AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0581210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0581210 MtrunA17_Chr7g0269081 96.405 306 9 2 1 304 1 306 0.0 591
Msa0581210 MtrunA17_Chr4g0067291 35.016 317 146 14 19 304 71 358 1.28e-34 129
Msa0581210 MtrunA17_Chr1g0208301 67.606 71 23 0 16 86 7 77 6.06e-26 105
Msa0581210 MtrunA17_Chr2g0287751 67.143 70 23 0 17 86 5 74 4.77e-24 101
Msa0581210 MtrunA17_Chr4g0065651 65.714 70 24 0 17 86 5 74 5.53e-23 98.6
Msa0581210 MtrunA17_Chr0c22g0493881 65.714 70 24 0 17 86 5 74 6.20e-23 97.4
Msa0581210 MtrunA17_Chr4g0065621 65.714 70 24 0 17 86 5 74 5.72e-22 94.7
Msa0581210 MtrunA17_Chr2g0296001 45.385 130 62 2 2 130 61 182 1.30e-21 93.6
Msa0581210 MtrunA17_Chr7g0272571 47.899 119 58 2 22 140 33 147 3.39e-21 92.4
Msa0581210 MtrunA17_Chr1g0192131 53.933 89 33 2 1 88 1 82 9.39e-21 91.7
Msa0581210 MtrunA17_Chr1g0191671 57.143 70 30 0 17 86 5 74 2.75e-19 88.2
Msa0581210 MtrunA17_Chr3g0111541 48.611 72 37 0 17 88 7 78 2.10e-15 73.6
Msa0581210 MtrunA17_Chr2g0287741 61.905 63 24 0 24 86 17 79 5.79e-15 75.1
Msa0581210 MtrunA17_Chr4g0036931 44.444 72 39 1 20 90 26 97 5.05e-14 68.6
Msa0581210 MtrunA17_Chr1g0160201 43.478 69 39 0 22 90 25 93 5.66e-14 68.2
Msa0581210 MtrunA17_Chr3g0124411 47.887 71 37 0 20 90 28 98 9.42e-14 67.8
Msa0581210 MtrunA17_Chr5g0407821 42.424 66 38 0 22 87 23 88 3.69e-13 65.9
Msa0581210 MtrunA17_Chr8g0382721 47.692 65 34 0 26 90 33 97 8.62e-13 65.1
Msa0581210 MtrunA17_Chr7g0218981 40.000 70 42 0 19 88 1 70 1.96e-12 63.2
Msa0581210 MtrunA17_Chr3g0115421 33.010 103 56 2 26 115 30 132 8.06e-12 62.4
Msa0581210 MtrunA17_Chr3g0145621 37.500 72 44 1 20 90 24 95 1.87e-11 61.2
Msa0581210 MtrunA17_Chr1g0198341 40.260 77 45 1 19 94 1 77 2.11e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0581210 AT3G06130.2 65.714 70 24 0 17 86 5 74 1.08e-23 99.8
Msa0581210 AT3G06130.1 65.714 70 24 0 17 86 5 74 1.41e-23 100
Msa0581210 AT5G27690.1 59.155 71 29 0 17 87 25 95 3.26e-23 98.2
Msa0581210 AT5G19090.2 64.286 70 25 0 17 86 5 74 1.52e-22 97.4
Msa0581210 AT5G19090.3 64.286 70 25 0 17 86 5 74 1.52e-22 97.4
Msa0581210 AT5G19090.5 64.286 70 25 0 17 86 5 74 2.41e-22 97.4
Msa0581210 AT5G19090.4 64.286 70 25 0 17 86 5 74 2.41e-22 97.4
Msa0581210 AT5G19090.1 64.286 70 25 0 17 86 5 74 2.41e-22 97.4
Msa0581210 AT1G23000.1 45.455 99 46 1 16 106 7 105 4.86e-21 92.4
Msa0581210 AT1G23000.2 45.455 99 46 1 16 106 67 165 1.28e-20 91.7
Msa0581210 AT1G56210.1 54.286 70 32 0 21 90 39 108 2.00e-19 87.8
Msa0581210 AT3G05220.1 55.714 70 31 0 17 86 5 74 2.83e-18 85.5
Msa0581210 AT5G37860.1 48.611 72 37 0 22 93 10 81 2.51e-15 74.7
Msa0581210 AT1G71050.1 42.029 69 40 0 22 90 28 96 9.84e-13 65.1
Msa0581210 AT1G06330.1 45.902 61 33 0 26 86 6 66 4.85e-12 63.2
Msa0581210 AT4G38580.1 40.278 72 42 1 20 90 24 95 1.98e-11 61.6
Msa0581210 AT4G38580.2 40.278 72 42 1 20 90 24 95 1.98e-11 61.6
Msa0581210 AT3G56240.3 43.939 66 37 0 22 87 3 68 2.11e-11 60.8
Msa0581210 AT3G56240.1 43.939 66 37 0 22 87 3 68 2.11e-11 60.8
Msa0581210 AT3G56891.1 43.548 62 35 0 26 87 20 81 3.42e-11 61.2
Msa0581210 AT3G56891.2 43.103 58 33 0 30 87 1 58 3.62e-11 60.5

Find 104 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCACAAGGTTACAGTTAC+TGG 0.264090 4_1:-86526395 Msa0581210:CDS
TGAGAGGGTGGAGGAGGTGG+AGG 0.275287 4_1:+86525744 None:intergenic
GCTTCATTAACTCCAAATAA+TGG 0.285010 4_1:-86526041 Msa0581210:CDS
GCTTAGTGGAGTGGAAAATA+AGG 0.302835 4_1:+86527423 None:intergenic
AAGAGTCAAGAAAATTCTTC+AGG 0.319616 4_1:-86527130 Msa0581210:CDS
TGAAACTGAAAATATTAATA+AGG 0.330846 4_1:-86526258 Msa0581210:CDS
ACAGAACAGAAATTGATTCT+TGG 0.330890 4_1:-86526418 Msa0581210:CDS
TAGTACATATGTGGTGGATA+TGG 0.331565 4_1:+86525897 None:intergenic
GTTAATTAGTGCTTAATGTA+AGG 0.337945 4_1:-86525600 Msa0581210:three_prime_UTR
TGTATAGCTTTGTCACATAA+TGG 0.351866 4_1:+86525662 None:intergenic
TGTTGATCAGCAGCTGGTTT+GGG 0.352102 4_1:+86527361 None:intergenic
ATTAGAAACACTCAAGTATC+AGG 0.357551 4_1:-86527326 Msa0581210:intron
CAGCCATTTGAAATGAAGTT+TGG 0.358581 4_1:+86527386 None:intergenic
ATCAAGAAGCTTTCAAGATC+AGG 0.368275 4_1:-86526350 Msa0581210:CDS
TTGTTGATCAGCAGCTGGTT+TGG 0.374527 4_1:+86527360 None:intergenic
GGATGAGAGGGTGGAGGAGG+TGG 0.374539 4_1:+86525741 None:intergenic
ATACTTGAGTGTTTCTAATC+CGG 0.381184 4_1:+86527330 None:intergenic
GCAGAGCATTACAATGCTAT+TGG 0.387344 4_1:+86525220 None:intergenic
AGCACTTCTTCTTATTATGT+TGG 0.397233 4_1:-86525807 Msa0581210:CDS
TCTAAGTGGGGTGTGTTTGT+TGG 0.404477 4_1:+86527461 None:intergenic
CAACCAAACTTCATTTCAAA+TGG 0.404986 4_1:-86527389 Msa0581210:exon
GTGTAGTTTGCTTGCTGGTA+TGG 0.419290 4_1:-86525339 Msa0581210:three_prime_UTR
TGCTTAATGTAAGGGAAAGT+GGG 0.420370 4_1:-86525591 Msa0581210:three_prime_UTR
GCTACTGCTGGAGGAGGGTG+TGG 0.422467 4_1:+86525873 None:intergenic
TAAGAAGAAGTGCTAGAAAC+AGG 0.428358 4_1:+86525816 None:intergenic
ATATGGTGTAGTTTGCTTGC+TGG 0.428624 4_1:-86525344 Msa0581210:three_prime_UTR
CTCACACCCTTTAAAAGCAT+TGG 0.430712 4_1:-86527489 Msa0581210:five_prime_UTR
TGATGACCATAGCTGTTGAT+TGG 0.433870 4_1:+86525720 None:intergenic
GTGCTTAATGTAAGGGAAAG+TGG 0.434606 4_1:-86525592 Msa0581210:three_prime_UTR
TTAGCATGCATGGGCATGAC+AGG 0.444727 4_1:+86525780 None:intergenic
GGTGGTGCTACTGCTGGAGG+AGG 0.445055 4_1:+86525867 None:intergenic
CCAGCAGTAGCACCACCCTA+TGG 0.447198 4_1:-86525861 Msa0581210:CDS
ATTGGATGAGAGGGTGGAGG+AGG 0.450211 4_1:+86525738 None:intergenic
GCATGCTGCTGAGGAAGTAC+TGG 0.452781 4_1:+86525927 None:intergenic
GATCACAAAGAAGGTCATAA+TGG 0.457322 4_1:-86526137 Msa0581210:CDS
TTAATTAGTGCTTAATGTAA+GGG 0.467475 4_1:-86525599 Msa0581210:three_prime_UTR
GGAGGCATAATAATTTCCTT+AGG 0.468365 4_1:+86525948 None:intergenic
AGTACATATGTGGTGGATAT+GGG 0.469995 4_1:+86525898 None:intergenic
ACGAAGAAAGCTTGTCTAAG+TGG 0.471666 4_1:+86527447 None:intergenic
AAAATTCTTCAGGGCATTGA+AGG 0.479304 4_1:-86527120 Msa0581210:intron
TTGGTACGTAGCTAGCTTAG+TGG 0.480383 4_1:+86527409 None:intergenic
TACTTGAGTGTTTCTAATCC+GGG 0.491731 4_1:+86527331 None:intergenic
CATAATAATTTCCTTAGGAA+TGG 0.491755 4_1:+86525953 None:intergenic
CTTAGTGGAGTGGAAAATAA+GGG 0.493762 4_1:+86527424 None:intergenic
GGAACTTGTTGATCAGCAGC+TGG 0.496704 4_1:+86527355 None:intergenic
TACTATACTATACATGTACA+TGG 0.497297 4_1:-86527516 Msa0581210:five_prime_UTR
CATTTGAAATGAAGTTTGGT+TGG 0.501265 4_1:+86527390 None:intergenic
AGTTTGCTTGCTGGTATGGT+GGG 0.503457 4_1:-86525335 Msa0581210:three_prime_UTR
CCATAGGGTGGTGCTACTGC+TGG 0.507340 4_1:+86525861 None:intergenic
TCTAGAGTAATTAGCATGCA+TGG 0.510452 4_1:+86525770 None:intergenic
AAATATTAATAAGGAGATCA+AGG 0.511134 4_1:-86526249 Msa0581210:CDS
CGAAGAAAGCTTGTCTAAGT+GGG 0.515764 4_1:+86527448 None:intergenic
TAGTTTGCTTGCTGGTATGG+TGG 0.517121 4_1:-86525336 Msa0581210:three_prime_UTR
CAGGGAAATCAGTAGAACTT+TGG 0.517951 4_1:-86526331 Msa0581210:CDS
GAAGATTCAGATCACAAAGA+AGG 0.518960 4_1:-86526146 Msa0581210:CDS
GAGAGGGTGGAGGAGGTGGA+GGG 0.528150 4_1:+86525745 None:intergenic
TAATGCAGATGTTGCAAAGT+CGG 0.537492 4_1:+86525433 None:intergenic
TCAAGAAGCTTTCAAGATCA+GGG 0.538607 4_1:-86526349 Msa0581210:CDS
TAGGGTGGTGCTACTGCTGG+AGG 0.544543 4_1:+86525864 None:intergenic
GTGGTGCTACTGCTGGAGGA+GGG 0.547041 4_1:+86525868 None:intergenic
CAGCAGTAGCACCACCCTAT+GGG 0.554674 4_1:-86525860 Msa0581210:CDS
TTGATTGGATGAGAGGGTGG+AGG 0.558090 4_1:+86525735 None:intergenic
GATAATAACAATAAAAGTGA+GGG 0.562957 4_1:-86526113 Msa0581210:CDS
TGTGTTATAGCTTAACCCAT+AGG 0.565506 4_1:+86525845 None:intergenic
GTGTTATAGCTTAACCCATA+GGG 0.565951 4_1:+86525846 None:intergenic
ATAGATGATGCAGGTGATGA+TGG 0.566616 4_1:-86526173 Msa0581210:CDS
AACATCTGCATTAGAAGTTG+CGG 0.568622 4_1:-86525423 Msa0581210:three_prime_UTR
AGAGTCAAGAAAATTCTTCA+GGG 0.568811 4_1:-86527129 Msa0581210:CDS
AAAAGTGAATGCCATTCCTA+AGG 0.569713 4_1:-86525964 Msa0581210:CDS
TTAACTCCAAATAATGGAGG+AGG 0.576698 4_1:-86526035 Msa0581210:CDS
AATTGATTCTTGGCAGCACA+AGG 0.578132 4_1:-86526408 Msa0581210:CDS
GTTGGTGAAAGTAGTCATGA+AGG 0.580432 4_1:-86526200 Msa0581210:CDS
CTAGAGTAATTAGCATGCAT+GGG 0.582346 4_1:+86525771 None:intergenic
GGGTTATAAGCATGCTGCTG+AGG 0.582921 4_1:+86525918 None:intergenic
GCTGATCAACAAGTTCCTCC+CGG 0.583544 4_1:-86527349 Msa0581210:CDS
GCTTAATGTAAGGGAAAGTG+GGG 0.583691 4_1:-86525590 Msa0581210:three_prime_UTR
TCTCATCCAATCAACAGCTA+TGG 0.586584 4_1:-86525726 Msa0581210:CDS
TAGCATGCATGGGCATGACA+GGG 0.586920 4_1:+86525781 None:intergenic
AATGATCATGAAGAATATGA+AGG 0.589711 4_1:-86525696 Msa0581210:CDS
TGATAATAACAATAAAAGTG+AGG 0.591536 4_1:-86526114 Msa0581210:CDS
GATCATGAAGAATATGAAGG+TGG 0.598780 4_1:-86525693 Msa0581210:CDS
CTGTTGATTGGATGAGAGGG+TGG 0.603779 4_1:+86525732 None:intergenic
AATAACAATAAAAGTGAGGG+TGG 0.610406 4_1:-86526110 Msa0581210:CDS
AAAAGACAATAATTGTGAGA+CGG 0.612655 4_1:-86527564 Msa0581210:five_prime_UTR
GGGTGTGGATAGTACATATG+TGG 0.614125 4_1:+86525888 None:intergenic
TGCTGCTGAGGAAGTACTGG+AGG 0.614651 4_1:+86525930 None:intergenic
AAAGTCTTGATCCACTGCGA+CGG 0.628481 4_1:-86527162 Msa0581210:CDS
AAGAACAGTACTGAACAAGT+TGG 0.630407 4_1:-86526218 Msa0581210:CDS
TGTGGATAGTACATATGTGG+TGG 0.633196 4_1:+86525891 None:intergenic
GAAGGTTGTATAGATGATGC+AGG 0.636887 4_1:-86526182 Msa0581210:CDS
ACGTAGCTAGCTTAGTGGAG+TGG 0.638739 4_1:+86527414 None:intergenic
CTTTACACATAAGATAGAGA+AGG 0.643549 4_1:+86525171 None:intergenic
ATAGCTGTTGATTGGATGAG+AGG 0.644634 4_1:+86525728 None:intergenic
AGCATGCATGGGCATGACAG+GGG 0.652187 4_1:+86525782 None:intergenic
TAGCTGTTGATTGGATGAGA+GGG 0.658334 4_1:+86525729 None:intergenic
ACTATACTATACATGTACAT+GGG 0.661840 4_1:-86527515 Msa0581210:five_prime_UTR
TCATTAACTCCAAATAATGG+AGG 0.678633 4_1:-86526038 Msa0581210:CDS
TTATAGCTTAACCCATAGGG+TGG 0.684519 4_1:+86525849 None:intergenic
GAAGAAAGCTTGTCTAAGTG+GGG 0.685656 4_1:+86527449 None:intergenic
TTGAGTGTTTCTAATCCGGG+AGG 0.702857 4_1:+86527334 None:intergenic
TGTGACAAAGCTATACAATG+TGG 0.703248 4_1:-86525656 Msa0581210:exon
GGTTACAGTTACTGGCAACG+TGG 0.704095 4_1:-86526387 Msa0581210:CDS
CTTAATGTAAGGGAAAGTGG+GGG 0.705125 4_1:-86525589 Msa0581210:three_prime_UTR
GACAAAGCTATACAATGTGG+AGG 0.713576 4_1:-86525653 Msa0581210:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAATAAATGCTATAT+TGG - chr4_1:86525164-86525183 Msa0581210:three_prime_UTR 10.0%
!! TGTAATAGAAAAAAAAAATA+TGG - chr4_1:86527433-86527452 Msa0581210:five_prime_UTR 10.0%
!!! TATTTTGAGAAAAAGAAAAA+AGG - chr4_1:86525204-86525223 Msa0581210:three_prime_UTR 15.0%
!! TAATAGCTTATTAAACATAT+TGG - chr4_1:86525965-86525984 Msa0581210:CDS 15.0%
!! TATATTTGATTAAAGTCTAA+CGG - chr4_1:86526315-86526334 Msa0581210:CDS 15.0%
!! AAAAAATCTTCAAAATCAAA+AGG - chr4_1:86526513-86526532 Msa0581210:intron 15.0%
!! TGAAACTGAAAATATTAATA+AGG - chr4_1:86526536-86526555 Msa0581210:intron 15.0%
!!! AAAAAACTATACTTTTTAAC+AGG + chr4_1:86527536-86527555 None:intergenic 15.0%
!!! TGTTAAAAAGTATAGTTTTT+TGG - chr4_1:86527535-86527554 Msa0581210:five_prime_UTR 15.0%
!!! GTTAAAAAGTATAGTTTTTT+GGG - chr4_1:86527536-86527555 Msa0581210:five_prime_UTR 15.0%
!!! TATATCATAGTTCTAAATTG+CGG + chr4_1:86525856-86525875 None:intergenic 20.0%
!! CTGATAAAATAAATACCATT+AGG + chr4_1:86525907-86525926 None:intergenic 20.0%
!! AAATATTAATAAGGAGATCA+AGG - chr4_1:86526545-86526564 Msa0581210:intron 20.0%
!! TGATAATAACAATAAAAGTG+AGG - chr4_1:86526680-86526699 Msa0581210:intron 20.0%
!! GATAATAACAATAAAAGTGA+GGG - chr4_1:86526681-86526700 Msa0581210:intron 20.0%
!! AAGAAAAAGAAAAACAAAAG+TGG - chr4_1:86526726-86526745 Msa0581210:intron 20.0%
!! TTAATTAGTGCTTAATGTAA+GGG - chr4_1:86527195-86527214 Msa0581210:intron 20.0%
!! AGATCTAGATTAAAAAAATG+TGG - chr4_1:86527395-86527414 Msa0581210:five_prime_UTR 20.0%
!! GATCTAGATTAAAAAAATGT+GGG - chr4_1:86527396-86527415 Msa0581210:five_prime_UTR 20.0%
!! ATCTAGATTAAAAAAATGTG+GGG - chr4_1:86527397-86527416 Msa0581210:five_prime_UTR 20.0%
!! ATACAAAGAAGAAGAAAAAA+AGG + chr4_1:86527510-86527529 None:intergenic 20.0%
! AAAAGACAATAATTGTGAGA+CGG - chr4_1:86525230-86525249 Msa0581210:three_prime_UTR 25.0%
! TACTATACTATACATGTACA+TGG - chr4_1:86525278-86525297 Msa0581210:three_prime_UTR 25.0%
! ACTATACTATACATGTACAT+GGG - chr4_1:86525279-86525298 Msa0581210:three_prime_UTR 25.0%
!! ATATTTTTCTGAGTACCTAA+TGG - chr4_1:86525889-86525908 Msa0581210:CDS 25.0%
! CATGTAATTAACTAAATTCC+AGG + chr4_1:86526038-86526057 None:intergenic 25.0%
!!! GATTTTTTATCTTTCTTCTC+AGG + chr4_1:86526501-86526520 None:intergenic 25.0%
! CATAATAATTTCCTTAGGAA+TGG + chr4_1:86526844-86526863 None:intergenic 25.0%
! AATGATCATGAAGAATATGA+AGG - chr4_1:86527098-86527117 Msa0581210:intron 25.0%
! GTTAATTAGTGCTTAATGTA+AGG - chr4_1:86527194-86527213 Msa0581210:intron 25.0%
!! AAATCGTCTTTTTACAAACT+AGG + chr4_1:86527273-86527292 None:intergenic 25.0%
!! TAGTTTGTAAAAAGACGATT+TGG - chr4_1:86527272-86527291 Msa0581210:intron 25.0%
!!! TTTTTGGGTTTGCTAAAAAA+AGG - chr4_1:86527551-86527570 Msa0581210:five_prime_UTR 25.0%
! GTTTTGCCAATGCTTTTAAA+GGG + chr4_1:86525314-86525333 None:intergenic 30.0%
! CATTTGAAATGAAGTTTGGT+TGG + chr4_1:86525407-86525426 None:intergenic 30.0%
CAACCAAACTTCATTTCAAA+TGG - chr4_1:86525405-86525424 Msa0581210:three_prime_UTR 30.0%
! ATACTTGAGTGTTTCTAATC+CGG + chr4_1:86525467-86525486 None:intergenic 30.0%
ATTAGAAACACTCAAGTATC+AGG - chr4_1:86525468-86525487 Msa0581210:three_prime_UTR 30.0%
AAGAGTCAAGAAAATTCTTC+AGG - chr4_1:86525664-86525683 Msa0581210:exon 30.0%
AGAGTCAAGAAAATTCTTCA+GGG - chr4_1:86525665-86525684 Msa0581210:exon 30.0%
AATGAAGACAAATACAACTG+AGG - chr4_1:86525770-86525789 Msa0581210:CDS 30.0%
CAATTATGGCTGCAATATAA+AGG + chr4_1:86525823-86525842 None:intergenic 30.0%
TTATATTGCAGCCATAATTG+CGG - chr4_1:86525823-86525842 Msa0581210:CDS 30.0%
TTAGAAGACTAACACTTAAG+TGG + chr4_1:86525948-86525967 None:intergenic 30.0%
ATTGACATGATCAATAAACC+TGG - chr4_1:86526017-86526036 Msa0581210:CDS 30.0%
TATATCCGTGACAATCATAA+CGG + chr4_1:86526347-86526366 None:intergenic 30.0%
TATGATTGTCACGGATATAT+AGG - chr4_1:86526348-86526367 Msa0581210:CDS 30.0%
ATGATTGTCACGGATATATA+GGG - chr4_1:86526349-86526368 Msa0581210:CDS 30.0%
! ACAGAACAGAAATTGATTCT+TGG - chr4_1:86526376-86526395 Msa0581210:CDS 30.0%
! TTTTTATCTTTCTTCTCAGG+AGG + chr4_1:86526498-86526517 None:intergenic 30.0%
AATAACAATAAAAGTGAGGG+TGG - chr4_1:86526684-86526703 Msa0581210:intron 30.0%
GCTTCATTAACTCCAAATAA+TGG - chr4_1:86526753-86526772 Msa0581210:intron 30.0%
TCATTAACTCCAAATAATGG+AGG - chr4_1:86526756-86526775 Msa0581210:intron 30.0%
!!! TTCACTTTTTCAAGTACTTG+TGG + chr4_1:86526819-86526838 None:intergenic 30.0%
AGCACTTCTTCTTATTATGT+TGG - chr4_1:86526987-86527006 Msa0581210:intron 30.0%
!! TGTATAGCTTTGTCACATAA+TGG + chr4_1:86527135-86527154 None:intergenic 30.0%
ATTAAAAAAATGTGGGGACA+AGG - chr4_1:86527403-86527422 Msa0581210:five_prime_UTR 30.0%
CTTTACACATAAGATAGAGA+AGG + chr4_1:86527626-86527645 None:intergenic 30.0%
! GGTTTTGCCAATGCTTTTAA+AGG + chr4_1:86525315-86525334 None:intergenic 35.0%
CTTAGTGGAGTGGAAAATAA+GGG + chr4_1:86525373-86525392 None:intergenic 35.0%
! CAGCCATTTGAAATGAAGTT+TGG + chr4_1:86525411-86525430 None:intergenic 35.0%
! TACTTGAGTGTTTCTAATCC+GGG + chr4_1:86525466-86525485 None:intergenic 35.0%
!! TTAGTAGACTTGTTAGAGCA+AGG + chr4_1:86525521-86525540 None:intergenic 35.0%
AGCAAAGAATAGATTCAGGA+GGG + chr4_1:86525549-86525568 None:intergenic 35.0%
GTTGAGCAAAGAATAGATTC+AGG + chr4_1:86525553-86525572 None:intergenic 35.0%
!! AAAATTCTTCAGGGCATTGA+AGG - chr4_1:86525674-86525693 Msa0581210:CDS 35.0%
AATAATACACACACACACCA+TGG - chr4_1:86525700-86525719 Msa0581210:CDS 35.0%
TTGGTTAAAGAGTAGTGTCT+TGG - chr4_1:86525984-86526003 Msa0581210:CDS 35.0%
ATCCACTTCAGACTTTATCA+TGG - chr4_1:86526111-86526130 Msa0581210:CDS 35.0%
ATCCATGATAAAGTCTGAAG+TGG + chr4_1:86526116-86526135 None:intergenic 35.0%
AATATACGTAAGAGTCGTAC+AGG + chr4_1:86526301-86526320 None:intergenic 35.0%
TGATTGTCACGGATATATAG+GGG - chr4_1:86526350-86526369 Msa0581210:CDS 35.0%
ATCAAGAAGCTTTCAAGATC+AGG - chr4_1:86526444-86526463 Msa0581210:intron 35.0%
TCAAGAAGCTTTCAAGATCA+GGG - chr4_1:86526445-86526464 Msa0581210:intron 35.0%
! AAGAACAGTACTGAACAAGT+TGG - chr4_1:86526576-86526595 Msa0581210:intron 35.0%
GAAGATTCAGATCACAAAGA+AGG - chr4_1:86526648-86526667 Msa0581210:intron 35.0%
GATCACAAAGAAGGTCATAA+TGG - chr4_1:86526657-86526676 Msa0581210:intron 35.0%
! TTAACTCCAAATAATGGAGG+AGG - chr4_1:86526759-86526778 Msa0581210:intron 35.0%
! TCTTTTCCTCCTCCATTATT+TGG + chr4_1:86526768-86526787 None:intergenic 35.0%
AAAAGTGAATGCCATTCCTA+AGG - chr4_1:86526830-86526849 Msa0581210:intron 35.0%
! GGAGGCATAATAATTTCCTT+AGG + chr4_1:86526849-86526868 None:intergenic 35.0%
!! AGTACATATGTGGTGGATAT+GGG + chr4_1:86526899-86526918 None:intergenic 35.0%
!! TAGTACATATGTGGTGGATA+TGG + chr4_1:86526900-86526919 None:intergenic 35.0%
GTGTTATAGCTTAACCCATA+GGG + chr4_1:86526951-86526970 None:intergenic 35.0%
TGTGTTATAGCTTAACCCAT+AGG + chr4_1:86526952-86526971 None:intergenic 35.0%
!! TAAGAAGAAGTGCTAGAAAC+AGG + chr4_1:86526981-86527000 None:intergenic 35.0%
CTAGAGTAATTAGCATGCAT+GGG + chr4_1:86527026-86527045 None:intergenic 35.0%
! TCTAGAGTAATTAGCATGCA+TGG + chr4_1:86527027-86527046 None:intergenic 35.0%
GATCATGAAGAATATGAAGG+TGG - chr4_1:86527101-86527120 Msa0581210:intron 35.0%
TGTGACAAAGCTATACAATG+TGG - chr4_1:86527138-86527157 Msa0581210:CDS 35.0%
TGCTTAATGTAAGGGAAAGT+GGG - chr4_1:86527203-86527222 Msa0581210:intron 35.0%
TAATGCAGATGTTGCAAAGT+CGG + chr4_1:86527364-86527383 None:intergenic 35.0%
AACATCTGCATTAGAAGTTG+CGG - chr4_1:86527371-86527390 Msa0581210:CDS 35.0%
!! CAATGCTTTTAAAGGGTGTG+AGG + chr4_1:86525307-86525326 None:intergenic 40.0%
! CTCACACCCTTTAAAAGCAT+TGG - chr4_1:86525305-86525324 Msa0581210:three_prime_UTR 40.0%
GAAGAAAGCTTGTCTAAGTG+GGG + chr4_1:86525348-86525367 None:intergenic 40.0%
CGAAGAAAGCTTGTCTAAGT+GGG + chr4_1:86525349-86525368 None:intergenic 40.0%
ACGAAGAAAGCTTGTCTAAG+TGG + chr4_1:86525350-86525369 None:intergenic 40.0%
GCTTAGTGGAGTGGAAAATA+AGG + chr4_1:86525374-86525393 None:intergenic 40.0%
GAGCAAAGAATAGATTCAGG+AGG + chr4_1:86525550-86525569 None:intergenic 40.0%
ATGTGATCATTCTACAGACG+TGG - chr4_1:86525603-86525622 Msa0581210:three_prime_UTR 40.0%
TGTGATCATTCTACAGACGT+GGG - chr4_1:86525604-86525623 Msa0581210:three_prime_UTR 40.0%
TCAAGTTTGCAACATAACCG+CGG + chr4_1:86525739-86525758 None:intergenic 40.0%
GAAGACAAATACAACTGAGG+CGG - chr4_1:86525773-86525792 Msa0581210:CDS 40.0%
GTTAAAGAGTAGTGTCTTGG+CGG - chr4_1:86525987-86526006 Msa0581210:CDS 40.0%
TTAAAGAGTAGTGTCTTGGC+GGG - chr4_1:86525988-86526007 Msa0581210:CDS 40.0%
TCCAACCGTTATGATTGTCA+CGG - chr4_1:86526339-86526358 Msa0581210:CDS 40.0%
! AATTGATTCTTGGCAGCACA+AGG - chr4_1:86526386-86526405 Msa0581210:CDS 40.0%
CAGGGAAATCAGTAGAACTT+TGG - chr4_1:86526463-86526482 Msa0581210:intron 40.0%
! GTTGGTGAAAGTAGTCATGA+AGG - chr4_1:86526594-86526613 Msa0581210:intron 40.0%
GAAGGTTGTATAGATGATGC+AGG - chr4_1:86526612-86526631 Msa0581210:intron 40.0%
ATAGATGATGCAGGTGATGA+TGG - chr4_1:86526621-86526640 Msa0581210:intron 40.0%
TGTGGATAGTACATATGTGG+TGG + chr4_1:86526906-86526925 None:intergenic 40.0%
TTATAGCTTAACCCATAGGG+TGG + chr4_1:86526948-86526967 None:intergenic 40.0%
! TAGCTGTTGATTGGATGAGA+GGG + chr4_1:86527068-86527087 None:intergenic 40.0%
! ATAGCTGTTGATTGGATGAG+AGG + chr4_1:86527069-86527088 None:intergenic 40.0%
TCTCATCCAATCAACAGCTA+TGG - chr4_1:86527068-86527087 Msa0581210:intron 40.0%
TGATGACCATAGCTGTTGAT+TGG + chr4_1:86527077-86527096 None:intergenic 40.0%
GACAAAGCTATACAATGTGG+AGG - chr4_1:86527141-86527160 Msa0581210:CDS 40.0%
GTGCTTAATGTAAGGGAAAG+TGG - chr4_1:86527202-86527221 Msa0581210:intron 40.0%
GCTTAATGTAAGGGAAAGTG+GGG - chr4_1:86527204-86527223 Msa0581210:intron 40.0%
CTTAATGTAAGGGAAAGTGG+GGG - chr4_1:86527205-86527224 Msa0581210:intron 40.0%
!! ATATGGTGTAGTTTGCTTGC+TGG - chr4_1:86527450-86527469 Msa0581210:five_prime_UTR 40.0%
! GCAGAGCATTACAATGCTAT+TGG + chr4_1:86527577-86527596 None:intergenic 40.0%
TCTAAGTGGGGTGTGTTTGT+TGG + chr4_1:86525336-86525355 None:intergenic 45.0%
! TTGGTACGTAGCTAGCTTAG+TGG + chr4_1:86525388-86525407 None:intergenic 45.0%
! TGTTGATCAGCAGCTGGTTT+GGG + chr4_1:86525436-86525455 None:intergenic 45.0%
TTGTTGATCAGCAGCTGGTT+TGG + chr4_1:86525437-86525456 None:intergenic 45.0%
! TTGAGTGTTTCTAATCCGGG+AGG + chr4_1:86525463-86525482 None:intergenic 45.0%
AAAGTCTTGATCCACTGCGA+CGG - chr4_1:86525632-86525651 Msa0581210:three_prime_UTR 45.0%
TCCGTGACAATCATAACGGT+TGG + chr4_1:86526343-86526362 None:intergenic 45.0%
CAGCACAAGGTTACAGTTAC+TGG - chr4_1:86526399-86526418 Msa0581210:CDS 45.0%
! TTCTTCTCAGGAGGCTTTTC+TGG + chr4_1:86526489-86526508 None:intergenic 45.0%
GGGTGTGGATAGTACATATG+TGG + chr4_1:86526909-86526928 None:intergenic 45.0%
!! GTGTAGTTTGCTTGCTGGTA+TGG - chr4_1:86527455-86527474 Msa0581210:five_prime_UTR 45.0%
!! TAGTTTGCTTGCTGGTATGG+TGG - chr4_1:86527458-86527477 Msa0581210:five_prime_UTR 45.0%
! AGTTTGCTTGCTGGTATGGT+GGG - chr4_1:86527459-86527478 Msa0581210:five_prime_UTR 45.0%
! ACGTAGCTAGCTTAGTGGAG+TGG + chr4_1:86525383-86525402 None:intergenic 50.0%
!! GGAACTTGTTGATCAGCAGC+TGG + chr4_1:86525442-86525461 None:intergenic 50.0%
GCTGATCAACAAGTTCCTCC+CGG - chr4_1:86525445-86525464 Msa0581210:three_prime_UTR 50.0%
!! ATGGTTCTGAATTGCGACCG+CGG - chr4_1:86525719-86525738 Msa0581210:CDS 50.0%
! GCGGACTACAACCGCAATTA+TGG + chr4_1:86525837-86525856 None:intergenic 50.0%
GGTTACAGTTACTGGCAACG+TGG - chr4_1:86526407-86526426 Msa0581210:CDS 50.0%
GGGTTATAAGCATGCTGCTG+AGG + chr4_1:86526879-86526898 None:intergenic 50.0%
TAGCATGCATGGGCATGACA+GGG + chr4_1:86527016-86527035 None:intergenic 50.0%
TTAGCATGCATGGGCATGAC+AGG + chr4_1:86527017-86527036 None:intergenic 50.0%
TTGATTGGATGAGAGGGTGG+AGG + chr4_1:86527062-86527081 None:intergenic 50.0%
! CTGTTGATTGGATGAGAGGG+TGG + chr4_1:86527065-86527084 None:intergenic 50.0%
! TTTTTGTGCAGCCGTCGCAG+TGG + chr4_1:86525646-86525665 None:intergenic 55.0%
GCGGTCGCAATTCAGAACCA+TGG + chr4_1:86525720-86525739 None:intergenic 55.0%
! AGTAGTGTCTTGGCGGGTCT+TGG - chr4_1:86525994-86526013 Msa0581210:CDS 55.0%
!! TGCTGCTGAGGAAGTACTGG+AGG + chr4_1:86526867-86526886 None:intergenic 55.0%
! GCATGCTGCTGAGGAAGTAC+TGG + chr4_1:86526870-86526889 None:intergenic 55.0%
CAGCAGTAGCACCACCCTAT+GGG - chr4_1:86526934-86526953 Msa0581210:intron 55.0%
AGCATGCATGGGCATGACAG+GGG + chr4_1:86527015-86527034 None:intergenic 55.0%
ATTGGATGAGAGGGTGGAGG+AGG + chr4_1:86527059-86527078 None:intergenic 55.0%
!! GTGGTGCTACTGCTGGAGGA+GGG + chr4_1:86526929-86526948 None:intergenic 60.0%
!! TAGGGTGGTGCTACTGCTGG+AGG + chr4_1:86526933-86526952 None:intergenic 60.0%
!! CCATAGGGTGGTGCTACTGC+TGG + chr4_1:86526936-86526955 None:intergenic 60.0%
CCAGCAGTAGCACCACCCTA+TGG - chr4_1:86526933-86526952 Msa0581210:intron 60.0%
GCTACTGCTGGAGGAGGGTG+TGG + chr4_1:86526924-86526943 None:intergenic 65.0%
!! GGTGGTGCTACTGCTGGAGG+AGG + chr4_1:86526930-86526949 None:intergenic 65.0%
GAGAGGGTGGAGGAGGTGGA+GGG + chr4_1:86527052-86527071 None:intergenic 65.0%
TGAGAGGGTGGAGGAGGTGG+AGG + chr4_1:86527053-86527072 None:intergenic 65.0%
GGATGAGAGGGTGGAGGAGG+TGG + chr4_1:86527056-86527075 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 86525161 86527655 86525161 ID=Msa0581210;Name=Msa0581210
chr4_1 mRNA 86525161 86527655 86525161 ID=Msa0581210-mRNA-1;Parent=Msa0581210;Name=Msa0581210-mRNA-1;_AED=0.12;_eAED=0.12;_QI=263|1|1|1|1|1|3|513|304
chr4_1 exon 86525161 86526446 86525161 ID=Msa0581210-mRNA-1:exon:32040;Parent=Msa0581210-mRNA-1
chr4_1 exon 86527121 86527196 86527121 ID=Msa0581210-mRNA-1:exon:32039;Parent=Msa0581210-mRNA-1
chr4_1 exon 86527327 86527655 86527327 ID=Msa0581210-mRNA-1:exon:32038;Parent=Msa0581210-mRNA-1
chr4_1 five_prime_UTR 86527393 86527655 86527393 ID=Msa0581210-mRNA-1:five_prime_utr;Parent=Msa0581210-mRNA-1
chr4_1 CDS 86527327 86527392 86527327 ID=Msa0581210-mRNA-1:cds;Parent=Msa0581210-mRNA-1
chr4_1 CDS 86527121 86527196 86527121 ID=Msa0581210-mRNA-1:cds;Parent=Msa0581210-mRNA-1
chr4_1 CDS 86525674 86526446 86525674 ID=Msa0581210-mRNA-1:cds;Parent=Msa0581210-mRNA-1
chr4_1 three_prime_UTR 86525161 86525673 86525161 ID=Msa0581210-mRNA-1:three_prime_utr;Parent=Msa0581210-mRNA-1
Gene Sequence

>Msa0581210

ATGGCTGCACCCAAACCAGCTGCTGATCAACAAGTTCCTCCCGGATTAGAAACACTCAAGTATCAGACGTGGGTTTTAAAAGTCTTGATCCACTGCGACGGCTGCACAAAAAGAGTCAAGAAAATTCTTCAGGGCATTGAAGGGGTTTACAGAACAGAAATTGATTCTTGGCAGCACAAGGTTACAGTTACTGGCAACGTGGATGCTGAGACTCTCATCAAGAAGCTTTCAAGATCAGGGAAATCAGTAGAACTTTGGCCAGAAAAGCCTCCTGAGAAGAAAGATAAAAAATCTTCAAAATCAAAAGGTGAAACTGAAAATATTAATAAGGAGATCAAGGAAGATCACAAGAACAGTACTGAACAAGTTGGTGAAAGTAGTCATGAAGGTTGTATAGATGATGCAGGTGATGATGGTGAAGAAGATTCAGATCACAAAGAAGGTCATAATGGTGATAATAACAATAAAAGTGAGGGTGGTGCTAAAAAGAAGAAGAAAAAGAAAAAGAAAAACAAAAGTGGTTCTGCTTCATTAACTCCAAATAATGGAGGAGGAAAAGAAATGATTAGTACAGAAACAATTGATTCACCACAAGTACTTGAAAAAGTGAATGCCATTCCTAAGGAAATTATTATGCCTCCAGTACTTCCTCAGCAGCATGCTTATAACCCATATCCACCACATATGTACTATCCACACCCTCCTCCAGCAGTAGCACCACCCTATGGGTTAAGCTATAACACATCATATCCTGTTTCTAGCACTTCTTCTTATTATGTTGGTGCCCCTGTCATGCCCATGCATGCTAATTACTCTAGATTCCCTCCACCTCCTCCACCCTCTCATCCAATCAACAGCTATGGTCATCATAATGATCATGAAGAATATGAAGGTGGATATTGCTCCATTATGTGA
Protein sequence

>Msa0581210

MAAPKPAADQQVPPGLETLKYQTWVLKVLIHCDGCTKRVKKILQGIEGVYRTEIDSWQHKVTVTGNVDAETLIKKLSRSGKSVELWPEKPPEKKDKKSSKSKGETENINKEIKEDHKNSTEQVGESSHEGCIDDAGDDGEEDSDHKEGHNGDNNNKSEGGAKKKKKKKKKNKSGSASLTPNNGGGKEMISTETIDSPQVLEKVNAIPKEIIMPPVLPQQHAYNPYPPHMYYPHPPPAVAPPYGLSYNTSYPVSSTSSYYVGAPVMPMHANYSRFPPPPPPSHPINSYGHHNDHEEYEGGYCSIM*