AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0567440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0567440 MtrunA17_Chr8g0382201 95.052 384 15 3 1 384 86 465 0.0 700
Msa0567440 MtrunA17_Chr3g0115801 62.903 372 119 5 17 384 30 386 5.33e-147 426
Msa0567440 MtrunA17_Chr1g0149111 35.211 284 160 4 112 391 137 400 1.65e-46 166
Msa0567440 MtrunA17_Chr1g0149111 30.108 186 107 4 200 384 132 295 1.04e-15 79.0
Msa0567440 MtrunA17_Chr1g0149111 27.072 181 128 2 108 284 227 407 2.37e-15 77.8
Msa0567440 MtrunA17_Chr3g0137941 34.507 284 162 4 112 391 120 383 4.51e-46 164
Msa0567440 MtrunA17_Chr3g0137941 26.776 183 130 2 114 292 216 398 2.70e-15 77.4
Msa0567440 MtrunA17_Chr4g0063561 34.859 284 161 5 112 391 121 384 3.93e-42 154
Msa0567440 MtrunA17_Chr4g0063561 29.508 183 107 4 203 384 119 280 7.67e-14 73.2
Msa0567440 MtrunA17_Chr4g0063561 27.326 172 122 2 116 284 220 391 2.43e-13 71.6
Msa0567440 MtrunA17_Chr7g0242311 33.456 272 139 10 125 384 98 339 7.91e-33 128
Msa0567440 MtrunA17_Chr7g0242311 28.358 201 137 3 105 305 178 371 9.07e-17 82.0
Msa0567440 MtrunA17_Chr4g0006851 33.333 270 138 11 125 382 100 339 1.38e-31 124
Msa0567440 MtrunA17_Chr4g0006851 27.461 193 133 3 113 305 188 373 1.32e-15 78.6
Msa0567440 MtrunA17_Chr7g0240341 27.759 299 174 6 108 384 19 297 1.49e-31 123
Msa0567440 MtrunA17_Chr7g0240341 23.529 187 122 5 113 285 133 312 4.75e-11 63.9
Msa0567440 MtrunA17_Chr5g0396801 28.655 342 190 8 86 391 5 328 1.15e-29 119
Msa0567440 MtrunA17_Chr5g0396801 26.396 197 121 4 113 285 140 336 1.62e-17 83.6
Msa0567440 MtrunA17_Chr7g0252401 30.479 292 172 10 108 391 201 469 1.96e-29 120
Msa0567440 MtrunA17_Chr7g0252401 27.528 178 122 3 114 286 303 478 2.44e-14 75.1
Msa0567440 MtrunA17_Chr8g0385571 29.730 296 178 10 105 391 218 492 1.41e-28 118
Msa0567440 MtrunA17_Chr8g0385571 27.222 180 123 3 113 284 320 499 2.78e-14 75.1
Msa0567440 MtrunA17_Chr3g0131501 29.355 310 183 11 92 391 187 470 8.85e-28 115
Msa0567440 MtrunA17_Chr3g0131501 21.284 296 201 8 6 284 197 477 3.96e-11 65.1
Msa0567440 MtrunA17_Chr8g0347451 28.115 313 180 7 109 391 46 343 1.43e-26 110
Msa0567440 MtrunA17_Chr8g0347451 26.131 199 121 4 113 285 153 351 4.86e-18 85.5
Msa0567440 MtrunA17_Chr2g0296061 25.249 301 179 7 116 382 17 305 2.30e-26 109
Msa0567440 MtrunA17_Chr2g0296061 26.442 208 127 5 108 290 120 326 4.79e-15 76.3
Msa0567440 MtrunA17_Chr4g0067231 25.902 305 174 8 116 382 16 306 4.34e-26 108
Msa0567440 MtrunA17_Chr4g0067231 26.768 198 121 4 116 290 131 327 1.24e-16 80.9
Msa0567440 MtrunA17_Chr8g0393031 30.986 284 152 10 120 381 24 285 2.52e-25 105
Msa0567440 MtrunA17_Chr6g0488081 26.821 302 177 7 112 391 17 296 3.70e-25 105
Msa0567440 MtrunA17_Chr3g0135861 26.603 312 172 7 112 391 33 319 6.41e-25 105
Msa0567440 MtrunA17_Chr5g0436041 26.599 297 178 6 108 384 28 304 9.28e-24 101
Msa0567440 MtrunA17_Chr8g0386871 24.556 338 216 8 70 392 265 578 2.00e-22 100
Msa0567440 MtrunA17_Chr8g0386871 28.652 178 122 3 113 287 412 587 3.73e-17 84.3
Msa0567440 MtrunA17_Chr4g0000261 24.138 290 178 6 116 384 68 336 1.19e-21 96.3
Msa0567440 MtrunA17_Chr4g0000261 29.949 197 114 5 205 391 64 246 4.00e-13 70.5
Msa0567440 MtrunA17_Chr3g0135801 25.387 323 195 10 83 382 4 303 2.25e-19 89.0
Msa0567440 MtrunA17_Chr8g0372531 25.098 255 172 8 145 391 52 295 7.50e-15 75.5
Msa0567440 MtrunA17_Chr7g0269701 26.689 296 174 12 116 391 24 296 2.44e-14 73.9
Msa0567440 MtrunA17_Chr8g0383321 23.171 328 208 9 73 392 5 296 1.01e-13 72.0
Msa0567440 MtrunA17_Chr8g0383321 27.168 173 123 3 113 284 132 302 1.27e-12 68.9
Msa0567440 MtrunA17_Chr6g0488341 22.594 239 151 4 151 384 5 214 8.75e-13 68.2
Msa0567440 MtrunA17_Chr3g0088301 23.549 293 176 10 117 385 96 364 2.14e-12 68.6
Msa0567440 MtrunA17_Chr1g0201361 25.275 182 131 4 113 291 131 310 2.19e-12 67.8
Msa0567440 MtrunA17_Chr1g0201361 25.106 235 149 6 160 392 86 295 9.75e-11 62.8
Msa0567440 MtrunA17_Chr4g0040341 24.573 293 173 12 117 385 78 346 3.70e-12 67.8
Msa0567440 MtrunA17_Chr1g0200171 27.907 172 118 3 126 293 621 790 7.23e-12 67.8
Msa0567440 MtrunA17_Chr2g0328891 23.929 280 165 10 130 385 82 337 9.15e-12 66.6
Msa0567440 MtrunA17_Chr5g0431531 27.869 183 120 5 113 285 132 312 2.74e-11 64.7
Msa0567440 MtrunA17_Chr8g0352031 23.129 294 177 9 117 385 71 340 5.92e-11 63.9
Msa0567440 MtrunA17_Chr6g0463351 24.479 192 133 4 116 298 162 350 8.99e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0567440 AT5G64970.1 64.677 402 117 5 9 391 18 413 1.20e-167 479
Msa0567440 AT5G64970.3 65.394 393 112 4 9 383 18 404 1.63e-167 478
Msa0567440 AT5G64970.2 79.259 270 56 0 115 384 12 281 2.14e-153 438
Msa0567440 AT1G78180.1 57.692 390 150 4 10 391 24 406 1.39e-135 397
Msa0567440 AT4G32400.1 33.803 284 164 5 112 391 112 375 3.13e-43 157
Msa0567440 AT4G32400.1 29.508 183 106 5 203 384 110 270 2.56e-14 75.1
Msa0567440 AT4G32400.1 26.519 181 117 4 114 284 208 382 7.85e-13 70.5
Msa0567440 AT3G55640.1 29.781 319 173 8 86 384 21 308 2.09e-33 129
Msa0567440 AT3G55640.1 21.739 184 135 4 109 284 140 322 7.51e-11 63.9
Msa0567440 AT3G55640.4 29.781 319 173 8 86 384 21 308 2.09e-33 129
Msa0567440 AT3G55640.4 21.739 184 135 4 109 284 140 322 7.51e-11 63.9
Msa0567440 AT2G37890.1 27.961 304 179 8 101 384 32 315 8.13e-32 125
Msa0567440 AT2G37890.1 26.768 198 134 5 104 292 142 337 4.75e-14 73.6
Msa0567440 AT3G53940.1 29.000 300 171 7 106 384 65 343 2.48e-31 124
Msa0567440 AT3G53940.1 25.926 189 131 4 104 284 170 357 1.36e-14 75.5
Msa0567440 AT3G51870.1 30.627 271 148 10 125 384 102 343 1.34e-29 119
Msa0567440 AT3G51870.1 24.865 185 135 3 113 297 190 370 2.45e-13 72.0
Msa0567440 AT4G01100.1 28.615 325 187 8 97 391 25 334 1.76e-29 119
Msa0567440 AT4G01100.1 25.628 199 122 4 113 285 144 342 1.78e-15 78.2
Msa0567440 AT5G51050.1 28.425 292 184 7 105 391 214 485 3.00e-29 120
Msa0567440 AT5G51050.1 26.016 246 168 6 51 286 253 494 3.14e-16 81.6
Msa0567440 AT5G01500.1 32.090 268 144 10 125 382 130 369 3.22e-29 119
Msa0567440 AT5G01500.1 27.083 192 136 2 106 297 211 398 2.48e-13 72.0
Msa0567440 AT3G55640.2 33.180 217 119 5 86 287 21 226 1.87e-27 110
Msa0567440 AT3G55640.3 33.180 217 119 5 86 287 21 226 1.87e-27 110
Msa0567440 AT4G01100.2 27.711 332 181 8 97 384 25 341 7.11e-27 111
Msa0567440 AT4G01100.2 25.758 198 121 4 113 284 158 355 1.99e-15 78.2
Msa0567440 AT4G01100.3 29.452 292 161 8 123 384 1 277 7.11e-27 110
Msa0567440 AT4G01100.3 25.628 199 122 4 113 285 94 292 1.15e-15 78.2
Msa0567440 AT4G26180.1 26.974 304 175 8 112 392 19 298 1.43e-26 110
Msa0567440 AT4G26180.2 26.780 295 171 7 112 383 19 291 2.52e-26 108
Msa0567440 AT3G53940.2 32.178 202 121 4 106 291 65 266 4.24e-25 105
Msa0567440 AT3G20240.1 26.871 294 165 10 116 384 54 322 3.02e-23 100
Msa0567440 AT3G20240.1 26.404 178 128 2 116 290 165 342 4.24e-11 64.7
Msa0567440 AT3G20240.1 25.521 192 139 3 200 391 45 232 7.83e-11 63.9
Msa0567440 AT5G07320.1 27.206 272 172 5 123 391 218 466 2.10e-22 100
Msa0567440 AT5G07320.1 25.134 187 131 5 105 285 291 474 2.58e-13 72.4
Msa0567440 AT5G61810.2 26.736 288 189 7 105 391 56 322 7.59e-22 96.7
Msa0567440 AT5G61810.1 25.694 288 192 5 105 391 199 465 1.33e-21 97.8
Msa0567440 AT5G48970.1 24.585 301 184 8 116 382 24 315 5.12e-21 94.4
Msa0567440 AT5G48970.1 24.576 236 143 6 89 290 102 336 7.23e-14 73.2
Msa0567440 AT5G48970.1 23.372 261 157 7 209 448 24 262 1.43e-11 66.2
Msa0567440 AT1G14560.2 29.924 264 132 9 110 348 23 258 5.00e-20 90.9
Msa0567440 AT1G14560.1 29.139 302 150 12 125 392 38 309 6.41e-20 90.9
Msa0567440 AT4G11440.1 25.784 287 187 6 112 392 328 594 3.43e-19 90.9
Msa0567440 AT4G11440.2 25.784 287 187 6 112 392 328 594 3.43e-19 90.9
Msa0567440 AT3G21390.2 24.832 298 186 7 116 382 43 333 6.72e-19 88.6
Msa0567440 AT3G21390.2 26.804 194 115 4 116 282 154 347 3.65e-14 74.3
Msa0567440 AT3G21390.2 25.333 225 132 6 188 392 16 224 3.23e-12 68.2
Msa0567440 AT3G21390.1 23.826 298 189 7 116 382 21 311 8.97e-19 87.8
Msa0567440 AT3G21390.1 26.804 194 115 4 116 282 132 325 4.26e-14 73.9
Msa0567440 AT3G21390.1 24.771 218 128 6 195 392 1 202 9.86e-11 63.5
Msa0567440 AT5G48970.2 24.901 253 164 6 147 382 1 244 1.53e-18 85.9
Msa0567440 AT5G48970.2 23.729 236 145 5 89 290 31 265 3.53e-14 73.2
Msa0567440 AT5G56450.1 25.000 300 178 8 109 382 29 307 2.02e-17 83.6
Msa0567440 AT5G17400.1 29.137 278 145 10 130 381 30 281 3.48e-17 82.4
Msa0567440 AT1G79900.1 26.816 179 124 4 117 291 113 288 7.00e-15 75.5
Msa0567440 AT5G66380.1 25.097 259 166 7 145 391 48 290 2.37e-14 74.3
Msa0567440 AT5G26200.1 26.736 288 162 9 126 383 51 319 3.40e-14 74.3
Msa0567440 AT1G34065.2 27.746 173 122 3 113 284 155 325 4.66e-14 73.6
Msa0567440 AT1G34065.2 24.028 283 177 9 112 392 73 319 1.46e-12 69.3
Msa0567440 AT4G28390.1 24.573 293 173 10 117 385 86 354 5.02e-14 73.9
Msa0567440 AT4G28390.2 24.573 293 173 10 117 385 86 354 5.02e-14 73.9
Msa0567440 AT1G34065.1 27.746 173 122 3 113 284 162 332 5.31e-14 73.6
Msa0567440 AT1G34065.1 24.028 283 177 9 112 392 80 326 1.38e-12 69.3
Msa0567440 AT4G39460.2 26.523 279 167 8 116 392 60 302 2.66e-13 71.2
Msa0567440 AT4G39460.2 25.126 199 133 5 113 306 138 325 1.70e-12 68.9
Msa0567440 AT4G39460.1 26.523 279 167 8 116 392 60 302 2.66e-13 71.2
Msa0567440 AT4G39460.1 25.126 199 133 5 113 306 138 325 1.70e-12 68.9
Msa0567440 AT4G39460.3 26.523 279 167 8 116 392 60 302 2.66e-13 71.2
Msa0567440 AT4G39460.3 25.126 199 133 5 113 306 138 325 1.70e-12 68.9
Msa0567440 AT5G46800.2 28.175 252 142 10 152 387 55 283 3.90e-13 70.5
Msa0567440 AT5G46800.3 28.175 252 142 10 152 387 55 283 3.90e-13 70.5
Msa0567440 AT5G46800.1 28.175 252 142 10 152 387 55 283 3.90e-13 70.5
Msa0567440 AT2G26360.1 31.677 161 103 5 133 288 219 377 1.52e-12 69.3
Msa0567440 AT5G09470.1 26.000 250 156 7 36 271 82 316 1.58e-12 68.9
Msa0567440 AT3G08580.2 23.718 312 188 10 100 385 69 356 4.21e-12 68.2
Msa0567440 AT3G08580.1 23.718 312 188 10 100 385 69 356 4.21e-12 68.2
Msa0567440 AT5G13490.1 23.077 312 190 11 100 385 73 360 1.05e-11 67.0
Msa0567440 AT5G13490.2 23.077 312 190 11 100 385 73 360 1.05e-11 67.0
Msa0567440 AT2G30160.1 24.638 207 141 5 93 287 118 321 2.38e-11 65.5
Msa0567440 AT4G27940.1 26.136 264 151 9 143 382 145 388 5.00e-11 65.1
Msa0567440 AT1G07030.1 25.532 188 131 3 109 287 129 316 5.65e-11 64.3

Find 141 sgRNAs with CRISPR-Local

Find 283 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAAGGTTTGAAAGGATTT+TGG 0.117339 4_1:+68785081 Msa0567440:CDS
TCTTCGAACGGCACCCTTTA+AGG 0.134029 4_1:+68785124 Msa0567440:CDS
ATGTCTCGTGTTTATTTAAT+TGG 0.207547 4_1:-68783749 None:intergenic
ACCATTAGAACTTTATTATA+TGG 0.235225 4_1:+68786187 Msa0567440:CDS
GATGATTCCTTCTTCTTTCC+AGG 0.251005 4_1:+68783951 Msa0567440:CDS
GACCCTCTTCTTTCATATAT+TGG 0.263621 4_1:-68786129 None:intergenic
TGTTCCAACTTTGATAAATT+AGG 0.282644 4_1:-68786518 None:intergenic
TTCAAAGAAGGAGAATATTC+AGG 0.285885 4_1:+68784205 Msa0567440:CDS
TCTGTTCCAACGTTGTATTT+TGG 0.311110 4_1:+68786494 Msa0567440:CDS
TGAGTAATCGGCCACCTCTT+AGG 0.320752 4_1:-68787209 None:intergenic
CTCCTTCTTTGAAACTTGAT+TGG 0.323254 4_1:-68784195 None:intergenic
TGCTTTGGAACAACTGGAAT+TGG 0.323427 4_1:+68786162 Msa0567440:CDS
TTTGTTGCTGGTGCTGCTGC+AGG 0.327887 4_1:+68785230 Msa0567440:CDS
TTATTATATGGTGCTATTGC+GGG 0.329863 4_1:+68786199 Msa0567440:CDS
AAGGAGACAGCTTCAATTGC+AGG 0.333519 4_1:+68786261 Msa0567440:CDS
TTGAAACAACAGATATAGTT+TGG 0.344457 4_1:+68787142 Msa0567440:three_prime_UTR
AATAAACTAGTCAGATTATC+GGG 0.348422 4_1:+68785179 Msa0567440:CDS
ATTAACTCCCAGCTTATTGC+AGG 0.354263 4_1:+68786363 Msa0567440:CDS
CATATCCCTTTGAAGTTGTA+AGG 0.358768 4_1:+68786242 Msa0567440:CDS
ACAGGAAACAGTGGTAGAAT+TGG 0.363567 4_1:-68784042 None:intergenic
TGGACCTAATTTATCAAAGT+TGG 0.364548 4_1:+68786514 Msa0567440:CDS
GACAGCTTCAATTGCAGGTT+AGG 0.374549 4_1:+68786266 Msa0567440:CDS
GAAGATGTCGTCCAATGATA+TGG 0.376812 4_1:-68787450 None:intergenic
TTATAAAATGTGTGAGTAAT+CGG 0.377865 4_1:-68787221 None:intergenic
CACACGTTGTTGTTGTGGTT+TGG 0.378003 4_1:-68783849 None:intergenic
GCTGAATGCTTTGGAACAAC+TGG 0.379168 4_1:+68786156 Msa0567440:CDS
AAGGAGAATATTCAGGTTCA+TGG 0.388663 4_1:+68784212 Msa0567440:CDS
CTGGCAATGCTTTGATGAAT+AGG 0.390978 4_1:+68787288 Msa0567440:three_prime_UTR
CGCCTCTTCATTGTGCTGTA+AGG 0.391075 4_1:-68783706 None:intergenic
AGTCAACACTTGGAATTTCA+TGG 0.398124 4_1:-68786678 None:intergenic
TCCTTGAGTTTGTCTATAAA+CGG 0.400844 4_1:+68784140 Msa0567440:CDS
TTTCTTGGAATATCAGGAAA+AGG 0.403079 4_1:+68784066 Msa0567440:CDS
AGCACCTGGCGGTGAAGCAT+TGG 0.405799 4_1:+68785934 Msa0567440:CDS
AACAAATCTCTCAAAATTTG+TGG 0.410653 4_1:-68785213 None:intergenic
ACCATATAATAAAGTTCTAA+TGG 0.412646 4_1:-68786188 None:intergenic
TGAAGGTGCCATGCTGATAA+TGG 0.413657 4_1:-68786032 None:intergenic
AAATAAACTAGTCAGATTAT+CGG 0.415963 4_1:+68785178 Msa0567440:CDS
CATTGTGCTGTAAGGTCTGT+TGG 0.432168 4_1:-68783698 None:intergenic
TTTGAGATTCTTAAGGTTCA+AGG 0.432482 4_1:+68784095 Msa0567440:CDS
AGGTTATACCTGCAATAAGC+TGG 0.438087 4_1:-68786371 None:intergenic
ATACACTGTCTAGGTCTCAT+TGG 0.449140 4_1:+68787256 Msa0567440:three_prime_UTR
GGGTCAAGAGCTGAATGCTT+TGG 0.450331 4_1:+68786147 Msa0567440:CDS
TTGTTGCTGGTGCTGCTGCA+GGG 0.450597 4_1:+68785231 Msa0567440:CDS
CGCATTTCATTCCTTTCGAT+CGG 0.454099 4_1:+68783802 Msa0567440:five_prime_UTR
GCTTTGGAACAACTGGAATT+GGG 0.455395 4_1:+68786163 Msa0567440:CDS
CTCTGTTATCTTGCAAGAAA+AGG 0.456382 4_1:-68786722 None:intergenic
ATTCTTAAGGTTCAAGGTGC+TGG 0.456442 4_1:+68784101 Msa0567440:CDS
GCACCTGGCGGTGAAGCATT+GGG 0.459088 4_1:+68785935 Msa0567440:CDS
GGTGTTCCTGCTCTCTATGC+AGG 0.463872 4_1:+68786340 Msa0567440:CDS
GAAGCATTGGGAGGTGTAAT+CGG 0.464975 4_1:+68785947 Msa0567440:CDS
ATTATCAGCATGGCACCTTC+AGG 0.465225 4_1:+68786034 Msa0567440:CDS
CCTCCCAATGCTTCACCGCC+AGG 0.467880 4_1:-68785938 None:intergenic
GTCTCATTGGATTCTACTGC+TGG 0.468406 4_1:+68787269 Msa0567440:three_prime_UTR
GTAGAATTGGATGAAGAAGA+TGG 0.469567 4_1:-68784029 None:intergenic
ATGACCAAACACCTCTGGTC+TGG 0.470639 4_1:+68784251 Msa0567440:CDS
TGTCGTCCAATGATATGGTT+AGG 0.470864 4_1:-68787445 None:intergenic
AGCACCAGACCAGAGGTGTT+TGG 0.476230 4_1:-68784255 None:intergenic
AAGGTTCAAGGTGCTGGTGC+TGG 0.476407 4_1:+68784107 Msa0567440:CDS
GAGAATATTCTCATCTTCAA+TGG 0.481124 4_1:-68787165 None:intergenic
AACCTTGCTCTGCTTGCCTA+TGG 0.481521 4_1:+68785262 Msa0567440:CDS
TTGGCTTATTAAGAAGAAGA+AGG 0.484107 4_1:-68783830 None:intergenic
CAGACAATTGCAACTTCTCA+AGG 0.486083 4_1:+68785065 Msa0567440:CDS
TGTCTCCTTACAACTTCAAA+GGG 0.489648 4_1:-68786247 None:intergenic
GCAACTTCTCAAGGTTTGAA+AGG 0.494025 4_1:+68785074 Msa0567440:CDS
TTTATTATATGGTGCTATTG+CGG 0.495204 4_1:+68786198 Msa0567440:CDS
AATATTCAGGTTCATGGATC+GGG 0.498629 4_1:+68784218 Msa0567440:CDS
TTCTTGGAATATCAGGAAAA+GGG 0.503984 4_1:+68784067 Msa0567440:CDS
ATCCAATCAAGTTTCAAAGA+AGG 0.504802 4_1:+68784193 Msa0567440:CDS
CTTTCAGATCAGGACAGTAA+TGG 0.504931 4_1:+68785910 Msa0567440:intron
CGAGGTACGAGAAGTTGATC+TGG 0.505349 4_1:-68783879 None:intergenic
AGAGATAATTTGTTAGCAGT+AGG 0.506311 4_1:+68787386 Msa0567440:three_prime_UTR
AGAAGGACGAGCCGATCGAA+AGG 0.510509 4_1:-68783813 None:intergenic
TGGGATGCTGCAGGTAATGA+AGG 0.516343 4_1:-68784009 None:intergenic
CTGTCTCCTTACAACTTCAA+AGG 0.518936 4_1:-68786248 None:intergenic
TAGTGTCCTAACCATATCAT+TGG 0.522874 4_1:+68787439 Msa0567440:three_prime_UTR
ATATCATTGGACGACATCTT+CGG 0.524723 4_1:+68787452 Msa0567440:three_prime_UTR
ACAGCTTCAATTGCAGGTTA+GGG 0.529291 4_1:+68786267 Msa0567440:CDS
TGTTGCTGGTGCTGCTGCAG+GGG 0.530011 4_1:+68785232 Msa0567440:CDS
TCAACATGACCAAACACCTC+TGG 0.535107 4_1:+68784246 Msa0567440:CDS
AAGACCACCTGGAAAGAAGA+AGG 0.539434 4_1:-68783958 None:intergenic
GTCAACACTTGGAATTTCAT+GGG 0.544807 4_1:-68786677 None:intergenic
GAATATTCAGGTTCATGGAT+CGG 0.545360 4_1:+68784217 Msa0567440:CDS
TTGCATAGATAGTCAACACT+TGG 0.546500 4_1:-68786688 None:intergenic
CCTTCAGGTGCTGTTTACTA+CGG 0.549078 4_1:+68786049 Msa0567440:CDS
GACCTTACAGCACAATGAAG+AGG 0.556668 4_1:+68783704 Msa0567440:five_prime_UTR
GTTAATCCTGCATAGAGAGC+AGG 0.556840 4_1:-68786346 None:intergenic
TTTGACAACATACACTGTCT+AGG 0.558995 4_1:+68787247 Msa0567440:three_prime_UTR
TCGGCCACCTCTTAGGTCCA+TGG 0.563547 4_1:-68787202 None:intergenic
TGGAATATCAGGAAAAGGGT+CGG 0.564414 4_1:+68784071 Msa0567440:CDS
CGCCATATGATCAAAACTGA+GGG 0.565763 4_1:+68785977 Msa0567440:CDS
ACCACTTGAGAGACTTAAGC+TGG 0.566075 4_1:+68784998 Msa0567440:CDS
AAGCTGGAATACATAGTTCG+TGG 0.567190 4_1:+68785014 Msa0567440:CDS
AGTAGTATGCAGGATGTCCA+AGG 0.568282 4_1:-68787417 None:intergenic
ACTCCAATATATGAAAGAAG+AGG 0.568847 4_1:+68786126 Msa0567440:CDS
TCCGTTTATAGACAAACTCA+AGG 0.575667 4_1:-68784141 None:intergenic
CTCCAATATATGAAAGAAGA+GGG 0.575719 4_1:+68786127 Msa0567440:CDS
TCTTGCAAGATAACAGAGGA+GGG 0.579967 4_1:+68786727 Msa0567440:CDS
TAGAATTGGATGAAGAAGAT+GGG 0.581434 4_1:-68784028 None:intergenic
GTCCATAGGCAAGCAGAGCA+AGG 0.582869 4_1:-68785264 None:intergenic
TTAGGACACTAGTAGTATGC+AGG 0.583138 4_1:-68787427 None:intergenic
GATTCCTTCTTCTTTCCAGG+TGG 0.584016 4_1:+68783954 Msa0567440:CDS
TTTGCACTCACAGTGTCCAT+AGG 0.584166 4_1:-68785278 None:intergenic
TTCTTGCAAGATAACAGAGG+AGG 0.585830 4_1:+68786726 Msa0567440:CDS
AGCAGTAGGAATCTAACCCT+TGG 0.586003 4_1:+68787400 Msa0567440:three_prime_UTR
GCATATCTGCATTCACCCGA+GGG 0.587818 4_1:+68786094 Msa0567440:CDS
GGTTATACCTGCAATAAGCT+GGG 0.590199 4_1:-68786370 None:intergenic
AATTTATCAAAGTTGGAACA+TGG 0.590205 4_1:+68786521 Msa0567440:CDS
TGAAGAAGACCTTGAGACCA+TGG 0.593221 4_1:-68784285 None:intergenic
GCAATGCTTTGATGAATAGG+AGG 0.599931 4_1:+68787291 Msa0567440:three_prime_UTR
GAAGGAGGTAGGAACAGTGT+TGG 0.612519 4_1:-68783991 None:intergenic
TGAAGAAGATGGGATGCTGC+AGG 0.613995 4_1:-68784018 None:intergenic
GTCCACACCATGGACCTAAG+AGG 0.616241 4_1:+68787195 Msa0567440:three_prime_UTR
TGTGTCAAGATTGTTGAGCA+AGG 0.616757 4_1:+68786316 Msa0567440:CDS
AGCATATCTGCATTCACCCG+AGG 0.623364 4_1:+68786093 Msa0567440:CDS
AAGTAACTAATAGCAGCAGA+TGG 0.625598 4_1:-68787053 None:intergenic
GTCCATGGTGTGGACACAAG+TGG 0.626040 4_1:-68787187 None:intergenic
TGTGCTGTAAGGTCTGTTGG+TGG 0.626594 4_1:-68783695 None:intergenic
CTGCAGGTAATGAAGGAGGT+AGG 0.627394 4_1:-68784002 None:intergenic
TATATGTCGAGTTGTCGACC+AGG 0.633214 4_1:+68786762 Msa0567440:CDS
AGTACCCTCCATTATCAGCA+TGG 0.633664 4_1:+68786024 Msa0567440:CDS
CTCCACTTGTGTCCACACCA+TGG 0.634128 4_1:+68787185 Msa0567440:three_prime_UTR
CCGCCATATGATCAAAACTG+AGG 0.638358 4_1:+68785976 Msa0567440:CDS
TCCAGCTTAAGTCTCTCAAG+TGG 0.638459 4_1:-68784999 None:intergenic
CCGTAGTAAACAGCACCTGA+AGG 0.642418 4_1:-68786049 None:intergenic
AGGACAGTAATGGTAGCACC+TGG 0.645057 4_1:+68785920 Msa0567440:CDS
CGGCAAAAGCACCAGACCAG+AGG 0.651558 4_1:-68784262 None:intergenic
ACAGCACAATGAAGAGGCGA+CGG 0.657096 4_1:+68783710 Msa0567440:five_prime_UTR
AGGTGCCATGCTGATAATGG+AGG 0.661611 4_1:-68786029 None:intergenic
GCACAATGAAGAGGCGACGG+TGG 0.662699 4_1:+68783713 Msa0567440:five_prime_UTR
TTAGGTCCAAAATACAACGT+TGG 0.669579 4_1:-68786500 None:intergenic
GTAGTATGCAGGATGTCCAA+GGG 0.669681 4_1:-68787416 None:intergenic
CACCTCTTAGGTCCATGGTG+TGG 0.679141 4_1:-68787197 None:intergenic
CCTGGCGGTGAAGCATTGGG+AGG 0.688081 4_1:+68785938 Msa0567440:CDS
CACACCATGGACCTAAGAGG+TGG 0.690827 4_1:+68787198 Msa0567440:three_prime_UTR
ATAAACTAGTCAGATTATCG+GGG 0.695810 4_1:+68785180 Msa0567440:CDS
GATGCTGCAGGTAATGAAGG+AGG 0.698778 4_1:-68784006 None:intergenic
TGCAAGATAACAGAGGAGGG+AGG 0.712404 4_1:+68786730 Msa0567440:CDS
GGTGCCATGCTGATAATGGA+GGG 0.735104 4_1:-68786028 None:intergenic
GTCAAGATTGTTGAGCAAGG+AGG 0.739447 4_1:+68786319 Msa0567440:CDS
ACAGTAATGGTAGCACCTGG+CGG 0.741224 4_1:+68785923 Msa0567440:CDS
AGAAGACCTTGAGACCATGG+CGG 0.787349 4_1:-68784282 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTATATGTATCAT+AGG - chr4_1:68785165-68785184 None:intergenic 10.0%
!! TAGAAAAAGAAAAAAAAAAT+GGG - chr4_1:68785522-68785541 None:intergenic 10.0%
!!! CATTCATTCATTTTATTTAT+AGG + chr4_1:68784430-68784449 Msa0567440:intron 15.0%
!! AAGTAAATGAAAATAATAAG+TGG - chr4_1:68784721-68784740 None:intergenic 15.0%
!!! TAAAAATTTATAGCCTTAAA+GGG - chr4_1:68785140-68785159 None:intergenic 15.0%
!!! ATAAAAATTTATAGCCTTAA+AGG - chr4_1:68785141-68785160 None:intergenic 15.0%
!!! ACTTTCAATTTTCAAAATAT+TGG + chr4_1:68785476-68785495 Msa0567440:intron 15.0%
!!! CTTTCAATTTTCAAAATATT+GGG + chr4_1:68785477-68785496 Msa0567440:intron 15.0%
!!! TTTCAATTTTCAAAATATTG+GGG + chr4_1:68785478-68785497 Msa0567440:intron 15.0%
!! CTAGAAAAAGAAAAAAAAAA+TGG - chr4_1:68785523-68785542 None:intergenic 15.0%
!!! TTTTTTTCTTTTTCTAGTTT+CGG + chr4_1:68785526-68785545 Msa0567440:intron 15.0%
!!! TTATTAAAGGAGTTTTAAAT+AGG - chr4_1:68785746-68785765 None:intergenic 15.0%
!! TTAATGATATGAATTCTATA+AGG - chr4_1:68786923-68786942 None:intergenic 15.0%
!! TTATAGAATTCATATCATTA+AGG + chr4_1:68786922-68786941 Msa0567440:intron 15.0%
!!! CATTGTTTTTTATTTGAGTT+TGG - chr4_1:68783907-68783926 None:intergenic 20.0%
!! GATATTTATATGAGAATCAA+TGG - chr4_1:68784404-68784423 None:intergenic 20.0%
!! TAATATGAATCAAAGTAAAC+TGG - chr4_1:68784778-68784797 None:intergenic 20.0%
!! AGTTTACTTTGATTCATATT+AGG + chr4_1:68784777-68784796 Msa0567440:intron 20.0%
!!! GTTTTTTAAAAAGAAAAGAC+AGG - chr4_1:68784848-68784867 None:intergenic 20.0%
!! GTGTATTGTATTAATTATGT+TGG + chr4_1:68784955-68784974 Msa0567440:intron 20.0%
!!! ATTAATTATGTTGGTTTTTC+AGG + chr4_1:68784964-68784983 Msa0567440:intron 20.0%
!! AAATAAACTAGTCAGATTAT+CGG + chr4_1:68785178-68785197 Msa0567440:CDS 20.0%
!! ATATGTGTTTAAATGTTGTT+TGG + chr4_1:68785340-68785359 Msa0567440:intron 20.0%
!! TTTGAAAATTGAAAGTCATT+GGG - chr4_1:68785472-68785491 None:intergenic 20.0%
!!! TTTTGAAAATTGAAAGTCAT+TGG - chr4_1:68785473-68785492 None:intergenic 20.0%
!! AAACTATATGATAAGTTTAC+TGG + chr4_1:68785621-68785640 Msa0567440:intron 20.0%
!! AACTATATGATAAGTTTACT+GGG + chr4_1:68785622-68785641 Msa0567440:intron 20.0%
!! ACATGATGTTAATAATTTAG+TGG + chr4_1:68785686-68785705 Msa0567440:intron 20.0%
!! TGTTAATAATTTAGTGGTTA+TGG + chr4_1:68785692-68785711 Msa0567440:intron 20.0%
!! AAACTCCTTTAATAATTTAG+TGG + chr4_1:68785751-68785770 Msa0567440:intron 20.0%
!! ACCATTAGAACTTTATTATA+TGG + chr4_1:68786187-68786206 Msa0567440:CDS 20.0%
!!! ACCATATAATAAAGTTCTAA+TGG - chr4_1:68786191-68786210 None:intergenic 20.0%
!! AAGTATATGTTGAAAATAAG+AGG - chr4_1:68786394-68786413 None:intergenic 20.0%
!! AATTTATTGTACAATTTGCA+CGG - chr4_1:68786421-68786440 None:intergenic 20.0%
!! TTATAAAATGTGTGAGTAAT+CGG - chr4_1:68787224-68787243 None:intergenic 20.0%
!!! TTTTTATAGACAACTATTAG+CGG + chr4_1:68787348-68787367 Msa0567440:three_prime_UTR 20.0%
! ATGTCTCGTGTTTATTTAAT+TGG - chr4_1:68783752-68783771 None:intergenic 25.0%
!!! TGTTAGCACTACAATTTTTT+AGG + chr4_1:68784493-68784512 Msa0567440:intron 25.0%
!!! ACTACAATTTTTTAGGAGAA+AGG + chr4_1:68784500-68784519 Msa0567440:intron 25.0%
!!! CTACAATTTTTTAGGAGAAA+GGG + chr4_1:68784501-68784520 Msa0567440:intron 25.0%
!!! TACCAAAGATTTTTTCAACT+AGG + chr4_1:68784571-68784590 Msa0567440:intron 25.0%
!!! ACCAAAGATTTTTTCAACTA+GGG + chr4_1:68784572-68784591 Msa0567440:intron 25.0%
! ATGATAATAGTGCATATGAA+TGG - chr4_1:68784922-68784941 None:intergenic 25.0%
! ATGCACTATTATCATAAAGA+TGG + chr4_1:68784927-68784946 Msa0567440:intron 25.0%
! TATTATCATAAAGATGGTGA+CGG + chr4_1:68784933-68784952 Msa0567440:intron 25.0%
!! TTTTGTGAATATTCTTCGAA+CGG + chr4_1:68785112-68785131 Msa0567440:CDS 25.0%
! AATAAACTAGTCAGATTATC+GGG + chr4_1:68785179-68785198 Msa0567440:CDS 25.0%
! AACAAATCTCTCAAAATTTG+TGG - chr4_1:68785216-68785235 None:intergenic 25.0%
! AAAATAATATCAGAGCTAAG+AGG - chr4_1:68785312-68785331 None:intergenic 25.0%
! TTTGACAAGTATGATATGAT+AGG + chr4_1:68785373-68785392 Msa0567440:intron 25.0%
! AGATTAGGAAATAAGAAAAG+AGG - chr4_1:68785597-68785616 None:intergenic 25.0%
!!! TCATATAGTTTTCTGAGATT+AGG - chr4_1:68785612-68785631 None:intergenic 25.0%
! TCATGTGAAAAAGAAATAGT+TGG - chr4_1:68785672-68785691 None:intergenic 25.0%
! AGGAACCACTAAATTATTAA+AGG - chr4_1:68785759-68785778 None:intergenic 25.0%
! AAACTAACATATCCACTAAA+TGG - chr4_1:68785817-68785836 None:intergenic 25.0%
! AAACACTAGTATATTTCAGT+AGG - chr4_1:68785847-68785866 None:intergenic 25.0%
!!! TTTATTATATGGTGCTATTG+CGG + chr4_1:68786198-68786217 Msa0567440:CDS 25.0%
! TGTTCCAACTTTGATAAATT+AGG - chr4_1:68786521-68786540 None:intergenic 25.0%
! AATTTATCAAAGTTGGAACA+TGG + chr4_1:68786521-68786540 Msa0567440:CDS 25.0%
!!! ATCGTACAATTTTGATATGA+AGG + chr4_1:68786544-68786563 Msa0567440:CDS 25.0%
! TAAAATTGTGTTCTTGTGTA+TGG + chr4_1:68786642-68786661 Msa0567440:intron 25.0%
! ATTGTATAAAATCCATCCTA+TGG - chr4_1:68786825-68786844 None:intergenic 25.0%
! GATCAGACAATTATAATTGA+CGG - chr4_1:68786983-68787002 None:intergenic 25.0%
! TTGAAACAACAGATATAGTT+TGG + chr4_1:68787142-68787161 Msa0567440:three_prime_UTR 25.0%
TTGGCTTATTAAGAAGAAGA+AGG - chr4_1:68783833-68783852 None:intergenic 30.0%
!! TTTTATTTGAGTTTGGTACG+AGG - chr4_1:68783900-68783919 None:intergenic 30.0%
!!! ATCGTTTTGTTTGATCAAGT+GGG - chr4_1:68783931-68783950 None:intergenic 30.0%
TAGAATTGGATGAAGAAGAT+GGG - chr4_1:68784031-68784050 None:intergenic 30.0%
TTTCTTGGAATATCAGGAAA+AGG + chr4_1:68784066-68784085 Msa0567440:CDS 30.0%
TTCTTGGAATATCAGGAAAA+GGG + chr4_1:68784067-68784086 Msa0567440:CDS 30.0%
! TTTGAGATTCTTAAGGTTCA+AGG + chr4_1:68784095-68784114 Msa0567440:CDS 30.0%
TCCTTGAGTTTGTCTATAAA+CGG + chr4_1:68784140-68784159 Msa0567440:CDS 30.0%
ATCCAATCAAGTTTCAAAGA+AGG + chr4_1:68784193-68784212 Msa0567440:CDS 30.0%
TTCAAAGAAGGAGAATATTC+AGG + chr4_1:68784205-68784224 Msa0567440:CDS 30.0%
GTCTCAATTACAGATTATCA+TGG - chr4_1:68784528-68784547 None:intergenic 30.0%
TCCCTAGTTGAAAAAATCTT+TGG - chr4_1:68784576-68784595 None:intergenic 30.0%
TCACTGAGAATTACAAAAGT+TGG + chr4_1:68784692-68784711 Msa0567440:intron 30.0%
CACTGAGAATTACAAAAGTT+GGG + chr4_1:68784693-68784712 Msa0567440:intron 30.0%
!! GAAGCTTTAATGTACAGTTT+TGG + chr4_1:68784819-68784838 Msa0567440:intron 30.0%
TGCATTTCATTTATTTCACC+CGG - chr4_1:68784886-68784905 None:intergenic 30.0%
ATAAACTAGTCAGATTATCG+GGG + chr4_1:68785180-68785199 Msa0567440:CDS 30.0%
!!! AATTTTGAGAGATTTGTTGC+TGG + chr4_1:68785218-68785237 Msa0567440:CDS 30.0%
GATATGATAGGTATGAATCA+AGG + chr4_1:68785385-68785404 Msa0567440:intron 30.0%
! TGTTGTTTGTAGACTGATTT+GGG + chr4_1:68785426-68785445 Msa0567440:intron 30.0%
! TCAAACCATTGGTTAAACTA+AGG - chr4_1:68785779-68785798 None:intergenic 30.0%
TAACATATCCACTAAATGGT+AGG - chr4_1:68785813-68785832 None:intergenic 30.0%
ACTCCAATATATGAAAGAAG+AGG + chr4_1:68786126-68786145 Msa0567440:CDS 30.0%
CTCCAATATATGAAAGAAGA+GGG + chr4_1:68786127-68786146 Msa0567440:CDS 30.0%
!! TTATTATATGGTGCTATTGC+GGG + chr4_1:68786199-68786218 Msa0567440:CDS 30.0%
AAATCATCTAATCTGGATCA+TGG - chr4_1:68786475-68786494 None:intergenic 30.0%
TGGACCTAATTTATCAAAGT+TGG + chr4_1:68786514-68786533 Msa0567440:CDS 30.0%
!! TTTACACTGCTTTTATTGCA+TGG - chr4_1:68786626-68786645 None:intergenic 30.0%
! GACGGTGTAAAACTTTTTAA+CGG - chr4_1:68786965-68786984 None:intergenic 30.0%
GAGAATATTCTCATCTTCAA+TGG - chr4_1:68787168-68787187 None:intergenic 30.0%
!! AGAGATAATTTGTTAGCAGT+AGG + chr4_1:68787386-68787405 Msa0567440:three_prime_UTR 30.0%
TGTGAAAAAGAGAATGAGAG+AGG - chr4_1:68783777-68783796 None:intergenic 35.0%
!! TTTTTCACACGTTGTTGTTG+TGG - chr4_1:68783857-68783876 None:intergenic 35.0%
!!! CATCGTTTTGTTTGATCAAG+TGG - chr4_1:68783932-68783951 None:intergenic 35.0%
GTAGAATTGGATGAAGAAGA+TGG - chr4_1:68784032-68784051 None:intergenic 35.0%
CCTGATATTCCAAGAAAAAC+AGG - chr4_1:68784063-68784082 None:intergenic 35.0%
!! CCTGTTTTTCTTGGAATATC+AGG + chr4_1:68784060-68784079 Msa0567440:CDS 35.0%
TCCGTTTATAGACAAACTCA+AGG - chr4_1:68784144-68784163 None:intergenic 35.0%
!! CTCCTTCTTTGAAACTTGAT+TGG - chr4_1:68784198-68784217 None:intergenic 35.0%
! AAGGAGAATATTCAGGTTCA+TGG + chr4_1:68784212-68784231 Msa0567440:CDS 35.0%
! GAATATTCAGGTTCATGGAT+CGG + chr4_1:68784217-68784236 Msa0567440:CDS 35.0%
! AATATTCAGGTTCATGGATC+GGG + chr4_1:68784218-68784237 Msa0567440:CDS 35.0%
TGGTGTGACAAAATATAGCA+GGG - chr4_1:68784384-68784403 None:intergenic 35.0%
ATGGTGTGACAAAATATAGC+AGG - chr4_1:68784385-68784404 None:intergenic 35.0%
TGACTCATATCATAGACCAA+AGG + chr4_1:68784459-68784478 Msa0567440:intron 35.0%
GACTCATATCATAGACCAAA+GGG + chr4_1:68784460-68784479 Msa0567440:intron 35.0%
TAACAAAGAACTTGTCCCTT+TGG - chr4_1:68784478-68784497 None:intergenic 35.0%
TGTAATTCTCAGTGACCAAA+TGG - chr4_1:68784687-68784706 None:intergenic 35.0%
! CTCAAGGTTTGAAAGGATTT+TGG + chr4_1:68785081-68785100 Msa0567440:CDS 35.0%
!! GGTTTGAAAGGATTTTGGAA+AGG + chr4_1:68785086-68785105 Msa0567440:CDS 35.0%
AAACAACACCACAATGCATA+AGG - chr4_1:68785414-68785433 None:intergenic 35.0%
!! GTGTTGTTTGTAGACTGATT+TGG + chr4_1:68785425-68785444 Msa0567440:intron 35.0%
AAAATTGAAAGTCATTGGGC+AGG - chr4_1:68785468-68785487 None:intergenic 35.0%
TCGGTACTTTATGTTAACTC+TGG + chr4_1:68785545-68785564 Msa0567440:intron 35.0%
TGGTTCCTTAGTTTAACCAA+TGG + chr4_1:68785771-68785790 Msa0567440:intron 35.0%
AGAGCAATTTCTTTCAGATC+AGG + chr4_1:68785900-68785919 Msa0567440:intron 35.0%
! GGCTTCTTTTCTCTTTACAA+AGG + chr4_1:68785998-68786017 Msa0567440:CDS 35.0%
GACCCTCTTCTTTCATATAT+TGG - chr4_1:68786132-68786151 None:intergenic 35.0%
!! CATATCCCTTTGAAGTTGTA+AGG + chr4_1:68786242-68786261 Msa0567440:CDS 35.0%
TGTCTCCTTACAACTTCAAA+GGG - chr4_1:68786250-68786269 None:intergenic 35.0%
GGAACAGAAATCATCTAATC+TGG - chr4_1:68786482-68786501 None:intergenic 35.0%
! TCTGTTCCAACGTTGTATTT+TGG + chr4_1:68786494-68786513 Msa0567440:CDS 35.0%
TTAGGTCCAAAATACAACGT+TGG - chr4_1:68786503-68786522 None:intergenic 35.0%
AATTGTGTTCTTGTGTATGG+AGG + chr4_1:68786645-68786664 Msa0567440:intron 35.0%
GTCAACACTTGGAATTTCAT+GGG - chr4_1:68786680-68786699 None:intergenic 35.0%
AGTCAACACTTGGAATTTCA+TGG - chr4_1:68786681-68786700 None:intergenic 35.0%
TTGCATAGATAGTCAACACT+TGG - chr4_1:68786691-68786710 None:intergenic 35.0%
CTCTGTTATCTTGCAAGAAA+AGG - chr4_1:68786725-68786744 None:intergenic 35.0%
! CTTTTCTTGCAAGATAACAG+AGG + chr4_1:68786723-68786742 Msa0567440:CDS 35.0%
AAATCCATCCTATGGACTTT+GGG - chr4_1:68786817-68786836 None:intergenic 35.0%
AAAATCCATCCTATGGACTT+TGG - chr4_1:68786818-68786837 None:intergenic 35.0%
TTCTTCATCTTCGACCATAT+AGG + chr4_1:68786871-68786890 Msa0567440:intron 35.0%
TTCTATAAGGATTGCTACCA+GGG - chr4_1:68786910-68786929 None:intergenic 35.0%
ATTCTATAAGGATTGCTACC+AGG - chr4_1:68786911-68786930 None:intergenic 35.0%
!! CGTAACTGCTTCTATTTTTC+AGG + chr4_1:68787025-68787044 Msa0567440:intron 35.0%
AAGTAACTAATAGCAGCAGA+TGG - chr4_1:68787056-68787075 None:intergenic 35.0%
TTTGACAACATACACTGTCT+AGG + chr4_1:68787247-68787266 Msa0567440:three_prime_UTR 35.0%
TAGTGTCCTAACCATATCAT+TGG + chr4_1:68787439-68787458 Msa0567440:three_prime_UTR 35.0%
CGCATTTCATTCCTTTCGAT+CGG + chr4_1:68783802-68783821 Msa0567440:five_prime_UTR 40.0%
GATGATTCCTTCTTCTTTCC+AGG + chr4_1:68783951-68783970 Msa0567440:CDS 40.0%
ACAGGAAACAGTGGTAGAAT+TGG - chr4_1:68784045-68784064 None:intergenic 40.0%
CCAAGAAAAACAGGAAACAG+TGG - chr4_1:68784054-68784073 None:intergenic 40.0%
!! CCACTGTTTCCTGTTTTTCT+TGG + chr4_1:68784051-68784070 Msa0567440:CDS 40.0%
TGGAATATCAGGAAAAGGGT+CGG + chr4_1:68784071-68784090 Msa0567440:CDS 40.0%
!!! GGTCGGTTTTGAGATTCTTA+AGG + chr4_1:68784088-68784107 Msa0567440:CDS 40.0%
ATTCTTAAGGTTCAAGGTGC+TGG + chr4_1:68784101-68784120 Msa0567440:CDS 40.0%
TAGCAGGGTAGAACAAATTG+TGG - chr4_1:68784369-68784388 None:intergenic 40.0%
CACATCACATGTATCCAGTT+CGG - chr4_1:68784599-68784618 None:intergenic 40.0%
ACGATTTGAGTCCAACCATT+TGG + chr4_1:68784669-68784688 Msa0567440:intron 40.0%
ATTCTCAGTGACCAAATGGT+TGG - chr4_1:68784683-68784702 None:intergenic 40.0%
AAACGTTTCCAATCAGAAGC+CGG + chr4_1:68784864-68784883 Msa0567440:intron 40.0%
AAGCTGGAATACATAGTTCG+TGG + chr4_1:68785014-68785033 Msa0567440:CDS 40.0%
CAGACAATTGCAACTTCTCA+AGG + chr4_1:68785065-68785084 Msa0567440:CDS 40.0%
GCAACTTCTCAAGGTTTGAA+AGG + chr4_1:68785074-68785093 Msa0567440:CDS 40.0%
TAGTGGTTATGGTAGTTTCC+AGG + chr4_1:68785703-68785722 Msa0567440:intron 40.0%
AGTGGTTATGGTAGTTTCCA+GGG + chr4_1:68785704-68785723 Msa0567440:intron 40.0%
AGGTGCGTTATTCAAACCAT+TGG - chr4_1:68785790-68785809 None:intergenic 40.0%
CATATCCACTAAATGGTAGG+AGG - chr4_1:68785810-68785829 None:intergenic 40.0%
CTTTCAGATCAGGACAGTAA+TGG + chr4_1:68785910-68785929 Msa0567440:intron 40.0%
!! CCTCAGTTTTGATCATATGG+CGG - chr4_1:68785979-68785998 None:intergenic 40.0%
CGCCATATGATCAAAACTGA+GGG + chr4_1:68785977-68785996 Msa0567440:CDS 40.0%
!! AGCCCTCAGTTTTGATCATA+TGG - chr4_1:68785982-68786001 None:intergenic 40.0%
! TTGGAGTCTTTTCATTCCCT+CGG - chr4_1:68786113-68786132 None:intergenic 40.0%
!! TGCTTTGGAACAACTGGAAT+TGG + chr4_1:68786162-68786181 Msa0567440:CDS 40.0%
!! GCTTTGGAACAACTGGAATT+GGG + chr4_1:68786163-68786182 Msa0567440:CDS 40.0%
CTGTCTCCTTACAACTTCAA+AGG - chr4_1:68786251-68786270 None:intergenic 40.0%
ACAGCTTCAATTGCAGGTTA+GGG + chr4_1:68786267-68786286 Msa0567440:CDS 40.0%
TGTGTCAAGATTGTTGAGCA+AGG + chr4_1:68786316-68786335 Msa0567440:CDS 40.0%
ATTAACTCCCAGCTTATTGC+AGG + chr4_1:68786363-68786382 Msa0567440:CDS 40.0%
GGTTATACCTGCAATAAGCT+GGG - chr4_1:68786373-68786392 None:intergenic 40.0%
AGGTTATACCTGCAATAAGC+TGG - chr4_1:68786374-68786393 None:intergenic 40.0%
TTCTTGCAAGATAACAGAGG+AGG + chr4_1:68786726-68786745 Msa0567440:CDS 40.0%
TCTTGCAAGATAACAGAGGA+GGG + chr4_1:68786727-68786746 Msa0567440:CDS 40.0%
CAAATAATCCAAACCACACC+TGG - chr4_1:68786783-68786802 None:intergenic 40.0%
!! CTATGGACTTTGGGATTCTT+TGG - chr4_1:68786808-68786827 None:intergenic 40.0%
CAAAGAATCCCAAAGTCCAT+AGG + chr4_1:68786806-68786825 Msa0567440:intron 40.0%
GACCATATAGGACAAGTACT+GGG + chr4_1:68786883-68786902 Msa0567440:intron 40.0%
! ACCATATAGGACAAGTACTG+GGG + chr4_1:68786884-68786903 Msa0567440:intron 40.0%
ATACACTGTCTAGGTCTCAT+TGG + chr4_1:68787256-68787275 Msa0567440:three_prime_UTR 40.0%
CTGGCAATGCTTTGATGAAT+AGG + chr4_1:68787288-68787307 Msa0567440:three_prime_UTR 40.0%
GCAATGCTTTGATGAATAGG+AGG + chr4_1:68787291-68787310 Msa0567440:three_prime_UTR 40.0%
TTAGGACACTAGTAGTATGC+AGG - chr4_1:68787430-68787449 None:intergenic 40.0%
TGTCGTCCAATGATATGGTT+AGG - chr4_1:68787448-68787467 None:intergenic 40.0%
GAAGATGTCGTCCAATGATA+TGG - chr4_1:68787453-68787472 None:intergenic 40.0%
CATTGTGCTGTAAGGTCTGT+TGG - chr4_1:68783701-68783720 None:intergenic 45.0%
GACCTTACAGCACAATGAAG+AGG + chr4_1:68783704-68783723 Msa0567440:five_prime_UTR 45.0%
! CACACGTTGTTGTTGTGGTT+TGG - chr4_1:68783852-68783871 None:intergenic 45.0%
GATTCCTTCTTCTTTCCAGG+TGG + chr4_1:68783954-68783973 Msa0567440:CDS 45.0%
AAGACCACCTGGAAAGAAGA+AGG - chr4_1:68783961-68783980 None:intergenic 45.0%
TCAACATGACCAAACACCTC+TGG + chr4_1:68784246-68784265 Msa0567440:CDS 45.0%
TGAAGAAGACCTTGAGACCA+TGG - chr4_1:68784288-68784307 None:intergenic 45.0%
! TTTTCAACTAGGGACCGAAC+TGG + chr4_1:68784582-68784601 Msa0567440:intron 45.0%
AACGTTTCCAATCAGAAGCC+GGG + chr4_1:68784865-68784884 Msa0567440:intron 45.0%
TATTTCACCCGGCTTCTGAT+TGG - chr4_1:68784875-68784894 None:intergenic 45.0%
ACCACTTGAGAGACTTAAGC+TGG + chr4_1:68784998-68785017 Msa0567440:CDS 45.0%
TCCAGCTTAAGTCTCTCAAG+TGG - chr4_1:68785002-68785021 None:intergenic 45.0%
TTTGCACTCACAGTGTCCAT+AGG - chr4_1:68785281-68785300 None:intergenic 45.0%
CAAGGTTGCCTTATGCATTG+TGG + chr4_1:68785403-68785422 Msa0567440:intron 45.0%
GATTTGGGCAAACGTCACAT+AGG + chr4_1:68785441-68785460 Msa0567440:intron 45.0%
GATGTCAACGATTCAAACCC+TGG - chr4_1:68785724-68785743 None:intergenic 45.0%
!! GAAGCATTGGGAGGTGTAAT+CGG + chr4_1:68785947-68785966 Msa0567440:CDS 45.0%
CCGCCATATGATCAAAACTG+AGG + chr4_1:68785976-68785995 Msa0567440:CDS 45.0%
AGTACCCTCCATTATCAGCA+TGG + chr4_1:68786024-68786043 Msa0567440:CDS 45.0%
!! TGAAGGTGCCATGCTGATAA+TGG - chr4_1:68786035-68786054 None:intergenic 45.0%
ATTATCAGCATGGCACCTTC+AGG + chr4_1:68786034-68786053 Msa0567440:CDS 45.0%
!! CCTTCAGGTGCTGTTTACTA+CGG + chr4_1:68786049-68786068 Msa0567440:CDS 45.0%
! TGGAGTCTTTTCATTCCCTC+GGG - chr4_1:68786112-68786131 None:intergenic 45.0%
!! GCTGAATGCTTTGGAACAAC+TGG + chr4_1:68786156-68786175 Msa0567440:CDS 45.0%
AAGGAGACAGCTTCAATTGC+AGG + chr4_1:68786261-68786280 Msa0567440:CDS 45.0%
GACAGCTTCAATTGCAGGTT+AGG + chr4_1:68786266-68786285 Msa0567440:CDS 45.0%
GTCAAGATTGTTGAGCAAGG+AGG + chr4_1:68786319-68786338 Msa0567440:CDS 45.0%
GTTAATCCTGCATAGAGAGC+AGG - chr4_1:68786349-68786368 None:intergenic 45.0%
TCTAATCTGGATCATGGCTC+AGG - chr4_1:68786469-68786488 None:intergenic 45.0%
AATGTGTGATTCCCTTACCG+TGG + chr4_1:68786589-68786608 Msa0567440:intron 45.0%
ATTCCCTTACCGTGGTGAAT+CGG + chr4_1:68786597-68786616 Msa0567440:intron 45.0%
! TATATGTCGAGTTGTCGACC+AGG + chr4_1:68786762-68786781 Msa0567440:CDS 45.0%
GAATCCCAAAGTCCATAGGA+TGG + chr4_1:68786810-68786829 Msa0567440:intron 45.0%
CGACCATATAGGACAAGTAC+TGG + chr4_1:68786882-68786901 Msa0567440:intron 45.0%
GTCTCATTGGATTCTACTGC+TGG + chr4_1:68787269-68787288 Msa0567440:three_prime_UTR 45.0%
AGCAGTAGGAATCTAACCCT+TGG + chr4_1:68787400-68787419 Msa0567440:three_prime_UTR 45.0%
GTAGTATGCAGGATGTCCAA+GGG - chr4_1:68787419-68787438 None:intergenic 45.0%
AGTAGTATGCAGGATGTCCA+AGG - chr4_1:68787420-68787439 None:intergenic 45.0%
TGTGCTGTAAGGTCTGTTGG+TGG - chr4_1:68783698-68783717 None:intergenic 50.0%
CGCCTCTTCATTGTGCTGTA+AGG - chr4_1:68783709-68783728 None:intergenic 50.0%
ACAGCACAATGAAGAGGCGA+CGG + chr4_1:68783710-68783729 Msa0567440:five_prime_UTR 50.0%
! CGAGGTACGAGAAGTTGATC+TGG - chr4_1:68783882-68783901 None:intergenic 50.0%
GCGTGAAGAAAAAGACCACC+TGG - chr4_1:68783972-68783991 None:intergenic 50.0%
GAAGGAGGTAGGAACAGTGT+TGG - chr4_1:68783994-68784013 None:intergenic 50.0%
CTGCAGGTAATGAAGGAGGT+AGG - chr4_1:68784005-68784024 None:intergenic 50.0%
GATGCTGCAGGTAATGAAGG+AGG - chr4_1:68784009-68784028 None:intergenic 50.0%
TGGGATGCTGCAGGTAATGA+AGG - chr4_1:68784012-68784031 None:intergenic 50.0%
TGAAGAAGATGGGATGCTGC+AGG - chr4_1:68784021-68784040 None:intergenic 50.0%
ATGACCAAACACCTCTGGTC+TGG + chr4_1:68784251-68784270 Msa0567440:CDS 50.0%
AGAAGACCTTGAGACCATGG+CGG - chr4_1:68784285-68784304 None:intergenic 50.0%
TCTTCGAACGGCACCCTTTA+AGG + chr4_1:68785124-68785143 Msa0567440:CDS 50.0%
AACCTTGCTCTGCTTGCCTA+TGG + chr4_1:68785262-68785281 Msa0567440:CDS 50.0%
GACACTGTGAGTGCAAACTC+AGG + chr4_1:68785284-68785303 Msa0567440:intron 50.0%
ACGCACCTCCTACCATTTAG+TGG + chr4_1:68785802-68785821 Msa0567440:intron 50.0%
AGGACAGTAATGGTAGCACC+TGG + chr4_1:68785920-68785939 Msa0567440:CDS 50.0%
ACAGTAATGGTAGCACCTGG+CGG + chr4_1:68785923-68785942 Msa0567440:CDS 50.0%
!! GGTGCCATGCTGATAATGGA+GGG - chr4_1:68786031-68786050 None:intergenic 50.0%
!! AGGTGCCATGCTGATAATGG+AGG - chr4_1:68786032-68786051 None:intergenic 50.0%
CCGTAGTAAACAGCACCTGA+AGG - chr4_1:68786052-68786071 None:intergenic 50.0%
AGCATATCTGCATTCACCCG+AGG + chr4_1:68786093-68786112 Msa0567440:CDS 50.0%
GCATATCTGCATTCACCCGA+GGG + chr4_1:68786094-68786113 Msa0567440:CDS 50.0%
GGGTCAAGAGCTGAATGCTT+TGG + chr4_1:68786147-68786166 Msa0567440:CDS 50.0%
ATCTGGATCATGGCTCAGGT+AGG - chr4_1:68786465-68786484 None:intergenic 50.0%
TTCCCTTACCGTGGTGAATC+GGG + chr4_1:68786598-68786617 Msa0567440:intron 50.0%
ATCCCGATTCACCACGGTAA+GGG - chr4_1:68786603-68786622 None:intergenic 50.0%
TGCAAGATAACAGAGGAGGG+AGG + chr4_1:68786730-68786749 Msa0567440:CDS 50.0%
GCCCCAGTACTTGTCCTATA+TGG - chr4_1:68786888-68786907 None:intergenic 50.0%
TGAGTAATCGGCCACCTCTT+AGG - chr4_1:68787212-68787231 None:intergenic 50.0%
AGAAGGACGAGCCGATCGAA+AGG - chr4_1:68783816-68783835 None:intergenic 55.0%
!! AAGGTTCAAGGTGCTGGTGC+TGG + chr4_1:68784107-68784126 Msa0567440:CDS 55.0%
!! AGCACCAGACCAGAGGTGTT+TGG - chr4_1:68784258-68784277 None:intergenic 55.0%
! CTTTTGCCGCCATGGTCTCA+AGG + chr4_1:68784276-68784295 Msa0567440:CDS 55.0%
!! TTTGTTGCTGGTGCTGCTGC+AGG + chr4_1:68785230-68785249 Msa0567440:CDS 55.0%
!! TTGTTGCTGGTGCTGCTGCA+GGG + chr4_1:68785231-68785250 Msa0567440:CDS 55.0%
GTCCATAGGCAAGCAGAGCA+AGG - chr4_1:68785267-68785286 None:intergenic 55.0%
GGTGTTCCTGCTCTCTATGC+AGG + chr4_1:68786340-68786359 Msa0567440:CDS 55.0%
GATCCCGATTCACCACGGTA+AGG - chr4_1:68786604-68786623 None:intergenic 55.0%
GCATGGATCCCGATTCACCA+CGG - chr4_1:68786609-68786628 None:intergenic 55.0%
GTTGTCGACCAGGTGTGGTT+TGG + chr4_1:68786772-68786791 Msa0567440:intron 55.0%
CTCCACTTGTGTCCACACCA+TGG + chr4_1:68787185-68787204 Msa0567440:three_prime_UTR 55.0%
GTCCATGGTGTGGACACAAG+TGG - chr4_1:68787190-68787209 None:intergenic 55.0%
GTCCACACCATGGACCTAAG+AGG + chr4_1:68787195-68787214 Msa0567440:three_prime_UTR 55.0%
CACCTCTTAGGTCCATGGTG+TGG - chr4_1:68787200-68787219 None:intergenic 55.0%
CACACCATGGACCTAAGAGG+TGG + chr4_1:68787198-68787217 Msa0567440:three_prime_UTR 55.0%
GCACAATGAAGAGGCGACGG+TGG + chr4_1:68783713-68783732 Msa0567440:five_prime_UTR 60.0%
!! CGGCAAAAGCACCAGACCAG+AGG - chr4_1:68784265-68784284 None:intergenic 60.0%
! GTCTGGTGCTTTTGCCGCCA+TGG + chr4_1:68784268-68784287 Msa0567440:CDS 60.0%
!! TGTTGCTGGTGCTGCTGCAG+GGG + chr4_1:68785232-68785251 Msa0567440:CDS 60.0%
AGCACCTGGCGGTGAAGCAT+TGG + chr4_1:68785934-68785953 Msa0567440:CDS 60.0%
! GCACCTGGCGGTGAAGCATT+GGG + chr4_1:68785935-68785954 Msa0567440:CDS 60.0%
! GTCGAGTTGTCGACCAGGTG+TGG + chr4_1:68786767-68786786 Msa0567440:intron 60.0%
!! TAGGACAAGTACTGGGGCCC+TGG + chr4_1:68786890-68786909 Msa0567440:intron 60.0%
TCGGCCACCTCTTAGGTCCA+TGG - chr4_1:68787205-68787224 None:intergenic 60.0%
CCTCCCAATGCTTCACCGCC+AGG - chr4_1:68785941-68785960 None:intergenic 65.0%
!! CCTGGCGGTGAAGCATTGGG+AGG + chr4_1:68785938-68785957 Msa0567440:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 68783690 68787473 68783690 ID=Msa0567440;Name=Msa0567440
chr4_1 mRNA 68783690 68787473 68783690 ID=Msa0567440-mRNA-1;Parent=Msa0567440;Name=Msa0567440-mRNA-1;_AED=0.08;_eAED=0.08;_QI=234|0.4|0.66|1|1|1|6|352|471
chr4_1 exon 68783690 68784297 68783690 ID=Msa0567440-mRNA-1:exon:22805;Parent=Msa0567440-mRNA-1
chr4_1 exon 68784986 68785289 68784986 ID=Msa0567440-mRNA-1:exon:22806;Parent=Msa0567440-mRNA-1
chr4_1 exon 68785917 68786384 68785917 ID=Msa0567440-mRNA-1:exon:22807;Parent=Msa0567440-mRNA-1
chr4_1 exon 68786488 68786565 68786488 ID=Msa0567440-mRNA-1:exon:22808;Parent=Msa0567440-mRNA-1
chr4_1 exon 68786667 68786783 68786667 ID=Msa0567440-mRNA-1:exon:22809;Parent=Msa0567440-mRNA-1
chr4_1 exon 68787047 68787473 68787047 ID=Msa0567440-mRNA-1:exon:22810;Parent=Msa0567440-mRNA-1
chr4_1 five_prime_UTR 68783690 68783923 68783690 ID=Msa0567440-mRNA-1:five_prime_utr;Parent=Msa0567440-mRNA-1
chr4_1 CDS 68783924 68784297 68783924 ID=Msa0567440-mRNA-1:cds;Parent=Msa0567440-mRNA-1
chr4_1 CDS 68784986 68785289 68784986 ID=Msa0567440-mRNA-1:cds;Parent=Msa0567440-mRNA-1
chr4_1 CDS 68785917 68786384 68785917 ID=Msa0567440-mRNA-1:cds;Parent=Msa0567440-mRNA-1
chr4_1 CDS 68786488 68786565 68786488 ID=Msa0567440-mRNA-1:cds;Parent=Msa0567440-mRNA-1
chr4_1 CDS 68786667 68786783 68786667 ID=Msa0567440-mRNA-1:cds;Parent=Msa0567440-mRNA-1
chr4_1 CDS 68787047 68787121 68787047 ID=Msa0567440-mRNA-1:cds;Parent=Msa0567440-mRNA-1
chr4_1 three_prime_UTR 68787122 68787473 68787122 ID=Msa0567440-mRNA-1:three_prime_utr;Parent=Msa0567440-mRNA-1
Gene Sequence

>Msa0567440

ATGTCCCACTTGATCAAACAAAACGATGATGATTCCTTCTTCTTTCCAGGTGGTCTTTTTCTTCACGCCAACACTGTTCCTACCTCCTTCATTACCTGCAGCATCCCATCTTCTTCATCCAATTCTACCACTGTTTCCTGTTTTTCTTGGAATATCAGGAAAAGGGTCGGTTTTGAGATTCTTAAGGTTCAAGGTGCTGGTGCTGGTGTTTTCTCATCCTTGAGTTTGTCTATAAACGGAAGAAGCGACGATGTAGATCATAGATACAAATCCAATCAAGTTTCAAAGAAGGAGAATATTCAGGTTCATGGATCGGGTGCTGTCAACATGACCAAACACCTCTGGTCTGGTGCTTTTGCCGCCATGGTCTCAAGGACTTTTGTTGCACCACTTGAGAGACTTAAGCTGGAATACATAGTTCGTGGTGAACAGAAGAATCTTTTTGAGCTCATTCAGACAATTGCAACTTCTCAAGGTTTGAAAGGATTTTGGAAAGGAAATTTTGTGAATATTCTTCGAACGGCACCCTTTAAGGCTATAAATTTTTATGCCTATGATACATATAAAAATAAACTAGTCAGATTATCGGGGAATGAAGAATCCACAAATTTTGAGAGATTTGTTGCTGGTGCTGCTGCAGGGGTTACAGCAACCTTGCTCTGCTTGCCTATGGACACTATCAGGACAGTAATGGTAGCACCTGGCGGTGAAGCATTGGGAGGTGTAATCGGTGTCTTCCGCCATATGATCAAAACTGAGGGCTTCTTTTCTCTTTACAAAGGTTTAGTACCCTCCATTATCAGCATGGCACCTTCAGGTGCTGTTTACTACGGCGTTTATGATATTTTAAAGTCAGCATATCTGCATTCACCCGAGGGAATGAAAAGACTCCAATATATGAAAGAAGAGGGTCAAGAGCTGAATGCTTTGGAACAACTGGAATTGGGTACCATTAGAACTTTATTATATGGTGCTATTGCGGGTTGTTGTTCTGAAGCTGCTACATATCCCTTTGAAGTTGTAAGGAGACAGCTTCAATTGCAGGTTAGGGCAACAAGATTGAACGCACTAGCGACTTGTGTCAAGATTGTTGAGCAAGGAGGTGTTCCTGCTCTCTATGCAGGATTAACTCCCAGCTTATTGCAGATGATTTCTGTTCCAACGTTGTATTTTGGACCTAATTTATCAAAGTTGGAACATGGATCGTACAATTTTGATATGAAGGATAGCAATACCCATGAAATTCCAAGTGTTGACTATCTATGCAATCAAAAGATGTCCTTTTCTTGCAAGATAACAGAGGAGGGAGGAATTTATGTTATATGTCGAGTTGTCGACCAGGTGTTACCATCTGCTGCTATTAGTTACTTTGTATACGAGTTCATGAAGATAGTTCTCAAAGTAGAATCAACATAG
Protein sequence

>Msa0567440

MSHLIKQNDDDSFFFPGGLFLHANTVPTSFITCSIPSSSSNSTTVSCFSWNIRKRVGFEILKVQGAGAGVFSSLSLSINGRSDDVDHRYKSNQVSKKENIQVHGSGAVNMTKHLWSGAFAAMVSRTFVAPLERLKLEYIVRGEQKNLFELIQTIATSQGLKGFWKGNFVNILRTAPFKAINFYAYDTYKNKLVRLSGNEESTNFERFVAGAAAGVTATLLCLPMDTIRTVMVAPGGEALGGVIGVFRHMIKTEGFFSLYKGLVPSIISMAPSGAVYYGVYDILKSAYLHSPEGMKRLQYMKEEGQELNALEQLELGTIRTLLYGAIAGCCSEAATYPFEVVRRQLQLQVRATRLNALATCVKIVEQGGVPALYAGLTPSLLQMISVPTLYFGPNLSKLEHGSYNFDMKDSNTHEIPSVDYLCNQKMSFSCKITEEGGIYVICRVVDQVLPSAAISYFVYEFMKIVLKVEST*