AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0580260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0580260 MtrunA17_Chr4g0039571 96.939 196 4 1 1 196 78 271 2.69e-133 374
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0580260 AT5G17070.2 52.761 163 59 4 2 158 51 201 8.65e-44 146
Msa0580260 AT5G17070.1 52.761 163 59 4 2 158 86 236 4.47e-43 145

Find 53 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCTATGCTCATCGGTTTA+TGG 0.222050 4_1:-85317890 None:intergenic
CTTGCCTTCTCTTGGTCATC+TGG 0.238861 4_1:-85317580 None:intergenic
CTCTGATCTGGTCAACAAAT+TGG 0.259921 4_1:+85316152 Msa0580260:CDS
TCGGTTTATGGATTTAAATA+TGG 0.278522 4_1:-85317878 None:intergenic
CAAACCCTTTGCAATGTAAA+TGG 0.297645 4_1:-85317137 None:intergenic
AACACTTTGTTCTCAGCATT+TGG 0.297719 4_1:-85317936 None:intergenic
CAATCTGATGGTGCACAAAA+TGG 0.303713 4_1:+85317625 Msa0580260:CDS
AAAGCAAGCTTGGAAAGATT+TGG 0.328975 4_1:-85317301 None:intergenic
TGGTCATCTGGTTCTTGTAC+TGG 0.331830 4_1:-85317568 None:intergenic
GCATTTCACTTGCCTTCTCT+TGG 0.337392 4_1:-85317588 None:intergenic
TATGTTAGTTGCTACTATAT+TGG 0.341025 4_1:+85318228 Msa0580260:three_prime_UTR
TTTCATAGTGTTTAATGCTC+TGG 0.379171 4_1:+85317968 Msa0580260:three_prime_UTR
TGTACTGGATCTTGTGGATA+AGG 0.381384 4_1:-85317553 None:intergenic
TGCTGAGAACAAAGTGTTAT+GGG 0.383768 4_1:+85317941 Msa0580260:three_prime_UTR
GCTTGTGCATTATTAGCATT+TGG 0.389227 4_1:-85317838 None:intergenic
ATGCTGAGAACAAAGTGTTA+TGG 0.389497 4_1:+85317940 Msa0580260:three_prime_UTR
TTCAAGAGCTAAAGCAAGCT+TGG 0.417322 4_1:-85317311 None:intergenic
GAGCAGCAATGTGCTTCATT+AGG 0.432277 4_1:+85316120 Msa0580260:CDS
TTGTATGGCACAGATTCTAT+TGG 0.438233 4_1:+85317261 Msa0580260:intron
TGATGATGTGGCCGTTGAAA+TGG 0.439402 4_1:+85317714 Msa0580260:CDS
TTAGTTGCTACTATATTGGT+TGG 0.446831 4_1:+85318232 Msa0580260:three_prime_UTR
ACCATTACTTCATCGGTGTC+TGG 0.448208 4_1:-85317676 None:intergenic
CAGCAAGAGCAGCAATCTGA+TGG 0.453930 4_1:+85317613 Msa0580260:CDS
TAGTGTTTAATGCTCTGGTC+TGG 0.470062 4_1:+85317973 Msa0580260:three_prime_UTR
AGGAGAGTCCTACTCTGATC+TGG 0.492552 4_1:+85316140 Msa0580260:CDS
CCCACCATTTACATTGCAAA+GGG 0.494748 4_1:+85317133 Msa0580260:CDS
TTCAAAAGTTTCCATTTCAA+CGG 0.494977 4_1:-85317725 None:intergenic
ACATCCAATAGATACAAAAC+AGG 0.502462 4_1:-85318045 None:intergenic
TATGGCACAGATTCTATTGG+AGG 0.505511 4_1:+85317264 Msa0580260:intron
GCTTGCTTTAGCTCTTGAAA+AGG 0.517580 4_1:+85317315 Msa0580260:CDS
CATAGTTGCTGCAGAGGGCA+TGG 0.520683 4_1:-85318209 None:intergenic
GCCCACCATTTACATTGCAA+AGG 0.522566 4_1:+85317132 Msa0580260:CDS
GGTTCTTGTACTGGATCTTG+TGG 0.524897 4_1:-85317559 None:intergenic
CCCTTTGCAATGTAAATGGT+GGG 0.531120 4_1:-85317133 None:intergenic
GCTGTCATTTCAGCTGAAGC+AGG 0.540223 4_1:+85315884 Msa0580260:CDS
CTGCAGAGGGCATGGCGACA+AGG 0.545643 4_1:-85318201 None:intergenic
CTGGTCAACAAATTGGATGA+AGG 0.546897 4_1:+85316159 Msa0580260:CDS
TGCATTATTAGCATTTGGCT+CGG 0.547032 4_1:-85317833 None:intergenic
TACATTGCAAAGGGTTTGTG+AGG 0.548155 4_1:+85317142 Msa0580260:CDS
TTTGTATAACAGGCATGACT+GGG 0.559350 4_1:+85315845 None:intergenic
ACCCTTTGCAATGTAAATGG+TGG 0.560756 4_1:-85317134 None:intergenic
CACCGATGAAGTAATGGTTG+AGG 0.562727 4_1:+85317681 Msa0580260:CDS
AGCCTCAACCATTACTTCAT+CGG 0.572202 4_1:-85317683 None:intergenic
TTTGTTGACCAGATCAGAGT+AGG 0.579578 4_1:-85316148 None:intergenic
AACTAACATAGTTGCTGCAG+AGG 0.590396 4_1:-85318215 None:intergenic
ACCAGACACCGATGAAGTAA+TGG 0.596618 4_1:+85317675 Msa0580260:CDS
ACATCTGAGTCTATGCTCAT+CGG 0.599091 4_1:-85317897 None:intergenic
GTGTCTGGTACAAGAGACTC+AGG 0.610539 4_1:-85317661 None:intergenic
TGACATGAGCTTGTATAACT+CGG 0.639653 4_1:+85318082 Msa0580260:three_prime_UTR
ACTAACATAGTTGCTGCAGA+GGG 0.642791 4_1:-85318214 None:intergenic
AGAACCAGATGACCAAGAGA+AGG 0.653708 4_1:+85317576 Msa0580260:CDS
GGCTGATACGAGTGATGATG+TGG 0.661091 4_1:+85317702 Msa0580260:CDS
GGTATTGTCCGAGTACCCTG+AGG 0.745796 4_1:+85316083 Msa0580260:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAAATATTTAATTACTCT+TGG + chr4_1:85316319-85316338 Msa0580260:intron 15.0%
!!! AAGAGCTAATAAAATTATTT+TGG + chr4_1:85316343-85316362 Msa0580260:intron 15.0%
!!! AGAGCTAATAAAATTATTTT+GGG + chr4_1:85316344-85316363 Msa0580260:intron 15.0%
!!! AAAATAGTTTATTTTTGTGT+TGG + chr4_1:85316716-85316735 Msa0580260:intron 15.0%
!!! TCAATTTTTTGATCAATATT+TGG - chr4_1:85317811-85317830 None:intergenic 15.0%
!! GTAAAATATTTAATTACTCT+TGG + chr4_1:85316319-85316338 Msa0580260:intron 15.0%
!!! AAGAGCTAATAAAATTATTT+TGG + chr4_1:85316343-85316362 Msa0580260:intron 15.0%
!!! AGAGCTAATAAAATTATTTT+GGG + chr4_1:85316344-85316363 Msa0580260:intron 15.0%
!!! AAAATAGTTTATTTTTGTGT+TGG + chr4_1:85316716-85316735 Msa0580260:intron 15.0%
!!! TCAATTTTTTGATCAATATT+TGG - chr4_1:85317811-85317830 None:intergenic 15.0%
!!! TATATTATGTTAGTTCTTAG+AGG + chr4_1:85315939-85315958 Msa0580260:intron 20.0%
!!! TTGTTCACATATTAGTTTAT+TGG + chr4_1:85315990-85316009 Msa0580260:intron 20.0%
!! TTGATGTCTATGAAATTTAA+TGG - chr4_1:85316220-85316239 None:intergenic 20.0%
!!! AAAAGCATTTCAAAGTTAAT+TGG - chr4_1:85317080-85317099 None:intergenic 20.0%
!!! ATGTTATCTGATATTTTGTA+TGG + chr4_1:85317246-85317265 Msa0580260:intron 20.0%
!!! TCTATTTTTTGATCAAGATT+TGG - chr4_1:85317781-85317800 None:intergenic 20.0%
!!! TATATTATGTTAGTTCTTAG+AGG + chr4_1:85315939-85315958 Msa0580260:intron 20.0%
!!! TTGTTCACATATTAGTTTAT+TGG + chr4_1:85315990-85316009 Msa0580260:intron 20.0%
!! TTGATGTCTATGAAATTTAA+TGG - chr4_1:85316220-85316239 None:intergenic 20.0%
!!! AAAAGCATTTCAAAGTTAAT+TGG - chr4_1:85317080-85317099 None:intergenic 20.0%
!!! ATGTTATCTGATATTTTGTA+TGG + chr4_1:85317246-85317265 Msa0580260:intron 20.0%
!!! TCTATTTTTTGATCAAGATT+TGG - chr4_1:85317781-85317800 None:intergenic 20.0%
! CAATACCTGTACAAAAATTA+TGG - chr4_1:85316070-85316089 None:intergenic 25.0%
! AATGGTACTCATAAAAAACA+GGG - chr4_1:85316202-85316221 None:intergenic 25.0%
! TAATGGTACTCATAAAAAAC+AGG - chr4_1:85316203-85316222 None:intergenic 25.0%
! CTCATTTAACTGATAAACAA+TGG + chr4_1:85316920-85316939 Msa0580260:intron 25.0%
! TTGAATAATGATCTCCTTTA+AGG + chr4_1:85317015-85317034 Msa0580260:intron 25.0%
! TGAATAATGATCTCCTTTAA+GGG + chr4_1:85317016-85317035 Msa0580260:intron 25.0%
! TAGTTCAGCAATAAATAACA+TGG - chr4_1:85317050-85317069 None:intergenic 25.0%
!!! TTAACTTTGAAATGCTTTTC+TGG + chr4_1:85317081-85317100 Msa0580260:intron 25.0%
! TGTTGTGCATTATTATATTC+TGG + chr4_1:85317184-85317203 Msa0580260:intron 25.0%
! GTTGTGCATTATTATATTCT+GGG + chr4_1:85317185-85317204 Msa0580260:intron 25.0%
! GATTAACCAATTACAAATAC+AGG - chr4_1:85317410-85317429 None:intergenic 25.0%
!! TGGTTAATCATGCATTTTTA+TGG + chr4_1:85317421-85317440 Msa0580260:intron 25.0%
! CACATGAATTATAGATAACT+TGG + chr4_1:85317448-85317467 Msa0580260:intron 25.0%
! TTCAAAAGTTTCCATTTCAA+CGG - chr4_1:85317728-85317747 None:intergenic 25.0%
!! TTTTTGATCAATATTTGGCT+CGG - chr4_1:85317806-85317825 None:intergenic 25.0%
! TCGGTTTATGGATTTAAATA+TGG - chr4_1:85317881-85317900 None:intergenic 25.0%
! TATGTTAGTTGCTACTATAT+TGG + chr4_1:85318228-85318247 Msa0580260:three_prime_UTR 25.0%
! CAATACCTGTACAAAAATTA+TGG - chr4_1:85316070-85316089 None:intergenic 25.0%
! AATGGTACTCATAAAAAACA+GGG - chr4_1:85316202-85316221 None:intergenic 25.0%
! TAATGGTACTCATAAAAAAC+AGG - chr4_1:85316203-85316222 None:intergenic 25.0%
! CTCATTTAACTGATAAACAA+TGG + chr4_1:85316920-85316939 Msa0580260:intron 25.0%
! TTGAATAATGATCTCCTTTA+AGG + chr4_1:85317015-85317034 Msa0580260:intron 25.0%
! TGAATAATGATCTCCTTTAA+GGG + chr4_1:85317016-85317035 Msa0580260:intron 25.0%
! TAGTTCAGCAATAAATAACA+TGG - chr4_1:85317050-85317069 None:intergenic 25.0%
!!! TTAACTTTGAAATGCTTTTC+TGG + chr4_1:85317081-85317100 Msa0580260:intron 25.0%
! TGTTGTGCATTATTATATTC+TGG + chr4_1:85317184-85317203 Msa0580260:intron 25.0%
! GTTGTGCATTATTATATTCT+GGG + chr4_1:85317185-85317204 Msa0580260:intron 25.0%
! GATTAACCAATTACAAATAC+AGG - chr4_1:85317410-85317429 None:intergenic 25.0%
!! TGGTTAATCATGCATTTTTA+TGG + chr4_1:85317421-85317440 Msa0580260:intron 25.0%
! CACATGAATTATAGATAACT+TGG + chr4_1:85317448-85317467 Msa0580260:intron 25.0%
! TTCAAAAGTTTCCATTTCAA+CGG - chr4_1:85317728-85317747 None:intergenic 25.0%
!! TTTTTGATCAATATTTGGCT+CGG - chr4_1:85317806-85317825 None:intergenic 25.0%
! TCGGTTTATGGATTTAAATA+TGG - chr4_1:85317881-85317900 None:intergenic 25.0%
! TATGTTAGTTGCTACTATAT+TGG + chr4_1:85318228-85318247 Msa0580260:three_prime_UTR 25.0%
! TCTCTCCATAATTTTTGTAC+AGG + chr4_1:85316062-85316081 Msa0580260:intron 30.0%
ATAAAAAACAGGGGAACTAA+AGG - chr4_1:85316192-85316211 None:intergenic 30.0%
ATGGTACTCATAAAAAACAG+GGG - chr4_1:85316201-85316220 None:intergenic 30.0%
GAATATCAATCTTAGAGATG+TGG + chr4_1:85316366-85316385 Msa0580260:intron 30.0%
TGGTACACTTATCAAAACAT+TGG + chr4_1:85316539-85316558 Msa0580260:intron 30.0%
AATTTCGAAACTAGAATGGA+TGG - chr4_1:85316862-85316881 None:intergenic 30.0%
CTCTGTTCTTTCTTAATCAT+TGG + chr4_1:85316977-85316996 Msa0580260:intron 30.0%
!! TTATTCAAGAGATTTGTTGC+AGG - chr4_1:85317003-85317022 None:intergenic 30.0%
TCAATAAAACATGTAAGTGC+TGG - chr4_1:85317110-85317129 None:intergenic 30.0%
!! GCACTTACATGTTTTATTGA+TGG + chr4_1:85317110-85317129 Msa0580260:intron 30.0%
! ATGTTAACCTTGATGGATAT+TGG - chr4_1:85317212-85317231 None:intergenic 30.0%
TACTATCATGTTAACCTTGA+TGG - chr4_1:85317219-85317238 None:intergenic 30.0%
GAAGATCCTGTATTTGTAAT+TGG + chr4_1:85317401-85317420 Msa0580260:intron 30.0%
AAAAACACCATATAGAAGTC+AGG - chr4_1:85317486-85317505 None:intergenic 30.0%
TTTCATAGTGTTTAATGCTC+TGG + chr4_1:85317968-85317987 Msa0580260:three_prime_UTR 30.0%
! CTTTCCTGTTTTGTATCTAT+TGG + chr4_1:85318041-85318060 Msa0580260:three_prime_UTR 30.0%
ACATCCAATAGATACAAAAC+AGG - chr4_1:85318048-85318067 None:intergenic 30.0%
TTAGTTGCTACTATATTGGT+TGG + chr4_1:85318232-85318251 Msa0580260:three_prime_UTR 30.0%
! TCTCTCCATAATTTTTGTAC+AGG + chr4_1:85316062-85316081 Msa0580260:intron 30.0%
ATAAAAAACAGGGGAACTAA+AGG - chr4_1:85316192-85316211 None:intergenic 30.0%
ATGGTACTCATAAAAAACAG+GGG - chr4_1:85316201-85316220 None:intergenic 30.0%
GAATATCAATCTTAGAGATG+TGG + chr4_1:85316366-85316385 Msa0580260:intron 30.0%
TGGTACACTTATCAAAACAT+TGG + chr4_1:85316539-85316558 Msa0580260:intron 30.0%
AATTTCGAAACTAGAATGGA+TGG - chr4_1:85316862-85316881 None:intergenic 30.0%
CTCTGTTCTTTCTTAATCAT+TGG + chr4_1:85316977-85316996 Msa0580260:intron 30.0%
!! TTATTCAAGAGATTTGTTGC+AGG - chr4_1:85317003-85317022 None:intergenic 30.0%
TCAATAAAACATGTAAGTGC+TGG - chr4_1:85317110-85317129 None:intergenic 30.0%
!! GCACTTACATGTTTTATTGA+TGG + chr4_1:85317110-85317129 Msa0580260:intron 30.0%
! ATGTTAACCTTGATGGATAT+TGG - chr4_1:85317212-85317231 None:intergenic 30.0%
TACTATCATGTTAACCTTGA+TGG - chr4_1:85317219-85317238 None:intergenic 30.0%
GAAGATCCTGTATTTGTAAT+TGG + chr4_1:85317401-85317420 Msa0580260:intron 30.0%
AAAAACACCATATAGAAGTC+AGG - chr4_1:85317486-85317505 None:intergenic 30.0%
TTTCATAGTGTTTAATGCTC+TGG + chr4_1:85317968-85317987 Msa0580260:three_prime_UTR 30.0%
! CTTTCCTGTTTTGTATCTAT+TGG + chr4_1:85318041-85318060 Msa0580260:three_prime_UTR 30.0%
ACATCCAATAGATACAAAAC+AGG - chr4_1:85318048-85318067 None:intergenic 30.0%
TTAGTTGCTACTATATTGGT+TGG + chr4_1:85318232-85318251 Msa0580260:three_prime_UTR 30.0%
!! TTTTCCCTTGGTGCATTTAA+TGG + chr4_1:85316409-85316428 Msa0580260:intron 35.0%
ACAACCATTAAATGCACCAA+GGG - chr4_1:85316416-85316435 None:intergenic 35.0%
TACAACCATTAAATGCACCA+AGG - chr4_1:85316417-85316436 None:intergenic 35.0%
!!! ACTTGTTTTAGAGTCTTGAC+TGG + chr4_1:85316485-85316504 Msa0580260:intron 35.0%
AGCGAATTTCGAAACTAGAA+TGG - chr4_1:85316866-85316885 None:intergenic 35.0%
! TGAGTGTCACTACTTCATTA+TGG - chr4_1:85316904-85316923 None:intergenic 35.0%
CAAACCCTTTGCAATGTAAA+TGG - chr4_1:85317140-85317159 None:intergenic 35.0%
TTGTATGGCACAGATTCTAT+TGG + chr4_1:85317261-85317280 Msa0580260:intron 35.0%
AAAGCAAGCTTGGAAAGATT+TGG - chr4_1:85317304-85317323 None:intergenic 35.0%
! CATTTTGCCTGACTTCTATA+TGG + chr4_1:85317476-85317495 Msa0580260:intron 35.0%
TGCATTATTAGCATTTGGCT+CGG - chr4_1:85317836-85317855 None:intergenic 35.0%
GCTTGTGCATTATTAGCATT+TGG - chr4_1:85317841-85317860 None:intergenic 35.0%
AACACTTTGTTCTCAGCATT+TGG - chr4_1:85317939-85317958 None:intergenic 35.0%
! ATGCTGAGAACAAAGTGTTA+TGG + chr4_1:85317940-85317959 Msa0580260:three_prime_UTR 35.0%
! TGCTGAGAACAAAGTGTTAT+GGG + chr4_1:85317941-85317960 Msa0580260:three_prime_UTR 35.0%
TGACATGAGCTTGTATAACT+CGG + chr4_1:85318082-85318101 Msa0580260:three_prime_UTR 35.0%
!!! GTTGGTTTTTGCGATTATTG+AGG + chr4_1:85318250-85318269 Msa0580260:three_prime_UTR 35.0%
!! TTTTCCCTTGGTGCATTTAA+TGG + chr4_1:85316409-85316428 Msa0580260:intron 35.0%
ACAACCATTAAATGCACCAA+GGG - chr4_1:85316416-85316435 None:intergenic 35.0%
TACAACCATTAAATGCACCA+AGG - chr4_1:85316417-85316436 None:intergenic 35.0%
!!! ACTTGTTTTAGAGTCTTGAC+TGG + chr4_1:85316485-85316504 Msa0580260:intron 35.0%
AGCGAATTTCGAAACTAGAA+TGG - chr4_1:85316866-85316885 None:intergenic 35.0%
! TGAGTGTCACTACTTCATTA+TGG - chr4_1:85316904-85316923 None:intergenic 35.0%
CAAACCCTTTGCAATGTAAA+TGG - chr4_1:85317140-85317159 None:intergenic 35.0%
TTGTATGGCACAGATTCTAT+TGG + chr4_1:85317261-85317280 Msa0580260:intron 35.0%
AAAGCAAGCTTGGAAAGATT+TGG - chr4_1:85317304-85317323 None:intergenic 35.0%
! CATTTTGCCTGACTTCTATA+TGG + chr4_1:85317476-85317495 Msa0580260:intron 35.0%
TGCATTATTAGCATTTGGCT+CGG - chr4_1:85317836-85317855 None:intergenic 35.0%
GCTTGTGCATTATTAGCATT+TGG - chr4_1:85317841-85317860 None:intergenic 35.0%
AACACTTTGTTCTCAGCATT+TGG - chr4_1:85317939-85317958 None:intergenic 35.0%
! ATGCTGAGAACAAAGTGTTA+TGG + chr4_1:85317940-85317959 Msa0580260:three_prime_UTR 35.0%
! TGCTGAGAACAAAGTGTTAT+GGG + chr4_1:85317941-85317960 Msa0580260:three_prime_UTR 35.0%
TGACATGAGCTTGTATAACT+CGG + chr4_1:85318082-85318101 Msa0580260:three_prime_UTR 35.0%
!!! GTTGGTTTTTGCGATTATTG+AGG + chr4_1:85318250-85318269 Msa0580260:three_prime_UTR 35.0%
ATTTCAGCTGAAGCAGGTAT+GGG + chr4_1:85315890-85315909 Msa0580260:intron 40.0%
! CACTTGTCAATTTTGCCTCA+GGG - chr4_1:85316101-85316120 None:intergenic 40.0%
! TCACTTGTCAATTTTGCCTC+AGG - chr4_1:85316102-85316121 None:intergenic 40.0%
TTTGTTGACCAGATCAGAGT+AGG - chr4_1:85316151-85316170 None:intergenic 40.0%
CTCTGATCTGGTCAACAAAT+TGG + chr4_1:85316152-85316171 Msa0580260:CDS 40.0%
CTGGTCAACAAATTGGATGA+AGG + chr4_1:85316159-85316178 Msa0580260:CDS 40.0%
AAAACAGGGGAACTAAAGGT+GGG - chr4_1:85316188-85316207 None:intergenic 40.0%
AAAAACAGGGGAACTAAAGG+TGG - chr4_1:85316189-85316208 None:intergenic 40.0%
GGAAAAGTGAGTACCAAACA+CGG - chr4_1:85316395-85316414 None:intergenic 40.0%
!! TTTGGTACTCACTTTTCCCT+TGG + chr4_1:85316397-85316416 Msa0580260:intron 40.0%
!! GTTTTGATAAGTGTACCAGC+AGG - chr4_1:85316537-85316556 None:intergenic 40.0%
AACATTGGCCATATAGCTAC+TGG + chr4_1:85316554-85316573 Msa0580260:intron 40.0%
TCCAGTCTAGACATACATGT+TGG - chr4_1:85316742-85316761 None:intergenic 40.0%
GGATACTCTTCTACAAGCTT+TGG - chr4_1:85316786-85316805 None:intergenic 40.0%
TCCATGAGAGATAAACTTCC+TGG + chr4_1:85316803-85316822 Msa0580260:intron 40.0%
GAAAGAACAGAGTCTGAGTT+GGG - chr4_1:85316969-85316988 None:intergenic 40.0%
AGAAAGAACAGAGTCTGAGT+TGG - chr4_1:85316970-85316989 None:intergenic 40.0%
CCCTTTGCAATGTAAATGGT+GGG - chr4_1:85317136-85317155 None:intergenic 40.0%
CCCACCATTTACATTGCAAA+GGG + chr4_1:85317133-85317152 Msa0580260:CDS 40.0%
ACCCTTTGCAATGTAAATGG+TGG - chr4_1:85317137-85317156 None:intergenic 40.0%
TACATTGCAAAGGGTTTGTG+AGG + chr4_1:85317142-85317161 Msa0580260:CDS 40.0%
TATGGCACAGATTCTATTGG+AGG + chr4_1:85317264-85317283 Msa0580260:intron 40.0%
TTCAAGAGCTAAAGCAAGCT+TGG - chr4_1:85317314-85317333 None:intergenic 40.0%
!! GCTTGCTTTAGCTCTTGAAA+AGG + chr4_1:85317315-85317334 Msa0580260:CDS 40.0%
TGTACTGGATCTTGTGGATA+AGG - chr4_1:85317556-85317575 None:intergenic 40.0%
! CAATCTGATGGTGCACAAAA+TGG + chr4_1:85317625-85317644 Msa0580260:CDS 40.0%
AGCCTCAACCATTACTTCAT+CGG - chr4_1:85317686-85317705 None:intergenic 40.0%
!! AGTCTATGCTCATCGGTTTA+TGG - chr4_1:85317893-85317912 None:intergenic 40.0%
ACATCTGAGTCTATGCTCAT+CGG - chr4_1:85317900-85317919 None:intergenic 40.0%
TAGTGTTTAATGCTCTGGTC+TGG + chr4_1:85317973-85317992 Msa0580260:three_prime_UTR 40.0%
ACTAACATAGTTGCTGCAGA+GGG - chr4_1:85318217-85318236 None:intergenic 40.0%
AACTAACATAGTTGCTGCAG+AGG - chr4_1:85318218-85318237 None:intergenic 40.0%
ATTTCAGCTGAAGCAGGTAT+GGG + chr4_1:85315890-85315909 Msa0580260:intron 40.0%
! CACTTGTCAATTTTGCCTCA+GGG - chr4_1:85316101-85316120 None:intergenic 40.0%
! TCACTTGTCAATTTTGCCTC+AGG - chr4_1:85316102-85316121 None:intergenic 40.0%
TTTGTTGACCAGATCAGAGT+AGG - chr4_1:85316151-85316170 None:intergenic 40.0%
CTCTGATCTGGTCAACAAAT+TGG + chr4_1:85316152-85316171 Msa0580260:CDS 40.0%
CTGGTCAACAAATTGGATGA+AGG + chr4_1:85316159-85316178 Msa0580260:CDS 40.0%
AAAACAGGGGAACTAAAGGT+GGG - chr4_1:85316188-85316207 None:intergenic 40.0%
AAAAACAGGGGAACTAAAGG+TGG - chr4_1:85316189-85316208 None:intergenic 40.0%
GGAAAAGTGAGTACCAAACA+CGG - chr4_1:85316395-85316414 None:intergenic 40.0%
!! TTTGGTACTCACTTTTCCCT+TGG + chr4_1:85316397-85316416 Msa0580260:intron 40.0%
!! GTTTTGATAAGTGTACCAGC+AGG - chr4_1:85316537-85316556 None:intergenic 40.0%
AACATTGGCCATATAGCTAC+TGG + chr4_1:85316554-85316573 Msa0580260:intron 40.0%
TCCAGTCTAGACATACATGT+TGG - chr4_1:85316742-85316761 None:intergenic 40.0%
GGATACTCTTCTACAAGCTT+TGG - chr4_1:85316786-85316805 None:intergenic 40.0%
TCCATGAGAGATAAACTTCC+TGG + chr4_1:85316803-85316822 Msa0580260:intron 40.0%
GAAAGAACAGAGTCTGAGTT+GGG - chr4_1:85316969-85316988 None:intergenic 40.0%
AGAAAGAACAGAGTCTGAGT+TGG - chr4_1:85316970-85316989 None:intergenic 40.0%
CCCTTTGCAATGTAAATGGT+GGG - chr4_1:85317136-85317155 None:intergenic 40.0%
CCCACCATTTACATTGCAAA+GGG + chr4_1:85317133-85317152 Msa0580260:CDS 40.0%
ACCCTTTGCAATGTAAATGG+TGG - chr4_1:85317137-85317156 None:intergenic 40.0%
TACATTGCAAAGGGTTTGTG+AGG + chr4_1:85317142-85317161 Msa0580260:CDS 40.0%
TATGGCACAGATTCTATTGG+AGG + chr4_1:85317264-85317283 Msa0580260:intron 40.0%
TTCAAGAGCTAAAGCAAGCT+TGG - chr4_1:85317314-85317333 None:intergenic 40.0%
!! GCTTGCTTTAGCTCTTGAAA+AGG + chr4_1:85317315-85317334 Msa0580260:CDS 40.0%
TGTACTGGATCTTGTGGATA+AGG - chr4_1:85317556-85317575 None:intergenic 40.0%
! CAATCTGATGGTGCACAAAA+TGG + chr4_1:85317625-85317644 Msa0580260:CDS 40.0%
AGCCTCAACCATTACTTCAT+CGG - chr4_1:85317686-85317705 None:intergenic 40.0%
!! AGTCTATGCTCATCGGTTTA+TGG - chr4_1:85317893-85317912 None:intergenic 40.0%
ACATCTGAGTCTATGCTCAT+CGG - chr4_1:85317900-85317919 None:intergenic 40.0%
TAGTGTTTAATGCTCTGGTC+TGG + chr4_1:85317973-85317992 Msa0580260:three_prime_UTR 40.0%
ACTAACATAGTTGCTGCAGA+GGG - chr4_1:85318217-85318236 None:intergenic 40.0%
AACTAACATAGTTGCTGCAG+AGG - chr4_1:85318218-85318237 None:intergenic 40.0%
CATTTCAGCTGAAGCAGGTA+TGG + chr4_1:85315889-85315908 Msa0580260:intron 45.0%
TTTCAGCTGAAGCAGGTATG+GGG + chr4_1:85315891-85315910 Msa0580260:intron 45.0%
!! CAATTTTGCCTCAGGGTACT+CGG - chr4_1:85316094-85316113 None:intergenic 45.0%
GAGCAGCAATGTGCTTCATT+AGG + chr4_1:85316120-85316139 Msa0580260:CDS 45.0%
AGAGATGTGGAATCCGTGTT+TGG + chr4_1:85316379-85316398 Msa0580260:intron 45.0%
GCTACACACCAGTAGCTATA+TGG - chr4_1:85316565-85316584 None:intergenic 45.0%
GCCAACATGTATGTCTAGAC+TGG + chr4_1:85316738-85316757 Msa0580260:intron 45.0%
GCCAGGAAGTTTATCTCTCA+TGG - chr4_1:85316807-85316826 None:intergenic 45.0%
CATGGCATATGCACCCTTAA+AGG - chr4_1:85317032-85317051 None:intergenic 45.0%
GCCCACCATTTACATTGCAA+AGG + chr4_1:85317132-85317151 Msa0580260:CDS 45.0%
TCTGGGTCCAATATCCATCA+AGG + chr4_1:85317202-85317221 Msa0580260:intron 45.0%
GGTTCTTGTACTGGATCTTG+TGG - chr4_1:85317562-85317581 None:intergenic 45.0%
!! TGGTCATCTGGTTCTTGTAC+TGG - chr4_1:85317571-85317590 None:intergenic 45.0%
AGAACCAGATGACCAAGAGA+AGG + chr4_1:85317576-85317595 Msa0580260:CDS 45.0%
GCATTTCACTTGCCTTCTCT+TGG - chr4_1:85317591-85317610 None:intergenic 45.0%
ACCAGACACCGATGAAGTAA+TGG + chr4_1:85317675-85317694 Msa0580260:CDS 45.0%
! ACCATTACTTCATCGGTGTC+TGG - chr4_1:85317679-85317698 None:intergenic 45.0%
CACCGATGAAGTAATGGTTG+AGG + chr4_1:85317681-85317700 Msa0580260:CDS 45.0%
! TGATGATGTGGCCGTTGAAA+TGG + chr4_1:85317714-85317733 Msa0580260:CDS 45.0%
CATTTCAGCTGAAGCAGGTA+TGG + chr4_1:85315889-85315908 Msa0580260:intron 45.0%
TTTCAGCTGAAGCAGGTATG+GGG + chr4_1:85315891-85315910 Msa0580260:intron 45.0%
!! CAATTTTGCCTCAGGGTACT+CGG - chr4_1:85316094-85316113 None:intergenic 45.0%
GAGCAGCAATGTGCTTCATT+AGG + chr4_1:85316120-85316139 Msa0580260:CDS 45.0%
AGAGATGTGGAATCCGTGTT+TGG + chr4_1:85316379-85316398 Msa0580260:intron 45.0%
GCTACACACCAGTAGCTATA+TGG - chr4_1:85316565-85316584 None:intergenic 45.0%
GCCAACATGTATGTCTAGAC+TGG + chr4_1:85316738-85316757 Msa0580260:intron 45.0%
GCCAGGAAGTTTATCTCTCA+TGG - chr4_1:85316807-85316826 None:intergenic 45.0%
CATGGCATATGCACCCTTAA+AGG - chr4_1:85317032-85317051 None:intergenic 45.0%
GCCCACCATTTACATTGCAA+AGG + chr4_1:85317132-85317151 Msa0580260:CDS 45.0%
TCTGGGTCCAATATCCATCA+AGG + chr4_1:85317202-85317221 Msa0580260:intron 45.0%
GGTTCTTGTACTGGATCTTG+TGG - chr4_1:85317562-85317581 None:intergenic 45.0%
!! TGGTCATCTGGTTCTTGTAC+TGG - chr4_1:85317571-85317590 None:intergenic 45.0%
AGAACCAGATGACCAAGAGA+AGG + chr4_1:85317576-85317595 Msa0580260:CDS 45.0%
GCATTTCACTTGCCTTCTCT+TGG - chr4_1:85317591-85317610 None:intergenic 45.0%
ACCAGACACCGATGAAGTAA+TGG + chr4_1:85317675-85317694 Msa0580260:CDS 45.0%
! ACCATTACTTCATCGGTGTC+TGG - chr4_1:85317679-85317698 None:intergenic 45.0%
CACCGATGAAGTAATGGTTG+AGG + chr4_1:85317681-85317700 Msa0580260:CDS 45.0%
! TGATGATGTGGCCGTTGAAA+TGG + chr4_1:85317714-85317733 Msa0580260:CDS 45.0%
! GCTGTCATTTCAGCTGAAGC+AGG + chr4_1:85315884-85315903 Msa0580260:CDS 50.0%
AGGAGAGTCCTACTCTGATC+TGG + chr4_1:85316140-85316159 Msa0580260:CDS 50.0%
GGAGCAGTTCACAGTTGAAC+TGG + chr4_1:85316506-85316525 Msa0580260:intron 50.0%
CACAACACATGCTAATCGCC+AGG - chr4_1:85316824-85316843 None:intergenic 50.0%
CTTGCCTTCTCTTGGTCATC+TGG - chr4_1:85317583-85317602 None:intergenic 50.0%
CAGCAAGAGCAGCAATCTGA+TGG + chr4_1:85317613-85317632 Msa0580260:CDS 50.0%
GTGTCTGGTACAAGAGACTC+AGG - chr4_1:85317664-85317683 None:intergenic 50.0%
! GGCTGATACGAGTGATGATG+TGG + chr4_1:85317702-85317721 Msa0580260:CDS 50.0%
! GCTGTCATTTCAGCTGAAGC+AGG + chr4_1:85315884-85315903 Msa0580260:CDS 50.0%
AGGAGAGTCCTACTCTGATC+TGG + chr4_1:85316140-85316159 Msa0580260:CDS 50.0%
GGAGCAGTTCACAGTTGAAC+TGG + chr4_1:85316506-85316525 Msa0580260:intron 50.0%
CACAACACATGCTAATCGCC+AGG - chr4_1:85316824-85316843 None:intergenic 50.0%
CTTGCCTTCTCTTGGTCATC+TGG - chr4_1:85317583-85317602 None:intergenic 50.0%
CAGCAAGAGCAGCAATCTGA+TGG + chr4_1:85317613-85317632 Msa0580260:CDS 50.0%
GTGTCTGGTACAAGAGACTC+AGG - chr4_1:85317664-85317683 None:intergenic 50.0%
! GGCTGATACGAGTGATGATG+TGG + chr4_1:85317702-85317721 Msa0580260:CDS 50.0%
GGTATTGTCCGAGTACCCTG+AGG + chr4_1:85316083-85316102 Msa0580260:intron 55.0%
GTTGAACTGGACTGACCTGC+TGG + chr4_1:85316519-85316538 Msa0580260:intron 55.0%
CATAGTTGCTGCAGAGGGCA+TGG - chr4_1:85318212-85318231 None:intergenic 55.0%
GGTATTGTCCGAGTACCCTG+AGG + chr4_1:85316083-85316102 Msa0580260:intron 55.0%
GTTGAACTGGACTGACCTGC+TGG + chr4_1:85316519-85316538 Msa0580260:intron 55.0%
CATAGTTGCTGCAGAGGGCA+TGG - chr4_1:85318212-85318231 None:intergenic 55.0%
CAGCTGAAGCAGGTATGGGG+AGG + chr4_1:85315894-85315913 Msa0580260:intron 60.0%
CAGCTGAAGCAGGTATGGGG+AGG + chr4_1:85315894-85315913 Msa0580260:intron 60.0%
! CTGCAGAGGGCATGGCGACA+AGG - chr4_1:85318204-85318223 None:intergenic 65.0%
! CTGCAGAGGGCATGGCGACA+AGG - chr4_1:85318204-85318223 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 85315857 85318274 85315857 ID=Msa0580260;Name=Msa0580260
chr4_1 mRNA 85315857 85318274 85315857 ID=Msa0580260-mRNA-1;Parent=Msa0580260;Name=Msa0580260-mRNA-1;_AED=0.26;_eAED=0.26;_QI=25|1|0.8|1|1|1|5|407|196
chr4_1 exon 85315857 85315905 85315857 ID=Msa0580260-mRNA-1:exon:31296;Parent=Msa0580260-mRNA-1
chr4_1 exon 85316084 85316180 85316084 ID=Msa0580260-mRNA-1:exon:31297;Parent=Msa0580260-mRNA-1
chr4_1 exon 85317111 85317163 85317111 ID=Msa0580260-mRNA-1:exon:31298;Parent=Msa0580260-mRNA-1
chr4_1 exon 85317274 85317336 85317274 ID=Msa0580260-mRNA-1:exon:31299;Parent=Msa0580260-mRNA-1
chr4_1 exon 85317514 85318274 85317514 ID=Msa0580260-mRNA-1:exon:31300;Parent=Msa0580260-mRNA-1
chr4_1 five_prime_UTR 85315857 85315881 85315857 ID=Msa0580260-mRNA-1:five_prime_utr;Parent=Msa0580260-mRNA-1
chr4_1 CDS 85315882 85315905 85315882 ID=Msa0580260-mRNA-1:cds;Parent=Msa0580260-mRNA-1
chr4_1 CDS 85316084 85316180 85316084 ID=Msa0580260-mRNA-1:cds;Parent=Msa0580260-mRNA-1
chr4_1 CDS 85317111 85317163 85317111 ID=Msa0580260-mRNA-1:cds;Parent=Msa0580260-mRNA-1
chr4_1 CDS 85317274 85317336 85317274 ID=Msa0580260-mRNA-1:cds;Parent=Msa0580260-mRNA-1
chr4_1 CDS 85317514 85317867 85317514 ID=Msa0580260-mRNA-1:cds;Parent=Msa0580260-mRNA-1
chr4_1 three_prime_UTR 85317868 85318274 85317868 ID=Msa0580260-mRNA-1:three_prime_utr;Parent=Msa0580260-mRNA-1
Gene Sequence

>Msa0580260

ATGCTGTCATTTCAGCTGAAGCAGGTATTGTCCGAGTACCCTGAGGCAAAATTGACAAGTGAGCAGCAATGTGCTTCATTAGGAGAGTCCTACTCTGATCTGGTCAACAAATTGGATGAAGCACTTACATGTTTTATTGATGGCCCACCATTTACATTGCAAAGGGTTTGTGAGATTCTATTGGAGGCAAAAAACATTTATCCAAATCTTTCCAAGCTTGCTTTAGCTCTTGAAAAGAACTTGTTAGTTACATCTACATTGACAATTTGCACTGATCCTTATCCACAAGATCCAGTACAAGAACCAGATGACCAAGAGAAGGCAAGTGAAATGCAGCAGCAAGAGCAGCAATCTGATGGTGCACAAAATGGCGCTGAGACTGAGCCTGAGTCTCTTGTACCAGACACCGATGAAGTAATGGTTGAGGCTGATACGAGTGATGATGTGGCCGTTGAAATGGAAACTTTTGAAGATGCTAAATCATCAGAATTAGATTCTGAGCCAAATCTTGATCAAAAAATAGATTCCGAGCCAAATATTGATCAAAAAATTGATTCCGAGCCAAATGCTAATAATGCACAAGCTTCTTGA
Protein sequence

>Msa0580260

MLSFQLKQVLSEYPEAKLTSEQQCASLGESYSDLVNKLDEALTCFIDGPPFTLQRVCEILLEAKNIYPNLSKLALALEKNLLVTSTLTICTDPYPQDPVQEPDDQEKASEMQQQEQQSDGAQNGAETEPESLVPDTDEVMVEADTSDDVAVEMETFEDAKSSELDSEPNLDQKIDSEPNIDQKIDSEPNANNAQAS*