AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0269810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0269810 MtrunA17_Chr2g0319481 93.182 220 11 1 1 220 1 216 9.71e-149 412
Msa0269810 MtrunA17_Chr4g0041881 46.698 212 102 5 8 210 11 220 2.12e-58 184
Msa0269810 MtrunA17_Chr1g0206161 23.699 173 116 4 10 175 52 215 6.11e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0269810 AT3G20600.1 41.905 210 114 5 8 213 13 218 5.00e-47 155
Msa0269810 AT3G20590.1 42.857 182 96 4 9 185 14 192 1.61e-34 124
Msa0269810 AT3G20610.1 35.323 201 116 7 22 215 26 219 3.21e-29 109
Msa0269810 AT4G05220.1 22.159 176 122 4 13 180 47 215 2.46e-11 61.6

Find 61 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATACCTTTATCTTTATT+AGG 0.156001 2_3:-843220 None:intergenic
TGCGGTTAAGTATAAGATTT+TGG 0.193625 2_3:+843450 Msa0269810:CDS
TGGAGATTCTGGGGAGAAAA+TGG 0.196345 2_3:+843522 Msa0269810:CDS
CTTGGTAATGCTACTGTAAA+TGG 0.197197 2_3:+843301 Msa0269810:CDS
CATCATAACCATAGGCTTAA+TGG 0.228595 2_3:+843057 Msa0269810:CDS
TGATCTTGCTGTTGTGTTTA+GGG 0.236469 2_3:-843161 None:intergenic
AATGTGGAAATTGGAGATTC+TGG 0.246829 2_3:+843511 Msa0269810:CDS
GAATACCTTTATCTTTATTA+GGG 0.259706 2_3:-843219 None:intergenic
TTGATCTTGCTGTTGTGTTT+AGG 0.268959 2_3:-843162 None:intergenic
ATCGTGCGCTTCTTGTGTTT+TGG 0.270001 2_3:+843620 Msa0269810:CDS
TTGATGGTAAAGTTTATCTA+AGG 0.284290 2_3:+843413 Msa0269810:CDS
GGAGGGGCAAATGTGGAAAT+TGG 0.299189 2_3:+843502 Msa0269810:CDS
AAGGTGAAGTTAAGATGAAT+AGG 0.325470 2_3:-843250 None:intergenic
GTCTTGTTTATCATTGGTCT+TGG 0.334988 2_3:+843643 Msa0269810:CDS
AGCTGTTGTTCATGCTGTTC+AGG 0.351132 2_3:+843031 Msa0269810:CDS
AATTTAACTGCGAGTGTTAA+GGG 0.363654 2_3:+843385 Msa0269810:CDS
GTATGCTTAGCCATATGAAT+AGG 0.380130 2_3:-843081 None:intergenic
GTTAACCCTAATAAAGATAA+AGG 0.389560 2_3:+843214 Msa0269810:CDS
GAATTTAACTGCGAGTGTTA+AGG 0.395995 2_3:+843384 Msa0269810:CDS
AGTGTTGAGAGTATTGTAAA+AGG 0.426836 2_3:-843269 None:intergenic
ACTGTAAATGGATTCTATCA+AGG 0.430123 2_3:+843313 Msa0269810:CDS
GGAGATTCTGGGGAGAAAAT+GGG 0.447531 2_3:+843523 Msa0269810:CDS
AAAGAGATCGGGTATGGGGA+GGG 0.451284 2_3:+843485 Msa0269810:CDS
AGTGTTAAGGGTGTTGTTGA+TGG 0.454047 2_3:+843397 Msa0269810:CDS
GCTTAATGGCCCTATTCATA+TGG 0.461217 2_3:+843071 Msa0269810:CDS
ATGTGGAAATTGGAGATTCT+GGG 0.462099 2_3:+843512 Msa0269810:CDS
AGGTGAAGTTAAGATGAATA+GGG 0.473108 2_3:-843249 None:intergenic
GGTATGGGGAGGGGCAAATG+TGG 0.473413 2_3:+843495 Msa0269810:CDS
TATGCTTAGCCATATGAATA+GGG 0.474917 2_3:-843080 None:intergenic
AAGAAAAGTGTGAGGCTTGG+AGG 0.475890 2_3:+843547 Msa0269810:CDS
CAGCATGAACAACAGCTGTT+GGG 0.497130 2_3:-843025 None:intergenic
GAATAGGGCCATTAAGCCTA+TGG 0.501191 2_3:-843065 None:intergenic
ATACGAAAAGAGATCGGGTA+TGG 0.508627 2_3:+843479 Msa0269810:CDS
TCCACACACTTTCCATGCGC+CGG 0.510157 2_3:-843593 None:intergenic
AAGAATGGAGTGGTGGAAGC+TGG 0.512376 2_3:+843355 Msa0269810:CDS
GAACCAAGGGTGATAGTGTC+CGG 0.513905 2_3:+843574 Msa0269810:CDS
AAGAGATCGGGTATGGGGAG+GGG 0.523912 2_3:+843486 Msa0269810:CDS
AGAAAAGTGTGAGGCTTGGA+GGG 0.526565 2_3:+843548 Msa0269810:CDS
TCAGGTTTCATCATAACCAT+AGG 0.529456 2_3:+843049 Msa0269810:CDS
CTCAACACTACTCTTCCACT+TGG 0.532729 2_3:+843283 Msa0269810:CDS
GGGTGATAGTGTCCGGCGCA+TGG 0.534538 2_3:+843581 Msa0269810:CDS
TACGAAAAGAGATCGGGTAT+GGG 0.536489 2_3:+843480 Msa0269810:CDS
ACAGCATGAACAACAGCTGT+TGG 0.541536 2_3:-843026 None:intergenic
GGAAAGAAAAGTGTGAGGCT+TGG 0.543228 2_3:+843544 Msa0269810:CDS
TTGGTATACGAAAAGAGATC+GGG 0.544628 2_3:+843474 Msa0269810:CDS
AGCTAAGAAGAATGGAGTGG+TGG 0.547361 2_3:+843348 Msa0269810:CDS
AAAAGAGATCGGGTATGGGG+AGG 0.556071 2_3:+843484 Msa0269810:CDS
GCGCCGGACACTATCACCCT+TGG 0.558967 2_3:-843577 None:intergenic
CTGTAAATGGATTCTATCAA+GGG 0.559809 2_3:+843314 Msa0269810:CDS
TTTGGTATACGAAAAGAGAT+CGG 0.560749 2_3:+843473 Msa0269810:CDS
TGGTAAAGTTTATCTAAGGG+TGG 0.560905 2_3:+843417 Msa0269810:CDS
TGATGGTAAAGTTTATCTAA+GGG 0.567329 2_3:+843414 Msa0269810:CDS
ACGAAAAGAGATCGGGTATG+GGG 0.597221 2_3:+843481 Msa0269810:CDS
GGCTTGGAGGGAATGAACCA+AGG 0.634018 2_3:+843560 Msa0269810:CDS
GCTTGGAGGGAATGAACCAA+GGG 0.643282 2_3:+843561 Msa0269810:CDS
TGTGGAAATTGGAGATTCTG+GGG 0.648415 2_3:+843513 Msa0269810:CDS
GGCGCATGGAAAGTGTGTGG+AGG 0.656040 2_3:+843595 Msa0269810:CDS
AAAAGCTAAGAAGAATGGAG+TGG 0.661701 2_3:+843345 Msa0269810:CDS
TCCGGCGCATGGAAAGTGTG+TGG 0.671773 2_3:+843592 Msa0269810:CDS
TTTACAGTAGCATTACCAAG+TGG 0.674013 2_3:-843298 None:intergenic
AAATGGGAAAGAAAAGTGTG+AGG 0.693699 2_3:+843539 Msa0269810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATACCTTTATCTTTATTA+GGG - chr2_3:843222-843241 None:intergenic 20.0%
!! TGAATACCTTTATCTTTATT+AGG - chr2_3:843223-843242 None:intergenic 20.0%
!!! TTAAGTATAAGATTTTGGTT+TGG + chr2_3:843455-843474 Msa0269810:CDS 20.0%
!! ATTTTGTCTATGTAAAGCTT+TGG - chr2_3:843118-843137 None:intergenic 25.0%
! GTTAACCCTAATAAAGATAA+AGG + chr2_3:843214-843233 Msa0269810:CDS 25.0%
!! TTGATGGTAAAGTTTATCTA+AGG + chr2_3:843413-843432 Msa0269810:CDS 25.0%
! TGATGGTAAAGTTTATCTAA+GGG + chr2_3:843414-843433 Msa0269810:CDS 25.0%
TATGCTTAGCCATATGAATA+GGG - chr2_3:843083-843102 None:intergenic 30.0%
!! GTTAACAAGTTTTAACGTGA+AGG - chr2_3:843200-843219 None:intergenic 30.0%
AGGTGAAGTTAAGATGAATA+GGG - chr2_3:843252-843271 None:intergenic 30.0%
AAGGTGAAGTTAAGATGAAT+AGG - chr2_3:843253-843272 None:intergenic 30.0%
!! AGTGTTGAGAGTATTGTAAA+AGG - chr2_3:843272-843291 None:intergenic 30.0%
! ACTGTAAATGGATTCTATCA+AGG + chr2_3:843313-843332 Msa0269810:CDS 30.0%
! CTGTAAATGGATTCTATCAA+GGG + chr2_3:843314-843333 Msa0269810:CDS 30.0%
GAGAAAAAAGCTAAGAAGAA+TGG + chr2_3:843340-843359 Msa0269810:CDS 30.0%
AATTTAACTGCGAGTGTTAA+GGG + chr2_3:843385-843404 Msa0269810:CDS 30.0%
! TGCGGTTAAGTATAAGATTT+TGG + chr2_3:843450-843469 Msa0269810:CDS 30.0%
TTTGGTATACGAAAAGAGAT+CGG + chr2_3:843473-843492 Msa0269810:CDS 30.0%
!!! TTTTGGGTCTTGTTTATCAT+TGG + chr2_3:843637-843656 Msa0269810:CDS 30.0%
TCAGGTTTCATCATAACCAT+AGG + chr2_3:843049-843068 Msa0269810:CDS 35.0%
CATCATAACCATAGGCTTAA+TGG + chr2_3:843057-843076 Msa0269810:CDS 35.0%
GTATGCTTAGCCATATGAAT+AGG - chr2_3:843084-843103 None:intergenic 35.0%
!!! TGTGTTTAGGGTTTTGTTGA+GGG - chr2_3:843152-843171 None:intergenic 35.0%
!!! TTGTGTTTAGGGTTTTGTTG+AGG - chr2_3:843153-843172 None:intergenic 35.0%
!! TGATCTTGCTGTTGTGTTTA+GGG - chr2_3:843164-843183 None:intergenic 35.0%
!! TTGATCTTGCTGTTGTGTTT+AGG - chr2_3:843165-843184 None:intergenic 35.0%
! TTTTAACGTGAAGGTGATAG+TGG - chr2_3:843191-843210 None:intergenic 35.0%
TTTACAGTAGCATTACCAAG+TGG - chr2_3:843301-843320 None:intergenic 35.0%
!! CTTGGTAATGCTACTGTAAA+TGG + chr2_3:843301-843320 Msa0269810:CDS 35.0%
AAAAGCTAAGAAGAATGGAG+TGG + chr2_3:843345-843364 Msa0269810:CDS 35.0%
GAATTTAACTGCGAGTGTTA+AGG + chr2_3:843384-843403 Msa0269810:CDS 35.0%
TGGTAAAGTTTATCTAAGGG+TGG + chr2_3:843417-843436 Msa0269810:CDS 35.0%
!!! AAGGGTGGATTATTTTACTG+CGG + chr2_3:843432-843451 Msa0269810:CDS 35.0%
TTGGTATACGAAAAGAGATC+GGG + chr2_3:843474-843493 Msa0269810:CDS 35.0%
AATGTGGAAATTGGAGATTC+TGG + chr2_3:843511-843530 Msa0269810:CDS 35.0%
ATGTGGAAATTGGAGATTCT+GGG + chr2_3:843512-843531 Msa0269810:CDS 35.0%
! AAATGGGAAAGAAAAGTGTG+AGG + chr2_3:843539-843558 Msa0269810:CDS 35.0%
!! GTCTTGTTTATCATTGGTCT+TGG + chr2_3:843643-843662 Msa0269810:CDS 35.0%
GCTTAATGGCCCTATTCATA+TGG + chr2_3:843071-843090 Msa0269810:CDS 40.0%
!! AGTGTTAAGGGTGTTGTTGA+TGG + chr2_3:843397-843416 Msa0269810:CDS 40.0%
ATACGAAAAGAGATCGGGTA+TGG + chr2_3:843479-843498 Msa0269810:CDS 40.0%
TACGAAAAGAGATCGGGTAT+GGG + chr2_3:843480-843499 Msa0269810:CDS 40.0%
TGTGGAAATTGGAGATTCTG+GGG + chr2_3:843513-843532 Msa0269810:CDS 40.0%
CAGCATGAACAACAGCTGTT+GGG - chr2_3:843028-843047 None:intergenic 45.0%
ACAGCATGAACAACAGCTGT+TGG - chr2_3:843029-843048 None:intergenic 45.0%
AGCTGTTGTTCATGCTGTTC+AGG + chr2_3:843031-843050 Msa0269810:CDS 45.0%
GAATAGGGCCATTAAGCCTA+TGG - chr2_3:843068-843087 None:intergenic 45.0%
!!! TTTAGGGTTTTGTTGAGGGC+AGG - chr2_3:843148-843167 None:intergenic 45.0%
CTCAACACTACTCTTCCACT+TGG + chr2_3:843283-843302 Msa0269810:CDS 45.0%
AGCTAAGAAGAATGGAGTGG+TGG + chr2_3:843348-843367 Msa0269810:CDS 45.0%
ACGAAAAGAGATCGGGTATG+GGG + chr2_3:843481-843500 Msa0269810:CDS 45.0%
! TGGAGATTCTGGGGAGAAAA+TGG + chr2_3:843522-843541 Msa0269810:CDS 45.0%
! GGAGATTCTGGGGAGAAAAT+GGG + chr2_3:843523-843542 Msa0269810:CDS 45.0%
! GGAAAGAAAAGTGTGAGGCT+TGG + chr2_3:843544-843563 Msa0269810:CDS 45.0%
! AAGAAAAGTGTGAGGCTTGG+AGG + chr2_3:843547-843566 Msa0269810:CDS 45.0%
! AGAAAAGTGTGAGGCTTGGA+GGG + chr2_3:843548-843567 Msa0269810:CDS 45.0%
! ATCGTGCGCTTCTTGTGTTT+TGG + chr2_3:843620-843639 Msa0269810:CDS 45.0%
! TCGTGCGCTTCTTGTGTTTT+GGG + chr2_3:843621-843640 Msa0269810:CDS 45.0%
! AAGAATGGAGTGGTGGAAGC+TGG + chr2_3:843355-843374 Msa0269810:CDS 50.0%
AAAAGAGATCGGGTATGGGG+AGG + chr2_3:843484-843503 Msa0269810:CDS 50.0%
AAAGAGATCGGGTATGGGGA+GGG + chr2_3:843485-843504 Msa0269810:CDS 50.0%
GGAGGGGCAAATGTGGAAAT+TGG + chr2_3:843502-843521 Msa0269810:CDS 50.0%
GCTTGGAGGGAATGAACCAA+GGG + chr2_3:843561-843580 Msa0269810:CDS 50.0%
! GAACCAAGGGTGATAGTGTC+CGG + chr2_3:843574-843593 Msa0269810:CDS 50.0%
!!! GGGTTTTGTTGAGGGCAGGA+AGG - chr2_3:843144-843163 None:intergenic 55.0%
AAGAGATCGGGTATGGGGAG+GGG + chr2_3:843486-843505 Msa0269810:CDS 55.0%
GGCTTGGAGGGAATGAACCA+AGG + chr2_3:843560-843579 Msa0269810:CDS 55.0%
TCCACACACTTTCCATGCGC+CGG - chr2_3:843596-843615 None:intergenic 55.0%
GGTATGGGGAGGGGCAAATG+TGG + chr2_3:843495-843514 Msa0269810:CDS 60.0%
! TCCGGCGCATGGAAAGTGTG+TGG + chr2_3:843592-843611 Msa0269810:CDS 60.0%
! GGCGCATGGAAAGTGTGTGG+AGG + chr2_3:843595-843614 Msa0269810:CDS 60.0%
GCGCCGGACACTATCACCCT+TGG - chr2_3:843580-843599 None:intergenic 65.0%
! GGGTGATAGTGTCCGGCGCA+TGG + chr2_3:843581-843600 Msa0269810:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 843010 843684 843010 ID=Msa0269810;Name=Msa0269810
chr2_3 mRNA 843010 843684 843010 ID=Msa0269810-mRNA-1;Parent=Msa0269810;Name=Msa0269810-mRNA-1;_AED=0.01;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|224
chr2_3 exon 843010 843684 843010 ID=Msa0269810-mRNA-1:exon:397;Parent=Msa0269810-mRNA-1
chr2_3 CDS 843010 843684 843010 ID=Msa0269810-mRNA-1:cds;Parent=Msa0269810-mRNA-1
Gene Sequence

>Msa0269810

ATGGCATCATCAAAACCCAACAGCTGTTGTTCATGCTGTTCAGGTTTCATCATAACCATAGGCTTAATGGCCCTATTCATATGGCTAAGCATACGTGTCGATGAACCAAAGCTTTACATAGACAAAATTTACCTTCCTGCCCTCAACAAAACCCTAAACACAACAGCAAGATCAAACACCACTATCACCTTCACGTTAAAACTTGTTAACCCTAATAAAGATAAAGGTATTCAATATGACCCTATTCATCTTAACTTCACCTTTTACAATACTCTCAACACTACTCTTCCACTTGGTAATGCTACTGTAAATGGATTCTATCAAGGGCATGAGAAAAAAGCTAAGAAGAATGGAGTGGTGGAAGCTGGTGTTAAGAATTTAACTGCGAGTGTTAAGGGTGTTGTTGATGGTAAAGTTTATCTAAGGGTGGATTATTTTACTGCGGTTAAGTATAAGATTTTGGTTTGGTATACGAAAAGAGATCGGGTATGGGGAGGGGCAAATGTGGAAATTGGAGATTCTGGGGAGAAAATGGGAAAGAAAAGTGTGAGGCTTGGAGGGAATGAACCAAGGGTGATAGTGTCCGGCGCATGGAAAGTGTGTGGAGGCTATCGTGCGCTTCTTGTGTTTTGGGTCTTGTTTATCATTGGTCTTGGTTTACTTGACTTTGACTGA

Protein sequence

>Msa0269810

MASSKPNSCCSCCSGFIITIGLMALFIWLSIRVDEPKLYIDKIYLPALNKTLNTTARSNTTITFTLKLVNPNKDKGIQYDPIHLNFTFYNTLNTTLPLGNATVNGFYQGHEKKAKKNGVVEAGVKNLTASVKGVVDGKVYLRVDYFTAVKYKILVWYTKRDRVWGGANVEIGDSGEKMGKKSVRLGGNEPRVIVSGAWKVCGGYRALLVFWVLFIIGLGLLDFD*