AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0566160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0566160 MtrunA17_Chr8g0380611 99.735 377 1 0 1 377 1 377 0.0 779
Msa0566160 MtrunA17_Chr2g0316551 34.572 269 145 8 75 330 60 310 1.53e-27 111
Msa0566160 MtrunA17_Chr2g0325131 30.841 321 168 11 22 324 2 286 2.92e-26 107
Msa0566160 MtrunA17_Chr4g0040891 52.885 104 44 1 228 326 188 291 5.47e-24 101
Msa0566160 MtrunA17_Chr2g0329551 27.372 274 163 6 80 328 58 320 6.54e-13 69.7
Msa0566160 MtrunA17_Chr6g0463571 29.530 298 147 14 75 337 11 280 1.58e-12 67.8
Msa0566160 MtrunA17_Chr4g0036071 37.705 122 70 2 206 321 331 452 2.93e-12 67.8
Msa0566160 MtrunA17_Chr5g0432701 36.567 134 61 4 233 343 158 290 3.40e-12 67.0
Msa0566160 MtrunA17_Chr3g0107491 41.935 93 49 1 234 321 177 269 2.45e-11 64.7
Msa0566160 MtrunA17_Chr3g0128781 34.127 126 63 2 233 338 107 232 7.61e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0566160 AT1G58110.1 52.139 374 164 7 1 369 1 364 9.46e-114 336
Msa0566160 AT1G58110.2 52.139 374 164 7 1 369 1 364 9.46e-114 336
Msa0566160 AT4G06598.2 47.601 271 131 5 1 266 1 265 1.13e-64 207
Msa0566160 AT4G06598.1 47.601 271 131 5 1 266 1 265 1.13e-64 207
Msa0566160 AT1G35490.2 35.783 313 141 10 58 359 37 300 6.24e-42 149
Msa0566160 AT1G35490.1 35.783 313 141 10 58 359 37 300 6.24e-42 149
Msa0566160 AT2G42380.2 31.889 323 161 11 22 326 2 283 2.64e-28 113
Msa0566160 AT3G58120.1 55.056 89 40 0 238 326 211 299 3.31e-23 99.4
Msa0566160 AT5G04840.1 31.475 305 155 13 22 321 5 260 1.45e-22 97.1
Msa0566160 AT2G42380.5 30.363 303 152 11 22 306 2 263 1.07e-21 94.0
Msa0566160 AT2G42380.1 29.721 323 157 12 22 326 2 272 3.50e-21 93.2
Msa0566160 AT5G04840.2 31.379 290 145 13 22 306 5 245 1.56e-20 90.1
Msa0566160 AT3G58120.2 55.072 69 31 0 238 306 211 279 6.80e-17 80.5
Msa0566160 AT1G06070.1 28.517 263 158 6 80 326 59 307 1.43e-14 75.1
Msa0566160 AT2G40620.1 41.489 94 50 1 233 321 147 240 3.30e-11 64.7
Msa0566160 AT2G31370.5 38.017 121 62 2 219 326 178 298 5.71e-11 63.9
Msa0566160 AT2G31370.2 28.897 263 151 7 86 326 50 298 7.15e-11 63.5
Msa0566160 AT2G31370.4 28.897 263 151 7 86 326 50 298 7.15e-11 63.5
Msa0566160 AT2G31370.1 28.897 263 151 7 86 326 50 298 7.15e-11 63.5
Msa0566160 AT2G31370.7 28.897 263 151 7 86 326 50 298 7.15e-11 63.5
Msa0566160 AT2G31370.3 28.897 263 151 7 86 326 50 298 7.15e-11 63.5
Msa0566160 AT2G31370.6 28.897 263 151 7 86 326 50 298 7.15e-11 63.5
Msa0566160 AT1G43700.1 41.935 93 49 1 234 321 194 286 8.01e-11 63.2
Msa0566160 AT4G38900.2 38.298 94 53 1 233 321 393 486 9.55e-11 63.5
Msa0566160 AT4G38900.3 38.298 94 53 1 233 321 393 486 9.55e-11 63.5

Find 139 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGGCTTATTAACCGGCTT+TGG 0.246012 4_1:+67181187 None:intergenic
CCAAGGCTTGAAAGCTTTAG+AGG 0.255775 4_1:-67178202 Msa0566160:three_prime_UTR
ATCTCCATTGTGTTTGAATT+TGG 0.257811 4_1:-67178404 Msa0566160:three_prime_UTR
ATATAAGAATTTATCTATTC+CGG 0.267904 4_1:-67180963 Msa0566160:CDS
TAAATAAATGGCAATAGTTT+AGG 0.290149 4_1:+67178570 None:intergenic
CCCCTTCTGACAGGTGTATC+TGG 0.293268 4_1:+67181076 None:intergenic
CAGGCGCAACATTAAATAAA+TGG 0.301018 4_1:+67178558 None:intergenic
GGTACTAGGAAATGGGCTTT+TGG 0.301960 4_1:+67181252 None:intergenic
AGAAATTAGAATTAAGATTT+CGG 0.302093 4_1:+67178525 None:intergenic
CTCTGCTGTTTGTGCGTCTA+TGG 0.316760 4_1:+67178847 None:intergenic
CCTTCTCGTGGCTTATTAAC+CGG 0.320223 4_1:+67181181 None:intergenic
AGGTATTCATCCTGCTTCTA+TGG 0.322222 4_1:-67181347 Msa0566160:exon
GAAGATTCGAGATCCCTAAA+AGG 0.327964 4_1:+67182534 None:intergenic
GCAGAATCTTATCCTGAGTA+TGG 0.345181 4_1:-67179938 Msa0566160:CDS
AAGAAATGTACAGGCTTTAC+AGG 0.348529 4_1:-67180117 Msa0566160:intron
TACATAATCACTGAAGATTT+CGG 0.349142 4_1:+67178448 None:intergenic
GTGGCTTATTAACCGGCTTT+GGG 0.351720 4_1:+67181188 None:intergenic
AATGTGCATTATTATTTGTA+TGG 0.354445 4_1:-67178346 Msa0566160:three_prime_UTR
TGATGCAATGTGTAACTAAA+TGG 0.354954 4_1:+67182379 None:intergenic
AAGCACAAGGACACAAATTC+GGG 0.361944 4_1:-67178792 Msa0566160:CDS
AGAGCATGTGCATGCCTTCC+AGG 0.362446 4_1:+67181283 None:intergenic
GAAGTACTGGAGAGAGAGAT+TGG 0.385340 4_1:-67178939 Msa0566160:CDS
GAAGCACAAGGACACAAATT+CGG 0.386149 4_1:-67178793 Msa0566160:CDS
CCTCTAAAGCTTTCAAGCCT+TGG 0.389205 4_1:+67178202 None:intergenic
TCTTTGTTGCGGTCAAAGTC+TGG 0.389808 4_1:+67180929 None:intergenic
GCTTGGGAGGTACTAGGAAA+TGG 0.394747 4_1:+67181244 None:intergenic
GGGATTGTGTAGAGATTTCC+AGG 0.398765 4_1:-67178664 Msa0566160:three_prime_UTR
AGATATCTGCTATTTGCATT+AGG 0.402101 4_1:+67178381 None:intergenic
CTGTCAGAAGGGGTGGTCAT+AGG 0.403970 4_1:-67181066 Msa0566160:CDS
TTCAGTGGAGCAGGAAGTAC+TGG 0.406294 4_1:-67178952 Msa0566160:intron
TGTCCTTGTGCTTCAGAGAA+AGG 0.416097 4_1:+67178802 None:intergenic
ATGCAGCAAAACAGAGGAAT+AGG 0.418189 4_1:-67180874 Msa0566160:CDS
ATTCTGAGTGCTCCGTTCGC+AGG 0.418833 4_1:+67178762 None:intergenic
GGCAATTTGCTCAACGGTCA+CGG 0.436276 4_1:-67180172 Msa0566160:CDS
CAGGTGTATCTGGCTCATTT+AGG 0.448183 4_1:+67181086 None:intergenic
GTCAGAAATTTGATGTATCC+TGG 0.451042 4_1:-67181301 Msa0566160:CDS
CGGCTATGTACTGAAGTTTA+CGG 0.451786 4_1:+67180147 None:intergenic
AATATGAGTGGTGTTCCATC+TGG 0.452901 4_1:-67180833 Msa0566160:CDS
AACAAGTTACAAGTATTAGT+TGG 0.457857 4_1:-67182664 Msa0566160:five_prime_UTR
AAGATTCGAGATCCCTAAAA+GGG 0.468317 4_1:+67182535 None:intergenic
AGACATAATCAGCATATGCT+TGG 0.479211 4_1:+67181227 None:intergenic
AATTCAAAATTCAGTGGAGC+AGG 0.479812 4_1:-67178961 Msa0566160:intron
GCATATGCTTGGGAGGTACT+AGG 0.481407 4_1:+67181238 None:intergenic
CAGAGAGAGAGGGCATATCC+TGG 0.482736 4_1:+67178646 None:intergenic
TTTGTTTCCTTTCGAAATGC+GGG 0.484540 4_1:-67178684 Msa0566160:three_prime_UTR
ATGCAAATAGCAGATATCTC+GGG 0.485690 4_1:-67178377 Msa0566160:three_prime_UTR
GCGAATTCAAAGAGCTCATC+GGG 0.486195 4_1:-67181325 Msa0566160:CDS
GTGCCTGCTGCAGATGCTGT+TGG 0.488082 4_1:+67178898 None:intergenic
TTTGAATTCGCCATAGAAGC+AGG 0.488523 4_1:+67181337 None:intergenic
ATTTCTGTCACTTGACCTGC+CGG 0.492212 4_1:-67178508 Msa0566160:three_prime_UTR
GACATAATCAGCATATGCTT+GGG 0.492590 4_1:+67181228 None:intergenic
CTTGGGAGGTACTAGGAAAT+GGG 0.499593 4_1:+67181245 None:intergenic
ACCGGCTTTGGGCCAACTGT+AGG 0.503588 4_1:+67181199 None:intergenic
GTCACTTGACCTGCCGGAGG+TGG 0.505415 4_1:-67178502 Msa0566160:three_prime_UTR
AGAATTTATCTATTCCGGCT+TGG 0.507292 4_1:-67180958 Msa0566160:CDS
CTGTACATTTCTTTCCAGCT+CGG 0.509414 4_1:+67180127 None:intergenic
CCGGCTTTGGGCCAACTGTA+GGG 0.510212 4_1:+67181200 None:intergenic
AATGCAAATAGCAGATATCT+CGG 0.511907 4_1:-67178378 Msa0566160:three_prime_UTR
TTCTGAGTGCTCCGTTCGCA+GGG 0.512290 4_1:+67178763 None:intergenic
CTCTTCTTGTTGAAGCTGTG+TGG 0.512359 4_1:+67182629 None:intergenic
AACCATCGAGTTACCACCTC+CGG 0.513802 4_1:+67178489 None:intergenic
GCAATTTGCTCAACGGTCAC+GGG 0.514889 4_1:-67180171 Msa0566160:CDS
GGCAGAAGGGTCTGAAGTAT+CGG 0.519383 4_1:-67179983 Msa0566160:CDS
GCAGTCAGTAGGATCCGATC+TGG 0.520904 4_1:+67180752 None:intergenic
TAATCACTGAAGATTTCGGC+AGG 0.523111 4_1:+67178452 None:intergenic
CGCTGAAATAGCAGCTACAC+GGG 0.523495 4_1:+67178623 None:intergenic
CGAGCTGGAAAGAAATGTAC+AGG 0.524315 4_1:-67180126 Msa0566160:CDS
ATGGCTGCTCCTCGATAAGA+TGG 0.524860 4_1:+67181125 None:intergenic
CAGCCTCAACAACAACCATC+TGG 0.528277 4_1:-67178873 Msa0566160:CDS
GCATTGATTTCTGCATACAT+AGG 0.528831 4_1:+67180893 None:intergenic
CATTTATTTAATGTTGCGCC+TGG 0.530403 4_1:-67178557 Msa0566160:three_prime_UTR
CCCTACAGTTGGCCCAAAGC+CGG 0.530439 4_1:-67181200 Msa0566160:CDS
CTGCCAGATGGTTGTTGTTG+AGG 0.530949 4_1:+67178870 None:intergenic
GCTTCAACAAGAAGAGAGAC+AGG 0.531578 4_1:-67182622 Msa0566160:five_prime_UTR
GTGCTTTATTCTCCATACTC+AGG 0.532806 4_1:+67179926 None:intergenic
GCAACATTGTCTTTGCCAGA+TGG 0.534238 4_1:+67180818 None:intergenic
GCCAGATACACCTGTCAGAA+GGG 0.539636 4_1:-67181077 Msa0566160:CDS
TACTCAGGATAAGATTCTGC+TGG 0.540325 4_1:+67179941 None:intergenic
AAGGAAACAAATTCTATCAC+AGG 0.544577 4_1:+67178696 None:intergenic
AGCTACACGGGCAGAGAGAG+AGG 0.545113 4_1:+67178635 None:intergenic
CTATGACCACCCCTTCTGAC+AGG 0.545707 4_1:+67181067 None:intergenic
TGATGTGAGTTTCCTTCTCG+TGG 0.547292 4_1:+67181169 None:intergenic
CAAAACAGAGGAATAGGTCA+TGG 0.548013 4_1:-67180868 Msa0566160:CDS
GGCGAATTCAAAGAGCTCAT+CGG 0.549095 4_1:-67181326 Msa0566160:CDS
AGATTTCGGCAATGACCACC+AGG 0.550245 4_1:+67178539 None:intergenic
AGCCAGATACACCTGTCAGA+AGG 0.551012 4_1:-67181078 Msa0566160:CDS
ATCACTGAAGATTTCGGCAG+GGG 0.552110 4_1:+67178454 None:intergenic
TATGACAATACATAGGCAGA+AGG 0.552452 4_1:-67179997 Msa0566160:intron
GTACCAACAGCATCTGCAGC+AGG 0.553748 4_1:-67178901 Msa0566160:CDS
ACGTTTAGAAAGTTTAGCAC+AGG 0.554685 4_1:-67179896 Msa0566160:CDS
AGGAGATCATCAAGCCATGA+TGG 0.559096 4_1:+67181106 None:intergenic
TCAAAACAACGCTACAGCTA+CGG 0.567053 4_1:+67178730 None:intergenic
ACTTCAGTACATAGCCGAGC+TGG 0.567891 4_1:-67180141 Msa0566160:CDS
TTATTTAATGTTGCGCCTGG+TGG 0.569735 4_1:-67178554 Msa0566160:three_prime_UTR
TCTGGTTCGTATAGTGTACA+TGG 0.570544 4_1:+67180770 None:intergenic
AAGTCTGGAGGTGACCAAGC+CGG 0.574482 4_1:+67180944 None:intergenic
ATTTGTTTCCTTTCGAAATG+CGG 0.576034 4_1:-67178685 Msa0566160:three_prime_UTR
AATCACGATTCATTAGAAGC+TGG 0.580917 4_1:-67180722 Msa0566160:CDS
GTGCGTCTATGGCTGCCAGA+TGG 0.582878 4_1:+67178858 None:intergenic
TTATCGAGGAGCAGCCATCA+TGG 0.585335 4_1:-67181120 Msa0566160:CDS
TCGGGGCATGACCCTGCGAA+CGG 0.588101 4_1:-67178774 Msa0566160:CDS
CCGGTTAATAAGCCACGAGA+AGG 0.591593 4_1:-67181181 Msa0566160:CDS
ACACAATCCCGCATTTCGAA+AGG 0.595218 4_1:+67178677 None:intergenic
ATCGAGTTACCACCTCCGGC+AGG 0.595939 4_1:+67178493 None:intergenic
GAAATTTGATGTATCCTGGA+AGG 0.596102 4_1:-67181297 Msa0566160:CDS
TCGTATAGTGTACATGGAGT+CGG 0.597809 4_1:+67180776 None:intergenic
ACTGAAGATTTCGGCAGGGG+CGG 0.598460 4_1:+67178457 None:intergenic
TCTGTCACTTGACCTGCCGG+AGG 0.600464 4_1:-67178505 Msa0566160:three_prime_UTR
AATGCAGGCAATTTGCTCAA+CGG 0.600746 4_1:-67180178 Msa0566160:intron
ACGCTGAAATAGCAGCTACA+CGG 0.602035 4_1:+67178622 None:intergenic
CTCGAATCTTCAATCTACAA+AGG 0.605158 4_1:-67182522 Msa0566160:five_prime_UTR
CAGCGTTAACTTGTAGACCA+AGG 0.605834 4_1:-67178219 Msa0566160:three_prime_UTR
TGCCGGAGGTGGTAACTCGA+TGG 0.610904 4_1:-67178491 Msa0566160:three_prime_UTR
CAATGGAGATTCACAAGAGG+AGG 0.613739 4_1:+67178417 None:intergenic
GCTACACGGGCAGAGAGAGA+GGG 0.614716 4_1:+67178636 None:intergenic
AACAAATTCTATCACAGGTG+TGG 0.615607 4_1:+67178701 None:intergenic
ATGACAATACATAGGCAGAA+GGG 0.618042 4_1:-67179996 Msa0566160:intron
AAAACAGAGGAATAGGTCAT+GGG 0.621223 4_1:-67180867 Msa0566160:CDS
TATGTCTCTAACCCTACAGT+TGG 0.625012 4_1:-67181211 Msa0566160:CDS
TGCGTCTAACATCAATTATG+CGG 0.625544 4_1:-67181002 Msa0566160:CDS
ATCTGAGAGCCATCTTATCG+AGG 0.627279 4_1:-67181134 Msa0566160:CDS
CATGACGAGCATCTTTGTTG+CGG 0.629591 4_1:+67180918 None:intergenic
TGCAAATAGCAGATATCTCG+GGG 0.631240 4_1:-67178376 Msa0566160:three_prime_UTR
ATAATCAGCATATGCTTGGG+AGG 0.639450 4_1:+67181231 None:intergenic
GGTTAGCAAACTGAGAGTCA+AGG 0.642753 4_1:+67178823 None:intergenic
CCAGATACACCTGTCAGAAG+GGG 0.643081 4_1:-67181076 Msa0566160:CDS
GATACACCTGTCAGAAGGGG+TGG 0.644099 4_1:-67181073 Msa0566160:CDS
TAACCTTTCTCTGAAGCACA+AGG 0.645943 4_1:-67178805 Msa0566160:CDS
AATCACTGAAGATTTCGGCA+GGG 0.647708 4_1:+67178453 None:intergenic
GGCTCTCAGATGAAGTACGT+TGG 0.650183 4_1:+67181146 None:intergenic
GTACACTATACGAACCAGAT+CGG 0.654234 4_1:-67180766 Msa0566160:CDS
TAGGCCAAATTCAAACACAA+TGG 0.655311 4_1:+67178400 None:intergenic
AAATCAATGCAGCAAAACAG+AGG 0.655642 4_1:-67180880 Msa0566160:CDS
TTGTTGCGGTCAAAGTCTGG+AGG 0.662715 4_1:+67180932 None:intergenic
ACACAATGGAGATTCACAAG+AGG 0.673796 4_1:+67178414 None:intergenic
AGAGAGATTGGAAGATTACG+TGG 0.686422 4_1:-67178927 Msa0566160:CDS
AGAGAACATTGAGACAACGA+AGG 0.693101 4_1:+67178598 None:intergenic
AGATTCGAGATCCCTAAAAG+GGG 0.695611 4_1:+67182536 None:intergenic
AGCACAAGGACACAAATTCG+GGG 0.749125 4_1:-67178791 Msa0566160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATCAAGAATAAAAAAA+AGG + chr4_1:67178342-67178361 None:intergenic 10.0%
!!! TTTAAAAATATATTTTTTGC+AGG + chr4_1:67178623-67178642 None:intergenic 10.0%
!! GAAAATTTAGATTAACAAAA+GGG + chr4_1:67178518-67178537 None:intergenic 15.0%
!! AGAAAATTTAGATTAACAAA+AGG + chr4_1:67178519-67178538 None:intergenic 15.0%
!! ATATTAAAGTTTCTATCTTT+GGG - chr4_1:67178580-67178599 Msa0566160:three_prime_UTR 15.0%
!!! ATTTTTTTTCTTATGTGAAT+TGG - chr4_1:67178653-67178672 Msa0566160:three_prime_UTR 15.0%
!! ATATAAGAATTTATCTATTC+CGG - chr4_1:67179858-67179877 Msa0566160:intron 15.0%
!! AGAAATTAGAATTAAGATTT+CGG + chr4_1:67182299-67182318 None:intergenic 15.0%
!! ATCAAGAATAAAAAAAAGGA+AGG + chr4_1:67178338-67178357 None:intergenic 20.0%
!! CATATTAAAGTTTCTATCTT+TGG - chr4_1:67178579-67178598 Msa0566160:three_prime_UTR 20.0%
!! TACATCAAACTTCAATTATT+TGG - chr4_1:67179193-67179212 Msa0566160:intron 20.0%
!!! CAATTATTTGGAACAATTAA+TGG - chr4_1:67179205-67179224 Msa0566160:intron 20.0%
!!! TTGTGGTTATTTTTTTGTAA+TGG - chr4_1:67179279-67179298 Msa0566160:intron 20.0%
!!! ATGCAATAAACAATTTTCAA+AGG + chr4_1:67179373-67179392 None:intergenic 20.0%
!!! TTTAAACAATCTTATTTGAC+AGG - chr4_1:67179454-67179473 Msa0566160:intron 20.0%
!!! AAAATCACCTTTTGAAAAAA+AGG - chr4_1:67180134-67180153 Msa0566160:CDS 20.0%
!!! TATCTGGAAAACTTTAAAAA+AGG + chr4_1:67180417-67180436 None:intergenic 20.0%
!! AACAAATTCAGTATTATACT+TGG - chr4_1:67180460-67180479 Msa0566160:intron 20.0%
!!! AAGAAATTAATGTTTTCTCT+TGG - chr4_1:67180574-67180593 Msa0566160:intron 20.0%
!! TTACATATATATACTGTATG+TGG - chr4_1:67180773-67180792 Msa0566160:CDS 20.0%
!!! TTCTTTTTATGACAATACAT+AGG - chr4_1:67180817-67180836 Msa0566160:CDS 20.0%
!!! TTTTTTACAAGAAATTCACA+AGG + chr4_1:67181112-67181131 None:intergenic 20.0%
!!! TAAATAGTTTAACTTAGAGA+AGG - chr4_1:67181331-67181350 Msa0566160:CDS 20.0%
!!! ATTCTGTTAAAACTTGTTTA+AGG - chr4_1:67181479-67181498 Msa0566160:intron 20.0%
!! ATATACAATTCAAAATTCAG+TGG - chr4_1:67181854-67181873 Msa0566160:intron 20.0%
!! TAAATAAATGGCAATAGTTT+AGG + chr4_1:67182254-67182273 None:intergenic 20.0%
!! AATGTGCATTATTATTTGTA+TGG - chr4_1:67182475-67182494 Msa0566160:five_prime_UTR 20.0%
!!! TTTTTAGGAAATAATCTTCT+CGG + chr4_1:67182575-67182594 None:intergenic 20.0%
!!! TTATTGATTTTCATTTTTGC+AGG - chr4_1:67182643-67182662 Msa0566160:five_prime_UTR 20.0%
!!! TATTGATTTTCATTTTTGCA+GGG - chr4_1:67182644-67182663 Msa0566160:five_prime_UTR 20.0%
!!! TTACACATTGCATCATTTTT+TGG - chr4_1:67178450-67178469 Msa0566160:three_prime_UTR 25.0%
! TTTGCTTATAATTCAGATAG+TGG - chr4_1:67178677-67178696 Msa0566160:three_prime_UTR 25.0%
!!! AAACCATCCTTTTTTTCAAA+AGG + chr4_1:67180144-67180163 None:intergenic 25.0%
!!! TTTCTGGTTTCTGTTTTTTT+TGG - chr4_1:67180225-67180244 Msa0566160:intron 25.0%
! TTCAGTATTATACTTGGAAA+AGG - chr4_1:67180466-67180485 Msa0566160:intron 25.0%
! TATAAAAACACTGTTAATGC+AGG - chr4_1:67180628-67180647 Msa0566160:intron 25.0%
! ATATATATACTGTATGTGGT+TGG - chr4_1:67180777-67180796 Msa0566160:CDS 25.0%
! CCATTACTTGAAAATGTAAA+TGG + chr4_1:67180993-67181012 None:intergenic 25.0%
!!! CCATTTACATTTTCAAGTAA+TGG - chr4_1:67180990-67181009 Msa0566160:CDS 25.0%
! TGATAGAAAACAAACACATT+AGG - chr4_1:67181173-67181192 Msa0566160:CDS 25.0%
!!! GTTTGATTTTAGCATATAAG+TGG - chr4_1:67181208-67181227 Msa0566160:CDS 25.0%
! CTGGAATGACATAAATTAAA+GGG - chr4_1:67181285-67181304 Msa0566160:CDS 25.0%
!!! TTGTCTCTATAACTTGTTTA+AGG - chr4_1:67181437-67181456 Msa0566160:intron 25.0%
!!! TGTCTCTATAACTTGTTTAA+GGG - chr4_1:67181438-67181457 Msa0566160:intron 25.0%
! TTTCTCATCGTTTATATGTT+TGG - chr4_1:67181592-67181611 Msa0566160:intron 25.0%
! AAATAAAACATAATTGGAGC+AGG + chr4_1:67181666-67181685 None:intergenic 25.0%
!!! TCTTGTCTAAGTCATTTTTA+TGG + chr4_1:67181721-67181740 None:intergenic 25.0%
!!! AATTCTCTCTAGTCTTTTTT+TGG - chr4_1:67181768-67181787 Msa0566160:intron 25.0%
! TACATAATCACTGAAGATTT+CGG + chr4_1:67182376-67182395 None:intergenic 25.0%
!!! TAACGCTGAACTTATTTTTT+AGG + chr4_1:67182590-67182609 None:intergenic 25.0%
! TGTGAATTTTCTCTCTCTTA+GGG + chr4_1:67178246-67178265 None:intergenic 30.0%
! TTGTGAATTTTCTCTCTCTT+AGG + chr4_1:67178247-67178266 None:intergenic 30.0%
TGATGCAATGTGTAACTAAA+TGG + chr4_1:67178445-67178464 None:intergenic 30.0%
AACAAAAGGGTCAACAAAAA+GGG + chr4_1:67178505-67178524 None:intergenic 30.0%
TAACAAAAGGGTCAACAAAA+AGG + chr4_1:67178506-67178525 None:intergenic 30.0%
! AAATTTTCTGAACGCTTTTG+TGG - chr4_1:67178530-67178549 Msa0566160:three_prime_UTR 30.0%
! TGTTGAGTTTTCTGTTACAT+TGG - chr4_1:67178762-67178781 Msa0566160:CDS 30.0%
!!! CAAACTGTTGTTTTCTTGAT+AGG - chr4_1:67178788-67178807 Msa0566160:CDS 30.0%
TGTTTCGTACTATTTCATAC+CGG - chr4_1:67178955-67178974 Msa0566160:intron 30.0%
!! TTTTCAAGAGCATAGTGTTA+TGG - chr4_1:67179128-67179147 Msa0566160:intron 30.0%
ACTCGTACTAAGATCTTATA+AGG + chr4_1:67179242-67179261 None:intergenic 30.0%
GTTTAAGTATCATGAGTTTG+TGG - chr4_1:67179262-67179281 Msa0566160:intron 30.0%
TCTTTAATGCACTTACTACT+TGG - chr4_1:67179415-67179434 Msa0566160:intron 30.0%
GTTTAAATCACCAAACACTT+TGG + chr4_1:67179441-67179460 None:intergenic 30.0%
!!! GACTCTTTTTCAAATATGAG+TGG - chr4_1:67179976-67179995 Msa0566160:CDS 30.0%
!! TCACCTTTTGAAAAAAAGGA+TGG - chr4_1:67180138-67180157 Msa0566160:CDS 30.0%
TCTACCACATTTATATGACT+TGG - chr4_1:67180269-67180288 Msa0566160:intron 30.0%
TAAGAGTTAGAGAAACAAGT+AGG + chr4_1:67180549-67180568 None:intergenic 30.0%
!! TAATGGATGTCTGTTTTACT+GGG - chr4_1:67181007-67181026 Msa0566160:CDS 30.0%
AAATGCAACACAAATTCACA+AGG + chr4_1:67181140-67181159 None:intergenic 30.0%
GCTGGAATGACATAAATTAA+AGG - chr4_1:67181284-67181303 Msa0566160:CDS 30.0%
GAATGACATAAATTAAAGGG+AGG - chr4_1:67181288-67181307 Msa0566160:CDS 30.0%
! TCTACCTTGAGCTTAAATTT+TGG + chr4_1:67181358-67181377 None:intergenic 30.0%
TTAAGGAATATCTCATTGTC+TGG - chr4_1:67181496-67181515 Msa0566160:intron 30.0%
TAAGGAATATCTCATTGTCT+GGG - chr4_1:67181497-67181516 Msa0566160:intron 30.0%
AAAAGACTAGAGAGAATTCA+AGG + chr4_1:67181766-67181785 None:intergenic 30.0%
!! TTAGGAACGATTAGTTTCAT+CGG - chr4_1:67181806-67181825 Msa0566160:intron 30.0%
AAGGAAACAAATTCTATCAC+AGG + chr4_1:67182128-67182147 None:intergenic 30.0%
ATTTGTTTCCTTTCGAAATG+CGG - chr4_1:67182136-67182155 Msa0566160:intron 30.0%
ATCTCCATTGTGTTTGAATT+TGG - chr4_1:67182417-67182436 Msa0566160:five_prime_UTR 30.0%
! AGATATCTGCTATTTGCATT+AGG + chr4_1:67182443-67182462 None:intergenic 30.0%
AATGCAAATAGCAGATATCT+CGG - chr4_1:67182443-67182462 Msa0566160:five_prime_UTR 30.0%
!! TTTGATTTCAAGCCCCTTTT+AGG - chr4_1:67178273-67178292 Msa0566160:three_prime_UTR 35.0%
! TTGATTTCAAGCCCCTTTTA+GGG - chr4_1:67178274-67178293 Msa0566160:three_prime_UTR 35.0%
AAGATTCGAGATCCCTAAAA+GGG + chr4_1:67178289-67178308 None:intergenic 35.0%
CTCGAATCTTCAATCTACAA+AGG - chr4_1:67178299-67178318 Msa0566160:three_prime_UTR 35.0%
ACAAAAGGGTCAACAAAAAG+GGG + chr4_1:67178504-67178523 None:intergenic 35.0%
ATCGAAGATTCTTATGTGTG+AGG - chr4_1:67178922-67178941 Msa0566160:CDS 35.0%
AGATTCTTATGTGTGAGGAT+AGG - chr4_1:67178927-67178946 Msa0566160:CDS 35.0%
TCATGACAAGAATGCAAATC+CGG + chr4_1:67178977-67178996 None:intergenic 35.0%
ATTCTTGTCATGAAAATGCG+TGG - chr4_1:67178984-67179003 Msa0566160:intron 35.0%
GAAAATTGCCCATGTGTAAA+CGG + chr4_1:67179036-67179055 None:intergenic 35.0%
AGCTGAGAATTGTTGCAATT+TGG - chr4_1:67179318-67179337 Msa0566160:intron 35.0%
GAGAATTGTTGCAATTTGGA+TGG - chr4_1:67179322-67179341 Msa0566160:intron 35.0%
GTCAGAAATTTGATGTATCC+TGG - chr4_1:67179520-67179539 Msa0566160:intron 35.0%
GAAATTTGATGTATCCTGGA+AGG - chr4_1:67179524-67179543 Msa0566160:intron 35.0%
GACATAATCAGCATATGCTT+GGG + chr4_1:67179596-67179615 None:intergenic 35.0%
AGACATAATCAGCATATGCT+TGG + chr4_1:67179597-67179616 None:intergenic 35.0%
TGCGTCTAACATCAATTATG+CGG - chr4_1:67179819-67179838 Msa0566160:intron 35.0%
AGAATTTATCTATTCCGGCT+TGG - chr4_1:67179863-67179882 Msa0566160:intron 35.0%
!! GCATTGATTTCTGCATACAT+AGG + chr4_1:67179931-67179950 None:intergenic 35.0%
AAATCAATGCAGCAAAACAG+AGG - chr4_1:67179941-67179960 Msa0566160:CDS 35.0%
AAAACAGAGGAATAGGTCAT+GGG - chr4_1:67179954-67179973 Msa0566160:CDS 35.0%
!!! AATGTTGCTTTTCAGAGATC+AGG - chr4_1:67180018-67180037 Msa0566160:intron 35.0%
! GATTTTCATTTGCAGTCAGT+AGG + chr4_1:67180083-67180102 None:intergenic 35.0%
AATCACGATTCATTAGAAGC+TGG - chr4_1:67180099-67180118 Msa0566160:intron 35.0%
! ACAGTATGCAGTTTCTTTTC+TGG - chr4_1:67180209-67180228 Msa0566160:intron 35.0%
! ACCACATTTATATGACTTGG+AGG - chr4_1:67180272-67180291 Msa0566160:intron 35.0%
CTATGTGAAATCATTCTGCA+TGG + chr4_1:67180359-67180378 None:intergenic 35.0%
TCAATCATATGCAGTGTATC+TGG + chr4_1:67180433-67180452 None:intergenic 35.0%
TAGGAATAGTATACGAGCTT+TGG + chr4_1:67180514-67180533 None:intergenic 35.0%
AAGTAGGCTCTATTCTACAT+AGG + chr4_1:67180533-67180552 None:intergenic 35.0%
AAGAAATGTACAGGCTTTAC+AGG - chr4_1:67180704-67180723 Msa0566160:CDS 35.0%
TGCATAATATTGCTCTATGG+TGG + chr4_1:67180736-67180755 None:intergenic 35.0%
CTCTGCATAATATTGCTCTA+TGG + chr4_1:67180739-67180758 None:intergenic 35.0%
TATGACAATACATAGGCAGA+AGG - chr4_1:67180824-67180843 Msa0566160:CDS 35.0%
ATGACAATACATAGGCAGAA+GGG - chr4_1:67180825-67180844 Msa0566160:CDS 35.0%
ACGTTTAGAAAGTTTAGCAC+AGG - chr4_1:67180925-67180944 Msa0566160:CDS 35.0%
!!! GTAATGGATGTCTGTTTTAC+TGG - chr4_1:67181006-67181025 Msa0566160:CDS 35.0%
! TTTACTGGGAAATGTCGATT+TGG - chr4_1:67181021-67181040 Msa0566160:CDS 35.0%
!! TTACTGGGAAATGTCGATTT+GGG - chr4_1:67181022-67181041 Msa0566160:CDS 35.0%
TTTATGTCATTCCAGCAATC+AGG + chr4_1:67181280-67181299 None:intergenic 35.0%
AGGACCAAAATTTAAGCTCA+AGG - chr4_1:67181351-67181370 Msa0566160:intron 35.0%
TTAAGGGATATCTCATTGTC+TGG - chr4_1:67181454-67181473 Msa0566160:intron 35.0%
TAAGGGATATCTCATTGTCT+GGG - chr4_1:67181455-67181474 Msa0566160:intron 35.0%
GTTCCTAAAAAGAACTTGCA+TGG + chr4_1:67181794-67181813 None:intergenic 35.0%
AATTCAAAATTCAGTGGAGC+AGG - chr4_1:67181860-67181879 Msa0566160:intron 35.0%
AACAAATTCTATCACAGGTG+TGG + chr4_1:67182123-67182142 None:intergenic 35.0%
TTTGTTTCCTTTCGAAATGC+GGG - chr4_1:67182137-67182156 Msa0566160:intron 35.0%
CAGGCGCAACATTAAATAAA+TGG + chr4_1:67182266-67182285 None:intergenic 35.0%
CATTTATTTAATGTTGCGCC+TGG - chr4_1:67182264-67182283 Msa0566160:intron 35.0%
TAGGCCAAATTCAAACACAA+TGG + chr4_1:67182424-67182443 None:intergenic 35.0%
ATGCAAATAGCAGATATCTC+GGG - chr4_1:67182444-67182463 Msa0566160:five_prime_UTR 35.0%
AGATTCGAGATCCCTAAAAG+GGG + chr4_1:67178288-67178307 None:intergenic 40.0%
GAAGATTCGAGATCCCTAAA+AGG + chr4_1:67178290-67178309 None:intergenic 40.0%
! TTTTGTGGTTGTGCATGCTT+TGG - chr4_1:67178545-67178564 Msa0566160:three_prime_UTR 40.0%
TTTGTGGTTGTGCATGCTTT+GGG - chr4_1:67178546-67178565 Msa0566160:three_prime_UTR 40.0%
! TGCATGCTTTGGGTAGATTA+TGG - chr4_1:67178556-67178575 Msa0566160:three_prime_UTR 40.0%
!! TCTTTGGGTTTTTCCTCATC+TGG - chr4_1:67178595-67178614 Msa0566160:three_prime_UTR 40.0%
!! CTTTGGGTTTTTCCTCATCT+GGG - chr4_1:67178596-67178615 Msa0566160:three_prime_UTR 40.0%
TTGTTGCAATTTGGATGGCA+TGG - chr4_1:67179327-67179346 Msa0566160:intron 40.0%
! TACTACTTGGCCAAAGTGTT+TGG - chr4_1:67179428-67179447 Msa0566160:intron 40.0%
AGGTATTCATCCTGCTTCTA+TGG - chr4_1:67179474-67179493 Msa0566160:intron 40.0%
TTTGAATTCGCCATAGAAGC+AGG + chr4_1:67179487-67179506 None:intergenic 40.0%
!! GTACTAGGAAATGGGCTTTT+GGG + chr4_1:67179571-67179590 None:intergenic 40.0%
ATAATCAGCATATGCTTGGG+AGG + chr4_1:67179593-67179612 None:intergenic 40.0%
TATGTCTCTAACCCTACAGT+TGG - chr4_1:67179610-67179629 Msa0566160:intron 40.0%
ATGCAGCAAAACAGAGGAAT+AGG - chr4_1:67179947-67179966 Msa0566160:CDS 40.0%
CAAAACAGAGGAATAGGTCA+TGG - chr4_1:67179953-67179972 Msa0566160:CDS 40.0%
AATATGAGTGGTGTTCCATC+TGG - chr4_1:67179988-67180007 Msa0566160:intron 40.0%
TCGTATAGTGTACATGGAGT+CGG + chr4_1:67180048-67180067 None:intergenic 40.0%
! TCTGGTTCGTATAGTGTACA+TGG + chr4_1:67180054-67180073 None:intergenic 40.0%
GTACACTATACGAACCAGAT+CGG - chr4_1:67180055-67180074 Msa0566160:intron 40.0%
GCCTCCAAGTCATATAAATG+TGG + chr4_1:67180276-67180295 None:intergenic 40.0%
! CATTTATATGACTTGGAGGC+TGG - chr4_1:67180276-67180295 Msa0566160:intron 40.0%
! AATGCAGGCAATTTGCTCAA+CGG - chr4_1:67180643-67180662 Msa0566160:CDS 40.0%
CGGCTATGTACTGAAGTTTA+CGG + chr4_1:67180677-67180696 None:intergenic 40.0%
CTGTACATTTCTTTCCAGCT+CGG + chr4_1:67180697-67180716 None:intergenic 40.0%
TACTCAGGATAAGATTCTGC+TGG + chr4_1:67180883-67180902 None:intergenic 40.0%
GCAGAATCTTATCCTGAGTA+TGG - chr4_1:67180883-67180902 Msa0566160:CDS 40.0%
! GTGCTTTATTCTCCATACTC+AGG + chr4_1:67180898-67180917 None:intergenic 40.0%
ATGAACTTTAGCCTGATTGC+TGG - chr4_1:67181266-67181285 Msa0566160:CDS 40.0%
ACTCGAGTATGCACAGATTA+TGG + chr4_1:67181546-67181565 None:intergenic 40.0%
AGACTAGAGAGAATTCAAGG+AGG + chr4_1:67181763-67181782 None:intergenic 40.0%
! TGGCCATGCAAGTTCTTTTT+AGG - chr4_1:67181788-67181807 Msa0566160:intron 40.0%
AGAGAGATTGGAAGATTACG+TGG - chr4_1:67181894-67181913 Msa0566160:intron 40.0%
!! TAACCTTTCTCTGAAGCACA+AGG - chr4_1:67182016-67182035 Msa0566160:intron 40.0%
GAAGCACAAGGACACAAATT+CGG - chr4_1:67182028-67182047 Msa0566160:intron 40.0%
AAGCACAAGGACACAAATTC+GGG - chr4_1:67182029-67182048 Msa0566160:intron 40.0%
TCAAAACAACGCTACAGCTA+CGG + chr4_1:67182094-67182113 None:intergenic 40.0%
AGAGAACATTGAGACAACGA+AGG + chr4_1:67182226-67182245 None:intergenic 40.0%
TTATTTAATGTTGCGCCTGG+TGG - chr4_1:67182267-67182286 Msa0566160:intron 40.0%
AATCACTGAAGATTTCGGCA+GGG + chr4_1:67182371-67182390 None:intergenic 40.0%
TAATCACTGAAGATTTCGGC+AGG + chr4_1:67182372-67182391 None:intergenic 40.0%
ACACAATGGAGATTCACAAG+AGG + chr4_1:67182410-67182429 None:intergenic 40.0%
TGCAAATAGCAGATATCTCG+GGG - chr4_1:67182445-67182464 Msa0566160:five_prime_UTR 40.0%
CTCTTCTTGTTGAAGCTGTG+TGG + chr4_1:67178195-67178214 None:intergenic 45.0%
GCTTCAACAAGAAGAGAGAC+AGG - chr4_1:67178199-67178218 Msa0566160:three_prime_UTR 45.0%
!!! TTTTTTGCAGGACCCAGATG+AGG + chr4_1:67178611-67178630 None:intergenic 45.0%
TTGCCCATGTGTAAACGGTA+CGG + chr4_1:67179031-67179050 None:intergenic 45.0%
GGCGAATTCAAAGAGCTCAT+CGG - chr4_1:67179495-67179514 Msa0566160:intron 45.0%
GCGAATTCAAAGAGCTCATC+GGG - chr4_1:67179496-67179515 Msa0566160:intron 45.0%
!! GGTACTAGGAAATGGGCTTT+TGG + chr4_1:67179572-67179591 None:intergenic 45.0%
!! CTTGGGAGGTACTAGGAAAT+GGG + chr4_1:67179579-67179598 None:intergenic 45.0%
! GTGGCTTATTAACCGGCTTT+GGG + chr4_1:67179636-67179655 None:intergenic 45.0%
CCTTCTCGTGGCTTATTAAC+CGG + chr4_1:67179643-67179662 None:intergenic 45.0%
TGATGTGAGTTTCCTTCTCG+TGG + chr4_1:67179655-67179674 None:intergenic 45.0%
ATCTGAGAGCCATCTTATCG+AGG - chr4_1:67179687-67179706 Msa0566160:intron 45.0%
AGGAGATCATCAAGCCATGA+TGG + chr4_1:67179718-67179737 None:intergenic 45.0%
! CAGGTGTATCTGGCTCATTT+AGG + chr4_1:67179738-67179757 None:intergenic 45.0%
TCTTTGTTGCGGTCAAAGTC+TGG + chr4_1:67179895-67179914 None:intergenic 45.0%
CATGACGAGCATCTTTGTTG+CGG + chr4_1:67179906-67179925 None:intergenic 45.0%
GCAACATTGTCTTTGCCAGA+TGG + chr4_1:67180006-67180025 None:intergenic 45.0%
CGAGCTGGAAAGAAATGTAC+AGG - chr4_1:67180695-67180714 Msa0566160:CDS 45.0%
TTTGGGTACTCTGCATGTCA+AGG - chr4_1:67181039-67181058 Msa0566160:CDS 45.0%
TTGGGTACTCTGCATGTCAA+GGG - chr4_1:67181040-67181059 Msa0566160:CDS 45.0%
AGAGAGAATTCAAGGAGGCA+GGG + chr4_1:67181758-67181777 None:intergenic 45.0%
TAGAGAGAATTCAAGGAGGC+AGG + chr4_1:67181759-67181778 None:intergenic 45.0%
!! GAAGTACTGGAGAGAGAGAT+TGG - chr4_1:67181882-67181901 Msa0566160:intron 45.0%
GGTTAGCAAACTGAGAGTCA+AGG + chr4_1:67182001-67182020 None:intergenic 45.0%
TGTCCTTGTGCTTCAGAGAA+AGG + chr4_1:67182022-67182041 None:intergenic 45.0%
AGCACAAGGACACAAATTCG+GGG - chr4_1:67182030-67182049 Msa0566160:intron 45.0%
ACACAATCCCGCATTTCGAA+AGG + chr4_1:67182147-67182166 None:intergenic 45.0%
GGGATTGTGTAGAGATTTCC+AGG - chr4_1:67182157-67182176 Msa0566160:intron 45.0%
ACGCTGAAATAGCAGCTACA+CGG + chr4_1:67182202-67182221 None:intergenic 45.0%
! ATTTCTGTCACTTGACCTGC+CGG - chr4_1:67182313-67182332 Msa0566160:intron 45.0%
ATCACTGAAGATTTCGGCAG+GGG + chr4_1:67182370-67182389 None:intergenic 45.0%
CAATGGAGATTCACAAGAGG+AGG + chr4_1:67182407-67182426 None:intergenic 45.0%
!! CAGCGTTAACTTGTAGACCA+AGG - chr4_1:67182602-67182621 Msa0566160:five_prime_UTR 45.0%
!! CCTCTAAAGCTTTCAAGCCT+TGG + chr4_1:67182622-67182641 None:intergenic 45.0%
! CCAAGGCTTGAAAGCTTTAG+AGG - chr4_1:67182619-67182638 Msa0566160:five_prime_UTR 45.0%
!! TTTATTAAATAAAACATAAT+TGG + chr4_1:67181672-67181691 None:intergenic 5.0%
CCACCGTACCGTTTACACAT+GGG - chr4_1:67179025-67179044 Msa0566160:intron 50.0%
!! CTAGGAAATGGGCTTTTGGG+AGG + chr4_1:67179568-67179587 None:intergenic 50.0%
!! GCTTGGGAGGTACTAGGAAA+TGG + chr4_1:67179580-67179599 None:intergenic 50.0%
!! GCATATGCTTGGGAGGTACT+AGG + chr4_1:67179586-67179605 None:intergenic 50.0%
! CGTGGCTTATTAACCGGCTT+TGG + chr4_1:67179637-67179656 None:intergenic 50.0%
CCGGTTAATAAGCCACGAGA+AGG - chr4_1:67179640-67179659 Msa0566160:intron 50.0%
GGCTCTCAGATGAAGTACGT+TGG + chr4_1:67179678-67179697 None:intergenic 50.0%
ATGGCTGCTCCTCGATAAGA+TGG + chr4_1:67179699-67179718 None:intergenic 50.0%
TTATCGAGGAGCAGCCATCA+TGG - chr4_1:67179701-67179720 Msa0566160:intron 50.0%
AGCCAGATACACCTGTCAGA+AGG - chr4_1:67179743-67179762 Msa0566160:intron 50.0%
GCCAGATACACCTGTCAGAA+GGG - chr4_1:67179744-67179763 Msa0566160:intron 50.0%
CCAGATACACCTGTCAGAAG+GGG - chr4_1:67179745-67179764 Msa0566160:intron 50.0%
TTGTTGCGGTCAAAGTCTGG+AGG + chr4_1:67179892-67179911 None:intergenic 50.0%
! GACTTGGAGGCTGGATGTTT+TGG - chr4_1:67180285-67180304 Msa0566160:intron 50.0%
! GGCAATTTGCTCAACGGTCA+CGG - chr4_1:67180649-67180668 Msa0566160:CDS 50.0%
! GCAATTTGCTCAACGGTCAC+GGG - chr4_1:67180650-67180669 Msa0566160:CDS 50.0%
ACTTCAGTACATAGCCGAGC+TGG - chr4_1:67180680-67180699 Msa0566160:CDS 50.0%
! GGCAGAAGGGTCTGAAGTAT+CGG - chr4_1:67180838-67180857 Msa0566160:CDS 50.0%
TATGCTTGCATACCAAGCGC+TGG - chr4_1:67181081-67181100 Msa0566160:CDS 50.0%
CACAAGGATTTACCAGCGCT+TGG + chr4_1:67181096-67181115 None:intergenic 50.0%
! TTCAGTGGAGCAGGAAGTAC+TGG - chr4_1:67181869-67181888 Msa0566160:intron 50.0%
CAGCCTCAACAACAACCATC+TGG - chr4_1:67181948-67181967 Msa0566160:intron 50.0%
! CTGCCAGATGGTTGTTGTTG+AGG + chr4_1:67181954-67181973 None:intergenic 50.0%
! CTCTGCTGTTTGTGCGTCTA+TGG + chr4_1:67181977-67181996 None:intergenic 50.0%
CGCTGAAATAGCAGCTACAC+GGG + chr4_1:67182201-67182220 None:intergenic 50.0%
AGATTTCGGCAATGACCACC+AGG + chr4_1:67182285-67182304 None:intergenic 50.0%
AACCATCGAGTTACCACCTC+CGG + chr4_1:67182335-67182354 None:intergenic 50.0%
GCCACCGTACCGTTTACACA+TGG - chr4_1:67179024-67179043 Msa0566160:intron 55.0%
CCCATGTGTAAACGGTACGG+TGG + chr4_1:67179028-67179047 None:intergenic 55.0%
AGAGCATGTGCATGCCTTCC+AGG + chr4_1:67179541-67179560 None:intergenic 55.0%
CCCCTTCTGACAGGTGTATC+TGG + chr4_1:67179748-67179767 None:intergenic 55.0%
GATACACCTGTCAGAAGGGG+TGG - chr4_1:67179748-67179767 Msa0566160:intron 55.0%
CTATGACCACCCCTTCTGAC+AGG + chr4_1:67179757-67179776 None:intergenic 55.0%
CTGTCAGAAGGGGTGGTCAT+AGG - chr4_1:67179755-67179774 Msa0566160:intron 55.0%
AAGTCTGGAGGTGACCAAGC+CGG + chr4_1:67179880-67179899 None:intergenic 55.0%
GCAGTCAGTAGGATCCGATC+TGG + chr4_1:67180072-67180091 None:intergenic 55.0%
GTACCAACAGCATCTGCAGC+AGG - chr4_1:67181920-67181939 Msa0566160:intron 55.0%
TTCTGAGTGCTCCGTTCGCA+GGG + chr4_1:67182061-67182080 None:intergenic 55.0%
ATTCTGAGTGCTCCGTTCGC+AGG + chr4_1:67182062-67182081 None:intergenic 55.0%
! CAGAGAGAGAGGGCATATCC+TGG + chr4_1:67182178-67182197 None:intergenic 55.0%
ACTGAAGATTTCGGCAGGGG+CGG + chr4_1:67182367-67182386 None:intergenic 55.0%
!! CCGGCTTTGGGCCAACTGTA+GGG + chr4_1:67179624-67179643 None:intergenic 60.0%
CCCTACAGTTGGCCCAAAGC+CGG - chr4_1:67179621-67179640 Msa0566160:intron 60.0%
!! ACCGGCTTTGGGCCAACTGT+AGG + chr4_1:67179625-67179644 None:intergenic 60.0%
GTGCCTGCTGCAGATGCTGT+TGG + chr4_1:67181926-67181945 None:intergenic 60.0%
GTGCGTCTATGGCTGCCAGA+TGG + chr4_1:67181966-67181985 None:intergenic 60.0%
GCTACACGGGCAGAGAGAGA+GGG + chr4_1:67182188-67182207 None:intergenic 60.0%
AGCTACACGGGCAGAGAGAG+AGG + chr4_1:67182189-67182208 None:intergenic 60.0%
! TCTGTCACTTGACCTGCCGG+AGG - chr4_1:67182316-67182335 Msa0566160:intron 60.0%
ATCGAGTTACCACCTCCGGC+AGG + chr4_1:67182331-67182350 None:intergenic 60.0%
TGCCGGAGGTGGTAACTCGA+TGG - chr4_1:67182330-67182349 Msa0566160:intron 60.0%
TCGGGGCATGACCCTGCGAA+CGG - chr4_1:67182047-67182066 Msa0566160:intron 65.0%
GTCACTTGACCTGCCGGAGG+TGG - chr4_1:67182319-67182338 Msa0566160:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 67178158 67182685 67178158 ID=Msa0566160;Name=Msa0566160
chr4_1 mRNA 67178158 67182685 67178158 ID=Msa0566160-mRNA-1;Parent=Msa0566160;Name=Msa0566160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=347|1|1|1|0.75|0.6|5|600|377
chr4_1 exon 67178158 67178969 67178158 ID=Msa0566160-mRNA-1:exon:21950;Parent=Msa0566160-mRNA-1
chr4_1 exon 67179878 67180004 67179878 ID=Msa0566160-mRNA-1:exon:21949;Parent=Msa0566160-mRNA-1
chr4_1 exon 67180118 67180193 67180118 ID=Msa0566160-mRNA-1:exon:21948;Parent=Msa0566160-mRNA-1
chr4_1 exon 67180632 67181367 67180632 ID=Msa0566160-mRNA-1:exon:21947;Parent=Msa0566160-mRNA-1
chr4_1 exon 67182356 67182685 67182356 ID=Msa0566160-mRNA-1:exon:21946;Parent=Msa0566160-mRNA-1
chr4_1 five_prime_UTR 67182356 67182685 67182356 ID=Msa0566160-mRNA-1:five_prime_utr;Parent=Msa0566160-mRNA-1
chr4_1 five_prime_UTR 67181351 67181367 67181351 ID=Msa0566160-mRNA-1:five_prime_utr;Parent=Msa0566160-mRNA-1
chr4_1 CDS 67180632 67181350 67180632 ID=Msa0566160-mRNA-1:cds;Parent=Msa0566160-mRNA-1
chr4_1 CDS 67180118 67180193 67180118 ID=Msa0566160-mRNA-1:cds;Parent=Msa0566160-mRNA-1
chr4_1 CDS 67179878 67180004 67179878 ID=Msa0566160-mRNA-1:cds;Parent=Msa0566160-mRNA-1
chr4_1 CDS 67178758 67178969 67178758 ID=Msa0566160-mRNA-1:cds;Parent=Msa0566160-mRNA-1
chr4_1 three_prime_UTR 67178158 67178757 67178158 ID=Msa0566160-mRNA-1:three_prime_utr;Parent=Msa0566160-mRNA-1
Gene Sequence

>Msa0566160

ATGGCGAATTCAAAGAGCTCATCGGGTGTCAGAAATTTGATGTATCCTGGAAGGCATGCACATGCTCTGCTTCCTCCCAAAAGCCCATTTCCTAGTACCTCCCAAGCATATGCTGATTATGTCTCTAACCCTACAGTTGGCCCAAAGCCGGTTAATAAGCCACGAGAAGGAAACTCACATCACCAACGTACTTCATCTGAGAGCCATCTTATCGAGGAGCAGCCATCATGGCTTGATGATCTCCTAAATGAGCCAGATACACCTGTCAGAAGGGGTGGTCATAGGCGTTCATCAAGTGATTCTTTTGCATATATTGACAATATTAATGCGTCTAACATCAATTATGCGGATCATGATGAGTATAAATATAAGAATTTATCTATTCCGGCTTGGTCACCTCCAGACTTTGACCGCAACAAAGATGCTCGTCATGTACCTATGTATGCAGAAATCAATGCAGCAAAACAGAGGAATAGGTCATGGGACTCTTTTTCAAATATGAGTGGTGTTCCATCTGGCAAAGACAATGTTGCTTTTCAGAGATCAGGATCACCGACTCCATGTACACTATACGAACCAGATCGGATCCTACTGACTGCAAATGAAAATCACGATTCATTAGAAGCTGGCATACAAGACACAAAATCACCTTTTGAAAAAAAGGATGGTTTACATGCAAAGTCATCTGCTTCTGAAACAGATTCAAAACGTGCTAAACAGCAATTTGCTCAACGGTCACGGGTCCGTAAACTTCAGTACATAGCCGAGCTGGAAAGAAATGTACAGGCTTTACAGGCAGAAGGGTCTGAAGTATCGGCTGAGCTTGAGTTTCTTAACCAGCAGAATCTTATCCTGAGTATGGAGAATAAAGCACTCAAACAACGTTTAGAAAGTTTAGCACAGGAGCAGCTTATCAAATATCTGGAGCAGGAAGTACTGGAGAGAGAGATTGGAAGATTACGTGGCATGTACCAACAGCATCTGCAGCAGGCACAGCAGCCTCAACAACAACCATCTGGCAGCCATAGACGCACAAACAGCAGAGACCTTGACTCTCAGTTTGCTAACCTTTCTCTGAAGCACAAGGACACAAATTCGGGGCATGACCCTGCGAACGGAGCACTCAGAATTTAG
Protein sequence

>Msa0566160

MANSKSSSGVRNLMYPGRHAHALLPPKSPFPSTSQAYADYVSNPTVGPKPVNKPREGNSHHQRTSSESHLIEEQPSWLDDLLNEPDTPVRRGGHRRSSSDSFAYIDNINASNINYADHDEYKYKNLSIPAWSPPDFDRNKDARHVPMYAEINAAKQRNRSWDSFSNMSGVPSGKDNVAFQRSGSPTPCTLYEPDRILLTANENHDSLEAGIQDTKSPFEKKDGLHAKSSASETDSKRAKQQFAQRSRVRKLQYIAELERNVQALQAEGSEVSAELEFLNQQNLILSMENKALKQRLESLAQEQLIKYLEQEVLEREIGRLRGMYQQHLQQAQQPQQQPSGSHRRTNSRDLDSQFANLSLKHKDTNSGHDPANGALRI*