AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0531890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0531890 MtrunA17_Chr2g0281621 94.845 97 5 0 95 191 122 218 8.71e-58 194
Msa0531890 MtrunA17_Chr3g0100041 86.087 115 11 1 239 348 306 420 1.02e-56 190
Msa0531890 MtrunA17_Chr3g0099991 81.739 115 16 1 239 348 339 453 4.91e-54 184
Msa0531890 MtrunA17_Chr4g0047861 83.168 101 17 0 95 195 142 242 2.31e-51 177
Msa0531890 MtrunA17_Chr5g0444771 77.391 115 21 1 239 348 410 524 8.85e-51 177
Msa0531890 MtrunA17_Chr2g0281641 80.412 97 19 0 95 191 42 138 5.95e-50 171
Msa0531890 MtrunA17_Chr4g0075521 73.043 115 26 1 239 348 409 523 1.49e-48 171
Msa0531890 MtrunA17_Chr2g0289411 71.681 113 27 1 241 348 409 521 2.90e-46 165
Msa0531890 MtrunA17_Chr4g0068861 52.336 107 49 1 87 191 163 269 1.06e-31 124
Msa0531890 MtrunA17_Chr4g0068841 55.556 108 45 2 87 191 117 224 1.19e-31 124
Msa0531890 MtrunA17_Chr4g0038311 48.837 129 60 3 64 189 93 218 3.08e-31 122
Msa0531890 MtrunA17_Chr7g0221551 52.586 116 50 2 79 190 95 209 2.52e-29 117
Msa0531890 MtrunA17_Chr3g0118841 57.009 107 40 3 87 189 157 261 2.64e-28 115
Msa0531890 MtrunA17_Chr8g0334871 52.991 117 36 3 2 114 122 223 8.60e-28 109
Msa0531890 MtrunA17_Chr3g0118891 54.286 105 46 2 87 189 156 260 7.48e-27 110
Msa0531890 MtrunA17_Chr3g0118831 54.206 107 43 3 87 189 156 260 1.60e-26 109
Msa0531890 MtrunA17_Chr1g0199991 56.250 96 37 2 97 189 124 217 1.96e-26 109
Msa0531890 MtrunA17_Chr1g0165811 39.355 155 82 4 82 228 101 251 2.11e-26 105
Msa0531890 MtrunA17_Chr4g0020241 56.989 93 40 0 97 189 162 254 2.96e-26 108
Msa0531890 MtrunA17_Chr1g0153931 56.044 91 38 1 97 185 165 255 2.99e-26 105
Msa0531890 MtrunA17_Chr3g0118881 58.947 95 36 2 97 189 167 260 3.09e-26 108
Msa0531890 MtrunA17_Chr3g0118851 55.140 107 42 3 87 189 156 260 3.21e-26 108
Msa0531890 MtrunA17_Chr3g0118861 61.957 92 30 2 97 185 165 254 5.25e-26 108
Msa0531890 MtrunA17_Chr1g0199971 54.167 96 39 2 97 189 124 217 3.40e-25 105
Msa0531890 MtrunA17_Chr5g0405131 85.714 56 8 0 2 57 8 63 4.32e-25 99.8
Msa0531890 MtrunA17_Chr5g0405131 91.176 34 3 0 161 194 134 167 3.19e-13 67.0
Msa0531890 MtrunA17_Chr1g0153881 52.632 95 43 1 97 189 179 273 6.02e-25 105
Msa0531890 MtrunA17_Chr3g0090331 56.383 94 40 1 97 189 163 256 1.10e-24 104
Msa0531890 MtrunA17_Chr3g0126051 29.365 252 154 7 95 330 120 363 1.45e-24 103
Msa0531890 MtrunA17_Chr5g0431301 49.485 97 47 2 95 190 116 211 4.34e-24 102
Msa0531890 MtrunA17_Chr2g0304931 52.128 94 41 1 97 190 127 216 1.12e-23 101
Msa0531890 MtrunA17_Chr3g0131731 52.577 97 44 2 95 190 116 211 1.30e-23 101
Msa0531890 MtrunA17_Chr3g0126031 49.474 95 46 2 97 190 117 210 2.30e-23 100
Msa0531890 MtrunA17_Chr3g0094811 51.042 96 43 1 95 190 115 206 3.92e-23 99.8
Msa0531890 MtrunA17_Chr1g0153941 52.632 95 43 1 97 189 167 261 1.28e-22 98.6
Msa0531890 MtrunA17_Chr8g0385871 44.828 116 59 2 79 190 98 212 2.07e-22 97.8
Msa0531890 MtrunA17_Chr7g0219051 49.462 93 47 0 97 189 124 216 2.59e-21 94.7
Msa0531890 MtrunA17_Chr7g0232961 57.500 80 30 1 1 76 141 220 5.03e-20 89.0
Msa0531890 MtrunA17_Chr2g0318911 45.833 96 51 1 95 189 129 224 1.03e-19 90.1
Msa0531890 MtrunA17_Chr2g0309521 45.833 96 51 1 95 189 128 223 6.59e-19 87.4
Msa0531890 MtrunA17_Chr2g0309511 40.336 119 68 2 95 210 128 246 7.37e-19 87.4
Msa0531890 MtrunA17_Chr7g0232491 62.121 66 25 0 2 67 114 179 1.77e-18 84.3
Msa0531890 MtrunA17_Chr2g0309541 44.444 99 50 3 95 189 127 224 8.73e-18 84.3
Msa0531890 MtrunA17_Chr7g0249431 41.026 117 59 3 74 189 108 215 9.58e-17 81.3
Msa0531890 MtrunA17_Chr7g0234051 46.392 97 46 3 97 190 123 216 5.02e-16 79.0
Msa0531890 MtrunA17_Chr4g0028241 40.625 96 55 2 95 189 160 254 1.03e-15 78.2
Msa0531890 MtrunA17_Chr7g0234041 43.119 109 49 5 86 190 108 207 7.49e-15 75.5
Msa0531890 MtrunA17_Chr2g0327381 37.500 96 59 1 95 189 166 261 8.47e-15 75.5
Msa0531890 MtrunA17_Chr3g0093971 34.400 125 66 5 79 190 91 212 2.71e-14 73.6
Msa0531890 MtrunA17_Chr1g0171411 40.426 94 54 2 97 189 156 248 4.77e-14 73.2
Msa0531890 MtrunA17_Chr1g0199171 37.398 123 60 5 71 189 145 254 5.18e-14 72.8
Msa0531890 MtrunA17_Chr8g0338461 44.792 96 47 4 97 189 124 216 5.35e-14 72.8
Msa0531890 MtrunA17_Chr2g0327401 38.298 94 56 2 97 189 174 266 1.04e-13 72.0
Msa0531890 MtrunA17_Chr4g0028261 38.542 96 57 2 95 189 189 283 5.04e-13 70.1
Msa0531890 MtrunA17_Chr4g0028271 36.364 121 64 4 71 189 153 262 8.75e-13 69.3
Msa0531890 MtrunA17_Chr4g0028281 37.398 123 60 5 71 189 151 260 8.84e-13 69.3
Msa0531890 MtrunA17_Chr2g0322021 49.333 75 26 2 153 226 312 375 8.03e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0531890 AT3G20290.3 77.391 115 21 1 239 348 412 526 3.48e-50 176
Msa0531890 AT3G20290.1 77.391 115 21 1 239 348 412 526 3.48e-50 176
Msa0531890 AT3G20290.2 77.391 115 21 1 239 348 412 526 3.48e-50 176
Msa0531890 AT4G05520.1 70.435 115 29 1 239 348 412 526 1.32e-45 163
Msa0531890 AT4G05520.2 70.435 115 29 1 239 348 411 525 1.37e-45 163
Msa0531890 AT1G61130.1 65.741 108 33 2 87 190 109 216 1.84e-39 145
Msa0531890 AT1G11080.2 67.925 106 29 1 95 195 137 242 6.78e-38 142
Msa0531890 AT1G11080.1 67.925 106 29 1 95 195 137 242 6.79e-38 142
Msa0531890 AT4G15100.1 54.135 133 46 3 95 213 131 262 1.10e-35 135
Msa0531890 AT3G17180.2 60.606 99 37 1 94 190 126 224 1.39e-31 124
Msa0531890 AT3G17180.1 60.606 99 37 1 94 190 126 224 2.41e-31 123
Msa0531890 AT2G35770.1 51.261 119 51 3 97 210 127 243 4.00e-29 117
Msa0531890 AT5G23210.4 53.271 107 47 2 86 189 33 139 1.69e-28 114
Msa0531890 AT5G08260.1 55.670 97 41 1 95 189 127 223 2.29e-28 115
Msa0531890 AT2G33530.1 50.862 116 52 2 79 190 98 212 2.50e-28 115
Msa0531890 AT5G23210.2 53.271 107 47 2 86 189 33 139 4.10e-28 113
Msa0531890 AT5G23210.5 53.271 107 47 2 86 189 34 140 4.19e-28 113
Msa0531890 AT5G23210.3 53.271 107 47 2 86 189 129 235 7.56e-28 113
Msa0531890 AT1G28110.1 50.862 116 52 2 79 190 96 210 9.10e-28 113
Msa0531890 AT1G28110.2 50.862 116 52 2 79 190 96 210 9.10e-28 113
Msa0531890 AT5G23210.1 53.271 107 47 2 86 189 129 235 1.17e-27 113
Msa0531890 AT3G07990.1 56.250 96 37 2 97 189 125 218 6.76e-26 108
Msa0531890 AT2G24000.1 55.556 99 39 2 96 191 119 215 1.13e-25 107
Msa0531890 AT2G35780.1 55.914 93 38 2 97 189 121 210 3.21e-25 106
Msa0531890 AT3G52000.1 55.208 96 40 2 96 191 151 243 1.77e-24 104
Msa0531890 AT3G52020.1 55.319 94 41 1 97 190 167 259 2.09e-24 104
Msa0531890 AT2G05850.1 48.980 98 45 2 96 190 152 247 7.02e-23 99.8
Msa0531890 AT3G63470.1 54.839 93 39 1 97 189 170 259 1.06e-22 99.4
Msa0531890 AT3G52010.1 52.041 98 42 2 96 190 152 247 1.23e-22 99.0
Msa0531890 AT4G30610.1 46.552 116 58 2 95 206 120 235 3.70e-22 97.4
Msa0531890 AT1G43780.1 47.959 98 48 2 95 190 124 220 6.66e-22 96.7
Msa0531890 AT4G30810.1 48.958 96 48 1 95 189 125 220 7.90e-22 96.7
Msa0531890 AT2G24010.4 50.000 96 46 1 96 189 104 199 1.80e-20 91.7
Msa0531890 AT2G24010.2 50.000 96 46 1 96 189 133 228 2.98e-20 92.0
Msa0531890 AT2G24010.1 50.000 96 46 1 96 189 118 213 3.35e-20 91.7
Msa0531890 AT2G12480.4 45.361 97 51 2 95 190 118 213 4.43e-20 91.3
Msa0531890 AT2G12480.1 45.361 97 51 2 95 190 118 213 4.51e-20 91.3
Msa0531890 AT2G12480.2 45.361 97 51 2 95 190 118 213 4.54e-20 91.3
Msa0531890 AT3G02110.1 47.312 93 49 0 97 189 126 218 4.63e-20 91.3
Msa0531890 AT2G12480.3 45.361 97 51 2 95 190 118 213 4.75e-20 91.3
Msa0531890 AT5G42240.1 44.330 97 52 2 95 190 119 214 1.06e-19 90.5
Msa0531890 AT2G24010.3 48.936 94 46 1 96 187 104 197 1.48e-18 87.0
Msa0531890 AT3G56540.1 60.606 66 26 0 97 162 167 232 2.53e-18 84.0
Msa0531890 AT4G12910.2 46.809 94 48 2 97 189 128 220 4.83e-18 85.5
Msa0531890 AT4G12910.1 46.809 94 48 2 97 189 128 220 6.16e-18 85.1
Msa0531890 AT3G25420.1 46.809 94 48 2 97 189 121 213 8.77e-18 84.7
Msa0531890 AT5G42230.1 42.268 97 54 2 95 190 115 210 9.11e-18 84.7
Msa0531890 AT5G42230.2 42.268 97 54 2 95 190 115 210 9.11e-18 84.7
Msa0531890 AT3G45010.1 39.496 119 61 5 79 189 150 265 1.94e-15 77.8
Msa0531890 AT3G10410.1 41.837 98 51 3 95 189 169 263 2.18e-14 74.7
Msa0531890 AT3G60450.1 37.815 119 51 2 2 114 110 211 1.20e-13 70.9
Msa0531890 AT3G60450.2 37.815 119 51 2 2 114 142 243 1.31e-13 70.9
Msa0531890 AT3G60420.1 38.583 127 66 3 2 128 124 238 1.84e-13 70.1
Msa0531890 AT2G23010.1 39.000 100 54 3 94 190 115 210 1.29e-12 68.9
Msa0531890 AT2G23010.2 39.000 100 54 3 94 190 115 210 1.36e-12 68.9
Msa0531890 AT1G73290.2 37.755 98 59 2 94 190 122 218 2.11e-12 67.4
Msa0531890 AT5G22980.1 34.959 123 63 5 97 205 170 289 3.48e-12 67.8
Msa0531890 AT3G60440.2 54.386 57 26 0 2 58 133 189 4.66e-12 65.9
Msa0531890 AT1G33540.2 37.895 95 57 2 97 190 79 172 4.84e-12 67.0
Msa0531890 AT1G33540.1 37.895 95 57 2 97 190 122 215 5.53e-12 67.0
Msa0531890 AT2G23000.1 36.893 103 62 2 97 198 118 218 8.83e-12 66.2
Msa0531890 AT2G23000.2 36.893 103 62 2 97 198 118 218 8.83e-12 66.2
Msa0531890 AT1G73290.3 37.755 98 59 2 94 190 122 218 9.74e-12 66.2
Msa0531890 AT3G60440.1 54.386 57 26 0 2 58 133 189 1.19e-11 65.1
Msa0531890 AT1G73290.1 37.755 98 59 2 94 190 122 218 1.23e-11 65.9
Msa0531890 AT2G22980.3 38.144 97 55 2 94 190 118 209 1.37e-11 65.5
Msa0531890 AT3G60415.1 56.140 57 25 0 2 58 109 165 1.38e-11 64.7
Msa0531890 AT1G73300.1 37.000 100 57 3 94 190 123 219 3.79e-11 64.3
Msa0531890 AT1G73300.2 37.000 100 57 3 94 190 123 219 3.79e-11 64.3
Msa0531890 AT2G22980.5 38.298 94 53 2 97 190 2 90 4.14e-11 63.5
Msa0531890 AT2G22980.4 38.298 94 53 2 97 190 119 207 4.71e-11 64.3
Msa0531890 AT2G22980.1 38.298 94 53 2 97 190 119 207 6.30e-11 63.5
Msa0531890 AT2G22980.2 38.298 94 53 2 97 190 119 207 7.21e-11 63.5

Find 97 sgRNAs with CRISPR-Local

Find 323 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAATGAGAGGATCTATTT+TGG 0.177743 4_1:+5992352 Msa0531890:three_prime_UTR
GATAATATATGCATGTATTT+TGG 0.206206 4_1:-5991956 None:intergenic
TATTGATCTCAAGGGCATTT+TGG 0.225051 4_1:+5990321 Msa0531890:CDS
CTGCAGGTCTCTGTAGAATA+TGG 0.240516 4_1:+5987713 Msa0531890:intron
GCCAATCCTGCTCACTCTTC+AGG 0.248426 4_1:-5990471 None:intergenic
TGGCTCCTAAAGATGAAAAT+TGG 0.260885 4_1:+5987777 Msa0531890:CDS
TTATGATCACAAACCTTCTT+TGG 0.264058 4_1:-5991255 None:intergenic
CTCCATATGAGTGGCAAATA+TGG 0.266024 4_1:-5988787 None:intergenic
GATTAGGAATGTCCTTAAAA+TGG 0.291784 4_1:-5992327 None:intergenic
GCCACGTACCTGCAAATAAA+AGG 0.291918 4_1:-5992497 None:intergenic
AGAAGCAAACATGTTATTCT+TGG 0.302955 4_1:+5989860 Msa0531890:intron
TCAAATTGCATTCTTCTGTT+TGG 0.342684 4_1:+5990685 Msa0531890:CDS
TCGACAACTTGTATCAATTT+CGG 0.347320 4_1:-5992254 None:intergenic
AAGCAAATGCCTATCGTGAT+AGG 0.362210 4_1:+5991991 Msa0531890:CDS
GGCTCCTAAAGATGAAAATT+GGG 0.366205 4_1:+5987778 Msa0531890:CDS
CCGCTGTTTCTATGCATTGA+AGG 0.376433 4_1:+5992623 Msa0531890:three_prime_UTR
AAATATGATTGTTAGCAAAC+GGG 0.407617 4_1:+5992535 Msa0531890:three_prime_UTR
TTGGAATCTCCTGTTGGAGT+TGG 0.412688 4_1:+5989879 Msa0531890:CDS
TGATGAGCCGGAGGGCAATT+AGG 0.415742 4_1:+5987747 Msa0531890:CDS
AATGGACAAGGACAACAATA+AGG 0.416606 4_1:+5992296 Msa0531890:three_prime_UTR
TACCATATTTGCCACTCATA+TGG 0.416671 4_1:+5988785 Msa0531890:CDS
AAACAACCATTTGGAGTTCA+AGG 0.419670 4_1:+5988875 Msa0531890:CDS
CACTCAACACCTCTTTGAAA+TGG 0.422954 4_1:-5992199 None:intergenic
CAAATATGATTGTTAGCAAA+CGG 0.429314 4_1:+5992534 Msa0531890:three_prime_UTR
AGCAGTTTCAAAGTTGTGTT+TGG 0.435209 4_1:+5990613 Msa0531890:CDS
AATGCTCTTGTTCTATATGA+TGG 0.436461 4_1:-5990107 None:intergenic
CCAATCCTGCTCACTCTTCA+GGG 0.438226 4_1:-5990470 None:intergenic
TAAGAAAATCCAACTCCAAC+AGG 0.446307 4_1:-5989888 None:intergenic
TCTTAGTTGTGCATATTCAC+TGG 0.451439 4_1:-5989922 None:intergenic
CGTGAGGCCATGCTTCCTGC+AGG 0.455082 4_1:+5987818 Msa0531890:CDS
TGAACTCTTTAAAAGAGAAC+AGG 0.466788 4_1:+5991203 Msa0531890:intron
TTATTCTTGGAATCTCCTGT+TGG 0.472255 4_1:+5989873 Msa0531890:CDS
TGCCCTTGAGATCAATATGA+AGG 0.472647 4_1:-5990315 None:intergenic
GAGGAACGCTGGAGCAGAAA+AGG 0.473807 4_1:+5986946 Msa0531890:five_prime_UTR
ATAGGAATACTATAAATCAT+AGG 0.479179 4_1:-5992710 None:intergenic
TTCCCTTCATATTGATCTCA+AGG 0.480182 4_1:+5990312 Msa0531890:CDS
TCTAAAAGATATACCAAAGA+AGG 0.481427 4_1:+5991242 Msa0531890:CDS
ATACTACCTTGAACTCCAAA+TGG 0.498641 4_1:-5988881 None:intergenic
TAAAGTCTCCGGCAGGGAGA+TGG 0.499615 4_1:-5992166 None:intergenic
AAGAGAGACGGCGAGAGGAG+AGG 0.501706 4_1:+5987015 Msa0531890:five_prime_UTR
ATAGCAGGAGAGAGCTATGC+AGG 0.504990 4_1:+5990134 Msa0531890:CDS
GTCAACATTTGTAAAGTCTC+CGG 0.507418 4_1:-5992177 None:intergenic
TGCAGGTCTCTGTAGAATAT+GGG 0.508561 4_1:+5987714 Msa0531890:intron
CACTGTCCCTGCAGGAAGCA+TGG 0.520311 4_1:-5987825 None:intergenic
ACATTTGTAAAGTCTCCGGC+AGG 0.522144 4_1:-5992173 None:intergenic
ATCCTCTCATTCAAGAGATT+AGG 0.524602 4_1:-5992343 None:intergenic
CAGGTACGTGGCAAGCGAGT+TGG 0.525525 4_1:+5992508 Msa0531890:three_prime_UTR
ATGCAATTTGAGTATCTCCT+TGG 0.525810 4_1:-5990673 None:intergenic
TTGTTATCCACTGTCCCTGC+AGG 0.531343 4_1:-5987833 None:intergenic
AGTGGATAACAATGTAGAAA+GGG 0.541133 4_1:+5987844 Msa0531890:CDS
ACACGTGTAACAGGTACAAA+GGG 0.542235 4_1:+5992139 Msa0531890:intron
TTAGCTTTCCCTATCACGAT+AGG 0.543279 4_1:-5992000 None:intergenic
CAGTGGATAACAATGTAGAA+AGG 0.543991 4_1:+5987843 Msa0531890:CDS
AGCAAATGCCTATCGTGATA+GGG 0.544684 4_1:+5991992 Msa0531890:CDS
AGGGAGTTCCATCTCCCTGC+CGG 0.550817 4_1:+5992158 Msa0531890:CDS
AGGAGAGGAAAGTGTTGTGT+TGG 0.553945 4_1:+5987030 Msa0531890:five_prime_UTR
TGTAGAAAGGGTCTATAAAG+AGG 0.560431 4_1:+5987856 Msa0531890:CDS
TTCCTAATCTCTTGAATGAG+AGG 0.563360 4_1:+5992341 Msa0531890:three_prime_UTR
AAAGAGGAAAGGAGGAACGC+TGG 0.572269 4_1:+5986935 Msa0531890:five_prime_UTR
AAATGTTGACCATTTCAAAG+AGG 0.573061 4_1:+5992190 Msa0531890:CDS
CCTCAAGCCTAATTGCCCTC+CGG 0.574132 4_1:-5987754 None:intergenic
AGTAATCGCAAGACAAGAGA+TGG 0.579715 4_1:+5990650 Msa0531890:CDS
GCCCTTGAGATCAATATGAA+GGG 0.580105 4_1:-5990314 None:intergenic
TTGAGATCAATATGAAGGGA+AGG 0.580444 4_1:-5990310 None:intergenic
TTACATGAAATGTTGCATGT+TGG 0.580927 4_1:+5990590 Msa0531890:CDS
AACTCATTGATAACCTGGAG+GGG 0.583061 4_1:+5992034 Msa0531890:CDS
CAATGAGTTTGCAAGAGCAA+TGG 0.586120 4_1:+5992278 Msa0531890:exon
CGCTATATTCTCCATATGAG+TGG 0.586227 4_1:-5988796 None:intergenic
CAACTAAGAGATGACTTCAC+AGG 0.589863 4_1:+5989936 Msa0531890:CDS
AACGAAAGAGAGACGGCGAG+AGG 0.594423 4_1:+5987010 Msa0531890:five_prime_UTR
AGAGACGGGAACGAGAGAGA+AGG 0.594857 4_1:+5986898 None:intergenic
TCCCTTCATATTGATCTCAA+GGG 0.595442 4_1:+5990313 Msa0531890:CDS
GCTGGAGCAGAAAAGGAAAA+CGG 0.595838 4_1:+5986953 Msa0531890:five_prime_UTR
ACTCATTGATAACCTGGAGG+GGG 0.601551 4_1:+5992035 Msa0531890:CDS
AAACTCATTGATAACCTGGA+GGG 0.604910 4_1:+5992033 Msa0531890:CDS
AACACGTGTAACAGGTACAA+AGG 0.609571 4_1:+5992138 Msa0531890:intron
CCGGAGGGCAATTAGGCTTG+AGG 0.610283 4_1:+5987754 Msa0531890:CDS
AATATGATTGTTAGCAAACG+GGG 0.610424 4_1:+5992536 Msa0531890:three_prime_UTR
GAGGGCAATTAGGCTTGAGG+TGG 0.611737 4_1:+5987757 Msa0531890:CDS
CAAGGACAACAATAAGGACA+TGG 0.614893 4_1:+5992302 Msa0531890:three_prime_UTR
CATGCTTCCTGCAGGGACAG+TGG 0.620249 4_1:+5987826 Msa0531890:CDS
TGAGATCAATATGAAGGGAA+GGG 0.626331 4_1:-5990309 None:intergenic
GTTTGCAAGAGCAATGGACA+AGG 0.626826 4_1:+5992284 Msa0531890:three_prime_UTR
GCTCCTAAAGATGAAAATTG+GGG 0.633816 4_1:+5987779 Msa0531890:CDS
CGCAAGACAAGAGATGGCCA+AGG 0.635388 4_1:+5990656 Msa0531890:CDS
GGTTATGTGAAATGATGAGC+CGG 0.636708 4_1:+5987735 Msa0531890:CDS
GTGAGGCCATGCTTCCTGCA+GGG 0.637114 4_1:+5987819 Msa0531890:CDS
TTTCAACATATCAGAGCGTG+AGG 0.644870 4_1:+5987802 Msa0531890:CDS
AGGTGAAAACGAAAGAGAGA+CGG 0.651922 4_1:+5987003 Msa0531890:five_prime_UTR
TATGTGAAATGATGAGCCGG+AGG 0.656757 4_1:+5987738 Msa0531890:CDS
CACTCATATGGAGAATATAG+CGG 0.659666 4_1:+5988797 Msa0531890:CDS
CCTTCAATGCATAGAAACAG+CGG 0.663560 4_1:-5992623 None:intergenic
ACGAGAGAGAAGGAACTCAA+CGG 0.674648 4_1:+5986908 Msa0531890:five_prime_UTR
ATGTGAAATGATGAGCCGGA+GGG 0.678863 4_1:+5987739 Msa0531890:CDS
AATGAAATGAATGAAGACCA+AGG 0.691600 4_1:+5987062 Msa0531890:exon
CATTTGTAAAGTCTCCGGCA+GGG 0.692662 4_1:-5992172 None:intergenic
AAAACTCATTGATAACCTGG+AGG 0.707690 4_1:+5992032 Msa0531890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTATTTTTAATTTTT+TGG + chr4_1:5990794-5990813 Msa0531890:intron 0.0%
!!! AATTTTATTTTTAATTTTTT+GGG + chr4_1:5990795-5990814 Msa0531890:intron 0.0%
!!! ATGTTTGTTTATTTTTAAAA+GGG + chr4_1:5987398-5987417 Msa0531890:intron 10.0%
!!! AAATAATGCTTTTATTTTTT+AGG + chr4_1:5990436-5990455 Msa0531890:intron 10.0%
!! CTTAAATTTATAATCTAAAA+GGG - chr4_1:5991749-5991768 None:intergenic 10.0%
!! ATCATATTAAAAAAATTACA+AGG - chr4_1:5992476-5992495 None:intergenic 10.0%
!!! GATGTTTGTTTATTTTTAAA+AGG + chr4_1:5987397-5987416 Msa0531890:intron 15.0%
!!! TTTCAATTGCAAAATATTTT+TGG + chr4_1:5987899-5987918 Msa0531890:intron 15.0%
!!! CTATTCTTTTTAAAATCAAT+TGG + chr4_1:5988495-5988514 Msa0531890:intron 15.0%
!! CTATTTACATGTTTATATAA+TGG + chr4_1:5988718-5988737 Msa0531890:intron 15.0%
!! TATAGGAAAAATAAAATAGA+GGG - chr4_1:5988998-5989017 None:intergenic 15.0%
!! ATATAGGAAAAATAAAATAG+AGG - chr4_1:5988999-5989018 None:intergenic 15.0%
!! ATCTATTCTTAAATCTTAAA+AGG - chr4_1:5989172-5989191 None:intergenic 15.0%
!!! AAAAAGTATTATTAGATTAG+AGG + chr4_1:5989288-5989307 Msa0531890:intron 15.0%
!! TTTATGTTTAACACTAATAA+TGG - chr4_1:5989375-5989394 None:intergenic 15.0%
!! ATAATAACAAAGAAAATCTT+GGG - chr4_1:5991068-5991087 None:intergenic 15.0%
!! AATAATAACAAAGAAAATCT+TGG - chr4_1:5991069-5991088 None:intergenic 15.0%
!! GCTTAAATTTATAATCTAAA+AGG - chr4_1:5991750-5991769 None:intergenic 15.0%
!!! TTTTGATTAAACAAAAGATG+AGG - chr4_1:5987094-5987113 None:intergenic 20.0%
!! GTTTAATCAAAAATCATGAT+CGG + chr4_1:5987102-5987121 Msa0531890:intron 20.0%
!!! TTTGTTTTTCCAGAATTTTT+TGG + chr4_1:5987185-5987204 Msa0531890:intron 20.0%
!!! GTTTTTTCGTTTTTAAGTTA+TGG + chr4_1:5987359-5987378 Msa0531890:intron 20.0%
!!! GTTATGGTTTTATTTTGAAT+TGG + chr4_1:5987375-5987394 Msa0531890:intron 20.0%
!!! ATCATTACTTTTTGTGTTTA+TGG + chr4_1:5987944-5987963 Msa0531890:intron 20.0%
!! TGTGAAGAAAAAAAATTGTT+AGG + chr4_1:5988000-5988019 Msa0531890:intron 20.0%
!! AATCCTTAAGATTCATAATA+TGG + chr4_1:5988159-5988178 Msa0531890:intron 20.0%
!! AAAACAATTAAACTTAAGCT+CGG - chr4_1:5988439-5988458 None:intergenic 20.0%
!!! AGCTTAAGTTTAATTGTTTT+TGG + chr4_1:5988439-5988458 Msa0531890:intron 20.0%
!! CTGAAAGAAGATTAATAATA+TGG - chr4_1:5988762-5988781 None:intergenic 20.0%
!! CATATTATTAATCTTCTTTC+AGG + chr4_1:5988760-5988779 Msa0531890:intron 20.0%
!! TATTAGTGTTAAACATAAAC+CGG + chr4_1:5989376-5989395 Msa0531890:intron 20.0%
!!! TATAAGTCTGAAATTTATTG+TGG + chr4_1:5989726-5989745 Msa0531890:intron 20.0%
!! AAACTAACTAAACAAAAATC+AGG - chr4_1:5989786-5989805 None:intergenic 20.0%
!! CAAATTAAATCATACAATCA+TGG - chr4_1:5990757-5990776 None:intergenic 20.0%
!! CATGATTGTATGATTTAATT+TGG + chr4_1:5990755-5990774 Msa0531890:intron 20.0%
!! ACAATTTAAATATGTCCATA+TGG + chr4_1:5990822-5990841 Msa0531890:intron 20.0%
!! AATAAGATGATAGCAATTTA+TGG + chr4_1:5990970-5990989 Msa0531890:intron 20.0%
!! ATTTATATGAGCATAAATAG+TGG - chr4_1:5991032-5991051 None:intergenic 20.0%
!!! CATGTACTTTTTAGTATATT+TGG + chr4_1:5991438-5991457 Msa0531890:intron 20.0%
!!! TACTTTTTAGTATATTTGGT+GGG + chr4_1:5991442-5991461 Msa0531890:intron 20.0%
!!! TTTTCTTAAGAAATTAACCT+GGG + chr4_1:5991649-5991668 Msa0531890:intron 20.0%
!!! GATAATATATGCATGTATTT+TGG - chr4_1:5991959-5991978 None:intergenic 20.0%
!!! ATTCACTTTTAATAAAGAGA+GGG - chr4_1:5992402-5992421 None:intergenic 20.0%
! CTGAAGTGAAAAATGAAAAT+TGG + chr4_1:5986976-5986995 Msa0531890:five_prime_UTR 25.0%
! GAAAAATGAAAATTGGAAGA+AGG + chr4_1:5986983-5987002 Msa0531890:five_prime_UTR 25.0%
!! ATTCTGGAAAAACAAAAACT+TGG - chr4_1:5987181-5987200 None:intergenic 25.0%
!!! TTTTCCAGAATTTTTTGGAT+CGG + chr4_1:5987190-5987209 Msa0531890:intron 25.0%
! ATTGTCATGACATAAAATTG+GGG - chr4_1:5987297-5987316 None:intergenic 25.0%
! GATTGTCATGACATAAAATT+GGG - chr4_1:5987298-5987317 None:intergenic 25.0%
! TGATTGTCATGACATAAAAT+TGG - chr4_1:5987299-5987318 None:intergenic 25.0%
! AACGAAAAAACACAGTAAAA+AGG - chr4_1:5987350-5987369 None:intergenic 25.0%
! AAAGTTTAAAGATGACGAAA+TGG - chr4_1:5987617-5987636 None:intergenic 25.0%
! TTCTTCACATCAGTTAAAAT+GGG - chr4_1:5987989-5988008 None:intergenic 25.0%
! TTTCTTCACATCAGTTAAAA+TGG - chr4_1:5987990-5988009 None:intergenic 25.0%
! AAGAATGTTCAAATAAGATG+AGG + chr4_1:5988057-5988076 Msa0531890:intron 25.0%
! AGAATGTTCAAATAAGATGA+GGG + chr4_1:5988058-5988077 Msa0531890:intron 25.0%
! AAGCCATATTATGAATCTTA+AGG - chr4_1:5988165-5988184 None:intergenic 25.0%
! TTCTTCCAAAAAATCTGAAT+TGG + chr4_1:5988240-5988259 Msa0531890:intron 25.0%
! GTCATCTATAAGAAAACAAT+AGG - chr4_1:5988289-5988308 None:intergenic 25.0%
!! ATTGTTTTCTTATAGATGAC+AGG + chr4_1:5988289-5988308 Msa0531890:intron 25.0%
! ATCTCAATTGTTTCATACTT+TGG + chr4_1:5988586-5988605 Msa0531890:intron 25.0%
!!! TTCTTACTTTTGTGTTATCT+TGG + chr4_1:5988612-5988631 Msa0531890:intron 25.0%
! ACTAACCAAAAAACAGAAAA+GGG + chr4_1:5988677-5988696 Msa0531890:intron 25.0%
!!! TTTTTAATTCGACATGAAAG+TGG - chr4_1:5988857-5988876 None:intergenic 25.0%
! CGAATTAAAAAACAACCATT+TGG + chr4_1:5988866-5988885 Msa0531890:CDS 25.0%
! TATGAGTAAAAGATGAAAAC+AGG + chr4_1:5989121-5989140 Msa0531890:intron 25.0%
! TCTTAAATCTTAAAAGGATG+AGG - chr4_1:5989166-5989185 None:intergenic 25.0%
! TTATTAGATTAGAGGTAGAA+TGG + chr4_1:5989296-5989315 Msa0531890:intron 25.0%
! ATTGACATTAGGAAAAGAAA+AGG + chr4_1:5989433-5989452 Msa0531890:intron 25.0%
!! TAAAGGACAATCTTTTCTTT+GGG + chr4_1:5989617-5989636 Msa0531890:intron 25.0%
! TTGAATTGTTAATTGATGCT+TGG + chr4_1:5989804-5989823 Msa0531890:intron 25.0%
!!! CTTGGTATTTTTTATATACG+TGG + chr4_1:5989822-5989841 Msa0531890:intron 25.0%
!! AGCTAGATTTTAAATCATGT+AGG - chr4_1:5990353-5990372 None:intergenic 25.0%
! GAATTATGTGTTCTATTTGT+AGG + chr4_1:5990546-5990565 Msa0531890:intron 25.0%
!!! AAACTATCTTTTAGAGCTTT+TGG + chr4_1:5990946-5990965 Msa0531890:intron 25.0%
!! TATCTTTTAGATCTGTAAGA+AGG - chr4_1:5991233-5991252 None:intergenic 25.0%
! TCTAAAAGATATACCAAAGA+AGG + chr4_1:5991242-5991261 Msa0531890:CDS 25.0%
!!! GTACTTTTTAGTATATTTGG+TGG + chr4_1:5991441-5991460 Msa0531890:intron 25.0%
!! GTTTTCTTAAGAAATTAACC+TGG + chr4_1:5991648-5991667 Msa0531890:intron 25.0%
!!! TGATATTTTATTTAGCGTGA+AGG + chr4_1:5991680-5991699 Msa0531890:intron 25.0%
!!! GATATTTTATTTAGCGTGAA+GGG + chr4_1:5991681-5991700 Msa0531890:intron 25.0%
!!! ATATTTTATTTAGCGTGAAG+GGG + chr4_1:5991682-5991701 Msa0531890:intron 25.0%
! AATACTAAATGTTAGCATAG+TGG - chr4_1:5991846-5991865 None:intergenic 25.0%
! TCTATTGAAGTTACATTTCT+TGG + chr4_1:5991875-5991894 Msa0531890:intron 25.0%
! ATTCATTGATCTACATGTAT+AGG - chr4_1:5991915-5991934 None:intergenic 25.0%
! AGTAATTAAAGAGTTGTTTG+TGG - chr4_1:5992080-5992099 None:intergenic 25.0%
!!! CATTCACTTTTAATAAAGAG+AGG - chr4_1:5992403-5992422 None:intergenic 25.0%
! CAAATATGATTGTTAGCAAA+CGG + chr4_1:5992534-5992553 Msa0531890:three_prime_UTR 25.0%
! AAATATGATTGTTAGCAAAC+GGG + chr4_1:5992535-5992554 Msa0531890:three_prime_UTR 25.0%
AATGAAATGAATGAAGACCA+AGG + chr4_1:5987062-5987081 Msa0531890:exon 30.0%
AAAAGATGAGGAAATTACCT+TGG - chr4_1:5987082-5987101 None:intergenic 30.0%
AACATTACCAACCATTAAGT+TGG + chr4_1:5987664-5987683 Msa0531890:intron 30.0%
AACATTACCAACTTAATGGT+TGG - chr4_1:5987674-5987693 None:intergenic 30.0%
! AAACCCCAATTTTCATCTTT+AGG - chr4_1:5987785-5987804 None:intergenic 30.0%
AGTGGATAACAATGTAGAAA+GGG + chr4_1:5987844-5987863 Msa0531890:CDS 30.0%
TCATTTAAGTGTTTGTGAGA+TGG + chr4_1:5988358-5988377 Msa0531890:intron 30.0%
! GACAAAGGATATTTTTCACA+AGG + chr4_1:5988380-5988399 Msa0531890:intron 30.0%
!! ATAGTTTTCACTTTTCAGTC+AGG + chr4_1:5988650-5988669 Msa0531890:intron 30.0%
CACTAACCAAAAAACAGAAA+AGG + chr4_1:5988676-5988695 Msa0531890:intron 30.0%
! AAGTGGAGAATTTTCACATT+GGG - chr4_1:5988840-5988859 None:intergenic 30.0%
! AAAGTGGAGAATTTTCACAT+TGG - chr4_1:5988841-5988860 None:intergenic 30.0%
! ATGATAGGAAAGGATTTGTT+AGG + chr4_1:5988930-5988949 Msa0531890:intron 30.0%
!!! AGTTTGAGTTTTGAGTTTGA+GGG - chr4_1:5989038-5989057 None:intergenic 30.0%
!!! TAGTTTGAGTTTTGAGTTTG+AGG - chr4_1:5989039-5989058 None:intergenic 30.0%
AACTCAAAACTCAAACTACT+TGG + chr4_1:5989041-5989060 Msa0531890:intron 30.0%
CAAACTTGGACATAGAATAA+TGG + chr4_1:5989319-5989338 Msa0531890:intron 30.0%
TAATGGCGAATCAATAATTC+TGG + chr4_1:5989336-5989355 Msa0531890:intron 30.0%
ACACTAATAATGGTACAACA+GGG - chr4_1:5989365-5989384 None:intergenic 30.0%
AACACTAATAATGGTACAAC+AGG - chr4_1:5989366-5989385 None:intergenic 30.0%
TGATATGACTCATTGACATT+AGG + chr4_1:5989422-5989441 Msa0531890:intron 30.0%
ACATTAGGAAAAGAAAAGGA+TGG + chr4_1:5989437-5989456 Msa0531890:intron 30.0%
!! TCAAGCCAAAGCTTTAAAAA+TGG + chr4_1:5989592-5989611 Msa0531890:intron 30.0%
!!! TTAGGCCATTTTTAAAGCTT+TGG - chr4_1:5989600-5989619 None:intergenic 30.0%
!! AAGCTTTAAAAATGGCCTAA+AGG + chr4_1:5989600-5989619 Msa0531890:intron 30.0%
CAAAGAAAAGATTGTCCTTT+AGG - chr4_1:5989618-5989637 None:intergenic 30.0%
! CTAAAGGACAATCTTTTCTT+TGG + chr4_1:5989616-5989635 Msa0531890:intron 30.0%
! AAGTCTGAAATTTATTGTGG+TGG + chr4_1:5989729-5989748 Msa0531890:intron 30.0%
AGAAGCAAACATGTTATTCT+TGG + chr4_1:5989860-5989879 Msa0531890:intron 30.0%
AATGCTCTTGTTCTATATGA+TGG - chr4_1:5990110-5990129 None:intergenic 30.0%
! GATCATAAAACATGTTTTGC+AGG + chr4_1:5990238-5990257 Msa0531890:intron 30.0%
!!! CTTTTATTTTTTAGGCAGCT+GGG + chr4_1:5990444-5990463 Msa0531890:intron 30.0%
CTATTGTGTCAGAAAATTGA+GGG + chr4_1:5990493-5990512 Msa0531890:intron 30.0%
TTACATGAAATGTTGCATGT+TGG + chr4_1:5990590-5990609 Msa0531890:CDS 30.0%
TCAAATTGCATTCTTCTGTT+TGG + chr4_1:5990685-5990704 Msa0531890:CDS 30.0%
CATGGATAAGAAGATAACAA+TGG - chr4_1:5990739-5990758 None:intergenic 30.0%
CTTGTGACATTGTCTTATAT+TGG + chr4_1:5991132-5991151 Msa0531890:intron 30.0%
! TGAACTCTTTAAAAGAGAAC+AGG + chr4_1:5991203-5991222 Msa0531890:intron 30.0%
TTATGATCACAAACCTTCTT+TGG - chr4_1:5991258-5991277 None:intergenic 30.0%
TCTATGATGAGATACATTTG+TGG + chr4_1:5991366-5991385 Msa0531890:intron 30.0%
CTATGATGAGATACATTTGT+GGG + chr4_1:5991367-5991386 Msa0531890:intron 30.0%
TATGATGAGATACATTTGTG+GGG + chr4_1:5991368-5991387 Msa0531890:intron 30.0%
CAATTATCTTTCAACTTCTG+TGG - chr4_1:5991417-5991436 None:intergenic 30.0%
CACAGAAGTTGAAAGATAAT+TGG + chr4_1:5991415-5991434 Msa0531890:intron 30.0%
GGTGGGAAAACATTAATAAA+AGG + chr4_1:5991459-5991478 Msa0531890:intron 30.0%
CAGCAAAAATTTGCATAACA+TGG - chr4_1:5991510-5991529 None:intergenic 30.0%
! TATTGATTACATAGCTGTTG+TGG + chr4_1:5991533-5991552 Msa0531890:intron 30.0%
! TAAGCTGAAATTCTAGAGAT+TGG - chr4_1:5991561-5991580 None:intergenic 30.0%
AATTTCAGTTCAAACTCTGA+TGG - chr4_1:5991793-5991812 None:intergenic 30.0%
! ACAAAAACTCATTGATAACC+TGG + chr4_1:5992029-5992048 Msa0531890:CDS 30.0%
AAAATTGAAACACGTGTAAC+AGG + chr4_1:5992130-5992149 Msa0531890:intron 30.0%
AAATGTTGACCATTTCAAAG+AGG + chr4_1:5992190-5992209 Msa0531890:CDS 30.0%
TCGACAACTTGTATCAATTT+CGG - chr4_1:5992257-5992276 None:intergenic 30.0%
GATTAGGAATGTCCTTAAAA+TGG - chr4_1:5992330-5992349 None:intergenic 30.0%
! TTGAATGAGAGGATCTATTT+TGG + chr4_1:5992352-5992371 Msa0531890:three_prime_UTR 30.0%
! TGAATGAGAGGATCTATTTT+GGG + chr4_1:5992353-5992372 Msa0531890:three_prime_UTR 30.0%
!! ATATGATGCCTTTTATTTGC+AGG + chr4_1:5992489-5992508 Msa0531890:three_prime_UTR 30.0%
AATATGATTGTTAGCAAACG+GGG + chr4_1:5992536-5992555 Msa0531890:three_prime_UTR 30.0%
! TTCTATACTCTTTTGCTTAG+TGG + chr4_1:5992657-5992676 Msa0531890:three_prime_UTR 30.0%
AGATCGATTGAAGCAATGAA+AGG + chr4_1:5987466-5987485 Msa0531890:intron 35.0%
! ATTCATCATGCTTTGTTGAG+TGG + chr4_1:5987539-5987558 Msa0531890:intron 35.0%
AGACAATGGATCACATTGTT+GGG - chr4_1:5987568-5987587 None:intergenic 35.0%
GTTTAAAGATGACGAAATGG+AGG - chr4_1:5987614-5987633 None:intergenic 35.0%
GGAGAACATTACCAACTTAA+TGG - chr4_1:5987678-5987697 None:intergenic 35.0%
TGGCTCCTAAAGATGAAAAT+TGG + chr4_1:5987777-5987796 Msa0531890:CDS 35.0%
GGCTCCTAAAGATGAAAATT+GGG + chr4_1:5987778-5987797 Msa0531890:CDS 35.0%
GCTCCTAAAGATGAAAATTG+GGG + chr4_1:5987779-5987798 Msa0531890:CDS 35.0%
CAGTGGATAACAATGTAGAA+AGG + chr4_1:5987843-5987862 Msa0531890:CDS 35.0%
TGTAGAAAGGGTCTATAAAG+AGG + chr4_1:5987856-5987875 Msa0531890:CDS 35.0%
! GGGTCTATAAAGAGGTATAA+TGG + chr4_1:5987864-5987883 Msa0531890:intron 35.0%
!!! CCTATCCTTTTCTAGAGTTT+GGG - chr4_1:5988111-5988130 None:intergenic 35.0%
!!! ACCTATCCTTTTCTAGAGTT+TGG - chr4_1:5988112-5988131 None:intergenic 35.0%
AGGATAGGTCAAATAAGATG+AGG + chr4_1:5988123-5988142 Msa0531890:intron 35.0%
!!! GAGCTCCAATTCAGATTTTT+TGG - chr4_1:5988248-5988267 None:intergenic 35.0%
AAAGCACACTTCTTCAAAGA+TGG - chr4_1:5988523-5988542 None:intergenic 35.0%
ACCAAAAAACAGAAAAGGGA+AGG + chr4_1:5988681-5988700 Msa0531890:intron 35.0%
CCAAAAAACAGAAAAGGGAA+GGG + chr4_1:5988682-5988701 Msa0531890:intron 35.0%
CAAAAAACAGAAAAGGGAAG+GGG + chr4_1:5988683-5988702 Msa0531890:intron 35.0%
TACCATATTTGCCACTCATA+TGG + chr4_1:5988785-5988804 Msa0531890:CDS 35.0%
CACTCATATGGAGAATATAG+CGG + chr4_1:5988797-5988816 Msa0531890:CDS 35.0%
! AAACAACCATTTGGAGTTCA+AGG + chr4_1:5988875-5988894 Msa0531890:CDS 35.0%
ATACTACCTTGAACTCCAAA+TGG - chr4_1:5988884-5988903 None:intergenic 35.0%
!! TTCATTTTGACCTGCATGAT+AGG + chr4_1:5988915-5988934 Msa0531890:intron 35.0%
GGGTATCATGCATGATATAT+AGG - chr4_1:5989015-5989034 None:intergenic 35.0%
!!! TTTGAGTTTTGAGTTTGAGG+GGG - chr4_1:5989036-5989055 None:intergenic 35.0%
!!! GTTTGAGTTTTGAGTTTGAG+GGG - chr4_1:5989037-5989056 None:intergenic 35.0%
ATCTTAAAAGGATGAGGACA+TGG - chr4_1:5989160-5989179 None:intergenic 35.0%
CGACTTGATTGGAATATTTC+AGG - chr4_1:5989527-5989546 None:intergenic 35.0%
! AATCGTATTCTCGACTTGAT+TGG - chr4_1:5989538-5989557 None:intergenic 35.0%
ATCAAGTCGAGAATACGATT+CGG + chr4_1:5989538-5989557 Msa0531890:intron 35.0%
TCCTTAAGATTCATCAAAGC+TGG - chr4_1:5989564-5989583 None:intergenic 35.0%
TTATTCTTGGAATCTCCTGT+TGG + chr4_1:5989873-5989892 Msa0531890:CDS 35.0%
TAAGAAAATCCAACTCCAAC+AGG - chr4_1:5989891-5989910 None:intergenic 35.0%
TCTTAGTTGTGCATATTCAC+TGG - chr4_1:5989925-5989944 None:intergenic 35.0%
! CTTACACTTTTCTGCACAAT+TGG + chr4_1:5990072-5990091 Msa0531890:CDS 35.0%
!!! ACAAGAGCATTTTACATAGC+AGG + chr4_1:5990119-5990138 Msa0531890:CDS 35.0%
TGAGATCAATATGAAGGGAA+GGG - chr4_1:5990312-5990331 None:intergenic 35.0%
TTGAGATCAATATGAAGGGA+AGG - chr4_1:5990313-5990332 None:intergenic 35.0%
TTCCCTTCATATTGATCTCA+AGG + chr4_1:5990312-5990331 Msa0531890:CDS 35.0%
TCCCTTCATATTGATCTCAA+GGG + chr4_1:5990313-5990332 Msa0531890:CDS 35.0%
!!! TATTGATCTCAAGGGCATTT+TGG + chr4_1:5990321-5990340 Msa0531890:CDS 35.0%
!!! GCTTTTATTTTTTAGGCAGC+TGG + chr4_1:5990443-5990462 Msa0531890:intron 35.0%
GCTATTGTGTCAGAAAATTG+AGG + chr4_1:5990492-5990511 Msa0531890:intron 35.0%
AGCAGTTTCAAAGTTGTGTT+TGG + chr4_1:5990613-5990632 Msa0531890:CDS 35.0%
ATGCAATTTGAGTATCTCCT+TGG - chr4_1:5990676-5990695 None:intergenic 35.0%
TGATAAGCAGTTATGCCATA+TGG - chr4_1:5990840-5990859 None:intergenic 35.0%
AAGAAAATCTTGGGTCTGTT+CGG - chr4_1:5991059-5991078 None:intergenic 35.0%
! AGTACACCAATGTTGTTTAC+AGG - chr4_1:5991177-5991196 None:intergenic 35.0%
GTAAACAACATTGGTGTACT+TGG + chr4_1:5991177-5991196 Msa0531890:intron 35.0%
GCATAACATGGTACAAAATG+CGG - chr4_1:5991498-5991517 None:intergenic 35.0%
TCTTAAGAAATTAACCTGGG+AGG + chr4_1:5991652-5991671 Msa0531890:intron 35.0%
CTTAAGAAATTAACCTGGGA+GGG + chr4_1:5991653-5991672 Msa0531890:intron 35.0%
TTAAGAAATTAACCTGGGAG+GGG + chr4_1:5991654-5991673 Msa0531890:intron 35.0%
! AAAACTCATTGATAACCTGG+AGG + chr4_1:5992032-5992051 Msa0531890:CDS 35.0%
! AAACTCATTGATAACCTGGA+GGG + chr4_1:5992033-5992052 Msa0531890:CDS 35.0%
GTCAACATTTGTAAAGTCTC+CGG - chr4_1:5992180-5992199 None:intergenic 35.0%
AATGGACAAGGACAACAATA+AGG + chr4_1:5992296-5992315 Msa0531890:three_prime_UTR 35.0%
! AAGGACATGGATCCATTTTA+AGG + chr4_1:5992315-5992334 Msa0531890:three_prime_UTR 35.0%
TTCCTAATCTCTTGAATGAG+AGG + chr4_1:5992341-5992360 Msa0531890:three_prime_UTR 35.0%
ATCCTCTCATTCAAGAGATT+AGG - chr4_1:5992346-5992365 None:intergenic 35.0%
TCAACGGCAAAAAAGAGGAA+AGG + chr4_1:5986924-5986943 Msa0531890:five_prime_UTR 40.0%
AGGTGAAAACGAAAGAGAGA+CGG + chr4_1:5987003-5987022 Msa0531890:five_prime_UTR 40.0%
TGTTGCTATGCATGTTCGAT+TGG + chr4_1:5987151-5987170 Msa0531890:intron 40.0%
TGCTCCGATCCAAAAAATTC+TGG - chr4_1:5987197-5987216 None:intergenic 40.0%
TGATTCAAAATCCAGAGCAG+CGG - chr4_1:5987254-5987273 None:intergenic 40.0%
!! GCTCTGGATTTTGAATCAGA+AGG + chr4_1:5987256-5987275 Msa0531890:intron 40.0%
CAGACAATGGATCACATTGT+TGG - chr4_1:5987569-5987588 None:intergenic 40.0%
ATGGAGGTAATTCAACTGGA+GGG - chr4_1:5987598-5987617 None:intergenic 40.0%
AATGGAGGTAATTCAACTGG+AGG - chr4_1:5987599-5987618 None:intergenic 40.0%
CGAAATGGAGGTAATTCAAC+TGG - chr4_1:5987602-5987621 None:intergenic 40.0%
TGCAGGTCTCTGTAGAATAT+GGG + chr4_1:5987714-5987733 Msa0531890:intron 40.0%
GGTTATGTGAAATGATGAGC+CGG + chr4_1:5987735-5987754 Msa0531890:CDS 40.0%
TTTCAACATATCAGAGCGTG+AGG + chr4_1:5987802-5987821 Msa0531890:CDS 40.0%
! ATGAGCCCAAACTCTAGAAA+AGG + chr4_1:5988103-5988122 Msa0531890:intron 40.0%
! CCCAAACTCTAGAAAAGGAT+AGG + chr4_1:5988108-5988127 Msa0531890:intron 40.0%
AGTGTTTGTGAGATGGACAA+AGG + chr4_1:5988365-5988384 Msa0531890:intron 40.0%
! CTTTTGTGTTATCTTGGCCA+TGG + chr4_1:5988618-5988637 Msa0531890:intron 40.0%
AAAACTATCACCATGCTCCA+TGG - chr4_1:5988638-5988657 None:intergenic 40.0%
!! CCCTTCCCTTTTCTGTTTTT+TGG - chr4_1:5988685-5988704 None:intergenic 40.0%
CTCCATATGAGTGGCAAATA+TGG - chr4_1:5988790-5988809 None:intergenic 40.0%
CGCTATATTCTCCATATGAG+TGG - chr4_1:5988799-5988818 None:intergenic 40.0%
TTTGACCTGCATGATAGGAA+AGG + chr4_1:5988920-5988939 Msa0531890:intron 40.0%
CAAATCCTTTCCTATCATGC+AGG - chr4_1:5988928-5988947 None:intergenic 40.0%
GAAGAATGTATCAGCTGTGT+TGG + chr4_1:5988966-5988985 Msa0531890:intron 40.0%
!!! TTGAGTTTTGAGTTTGAGGG+GGG - chr4_1:5989035-5989054 None:intergenic 40.0%
TGCTTACATGACATCACCTT+AGG + chr4_1:5989205-5989224 Msa0531890:intron 40.0%
AGAAAGTTCGCAGAAGATCA+AGG - chr4_1:5989257-5989276 None:intergenic 40.0%
TTGATCTTCTGCGAACTTTC+TGG + chr4_1:5989256-5989275 Msa0531890:intron 40.0%
! TGCGAACTTTCTGGTGAAAT+AGG + chr4_1:5989265-5989284 Msa0531890:intron 40.0%
TAGAGGTAGAATGGCAAACT+TGG + chr4_1:5989305-5989324 Msa0531890:intron 40.0%
GTGCTTATCATGTTGCACAA+AGG + chr4_1:5989493-5989512 Msa0531890:intron 40.0%
! GCCAGCTTTGATGAATCTTA+AGG + chr4_1:5989560-5989579 Msa0531890:intron 40.0%
TTATTGTGGTGGTCTTTGAG+AGG + chr4_1:5989740-5989759 Msa0531890:intron 40.0%
CAACTAAGAGATGACTTCAC+AGG + chr4_1:5989936-5989955 Msa0531890:CDS 40.0%
GCCCTTGAGATCAATATGAA+GGG - chr4_1:5990317-5990336 None:intergenic 40.0%
TGCCCTTGAGATCAATATGA+AGG - chr4_1:5990318-5990337 None:intergenic 40.0%
TCTCATGTTGCAAGTGATAG+AGG + chr4_1:5990518-5990537 Msa0531890:intron 40.0%
AGTAATCGCAAGACAAGAGA+TGG + chr4_1:5990650-5990669 Msa0531890:CDS 40.0%
ACATGGTACAAAATGCGGTA+AGG - chr4_1:5991493-5991512 None:intergenic 40.0%
TAAGAAATTAACCTGGGAGG+GGG + chr4_1:5991655-5991674 Msa0531890:intron 40.0%
GCTTGGCATGTAATCTAAAG+AGG - chr4_1:5991818-5991837 None:intergenic 40.0%
TTAGCATAGTGGTAACTGCT+TGG - chr4_1:5991835-5991854 None:intergenic 40.0%
!!! TATGCATGTATTTTGGCAGC+TGG - chr4_1:5991952-5991971 None:intergenic 40.0%
AAGCAAATGCCTATCGTGAT+AGG + chr4_1:5991991-5992010 Msa0531890:CDS 40.0%
AGCAAATGCCTATCGTGATA+GGG + chr4_1:5991992-5992011 Msa0531890:CDS 40.0%
! TTAGCTTTCCCTATCACGAT+AGG - chr4_1:5992003-5992022 None:intergenic 40.0%
! AACTCATTGATAACCTGGAG+GGG + chr4_1:5992034-5992053 Msa0531890:CDS 40.0%
AACACGTGTAACAGGTACAA+AGG + chr4_1:5992138-5992157 Msa0531890:intron 40.0%
ACACGTGTAACAGGTACAAA+GGG + chr4_1:5992139-5992158 Msa0531890:intron 40.0%
! CACTCAACACCTCTTTGAAA+TGG - chr4_1:5992202-5992221 None:intergenic 40.0%
CAATGAGTTTGCAAGAGCAA+TGG + chr4_1:5992278-5992297 Msa0531890:exon 40.0%
CAAGGACAACAATAAGGACA+TGG + chr4_1:5992302-5992321 Msa0531890:three_prime_UTR 40.0%
CCTTCAATGCATAGAAACAG+CGG - chr4_1:5992626-5992645 None:intergenic 40.0%
ACGAGAGAGAAGGAACTCAA+CGG + chr4_1:5986908-5986927 Msa0531890:five_prime_UTR 45.0%
GGAACTCAACGGCAAAAAAG+AGG + chr4_1:5986919-5986938 Msa0531890:five_prime_UTR 45.0%
ACGGCAAAAAAGAGGAAAGG+AGG + chr4_1:5986927-5986946 Msa0531890:five_prime_UTR 45.0%
GCTGGAGCAGAAAAGGAAAA+CGG + chr4_1:5986953-5986972 Msa0531890:five_prime_UTR 45.0%
!! AGGAGAGGAAAGTGTTGTGT+TGG + chr4_1:5987030-5987049 Msa0531890:five_prime_UTR 45.0%
TTGGATCGGAGCAACTTCAA+CGG + chr4_1:5987204-5987223 Msa0531890:intron 45.0%
TGGAGGGATATCACAGACAA+TGG - chr4_1:5987582-5987601 None:intergenic 45.0%
CTGCAGGTCTCTGTAGAATA+TGG + chr4_1:5987713-5987732 Msa0531890:intron 45.0%
TATGTGAAATGATGAGCCGG+AGG + chr4_1:5987738-5987757 Msa0531890:CDS 45.0%
ATGTGAAATGATGAGCCGGA+GGG + chr4_1:5987739-5987758 Msa0531890:CDS 45.0%
AGAAAAGGGAAGGGGTGAAA+AGG + chr4_1:5988691-5988710 Msa0531890:intron 45.0%
AGGATGAGGACATGGAACAA+TGG - chr4_1:5989152-5989171 None:intergenic 45.0%
TTAGTGTCTGTGCTTAGTGC+CGG - chr4_1:5989398-5989417 None:intergenic 45.0%
TTGGAATCTCCTGTTGGAGT+TGG + chr4_1:5989879-5989898 Msa0531890:CDS 45.0%
! GTTTACAGGCTGGTCATTGA+AGG - chr4_1:5991163-5991182 None:intergenic 45.0%
GACCAGCCTGTAAACAACAT+TGG + chr4_1:5991168-5991187 Msa0531890:intron 45.0%
! CACCAATGTTGTTTACAGGC+TGG - chr4_1:5991173-5991192 None:intergenic 45.0%
AAAATATCATTCCCCCTCCC+AGG - chr4_1:5991669-5991688 None:intergenic 45.0%
! ACTCATTGATAACCTGGAGG+GGG + chr4_1:5992035-5992054 Msa0531890:CDS 45.0%
CATTTGTAAAGTCTCCGGCA+GGG - chr4_1:5992175-5992194 None:intergenic 45.0%
ACATTTGTAAAGTCTCCGGC+AGG - chr4_1:5992176-5992195 None:intergenic 45.0%
GTTTGCAAGAGCAATGGACA+AGG + chr4_1:5992284-5992303 Msa0531890:three_prime_UTR 45.0%
!! GCCTTTTATTTGCAGGTACG+TGG + chr4_1:5992496-5992515 Msa0531890:three_prime_UTR 45.0%
GCCACGTACCTGCAAATAAA+AGG - chr4_1:5992500-5992519 None:intergenic 45.0%
!! CCGCTGTTTCTATGCATTGA+AGG + chr4_1:5992623-5992642 Msa0531890:three_prime_UTR 45.0%
!! TATTTCATTTATTTATAATA+TGG + chr4_1:5988216-5988235 Msa0531890:intron 5.0%
AAAGAGGAAAGGAGGAACGC+TGG + chr4_1:5986935-5986954 Msa0531890:five_prime_UTR 50.0%
CTGCAGTTGTGGAAGAGAGT+TGG - chr4_1:5987699-5987718 None:intergenic 50.0%
CAACTCTCTTCCACAACTGC+AGG + chr4_1:5987697-5987716 Msa0531890:intron 50.0%
TCTACAGAGACCTGCAGTTG+TGG - chr4_1:5987710-5987729 None:intergenic 50.0%
TTGTTATCCACTGTCCCTGC+AGG - chr4_1:5987836-5987855 None:intergenic 50.0%
GTTATCTTGGCCATGGAGCA+TGG + chr4_1:5988625-5988644 Msa0531890:intron 50.0%
CCTCGCTATGCAGAATCCTA+AGG - chr4_1:5989224-5989243 None:intergenic 50.0%
CCTTAGGATTCTGCATAGCG+AGG + chr4_1:5989221-5989240 Msa0531890:intron 50.0%
ATAGCAGGAGAGAGCTATGC+AGG + chr4_1:5990134-5990153 Msa0531890:CDS 50.0%
CCAATCCTGCTCACTCTTCA+GGG - chr4_1:5990473-5990492 None:intergenic 50.0%
GAGGAACGCTGGAGCAGAAA+AGG + chr4_1:5986946-5986965 Msa0531890:five_prime_UTR 55.0%
AACGAAAGAGAGACGGCGAG+AGG + chr4_1:5987010-5987029 Msa0531890:five_prime_UTR 55.0%
AGAGCAGCGGAAACGATTGC+CGG - chr4_1:5987241-5987260 None:intergenic 55.0%
TGATGAGCCGGAGGGCAATT+AGG + chr4_1:5987747-5987766 Msa0531890:CDS 55.0%
CCTCAAGCCTAATTGCCCTC+CGG - chr4_1:5987757-5987776 None:intergenic 55.0%
GAGGGCAATTAGGCTTGAGG+TGG + chr4_1:5987757-5987776 Msa0531890:CDS 55.0%
GGAAACCCTGAAGAGTGAGC+AGG + chr4_1:5990465-5990484 Msa0531890:intron 55.0%
CCCTGAAGAGTGAGCAGGAT+TGG + chr4_1:5990470-5990489 Msa0531890:intron 55.0%
GCCAATCCTGCTCACTCTTC+AGG - chr4_1:5990474-5990493 None:intergenic 55.0%
CGCAAGACAAGAGATGGCCA+AGG + chr4_1:5990656-5990675 Msa0531890:CDS 55.0%
TAAAGTCTCCGGCAGGGAGA+TGG - chr4_1:5992169-5992188 None:intergenic 55.0%
AAGAGAGACGGCGAGAGGAG+AGG + chr4_1:5987015-5987034 Msa0531890:five_prime_UTR 60.0%
GGAGCAACTTCAACGGCGAC+CGG + chr4_1:5987211-5987230 Msa0531890:intron 60.0%
GGAAACGATTGCCGGTGCAC+CGG - chr4_1:5987233-5987252 None:intergenic 60.0%
GGCAATCGTTTCCGCTGCTC+TGG + chr4_1:5987240-5987259 Msa0531890:intron 60.0%
CCGGAGGGCAATTAGGCTTG+AGG + chr4_1:5987754-5987773 Msa0531890:CDS 60.0%
GTGAGGCCATGCTTCCTGCA+GGG + chr4_1:5987819-5987838 Msa0531890:CDS 60.0%
CACTGTCCCTGCAGGAAGCA+TGG - chr4_1:5987828-5987847 None:intergenic 60.0%
CATGCTTCCTGCAGGGACAG+TGG + chr4_1:5987826-5987845 Msa0531890:CDS 60.0%
CCTTCGTGAATTCCCCCTCC+AGG - chr4_1:5992050-5992069 None:intergenic 60.0%
CCTGGAGGGGGAATTCACGA+AGG + chr4_1:5992047-5992066 Msa0531890:CDS 60.0%
AGGGAGTTCCATCTCCCTGC+CGG + chr4_1:5992158-5992177 Msa0531890:CDS 60.0%
CAGGTACGTGGCAAGCGAGT+TGG + chr4_1:5992508-5992527 Msa0531890:three_prime_UTR 60.0%
TTCAACGGCGACCGGTGCAC+CGG + chr4_1:5987219-5987238 Msa0531890:intron 65.0%
CGTGAGGCCATGCTTCCTGC+AGG + chr4_1:5987818-5987837 Msa0531890:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 5986903 5992731 5986903 ID=Msa0531890;Name=Msa0531890
chr4_1 mRNA 5986903 5992731 5986903 ID=Msa0531890-mRNA-1;Parent=Msa0531890;Name=Msa0531890-mRNA-1;_AED=0.29;_eAED=0.29;_QI=169|0.4|0.72|1|0.8|0.81|11|448|349
chr4_1 exon 5986903 5987083 5986903 ID=Msa0531890-mRNA-1:exon:2041;Parent=Msa0531890-mRNA-1
chr4_1 exon 5987719 5987877 5987719 ID=Msa0531890-mRNA-1:exon:2042;Parent=Msa0531890-mRNA-1
chr4_1 exon 5988782 5988896 5988782 ID=Msa0531890-mRNA-1:exon:2043;Parent=Msa0531890-mRNA-1
chr4_1 exon 5989862 5989957 5989862 ID=Msa0531890-mRNA-1:exon:2044;Parent=Msa0531890-mRNA-1
chr4_1 exon 5990063 5990155 5990063 ID=Msa0531890-mRNA-1:exon:2045;Parent=Msa0531890-mRNA-1
chr4_1 exon 5990260 5990342 5990260 ID=Msa0531890-mRNA-1:exon:2046;Parent=Msa0531890-mRNA-1
chr4_1 exon 5990462 5990478 5990462 ID=Msa0531890-mRNA-1:exon:2047;Parent=Msa0531890-mRNA-1
chr4_1 exon 5990568 5990720 5990568 ID=Msa0531890-mRNA-1:exon:2048;Parent=Msa0531890-mRNA-1
chr4_1 exon 5991218 5991263 5991218 ID=Msa0531890-mRNA-1:exon:2049;Parent=Msa0531890-mRNA-1
chr4_1 exon 5991925 5992068 5991925 ID=Msa0531890-mRNA-1:exon:2050;Parent=Msa0531890-mRNA-1
chr4_1 exon 5992152 5992731 5992152 ID=Msa0531890-mRNA-1:exon:2051;Parent=Msa0531890-mRNA-1
chr4_1 five_prime_UTR 5986903 5987071 5986903 ID=Msa0531890-mRNA-1:five_prime_utr;Parent=Msa0531890-mRNA-1
chr4_1 CDS 5987072 5987083 5987072 ID=Msa0531890-mRNA-1:cds;Parent=Msa0531890-mRNA-1
chr4_1 CDS 5987719 5987877 5987719 ID=Msa0531890-mRNA-1:cds;Parent=Msa0531890-mRNA-1
chr4_1 CDS 5988782 5988896 5988782 ID=Msa0531890-mRNA-1:cds;Parent=Msa0531890-mRNA-1
chr4_1 CDS 5989862 5989957 5989862 ID=Msa0531890-mRNA-1:cds;Parent=Msa0531890-mRNA-1
chr4_1 CDS 5990063 5990155 5990063 ID=Msa0531890-mRNA-1:cds;Parent=Msa0531890-mRNA-1
chr4_1 CDS 5990260 5990342 5990260 ID=Msa0531890-mRNA-1:cds;Parent=Msa0531890-mRNA-1
chr4_1 CDS 5990462 5990478 5990462 ID=Msa0531890-mRNA-1:cds;Parent=Msa0531890-mRNA-1
chr4_1 CDS 5990568 5990720 5990568 ID=Msa0531890-mRNA-1:cds;Parent=Msa0531890-mRNA-1
chr4_1 CDS 5991218 5991263 5991218 ID=Msa0531890-mRNA-1:cds;Parent=Msa0531890-mRNA-1
chr4_1 CDS 5991925 5992068 5991925 ID=Msa0531890-mRNA-1:cds;Parent=Msa0531890-mRNA-1
chr4_1 CDS 5992152 5992283 5992152 ID=Msa0531890-mRNA-1:cds;Parent=Msa0531890-mRNA-1
chr4_1 three_prime_UTR 5992284 5992731 5992284 ID=Msa0531890-mRNA-1:three_prime_utr;Parent=Msa0531890-mRNA-1
Gene Sequence

>Msa0531890

ATGAAGACCAAGGTCTCTGTAGAATATGGGTTATGTGAAATGATGAGCCGGAGGGCAATTAGGCTTGAGGTGGCTCCTAAAGATGAAAATTGGGGTTTCAACATATCAGAGCGTGAGGCCATGCTTCCTGCAGGGACAGTGGATAACAATGTAGAAAGGGTCTATAAAGAGGTATACCATATTTGCCACTCATATGGAGAATATAGCGGAATTAGCAACAATCTATCCCAATGTGAAAATTCTCCACTTTCATGTCGAATTAAAAAACAACCATTTGGAGTTCAAGAAGCAAACATGTTATTCTTGGAATCTCCTGTTGGAGTTGGATTTTCTTACTCAAATACAACCAGTGAATATGCACAACTAAGAGATGACTTCACAGCTAATGATGCTTACACTTTTCTGCACAATTGGTTTCTCAAGTATCCATCATATAGAACAAGAGCATTTTACATAGCAGGAGAGAGCTATGCAGGAAAGTACGTACTAGAGCTTGCTGAACTCATCATTGATAGAAACAATAACCCTTCCCTTCATATTGATCTCAAGGGCATTTTGCTGGGAAACCCTGAAGAGTATTCTGCAAATTCTAAAGTTTTACATGAAATGTTGCATGTTGGAGCAGTTTCAAAGTTGTGTTTGGTGCTTCAAGTTGATAGTAATCGCAAGACAAGAGATGGCCAAGGAGATACTCAAATTGCATTCTTCTGTTTGGAAGAATTCTACATAGAACAGGAAGACCTTCTTACAGATCTAAAAGATATACCAAAGAAGATCAATGAATTTGTAAAACGAGCTCCAGCTGCCAAAATACATGCATATATTATCAGTCATCTGAAAAAGCAAATGCCTATCGTGATAGGGAAAGCTAAAGTTCAACAAAAACTCATTGATAACCTGGAGGGGGAATTCACGAAGGTACAAAGGGAGTTCCATCTCCCTGCCGGAGACTTTACAAATGTTGACCATTTCAAAGAGGTGTTGAGTGTTTATAACATCGACAAGTTTGAGAAATTGAAACCGAAATTGATACAAGTTGTCGATCAATGA
Protein sequence

>Msa0531890

MKTKVSVEYGLCEMMSRRAIRLEVAPKDENWGFNISEREAMLPAGTVDNNVERVYKEVYHICHSYGEYSGISNNLSQCENSPLSCRIKKQPFGVQEANMLFLESPVGVGFSYSNTTSEYAQLRDDFTANDAYTFLHNWFLKYPSYRTRAFYIAGESYAGKYVLELAELIIDRNNNPSLHIDLKGILLGNPEEYSANSKVLHEMLHVGAVSKLCLVLQVDSNRKTRDGQGDTQIAFFCLEEFYIEQEDLLTDLKDIPKKINEFVKRAPAAKIHAYIISHLKKQMPIVIGKAKVQQKLIDNLEGEFTKVQREFHLPAGDFTNVDHFKEVLSVYNIDKFEKLKPKLIQVVDQ*