AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0571600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0571600 MtrunA17_Chr8g0386941 90.706 581 45 1 1 581 1 572 0.0 1033
Msa0571600 MtrunA17_Chr7g0247821 30.000 160 106 3 428 581 753 912 3.32e-13 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 92 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACTAACATGGACATTAAT+TGG 0.098262 4_1:+74293636 None:intergenic
ACAAGCTGTTGTAATGTTAT+TGG 0.222383 4_1:+74292727 None:intergenic
ACAACACATGCTTGGAAGTT+TGG 0.253601 4_1:+74292814 None:intergenic
AAGCACATCTGAACCAATTC+TGG 0.268014 4_1:+74295005 None:intergenic
TGTGGCATGGTTCTTAATAT+TGG 0.287551 4_1:-74295432 None:intergenic
CCATCAATAAGTTCGATAAA+TGG 0.291757 4_1:+74292977 None:intergenic
TGTTGTGCATTATTTGTATC+AGG 0.297508 4_1:+74293876 None:intergenic
TTTATCCAACTGCCTACTAT+TGG 0.307894 4_1:+74295270 None:intergenic
ACTATAACCTCTTCTTCTTC+TGG 0.312350 4_1:+74294265 None:intergenic
AGACCTTATAACATCCTCTT+TGG 0.324442 4_1:-74293079 Msa0571600:CDS
ATATCCTTTGACTTGTTCAT+TGG 0.329729 4_1:+74293054 None:intergenic
GAATGATCGGATAGCAGATT+TGG 0.343186 4_1:-74294222 Msa0571600:CDS
CCATTTATCGAACTTATTGA+TGG 0.346534 4_1:-74292977 Msa0571600:CDS
TTCCAAGTCCTGCGAACTGC+TGG 0.360062 4_1:+74293704 None:intergenic
TACTAACATGGACATTAATT+GGG 0.364399 4_1:+74293637 None:intergenic
TCACCAAATGTAAAATTTGA+AGG 0.376315 4_1:+74293813 None:intergenic
TAATCTTCGTGAGGCTCATT+CGG 0.396050 4_1:-74295308 Msa0571600:CDS
TCCCAGCAGTTCGCAGGACT+TGG 0.403469 4_1:-74293706 Msa0571600:CDS
AAATGTGAATGGCGAATGTT+TGG 0.414827 4_1:-74295337 Msa0571600:CDS
CATCTCTATGAAGTGCTTTG+AGG 0.416519 4_1:+74293475 None:intergenic
TTCGATAAATGGCTCTTCAT+AGG 0.422054 4_1:+74292988 None:intergenic
ATTACAACAGCTTGTTGAAA+GGG 0.423151 4_1:-74292719 Msa0571600:CDS
CATTCTATTTCTATCTTCCA+TGG 0.427164 4_1:+74292460 None:intergenic
TCAAAACTTACATCAACATC+AGG 0.435516 4_1:+74294961 None:intergenic
CAAGTGAAAGCAGAACAAAA+TGG 0.438289 4_1:-74294002 Msa0571600:CDS
GTATATCATCGAATGTTTCA+TGG 0.442276 4_1:-74292884 Msa0571600:CDS
TTTCACACCTTGGTCCTTCA+CGG 0.447121 4_1:+74293527 None:intergenic
TCCATTCACTTCATCAAAAG+TGG 0.447779 4_1:+74292945 None:intergenic
GATCCAAAGAGGATGTTATA+AGG 0.448481 4_1:+74293076 None:intergenic
CCTGAGACAAAATGACTTCA+TGG 0.448835 4_1:+74292485 None:intergenic
TTGCAACTCATTACTAAAAG+TGG 0.454464 4_1:+74293395 None:intergenic
TAAGACATGTCATGAGAGTA+AGG 0.458092 4_1:-74293776 Msa0571600:CDS
GGAAATGGGAAATCCAGAAT+TGG 0.460733 4_1:-74295018 Msa0571600:CDS
ATGTGTTGTGAGGAAAGTTT+TGG 0.462231 4_1:-74292800 Msa0571600:CDS
TAGAACATTAATCAATAACT+TGG 0.472348 4_1:-74292197 Msa0571600:three_prime_UTR
CTTTCCTCACAACACATGCT+TGG 0.474299 4_1:+74292806 None:intergenic
CTCAAAGCACTTCATAGAGA+TGG 0.475720 4_1:-74293474 Msa0571600:CDS
TATCTTTCGATTGGAGAAAG+AGG 0.494534 4_1:+74292284 None:intergenic
TCAAAGCACTTCATAGAGAT+GGG 0.495966 4_1:-74293473 Msa0571600:CDS
TGGGTAGTTGCTCATGGTGG+TGG 0.508287 4_1:-74292519 Msa0571600:three_prime_UTR
GCAGTGCGGGACAACTGATT+CGG 0.510006 4_1:-74293742 Msa0571600:CDS
AAAATTTCCCAGCAGTTCGC+AGG 0.510618 4_1:-74293712 Msa0571600:CDS
CATTACAACAGCTTGTTGAA+AGG 0.512663 4_1:-74292720 Msa0571600:CDS
CACTTTGCTGACACTAGCTC+TGG 0.514081 4_1:-74293855 Msa0571600:CDS
ATAATGTGCATGCAAATTGT+CGG 0.521272 4_1:+74293329 None:intergenic
TGTTGTAATGTTATTGGTGA+TGG 0.522358 4_1:+74292733 None:intergenic
CAATTTCTTCTGCATCAACA+TGG 0.524519 4_1:+74292665 None:intergenic
ACTTGGCCAGACGTGAATCA+TGG 0.524559 4_1:-74292696 Msa0571600:CDS
AGTCATCCATGATTCACGTC+TGG 0.529300 4_1:+74292690 None:intergenic
GGAGAAAAGAACAGCAGTGC+GGG 0.535867 4_1:-74293755 Msa0571600:CDS
GGTTTCCAATAGTAGGCAGT+TGG 0.538036 4_1:-74295275 Msa0571600:CDS
TCCAAGTCCTGCGAACTGCT+GGG 0.538815 4_1:+74293705 None:intergenic
ATCATAAATTTGATCGCCGT+GGG 0.540757 4_1:-74292538 Msa0571600:three_prime_UTR
ATGTTATAAGGTCTTCAAGA+TGG 0.541014 4_1:+74293088 None:intergenic
GGCTCATTCGGATGGAAGAT+CGG 0.542039 4_1:-74295296 Msa0571600:CDS
CTTGTTCATTGGATCCAAAG+AGG 0.545524 4_1:+74293065 None:intergenic
CTTCGTGAGGCTCATTCGGA+TGG 0.545723 4_1:-74295304 Msa0571600:CDS
GAGATAAAACTTTCACACCT+TGG 0.547276 4_1:+74293517 None:intergenic
GGACAGAAGATCTCCTCCTC+TGG 0.548594 4_1:-74295368 Msa0571600:CDS
GAAGATCGGTTTCCAATAGT+AGG 0.549773 4_1:-74295282 Msa0571600:CDS
ATTATTGAGAGTGATGCATA+TGG 0.552597 4_1:-74292312 Msa0571600:three_prime_UTR
AGTTCGCAGGACTTGGAAGA+TGG 0.556294 4_1:-74293699 Msa0571600:CDS
GCAAGATCCTAACTCTCTGT+TGG 0.561889 4_1:-74294054 Msa0571600:CDS
ATCTTTCGATTGGAGAAAGA+GGG 0.568464 4_1:+74292285 None:intergenic
AATCATAAATTTGATCGCCG+TGG 0.569644 4_1:-74292539 Msa0571600:three_prime_UTR
AATGTTATTGGTGATGGATG+AGG 0.570884 4_1:+74292739 None:intergenic
GTATGCTCCAGAAGAAGAAG+AGG 0.572437 4_1:-74294272 Msa0571600:CDS
TCGCCGTGGGTAGTTGCTCA+TGG 0.576814 4_1:-74292525 Msa0571600:three_prime_UTR
TGGAATCTTTAATCTTCGTG+AGG 0.576871 4_1:-74295317 Msa0571600:CDS
ACAGCTTGTTGAAAGGGACT+TGG 0.590760 4_1:-74292713 Msa0571600:CDS
GCAAAATAAATTGACCGTGA+AGG 0.591471 4_1:-74293541 Msa0571600:CDS
GGATCCAATGAACAAGTCAA+AGG 0.593979 4_1:-74293058 Msa0571600:CDS
GTGTACGTGTGGAGAATGAT+CGG 0.594868 4_1:-74294235 Msa0571600:CDS
GATGATATACTCATGCAAGA+TGG 0.600077 4_1:+74292897 None:intergenic
ATGTTATTGGTGATGGATGA+GGG 0.600743 4_1:+74292740 None:intergenic
AGAAGAAATTGCAATGGAGG+TGG 0.605756 4_1:-74292653 Msa0571600:CDS
ACTAACATGGACATTAATTG+GGG 0.615194 4_1:+74293638 None:intergenic
TCATGGTGGTGGAGACCGCA+TGG 0.624096 4_1:-74292508 Msa0571600:three_prime_UTR
CCACCATGAGCAACTACCCA+CGG 0.624645 4_1:+74292522 None:intergenic
ACTTCCAAGCATGTGTTGTG+AGG 0.626643 4_1:-74292810 Msa0571600:CDS
CAAAACTTACATCAACATCA+GGG 0.632189 4_1:+74294962 None:intergenic
AAATTGACCGTGAAGGACCA+AGG 0.639782 4_1:-74293534 Msa0571600:CDS
TAGTTGTAAGAGTGTACGTG+TGG 0.641450 4_1:-74294246 Msa0571600:CDS
TGCAGAAGAAATTGCAATGG+AGG 0.645966 4_1:-74292656 Msa0571600:CDS
GCACATTATGATATTCCTAG+AGG 0.662018 4_1:-74293315 Msa0571600:intron
CAAAGCACTTCATAGAGATG+GGG 0.670872 4_1:-74293472 Msa0571600:CDS
TGATGCAGAAGAAATTGCAA+TGG 0.674423 4_1:-74292659 Msa0571600:CDS
CCGTGGGTAGTTGCTCATGG+TGG 0.698035 4_1:-74292522 Msa0571600:three_prime_UTR
AGGAGAAAAGAACAGCAGTG+CGG 0.716182 4_1:-74293756 Msa0571600:CDS
AAAATGACTTCATGGCCATG+CGG 0.723331 4_1:+74292493 None:intergenic
CAGTTGGATAAACACACCAG+TGG 0.724616 4_1:-74295259 Msa0571600:intron
ATTGTTTCTATGTGACTGTG+AGG 0.735063 4_1:+74292396 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGAAGAAAACTATTTTTT+AGG + chr4_1:74292199-74292218 None:intergenic 15.0%
!! TATTATACTCATCTTAAATT+TGG - chr4_1:74292524-74292543 Msa0571600:three_prime_UTR 15.0%
!! TGTTAATAAATATCTAGAAA+GGG - chr4_1:74292874-74292893 Msa0571600:CDS 15.0%
!! TATTATTGTATTATGGTTAA+TGG + chr4_1:74292979-74292998 None:intergenic 15.0%
!!! AAAATAGTTTTCTTCAATCA+TGG - chr4_1:74292201-74292220 Msa0571600:three_prime_UTR 20.0%
!!! TTCTTCTTTTTTAGCTTAAA+AGG - chr4_1:74292360-74292379 Msa0571600:three_prime_UTR 20.0%
!!! TGAATCAAATTTTGATTTTC+TGG - chr4_1:74292491-74292510 Msa0571600:three_prime_UTR 20.0%
!!! GAATCAAATTTTGATTTTCT+GGG - chr4_1:74292492-74292511 Msa0571600:three_prime_UTR 20.0%
!! CTGTTAATAAATATCTAGAA+AGG - chr4_1:74292873-74292892 Msa0571600:CDS 20.0%
!! GTTAATAAATATCTAGAAAG+GGG - chr4_1:74292875-74292894 Msa0571600:CDS 20.0%
!! AAGGCAATATTATTGTATTA+TGG + chr4_1:74292986-74293005 None:intergenic 20.0%
!!! ATAGCAAAGGTTTAAAATAT+CGG + chr4_1:74293143-74293162 None:intergenic 20.0%
!!! TTTAAAATGTCATGTATAGT+TGG - chr4_1:74293221-74293240 Msa0571600:intron 20.0%
!! TACTAAATCATTCATGTAAT+GGG + chr4_1:74293266-74293285 None:intergenic 20.0%
!! TGGTAAAAATGTAAAAAAGT+TGG - chr4_1:74293911-74293930 Msa0571600:CDS 20.0%
!! AAAAAACTAGTTATATAACG+AGG + chr4_1:74294305-74294324 None:intergenic 20.0%
!! TATTGATCAATTCTTTAGTA+TGG - chr4_1:74294379-74294398 Msa0571600:intron 20.0%
!!! TTCTAAAAATATCTTTCGAT+TGG + chr4_1:74295318-74295337 None:intergenic 20.0%
!! TAGAACATTAATCAATAACT+TGG - chr4_1:74295393-74295412 Msa0571600:five_prime_UTR 20.0%
! CAAATGATATTTATGGTTAC+TGG + chr4_1:74292457-74292476 None:intergenic 25.0%
! ACAGCTTCAAATGATATTTA+TGG + chr4_1:74292464-74292483 None:intergenic 25.0%
! ACTAAATCATTCATGTAATG+GGG + chr4_1:74293265-74293284 None:intergenic 25.0%
! CTACTAAATCATTCATGTAA+TGG + chr4_1:74293267-74293286 None:intergenic 25.0%
! ATTTAGTAGCATAACATTGA+TGG - chr4_1:74293278-74293297 Msa0571600:intron 25.0%
!!! TTTTTCAACAAAAACTCTCA+AGG - chr4_1:74293426-74293445 Msa0571600:CDS 25.0%
!!! CGAAACTACTATTTTTTTAG+AGG + chr4_1:74293691-74293710 None:intergenic 25.0%
!! AAGCCTTCAAATTTTACATT+TGG - chr4_1:74293774-74293793 Msa0571600:CDS 25.0%
! TCACCAAATGTAAAATTTGA+AGG + chr4_1:74293780-74293799 None:intergenic 25.0%
! TACTAACATGGACATTAATT+GGG + chr4_1:74293956-74293975 None:intergenic 25.0%
!!! AACTTTTTTCACTACTAACA+TGG + chr4_1:74293968-74293987 None:intergenic 25.0%
!!! TTTGAAGTTTTTCAAGTTGA+AGG - chr4_1:74293986-74294005 Msa0571600:CDS 25.0%
! AAAAATCAAAGATTTGCATG+TGG - chr4_1:74294172-74294191 Msa0571600:CDS 25.0%
! CAATACATAGATGATAAATG+AGG + chr4_1:74294364-74294383 None:intergenic 25.0%
! GGATTTCTTGATGAAAAATA+GGG + chr4_1:74294758-74294777 None:intergenic 25.0%
! TGGATTTCTTGATGAAAAAT+AGG + chr4_1:74294759-74294778 None:intergenic 25.0%
TGGTTAACCAAAAATGTGAA+TGG - chr4_1:74292242-74292261 Msa0571600:three_prime_UTR 30.0%
! ACTCATCTTAAATTTGGAAC+TGG - chr4_1:74292530-74292549 Msa0571600:three_prime_UTR 30.0%
CAAAACTTACATCAACATCA+GGG + chr4_1:74292631-74292650 None:intergenic 30.0%
TCAAAACTTACATCAACATC+AGG + chr4_1:74292632-74292651 None:intergenic 30.0%
TTTGTAGCAGCTATTGTAAA+AGG - chr4_1:74292678-74292697 Msa0571600:CDS 30.0%
!!! TTAAACCCTTTTTAAAGCCT+CGG - chr4_1:74292752-74292771 Msa0571600:CDS 30.0%
ACTACTTCAGTAACTTAGAA+AGG + chr4_1:74292931-74292950 None:intergenic 30.0%
AATAATATTGCCTTGCTTTG+AGG - chr4_1:74292992-74293011 Msa0571600:CDS 30.0%
!! ATGAAACTAGTTGATAGCAA+AGG + chr4_1:74293156-74293175 None:intergenic 30.0%
! ACATAAACTAGAAGTATGTG+AGG - chr4_1:74293192-74293211 Msa0571600:intron 30.0%
! TTGTCAGCCTAATCAATTTT+TGG - chr4_1:74293464-74293483 Msa0571600:CDS 30.0%
ACAGCATCCAAAAATTGATT+AGG + chr4_1:74293474-74293493 None:intergenic 30.0%
!! ACAGGTTTTATATTCGAACA+AGG - chr4_1:74293494-74293513 Msa0571600:CDS 30.0%
!!! AAAACAGTAGTAGTTTTGAC+AGG - chr4_1:74293634-74293653 Msa0571600:CDS 30.0%
TGTTGTGCATTATTTGTATC+AGG + chr4_1:74293717-74293736 None:intergenic 30.0%
ACTAACATGGACATTAATTG+GGG + chr4_1:74293955-74293974 None:intergenic 30.0%
CTACTAACATGGACATTAAT+TGG + chr4_1:74293957-74293976 None:intergenic 30.0%
!!! GTTTTTCAAGTTGAAGGATT+CGG - chr4_1:74293992-74294011 Msa0571600:CDS 30.0%
TTGCAACTCATTACTAAAAG+TGG + chr4_1:74294198-74294217 None:intergenic 30.0%
! AGTAATGAGTTGCAAGTTTT+TGG - chr4_1:74294203-74294222 Msa0571600:CDS 30.0%
ATAATGTGCATGCAAATTGT+CGG + chr4_1:74294264-74294283 None:intergenic 30.0%
ATTCTTTAGTATGGTCTGAA+AGG - chr4_1:74294388-74294407 Msa0571600:intron 30.0%
ATGTTATAAGGTCTTCAAGA+TGG + chr4_1:74294505-74294524 None:intergenic 30.0%
!! ATATCCTTTGACTTGTTCAT+TGG + chr4_1:74294539-74294558 None:intergenic 30.0%
CCATCAATAAGTTCGATAAA+TGG + chr4_1:74294616-74294635 None:intergenic 30.0%
CCATTTATCGAACTTATTGA+TGG - chr4_1:74294613-74294632 Msa0571600:intron 30.0%
GTATATCATCGAATGTTTCA+TGG - chr4_1:74294706-74294725 Msa0571600:intron 30.0%
TGTTGTAATGTTATTGGTGA+TGG + chr4_1:74294860-74294879 None:intergenic 30.0%
ACAAGCTGTTGTAATGTTAT+TGG + chr4_1:74294866-74294885 None:intergenic 30.0%
!! ATTACAACAGCTTGTTGAAA+GGG - chr4_1:74294871-74294890 Msa0571600:intron 30.0%
!!! GAAAAATGTTTTGGAAACAC+TGG - chr4_1:74294961-74294980 Msa0571600:intron 30.0%
!! CATTCTATTTCTATCTTCCA+TGG + chr4_1:74295133-74295152 None:intergenic 30.0%
ATTATTGAGAGTGATGCATA+TGG - chr4_1:74295278-74295297 Msa0571600:CDS 30.0%
!!! TCACATTTTTGGTTAACCAG+AGG + chr4_1:74292241-74292260 None:intergenic 35.0%
! ACATTCGCCATTCACATTTT+TGG + chr4_1:74292252-74292271 None:intergenic 35.0%
AAATGTGAATGGCGAATGTT+TGG - chr4_1:74292253-74292272 Msa0571600:three_prime_UTR 35.0%
TGGAATCTTTAATCTTCGTG+AGG - chr4_1:74292273-74292292 Msa0571600:three_prime_UTR 35.0%
TTTATCCAACTGCCTACTAT+TGG + chr4_1:74292323-74292342 None:intergenic 35.0%
AAAAGAAGAACTCTTACCAC+TGG + chr4_1:74292350-74292369 None:intergenic 35.0%
TAGACTTTCAGTGCATACAA+TGG + chr4_1:74292425-74292444 None:intergenic 35.0%
TCATACATGCTTGTGATTTC+AGG + chr4_1:74292729-74292748 None:intergenic 35.0%
ACTATAACCTCTTCTTCTTC+TGG + chr4_1:74293328-74293347 None:intergenic 35.0%
AAAAACTCTCAAGGCAAAGA+TGG - chr4_1:74293435-74293454 Msa0571600:CDS 35.0%
!! TCAATTTTTGGATGCTGTAC+AGG - chr4_1:74293476-74293495 Msa0571600:CDS 35.0%
! TGTTTTACCAACAGAGAGTT+AGG + chr4_1:74293546-74293565 None:intergenic 35.0%
CAAGTGAAAGCAGAACAAAA+TGG - chr4_1:74293588-74293607 Msa0571600:CDS 35.0%
TAAGACATGTCATGAGAGTA+AGG - chr4_1:74293814-74293833 Msa0571600:CDS 35.0%
GCAAAATAAATTGACCGTGA+AGG - chr4_1:74294049-74294068 Msa0571600:CDS 35.0%
GAGATAAAACTTTCACACCT+TGG + chr4_1:74294076-74294095 None:intergenic 35.0%
ATCTCATAAAAAGCAGCAGA+TGG - chr4_1:74294091-74294110 Msa0571600:CDS 35.0%
! TCAAAGCACTTCATAGAGAT+GGG - chr4_1:74294117-74294136 Msa0571600:CDS 35.0%
GCACATTATGATATTCCTAG+AGG - chr4_1:74294275-74294294 Msa0571600:CDS 35.0%
! GTTTTCTTGCAGAAACAGAT+GGG - chr4_1:74294441-74294460 Msa0571600:intron 35.0%
AGACCTTATAACATCCTCTT+TGG - chr4_1:74294511-74294530 Msa0571600:intron 35.0%
GATCCAAAGAGGATGTTATA+AGG + chr4_1:74294517-74294536 None:intergenic 35.0%
TTCGATAAATGGCTCTTCAT+AGG + chr4_1:74294605-74294624 None:intergenic 35.0%
!! TCCACTTTTGATGAAGTGAA+TGG - chr4_1:74294644-74294663 Msa0571600:intron 35.0%
TCCATTCACTTCATCAAAAG+TGG + chr4_1:74294648-74294667 None:intergenic 35.0%
AGATGGTACTGTCAAAAAGT+TGG + chr4_1:74294679-74294698 None:intergenic 35.0%
GATGATATACTCATGCAAGA+TGG + chr4_1:74294696-74294715 None:intergenic 35.0%
AGGGTGAAAAATCACTATTC+TGG + chr4_1:74294739-74294758 None:intergenic 35.0%
! ATGTGTTGTGAGGAAAGTTT+TGG - chr4_1:74294790-74294809 Msa0571600:intron 35.0%
!! ATGTTATTGGTGATGGATGA+GGG + chr4_1:74294853-74294872 None:intergenic 35.0%
!! AATGTTATTGGTGATGGATG+AGG + chr4_1:74294854-74294873 None:intergenic 35.0%
!! CATTACAACAGCTTGTTGAA+AGG - chr4_1:74294870-74294889 Msa0571600:intron 35.0%
CAATTTCTTCTGCATCAACA+TGG + chr4_1:74294928-74294947 None:intergenic 35.0%
TGATGCAGAAGAAATTGCAA+TGG - chr4_1:74294931-74294950 Msa0571600:intron 35.0%
!!! TGTTTTGGAAACACTGGTTT+TGG - chr4_1:74294967-74294986 Msa0571600:intron 35.0%
AATCATAAATTTGATCGCCG+TGG - chr4_1:74295051-74295070 Msa0571600:intron 35.0%
ATCATAAATTTGATCGCCGT+GGG - chr4_1:74295052-74295071 Msa0571600:intron 35.0%
!! ATTGTTTCTATGTGACTGTG+AGG + chr4_1:74295197-74295216 None:intergenic 35.0%
ATCTTTCGATTGGAGAAAGA+GGG + chr4_1:74295308-74295327 None:intergenic 35.0%
TATCTTTCGATTGGAGAAAG+AGG + chr4_1:74295309-74295328 None:intergenic 35.0%
!!! CATTTTTGGTTAACCAGAGG+AGG + chr4_1:74292238-74292257 None:intergenic 40.0%
TAATCTTCGTGAGGCTCATT+CGG - chr4_1:74292282-74292301 Msa0571600:three_prime_UTR 40.0%
! GAAGATCGGTTTCCAATAGT+AGG - chr4_1:74292308-74292327 Msa0571600:three_prime_UTR 40.0%
! TTTTTCCAAGCTGCAGGAAA+TGG - chr4_1:74292557-74292576 Msa0571600:three_prime_UTR 40.0%
! TTTTCCAAGCTGCAGGAAAT+GGG - chr4_1:74292558-74292577 Msa0571600:three_prime_UTR 40.0%
GGAAATGGGAAATCCAGAAT+TGG - chr4_1:74292572-74292591 Msa0571600:three_prime_UTR 40.0%
AAGCACATCTGAACCAATTC+TGG + chr4_1:74292588-74292607 None:intergenic 40.0%
!! CTAGTCCGAGGCTTTAAAAA+GGG + chr4_1:74292760-74292779 None:intergenic 40.0%
!! ACTAGTCCGAGGCTTTAAAA+AGG + chr4_1:74292761-74292780 None:intergenic 40.0%
!!! TTTTTAAAGCCTCGGACTAG+TGG - chr4_1:74292760-74292779 Msa0571600:CDS 40.0%
AGAAAGGGGAAAGACTTTGA+AGG - chr4_1:74292889-74292908 Msa0571600:CDS 40.0%
ATCACTAGATCCTCAAAGCA+AGG + chr4_1:74293005-74293024 None:intergenic 40.0%
! CTTTGAGGATCTAGTGATCA+AGG - chr4_1:74293007-74293026 Msa0571600:CDS 40.0%
ACATAAAACTCGACATGACG+TGG + chr4_1:74293052-74293071 None:intergenic 40.0%
! TCATTCATGTAATGGGGTGA+GGG + chr4_1:74293259-74293278 None:intergenic 40.0%
! ATCATTCATGTAATGGGGTG+AGG + chr4_1:74293260-74293279 None:intergenic 40.0%
!!! GATGGAAGAATTTTGACTGC+AGG - chr4_1:74293296-74293315 Msa0571600:intron 40.0%
! TAGTTGTAAGAGTGTACGTG+TGG - chr4_1:74293344-74293363 Msa0571600:CDS 40.0%
GAATGATCGGATAGCAGATT+TGG - chr4_1:74293368-74293387 Msa0571600:CDS 40.0%
! GAACTGCTGGGAAATTTTCT+TGG + chr4_1:74293876-74293895 None:intergenic 40.0%
! CATCTCTATGAAGTGCTTTG+AGG + chr4_1:74294118-74294137 None:intergenic 40.0%
! CTCAAAGCACTTCATAGAGA+TGG - chr4_1:74294116-74294135 Msa0571600:CDS 40.0%
! CAAAGCACTTCATAGAGATG+GGG - chr4_1:74294118-74294137 Msa0571600:CDS 40.0%
ATATAACGAGGCATACCTCT+AGG + chr4_1:74294293-74294312 None:intergenic 40.0%
! CGTTTTCTTGCAGAAACAGA+TGG - chr4_1:74294440-74294459 Msa0571600:intron 40.0%
CTTGTTCATTGGATCCAAAG+AGG + chr4_1:74294528-74294547 None:intergenic 40.0%
GGATCCAATGAACAAGTCAA+AGG - chr4_1:74294532-74294551 Msa0571600:intron 40.0%
ACAACACATGCTTGGAAGTT+TGG + chr4_1:74294779-74294798 None:intergenic 40.0%
TGCAGAAGAAATTGCAATGG+AGG - chr4_1:74294934-74294953 Msa0571600:intron 40.0%
AGAAGAAATTGCAATGGAGG+TGG - chr4_1:74294937-74294956 Msa0571600:intron 40.0%
! GGAGGTGGAGAAAAATGTTT+TGG - chr4_1:74294952-74294971 Msa0571600:intron 40.0%
AAAATGACTTCATGGCCATG+CGG + chr4_1:74295100-74295119 None:intergenic 40.0%
CCTGAGACAAAATGACTTCA+TGG + chr4_1:74295108-74295127 None:intergenic 40.0%
!! CCATGAAGTCATTTTGTCTC+AGG - chr4_1:74295105-74295124 Msa0571600:intron 40.0%
! TCATTTTGTCTCAGGTTCCA+TGG - chr4_1:74295113-74295132 Msa0571600:intron 40.0%
GGTTTCCAATAGTAGGCAGT+TGG - chr4_1:74292315-74292334 Msa0571600:three_prime_UTR 45.0%
CAGTTGGATAAACACACCAG+TGG - chr4_1:74292331-74292350 Msa0571600:three_prime_UTR 45.0%
!!! GGTGATTTTTTCCAAGCTGC+AGG - chr4_1:74292551-74292570 Msa0571600:three_prime_UTR 45.0%
ATTTCCCATTTCCTGCAGCT+TGG + chr4_1:74292565-74292584 None:intergenic 45.0%
TTACCAAAACCACTAGTCCG+AGG + chr4_1:74292772-74292791 None:intergenic 45.0%
! CATTCATGTAATGGGGTGAG+GGG + chr4_1:74293258-74293277 None:intergenic 45.0%
GTATGCTCCAGAAGAAGAAG+AGG - chr4_1:74293318-74293337 Msa0571600:CDS 45.0%
GTGTACGTGTGGAGAATGAT+CGG - chr4_1:74293355-74293374 Msa0571600:CDS 45.0%
GCAAGATCCTAACTCTCTGT+TGG - chr4_1:74293536-74293555 Msa0571600:CDS 45.0%
AGGAGAAAAGAACAGCAGTG+CGG - chr4_1:74293834-74293853 Msa0571600:CDS 45.0%
AAAATTTCCCAGCAGTTCGC+AGG - chr4_1:74293878-74293897 Msa0571600:CDS 45.0%
AAATTGACCGTGAAGGACCA+AGG - chr4_1:74294056-74294075 Msa0571600:CDS 45.0%
TTTCACACCTTGGTCCTTCA+CGG + chr4_1:74294066-74294085 None:intergenic 45.0%
ACTTCCAAGCATGTGTTGTG+AGG - chr4_1:74294780-74294799 Msa0571600:intron 45.0%
CTTTCCTCACAACACATGCT+TGG + chr4_1:74294787-74294806 None:intergenic 45.0%
!! ACAGCTTGTTGAAAGGGACT+TGG - chr4_1:74294877-74294896 Msa0571600:intron 45.0%
AGTCATCCATGATTCACGTC+TGG + chr4_1:74294903-74294922 None:intergenic 45.0%
GGCTCATTCGGATGGAAGAT+CGG - chr4_1:74292294-74292313 Msa0571600:three_prime_UTR 50.0%
!!! AAGCCTCGGACTAGTGGTTT+TGG - chr4_1:74292766-74292785 Msa0571600:CDS 50.0%
GTGCTCAAATGAGCTCGATG+CGG + chr4_1:74293106-74293125 None:intergenic 50.0%
CACTTTGCTGACACTAGCTC+TGG - chr4_1:74293735-74293754 Msa0571600:CDS 50.0%
GGAGAAAAGAACAGCAGTGC+GGG - chr4_1:74293835-74293854 Msa0571600:CDS 50.0%
AGTTCGCAGGACTTGGAAGA+TGG - chr4_1:74293891-74293910 Msa0571600:CDS 50.0%
ACTTGGCCAGACGTGAATCA+TGG - chr4_1:74294894-74294913 Msa0571600:intron 50.0%
GGACAGAAGATCTCCTCCTC+TGG - chr4_1:74292222-74292241 Msa0571600:three_prime_UTR 55.0%
CTTCGTGAGGCTCATTCGGA+TGG - chr4_1:74292286-74292305 Msa0571600:three_prime_UTR 55.0%
GTGATCAAGGTCTGCGACCA+CGG - chr4_1:74293020-74293039 Msa0571600:CDS 55.0%
ACATGACGTGGACCAAACCG+TGG + chr4_1:74293040-74293059 None:intergenic 55.0%
CCTCAATCGTGGTCGTGTTG+CGG + chr4_1:74293083-74293102 None:intergenic 55.0%
GCAGTGCGGGACAACTGATT+CGG - chr4_1:74293848-74293867 Msa0571600:CDS 55.0%
TCCAAGTCCTGCGAACTGCT+GGG + chr4_1:74293888-74293907 None:intergenic 55.0%
TTCCAAGTCCTGCGAACTGC+TGG + chr4_1:74293889-74293908 None:intergenic 55.0%
CCACCATGAGCAACTACCCA+CGG + chr4_1:74295071-74295090 None:intergenic 55.0%
TGGGTAGTTGCTCATGGTGG+TGG - chr4_1:74295071-74295090 Msa0571600:intron 55.0%
CAAGGTCTGCGACCACGGTT+TGG - chr4_1:74293025-74293044 Msa0571600:CDS 60.0%
CCGCAACACGACCACGATTG+AGG - chr4_1:74293080-74293099 Msa0571600:CDS 60.0%
TCCCAGCAGTTCGCAGGACT+TGG - chr4_1:74293884-74293903 Msa0571600:CDS 60.0%
TCGCCGTGGGTAGTTGCTCA+TGG - chr4_1:74295065-74295084 Msa0571600:intron 60.0%
CCGTGGGTAGTTGCTCATGG+TGG - chr4_1:74295068-74295087 Msa0571600:intron 60.0%
!! TCATGGTGGTGGAGACCGCA+TGG - chr4_1:74295082-74295101 Msa0571600:intron 60.0%
GCTCGATGCGGCCTCAATCG+TGG + chr4_1:74293094-74293113 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 74292172 74295440 74292172 ID=Msa0571600;Name=Msa0571600
chr4_1 mRNA 74292172 74295440 74292172 ID=Msa0571600-mRNA-1;Parent=Msa0571600;Name=Msa0571600-mRNA-1;_AED=0.03;_eAED=0.03;_QI=48|1|1|1|1|1|4|422|581
chr4_1 exon 74292172 74293159 74292172 ID=Msa0571600-mRNA-1:exon:25629;Parent=Msa0571600-mRNA-1
chr4_1 exon 74293316 74294294 74293316 ID=Msa0571600-mRNA-1:exon:25628;Parent=Msa0571600-mRNA-1
chr4_1 exon 74294972 74295039 74294972 ID=Msa0571600-mRNA-1:exon:25627;Parent=Msa0571600-mRNA-1
chr4_1 exon 74295260 74295440 74295260 ID=Msa0571600-mRNA-1:exon:25626;Parent=Msa0571600-mRNA-1
chr4_1 five_prime_UTR 74295393 74295440 74295393 ID=Msa0571600-mRNA-1:five_prime_utr;Parent=Msa0571600-mRNA-1
chr4_1 CDS 74295260 74295392 74295260 ID=Msa0571600-mRNA-1:cds;Parent=Msa0571600-mRNA-1
chr4_1 CDS 74294972 74295039 74294972 ID=Msa0571600-mRNA-1:cds;Parent=Msa0571600-mRNA-1
chr4_1 CDS 74293316 74294294 74293316 ID=Msa0571600-mRNA-1:cds;Parent=Msa0571600-mRNA-1
chr4_1 CDS 74292594 74293159 74292594 ID=Msa0571600-mRNA-1:cds;Parent=Msa0571600-mRNA-1
chr4_1 three_prime_UTR 74292172 74292593 74292172 ID=Msa0571600-mRNA-1:three_prime_utr;Parent=Msa0571600-mRNA-1
Gene Sequence

>Msa0571600

ATGGACAGAAGATCTCCTCCTCTGGTTAACCAAAAATGTGAATGGCGAATGTTTGGAATCTTTAATCTTCGTGAGGCTCATTCGGATGGAAGATCGGTTTCCAATAGTAGGCAGTTGGATAAACACACCAGTGGAAATGGGAAATCCAGAATTGGTTCAGATGTGCTTAGCACAGTTGATGAAAAGTACCCTGATGTTGATGTATGCTCCAGAAGAAGAAGAGGTTATAGTTGTAAGAGTGTACGTGTGGAGAATGATCGGATAGCAGATTTGGAAAATGAAGTAACAAAAATGATTGTTAATCAGAGATTTTTCAACAAAAACTCTCAAGGCAAAGATGGTGCAGATTGTCAGCCTAATCAATTTTTGGATGCTGTACAGGTTTTATATTCGAACAAGGAACTTTTCACAAAACTTCTGCAAGATCCTAACTCTCTGTTGGTAAAACAGATTCATGACATACAGAAAACTCAAGTGAAAGCAGAACAAAATGGAGTAATAAGATTGAATAAAGCTCAAAACAGTAGTAGTTTTGACAGGTTTAATTCGAGTTCAAATTGTGAGTCTCAGTCCTCTAAAAAAATAGTAGTTTCGAAGCCTGATACAAATAATGCACAACACTTTGCTGACACTAGCTCTGGCAATGCTCAAAATATTAAGCCTTCAAATTTTACATTTGGTGAAATAAAGAGAAAGTTAAGACATGTCATGAGAGTAAGGAGAAAAGAACAGCAGTGCGGGACAACTGATTCGGCGCCAAGAAAATTTCCCAGCAGTTCGCAGGACTTGGAAGATGGTAAAAATGTAAAAAAGTTGGACACTTCTGAAAGAAATTCCCCAATTAATGTCCATGTTAGTAGTGAAAAAAGTTTGAAGTTTTTCAAGTTGAAGGATTCGGAGTCAACTATGAGACAAGAAGAAGTTGCTTCTGAGCAAAATAAATTGACCGTGAAGGACCAAGGTGTGAAAGTTTTATCTCATAAAAAGCAGCAGATGGTCCTCAAAGCACTTCATAGAGATGGGGAAAGTTGCTCTTATAGTAGTTCTTCAGCTCAAAAAATCAAAGATTTGCATGTGGCCACTTTTAGTAATGAGTTGCAAGTTTTTGGTGCTGCAGATATTAGTGTCAATAAGAGTCTTTCTGCCGACAATTTGCATGCACATTATGATATTCCTAGAGAAACAGATGGGTCACTAGTACAAGTTGATTATGATAGATTTGAAGAGAACCATCTTGAAGACCTTATAACATCCTCTTTGGATCCAATGAACAAGTCAAAGGATATCATAACTGAAGTTCTGCAGACTTTCAGCTTGAAATGCGATGAGCCTATGAAGAGCCATTTATCGAACTTATTGATGGATTCTTCATCCACTTTTGATGAAGTGAATGGATTGACTGACCAACTTTTTGACAGTACCATCTTGCATGAGTATATCATCGAATGTTTCATGGAGTTATACCAGAATAGTGATTTTTCACCCTATTTTTCATCAAGAAATCCAAACTTCCAAGCATGTGTTGTGAGGAAAGTTTTGGTTAGACAGATTAATGAACTTGTTAAACTTCACTTTTTCCCTCATCCATCACCAATAACATTACAACAGCTTGTTGAAAGGGACTTGGCCAGACGTGAATCATGGATGACTATCCATGTTGATGCAGAAGAAATTGCAATGGAGGTGGAGAAAAATGTTTTGGAAACACTGGTTTTGGAAATCGTGTCTGAGATGACTATATCATAG
Protein sequence

>Msa0571600

MDRRSPPLVNQKCEWRMFGIFNLREAHSDGRSVSNSRQLDKHTSGNGKSRIGSDVLSTVDEKYPDVDVCSRRRRGYSCKSVRVENDRIADLENEVTKMIVNQRFFNKNSQGKDGADCQPNQFLDAVQVLYSNKELFTKLLQDPNSLLVKQIHDIQKTQVKAEQNGVIRLNKAQNSSSFDRFNSSSNCESQSSKKIVVSKPDTNNAQHFADTSSGNAQNIKPSNFTFGEIKRKLRHVMRVRRKEQQCGTTDSAPRKFPSSSQDLEDGKNVKKLDTSERNSPINVHVSSEKSLKFFKLKDSESTMRQEEVASEQNKLTVKDQGVKVLSHKKQQMVLKALHRDGESCSYSSSSAQKIKDLHVATFSNELQVFGAADISVNKSLSADNLHAHYDIPRETDGSLVQVDYDRFEENHLEDLITSSLDPMNKSKDIITEVLQTFSLKCDEPMKSHLSNLLMDSSSTFDEVNGLTDQLFDSTILHEYIIECFMELYQNSDFSPYFSSRNPNFQACVVRKVLVRQINELVKLHFFPHPSPITLQQLVERDLARRESWMTIHVDAEEIAMEVEKNVLETLVLEIVSEMTIS*