AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1407750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1407750 MtrunA17_Chr8g0382201 90.667 375 15 3 12 369 93 464 0.0 644
Msa1407750 MtrunA17_Chr3g0115801 59.737 380 115 7 11 369 23 385 1.27e-134 390
Msa1407750 MtrunA17_Chr1g0149111 35.145 276 138 5 115 369 137 392 8.13e-43 154
Msa1407750 MtrunA17_Chr1g0149111 30.270 185 106 4 186 369 132 294 9.79e-16 78.2
Msa1407750 MtrunA17_Chr1g0149111 26.230 183 110 4 111 270 227 407 3.00e-12 67.4
Msa1407750 MtrunA17_Chr3g0137941 34.058 276 141 5 115 369 120 375 5.98e-41 148
Msa1407750 MtrunA17_Chr3g0137941 31.868 182 101 4 189 369 118 277 8.31e-17 81.3
Msa1407750 MtrunA17_Chr3g0137941 24.373 279 181 5 21 278 129 398 4.69e-13 70.1
Msa1407750 MtrunA17_Chr4g0063561 34.058 276 141 5 115 369 121 376 2.05e-38 141
Msa1407750 MtrunA17_Chr4g0063561 29.670 182 106 4 189 369 119 279 5.65e-14 72.8
Msa1407750 MtrunA17_Chr7g0242311 30.275 327 155 12 85 368 41 337 2.00e-29 117
Msa1407750 MtrunA17_Chr7g0242311 27.861 201 121 4 108 291 178 371 8.63e-14 72.0
Msa1407750 MtrunA17_Chr7g0240341 26.846 298 159 6 111 369 19 296 1.72e-28 113
Msa1407750 MtrunA17_Chr4g0006851 31.852 270 125 11 128 368 100 339 1.11e-27 112
Msa1407750 MtrunA17_Chr4g0006851 26.943 193 117 4 116 291 188 373 2.75e-12 67.4
Msa1407750 MtrunA17_Chr5g0396801 28.788 330 172 8 89 369 5 320 1.70e-27 110
Msa1407750 MtrunA17_Chr5g0396801 30.137 146 83 3 145 271 191 336 2.15e-14 73.6
Msa1407750 MtrunA17_Chr7g0252401 31.338 284 147 11 111 369 201 461 7.02e-26 108
Msa1407750 MtrunA17_Chr7g0252401 24.719 178 110 3 117 272 303 478 1.26e-11 65.9
Msa1407750 MtrunA17_Chr8g0385571 30.333 300 162 11 96 369 206 484 1.63e-25 107
Msa1407750 MtrunA17_Chr8g0385571 25.000 180 110 3 117 271 321 500 7.44e-12 66.6
Msa1407750 MtrunA17_Chr8g0347451 28.852 305 155 8 112 369 46 335 2.38e-25 105
Msa1407750 MtrunA17_Chr8g0347451 28.378 148 85 3 145 271 204 351 1.16e-13 71.6
Msa1407750 MtrunA17_Chr3g0131501 29.568 301 163 11 96 369 184 462 1.89e-23 101
Msa1407750 MtrunA17_Chr3g0135861 26.645 304 149 7 115 369 33 311 6.84e-23 98.2
Msa1407750 MtrunA17_Chr2g0296061 23.920 301 166 7 119 368 17 305 8.21e-23 97.8
Msa1407750 MtrunA17_Chr2g0296061 28.105 153 89 3 144 276 175 326 2.75e-14 73.2
Msa1407750 MtrunA17_Chr4g0067231 24.590 305 161 8 119 368 16 306 1.14e-22 97.4
Msa1407750 MtrunA17_Chr4g0067231 27.922 154 90 3 143 276 175 327 3.26e-15 75.9
Msa1407750 MtrunA17_Chr6g0488081 26.531 294 155 7 115 369 17 288 2.86e-22 95.9
Msa1407750 MtrunA17_Chr8g0393031 29.930 284 138 10 123 367 24 285 3.21e-22 95.9
Msa1407750 MtrunA17_Chr5g0436041 25.676 296 163 6 111 369 28 303 2.50e-20 90.5
Msa1407750 MtrunA17_Chr4g0000261 23.208 293 166 7 115 369 64 335 7.97e-19 86.7
Msa1407750 MtrunA17_Chr3g0135801 24.458 323 181 10 86 368 4 303 4.34e-16 78.6
Msa1407750 MtrunA17_Chr8g0386871 23.547 327 194 9 73 367 265 567 2.06e-15 77.8
Msa1407750 MtrunA17_Chr8g0386871 28.090 178 106 5 116 273 412 587 1.39e-13 72.0
Msa1407750 MtrunA17_Chr8g0383321 23.127 307 199 9 76 369 5 287 4.11e-13 69.7
Msa1407750 MtrunA17_Chr8g0372531 24.786 234 157 8 143 368 64 286 6.66e-13 68.9
Msa1407750 MtrunA17_Chr7g0269701 25.614 285 156 10 119 368 24 287 4.15e-12 66.6
Msa1407750 MtrunA17_Chr6g0488341 23.043 230 143 4 145 369 13 213 2.44e-11 63.2
Msa1407750 MtrunA17_Chr1g0201361 24.324 259 163 7 119 369 53 286 9.73e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1407750 AT5G64970.3 62.857 385 102 4 20 369 26 404 5.23e-156 444
Msa1407750 AT5G64970.1 62.857 385 102 4 20 369 26 404 7.53e-156 445
Msa1407750 AT5G64970.2 75.093 269 50 1 118 369 12 280 3.07e-141 403
Msa1407750 AT1G78180.1 56.021 382 137 3 12 369 23 397 3.64e-124 364
Msa1407750 AT4G32400.1 33.696 276 142 6 115 369 112 367 1.61e-39 145
Msa1407750 AT4G32400.1 30.220 182 104 5 189 369 110 269 1.73e-14 74.7
Msa1407750 AT3G55640.1 29.392 296 152 7 111 369 32 307 3.29e-30 118
Msa1407750 AT3G55640.4 29.392 296 152 7 111 369 32 307 3.29e-30 118
Msa1407750 AT2G37890.1 26.733 303 165 8 104 369 32 314 2.04e-28 113
Msa1407750 AT2G37890.1 25.758 198 119 5 107 278 142 337 4.89e-12 67.0
Msa1407750 AT4G01100.1 30.492 305 150 9 112 369 37 326 6.94e-28 112
Msa1407750 AT4G01100.1 29.054 148 84 3 145 271 195 342 3.06e-13 70.5
Msa1407750 AT3G53940.1 28.094 299 156 7 109 369 65 342 2.22e-27 111
Msa1407750 AT3G53940.1 25.926 189 114 5 107 270 170 357 3.40e-12 67.4
Msa1407750 AT5G01500.1 32.889 225 122 8 145 368 173 369 6.54e-26 108
Msa1407750 AT5G01500.1 25.481 208 134 3 108 298 210 413 4.83e-11 64.3
Msa1407750 AT3G51870.1 28.996 269 134 10 128 368 102 341 1.31e-25 106
Msa1407750 AT3G51870.1 24.000 200 131 4 101 283 175 370 7.61e-11 63.5
Msa1407750 AT5G51050.1 28.378 296 170 8 96 369 202 477 1.43e-25 108
Msa1407750 AT5G51050.1 26.882 186 109 4 111 272 312 494 1.98e-13 71.6
Msa1407750 AT4G01100.3 30.241 291 141 9 126 369 1 276 2.19e-25 104
Msa1407750 AT4G01100.3 29.054 148 84 3 145 271 145 292 2.18e-13 70.5
Msa1407750 AT4G01100.2 28.840 319 151 9 112 369 37 340 6.19e-25 104
Msa1407750 AT4G01100.2 29.054 148 84 3 145 271 209 356 3.73e-13 70.5
Msa1407750 AT3G55640.2 32.308 195 100 4 111 273 32 226 3.68e-24 100
Msa1407750 AT3G55640.3 32.308 195 100 4 111 273 32 226 3.68e-24 100
Msa1407750 AT4G26180.2 26.102 295 156 7 115 369 19 291 1.13e-23 99.8
Msa1407750 AT4G26180.1 26.102 295 156 7 115 369 19 291 2.84e-23 99.4
Msa1407750 AT3G20240.1 25.939 293 158 8 115 369 50 321 2.69e-21 94.0
Msa1407750 AT3G53940.2 30.198 202 108 4 109 277 65 266 7.15e-21 92.0
Msa1407750 AT5G61810.2 25.685 292 178 6 96 369 44 314 4.03e-19 87.8
Msa1407750 AT5G07320.1 27.551 294 170 6 96 369 188 458 1.39e-18 87.4
Msa1407750 AT5G61810.1 25.685 292 178 6 96 369 187 457 1.50e-18 87.4
Msa1407750 AT5G48970.2 25.726 241 153 6 145 368 13 244 1.81e-18 84.7
Msa1407750 AT5G48970.2 26.282 156 91 3 144 276 111 265 6.55e-13 68.6
Msa1407750 AT5G48970.1 23.588 301 170 7 119 368 24 315 2.08e-18 85.9
Msa1407750 AT5G48970.1 26.282 156 91 3 144 276 182 336 1.03e-12 68.9
Msa1407750 AT1G14560.2 28.295 258 127 7 113 334 23 258 4.71e-18 84.3
Msa1407750 AT1G14560.1 28.395 243 116 7 128 334 38 258 6.88e-18 84.0
Msa1407750 AT3G21390.1 22.483 298 176 7 119 368 21 311 1.68e-15 77.0
Msa1407750 AT3G21390.1 28.188 149 83 3 144 268 177 325 4.13e-12 67.0
Msa1407750 AT5G17400.1 29.630 243 129 9 141 367 65 281 1.90e-15 76.6
Msa1407750 AT3G21390.2 22.483 298 176 7 119 368 43 333 2.11e-15 77.0
Msa1407750 AT3G21390.2 28.188 149 83 3 144 268 199 347 4.26e-12 67.0
Msa1407750 AT4G39460.2 24.921 317 193 10 74 369 1 293 3.74e-14 73.2
Msa1407750 AT4G39460.1 24.921 317 193 10 74 369 1 293 3.74e-14 73.2
Msa1407750 AT4G39460.3 24.921 317 193 10 74 369 1 293 3.74e-14 73.2
Msa1407750 AT4G11440.1 24.638 276 165 7 115 367 328 583 8.27e-14 73.2
Msa1407750 AT4G11440.2 24.638 276 165 7 115 367 328 583 8.27e-14 73.2
Msa1407750 AT1G34065.1 24.621 264 168 8 113 369 78 317 1.24e-13 71.6
Msa1407750 AT1G34065.1 27.211 147 105 2 125 270 187 332 4.26e-11 63.9
Msa1407750 AT1G34065.2 24.621 264 168 8 113 369 71 310 1.42e-13 71.6
Msa1407750 AT1G34065.2 27.211 147 105 2 125 270 180 325 4.30e-11 63.9
Msa1407750 AT5G26200.1 26.984 252 135 9 143 364 82 314 1.40e-12 68.6
Msa1407750 AT5G66380.1 24.051 237 154 6 143 368 60 281 8.32e-12 65.9
Msa1407750 AT1G79900.1 28.986 138 91 4 144 277 154 288 1.12e-11 65.5
Msa1407750 AT2G26360.1 32.576 132 86 3 144 274 248 377 3.01e-11 64.7
Msa1407750 AT4G28390.1 23.103 290 158 9 120 368 86 351 4.23e-11 64.3
Msa1407750 AT4G28390.2 23.103 290 158 9 120 368 86 351 4.23e-11 64.3

Find 170 sgRNAs with CRISPR-Local

Find 281 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAAGGTTTGAAAGGATTT+TGG 0.117339 tig0023324:-43367 Msa1407750:intron
TCTTCGGACGGCACCCTTTA+AGG 0.140830 tig0023324:-43324 Msa1407750:CDS
TGTCTCGGTGTTTATTTAAT+TGG 0.173944 tig0023324:+44747 None:intergenic
CTATGGACTTTGGGATTCTT+TGG 0.217085 tig0023324:+41642 None:intergenic
ACCATTAGAACTTTATTATA+TGG 0.235225 tig0023324:-42261 Msa1407750:CDS
AATAAACTAGTCAGAATATC+AGG 0.236453 tig0023324:-43269 Msa1407750:CDS
TGTTCCAACTTTGATAAATT+AGG 0.282644 tig0023324:+41930 None:intergenic
TCTGTTCCAACGTTGTATTT+TGG 0.311110 tig0023324:-41954 Msa1407750:three_prime_UTR
ACAAATCAGAGTTGGGTTCT+TGG 0.319818 tig0023324:+44503 None:intergenic
TGAGTAATCGGCCACCTCTT+AGG 0.320752 tig0023324:+41236 None:intergenic
TGCTTTGGAACAACTGGAAT+TGG 0.323427 tig0023324:-42286 Msa1407750:CDS
TTTGTTGCTGGTGCTGCTGC+AGG 0.327887 tig0023324:-43218 Msa1407750:CDS
TTATTATATGGTGCTATTGC+GGG 0.329863 tig0023324:-42249 Msa1407750:CDS
AAGGAGACAGCTTCAATTGC+AGG 0.333519 tig0023324:-42187 Msa1407750:CDS
TTGAAACAACAGATATAGTT+TGG 0.344457 tig0023324:-41303 Msa1407750:three_prime_UTR
TTTCTGCTCTGAAACTTGAT+TGG 0.353094 tig0023324:+44246 None:intergenic
ATTAACTCCCAGCTTATTGC+AGG 0.354263 tig0023324:-42085 Msa1407750:CDS
CATATCCCTTTGAAGTTGTA+AGG 0.358768 tig0023324:-42206 Msa1407750:CDS
ACAGGAAACAGTGGTAGAAT+TGG 0.363567 tig0023324:+44399 None:intergenic
TGGACCTAATTTATCAAAGT+TGG 0.364548 tig0023324:-41934 Msa1407750:three_prime_UTR
AGAAGAGAAGGAGAATATTC+AGG 0.364643 tig0023324:-44198 Msa1407750:intron
AAATCCATCCTATGGACTTT+GGG 0.365330 tig0023324:+41633 None:intergenic
ATTCCCTTACCGTGGTGAAT+TGG 0.370471 tig0023324:-41851 Msa1407750:three_prime_UTR
GACAGCTTCAATTGCAGGTT+AGG 0.374549 tig0023324:-42182 Msa1407750:CDS
AAGTATATGTTGAAAATAAG+AGG 0.374726 tig0023324:+42057 None:intergenic
GAAGATGTCGTCCAATGATA+TGG 0.376812 tig0023324:+40995 None:intergenic
TTATAAAATGTGTGAGTAAT+CGG 0.377865 tig0023324:+41224 None:intergenic
GGAACAGAAATCGTCTAATC+TGG 0.378469 tig0023324:+41969 None:intergenic
GCTGAATGCTTTGGAACAAC+TGG 0.379168 tig0023324:-42292 Msa1407750:CDS
TAAAATTGTGTTCTTGTGTA+TGG 0.389830 tig0023324:-41805 Msa1407750:three_prime_UTR
CTGGCAATGCTTTGATGAAT+AGG 0.390978 tig0023324:-41157 Msa1407750:three_prime_UTR
CGCCTCTTCATTGTGCTGTA+AGG 0.391075 tig0023324:+44790 None:intergenic
AAAATCCATCCTATGGACTT+TGG 0.393894 tig0023324:+41632 None:intergenic
TTCCCTTACCGTGGTGAATT+GGG 0.394369 tig0023324:-41850 Msa1407750:three_prime_UTR
AGTCAACACTTGGAATTTCA+TGG 0.398124 tig0023324:+41769 None:intergenic
TCCTTGAGTTTGTCTATAAA+CGG 0.400844 tig0023324:-44301 Msa1407750:CDS
GTTGTCGACCAGGTGTGGTT+TGG 0.403258 tig0023324:-41675 Msa1407750:three_prime_UTR
AGCACCTGGCGGTGAAGCAT+TGG 0.405799 tig0023324:-42514 Msa1407750:CDS
CTTAAGGTTCAAGGTGCTGC+TGG 0.408783 tig0023324:-44337 Msa1407750:CDS
TAGTTGGGTTTGTCTGTGTT+TGG 0.410204 tig0023324:+44573 None:intergenic
AACAAATCTCTCAAAATTTG+TGG 0.410653 tig0023324:+43235 None:intergenic
ACCATATAATAAAGTTCTAA+TGG 0.412646 tig0023324:+42260 None:intergenic
AAGGAGAATATTCAGGTTCA+TGG 0.413582 tig0023324:-44191 Msa1407750:intron
TGAAGGTGCCATGCTGATAA+TGG 0.413657 tig0023324:+42416 None:intergenic
TCTGATTTGTTCTTCTTCCC+AGG 0.417191 tig0023324:-44490 Msa1407750:CDS
TTTGAGATTCTTAAGGTTCA+AGG 0.432482 tig0023324:-44346 Msa1407750:CDS
AGGTTATACCTGCAATAAGC+TGG 0.438087 tig0023324:+42077 None:intergenic
TGTTGGAAGAGCCGTTGATC+TGG 0.442023 tig0023324:+44639 None:intergenic
ATACACTGTCTAGGTCTCAT+TGG 0.449140 tig0023324:-41189 Msa1407750:three_prime_UTR
GGGTCAAGAGCTGAATGCTT+TGG 0.450331 tig0023324:-42301 Msa1407750:CDS
TTGTTGCTGGTGCTGCTGCA+GGG 0.450597 tig0023324:-43217 Msa1407750:CDS
ATTGTATAAAATCCATCCTA+TGG 0.450652 tig0023324:+41625 None:intergenic
CGCATTTCATTCCTTTCGAT+CGG 0.454099 tig0023324:-44692 Msa1407750:five_prime_UTR
GCTTTGGAACAACTGGAATT+GGG 0.455395 tig0023324:-42285 Msa1407750:CDS
GCCCCAGTACTTGTCCTATA+TGG 0.455593 tig0023324:+41562 None:intergenic
GCACCTGGCGGTGAAGCATT+GGG 0.459088 tig0023324:-42513 Msa1407750:CDS
CGACCATATAGGACAAGTAC+TGG 0.462450 tig0023324:-41565 Msa1407750:three_prime_UTR
GGTGTTCCTGCTCTCTATGC+AGG 0.463872 tig0023324:-42108 Msa1407750:CDS
GAAGCATTGGGAGGTGTAAT+CGG 0.464975 tig0023324:-42501 Msa1407750:CDS
ATTATCAGCATGGCACCTTC+AGG 0.465225 tig0023324:-42414 Msa1407750:CDS
CCTCCCAATGCTTCACCGCC+AGG 0.467880 tig0023324:+42510 None:intergenic
GTCTCATTGGATTCTACTGC+TGG 0.468406 tig0023324:-41176 Msa1407750:three_prime_UTR
GTAGAATTGGATGAAGAAGA+TGG 0.469567 tig0023324:+44412 None:intergenic
ATGACCAAACACCTCTGGTC+TGG 0.470639 tig0023324:-44152 Msa1407750:CDS
TGTCGTCCAATGATATGGTT+AGG 0.470864 tig0023324:+41000 None:intergenic
AGCACCAGACCAGAGGTGTT+TGG 0.476230 tig0023324:+44148 None:intergenic
GAGAATATTCTCATCTTCAA+TGG 0.481124 tig0023324:+41280 None:intergenic
AACCTTGCTCTGCTTGCCTA+TGG 0.481521 tig0023324:-43186 Msa1407750:CDS
TTCTTCATCTTCGACCATAT+AGG 0.482500 tig0023324:-41576 Msa1407750:three_prime_UTR
TAGGACAAGTACTGGGGCCC+TGG 0.488382 tig0023324:-41557 Msa1407750:intron
TGTCTCCTTACAACTTCAAA+GGG 0.489648 tig0023324:+42201 None:intergenic
GCAACTTCTCAAGGTTTGAA+AGG 0.494025 tig0023324:-43374 Msa1407750:intron
TTTATTATATGGTGCTATTG+CGG 0.495204 tig0023324:-42250 Msa1407750:CDS
AATATTCAGGTTCATGGATC+GGG 0.498629 tig0023324:-44185 Msa1407750:CDS
CTTTCAGATCAGGACAGTAA+TGG 0.504931 tig0023324:-42538 Msa1407750:intron
AGAGATAATTTGTTAGCAGT+AGG 0.506311 tig0023324:-41059 Msa1407750:three_prime_UTR
GAAGAAGAACAAATCAGAGT+TGG 0.508478 tig0023324:+44495 None:intergenic
AAGAAGAACAAATCAGAGTT+GGG 0.508819 tig0023324:+44496 None:intergenic
AGAAGGACGAGCCGATCGAA+AGG 0.510509 tig0023324:+44681 None:intergenic
TGGGATGCTGCAGGTAATGA+AGG 0.516343 tig0023324:+44432 None:intergenic
CTGTCTCCTTACAACTTCAA+AGG 0.518936 tig0023324:+42200 None:intergenic
GATCCCAATTCACCACGGTA+AGG 0.519037 tig0023324:+41847 None:intergenic
TAGTGTCCTAACCATATCAT+TGG 0.522874 tig0023324:-41006 Msa1407750:three_prime_UTR
ATATCATTGGACGACATCTT+CGG 0.524723 tig0023324:-40993 None:intergenic
TCTAATCTGGATCATGGCTC+AGG 0.525610 tig0023324:+41982 None:intergenic
CAAATAATCCAAACCACACC+TGG 0.528370 tig0023324:+41667 None:intergenic
TAGGAACAGTGTTGGCATGA+AGG 0.528503 tig0023324:+44458 None:intergenic
ACAGCTTCAATTGCAGGTTA+GGG 0.529291 tig0023324:-42181 Msa1407750:CDS
TGTTGCTGGTGCTGCTGCAG+GGG 0.530011 tig0023324:-43216 Msa1407750:CDS
GACCATATAGGACAAGTACT+GGG 0.530845 tig0023324:-41564 Msa1407750:three_prime_UTR
TCAACATGACCAAACACCTC+TGG 0.535107 tig0023324:-44157 Msa1407750:CDS
TTTGGTACGAAAAGTTGATC+TGG 0.535674 tig0023324:+44591 None:intergenic
GCTGTAAGGTCTGCTGTTGG+TGG 0.537303 tig0023324:+44804 None:intergenic
AAATCGTCTAATCTGGATCA+TGG 0.543522 tig0023324:+41976 None:intergenic
GTCAACACTTGGAATTTCAT+GGG 0.544807 tig0023324:+41770 None:intergenic
GAATATTCAGGTTCATGGAT+CGG 0.545360 tig0023324:-44186 Msa1407750:CDS
TTGCATAGATAGTCAACACT+TGG 0.546500 tig0023324:+41759 None:intergenic
ATCCCAATTCACCACGGTAA+GGG 0.552089 tig0023324:+41848 None:intergenic
GACCTTACAGCACAATGAAG+AGG 0.556668 tig0023324:-44792 Msa1407750:five_prime_UTR
GTTAATCCTGCATAGAGAGC+AGG 0.556840 tig0023324:+42102 None:intergenic
TTTGACAACATACACTGTCT+AGG 0.558995 tig0023324:-41198 Msa1407750:three_prime_UTR
TGGAATATCAGGAAAAGTGT+CGG 0.562118 tig0023324:-44370 Msa1407750:CDS
TCGGCCACCTCTTAGGTCCA+TGG 0.563547 tig0023324:+41243 None:intergenic
GCATGAAGGAAAAGACCACC+TGG 0.565089 tig0023324:+44472 None:intergenic
CAAAGAATCCCAAAGTCCAT+AGG 0.565211 tig0023324:-41641 Msa1407750:three_prime_UTR
CGCCATATGATCAAAACTGA+GGG 0.565763 tig0023324:-42471 Msa1407750:CDS
ACCACTTGAGAGACTTAAGC+TGG 0.566075 tig0023324:-43450 Msa1407750:CDS
TCTTGCAAGAAAACAGAGGA+GGG 0.566901 tig0023324:-41720 Msa1407750:three_prime_UTR
AAGCTGGAATACATAGTTCG+TGG 0.567190 tig0023324:-43434 Msa1407750:intron
AGTAGTATGCAGGATGTCCA+AGG 0.568282 tig0023324:+41028 None:intergenic
GAATCCCAAAGTCCATAGGA+TGG 0.571710 tig0023324:-41637 Msa1407750:three_prime_UTR
CCTTCAGGTGCTGTCTACTA+CGG 0.579921 tig0023324:-42399 Msa1407750:CDS
TAGAATTGGATGAAGAAGAT+GGG 0.581434 tig0023324:+44413 None:intergenic
GTCCATAGGCAAGCAGAGCA+AGG 0.582869 tig0023324:+43184 None:intergenic
TTAGGACACTAGTAGTATGC+AGG 0.583138 tig0023324:+41018 None:intergenic
CTTCAATATATGAAAGAAGA+GGG 0.583776 tig0023324:-42321 Msa1407750:CDS
TGTGCTGTAAGGTCTGCTGT+TGG 0.584129 tig0023324:+44801 None:intergenic
TTTGCACTCACAGTGTCCAT+AGG 0.584166 tig0023324:+43170 None:intergenic
ATCGTGCAAGTTTGATATGA+AGG 0.585200 tig0023324:-41904 Msa1407750:three_prime_UTR
GTCGAGTTGTCGACCAGGTG+TGG 0.585815 tig0023324:-41680 Msa1407750:three_prime_UTR
AGCAGTAGGAATCTAACCCT+TGG 0.586003 tig0023324:-41045 Msa1407750:three_prime_UTR
GCATATCTGCATTCACCCGA+GGG 0.587818 tig0023324:-42354 Msa1407750:CDS
TCCGTTTATAGACAAACTCA+AGG 0.588085 tig0023324:+44300 None:intergenic
TTCTTAATAAGCCAGATCAA+CGG 0.588771 tig0023324:-44650 Msa1407750:five_prime_UTR
GGTTATACCTGCAATAAGCT+GGG 0.590199 tig0023324:+42078 None:intergenic
AATTTATCAAAGTTGGAACA+TGG 0.590205 tig0023324:-41927 Msa1407750:three_prime_UTR
ACTTCAATATATGAAAGAAG+AGG 0.591477 tig0023324:-42322 Msa1407750:CDS
TGAAGAAGACCTTGAGACCA+TGG 0.593221 tig0023324:+44118 None:intergenic
TTCTTGCAAGAAAACAGAGG+AGG 0.595488 tig0023324:-41721 Msa1407750:three_prime_UTR
GCAATGCTTTGATGAATAGG+AGG 0.599931 tig0023324:-41154 Msa1407750:three_prime_UTR
GATTTGTTCTTCTTCCCAGG+TGG 0.601976 tig0023324:-44487 Msa1407750:CDS
AATGAGAGAGATAAATGTCT+CGG 0.608008 tig0023324:+44732 None:intergenic
ATAAACTAGTCAGAATATCA+GGG 0.609171 tig0023324:-43268 Msa1407750:CDS
GAAGGAGGTAGGAACAGTGT+TGG 0.612519 tig0023324:+44450 None:intergenic
AATTGTGTTCTTGTGTATGG+AGG 0.613315 tig0023324:-41802 Msa1407750:three_prime_UTR
TGAAGAAGATGGGATGCTGC+AGG 0.613995 tig0023324:+44423 None:intergenic
AAGGTTCAAGGTGCTGCTGG+TGG 0.615600 tig0023324:-44334 Msa1407750:CDS
GTCCACACCATGGACCTAAG+AGG 0.616241 tig0023324:-41250 Msa1407750:three_prime_UTR
TGTGTCAAGATTGTTGAGCA+AGG 0.616757 tig0023324:-42132 Msa1407750:CDS
AGCATATCTGCATTCACCCG+AGG 0.623364 tig0023324:-42355 Msa1407750:CDS
AAGTAACTAATAGCAGCAGA+TGG 0.625598 tig0023324:+41392 None:intergenic
GTCCATGGTGTGGACACAAG+TGG 0.626040 tig0023324:+41258 None:intergenic
CTGCAGGTAATGAAGGAGGT+AGG 0.627394 tig0023324:+44439 None:intergenic
TATATGTCGAGTTGTCGACC+AGG 0.633214 tig0023324:-41685 Msa1407750:three_prime_UTR
AGTACCCTCCATTATCAGCA+TGG 0.633664 tig0023324:-42424 Msa1407750:CDS
CTCCACTTGTGTCCACACCA+TGG 0.634128 tig0023324:-41260 Msa1407750:three_prime_UTR
CCGCCATATGATCAAAACTG+AGG 0.638358 tig0023324:-42472 Msa1407750:CDS
TCCAGCTTAAGTCTCTCAAG+TGG 0.638459 tig0023324:+43449 None:intergenic
AGGACAGTAATGGTAGCACC+TGG 0.645057 tig0023324:-42528 Msa1407750:CDS
CGGCAAAAGCACCAGACCAG+AGG 0.651558 tig0023324:+44141 None:intergenic
CCGTAGTAGACAGCACCTGA+AGG 0.655494 tig0023324:+42399 None:intergenic
ACAGCACAATGAAGAGGCGA+CGG 0.657096 tig0023324:-44786 Msa1407750:five_prime_UTR
AGGTGCCATGCTGATAATGG+AGG 0.661611 tig0023324:+42419 None:intergenic
GCACAATGAAGAGGCGACGG+TGG 0.662699 tig0023324:-44783 Msa1407750:five_prime_UTR
TTAGGTCCAAAATACAACGT+TGG 0.669579 tig0023324:+41948 None:intergenic
GTAGTATGCAGGATGTCCAA+GGG 0.669681 tig0023324:+41029 None:intergenic
CACCTCTTAGGTCCATGGTG+TGG 0.679141 tig0023324:+41248 None:intergenic
ATCTGGATCATGGCTCAGGT+AGG 0.680333 tig0023324:+41986 None:intergenic
ACCATATAGGACAAGTACTG+GGG 0.682727 tig0023324:-41563 Msa1407750:three_prime_UTR
TGCAAGAAAACAGAGGAGGG+AGG 0.683705 tig0023324:-41717 Msa1407750:three_prime_UTR
CCTGGCGGTGAAGCATTGGG+AGG 0.688081 tig0023324:-42510 Msa1407750:CDS
CACACCATGGACCTAAGAGG+TGG 0.690827 tig0023324:-41247 Msa1407750:three_prime_UTR
GATGCTGCAGGTAATGAAGG+AGG 0.698778 tig0023324:+44435 None:intergenic
CATGAAGGAAAAGACCACCT+GGG 0.702840 tig0023324:+44473 None:intergenic
GCATGGATCCCAATTCACCA+CGG 0.721686 tig0023324:+41842 None:intergenic
GGTGCCATGCTGATAATGGA+GGG 0.735104 tig0023324:+42420 None:intergenic
GTCAAGATTGTTGAGCAAGG+AGG 0.739447 tig0023324:-42129 Msa1407750:CDS
ACAGTAATGGTAGCACCTGG+CGG 0.741224 tig0023324:-42525 Msa1407750:CDS
AATGTGTGATTCCCTTACCG+TGG 0.773955 tig0023324:-41859 Msa1407750:three_prime_UTR
AGAAGACCTTGAGACCATGG+CGG 0.787349 tig0023324:+44121 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTATATGTATCAT+AGG + tig0023324:42520-42539 None:intergenic 10.0%
!! TAGAAAAAGAAAAAAAAAAT+GGG + tig0023324:42877-42896 None:intergenic 10.0%
!! TTAGAAAAAGAAAAAAAAAA+TGG + tig0023324:42878-42897 None:intergenic 10.0%
!!! TTTTTTTCTTTTTCTAATTT+CGG - tig0023324:42881-42900 Msa1407750:intron 10.0%
!! AAAAATTGTAGTTATGATAT+GGG + tig0023324:41859-41878 None:intergenic 15.0%
!! AAAAAATTGTAGTTATGATA+TGG + tig0023324:41860-41879 None:intergenic 15.0%
!!! TATCATAACTACAATTTTTT+AGG - tig0023324:41860-41879 Msa1407750:three_prime_UTR 15.0%
!!! TAAAAATTTATAGCCTTAAA+GGG + tig0023324:42495-42514 None:intergenic 15.0%
!!! ATAAAAATTTATAGCCTTAA+AGG + tig0023324:42496-42515 None:intergenic 15.0%
!! TAAATGATATGAATTCTATA+AGG + tig0023324:44279-44298 None:intergenic 15.0%
!! TATAGAATTCATATCATTTA+AGG - tig0023324:44279-44298 Msa1407750:CDS 15.0%
!!! ATTTTATTTATAGTACTGAG+TGG - tig0023324:41833-41852 Msa1407750:three_prime_UTR 20.0%
!!! ACTACAATTTTTTAGGATAA+AGG - tig0023324:41867-41886 Msa1407750:three_prime_UTR 20.0%
!!! CTACAATTTTTTAGGATAAA+GGG - tig0023324:41868-41887 Msa1407750:three_prime_UTR 20.0%
!! TAATATGAATCAAAGTAAAC+TGG + tig0023324:42136-42155 None:intergenic 20.0%
!! AGTTTACTTTGATTCATATT+AGG - tig0023324:42135-42154 Msa1407750:CDS 20.0%
!!! GTTTTTTAAAAAGAAAAGAC+AGG + tig0023324:42206-42225 None:intergenic 20.0%
!! GTGTATTGTATTAATTATGT+TGG - tig0023324:42310-42329 Msa1407750:CDS 20.0%
!!! ATTAATTATGTTGGTTTTTC+AGG - tig0023324:42319-42338 Msa1407750:CDS 20.0%
!!! GAAATTTTGTGAATATTCTT+CGG - tig0023324:42463-42482 Msa1407750:CDS 20.0%
!! ATATGTGTTTAAATGTTGTT+TGG - tig0023324:42695-42714 Msa1407750:intron 20.0%
!! TTTGAAAATTGAAAGTCATT+GGG + tig0023324:42827-42846 None:intergenic 20.0%
!!! TTTTGAAAATTGAAAGTCAT+TGG + tig0023324:42828-42847 None:intergenic 20.0%
!!! ACTTTCAATTTTCAAAATGT+TGG - tig0023324:42831-42850 Msa1407750:intron 20.0%
!! AAACTATATGATAAGTTTAC+TGG - tig0023324:42976-42995 Msa1407750:intron 20.0%
!! AACTATATGATAAGTTTACT+GGG - tig0023324:42977-42996 Msa1407750:intron 20.0%
!! ACATGATGTTAATTATTTAG+TGG - tig0023324:43041-43060 Msa1407750:intron 20.0%
!! AAACTCCTTTAATAATTTAG+TGG - tig0023324:43106-43125 Msa1407750:intron 20.0%
!! ACCATTAGAACTTTATTATA+TGG - tig0023324:43542-43561 Msa1407750:intron 20.0%
!!! ACCATATAATAAAGTTCTAA+TGG + tig0023324:43546-43565 None:intergenic 20.0%
!! AAGTATATGTTGAAAATAAG+AGG + tig0023324:43749-43768 None:intergenic 20.0%
!! TTATAAAATGTGTGAGTAAT+CGG + tig0023324:44582-44601 None:intergenic 20.0%
!!! TTTTTATAGACAACTATTAG+CGG - tig0023324:44706-44725 Msa1407750:five_prime_UTR 20.0%
! TATTAAGAAGAAGAAGAAGA+AGG + tig0023324:41142-41161 None:intergenic 25.0%
!!! TTTTTTTTTTCACACGTTGT+TGG + tig0023324:41184-41203 None:intergenic 25.0%
!!! TTTTGTTTTTTGCTGTAGTT+GGG + tig0023324:41248-41267 None:intergenic 25.0%
!!! TTTTTGTTTTTTGCTGTAGT+TGG + tig0023324:41249-41268 None:intergenic 25.0%
! GATACTTATATGAGAATCAA+TGG + tig0023324:41798-41817 None:intergenic 25.0%
!!! TACCAAAGATTTTTTCAACT+AGG - tig0023324:41933-41952 Msa1407750:three_prime_UTR 25.0%
!!! ACCAAAGATTTTTTCAACTA+GGG - tig0023324:41934-41953 Msa1407750:three_prime_UTR 25.0%
! ATCATAATAGTGCATATGAA+TGG + tig0023324:42277-42296 None:intergenic 25.0%
! ATGCACTATTATGATAAAGA+TGG - tig0023324:42282-42301 Msa1407750:CDS 25.0%
! TATTATGATAAAGATGGTGA+CGG - tig0023324:42288-42307 Msa1407750:CDS 25.0%
! AATAAACTAGTCAGAATATC+AGG - tig0023324:42534-42553 Msa1407750:CDS 25.0%
! ATAAACTAGTCAGAATATCA+GGG - tig0023324:42535-42554 Msa1407750:intron 25.0%
! AACAAATCTCTCAAAATTTG+TGG + tig0023324:42571-42590 None:intergenic 25.0%
! AAAATAATATCAGAGCTAAG+AGG + tig0023324:42667-42686 None:intergenic 25.0%
! TTTGACAAGTATGATATGAT+TGG - tig0023324:42728-42747 Msa1407750:intron 25.0%
! AGATTAGGAAATAAGAAAAG+AGG + tig0023324:42952-42971 None:intergenic 25.0%
!!! TCATATAGTTTTCTGAGATT+AGG + tig0023324:42967-42986 None:intergenic 25.0%
! TCATGTGAAAAAGAAATAGT+TGG + tig0023324:43027-43046 None:intergenic 25.0%
! AGGAACCACTAAATTATTAA+AGG + tig0023324:43114-43133 None:intergenic 25.0%
! AAACTAACATATCCACTAAA+TGG + tig0023324:43172-43191 None:intergenic 25.0%
! AAACACTAGTATATTTCAGT+AGG + tig0023324:43202-43221 None:intergenic 25.0%
! ACTTCAATATATGAAAGAAG+AGG - tig0023324:43481-43500 Msa1407750:intron 25.0%
! CTTCAATATATGAAAGAAGA+GGG - tig0023324:43482-43501 Msa1407750:intron 25.0%
!!! TTTATTATATGGTGCTATTG+CGG - tig0023324:43553-43572 Msa1407750:intron 25.0%
! TGTTCCAACTTTGATAAATT+AGG + tig0023324:43876-43895 None:intergenic 25.0%
! AATTTATCAAAGTTGGAACA+TGG - tig0023324:43876-43895 Msa1407750:intron 25.0%
! TAAAATTGTGTTCTTGTGTA+TGG - tig0023324:43998-44017 Msa1407750:intron 25.0%
! ATTGTATAAAATCCATCCTA+TGG + tig0023324:44181-44200 None:intergenic 25.0%
! GATCAGACAATTATAATTGA+CGG + tig0023324:44341-44360 None:intergenic 25.0%
! TTGAAACAACAGATATAGTT+TGG - tig0023324:44500-44519 Msa1407750:CDS 25.0%
!! TGTCTCGGTGTTTATTTAAT+TGG + tig0023324:41059-41078 None:intergenic 30.0%
AATGAGAGAGATAAATGTCT+CGG + tig0023324:41074-41093 None:intergenic 30.0%
TTCTTAATAAGCCAGATCAA+CGG - tig0023324:41153-41172 Msa1407750:three_prime_UTR 30.0%
AAGAAGAACAAATCAGAGTT+GGG + tig0023324:41310-41329 None:intergenic 30.0%
TAGAATTGGATGAAGAAGAT+GGG + tig0023324:41393-41412 None:intergenic 30.0%
! TTTGAGATTCTTAAGGTTCA+AGG - tig0023324:41457-41476 Msa1407750:intron 30.0%
TCCTTGAGTTTGTCTATAAA+CGG - tig0023324:41502-41521 Msa1407750:intron 30.0%
GTCTCAATTACAGATTATCA+TGG + tig0023324:41895-41914 None:intergenic 30.0%
TCCCTAGTTGAAAAAATCTT+TGG + tig0023324:41938-41957 None:intergenic 30.0%
TCACTGAGAATTTCAAAAGT+TGG - tig0023324:42046-42065 Msa1407750:three_prime_UTR 30.0%
CACTGAGAATTTCAAAAGTT+GGG - tig0023324:42047-42066 Msa1407750:three_prime_UTR 30.0%
!! GAAGCTTTAATGTACAGTTT+TGG - tig0023324:42177-42196 Msa1407750:CDS 30.0%
TGCATTTCATTTATTTCACC+CGG + tig0023324:42244-42263 None:intergenic 30.0%
! TTTTGTGAATATTCTTCGGA+CGG - tig0023324:42467-42486 Msa1407750:CDS 30.0%
!!! AATTTTGAGAGATTTGTTGC+TGG - tig0023324:42573-42592 Msa1407750:intron 30.0%
! TGTTGTTTGTAGACTGATTT+GGG - tig0023324:42781-42800 Msa1407750:intron 30.0%
! TCAAACCATTGGTTAAACTA+AGG + tig0023324:43134-43153 None:intergenic 30.0%
TAACATATCCACTAAATGGT+AGG + tig0023324:43168-43187 None:intergenic 30.0%
!! TTATTATATGGTGCTATTGC+GGG - tig0023324:43554-43573 Msa1407750:intron 30.0%
TGGACCTAATTTATCAAAGT+TGG - tig0023324:43869-43888 Msa1407750:intron 30.0%
!! TTACACTGCTTTTATTTGCA+TGG + tig0023324:43981-44000 None:intergenic 30.0%
! GACGGTGTAAAACTTTTTAA+CGG + tig0023324:44323-44342 None:intergenic 30.0%
GAGAATATTCTCATCTTCAA+TGG + tig0023324:44526-44545 None:intergenic 30.0%
!! AGAGATAATTTGTTAGCAGT+AGG - tig0023324:44744-44763 Msa1407750:five_prime_UTR 30.0%
! TTTGGTACGAAAAGTTGATC+TGG + tig0023324:41215-41234 None:intergenic 35.0%
!!! TTGGTTTTGTTTGGACAAGA+GGG + tig0023324:41284-41303 None:intergenic 35.0%
GAAGAAGAACAAATCAGAGT+TGG + tig0023324:41311-41330 None:intergenic 35.0%
GTAGAATTGGATGAAGAAGA+TGG + tig0023324:41394-41413 None:intergenic 35.0%
CCTGATATTCCAAGAAAAAC+AGG + tig0023324:41425-41444 None:intergenic 35.0%
!! CCTGTTTTTCTTGGAATATC+AGG - tig0023324:41422-41441 Msa1407750:three_prime_UTR 35.0%
! TGGAATATCAGGAAAAGTGT+CGG - tig0023324:41433-41452 Msa1407750:three_prime_UTR 35.0%
!!! TGTCGGTTTTGAGATTCTTA+AGG - tig0023324:41450-41469 Msa1407750:three_prime_UTR 35.0%
TCCGTTTATAGACAAACTCA+AGG + tig0023324:41506-41525 None:intergenic 35.0%
!! TTTCTGCTCTGAAACTTGAT+TGG + tig0023324:41560-41579 None:intergenic 35.0%
AGAAGAAGAAGAAGAAGAGA+AGG - tig0023324:41593-41612 Msa1407750:three_prime_UTR 35.0%
AGAAGAGAAGGAGAATATTC+AGG - tig0023324:41605-41624 Msa1407750:three_prime_UTR 35.0%
! AAGGAGAATATTCAGGTTCA+TGG - tig0023324:41612-41631 Msa1407750:three_prime_UTR 35.0%
! GAATATTCAGGTTCATGGAT+CGG - tig0023324:41617-41636 Msa1407750:three_prime_UTR 35.0%
! AATATTCAGGTTCATGGATC+GGG - tig0023324:41618-41637 Msa1407750:three_prime_UTR 35.0%
TGGTGTGACAAAATATAGCA+GGG + tig0023324:41778-41797 None:intergenic 35.0%
ATGGTGTGACAAAATATAGC+AGG + tig0023324:41779-41798 None:intergenic 35.0%
AGAATCACATGTATCCAGTT+CGG + tig0023324:41961-41980 None:intergenic 35.0%
TGAAATTCTCAGTGACCAAA+TGG + tig0023324:42041-42060 None:intergenic 35.0%
! CTCAAGGTTTGAAAGGATTT+TGG - tig0023324:42436-42455 Msa1407750:CDS 35.0%
!! GGTTTGAAAGGATTTTGGAA+AGG - tig0023324:42441-42460 Msa1407750:CDS 35.0%
GATATGATTGGTATGAGTCA+AGG - tig0023324:42740-42759 Msa1407750:intron 35.0%
AAACAACACCACAATGCATA+AGG + tig0023324:42769-42788 None:intergenic 35.0%
!! GTGTTGTTTGTAGACTGATT+TGG - tig0023324:42780-42799 Msa1407750:intron 35.0%
AAAATTGAAAGTCATTGGGC+AGG + tig0023324:42823-42842 None:intergenic 35.0%
TCGGTACTTTATGTTAACTC+TGG - tig0023324:42900-42919 Msa1407750:intron 35.0%
! TAGTGGTTATGTTAGTTTCC+AGG - tig0023324:43058-43077 Msa1407750:intron 35.0%
! AGTGGTTATGTTAGTTTCCA+GGG - tig0023324:43059-43078 Msa1407750:intron 35.0%
TGGTTCCTTAGTTTAACCAA+TGG - tig0023324:43126-43145 Msa1407750:intron 35.0%
AGAGCAATTTCTTTCAGATC+AGG - tig0023324:43255-43274 Msa1407750:CDS 35.0%
! GGCTTCTTTTCTCTTTACAA+AGG - tig0023324:43353-43372 Msa1407750:CDS 35.0%
! TTGAAGTCTTTTCATTCCCT+CGG + tig0023324:43468-43487 None:intergenic 35.0%
!! CATATCCCTTTGAAGTTGTA+AGG - tig0023324:43597-43616 Msa1407750:intron 35.0%
TGTCTCCTTACAACTTCAAA+GGG + tig0023324:43605-43624 None:intergenic 35.0%
AAATCGTCTAATCTGGATCA+TGG + tig0023324:43830-43849 None:intergenic 35.0%
! TCTGTTCCAACGTTGTATTT+TGG - tig0023324:43849-43868 Msa1407750:intron 35.0%
TTAGGTCCAAAATACAACGT+TGG + tig0023324:43858-43877 None:intergenic 35.0%
ATCGTGCAAGTTTGATATGA+AGG - tig0023324:43899-43918 Msa1407750:intron 35.0%
AATTGTGTTCTTGTGTATGG+AGG - tig0023324:44001-44020 Msa1407750:intron 35.0%
GTCAACACTTGGAATTTCAT+GGG + tig0023324:44036-44055 None:intergenic 35.0%
AGTCAACACTTGGAATTTCA+TGG + tig0023324:44037-44056 None:intergenic 35.0%
TTGCATAGATAGTCAACACT+TGG + tig0023324:44047-44066 None:intergenic 35.0%
! CTCTGTTTTCTTGCAAGAAA+AGG + tig0023324:44081-44100 None:intergenic 35.0%
! CTTTTCTTGCAAGAAAACAG+AGG - tig0023324:44079-44098 Msa1407750:intron 35.0%
AAATCCATCCTATGGACTTT+GGG + tig0023324:44173-44192 None:intergenic 35.0%
AAAATCCATCCTATGGACTT+TGG + tig0023324:44174-44193 None:intergenic 35.0%
TTCTTCATCTTCGACCATAT+AGG - tig0023324:44227-44246 Msa1407750:intron 35.0%
TTCTATAAGGATTGCTACCA+GGG + tig0023324:44266-44285 None:intergenic 35.0%
ATTCTATAAGGATTGCTACC+AGG + tig0023324:44267-44286 None:intergenic 35.0%
!! CGTAACTGCTTCTATTTTTC+AGG - tig0023324:44383-44402 Msa1407750:CDS 35.0%
AAGTAACTAATAGCAGCAGA+TGG + tig0023324:44414-44433 None:intergenic 35.0%
TTTGACAACATACACTGTCT+AGG - tig0023324:44605-44624 Msa1407750:five_prime_UTR 35.0%
TAGTGTCCTAACCATATCAT+TGG - tig0023324:44797-44816 Msa1407750:five_prime_UTR 35.0%
CGCATTTCATTCCTTTCGAT+CGG - tig0023324:41111-41130 Msa1407750:three_prime_UTR 40.0%
TAGTTGGGTTTGTCTGTGTT+TGG + tig0023324:41233-41252 None:intergenic 40.0%
!!! CTTGGTTTTGTTTGGACAAG+AGG + tig0023324:41285-41304 None:intergenic 40.0%
!!! GTTGGGTTCTTGGTTTTGTT+TGG + tig0023324:41293-41312 None:intergenic 40.0%
ACAAATCAGAGTTGGGTTCT+TGG + tig0023324:41303-41322 None:intergenic 40.0%
!! TCTGATTTGTTCTTCTTCCC+AGG - tig0023324:41313-41332 Msa1407750:three_prime_UTR 40.0%
ACAGGAAACAGTGGTAGAAT+TGG + tig0023324:41407-41426 None:intergenic 40.0%
CCAAGAAAAACAGGAAACAG+TGG + tig0023324:41416-41435 None:intergenic 40.0%
!! CCACTGTTTCCTGTTTTTCT+TGG - tig0023324:41413-41432 Msa1407750:three_prime_UTR 40.0%
TCAAGTTTCAGAGCAGAAAG+TGG - tig0023324:41561-41580 Msa1407750:three_prime_UTR 40.0%
TAGCAGGGTAGAACAAATTG+TGG + tig0023324:41763-41782 None:intergenic 40.0%
ACGATTTGACTCCAACCATT+TGG - tig0023324:42023-42042 Msa1407750:three_prime_UTR 40.0%
ATTCTCAGTGACCAAATGGT+TGG + tig0023324:42037-42056 None:intergenic 40.0%
AAACGTTTCCAATCAGAAGC+CGG - tig0023324:42222-42241 Msa1407750:CDS 40.0%
AAGCTGGAATACATAGTTCG+TGG - tig0023324:42369-42388 Msa1407750:CDS 40.0%
CAGACAATTGCAACTTCTCA+AGG - tig0023324:42420-42439 Msa1407750:CDS 40.0%
GCAACTTCTCAAGGTTTGAA+AGG - tig0023324:42429-42448 Msa1407750:CDS 40.0%
AATGTCAACGATTCAAACCC+TGG + tig0023324:43079-43098 None:intergenic 40.0%
AGGTGCGTTATTCAAACCAT+TGG + tig0023324:43145-43164 None:intergenic 40.0%
CATATCCACTAAATGGTAGG+AGG + tig0023324:43165-43184 None:intergenic 40.0%
CTTTCAGATCAGGACAGTAA+TGG - tig0023324:43265-43284 Msa1407750:CDS 40.0%
!! CCTCAGTTTTGATCATATGG+CGG + tig0023324:43334-43353 None:intergenic 40.0%
CGCCATATGATCAAAACTGA+GGG - tig0023324:43332-43351 Msa1407750:CDS 40.0%
!! AGCCCTCAGTTTTGATCATA+TGG + tig0023324:43337-43356 None:intergenic 40.0%
! TGAAGTCTTTTCATTCCCTC+GGG + tig0023324:43467-43486 None:intergenic 40.0%
!! TGCTTTGGAACAACTGGAAT+TGG - tig0023324:43517-43536 Msa1407750:intron 40.0%
!! GCTTTGGAACAACTGGAATT+GGG - tig0023324:43518-43537 Msa1407750:intron 40.0%
CTGTCTCCTTACAACTTCAA+AGG + tig0023324:43606-43625 None:intergenic 40.0%
ACAGCTTCAATTGCAGGTTA+GGG - tig0023324:43622-43641 Msa1407750:intron 40.0%
TGTGTCAAGATTGTTGAGCA+AGG - tig0023324:43671-43690 Msa1407750:intron 40.0%
ATTAACTCCCAGCTTATTGC+AGG - tig0023324:43718-43737 Msa1407750:intron 40.0%
GGTTATACCTGCAATAAGCT+GGG + tig0023324:43728-43747 None:intergenic 40.0%
AGGTTATACCTGCAATAAGC+TGG + tig0023324:43729-43748 None:intergenic 40.0%
GGAACAGAAATCGTCTAATC+TGG + tig0023324:43837-43856 None:intergenic 40.0%
TTCTTGCAAGAAAACAGAGG+AGG - tig0023324:44082-44101 Msa1407750:intron 40.0%
TCTTGCAAGAAAACAGAGGA+GGG - tig0023324:44083-44102 Msa1407750:intron 40.0%
CAAATAATCCAAACCACACC+TGG + tig0023324:44139-44158 None:intergenic 40.0%
!! CTATGGACTTTGGGATTCTT+TGG + tig0023324:44164-44183 None:intergenic 40.0%
CAAAGAATCCCAAAGTCCAT+AGG - tig0023324:44162-44181 Msa1407750:CDS 40.0%
GACCATATAGGACAAGTACT+GGG - tig0023324:44239-44258 Msa1407750:intron 40.0%
! ACCATATAGGACAAGTACTG+GGG - tig0023324:44240-44259 Msa1407750:intron 40.0%
ATACACTGTCTAGGTCTCAT+TGG - tig0023324:44614-44633 Msa1407750:five_prime_UTR 40.0%
CTGGCAATGCTTTGATGAAT+AGG - tig0023324:44646-44665 Msa1407750:five_prime_UTR 40.0%
GCAATGCTTTGATGAATAGG+AGG - tig0023324:44649-44668 Msa1407750:five_prime_UTR 40.0%
TTAGGACACTAGTAGTATGC+AGG + tig0023324:44788-44807 None:intergenic 40.0%
TGTCGTCCAATGATATGGTT+AGG + tig0023324:44806-44825 None:intergenic 40.0%
GAAGATGTCGTCCAATGATA+TGG + tig0023324:44811-44830 None:intergenic 40.0%
GACCTTACAGCACAATGAAG+AGG - tig0023324:41011-41030 Msa1407750:three_prime_UTR 45.0%
! GATTTGTTCTTCTTCCCAGG+TGG - tig0023324:41316-41335 Msa1407750:three_prime_UTR 45.0%
CATGAAGGAAAAGACCACCT+GGG + tig0023324:41333-41352 None:intergenic 45.0%
!! TAGGAACAGTGTTGGCATGA+AGG + tig0023324:41348-41367 None:intergenic 45.0%
TCAACATGACCAAACACCTC+TGG - tig0023324:41646-41665 Msa1407750:three_prime_UTR 45.0%
TGAAGAAGACCTTGAGACCA+TGG + tig0023324:41688-41707 None:intergenic 45.0%
! TTTTCAACTAGGGACCGAAC+TGG - tig0023324:41944-41963 Msa1407750:three_prime_UTR 45.0%
AACGTTTCCAATCAGAAGCC+GGG - tig0023324:42223-42242 Msa1407750:CDS 45.0%
TATTTCACCCGGCTTCTGAT+TGG + tig0023324:42233-42252 None:intergenic 45.0%
ACCACTTGAGAGACTTAAGC+TGG - tig0023324:42353-42372 Msa1407750:CDS 45.0%
TCCAGCTTAAGTCTCTCAAG+TGG + tig0023324:42357-42376 None:intergenic 45.0%
TTTGCACTCACAGTGTCCAT+AGG + tig0023324:42636-42655 None:intergenic 45.0%
CAAGGTTGCCTTATGCATTG+TGG - tig0023324:42758-42777 Msa1407750:intron 45.0%
GATTTGGGCAAACGTCACAT+AGG - tig0023324:42796-42815 Msa1407750:intron 45.0%
!! GAAGCATTGGGAGGTGTAAT+CGG - tig0023324:43302-43321 Msa1407750:CDS 45.0%
CCGCCATATGATCAAAACTG+AGG - tig0023324:43331-43350 Msa1407750:CDS 45.0%
AGTACCCTCCATTATCAGCA+TGG - tig0023324:43379-43398 Msa1407750:intron 45.0%
!! TGAAGGTGCCATGCTGATAA+TGG + tig0023324:43390-43409 None:intergenic 45.0%
ATTATCAGCATGGCACCTTC+AGG - tig0023324:43389-43408 Msa1407750:intron 45.0%
!! GCTGAATGCTTTGGAACAAC+TGG - tig0023324:43511-43530 Msa1407750:intron 45.0%
AAGGAGACAGCTTCAATTGC+AGG - tig0023324:43616-43635 Msa1407750:intron 45.0%
GACAGCTTCAATTGCAGGTT+AGG - tig0023324:43621-43640 Msa1407750:intron 45.0%
GTCAAGATTGTTGAGCAAGG+AGG - tig0023324:43674-43693 Msa1407750:intron 45.0%
GTTAATCCTGCATAGAGAGC+AGG + tig0023324:43704-43723 None:intergenic 45.0%
TCTAATCTGGATCATGGCTC+AGG + tig0023324:43824-43843 None:intergenic 45.0%
AATGTGTGATTCCCTTACCG+TGG - tig0023324:43944-43963 Msa1407750:intron 45.0%
ATTCCCTTACCGTGGTGAAT+TGG - tig0023324:43952-43971 Msa1407750:intron 45.0%
TTCCCTTACCGTGGTGAATT+GGG - tig0023324:43953-43972 Msa1407750:intron 45.0%
ATCCCAATTCACCACGGTAA+GGG + tig0023324:43958-43977 None:intergenic 45.0%
! TATATGTCGAGTTGTCGACC+AGG - tig0023324:44118-44137 Msa1407750:intron 45.0%
GAATCCCAAAGTCCATAGGA+TGG - tig0023324:44166-44185 Msa1407750:CDS 45.0%
CGACCATATAGGACAAGTAC+TGG - tig0023324:44238-44257 Msa1407750:intron 45.0%
GTCTCATTGGATTCTACTGC+TGG - tig0023324:44627-44646 Msa1407750:five_prime_UTR 45.0%
AGCAGTAGGAATCTAACCCT+TGG - tig0023324:44758-44777 Msa1407750:five_prime_UTR 45.0%
GTAGTATGCAGGATGTCCAA+GGG + tig0023324:44777-44796 None:intergenic 45.0%
AGTAGTATGCAGGATGTCCA+AGG + tig0023324:44778-44797 None:intergenic 45.0%
TGTGCTGTAAGGTCTGCTGT+TGG + tig0023324:41005-41024 None:intergenic 50.0%
CGCCTCTTCATTGTGCTGTA+AGG + tig0023324:41016-41035 None:intergenic 50.0%
ACAGCACAATGAAGAGGCGA+CGG - tig0023324:41017-41036 Msa1407750:three_prime_UTR 50.0%
TGTTGGAAGAGCCGTTGATC+TGG + tig0023324:41167-41186 None:intergenic 50.0%
GCATGAAGGAAAAGACCACC+TGG + tig0023324:41334-41353 None:intergenic 50.0%
GAAGGAGGTAGGAACAGTGT+TGG + tig0023324:41356-41375 None:intergenic 50.0%
CTGCAGGTAATGAAGGAGGT+AGG + tig0023324:41367-41386 None:intergenic 50.0%
GATGCTGCAGGTAATGAAGG+AGG + tig0023324:41371-41390 None:intergenic 50.0%
TGGGATGCTGCAGGTAATGA+AGG + tig0023324:41374-41393 None:intergenic 50.0%
TGAAGAAGATGGGATGCTGC+AGG + tig0023324:41383-41402 None:intergenic 50.0%
!! CTTAAGGTTCAAGGTGCTGC+TGG - tig0023324:41466-41485 Msa1407750:intron 50.0%
ATGACCAAACACCTCTGGTC+TGG - tig0023324:41651-41670 Msa1407750:three_prime_UTR 50.0%
AGAAGACCTTGAGACCATGG+CGG + tig0023324:41685-41704 None:intergenic 50.0%
AACCTTGCTCTGCTTGCCTA+TGG - tig0023324:42617-42636 Msa1407750:intron 50.0%
GACACTGTGAGTGCAAACTC+AGG - tig0023324:42639-42658 Msa1407750:intron 50.0%
ACGCACCTCCTACCATTTAG+TGG - tig0023324:43157-43176 Msa1407750:intron 50.0%
AGGACAGTAATGGTAGCACC+TGG - tig0023324:43275-43294 Msa1407750:CDS 50.0%
ACAGTAATGGTAGCACCTGG+CGG - tig0023324:43278-43297 Msa1407750:CDS 50.0%
!! GGTGCCATGCTGATAATGGA+GGG + tig0023324:43386-43405 None:intergenic 50.0%
!! AGGTGCCATGCTGATAATGG+AGG + tig0023324:43387-43406 None:intergenic 50.0%
! CCTTCAGGTGCTGTCTACTA+CGG - tig0023324:43404-43423 Msa1407750:intron 50.0%
AGCATATCTGCATTCACCCG+AGG - tig0023324:43448-43467 Msa1407750:CDS 50.0%
GCATATCTGCATTCACCCGA+GGG - tig0023324:43449-43468 Msa1407750:CDS 50.0%
GGGTCAAGAGCTGAATGCTT+TGG - tig0023324:43502-43521 Msa1407750:intron 50.0%
ATCTGGATCATGGCTCAGGT+AGG + tig0023324:43820-43839 None:intergenic 50.0%
GATCCCAATTCACCACGGTA+AGG + tig0023324:43959-43978 None:intergenic 50.0%
GCATGGATCCCAATTCACCA+CGG + tig0023324:43964-43983 None:intergenic 50.0%
TGCAAGAAAACAGAGGAGGG+AGG - tig0023324:44086-44105 Msa1407750:intron 50.0%
GCCCCAGTACTTGTCCTATA+TGG + tig0023324:44244-44263 None:intergenic 50.0%
TGAGTAATCGGCCACCTCTT+AGG + tig0023324:44570-44589 None:intergenic 50.0%
GCTGTAAGGTCTGCTGTTGG+TGG + tig0023324:41002-41021 None:intergenic 55.0%
AGAAGGACGAGCCGATCGAA+AGG + tig0023324:41125-41144 None:intergenic 55.0%
!! AAGGTTCAAGGTGCTGCTGG+TGG - tig0023324:41469-41488 Msa1407750:intron 55.0%
!! AGCACCAGACCAGAGGTGTT+TGG + tig0023324:41658-41677 None:intergenic 55.0%
! CTTTTGCCGCCATGGTCTCA+AGG - tig0023324:41676-41695 Msa1407750:three_prime_UTR 55.0%
TCTTCGGACGGCACCCTTTA+AGG - tig0023324:42479-42498 Msa1407750:CDS 55.0%
!! TTTGTTGCTGGTGCTGCTGC+AGG - tig0023324:42585-42604 Msa1407750:intron 55.0%
!! TTGTTGCTGGTGCTGCTGCA+GGG - tig0023324:42586-42605 Msa1407750:intron 55.0%
GTCCATAGGCAAGCAGAGCA+AGG + tig0023324:42622-42641 None:intergenic 55.0%
CCGTAGTAGACAGCACCTGA+AGG + tig0023324:43407-43426 None:intergenic 55.0%
GGTGTTCCTGCTCTCTATGC+AGG - tig0023324:43695-43714 Msa1407750:intron 55.0%
GTTGTCGACCAGGTGTGGTT+TGG - tig0023324:44128-44147 Msa1407750:CDS 55.0%
CTCCACTTGTGTCCACACCA+TGG - tig0023324:44543-44562 Msa1407750:exon 55.0%
GTCCATGGTGTGGACACAAG+TGG + tig0023324:44548-44567 None:intergenic 55.0%
GTCCACACCATGGACCTAAG+AGG - tig0023324:44553-44572 Msa1407750:five_prime_UTR 55.0%
CACCTCTTAGGTCCATGGTG+TGG + tig0023324:44558-44577 None:intergenic 55.0%
CACACCATGGACCTAAGAGG+TGG - tig0023324:44556-44575 Msa1407750:five_prime_UTR 55.0%
GCACAATGAAGAGGCGACGG+TGG - tig0023324:41020-41039 Msa1407750:three_prime_UTR 60.0%
!! CGGCAAAAGCACCAGACCAG+AGG + tig0023324:41665-41684 None:intergenic 60.0%
! GTCTGGTGCTTTTGCCGCCA+TGG - tig0023324:41668-41687 Msa1407750:three_prime_UTR 60.0%
!! TGTTGCTGGTGCTGCTGCAG+GGG - tig0023324:42587-42606 Msa1407750:intron 60.0%
AGCACCTGGCGGTGAAGCAT+TGG - tig0023324:43289-43308 Msa1407750:CDS 60.0%
! GCACCTGGCGGTGAAGCATT+GGG - tig0023324:43290-43309 Msa1407750:CDS 60.0%
! GTCGAGTTGTCGACCAGGTG+TGG - tig0023324:44123-44142 Msa1407750:intron 60.0%
!! TAGGACAAGTACTGGGGCCC+TGG - tig0023324:44246-44265 Msa1407750:CDS 60.0%
TCGGCCACCTCTTAGGTCCA+TGG + tig0023324:44563-44582 None:intergenic 60.0%
CCTCCCAATGCTTCACCGCC+AGG + tig0023324:43296-43315 None:intergenic 65.0%
!! CCTGGCGGTGAAGCATTGGG+AGG - tig0023324:43293-43312 Msa1407750:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0023324 gene 40994 44831 40994 ID=Msa1407750;Name=Msa1407750
tig0023324 mRNA 40994 44831 40994 ID=Msa1407750-mRNA-1;Parent=Msa1407750;Name=Msa1407750-mRNA-1;_AED=0.03;_eAED=0.03;_QI=283|0.8|0.66|1|0.2|0|6|1003|369
tig0023324 exon 40994 41474 40994 ID=Msa1407750-mRNA-1:exon:25278;Parent=Msa1407750-mRNA-1
tig0023324 exon 41558 42553 41558 ID=Msa1407750-mRNA-1:exon:25277;Parent=Msa1407750-mRNA-1
tig0023324 exon 43181 43383 43181 ID=Msa1407750-mRNA-1:exon:25276;Parent=Msa1407750-mRNA-1
tig0023324 exon 43435 43484 43435 ID=Msa1407750-mRNA-1:exon:25275;Parent=Msa1407750-mRNA-1
tig0023324 exon 44128 44207 44128 ID=Msa1407750-mRNA-1:exon:25274;Parent=Msa1407750-mRNA-1
tig0023324 exon 44246 44831 44246 ID=Msa1407750-mRNA-1:exon:25273;Parent=Msa1407750-mRNA-1
tig0023324 five_prime_UTR 44549 44831 44549 ID=Msa1407750-mRNA-1:five_prime_utr;Parent=Msa1407750-mRNA-1
tig0023324 CDS 44246 44548 44246 ID=Msa1407750-mRNA-1:cds;Parent=Msa1407750-mRNA-1
tig0023324 CDS 44128 44207 44128 ID=Msa1407750-mRNA-1:cds;Parent=Msa1407750-mRNA-1
tig0023324 CDS 43435 43484 43435 ID=Msa1407750-mRNA-1:cds;Parent=Msa1407750-mRNA-1
tig0023324 CDS 43181 43383 43181 ID=Msa1407750-mRNA-1:cds;Parent=Msa1407750-mRNA-1
tig0023324 CDS 42080 42553 42080 ID=Msa1407750-mRNA-1:cds;Parent=Msa1407750-mRNA-1
tig0023324 three_prime_UTR 41558 42079 41558 ID=Msa1407750-mRNA-1:three_prime_utr;Parent=Msa1407750-mRNA-1
tig0023324 three_prime_UTR 40994 41474 40994 ID=Msa1407750-mRNA-1:three_prime_utr;Parent=Msa1407750-mRNA-1
Gene Sequence

>Msa1407750

ATGTCCCTCTTGTCCAAACAAAACCAAGAACCCAACTCTGATTTGTTCTTCTTCCCAGGTGGTCTTTTCCTTCATGCCAACACTGTTCCTACCTCCTTCATTACCTGCAGCATCCCATCTTCTTCATCCAATTCTACCACTGTTTCCTGTTTTTCTTGGAATATCAGGAAAAGTGTCGGTTTTGAGATTCTTAAGGTTCAAGGTGCTGCTGGTGGTGTTTTCTCGTCCTTGAGTTTGTCTATAAACGGAAGAAGCGATGATGTAGATCATAGATACAAATCCAATCAAGTTTCAGAGCAGAAAAATATTCAGGTTCATGGATCGGGTGCTGTCAACATGACCAAACACCTCTGGTCTGGTGCTTTTGCCGCCATGGTCTCAAGGACTTTTGTTGCACCACTTGAGAGACTTAAGCTGGAATACATAGTTCGTGGTTTGAAAGGATTTTGGAAAGGAAATTTTGTGAATATTCTTCGGACGGCACCCTTTAAGGCTATAAATTTTTATGCCTATGATACATATAAAAATAAACTAGTCAGAATATCAGGGAATGAAGAATCCACAAATTTTGAGAGATTTGTTGCTGGTGCTGCTGCAGGGGTTACAGCAACCTTGCTCTGCTTGCCTATGGACACTATCAGGACAGTAATGGTAGCACCTGGCGGTGAAGCATTGGGAGGTGTAATCGGTGTCTTCCGCCATATGATCAAAACTGAGGGCTTCTTTTCTCTTTACAAAGGTTTAGTACCCTCCATTATCAGCATGGCACCTTCAGGTGCTGTCTACTACGGCGTTTATGATATTTTAAAGTCAGCATATCTGCATTCACCCGAGGGAATGAAAAGACTTCAATATATGAAAGAAGAGGGTCAAGAGCTGAATGCTTTGGAACAACTGGAATTGGGTACCATTAGAACTTTATTATATGGTGCTATTGCGGGTTGTTGTTCTGAAGCTGCTACATATCCCTTTGAAGTTGTAAGGAGACAGCTTCAATTGCAGGTTAGGGCAACAAGATTGAACGCGCTAGCGACTTGTGTCAAGATTGTTGAGCAAGGAGGTGTTCCTGCTCTCTATGCAGGATTAACTCCCAGCTTATTGCAGGTATAA
Protein sequence

>Msa1407750

MSLLSKQNQEPNSDLFFFPGGLFLHANTVPTSFITCSIPSSSSNSTTVSCFSWNIRKSVGFEILKVQGAAGGVFSSLSLSINGRSDDVDHRYKSNQVSEQKNIQVHGSGAVNMTKHLWSGAFAAMVSRTFVAPLERLKLEYIVRGLKGFWKGNFVNILRTAPFKAINFYAYDTYKNKLVRISGNEESTNFERFVAGAAAGVTATLLCLPMDTIRTVMVAPGGEALGGVIGVFRHMIKTEGFFSLYKGLVPSIISMAPSGAVYYGVYDILKSAYLHSPEGMKRLQYMKEEGQELNALEQLELGTIRTLLYGAIAGCCSEAATYPFEVVRRQLQLQVRATRLNALATCVKIVEQGGVPALYAGLTPSLLQV*