGGATGTGTTGGGTGGA+AGG - chr3_4:69416052-69416071 None:intergenic 50.0%
GAGAGAGGTAGAGGATGTGT+TGG - chr3_4:69416060-69416079 None:intergenic 50.0%
GGTGAAGAATTGGAGAGAGG+TGG - chr3_4:69416095-69416114 None:intergenic 50.0%
GAGGGTGAAGAATTGGAGAG+AGG - chr3_4:69416098-69416117 None:intergenic 50.0%
GAGACGGTGGTTATTGTGGA+GGG - chr3_4:69416116-69416135 None:intergenic 50.0%
TTGTAGGGGAAGGAAGAGGA+GGG - chr3_4:69416147-69416166 None:intergenic 50.0%
ATTGTAGGGGAAGGAAGAGG+AGG - chr3_4:69416148-69416167 None:intergenic 50.0%
CCTCTTCCTTCCCCTACAAT+CGG + chr3_4:69416148-69416167 Msa0517700:CDS 50.0%
GGGAAAATCTACGTCATCGG+TGG + chr3_4:69416334-69416353 Msa0517700:CDS 50.0%
!! CTCCGACCAATTTGCTGATC+TGG - chr3_4:69416380-69416399 None:intergenic 50.0%
CAGATCAGCAAATTGGTCGG+AGG + chr3_4:69416378-69416397 Msa0517700:CDS 50.0%
GACTAGGAACACTTTCCCAG+CGG - chr3_4:69416423-69416442 None:intergenic 50.0%
AGACGGCAAAGTTTACGCGA+TGG + chr3_4:69416486-69416505 Msa0517700:CDS 50.0%
CTCTCCCACGCTCCATTATA+AGG - chr3_4:69416538-69416557 None:intergenic 50.0%
GTTGGGAGGGAGCTTGATTT+AGG + chr3_4:69416559-69416578 Msa0517700:CDS 50.0%
GGAGGGAGCTTGATTTAGGT+TGG + chr3_4:69416563-69416582 Msa0517700:CDS 50.0%
!! GAGCTTGATTTAGGTTGGCG+CGG + chr3_4:69416568-69416587 Msa0517700:CDS 50.0%
!! AGCTTGATTTAGGTTGGCGC+GGG + chr3_4:69416569-69416588 Msa0517700:CDS 50.0%
GGAGAGCAACTGTTGTGGAT+GGG + chr3_4:69416590-69416609 Msa0517700:CDS 50.0%
GGGTTGTGGAAGGAGTTGAA+AGG + chr3_4:69416667-69416686 Msa0517700:CDS 50.0%
GTTTCTGTGTGGGGCTACAA+TGG + chr3_4:69416711-69416730 Msa0517700:CDS 50.0%
GCTACAATGGCTGATGTTGG+TGG + chr3_4:69416724-69416743 Msa0517700:CDS 50.0%
! GGCTGATGTTGGTGGGAAAT+TGG + chr3_4:69416732-69416751 Msa0517700:CDS 50.0%
TATGGCTACGTCGTCTGGGA+GGG - chr3_4:69415918-69415937 None:intergenic 55.0%
TTATGGCTACGTCGTCTGGG+AGG - chr3_4:69415919-69415938 None:intergenic 55.0%
GACCCGTGCAAGGCAGTTTA+TGG - chr3_4:69415936-69415955 None:intergenic 55.0%
AAACGAGGGTGAGGGTTGTG+TGG - chr3_4:69415970-69415989 None:intergenic 55.0%
GAGGTAGAGGATGTGTTGGG+TGG - chr3_4:69416056-69416075 None:intergenic 55.0%
GGAGACGGTGGTTATTGTGG+AGG - chr3_4:69416117-69416136 None:intergenic 55.0%
GGAGGAGACGGTGGTTATTG+TGG - chr3_4:69416120-69416139 None:intergenic 55.0%
CCGATTGTAGGGGAAGGAAG+AGG - chr3_4:69416151-69416170 None:intergenic 55.0%
GTAAGCGGAGCCGATTGTAG+GGG - chr3_4:69416161-69416180 None:intergenic 55.0%
CGTAAGCGGAGCCGATTGTA+GGG - chr3_4:69416162-69416181 None:intergenic 55.0%
!! GTGGTGGATGACAGCGTAAG+CGG - chr3_4:69416176-69416195 None:intergenic 55.0%
! CGTTGACTGAACCACCGATG+AGG - chr3_4:69416207-69416226 None:intergenic 55.0%
AGCGGTGGAATCGGCAATCA+AGG - chr3_4:69416255-69416274 None:intergenic 55.0%
!! TTGATTGCCGATTCCACCGC+TGG + chr3_4:69416254-69416273 Msa0517700:CDS 55.0%
!! GCAGCTGCTGGTGTTATCGA+CGG + chr3_4:69416313-69416332 Msa0517700:CDS 55.0%
!! CAGCTGCTGGTGTTATCGAC+GGG + chr3_4:69416314-69416333 Msa0517700:CDS 55.0%
TCGATCCTGTTACCAACCGC+TGG + chr3_4:69416404-69416423 Msa0517700:CDS 55.0%
CGATCCTGTTACCAACCGCT+GGG + chr3_4:69416405-69416424 Msa0517700:CDS 55.0%
!! CTTTCCCAGCGGTTGGTAAC+AGG - chr3_4:69416412-69416431 None:intergenic 55.0%
AGGAACACTTTCCCAGCGGT+TGG - chr3_4:69416419-69416438 None:intergenic 55.0%
GAAATGGATGCACGCTAGCG+CGG + chr3_4:69416459-69416478 Msa0517700:CDS 55.0%
CGGCAAAGTTTACGCGATGG+CGG + chr3_4:69416489-69416508 Msa0517700:CDS 55.0%
GGGAGAGCAACTGTTGTGGA+TGG + chr3_4:69416589-69416608 Msa0517700:CDS 55.0%
GTAGCCCCACACAGAAACCT+TGG - chr3_4:69416709-69416728 None:intergenic 55.0%
GGGGCTACAATGGCTGATGT+TGG + chr3_4:69416721-69416740 Msa0517700:CDS 55.0%
TATGGCTACGTCGTCTGGGA+GGG - chr3_4:69415918-69415937 None:intergenic 55.0%
TTATGGCTACGTCGTCTGGG+AGG - chr3_4:69415919-69415938 None:intergenic 55.0%
GACCCGTGCAAGGCAGTTTA+TGG - chr3_4:69415936-69415955 None:intergenic 55.0%
AAACGAGGGTGAGGGTTGTG+TGG - chr3_4:69415970-69415989 None:intergenic 55.0%
GAGGTAGAGGATGTGTTGGG+TGG - chr3_4:69416056-69416075 None:intergenic 55.0%
GGAGACGGTGGTTATTGTGG+AGG - chr3_4:69416117-69416136 None:intergenic 55.0%
GGAGGAGACGGTGGTTATTG+TGG - chr3_4:69416120-69416139 None:intergenic 55.0%
CCGATTGTAGGGGAAGGAAG+AGG - chr3_4:69416151-69416170 None:intergenic 55.0%
GTAAGCGGAGCCGATTGTAG+GGG - chr3_4:69416161-69416180 None:intergenic 55.0%
CGTAAGCGGAGCCGATTGTA+GGG - chr3_4:69416162-69416181 None:intergenic 55.0%
!! GTGGTGGATGACAGCGTAAG+CGG - chr3_4:69416176-69416195 None:intergenic 55.0%
! CGTTGACTGAACCACCGATG+AGG - chr3_4:69416207-69416226 None:intergenic 55.0%
AGCGGTGGAATCGGCAATCA+AGG - chr3_4:69416255-69416274 None:intergenic 55.0%
!! TTGATTGCCGATTCCACCGC+TGG + chr3_4:69416254-69416273 Msa0517700:CDS 55.0%
!! GCAGCTGCTGGTGTTATCGA+CGG + chr3_4:69416313-69416332 Msa0517700:CDS 55.0%
!! CAGCTGCTGGTGTTATCGAC+GGG + chr3_4:69416314-69416333 Msa0517700:CDS 55.0%
TCGATCCTGTTACCAACCGC+TGG + chr3_4:69416404-69416423 Msa0517700:CDS 55.0%
CGATCCTGTTACCAACCGCT+GGG + chr3_4:69416405-69416424 Msa0517700:CDS 55.0%
!! CTTTCCCAGCGGTTGGTAAC+AGG - chr3_4:69416412-69416431 None:intergenic 55.0%
AGGAACACTTTCCCAGCGGT+TGG - chr3_4:69416419-69416438 None:intergenic 55.0%
GAAATGGATGCACGCTAGCG+CGG + chr3_4:69416459-69416478 Msa0517700:CDS 55.0%
CGGCAAAGTTTACGCGATGG+CGG + chr3_4:69416489-69416508 Msa0517700:CDS 55.0%
GGGAGAGCAACTGTTGTGGA+TGG + chr3_4:69416589-69416608 Msa0517700:CDS 55.0%
GTAGCCCCACACAGAAACCT+TGG - chr3_4:69416709-69416728 None:intergenic 55.0%
GGGGCTACAATGGCTGATGT+TGG + chr3_4:69416721-69416740 Msa0517700:CDS 55.0%
CTACGTCGTCTGGGAGGGAT+GGG - chr3_4:69415913-69415932 None:intergenic 60.0%
! GGGTTGTGTGGTGAGAGCGT+GGG - chr3_4:69415958-69415977 None:intergenic 60.0%
AGGGTTGTGTGGTGAGAGCG+TGG - chr3_4:69415959-69415978 None:intergenic 60.0%
GAGCGAGCGGTGAAGAAGAG+TGG - chr3_4:69416022-69416041 None:intergenic 60.0%
GGAAGGGATGAGAGAGCGAG+CGG - chr3_4:69416035-69416054 None:intergenic 60.0%
GTGTGCGGAGAGAGAGGTAG+AGG - chr3_4:69416069-69416088 None:intergenic 60.0%
AAGGAAGAGGAGGGAGAGGG+AGG - chr3_4:69416138-69416157 None:intergenic 60.0%
GGGAAGGAAGAGGAGGGAGA+GGG - chr3_4:69416141-69416160 None:intergenic 60.0%
GCGTAAGCGGAGCCGATTGT+AGG - chr3_4:69416163-69416182 None:intergenic 60.0%
!! GTTTACGCGATGGCGGATCG+CGG + chr3_4:69416496-69416515 Msa0517700:CDS 60.0%
! AATGGAGCGTGGGAGAGCGT+TGG + chr3_4:69416541-69416560 Msa0517700:CDS 60.0%
!! ATGGAGCGTGGGAGAGCGTT+GGG + chr3_4:69416542-69416561 Msa0517700:CDS 60.0%
CTACGTCGTCTGGGAGGGAT+GGG - chr3_4:69415913-69415932 None:intergenic 60.0%
! GGGTTGTGTGGTGAGAGCGT+GGG - chr3_4:69415958-69415977 None:intergenic 60.0%
AGGGTTGTGTGGTGAGAGCG+TGG - chr3_4:69415959-69415978 None:intergenic 60.0%
GAGCGAGCGGTGAAGAAGAG+TGG - chr3_4:69416022-69416041 None:intergenic 60.0%
GGAAGGGATGAGAGAGCGAG+CGG - chr3_4:69416035-69416054 None:intergenic 60.0%
GTGTGCGGAGAGAGAGGTAG+AGG - chr3_4:69416069-69416088 None:intergenic 60.0%
AAGGAAGAGGAGGGAGAGGG+AGG - chr3_4:69416138-69416157 None:intergenic 60.0%
GGGAAGGAAGAGGAGGGAGA+GGG - chr3_4:69416141-69416160 None:intergenic 60.0%
GCGTAAGCGGAGCCGATTGT+AGG - chr3_4:69416163-69416182 None:intergenic 60.0%
!! GTTTACGCGATGGCGGATCG+CGG + chr3_4:69416496-69416515 Msa0517700:CDS 60.0%
! AATGGAGCGTGGGAGAGCGT+TGG + chr3_4:69416541-69416560 Msa0517700:CDS 60.0%
!! ATGGAGCGTGGGAGAGCGTT+GGG + chr3_4:69416542-69416561 Msa0517700:CDS 60.0%
CGTCTGGGAGGGATGGGATC+AGG - chr3_4:69415907-69415926 None:intergenic 65.0%
GCTACGTCGTCTGGGAGGGA+TGG - chr3_4:69415914-69415933 None:intergenic 65.0%
TGGAGCGTGTGCGGAGAGAG+AGG - chr3_4:69416075-69416094 None:intergenic 65.0%
GGAGAGAGGTGGAGCGTGTG+CGG - chr3_4:69416084-69416103 None:intergenic 65.0%
GAGGAGGGAGAGGGAGGAGA+CGG - chr3_4:69416132-69416151 None:intergenic 65.0%
GGGGAAGGAAGAGGAGGGAG+AGG - chr3_4:69416142-69416161 None:intergenic 65.0%
GCGGAGCCGATTGTAGGGGA+AGG - chr3_4:69416157-69416176 None:intergenic 65.0%
GAATTCACGCGCCACCCGCA+TGG - chr3_4:69416293-69416312 None:intergenic 65.0%
GCGCGTGAATTCGCAGCTGC+TGG + chr3_4:69416301-69416320 Msa0517700:CDS 65.0%
CGTCATCGGTGGTTGCGTGC+CGG + chr3_4:69416345-69416364 Msa0517700:CDS 65.0%
CACGCTAGCGCGGTCGTAGA+CGG + chr3_4:69416469-69416488 Msa0517700:CDS 65.0%
!! AGCGTGGGAGAGCGTTGGGA+GGG + chr3_4:69416546-69416565 Msa0517700:CDS 65.0%
GCGCGGGAGAGCAACTGTTG+TGG + chr3_4:69416585-69416604 Msa0517700:CDS 65.0%
CGTCTGGGAGGGATGGGATC+AGG - chr3_4:69415907-69415926 None:intergenic 65.0%
GCTACGTCGTCTGGGAGGGA+TGG - chr3_4:69415914-69415933 None:intergenic 65.0%
TGGAGCGTGTGCGGAGAGAG+AGG - chr3_4:69416075-69416094 None:intergenic 65.0%
GGAGAGAGGTGGAGCGTGTG+CGG - chr3_4:69416084-69416103 None:intergenic 65.0%
GAGGAGGGAGAGGGAGGAGA+CGG - chr3_4:69416132-69416151 None:intergenic 65.0%
GGGGAAGGAAGAGGAGGGAG+AGG - chr3_4:69416142-69416161 None:intergenic 65.0%
GCGGAGCCGATTGTAGGGGA+AGG - chr3_4:69416157-69416176 None:intergenic 65.0%
GAATTCACGCGCCACCCGCA+TGG - chr3_4:69416293-69416312 None:intergenic 65.0%
GCGCGTGAATTCGCAGCTGC+TGG + chr3_4:69416301-69416320 Msa0517700:CDS 65.0%
CGTCATCGGTGGTTGCGTGC+CGG + chr3_4:69416345-69416364 Msa0517700:CDS 65.0%
CACGCTAGCGCGGTCGTAGA+CGG + chr3_4:69416469-69416488 Msa0517700:CDS 65.0%
!! AGCGTGGGAGAGCGTTGGGA+GGG + chr3_4:69416546-69416565 Msa0517700:CDS 65.0%
GCGCGGGAGAGCAACTGTTG+TGG + chr3_4:69416585-69416604 Msa0517700:CDS 65.0%
! GAGAGCGTGGGACCCGTGCA+AGG - chr3_4:69415946-69415965 None:intergenic 70.0%
GAGGGAGAGGGAGGAGACGG+TGG - chr3_4:69416129-69416148 None:intergenic 70.0%
CGGGGAGCCAGCGGTGGAAT+CGG - chr3_4:69416264-69416283 None:intergenic 70.0%
!! CGATTCCACCGCTGGCTCCC+CGG + chr3_4:69416262-69416281 Msa0517700:CDS 70.0%
TCACGCGCCACCCGCATGGA+GGG - chr3_4:69416289-69416308 None:intergenic 70.0%
TTCACGCGCCACCCGCATGG+AGG - chr3_4:69416290-69416309 None:intergenic 70.0%
!! TACGCGATGGCGGATCGCGG+TGG + chr3_4:69416499-69416518 Msa0517700:CDS 70.0%
!! GAGCGTGGGAGAGCGTTGGG+AGG + chr3_4:69416545-69416564 Msa0517700:CDS 70.0%
! GAGAGCGTGGGACCCGTGCA+AGG - chr3_4:69415946-69415965 None:intergenic 70.0%
GAGGGAGAGGGAGGAGACGG+TGG - chr3_4:69416129-69416148 None:intergenic 70.0%
CGGGGAGCCAGCGGTGGAAT+CGG - chr3_4:69416264-69416283 None:intergenic 70.0%
!! CGATTCCACCGCTGGCTCCC+CGG + chr3_4:69416262-69416281 Msa0517700:CDS 70.0%
TCACGCGCCACCCGCATGGA+GGG - chr3_4:69416289-69416308 None:intergenic 70.0%
TTCACGCGCCACCCGCATGG+AGG - chr3_4:69416290-69416309 None:intergenic 70.0%
!! TACGCGATGGCGGATCGCGG+TGG + chr3_4:69416499-69416518 Msa0517700:CDS 70.0%
!! GAGCGTGGGAGAGCGTTGGG+AGG + chr3_4:69416545-69416564 Msa0517700:CDS 70.0%
TGGAGGGACCGGGGAGCCAG+CGG - chr3_4:69416273-69416292 None:intergenic 75.0%
GGCTCCCCGGTCCCTCCATG+CGG + chr3_4:69416275-69416294 Msa0517700:CDS 75.0%
GCTCCCCGGTCCCTCCATGC+GGG + chr3_4:69416276-69416295 Msa0517700:CDS 75.0%
CCACCCGCATGGAGGGACCG+GGG - chr3_4:69416282-69416301 None:intergenic 75.0%
GCCACCCGCATGGAGGGACC+GGG - chr3_4:69416283-69416302 None:intergenic 75.0%
CGCCACCCGCATGGAGGGAC+CGG - chr3_4:69416284-69416303 None:intergenic 75.0%
TGGAGGGACCGGGGAGCCAG+CGG - chr3_4:69416273-69416292 None:intergenic 75.0%
GGCTCCCCGGTCCCTCCATG+CGG + chr3_4:69416275-69416294 Msa0517700:CDS 75.0%
GCTCCCCGGTCCCTCCATGC+GGG + chr3_4:69416276-69416295 Msa0517700:CDS 75.0%
CCACCCGCATGGAGGGACCG+GGG - chr3_4:69416282-69416301 None:intergenic 75.0%
GCCACCCGCATGGAGGGACC+GGG - chr3_4:69416283-69416302 None:intergenic 75.0%
CGCCACCCGCATGGAGGGAC+CGG - chr3_4:69416284-69416303 None:intergenic 75.0%
! AGGGACCGGGGAGCCAGCGG+TGG - chr3_4:69416270-69416289 None:intergenic 80.0%
! CCCCGGTCCCTCCATGCGGG+TGG + chr3_4:69416279-69416298 Msa0517700:CDS 80.0%
! AGGGACCGGGGAGCCAGCGG+TGG - chr3_4:69416270-69416289 None:intergenic 80.0%
! CCCCGGTCCCTCCATGCGGG+TGG + chr3_4:69416279-69416298 Msa0517700:CDS 80.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 69415881 69416951 69415881 ID=Msa0517700;Name=Msa0517700
chr3_4 mRNA 69415881 69416951 69415881 ID=Msa0517700-mRNA-1;Parent=Msa0517700;Name=Msa0517700-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|-1|0|1|-1|1|1|0|356
chr3_4 exon 69415881 69416951 69415881 ID=Msa0517700-mRNA-1:exon:18500;Parent=Msa0517700-mRNA-1
chr3_4 CDS 69415881 69416951 69415881 ID=Msa0517700-mRNA-1:cds;Parent=Msa0517700-mRNA-1
Gene Sequence

>Msa0517700

ATGAAGAAGAACCAAAACTCAAACCTGATCCCATCCCTCCCAGACGACGTAGCCATAAACTGCCTTGCACGGGTCCCACGCTCTCACCACACAACCCTCACCCTCGTTTCCAAACCAATCCATTCTCTCCTCTCTTCTCCACTCTTCTTCACCGCTCGCTCTCTCATCCCTTCCACCCAACACATCCTCTACCTCTCTCTCCGCACACGCTCCACCTCTCTCCAATTCTTCACCCTCCACAATAACCACCGTCTCCTCCCTCTCCCTCCTCTTCCTTCCCCTACAATCGGCTCCGCTTACGCTGTCATCCACCACAAAATCTACCTCATCGGTGGTTCAGTCAACGATGTACCATCTCGTCATGTATGGATCCTTGATTGCCGATTCCACCGCTGGCTCCCCGGTCCCTCCATGCGGGTGGCGCGTGAATTCGCAGCTGCTGGTGTTATCGACGGGAAAATCTACGTCATCGGTGGTTGCGTGCCGGATAATTTTTCCAGATCAGCAAATTGGTCGGAGGTTTTCGATCCTGTTACCAACCGCTGGGAAAGTGTTCCTAGTCCACCAGAAATTCGTGAGAAATGGATGCACGCTAGCGCGGTCGTAGACGGCAAAGTTTACGCGATGGCGGATCGCGGTGGTGTTTTGTTTGATCCTTATAATGGAGCGTGGGAGAGCGTTGGGAGGGAGCTTGATTTAGGTTGGCGCGGGAGAGCAACTGTTGTGGATGGGATTTTGTATTGCTATGATTATTTAGGGAAAATTAAGGGTTTTGATGTGAAGAAAGGGTTGTGGAAGGAGTTGAAAGGTTTGGATAAGAGTTTACCAAGGTTTCTGTGTGGGGCTACAATGGCTGATGTTGGTGGGAAATTGGTTGTGGTTTGGGAGTGTCAAAGGAATTTGAATGCAAATAAGAAGGGGAAAGAAATGGAGATTTGGTGTGCTGAGATTGATGTGAAGAAGAAGAAGAATAAGGATGGGGAATTGTGGGGTGAAGTTTGTTGGTTGAATAATGTTCTTTCTGTTCCTAAAGGATCTTCCATTGTTCATTGTTCTTCTGTTGCATTATGA
Protein sequence

>Msa0517700

MKKNQNSNLIPSLPDDVAINCLARVPRSHHTTLTLVSKPIHSLLSSPLFFTARSLIPSTQHILYLSLRTRSTSLQFFTLHNNHRLLPLPPLPSPTIGSAYAVIHHKIYLIGGSVNDVPSRHVWILDCRFHRWLPGPSMRVAREFAAAGVIDGKIYVIGGCVPDNFSRSANWSEVFDPVTNRWESVPSPPEIREKWMHASAVVDGKVYAMADRGGVLFDPYNGAWESVGRELDLGWRGRATVVDGILYCYDYLGKIKGFDVKKGLWKELKGLDKSLPRFLCGATMADVGGKLVVVWECQRNLNANKKGKEMEIWCAEIDVKKKKNKDGELWGEVCWLNNVLSVPKGSSIVHCSSVAL*