AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0817220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0817220 MtrunA17_Chr2g0281621 94.845 97 5 0 95 191 122 218 8.71e-58 194
Msa0817220 MtrunA17_Chr3g0100041 86.087 115 11 1 239 348 306 420 1.02e-56 190
Msa0817220 MtrunA17_Chr3g0099991 81.739 115 16 1 239 348 339 453 4.91e-54 184
Msa0817220 MtrunA17_Chr4g0047861 83.168 101 17 0 95 195 142 242 2.31e-51 177
Msa0817220 MtrunA17_Chr5g0444771 77.391 115 21 1 239 348 410 524 8.85e-51 177
Msa0817220 MtrunA17_Chr2g0281641 80.412 97 19 0 95 191 42 138 5.95e-50 171
Msa0817220 MtrunA17_Chr4g0075521 73.043 115 26 1 239 348 409 523 1.49e-48 171
Msa0817220 MtrunA17_Chr2g0289411 71.681 113 27 1 241 348 409 521 2.90e-46 165
Msa0817220 MtrunA17_Chr4g0068861 52.336 107 49 1 87 191 163 269 1.06e-31 124
Msa0817220 MtrunA17_Chr4g0068841 55.556 108 45 2 87 191 117 224 1.19e-31 124
Msa0817220 MtrunA17_Chr4g0038311 48.837 129 60 3 64 189 93 218 3.08e-31 122
Msa0817220 MtrunA17_Chr7g0221551 52.586 116 50 2 79 190 95 209 2.52e-29 117
Msa0817220 MtrunA17_Chr3g0118841 57.009 107 40 3 87 189 157 261 2.64e-28 115
Msa0817220 MtrunA17_Chr8g0334871 52.991 117 36 3 2 114 122 223 8.60e-28 109
Msa0817220 MtrunA17_Chr3g0118891 54.286 105 46 2 87 189 156 260 7.48e-27 110
Msa0817220 MtrunA17_Chr3g0118831 54.206 107 43 3 87 189 156 260 1.60e-26 109
Msa0817220 MtrunA17_Chr1g0199991 56.250 96 37 2 97 189 124 217 1.96e-26 109
Msa0817220 MtrunA17_Chr1g0165811 39.355 155 82 4 82 228 101 251 2.11e-26 105
Msa0817220 MtrunA17_Chr4g0020241 56.989 93 40 0 97 189 162 254 2.96e-26 108
Msa0817220 MtrunA17_Chr1g0153931 56.044 91 38 1 97 185 165 255 2.99e-26 105
Msa0817220 MtrunA17_Chr3g0118881 58.947 95 36 2 97 189 167 260 3.09e-26 108
Msa0817220 MtrunA17_Chr3g0118851 55.140 107 42 3 87 189 156 260 3.21e-26 108
Msa0817220 MtrunA17_Chr3g0118861 61.957 92 30 2 97 185 165 254 5.25e-26 108
Msa0817220 MtrunA17_Chr1g0199971 54.167 96 39 2 97 189 124 217 3.40e-25 105
Msa0817220 MtrunA17_Chr5g0405131 85.714 56 8 0 2 57 8 63 4.32e-25 99.8
Msa0817220 MtrunA17_Chr5g0405131 91.176 34 3 0 161 194 134 167 3.19e-13 67.0
Msa0817220 MtrunA17_Chr1g0153881 52.632 95 43 1 97 189 179 273 6.02e-25 105
Msa0817220 MtrunA17_Chr3g0090331 56.383 94 40 1 97 189 163 256 1.10e-24 104
Msa0817220 MtrunA17_Chr3g0126051 29.365 252 154 7 95 330 120 363 1.45e-24 103
Msa0817220 MtrunA17_Chr5g0431301 49.485 97 47 2 95 190 116 211 4.34e-24 102
Msa0817220 MtrunA17_Chr2g0304931 52.128 94 41 1 97 190 127 216 1.12e-23 101
Msa0817220 MtrunA17_Chr3g0131731 52.577 97 44 2 95 190 116 211 1.30e-23 101
Msa0817220 MtrunA17_Chr3g0126031 49.474 95 46 2 97 190 117 210 2.30e-23 100
Msa0817220 MtrunA17_Chr3g0094811 51.042 96 43 1 95 190 115 206 3.92e-23 99.8
Msa0817220 MtrunA17_Chr1g0153941 52.632 95 43 1 97 189 167 261 1.28e-22 98.6
Msa0817220 MtrunA17_Chr8g0385871 44.828 116 59 2 79 190 98 212 2.07e-22 97.8
Msa0817220 MtrunA17_Chr7g0219051 49.462 93 47 0 97 189 124 216 2.59e-21 94.7
Msa0817220 MtrunA17_Chr7g0232961 57.500 80 30 1 1 76 141 220 5.03e-20 89.0
Msa0817220 MtrunA17_Chr2g0318911 45.833 96 51 1 95 189 129 224 1.03e-19 90.1
Msa0817220 MtrunA17_Chr2g0309521 45.833 96 51 1 95 189 128 223 6.59e-19 87.4
Msa0817220 MtrunA17_Chr2g0309511 40.336 119 68 2 95 210 128 246 7.37e-19 87.4
Msa0817220 MtrunA17_Chr7g0232491 62.121 66 25 0 2 67 114 179 1.77e-18 84.3
Msa0817220 MtrunA17_Chr2g0309541 44.444 99 50 3 95 189 127 224 8.73e-18 84.3
Msa0817220 MtrunA17_Chr7g0249431 41.026 117 59 3 74 189 108 215 9.58e-17 81.3
Msa0817220 MtrunA17_Chr7g0234051 46.392 97 46 3 97 190 123 216 5.02e-16 79.0
Msa0817220 MtrunA17_Chr4g0028241 40.625 96 55 2 95 189 160 254 1.03e-15 78.2
Msa0817220 MtrunA17_Chr7g0234041 43.119 109 49 5 86 190 108 207 7.49e-15 75.5
Msa0817220 MtrunA17_Chr2g0327381 37.500 96 59 1 95 189 166 261 8.47e-15 75.5
Msa0817220 MtrunA17_Chr3g0093971 34.400 125 66 5 79 190 91 212 2.71e-14 73.6
Msa0817220 MtrunA17_Chr1g0171411 40.426 94 54 2 97 189 156 248 4.77e-14 73.2
Msa0817220 MtrunA17_Chr1g0199171 37.398 123 60 5 71 189 145 254 5.18e-14 72.8
Msa0817220 MtrunA17_Chr8g0338461 44.792 96 47 4 97 189 124 216 5.35e-14 72.8
Msa0817220 MtrunA17_Chr2g0327401 38.298 94 56 2 97 189 174 266 1.04e-13 72.0
Msa0817220 MtrunA17_Chr4g0028261 38.542 96 57 2 95 189 189 283 5.04e-13 70.1
Msa0817220 MtrunA17_Chr4g0028271 36.364 121 64 4 71 189 153 262 8.75e-13 69.3
Msa0817220 MtrunA17_Chr4g0028281 37.398 123 60 5 71 189 151 260 8.84e-13 69.3
Msa0817220 MtrunA17_Chr2g0322021 49.333 75 26 2 153 226 312 375 8.03e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0817220 AT3G20290.3 77.391 115 21 1 239 348 412 526 3.48e-50 176
Msa0817220 AT3G20290.1 77.391 115 21 1 239 348 412 526 3.48e-50 176
Msa0817220 AT3G20290.2 77.391 115 21 1 239 348 412 526 3.48e-50 176
Msa0817220 AT4G05520.1 70.435 115 29 1 239 348 412 526 1.32e-45 163
Msa0817220 AT4G05520.2 70.435 115 29 1 239 348 411 525 1.37e-45 163
Msa0817220 AT1G61130.1 65.741 108 33 2 87 190 109 216 1.84e-39 145
Msa0817220 AT1G11080.2 67.925 106 29 1 95 195 137 242 6.78e-38 142
Msa0817220 AT1G11080.1 67.925 106 29 1 95 195 137 242 6.79e-38 142
Msa0817220 AT4G15100.1 54.135 133 46 3 95 213 131 262 1.10e-35 135
Msa0817220 AT3G17180.2 60.606 99 37 1 94 190 126 224 1.39e-31 124
Msa0817220 AT3G17180.1 60.606 99 37 1 94 190 126 224 2.41e-31 123
Msa0817220 AT2G35770.1 51.261 119 51 3 97 210 127 243 4.00e-29 117
Msa0817220 AT5G23210.4 53.271 107 47 2 86 189 33 139 1.69e-28 114
Msa0817220 AT5G08260.1 55.670 97 41 1 95 189 127 223 2.29e-28 115
Msa0817220 AT2G33530.1 50.862 116 52 2 79 190 98 212 2.50e-28 115
Msa0817220 AT5G23210.2 53.271 107 47 2 86 189 33 139 4.10e-28 113
Msa0817220 AT5G23210.5 53.271 107 47 2 86 189 34 140 4.19e-28 113
Msa0817220 AT5G23210.3 53.271 107 47 2 86 189 129 235 7.56e-28 113
Msa0817220 AT1G28110.1 50.862 116 52 2 79 190 96 210 9.10e-28 113
Msa0817220 AT1G28110.2 50.862 116 52 2 79 190 96 210 9.10e-28 113
Msa0817220 AT5G23210.1 53.271 107 47 2 86 189 129 235 1.17e-27 113
Msa0817220 AT3G07990.1 56.250 96 37 2 97 189 125 218 6.76e-26 108
Msa0817220 AT2G24000.1 55.556 99 39 2 96 191 119 215 1.13e-25 107
Msa0817220 AT2G35780.1 55.914 93 38 2 97 189 121 210 3.21e-25 106
Msa0817220 AT3G52000.1 55.208 96 40 2 96 191 151 243 1.77e-24 104
Msa0817220 AT3G52020.1 55.319 94 41 1 97 190 167 259 2.09e-24 104
Msa0817220 AT2G05850.1 48.980 98 45 2 96 190 152 247 7.02e-23 99.8
Msa0817220 AT3G63470.1 54.839 93 39 1 97 189 170 259 1.06e-22 99.4
Msa0817220 AT3G52010.1 52.041 98 42 2 96 190 152 247 1.23e-22 99.0
Msa0817220 AT4G30610.1 46.552 116 58 2 95 206 120 235 3.70e-22 97.4
Msa0817220 AT1G43780.1 47.959 98 48 2 95 190 124 220 6.66e-22 96.7
Msa0817220 AT4G30810.1 48.958 96 48 1 95 189 125 220 7.90e-22 96.7
Msa0817220 AT2G24010.4 50.000 96 46 1 96 189 104 199 1.80e-20 91.7
Msa0817220 AT2G24010.2 50.000 96 46 1 96 189 133 228 2.98e-20 92.0
Msa0817220 AT2G24010.1 50.000 96 46 1 96 189 118 213 3.35e-20 91.7
Msa0817220 AT2G12480.4 45.361 97 51 2 95 190 118 213 4.43e-20 91.3
Msa0817220 AT2G12480.1 45.361 97 51 2 95 190 118 213 4.51e-20 91.3
Msa0817220 AT2G12480.2 45.361 97 51 2 95 190 118 213 4.54e-20 91.3
Msa0817220 AT3G02110.1 47.312 93 49 0 97 189 126 218 4.63e-20 91.3
Msa0817220 AT2G12480.3 45.361 97 51 2 95 190 118 213 4.75e-20 91.3
Msa0817220 AT5G42240.1 44.330 97 52 2 95 190 119 214 1.06e-19 90.5
Msa0817220 AT2G24010.3 48.936 94 46 1 96 187 104 197 1.48e-18 87.0
Msa0817220 AT3G56540.1 60.606 66 26 0 97 162 167 232 2.53e-18 84.0
Msa0817220 AT4G12910.2 46.809 94 48 2 97 189 128 220 4.83e-18 85.5
Msa0817220 AT4G12910.1 46.809 94 48 2 97 189 128 220 6.16e-18 85.1
Msa0817220 AT3G25420.1 46.809 94 48 2 97 189 121 213 8.77e-18 84.7
Msa0817220 AT5G42230.1 42.268 97 54 2 95 190 115 210 9.11e-18 84.7
Msa0817220 AT5G42230.2 42.268 97 54 2 95 190 115 210 9.11e-18 84.7
Msa0817220 AT3G45010.1 39.496 119 61 5 79 189 150 265 1.94e-15 77.8
Msa0817220 AT3G10410.1 41.837 98 51 3 95 189 169 263 2.18e-14 74.7
Msa0817220 AT3G60450.1 37.815 119 51 2 2 114 110 211 1.20e-13 70.9
Msa0817220 AT3G60450.2 37.815 119 51 2 2 114 142 243 1.31e-13 70.9
Msa0817220 AT3G60420.1 38.583 127 66 3 2 128 124 238 1.84e-13 70.1
Msa0817220 AT2G23010.1 39.000 100 54 3 94 190 115 210 1.29e-12 68.9
Msa0817220 AT2G23010.2 39.000 100 54 3 94 190 115 210 1.36e-12 68.9
Msa0817220 AT1G73290.2 37.755 98 59 2 94 190 122 218 2.11e-12 67.4
Msa0817220 AT5G22980.1 34.959 123 63 5 97 205 170 289 3.48e-12 67.8
Msa0817220 AT3G60440.2 54.386 57 26 0 2 58 133 189 4.66e-12 65.9
Msa0817220 AT1G33540.2 37.895 95 57 2 97 190 79 172 4.84e-12 67.0
Msa0817220 AT1G33540.1 37.895 95 57 2 97 190 122 215 5.53e-12 67.0
Msa0817220 AT2G23000.1 36.893 103 62 2 97 198 118 218 8.83e-12 66.2
Msa0817220 AT2G23000.2 36.893 103 62 2 97 198 118 218 8.83e-12 66.2
Msa0817220 AT1G73290.3 37.755 98 59 2 94 190 122 218 9.74e-12 66.2
Msa0817220 AT3G60440.1 54.386 57 26 0 2 58 133 189 1.19e-11 65.1
Msa0817220 AT1G73290.1 37.755 98 59 2 94 190 122 218 1.23e-11 65.9
Msa0817220 AT2G22980.3 38.144 97 55 2 94 190 118 209 1.37e-11 65.5
Msa0817220 AT3G60415.1 56.140 57 25 0 2 58 109 165 1.38e-11 64.7
Msa0817220 AT1G73300.1 37.000 100 57 3 94 190 123 219 3.79e-11 64.3
Msa0817220 AT1G73300.2 37.000 100 57 3 94 190 123 219 3.79e-11 64.3
Msa0817220 AT2G22980.5 38.298 94 53 2 97 190 2 90 4.14e-11 63.5
Msa0817220 AT2G22980.4 38.298 94 53 2 97 190 119 207 4.71e-11 64.3
Msa0817220 AT2G22980.1 38.298 94 53 2 97 190 119 207 6.30e-11 63.5
Msa0817220 AT2G22980.2 38.298 94 53 2 97 190 119 207 7.21e-11 63.5

Find 97 sgRNAs with CRISPR-Local

Find 325 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAATGAGAGGATCTATTT+TGG 0.177743 5_3:-52174665 Msa0817220:three_prime_UTR
GATAATATATGCATGTATTT+TGG 0.206206 5_3:+52175061 None:intergenic
TATTGATCTCAAGGGCATTT+TGG 0.225051 5_3:-52176696 Msa0817220:intron
CTGCAGGTCTCTGTAGAATA+TGG 0.240516 5_3:-52179304 Msa0817220:intron
GCCAATCCTGCTCACTCTTC+AGG 0.248426 5_3:+52176546 None:intergenic
TGGCTCCTAAAGATGAAAAT+TGG 0.260885 5_3:-52179240 Msa0817220:CDS
TTATGATCACAAACCTTCTT+TGG 0.264058 5_3:+52175762 None:intergenic
CTCCATATGAGTGGCAAATA+TGG 0.266024 5_3:+52178230 None:intergenic
GATTAGGAATGTCCTTAAAA+TGG 0.291784 5_3:+52174690 None:intergenic
GCCACGTACCTGCAAATAAA+AGG 0.291918 5_3:+52174520 None:intergenic
AGAAGCAAACATGTTATTCT+TGG 0.302955 5_3:-52177157 Msa0817220:CDS
TCAAATTGCATTCTTCTGTT+TGG 0.342684 5_3:-52176332 Msa0817220:CDS
TCGACAACTTGTATCAATTT+CGG 0.347320 5_3:+52174763 None:intergenic
AAGCAAATGCCTATCGTGAT+AGG 0.362210 5_3:-52175026 Msa0817220:CDS
GGCTCCTAAAGATGAAAATT+GGG 0.366205 5_3:-52179239 Msa0817220:CDS
CCGCTGTTTCTATGCATTGA+AGG 0.376433 5_3:-52174394 Msa0817220:three_prime_UTR
AAATATGATTGTTAGCAAAC+GGG 0.407617 5_3:-52174482 Msa0817220:three_prime_UTR
TTGGAATCTCCTGTTGGAGT+TGG 0.412688 5_3:-52177138 Msa0817220:CDS
TGATGAGCCGGAGGGCAATT+AGG 0.415742 5_3:-52179270 Msa0817220:CDS
AATGGACAAGGACAACAATA+AGG 0.416606 5_3:-52174721 Msa0817220:three_prime_UTR
TACCATATTTGCCACTCATA+TGG 0.416671 5_3:-52178232 Msa0817220:CDS
AAACAACCATTTGGAGTTCA+AGG 0.419670 5_3:-52178142 Msa0817220:intron
CACTCAACACCTCTTTGAAA+TGG 0.422954 5_3:+52174818 None:intergenic
CAAATATGATTGTTAGCAAA+CGG 0.429314 5_3:-52174483 Msa0817220:three_prime_UTR
AGCAGTTTCAAAGTTGTGTT+TGG 0.435209 5_3:-52176404 Msa0817220:CDS
AATGCTCTTGTTCTATATGA+TGG 0.436461 5_3:+52176910 None:intergenic
CCAATCCTGCTCACTCTTCA+GGG 0.438226 5_3:+52176547 None:intergenic
TAAGAAAATCCAACTCCAAC+AGG 0.446307 5_3:+52177129 None:intergenic
TCTTAGTTGTGCATATTCAC+TGG 0.451439 5_3:+52177095 None:intergenic
CGTGAGGCCATGCTTCCTGC+AGG 0.455082 5_3:-52179199 Msa0817220:CDS
TGAACTCTTTAAAAGAGAAC+AGG 0.466788 5_3:-52175814 Msa0817220:intron
TTATTCTTGGAATCTCCTGT+TGG 0.472255 5_3:-52177144 Msa0817220:CDS
TGCCCTTGAGATCAATATGA+AGG 0.472647 5_3:+52176702 None:intergenic
GAGGAACGCTGGAGCAGAAA+AGG 0.473807 5_3:-52180071 Msa0817220:five_prime_UTR
ATAGGAATACTATAAATCAT+AGG 0.479179 5_3:+52174307 None:intergenic
TTCCCTTCATATTGATCTCA+AGG 0.480182 5_3:-52176705 Msa0817220:CDS
TCTAAAAGATATACCAAAGA+AGG 0.481427 5_3:-52175775 Msa0817220:intron
ATACTACCTTGAACTCCAAA+TGG 0.498641 5_3:+52178136 None:intergenic
TAAAGTCTCCGGCAGGGAGA+TGG 0.499615 5_3:+52174851 None:intergenic
AAGAGAGACGGCGAGAGGAG+AGG 0.501706 5_3:-52180002 Msa0817220:five_prime_UTR
ATAGCAGGAGAGAGCTATGC+AGG 0.504990 5_3:-52176883 Msa0817220:intron
GTCAACATTTGTAAAGTCTC+CGG 0.507418 5_3:+52174840 None:intergenic
TGCAGGTCTCTGTAGAATAT+GGG 0.508561 5_3:-52179303 Msa0817220:intron
CACTGTCCCTGCAGGAAGCA+TGG 0.520311 5_3:+52179192 None:intergenic
ACATTTGTAAAGTCTCCGGC+AGG 0.522144 5_3:+52174844 None:intergenic
ATCCTCTCATTCAAGAGATT+AGG 0.524602 5_3:+52174674 None:intergenic
CAGGTACGTGGCAAGCGAGT+TGG 0.525525 5_3:-52174509 Msa0817220:three_prime_UTR
ATGCAATTTGAGTATCTCCT+TGG 0.525810 5_3:+52176344 None:intergenic
TTGTTATCCACTGTCCCTGC+AGG 0.531343 5_3:+52179184 None:intergenic
AGTGGATAACAATGTAGAAA+GGG 0.541133 5_3:-52179173 Msa0817220:CDS
ACACGTGTAACAGGTACAAA+GGG 0.542235 5_3:-52174878 Msa0817220:intron
TTAGCTTTCCCTATCACGAT+AGG 0.543279 5_3:+52175017 None:intergenic
CAGTGGATAACAATGTAGAA+AGG 0.543991 5_3:-52179174 Msa0817220:CDS
AGCAAATGCCTATCGTGATA+GGG 0.544684 5_3:-52175025 Msa0817220:CDS
AGGGAGTTCCATCTCCCTGC+CGG 0.550817 5_3:-52174859 Msa0817220:CDS
AGGAGAGGAAAGTGTTGTGT+TGG 0.553945 5_3:-52179987 Msa0817220:five_prime_UTR
TGTAGAAAGGGTCTATAAAG+AGG 0.560431 5_3:-52179161 Msa0817220:intron
TTCCTAATCTCTTGAATGAG+AGG 0.563360 5_3:-52174676 Msa0817220:three_prime_UTR
AAAGAGGAAAGGAGGAACGC+TGG 0.572269 5_3:-52180082 Msa0817220:five_prime_UTR
AAATGTTGACCATTTCAAAG+AGG 0.573061 5_3:-52174827 Msa0817220:CDS
CCTCAAGCCTAATTGCCCTC+CGG 0.574132 5_3:+52179263 None:intergenic
AGTAATCGCAAGACAAGAGA+TGG 0.579715 5_3:-52176367 Msa0817220:CDS
GCCCTTGAGATCAATATGAA+GGG 0.580105 5_3:+52176703 None:intergenic
TTGAGATCAATATGAAGGGA+AGG 0.580444 5_3:+52176707 None:intergenic
TTACATGAAATGTTGCATGT+TGG 0.580927 5_3:-52176427 Msa0817220:CDS
AACTCATTGATAACCTGGAG+GGG 0.583061 5_3:-52174983 Msa0817220:CDS
CAATGAGTTTGCAAGAGCAA+TGG 0.586120 5_3:-52174739 Msa0817220:exon
CGCTATATTCTCCATATGAG+TGG 0.586227 5_3:+52178221 None:intergenic
CAACTAAGAGATGACTTCAC+AGG 0.589863 5_3:-52177081 Msa0817220:intron
AACGAAAGAGAGACGGCGAG+AGG 0.594423 5_3:-52180007 Msa0817220:five_prime_UTR
AGAGACGGGAACGAGAGAGA+AGG 0.594857 5_3:-52180119 None:intergenic
TCCCTTCATATTGATCTCAA+GGG 0.595442 5_3:-52176704 Msa0817220:CDS
GCTGGAGCAGAAAAGGAAAA+CGG 0.595838 5_3:-52180064 Msa0817220:five_prime_UTR
ACTCATTGATAACCTGGAGG+GGG 0.601551 5_3:-52174982 Msa0817220:CDS
AAACTCATTGATAACCTGGA+GGG 0.604910 5_3:-52174984 Msa0817220:CDS
AACACGTGTAACAGGTACAA+AGG 0.609571 5_3:-52174879 Msa0817220:intron
CCGGAGGGCAATTAGGCTTG+AGG 0.610283 5_3:-52179263 Msa0817220:CDS
AATATGATTGTTAGCAAACG+GGG 0.610424 5_3:-52174481 Msa0817220:three_prime_UTR
GAGGGCAATTAGGCTTGAGG+TGG 0.611737 5_3:-52179260 Msa0817220:CDS
CAAGGACAACAATAAGGACA+TGG 0.614893 5_3:-52174715 Msa0817220:three_prime_UTR
CATGCTTCCTGCAGGGACAG+TGG 0.620249 5_3:-52179191 Msa0817220:CDS
TGAGATCAATATGAAGGGAA+GGG 0.626331 5_3:+52176708 None:intergenic
GTTTGCAAGAGCAATGGACA+AGG 0.626826 5_3:-52174733 Msa0817220:three_prime_UTR
GCTCCTAAAGATGAAAATTG+GGG 0.633816 5_3:-52179238 Msa0817220:CDS
CGCAAGACAAGAGATGGCCA+AGG 0.635388 5_3:-52176361 Msa0817220:CDS
GGTTATGTGAAATGATGAGC+CGG 0.636708 5_3:-52179282 Msa0817220:CDS
GTGAGGCCATGCTTCCTGCA+GGG 0.637114 5_3:-52179198 Msa0817220:CDS
TTTCAACATATCAGAGCGTG+AGG 0.644870 5_3:-52179215 Msa0817220:CDS
AGGTGAAAACGAAAGAGAGA+CGG 0.651922 5_3:-52180014 Msa0817220:five_prime_UTR
TATGTGAAATGATGAGCCGG+AGG 0.656757 5_3:-52179279 Msa0817220:CDS
CACTCATATGGAGAATATAG+CGG 0.659666 5_3:-52178220 Msa0817220:CDS
CCTTCAATGCATAGAAACAG+CGG 0.663560 5_3:+52174394 None:intergenic
ACGAGAGAGAAGGAACTCAA+CGG 0.674648 5_3:-52180109 Msa0817220:five_prime_UTR
ATGTGAAATGATGAGCCGGA+GGG 0.678863 5_3:-52179278 Msa0817220:CDS
AATGAAATGAATGAAGACCA+AGG 0.691600 5_3:-52179955 Msa0817220:intron
CATTTGTAAAGTCTCCGGCA+GGG 0.692662 5_3:+52174845 None:intergenic
AAAACTCATTGATAACCTGG+AGG 0.707690 5_3:-52174985 Msa0817220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTATTTTTAATTTTT+TGG - chr5_3:52178199-52178218 Msa0817220:CDS 0.0%
!!! AATTTTATTTTTAATTTTTT+GGG - chr5_3:52178200-52178219 Msa0817220:CDS 0.0%
!!! ATGTTTGTTTATTTTTAAAA+GGG - chr5_3:52174803-52174822 Msa0817220:CDS 10.0%
!!! AAATAATGCTTTTATTTTTT+AGG - chr5_3:52177841-52177860 Msa0817220:intron 10.0%
!! CTTAAATTTATAATCTAAAA+GGG + chr5_3:52179154-52179173 None:intergenic 10.0%
!! ATCATATTAAAAAAATTACA+AGG + chr5_3:52179881-52179900 None:intergenic 10.0%
!!! GATGTTTGTTTATTTTTAAA+AGG - chr5_3:52174802-52174821 Msa0817220:CDS 15.0%
!!! TTTCAATTGCAAAATATTTT+TGG - chr5_3:52175304-52175323 Msa0817220:intron 15.0%
!!! CTATTCTTTTTAAAATCAAT+TGG - chr5_3:52175900-52175919 Msa0817220:intron 15.0%
!! CTATTTACATGTTTATATAA+TGG - chr5_3:52176123-52176142 Msa0817220:intron 15.0%
!! TATAGGAAAAATAAAATAGA+GGG + chr5_3:52176403-52176422 None:intergenic 15.0%
!! ATATAGGAAAAATAAAATAG+AGG + chr5_3:52176404-52176423 None:intergenic 15.0%
!! ATCTATTCTTAAATCTTAAA+AGG + chr5_3:52176577-52176596 None:intergenic 15.0%
!!! AAAAAGTATTATTAGATTAG+AGG - chr5_3:52176693-52176712 Msa0817220:intron 15.0%
!! TTTATGTTTAACACTAATAA+TGG + chr5_3:52176780-52176799 None:intergenic 15.0%
!! ATAATAACAAAGAAAATCTT+GGG + chr5_3:52178473-52178492 None:intergenic 15.0%
!! AATAATAACAAAGAAAATCT+TGG + chr5_3:52178474-52178493 None:intergenic 15.0%
!! GCTTAAATTTATAATCTAAA+AGG + chr5_3:52179155-52179174 None:intergenic 15.0%
!!! TTTTGATTAAACAAAAGATG+AGG + chr5_3:52174499-52174518 None:intergenic 20.0%
!! GTTTAATCAAAAATCATGAT+CGG - chr5_3:52174507-52174526 Msa0817220:three_prime_UTR 20.0%
!!! TTTGTTTTTCCAGAATTTTT+TGG - chr5_3:52174590-52174609 Msa0817220:three_prime_UTR 20.0%
!!! GTTTTTTCGTTTTTAAGTTA+CGG - chr5_3:52174764-52174783 Msa0817220:CDS 20.0%
!!! ATCATTACTTTTTGTGTTTA+TGG - chr5_3:52175349-52175368 Msa0817220:intron 20.0%
!! GTGAAGAAAAAAAAATTGTT+AGG - chr5_3:52175406-52175425 Msa0817220:intron 20.0%
!! AATCCTTAAGATTCATAATA+TGG - chr5_3:52175565-52175584 Msa0817220:intron 20.0%
!! AAAACAATTAAACTTAAGCT+CGG + chr5_3:52175844-52175863 None:intergenic 20.0%
!!! AGCTTAAGTTTAATTGTTTT+TGG - chr5_3:52175844-52175863 Msa0817220:intron 20.0%
!! CTGAAAGAAGATTAATAATA+TGG + chr5_3:52176167-52176186 None:intergenic 20.0%
!! CATATTATTAATCTTCTTTC+AGG - chr5_3:52176165-52176184 Msa0817220:intron 20.0%
!! TATTAGTGTTAAACATAAAC+CGG - chr5_3:52176781-52176800 Msa0817220:intron 20.0%
!!! TATAAGTCTGAAATTTATTG+TGG - chr5_3:52177131-52177150 Msa0817220:CDS 20.0%
!! AAACTAACTAAACAAAAATC+AGG + chr5_3:52177191-52177210 None:intergenic 20.0%
!! CAAATTAAATCATACAATCA+TGG + chr5_3:52178162-52178181 None:intergenic 20.0%
!! CATGATTGTATGATTTAATT+TGG - chr5_3:52178160-52178179 Msa0817220:CDS 20.0%
!! ACAATTTAAATATGTCCATA+TGG - chr5_3:52178227-52178246 Msa0817220:CDS 20.0%
!! AATAAGATGATAGCAATTTA+TGG - chr5_3:52178375-52178394 Msa0817220:intron 20.0%
!! ATTTATATGAGCATAAATAG+TGG + chr5_3:52178437-52178456 None:intergenic 20.0%
!!! CATGTACTTTTTAGTATATT+TGG - chr5_3:52178843-52178862 Msa0817220:intron 20.0%
!!! TACTTTTTAGTATATTTGGT+GGG - chr5_3:52178847-52178866 Msa0817220:intron 20.0%
!!! TTTTCTTAAGAAATTAACCT+GGG - chr5_3:52179054-52179073 Msa0817220:intron 20.0%
!!! GATAATATATGCATGTATTT+TGG + chr5_3:52179364-52179383 None:intergenic 20.0%
!!! ATTCACTTTTAATAAAGAGA+GGG + chr5_3:52179807-52179826 None:intergenic 20.0%
! CTGAAGTGAAAAATGAAAAT+TGG - chr5_3:52174381-52174400 Msa0817220:three_prime_UTR 25.0%
! GAAAAATGAAAATTGGAAGA+AGG - chr5_3:52174388-52174407 Msa0817220:three_prime_UTR 25.0%
!! ATTCTGGAAAAACAAAAACT+TGG + chr5_3:52174586-52174605 None:intergenic 25.0%
!!! TTTTCCAGAATTTTTTGGAT+CGG - chr5_3:52174595-52174614 Msa0817220:three_prime_UTR 25.0%
! ATTGTCATGACATAAAATTG+GGG + chr5_3:52174702-52174721 None:intergenic 25.0%
! GATTGTCATGACATAAAATT+GGG + chr5_3:52174703-52174722 None:intergenic 25.0%
! TGATTGTCATGACATAAAAT+TGG + chr5_3:52174704-52174723 None:intergenic 25.0%
! AACGAAAAAACACAGTAAAA+AGG + chr5_3:52174755-52174774 None:intergenic 25.0%
!!! GTTACGGTTTTATTTTGAAT+TGG - chr5_3:52174780-52174799 Msa0817220:CDS 25.0%
! AAAGTTTAAAGATGACGAAA+TGG + chr5_3:52175022-52175041 None:intergenic 25.0%
! TTCTTCACATCAGTTAAAAT+GGG + chr5_3:52175394-52175413 None:intergenic 25.0%
! TTTCTTCACATCAGTTAAAA+TGG + chr5_3:52175395-52175414 None:intergenic 25.0%
! AAGAATGTTCAAATAAGATG+AGG - chr5_3:52175463-52175482 Msa0817220:intron 25.0%
! AGAATGTTCAAATAAGATGA+GGG - chr5_3:52175464-52175483 Msa0817220:intron 25.0%
! AAGCCATATTATGAATCTTA+AGG + chr5_3:52175571-52175590 None:intergenic 25.0%
! TTCTTCCAAAAAATCTGAAT+TGG - chr5_3:52175645-52175664 Msa0817220:intron 25.0%
! GTCATCTATAAGAAAACAAT+AGG + chr5_3:52175694-52175713 None:intergenic 25.0%
!! ATTGTTTTCTTATAGATGAC+AGG - chr5_3:52175694-52175713 Msa0817220:intron 25.0%
! ATCTCAATTGTTTCATACTT+TGG - chr5_3:52175991-52176010 Msa0817220:intron 25.0%
!!! TTCTTACTTTTGTGTTATCT+TGG - chr5_3:52176017-52176036 Msa0817220:intron 25.0%
! ACTAACCAAAAAACAGAAAA+GGG - chr5_3:52176082-52176101 Msa0817220:intron 25.0%
!!! TTTTTAATTCGACATGAAAG+TGG + chr5_3:52176262-52176281 None:intergenic 25.0%
! CGAATTAAAAAACAACCATT+TGG - chr5_3:52176271-52176290 Msa0817220:intron 25.0%
! TATGAGTAAAAGATGAAAAC+AGG - chr5_3:52176526-52176545 Msa0817220:intron 25.0%
! TCTTAAATCTTAAAAGGATG+AGG + chr5_3:52176571-52176590 None:intergenic 25.0%
! TTATTAGATTAGAGGTAGAA+TGG - chr5_3:52176701-52176720 Msa0817220:CDS 25.0%
! ATTGACATTAGGAAAAGAAA+AGG - chr5_3:52176838-52176857 Msa0817220:intron 25.0%
!! TAAAGGACAATCTTTTCTTT+GGG - chr5_3:52177022-52177041 Msa0817220:intron 25.0%
! TTGAATTGTTAATTGATGCT+TGG - chr5_3:52177209-52177228 Msa0817220:intron 25.0%
!!! CTTGGTATTTTTTATATACG+TGG - chr5_3:52177227-52177246 Msa0817220:intron 25.0%
!! AGCTAGATTTTAAATCATGT+AGG + chr5_3:52177758-52177777 None:intergenic 25.0%
! GAATTATGTGTTCTATTTGT+AGG - chr5_3:52177951-52177970 Msa0817220:intron 25.0%
!!! AAACTATCTTTTAGAGCTTT+TGG - chr5_3:52178351-52178370 Msa0817220:intron 25.0%
!! TATCTTTTAGATCTGTAAGA+AGG + chr5_3:52178638-52178657 None:intergenic 25.0%
! TCTAAAAGATATACCAAAGA+AGG - chr5_3:52178647-52178666 Msa0817220:intron 25.0%
!!! GTACTTTTTAGTATATTTGG+TGG - chr5_3:52178846-52178865 Msa0817220:intron 25.0%
!! GTTTTCTTAAGAAATTAACC+TGG - chr5_3:52179053-52179072 Msa0817220:intron 25.0%
!!! TGATATTTTATTTAGCGTGA+AGG - chr5_3:52179085-52179104 Msa0817220:intron 25.0%
!!! GATATTTTATTTAGCGTGAA+GGG - chr5_3:52179086-52179105 Msa0817220:intron 25.0%
!!! ATATTTTATTTAGCGTGAAG+GGG - chr5_3:52179087-52179106 Msa0817220:intron 25.0%
! AATACTAAATGTTAGCATAG+TGG + chr5_3:52179251-52179270 None:intergenic 25.0%
! TCTATTGAAGTTACATTTCT+TGG - chr5_3:52179280-52179299 Msa0817220:CDS 25.0%
! ATTCATTGATCTACATGTAT+AGG + chr5_3:52179320-52179339 None:intergenic 25.0%
! AGTAATTAAAGAGTTGTTTG+TGG + chr5_3:52179485-52179504 None:intergenic 25.0%
!!! CATTCACTTTTAATAAAGAG+AGG + chr5_3:52179808-52179827 None:intergenic 25.0%
! CAAATATGATTGTTAGCAAA+CGG - chr5_3:52179939-52179958 Msa0817220:intron 25.0%
! AAATATGATTGTTAGCAAAC+GGG - chr5_3:52179940-52179959 Msa0817220:intron 25.0%
AATGAAATGAATGAAGACCA+AGG - chr5_3:52174467-52174486 Msa0817220:three_prime_UTR 30.0%
AAAAGATGAGGAAATTACCT+TGG + chr5_3:52174487-52174506 None:intergenic 30.0%
AACATTACCAACCATTAAGT+TGG - chr5_3:52175069-52175088 Msa0817220:CDS 30.0%
AACATTACCAACTTAATGGT+TGG + chr5_3:52175079-52175098 None:intergenic 30.0%
! AAACCCCAATTTTCATCTTT+AGG + chr5_3:52175190-52175209 None:intergenic 30.0%
AGTGGATAACAATGTAGAAA+GGG - chr5_3:52175249-52175268 Msa0817220:intron 30.0%
TCATTTAAGTGTTTGTGAGA+TGG - chr5_3:52175763-52175782 Msa0817220:intron 30.0%
! GACAAAGGATATTTTTCACA+AGG - chr5_3:52175785-52175804 Msa0817220:CDS 30.0%
!! ATAGTTTTCACTTTTCAGTC+AGG - chr5_3:52176055-52176074 Msa0817220:intron 30.0%
CACTAACCAAAAAACAGAAA+AGG - chr5_3:52176081-52176100 Msa0817220:intron 30.0%
! AAGTGGAGAATTTTCACATT+GGG + chr5_3:52176245-52176264 None:intergenic 30.0%
! AAAGTGGAGAATTTTCACAT+TGG + chr5_3:52176246-52176265 None:intergenic 30.0%
! ATGATAGGAAAGGATTTGTT+AGG - chr5_3:52176335-52176354 Msa0817220:CDS 30.0%
!!! AGTTTGAGTTTTGAGTTTGA+GGG + chr5_3:52176443-52176462 None:intergenic 30.0%
!!! TAGTTTGAGTTTTGAGTTTG+AGG + chr5_3:52176444-52176463 None:intergenic 30.0%
AACTCAAAACTCAAACTACT+TGG - chr5_3:52176446-52176465 Msa0817220:CDS 30.0%
CAAACTTGGACATAGAATAA+TGG - chr5_3:52176724-52176743 Msa0817220:CDS 30.0%
TAATGGCGAATCAATAATTC+TGG - chr5_3:52176741-52176760 Msa0817220:CDS 30.0%
ACACTAATAATGGTACAACA+GGG + chr5_3:52176770-52176789 None:intergenic 30.0%
AACACTAATAATGGTACAAC+AGG + chr5_3:52176771-52176790 None:intergenic 30.0%
TGATATGACTCATTGACATT+AGG - chr5_3:52176827-52176846 Msa0817220:intron 30.0%
ACATTAGGAAAAGAAAAGGA+TGG - chr5_3:52176842-52176861 Msa0817220:intron 30.0%
!! TCAAGCCAAAGCTTTAAAAA+TGG - chr5_3:52176997-52177016 Msa0817220:intron 30.0%
!!! TTAGGCCATTTTTAAAGCTT+TGG + chr5_3:52177005-52177024 None:intergenic 30.0%
!! AAGCTTTAAAAATGGCCTAA+AGG - chr5_3:52177005-52177024 Msa0817220:intron 30.0%
CAAAGAAAAGATTGTCCTTT+AGG + chr5_3:52177023-52177042 None:intergenic 30.0%
! CTAAAGGACAATCTTTTCTT+TGG - chr5_3:52177021-52177040 Msa0817220:intron 30.0%
! AAGTCTGAAATTTATTGTGG+TGG - chr5_3:52177134-52177153 Msa0817220:CDS 30.0%
AGAAGCAAACATGTTATTCT+TGG - chr5_3:52177265-52177284 Msa0817220:intron 30.0%
AATGCTCTTGTTCTATATGA+TGG + chr5_3:52177515-52177534 None:intergenic 30.0%
! GATCATAAAACATGTTTTGC+AGG - chr5_3:52177643-52177662 Msa0817220:intron 30.0%
!!! CTTTTATTTTTTAGGCAGCT+GGG - chr5_3:52177849-52177868 Msa0817220:intron 30.0%
CTATTGTGTCAGAAAATTGA+GGG - chr5_3:52177898-52177917 Msa0817220:intron 30.0%
TTACATGAAATGTTGCATGT+TGG - chr5_3:52177995-52178014 Msa0817220:intron 30.0%
TCAAATTGCATTCTTCTGTT+TGG - chr5_3:52178090-52178109 Msa0817220:intron 30.0%
CATGGATAAGAAGATAACAA+TGG + chr5_3:52178144-52178163 None:intergenic 30.0%
! TGAACTCTTTAAAAGAGAAC+AGG - chr5_3:52178608-52178627 Msa0817220:intron 30.0%
TTATGATCACAAACCTTCTT+TGG + chr5_3:52178663-52178682 None:intergenic 30.0%
TCTATGATGAGATACATTTG+TGG - chr5_3:52178771-52178790 Msa0817220:intron 30.0%
CTATGATGAGATACATTTGT+GGG - chr5_3:52178772-52178791 Msa0817220:intron 30.0%
TATGATGAGATACATTTGTG+GGG - chr5_3:52178773-52178792 Msa0817220:intron 30.0%
CAATTATCTTTCAACTTCTG+TGG + chr5_3:52178822-52178841 None:intergenic 30.0%
CACAGAAGTTGAAAGATAAT+TGG - chr5_3:52178820-52178839 Msa0817220:intron 30.0%
GGTGGGAAAACATTAATAAA+AGG - chr5_3:52178864-52178883 Msa0817220:intron 30.0%
CAGCAAAAATTTGCATAACA+TGG + chr5_3:52178915-52178934 None:intergenic 30.0%
! TATTGATTACATAGCTGTTG+TGG - chr5_3:52178938-52178957 Msa0817220:intron 30.0%
! TAAGCTGAAATTCTAGAGAT+TGG + chr5_3:52178966-52178985 None:intergenic 30.0%
AATTTCAGTTCAAACTCTGA+TGG + chr5_3:52179198-52179217 None:intergenic 30.0%
! ACAAAAACTCATTGATAACC+TGG - chr5_3:52179434-52179453 Msa0817220:intron 30.0%
AAAATTGAAACACGTGTAAC+AGG - chr5_3:52179535-52179554 Msa0817220:intron 30.0%
AAATGTTGACCATTTCAAAG+AGG - chr5_3:52179595-52179614 Msa0817220:intron 30.0%
TCGACAACTTGTATCAATTT+CGG + chr5_3:52179662-52179681 None:intergenic 30.0%
GATTAGGAATGTCCTTAAAA+TGG + chr5_3:52179735-52179754 None:intergenic 30.0%
! TTGAATGAGAGGATCTATTT+TGG - chr5_3:52179757-52179776 Msa0817220:intron 30.0%
! TGAATGAGAGGATCTATTTT+GGG - chr5_3:52179758-52179777 Msa0817220:intron 30.0%
!! ATATGATGCCTTTTATTTGC+AGG - chr5_3:52179894-52179913 Msa0817220:intron 30.0%
AATATGATTGTTAGCAAACG+GGG - chr5_3:52179941-52179960 Msa0817220:intron 30.0%
! TTCTATACTCTTTTGCTTAG+TGG - chr5_3:52180062-52180081 Msa0817220:five_prime_UTR 30.0%
TTCTGATTCAAAATCCAGAG+CGG + chr5_3:52174662-52174681 None:intergenic 35.0%
AGATCGATTGAAGCAATGAA+AGG - chr5_3:52174871-52174890 Msa0817220:intron 35.0%
! ATTCATCATGCTTTGTTGAG+TGG - chr5_3:52174944-52174963 Msa0817220:intron 35.0%
AGACAATGGATCACATTGTT+GGG + chr5_3:52174973-52174992 None:intergenic 35.0%
GTTTAAAGATGACGAAATGG+AGG + chr5_3:52175019-52175038 None:intergenic 35.0%
GGAGAACATTACCAACTTAA+TGG + chr5_3:52175083-52175102 None:intergenic 35.0%
TGGCTCCTAAAGATGAAAAT+TGG - chr5_3:52175182-52175201 Msa0817220:intron 35.0%
GGCTCCTAAAGATGAAAATT+GGG - chr5_3:52175183-52175202 Msa0817220:intron 35.0%
GCTCCTAAAGATGAAAATTG+GGG - chr5_3:52175184-52175203 Msa0817220:intron 35.0%
CAGTGGATAACAATGTAGAA+AGG - chr5_3:52175248-52175267 Msa0817220:intron 35.0%
TGTAGAAAGGGTCTATAAAG+AGG - chr5_3:52175261-52175280 Msa0817220:intron 35.0%
! GGGTCTATAAAGAGGTATAA+TGG - chr5_3:52175269-52175288 Msa0817220:intron 35.0%
!!! CCTATCCTTTTCTAGAGTTT+GGG + chr5_3:52175517-52175536 None:intergenic 35.0%
!!! ACCTATCCTTTTCTAGAGTT+TGG + chr5_3:52175518-52175537 None:intergenic 35.0%
AGGATAGGTCAAATAAGATG+AGG - chr5_3:52175529-52175548 Msa0817220:intron 35.0%
!!! GAGCTCCAATTCAGATTTTT+TGG + chr5_3:52175653-52175672 None:intergenic 35.0%
AAAGCACACTTCTTCAAAGA+TGG + chr5_3:52175928-52175947 None:intergenic 35.0%
ACCAAAAAACAGAAAAGGGA+AGG - chr5_3:52176086-52176105 Msa0817220:intron 35.0%
CCAAAAAACAGAAAAGGGAA+GGG - chr5_3:52176087-52176106 Msa0817220:intron 35.0%
CAAAAAACAGAAAAGGGAAG+GGG - chr5_3:52176088-52176107 Msa0817220:intron 35.0%
TACCATATTTGCCACTCATA+TGG - chr5_3:52176190-52176209 Msa0817220:intron 35.0%
CACTCATATGGAGAATATAG+CGG - chr5_3:52176202-52176221 Msa0817220:intron 35.0%
! AAACAACCATTTGGAGTTCA+AGG - chr5_3:52176280-52176299 Msa0817220:intron 35.0%
ATACTACCTTGAACTCCAAA+TGG + chr5_3:52176289-52176308 None:intergenic 35.0%
!! TTCATTTTGACCTGCATGAT+AGG - chr5_3:52176320-52176339 Msa0817220:CDS 35.0%
GGGTATCATGCATGATATAT+AGG + chr5_3:52176420-52176439 None:intergenic 35.0%
!!! TTTGAGTTTTGAGTTTGAGG+GGG + chr5_3:52176441-52176460 None:intergenic 35.0%
!!! GTTTGAGTTTTGAGTTTGAG+GGG + chr5_3:52176442-52176461 None:intergenic 35.0%
ATCTTAAAAGGATGAGGACA+TGG + chr5_3:52176565-52176584 None:intergenic 35.0%
CGACTTGATTGGAATATTTC+AGG + chr5_3:52176932-52176951 None:intergenic 35.0%
! AATCGTATTCTCGACTTGAT+TGG + chr5_3:52176943-52176962 None:intergenic 35.0%
ATCAAGTCGAGAATACGATT+CGG - chr5_3:52176943-52176962 Msa0817220:CDS 35.0%
TCCTTAAGATTCATCAAAGC+TGG + chr5_3:52176969-52176988 None:intergenic 35.0%
TTATTCTTGGAATCTCCTGT+TGG - chr5_3:52177278-52177297 Msa0817220:intron 35.0%
TAAGAAAATCCAACTCCAAC+AGG + chr5_3:52177296-52177315 None:intergenic 35.0%
TCTTAGTTGTGCATATTCAC+TGG + chr5_3:52177330-52177349 None:intergenic 35.0%
! CTTACACTTTTCTGCACAAT+TGG - chr5_3:52177477-52177496 Msa0817220:intron 35.0%
!!! ACAAGAGCATTTTACATAGC+AGG - chr5_3:52177524-52177543 Msa0817220:intron 35.0%
TGAGATCAATATGAAGGGAA+GGG + chr5_3:52177717-52177736 None:intergenic 35.0%
TTGAGATCAATATGAAGGGA+AGG + chr5_3:52177718-52177737 None:intergenic 35.0%
TTCCCTTCATATTGATCTCA+AGG - chr5_3:52177717-52177736 Msa0817220:intron 35.0%
TCCCTTCATATTGATCTCAA+GGG - chr5_3:52177718-52177737 Msa0817220:intron 35.0%
!!! TATTGATCTCAAGGGCATTT+TGG - chr5_3:52177726-52177745 Msa0817220:intron 35.0%
!!! GCTTTTATTTTTTAGGCAGC+TGG - chr5_3:52177848-52177867 Msa0817220:intron 35.0%
GCTATTGTGTCAGAAAATTG+AGG - chr5_3:52177897-52177916 Msa0817220:intron 35.0%
AGCAGTTTCAAAGTTGTGTT+TGG - chr5_3:52178018-52178037 Msa0817220:intron 35.0%
ATGCAATTTGAGTATCTCCT+TGG + chr5_3:52178081-52178100 None:intergenic 35.0%
TGATAAGCAGTTATGCCATA+TGG + chr5_3:52178245-52178264 None:intergenic 35.0%
AAGAAAATCTTGGGTCTGTT+CGG + chr5_3:52178464-52178483 None:intergenic 35.0%
CTTGTGACATTGCCTTATAT+TGG - chr5_3:52178537-52178556 Msa0817220:intron 35.0%
TTGAAGGATCTGCCAATATA+AGG + chr5_3:52178552-52178571 None:intergenic 35.0%
! AGTACACCAATGTTGTTTAC+AGG + chr5_3:52178582-52178601 None:intergenic 35.0%
GTAAACAACATTGGTGTACT+TGG - chr5_3:52178582-52178601 Msa0817220:intron 35.0%
GCATAACATGGTACAAAATG+CGG + chr5_3:52178903-52178922 None:intergenic 35.0%
TCTTAAGAAATTAACCTGGG+AGG - chr5_3:52179057-52179076 Msa0817220:intron 35.0%
CTTAAGAAATTAACCTGGGA+GGG - chr5_3:52179058-52179077 Msa0817220:intron 35.0%
TTAAGAAATTAACCTGGGAG+GGG - chr5_3:52179059-52179078 Msa0817220:intron 35.0%
! AAAACTCATTGATAACCTGG+AGG - chr5_3:52179437-52179456 Msa0817220:intron 35.0%
! AAACTCATTGATAACCTGGA+GGG - chr5_3:52179438-52179457 Msa0817220:intron 35.0%
GTCAACATTTGTAAAGTCTC+CGG + chr5_3:52179585-52179604 None:intergenic 35.0%
AATGGACAAGGACAACAATA+AGG - chr5_3:52179701-52179720 Msa0817220:intron 35.0%
! AAGGACATGGATCCATTTTA+AGG - chr5_3:52179720-52179739 Msa0817220:intron 35.0%
TTCCTAATCTCTTGAATGAG+AGG - chr5_3:52179746-52179765 Msa0817220:intron 35.0%
ATCCTCTCATTCAAGAGATT+AGG + chr5_3:52179751-52179770 None:intergenic 35.0%
TCAACGGCAAAAAAGAGGAA+AGG - chr5_3:52174329-52174348 Msa0817220:three_prime_UTR 40.0%
AGGTGAAAACGAAAGAGAGA+CGG - chr5_3:52174408-52174427 Msa0817220:three_prime_UTR 40.0%
TGTTGCTATGCATGTTCGAT+TGG - chr5_3:52174556-52174575 Msa0817220:three_prime_UTR 40.0%
TGCTCCGATCCAAAAAATTC+TGG + chr5_3:52174602-52174621 None:intergenic 40.0%
!! GCTCTGGATTTTGAATCAGA+AGG - chr5_3:52174661-52174680 Msa0817220:three_prime_UTR 40.0%
CAGACAATGGATCACATTGT+TGG + chr5_3:52174974-52174993 None:intergenic 40.0%
ATGGAGGTAATTCAACTGGA+GGG + chr5_3:52175003-52175022 None:intergenic 40.0%
AATGGAGGTAATTCAACTGG+AGG + chr5_3:52175004-52175023 None:intergenic 40.0%
CGAAATGGAGGTAATTCAAC+TGG + chr5_3:52175007-52175026 None:intergenic 40.0%
TGCAGGTCTCTGTAGAATAT+GGG - chr5_3:52175119-52175138 Msa0817220:intron 40.0%
GGTTATGTGAAATGATGAGC+CGG - chr5_3:52175140-52175159 Msa0817220:intron 40.0%
TTTCAACATATCAGAGCGTG+AGG - chr5_3:52175207-52175226 Msa0817220:intron 40.0%
! ATGAGCCCAAACTCTAGAAA+AGG - chr5_3:52175509-52175528 Msa0817220:intron 40.0%
! CCCAAACTCTAGAAAAGGAT+AGG - chr5_3:52175514-52175533 Msa0817220:intron 40.0%
AGTGTTTGTGAGATGGACAA+AGG - chr5_3:52175770-52175789 Msa0817220:intron 40.0%
! CTTTTGTGTTATCTTGGCCA+TGG - chr5_3:52176023-52176042 Msa0817220:intron 40.0%
AAAACTATCACCATGCTCCA+TGG + chr5_3:52176043-52176062 None:intergenic 40.0%
!! CCCTTCCCTTTTCTGTTTTT+TGG + chr5_3:52176090-52176109 None:intergenic 40.0%
CTCCATATGAGTGGCAAATA+TGG + chr5_3:52176195-52176214 None:intergenic 40.0%
CGCTATATTCTCCATATGAG+TGG + chr5_3:52176204-52176223 None:intergenic 40.0%
TTTGACCTGCATGATAGGAA+AGG - chr5_3:52176325-52176344 Msa0817220:CDS 40.0%
CAAATCCTTTCCTATCATGC+AGG + chr5_3:52176333-52176352 None:intergenic 40.0%
GAAGAATGTATCAGCTGTGT+TGG - chr5_3:52176371-52176390 Msa0817220:CDS 40.0%
!!! TTGAGTTTTGAGTTTGAGGG+GGG + chr5_3:52176440-52176459 None:intergenic 40.0%
TGCTTACATGACATCACCTT+AGG - chr5_3:52176610-52176629 Msa0817220:intron 40.0%
AGAAAGTTCGCAGAAGATCA+AGG + chr5_3:52176662-52176681 None:intergenic 40.0%
TTGATCTTCTGCGAACTTTC+TGG - chr5_3:52176661-52176680 Msa0817220:intron 40.0%
! TGCGAACTTTCTGGTGAAAT+AGG - chr5_3:52176670-52176689 Msa0817220:intron 40.0%
TAGAGGTAGAATGGCAAACT+TGG - chr5_3:52176710-52176729 Msa0817220:CDS 40.0%
GTGCTTATCATGTTGCACAA+AGG - chr5_3:52176898-52176917 Msa0817220:CDS 40.0%
! GCCAGCTTTGATGAATCTTA+AGG - chr5_3:52176965-52176984 Msa0817220:intron 40.0%
TTATTGTGGTGGTCTTTGAG+AGG - chr5_3:52177145-52177164 Msa0817220:CDS 40.0%
CAACTAAGAGATGACTTCAC+AGG - chr5_3:52177341-52177360 Msa0817220:intron 40.0%
GCCCTTGAGATCAATATGAA+GGG + chr5_3:52177722-52177741 None:intergenic 40.0%
TGCCCTTGAGATCAATATGA+AGG + chr5_3:52177723-52177742 None:intergenic 40.0%
TCTCATGTTGCAAGTGATAG+AGG - chr5_3:52177923-52177942 Msa0817220:intron 40.0%
AGTAATCGCAAGACAAGAGA+TGG - chr5_3:52178055-52178074 Msa0817220:intron 40.0%
ACATGGTACAAAATGCGGTA+AGG + chr5_3:52178898-52178917 None:intergenic 40.0%
TAAGAAATTAACCTGGGAGG+GGG - chr5_3:52179060-52179079 Msa0817220:intron 40.0%
GCTTGGCATGTAATCTAAAG+AGG + chr5_3:52179223-52179242 None:intergenic 40.0%
TTAGCATAGTGGTAACTGCT+TGG + chr5_3:52179240-52179259 None:intergenic 40.0%
!!! TATGCATGTATTTTGGCAGC+TGG + chr5_3:52179357-52179376 None:intergenic 40.0%
AAGCAAATGCCTATCGTGAT+AGG - chr5_3:52179396-52179415 Msa0817220:intron 40.0%
AGCAAATGCCTATCGTGATA+GGG - chr5_3:52179397-52179416 Msa0817220:intron 40.0%
! TTAGCTTTCCCTATCACGAT+AGG + chr5_3:52179408-52179427 None:intergenic 40.0%
! AACTCATTGATAACCTGGAG+GGG - chr5_3:52179439-52179458 Msa0817220:intron 40.0%
AACACGTGTAACAGGTACAA+AGG - chr5_3:52179543-52179562 Msa0817220:intron 40.0%
ACACGTGTAACAGGTACAAA+GGG - chr5_3:52179544-52179563 Msa0817220:intron 40.0%
! CACTCAACACCTCTTTGAAA+TGG + chr5_3:52179607-52179626 None:intergenic 40.0%
CAATGAGTTTGCAAGAGCAA+TGG - chr5_3:52179683-52179702 Msa0817220:intron 40.0%
CAAGGACAACAATAAGGACA+TGG - chr5_3:52179707-52179726 Msa0817220:intron 40.0%
CCTTCAATGCATAGAAACAG+CGG + chr5_3:52180031-52180050 None:intergenic 40.0%
ACGAGAGAGAAGGAACTCAA+CGG - chr5_3:52174313-52174332 Msa0817220:three_prime_UTR 45.0%
GGAACTCAACGGCAAAAAAG+AGG - chr5_3:52174324-52174343 Msa0817220:three_prime_UTR 45.0%
ACGGCAAAAAAGAGGAAAGG+AGG - chr5_3:52174332-52174351 Msa0817220:three_prime_UTR 45.0%
GCTGGAGCAGAAAAGGAAAA+CGG - chr5_3:52174358-52174377 Msa0817220:three_prime_UTR 45.0%
!! AGGAGAGGAAAGTGTTGTGT+TGG - chr5_3:52174435-52174454 Msa0817220:three_prime_UTR 45.0%
TTGGATCGGAGCAACTTCAA+CGG - chr5_3:52174609-52174628 Msa0817220:three_prime_UTR 45.0%
TGATTCAAAATCCAGAGCGG+CGG + chr5_3:52174659-52174678 None:intergenic 45.0%
TGGAGGGATATCACAGACAA+TGG + chr5_3:52174987-52175006 None:intergenic 45.0%
CTGCAGGTCTCTGTAGAATA+TGG - chr5_3:52175118-52175137 Msa0817220:intron 45.0%
TATGTGAAATGATGAGCCGG+AGG - chr5_3:52175143-52175162 Msa0817220:intron 45.0%
ATGTGAAATGATGAGCCGGA+GGG - chr5_3:52175144-52175163 Msa0817220:intron 45.0%
AGAAAAGGGAAGGGGTGAAA+AGG - chr5_3:52176096-52176115 Msa0817220:intron 45.0%
AGGATGAGGACATGGAACAA+TGG + chr5_3:52176557-52176576 None:intergenic 45.0%
TTAGTGTCTGTGCTTAGTGC+CGG + chr5_3:52176803-52176822 None:intergenic 45.0%
TTGGAATCTCCTGTTGGAGT+TGG - chr5_3:52177284-52177303 Msa0817220:intron 45.0%
! GTTTACAGGCTGGTCATTGA+AGG + chr5_3:52178568-52178587 None:intergenic 45.0%
GACCAGCCTGTAAACAACAT+TGG - chr5_3:52178573-52178592 Msa0817220:intron 45.0%
! CACCAATGTTGTTTACAGGC+TGG + chr5_3:52178578-52178597 None:intergenic 45.0%
AAAATATCATTCCCCCTCCC+AGG + chr5_3:52179074-52179093 None:intergenic 45.0%
! ACTCATTGATAACCTGGAGG+GGG - chr5_3:52179440-52179459 Msa0817220:intron 45.0%
CATTTGTAAAGTCTCCGGCA+GGG + chr5_3:52179580-52179599 None:intergenic 45.0%
ACATTTGTAAAGTCTCCGGC+AGG + chr5_3:52179581-52179600 None:intergenic 45.0%
GTTTGCAAGAGCAATGGACA+AGG - chr5_3:52179689-52179708 Msa0817220:intron 45.0%
!! GCCTTTTATTTGCAGGTACG+TGG - chr5_3:52179901-52179920 Msa0817220:intron 45.0%
GCCACGTACCTGCAAATAAA+AGG + chr5_3:52179905-52179924 None:intergenic 45.0%
!! CCGCTGTTTCTATGCATTGA+AGG - chr5_3:52180028-52180047 Msa0817220:five_prime_UTR 45.0%
!! TATTTCATTTATTTATAATA+TGG - chr5_3:52175621-52175640 Msa0817220:intron 5.0%
AAAGAGGAAAGGAGGAACGC+TGG - chr5_3:52174340-52174359 Msa0817220:three_prime_UTR 50.0%
CTGCAGTTGTGGAAGAGAGT+TGG + chr5_3:52175104-52175123 None:intergenic 50.0%
CAACTCTCTTCCACAACTGC+AGG - chr5_3:52175102-52175121 Msa0817220:intron 50.0%
TCTACAGAGACCTGCAGTTG+TGG + chr5_3:52175115-52175134 None:intergenic 50.0%
TTGTTATCCACTGTCCCTGC+AGG + chr5_3:52175241-52175260 None:intergenic 50.0%
GTTATCTTGGCCATGGAGCA+TGG - chr5_3:52176030-52176049 Msa0817220:intron 50.0%
CCTCGCTATGCAGAATCCTA+AGG + chr5_3:52176629-52176648 None:intergenic 50.0%
CCTTAGGATTCTGCATAGCG+AGG - chr5_3:52176626-52176645 Msa0817220:intron 50.0%
ATAGCAGGAGAGAGCTATGC+AGG - chr5_3:52177539-52177558 Msa0817220:intron 50.0%
CCAATCCTGCTCACTCTTCA+GGG + chr5_3:52177878-52177897 None:intergenic 50.0%
GAGGAACGCTGGAGCAGAAA+AGG - chr5_3:52174351-52174370 Msa0817220:three_prime_UTR 55.0%
AACGAAAGAGAGACGGCGAG+AGG - chr5_3:52174415-52174434 Msa0817220:three_prime_UTR 55.0%
TGATGAGCCGGAGGGCAATT+AGG - chr5_3:52175152-52175171 Msa0817220:intron 55.0%
CCTCAAGCCTAATTGCCCTC+CGG + chr5_3:52175162-52175181 None:intergenic 55.0%
GAGGGCAATTAGGCTTGAGG+TGG - chr5_3:52175162-52175181 Msa0817220:intron 55.0%
GGAAACCCTGAAGAGTGAGC+AGG - chr5_3:52177870-52177889 Msa0817220:intron 55.0%
CCCTGAAGAGTGAGCAGGAT+TGG - chr5_3:52177875-52177894 Msa0817220:intron 55.0%
GCCAATCCTGCTCACTCTTC+AGG + chr5_3:52177879-52177898 None:intergenic 55.0%
CGCAAGACAAGAGATGGCCA+AGG - chr5_3:52178061-52178080 Msa0817220:intron 55.0%
TAAAGTCTCCGGCAGGGAGA+TGG + chr5_3:52179574-52179593 None:intergenic 55.0%
AAGAGAGACGGCGAGAGGAG+AGG - chr5_3:52174420-52174439 Msa0817220:three_prime_UTR 60.0%
GGAGCAACTTCAACGGCGAC+CGG - chr5_3:52174616-52174635 Msa0817220:three_prime_UTR 60.0%
GGAAACGATTGCCGGTGCAC+CGG + chr5_3:52174638-52174657 None:intergenic 60.0%
AGAGCGGCGGAAACGATTGC+CGG + chr5_3:52174646-52174665 None:intergenic 60.0%
CCGGAGGGCAATTAGGCTTG+AGG - chr5_3:52175159-52175178 Msa0817220:intron 60.0%
GTGAGGCCATGCTTCCTGCA+GGG - chr5_3:52175224-52175243 Msa0817220:intron 60.0%
CACTGTCCCTGCAGGAAGCA+TGG + chr5_3:52175233-52175252 None:intergenic 60.0%
CATGCTTCCTGCAGGGACAG+TGG - chr5_3:52175231-52175250 Msa0817220:intron 60.0%
CCTTCGTGAATTCCCCCTCC+AGG + chr5_3:52179455-52179474 None:intergenic 60.0%
CCTGGAGGGGGAATTCACGA+AGG - chr5_3:52179452-52179471 Msa0817220:intron 60.0%
AGGGAGTTCCATCTCCCTGC+CGG - chr5_3:52179563-52179582 Msa0817220:intron 60.0%
CAGGTACGTGGCAAGCGAGT+TGG - chr5_3:52179913-52179932 Msa0817220:intron 60.0%
TTCAACGGCGACCGGTGCAC+CGG - chr5_3:52174624-52174643 Msa0817220:three_prime_UTR 65.0%
GGCAATCGTTTCCGCCGCTC+TGG - chr5_3:52174645-52174664 Msa0817220:three_prime_UTR 65.0%
CGTGAGGCCATGCTTCCTGC+AGG - chr5_3:52175223-52175242 Msa0817220:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 52174308 52180136 52174308 ID=Msa0817220;Name=Msa0817220
chr5_3 mRNA 52174308 52180136 52174308 ID=Msa0817220-mRNA-1;Parent=Msa0817220;Name=Msa0817220-mRNA-1;_AED=0.29;_eAED=0.29;_QI=169|0.4|0.72|1|0.8|0.81|11|448|349
chr5_3 exon 52174308 52174887 52174308 ID=Msa0817220-mRNA-1:exon:15686;Parent=Msa0817220-mRNA-1
chr5_3 exon 52174971 52175114 52174971 ID=Msa0817220-mRNA-1:exon:15685;Parent=Msa0817220-mRNA-1
chr5_3 exon 52175776 52175821 52175776 ID=Msa0817220-mRNA-1:exon:15684;Parent=Msa0817220-mRNA-1
chr5_3 exon 52176319 52176471 52176319 ID=Msa0817220-mRNA-1:exon:15683;Parent=Msa0817220-mRNA-1
chr5_3 exon 52176561 52176577 52176561 ID=Msa0817220-mRNA-1:exon:15682;Parent=Msa0817220-mRNA-1
chr5_3 exon 52176697 52176779 52176697 ID=Msa0817220-mRNA-1:exon:15681;Parent=Msa0817220-mRNA-1
chr5_3 exon 52176884 52176976 52176884 ID=Msa0817220-mRNA-1:exon:15680;Parent=Msa0817220-mRNA-1
chr5_3 exon 52177082 52177177 52177082 ID=Msa0817220-mRNA-1:exon:15679;Parent=Msa0817220-mRNA-1
chr5_3 exon 52178143 52178257 52178143 ID=Msa0817220-mRNA-1:exon:15678;Parent=Msa0817220-mRNA-1
chr5_3 exon 52179162 52179320 52179162 ID=Msa0817220-mRNA-1:exon:15677;Parent=Msa0817220-mRNA-1
chr5_3 exon 52179956 52180136 52179956 ID=Msa0817220-mRNA-1:exon:15676;Parent=Msa0817220-mRNA-1
chr5_3 five_prime_UTR 52179968 52180136 52179968 ID=Msa0817220-mRNA-1:five_prime_utr;Parent=Msa0817220-mRNA-1
chr5_3 CDS 52179956 52179967 52179956 ID=Msa0817220-mRNA-1:cds;Parent=Msa0817220-mRNA-1
chr5_3 CDS 52179162 52179320 52179162 ID=Msa0817220-mRNA-1:cds;Parent=Msa0817220-mRNA-1
chr5_3 CDS 52178143 52178257 52178143 ID=Msa0817220-mRNA-1:cds;Parent=Msa0817220-mRNA-1
chr5_3 CDS 52177082 52177177 52177082 ID=Msa0817220-mRNA-1:cds;Parent=Msa0817220-mRNA-1
chr5_3 CDS 52176884 52176976 52176884 ID=Msa0817220-mRNA-1:cds;Parent=Msa0817220-mRNA-1
chr5_3 CDS 52176697 52176779 52176697 ID=Msa0817220-mRNA-1:cds;Parent=Msa0817220-mRNA-1
chr5_3 CDS 52176561 52176577 52176561 ID=Msa0817220-mRNA-1:cds;Parent=Msa0817220-mRNA-1
chr5_3 CDS 52176319 52176471 52176319 ID=Msa0817220-mRNA-1:cds;Parent=Msa0817220-mRNA-1
chr5_3 CDS 52175776 52175821 52175776 ID=Msa0817220-mRNA-1:cds;Parent=Msa0817220-mRNA-1
chr5_3 CDS 52174971 52175114 52174971 ID=Msa0817220-mRNA-1:cds;Parent=Msa0817220-mRNA-1
chr5_3 CDS 52174756 52174887 52174756 ID=Msa0817220-mRNA-1:cds;Parent=Msa0817220-mRNA-1
chr5_3 three_prime_UTR 52174308 52174755 52174308 ID=Msa0817220-mRNA-1:three_prime_utr;Parent=Msa0817220-mRNA-1
Gene Sequence

>Msa0817220

ATGAAGACCAAGGTCTCTGTAGAATATGGGTTATGTGAAATGATGAGCCGGAGGGCAATTAGGCTTGAGGTGGCTCCTAAAGATGAAAATTGGGGTTTCAACATATCAGAGCGTGAGGCCATGCTTCCTGCAGGGACAGTGGATAACAATGTAGAAAGGGTCTATAAAGAGGTATACCATATTTGCCACTCATATGGAGAATATAGCGGAATTAGCAACAATCTATCCCAATGTGAAAATTCTCCACTTTCATGTCGAATTAAAAAACAACCATTTGGAGTTCAAGAAGCAAACATGTTATTCTTGGAATCTCCTGTTGGAGTTGGATTTTCTTACTCAAATACAACCAGTGAATATGCACAACTAAGAGATGACTTCACAGCTAATGATGCTTACACTTTTCTGCACAATTGGTTTCTCAAGTATCCATCATATAGAACAAGAGCATTTTACATAGCAGGAGAGAGCTATGCAGGAAAGTACGTACTAGAGCTTGCTGAACTCATCATTGATAGAAACAATAACCCTTCCCTTCATATTGATCTCAAGGGCATTTTGCTGGGAAACCCTGAAGAGTATTCTGCAAATTCTAAAGTTTTACATGAAATGTTGCATGTTGGAGCAGTTTCAAAGTTGTGTTTGGTGCTTCAAGTTGATAGTAATCGCAAGACAAGAGATGGCCAAGGAGATACTCAAATTGCATTCTTCTGTTTGGAAGAATTCTACATAGAACAGGAAGACCTTCTTACAGATCTAAAAGATATACCAAAGAAGATCAATGAATTTGTAAAACGAGCTCCAGCTGCCAAAATACATGCATATATTATCAGTCATCTGAAAAAGCAAATGCCTATCGTGATAGGGAAAGCTAAAGTTCAACAAAAACTCATTGATAACCTGGAGGGGGAATTCACGAAGGTACAAAGGGAGTTCCATCTCCCTGCCGGAGACTTTACAAATGTTGACCATTTCAAAGAGGTGTTGAGTGTTTATAACATCGACAAGTTTGAGAAATTGAAACCGAAATTGATACAAGTTGTCGATCAATGA
Protein sequence

>Msa0817220

MKTKVSVEYGLCEMMSRRAIRLEVAPKDENWGFNISEREAMLPAGTVDNNVERVYKEVYHICHSYGEYSGISNNLSQCENSPLSCRIKKQPFGVQEANMLFLESPVGVGFSYSNTTSEYAQLRDDFTANDAYTFLHNWFLKYPSYRTRAFYIAGESYAGKYVLELAELIIDRNNNPSLHIDLKGILLGNPEEYSANSKVLHEMLHVGAVSKLCLVLQVDSNRKTRDGQGDTQIAFFCLEEFYIEQEDLLTDLKDIPKKINEFVKRAPAAKIHAYIISHLKKQMPIVIGKAKVQQKLIDNLEGEFTKVQREFHLPAGDFTNVDHFKEVLSVYNIDKFEKLKPKLIQVVDQ*