AlfalfaGEDB | Msa0625780

M. sativa cultivar zhongmu-4 / Msa0625780

KEGG: K09753
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006725 GO:0006950 GO:0007623 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009409 GO:0009628 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009987 GO:0016491 GO:0016620 GO:0016621 GO:0016903 GO:0019438 GO:0019748 GO:0042752 GO:0042754 GO:0044237 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0048511 GO:0048519 GO:0050789 GO:0050896 GO:0055114 GO:0065007 GO:0071704 GO:1901360 GO:1901362 GO:1901576
PFAM: PF01370.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0625780	XP_003604238.1	98.810	336	4	0	1	336	1	336	0.0	689

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0625780	sp\|A0A059TC02\|CCR1_PETHY	75.460	326	78	1	9	334	3	326	0.0	521

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0013270	Msa0625780	0.817242	3.649862e-52	-8.615850e-47
Msa0037180	Msa0625780	0.811609	6.427043e-51	-8.615850e-47
Msa0045810	Msa0625780	0.809476	1.857061e-50	-8.615850e-47
Msa0062190	Msa0625780	0.820204	7.762802e-53	-8.615850e-47
Msa0072180	Msa0625780	0.815106	1.095890e-51	-8.615850e-47
Msa0081120	Msa0625780	-0.801703	7.944012e-49	-8.615850e-47
Msa0092220	Msa0625780	0.813844	2.083109e-51	-8.615850e-47
Msa0106540	Msa0625780	0.810005	1.428827e-50	-8.615850e-47
Msa0177320	Msa0625780	0.816528	5.278228e-52	-8.615850e-47
Msa0204950	Msa0625780	0.832283	1.032675e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0625780	MtrunA17_Chr4g0001321	98.810	336	4	0	1	336	1	336	0.0	689
Msa0625780	MtrunA17_Chr2g0333781	81.155	329	59	2	7	335	12	337	0.0	536
Msa0625780	MtrunA17_Chr3g0077221	51.242	322	148	6	9	323	2	321	2.18e-105	311
Msa0625780	MtrunA17_Chr3g0077231	50.159	315	148	6	16	323	5	317	5.34e-104	308
Msa0625780	MtrunA17_Chr4g0039311	48.125	320	157	6	11	323	5	322	9.67e-97	289
Msa0625780	MtrunA17_Chr5g0431311	46.519	316	162	3	15	324	3	317	1.92e-96	288
Msa0625780	MtrunA17_Chr8g0364051	47.531	324	160	6	10	326	1	321	1.19e-95	286
Msa0625780	MtrunA17_Chr4g0002661	46.296	324	162	4	11	326	4	323	2.29e-95	286
Msa0625780	MtrunA17_Chr4g0039321	47.500	320	159	7	11	323	3	320	6.94e-95	285
Msa0625780	MtrunA17_Chr8g0363831	47.352	321	159	6	10	323	2	319	3.67e-93	280

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0625780	AT1G80820.1	73.968	315	82	0	15	329	6	320	3.08e-171	479
Msa0625780	AT1G15950.3	72.871	317	86	0	10	326	6	322	3.39e-167	469
Msa0625780	AT1G15950.1	72.871	317	86	0	10	326	6	322	3.39e-167	469
Msa0625780	AT1G15950.2	72.168	309	86	0	10	318	6	314	1.22e-159	450
Msa0625780	AT1G80820.2	73.308	266	71	0	15	280	6	271	4.14e-138	393
Msa0625780	AT1G15950.6	72.222	270	75	0	11	280	7	276	1.63e-136	389
Msa0625780	AT1G15950.4	71.533	274	78	0	11	284	7	280	1.90e-136	389
Msa0625780	AT1G15950.5	72.119	269	75	0	11	279	7	275	9.67e-136	387
Msa0625780	AT1G15950.7	75.893	224	54	0	103	326	1	224	7.38e-115	333
Msa0625780	AT5G19440.1	51.090	321	148	6	11	324	4	322	3.58e-105	311

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Find 88 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAAATCAAGTTGATT+TGG	0.293241	4_3:+5086102	Msa0625780:CDS
AAAATAACTCACAGGGTTGT+CGG	0.470932	4_3:-5085607	None:intergenic
AAAGCTGAAAGACTTGGGAT+TGG	0.510048	4_3:+5087606	Msa0625780:CDS
AAATCAAAAGCTGAAAGACT+TGG	0.471600	4_3:+5087600	Msa0625780:CDS
AAATTTGCATACCTGGGTTT+CGG	0.363831	4_3:-5085219	None:intergenic
AACACTTACTTGGTAGGAAG+TGG	0.504048	4_3:-5086388	None:intergenic
AACACTTCATCAGTCTCTGG+TGG	0.644313	4_3:+5085100	Msa0625780:CDS
AACTGCTTTGCATCGTGGTG+AGG	0.603860	4_3:+5086330	Msa0625780:CDS
AAGCTGTGTGGAACACACCA+TGG	0.653770	4_3:-5085575	None:intergenic
AAGTGACACACTAAGCAATA+TGG	0.414623	4_3:-5084917	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATGATCACTTGAAAGAAT+TGG	+	chr4_3:5085473-5085492	Msa0625780:CDS	30.0%
CATATAATAGGATGAGATGT+TGG	+	chr4_3:5085720-5085739	Msa0625780:intron	30.0%
GTAGTGAATTAATCAAACCT+TGG	-	chr4_3:5085914-5085933	None:intergenic	30.0%
TTATGTTCATGTTAAGGATG+TGG	+	chr4_3:5086246-5086265	Msa0625780:CDS	30.0%
ATATGAAACACTTACTTGGT+AGG	-	chr4_3:5086397-5086416	None:intergenic	30.0%
GACTATATGAAACACTTACT+TGG	-	chr4_3:5086401-5086420	None:intergenic	30.0%
GCTTATGACTTATCTAGATT+AGG	+	chr4_3:5086469-5086488	Msa0625780:intron	30.0%
GAAAAAAACTTAGTTGAGAG+AGG	-	chr4_3:5086515-5086534	None:intergenic	30.0%
TGTACATCTAACTTGATCTT+GGG	+	chr4_3:5086677-5086696	Msa0625780:intron	30.0%
ATGACTTCTCCTAAAAATAG+TGG	-	chr4_3:5086970-5086989	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	5084799	5087897	5084799	ID=Msa0625780;Name=Msa0625780
chr4_3	mRNA	5084799	5087897	5084799	ID=Msa0625780-mRNA-1;Parent=Msa0625780;Name=Msa0625780-mRNA-1;_AED=0.24;_eAED=0.24;_QI=286\|1\|1\|1\|0.8\|0.66\|6\|148\|336
chr4_3	exon	5084799	5085229	5084799	ID=Msa0625780-mRNA-1:exon:1396;Parent=Msa0625780-mRNA-1
chr4_3	exon	5085463	5085617	5085463	ID=Msa0625780-mRNA-1:exon:1397;Parent=Msa0625780-mRNA-1
chr4_3	exon	5085730	5085915	5085730	ID=Msa0625780-mRNA-1:exon:1398;Parent=Msa0625780-mRNA-1
chr4_3	exon	5086049	5086401	5086049	ID=Msa0625780-mRNA-1:exon:1399;Parent=Msa0625780-mRNA-1
chr4_3	exon	5087555	5087790	5087555	ID=Msa0625780-mRNA-1:exon:1400;Parent=Msa0625780-mRNA-1
chr4_3	exon	5087814	5087897	5087814	ID=Msa0625780-mRNA-1:exon:1401;Parent=Msa0625780-mRNA-1
chr4_3	five_prime_UTR	5084799	5085084	5084799	ID=Msa0625780-mRNA-1:five_prime_utr;Parent=Msa0625780-mRNA-1
chr4_3	CDS	5085085	5085229	5085085	ID=Msa0625780-mRNA-1:cds;Parent=Msa0625780-mRNA-1
chr4_3	CDS	5085463	5085617	5085463	ID=Msa0625780-mRNA-1:cds;Parent=Msa0625780-mRNA-1
chr4_3	CDS	5085730	5085915	5085730	ID=Msa0625780-mRNA-1:cds;Parent=Msa0625780-mRNA-1
chr4_3	CDS	5086049	5086401	5086049	ID=Msa0625780-mRNA-1:cds;Parent=Msa0625780-mRNA-1
chr4_3	CDS	5087555	5087726	5087555	ID=Msa0625780-mRNA-1:cds;Parent=Msa0625780-mRNA-1
chr4_3	three_prime_UTR	5087727	5087790	5087727	ID=Msa0625780-mRNA-1:three_prime_utr;Parent=Msa0625780-mRNA-1
chr4_3	three_prime_UTR	5087814	5087897	5087814	ID=Msa0625780-mRNA-1:three_prime_utr;Parent=Msa0625780-mRNA-1

Gene Sequence

>Msa0625780

ATGCCTGCCTATGATAACACTTCATCAGTCTCTGGTGGCGACCAAATCGTGTGTGTCACCGGTGCTGGCGGGTTCATAGCTTCTTGGCTTGTCAAACTTCTCTTGGAAAGAGGTTACACTGTTAGAGGAACCGTCCGAAACCCAGAGGATCCAAAGAATGATCACTTGAAAGAATTGGAAGGAGCAAGAGAGAGGCTAACTTTGCATAAGGTTGATCTTCTTGATCTTCAATCTATCCAATCTGTTGTTCATGGTTGCCATGGTGTGTTCCACACAGCTTCTCCTGTCACCGACAACCCTGATGAGATGTTGGAGCCAGCAGTGAATGGAACAAAGAATGTGATTATAGCGTCTGCTGAAGCAAAAGTGCGACGTGTTGTGTTCACTTCATCGATTGGAACGGTCTATATGGACCCAAATACGAGTAGAGATGTTGTAGTTGATGAATCATATTGGAGTGATTTAGAGCATTGCAAGAACACCAAGAACTGGTATTGTTATGGGAAGACAGTGGCTGAACAATCAGCATGGGACATAGCAAAAGAAAATCAAGTTGATTTGGTTGTTGTGAACCCAGTTGTGGTTCTTGGACCATTACTTCAACCAACAATTAATGCTAGTACAATTCACATCCTCAAGTACCTTAATGGTGCTGCCAAAACTTATGTGAATGCAACACAATCTTATGTTCATGTTAAGGATGTGGCATTAGCACATCTTCTTGTTTATGAGACAAATTCTGCTTCTGGTAGATACATATGTTGTGAAACTGCTTTGCATCGTGGTGAGGTTGTTGAAATTTTGGCCAAGTATTTTCCTGAGTACCCACTTCCTACCAAATGTTCAGATGAAAAGAATCCAAGAGTGAAACCATACATATTCTCAAATCAAAAGCTGAAAGACTTGGGATTGGAATTCACACCTGTGAAGCAATGTTTATATGAAACTGTTAGGAGTCTACAAGAGAAAGGACACCTTCCTATTCCTCCCATGCAAGAAGACTCTGCTTAA

Protein sequence

>Msa0625780

MPAYDNTSSVSGGDQIVCVTGAGGFIASWLVKLLLERGYTVRGTVRNPEDPKNDHLKELEGARERLTLHKVDLLDLQSIQSVVHGCHGVFHTASPVTDNPDEMLEPAVNGTKNVIIASAEAKVRRVVFTSSIGTVYMDPNTSRDVVVDESYWSDLEHCKNTKNWYCYGKTVAEQSAWDIAKENQVDLVVVNPVVVLGPLLQPTINASTIHILKYLNGAAKTYVNATQSYVHVKDVALAHLLVYETNSASGRYICCETALHRGEVVEILAKYFPEYPLPTKCSDEKNPRVKPYIFSNQKLKDLGLEFTPVKQCLYETVRSLQEKGHLPIPPMQEDSA*