AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0603250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603250 MtrunA17_Chr4g0031411 95.252 337 12 2 1 336 1 334 0.0 585
Msa0603250 MtrunA17_Chr7g0237921 44.144 333 129 10 1 312 1 297 1.28e-61 200
Msa0603250 MtrunA17_Chr2g0328021 38.889 360 124 15 1 336 1 288 3.73e-57 187
Msa0603250 MtrunA17_Chr4g0010151 38.857 350 154 12 1 317 1 323 1.60e-55 185
Msa0603250 MtrunA17_Chr7g0223311 59.028 144 52 4 1 139 1 142 1.45e-46 161
Msa0603250 MtrunA17_Chr5g0411951 87.500 56 7 0 70 125 38 93 9.29e-33 124
Msa0603250 MtrunA17_Chr3g0117601 54.867 113 44 3 48 160 37 142 1.32e-32 124
Msa0603250 MtrunA17_Chr4g0047001 66.667 81 23 1 76 156 56 132 4.71e-32 123
Msa0603250 MtrunA17_Chr2g0326001 80.328 61 12 0 69 129 34 94 1.31e-31 120
Msa0603250 MtrunA17_Chr8g0374251 60.674 89 26 1 75 163 68 147 1.51e-31 121
Msa0603250 MtrunA17_Chr4g0030051 80.645 62 11 1 67 127 32 93 3.23e-31 119
Msa0603250 MtrunA17_Chr8g0367161 69.620 79 20 2 74 152 31 105 8.54e-31 119
Msa0603250 MtrunA17_Chr8g0346551 57.732 97 39 1 62 158 11 105 9.61e-31 117
Msa0603250 MtrunA17_Chr8g0374111 54.717 106 32 3 54 159 17 106 6.70e-30 114
Msa0603250 MtrunA17_Chr3g0127201 66.216 74 21 1 56 129 12 81 7.11e-30 115
Msa0603250 MtrunA17_Chr1g0188851 60.000 80 30 1 52 129 18 97 2.86e-29 114
Msa0603250 MtrunA17_Chr3g0126201 63.514 74 27 0 56 129 32 105 7.81e-29 113
Msa0603250 MtrunA17_Chr8g0341671 58.750 80 30 1 59 135 1 80 1.07e-28 110
Msa0603250 MtrunA17_Chr2g0282441 67.606 71 22 1 57 126 6 76 1.76e-28 112
Msa0603250 MtrunA17_Chr4g0061761 82.692 52 9 0 74 125 39 90 2.64e-28 112
Msa0603250 MtrunA17_Chr2g0282511 88.000 50 6 0 74 123 44 93 3.86e-28 111
Msa0603250 MtrunA17_Chr1g0177251 63.636 77 27 1 77 153 14 89 4.88e-28 110
Msa0603250 MtrunA17_Chr2g0308011 84.000 50 8 0 74 123 36 85 5.65e-27 105
Msa0603250 MtrunA17_Chr4g0047761 71.186 59 15 1 75 133 72 128 1.11e-24 102
Msa0603250 MtrunA17_Chr2g0282051 66.129 62 16 1 74 135 45 101 1.61e-24 100
Msa0603250 MtrunA17_Chr8g0355261 66.102 59 20 0 71 129 114 172 2.23e-24 102
Msa0603250 MtrunA17_Chr2g0283811 63.380 71 25 1 72 142 44 113 2.74e-24 97.1
Msa0603250 MtrunA17_Chr6g0460721 63.333 60 22 0 68 127 82 141 5.65e-24 100
Msa0603250 MtrunA17_Chr6g0453321 66.667 60 20 0 68 127 129 188 8.12e-24 101
Msa0603250 MtrunA17_Chr4g0042481 58.571 70 26 1 58 127 108 174 1.41e-23 100
Msa0603250 MtrunA17_Chr7g0217581 49.412 85 43 0 68 152 225 309 1.49e-23 101
Msa0603250 MtrunA17_Chr7g0254161 60.000 70 25 2 60 129 88 154 1.64e-23 100
Msa0603250 MtrunA17_Chr3g0092111 62.687 67 24 1 68 134 129 194 2.46e-23 100
Msa0603250 MtrunA17_Chr6g0460731 61.667 60 23 0 68 127 89 148 3.79e-23 98.6
Msa0603250 MtrunA17_Chr5g0417571 43.820 89 50 0 39 127 102 190 1.02e-21 94.0
Msa0603250 MtrunA17_Chr5g0417601 69.091 55 16 1 77 131 142 195 1.19e-20 91.7
Msa0603250 MtrunA17_Chr5g0417551 48.780 82 41 1 59 140 111 191 4.83e-20 89.7
Msa0603250 MtrunA17_Chr5g0417561 50.725 69 34 0 59 127 103 171 9.55e-20 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603250 AT3G55370.1 38.527 353 138 13 1 324 1 303 1.40e-51 174
Msa0603250 AT3G55370.2 42.164 268 104 8 1 251 1 234 2.41e-51 174
Msa0603250 AT3G55370.3 39.809 314 118 11 32 324 85 348 7.53e-49 168
Msa0603250 AT1G07640.1 42.308 286 145 10 57 335 3 275 1.39e-48 164
Msa0603250 AT1G07640.2 42.105 285 147 9 57 335 59 331 5.86e-48 164
Msa0603250 AT1G07640.3 42.105 285 147 9 57 335 67 339 9.70e-48 164
Msa0603250 AT2G37590.1 53.846 156 32 2 1 129 1 143 8.72e-47 161
Msa0603250 AT2G28810.1 36.685 368 160 15 1 332 1 331 1.21e-45 159
Msa0603250 AT5G02460.1 88.158 76 9 0 54 129 74 149 5.04e-44 156
Msa0603250 AT2G28810.2 38.776 294 125 11 57 332 58 314 2.14e-42 150
Msa0603250 AT5G60200.1 76.389 72 13 1 70 141 50 117 1.78e-34 127
Msa0603250 AT1G51700.1 58.889 90 29 1 70 159 28 109 1.18e-31 118
Msa0603250 AT5G60850.1 62.791 86 25 2 74 159 50 128 2.45e-31 120
Msa0603250 AT3G45610.1 53.409 88 39 1 70 155 35 122 2.99e-31 118
Msa0603250 AT4G38000.1 77.049 61 14 0 69 129 35 95 2.29e-30 116
Msa0603250 AT5G65590.1 81.034 58 11 0 75 132 41 98 4.77e-30 117
Msa0603250 AT3G21270.1 53.000 100 28 2 75 155 29 128 3.61e-29 111
Msa0603250 AT4G24060.1 50.000 112 56 0 69 180 47 158 1.92e-28 113
Msa0603250 AT1G28310.1 79.661 59 11 1 75 133 27 84 3.57e-28 111
Msa0603250 AT3G52440.1 70.588 68 16 1 77 144 27 90 4.20e-28 110
Msa0603250 AT3G52440.2 70.588 68 16 1 77 144 46 109 4.96e-28 110
Msa0603250 AT2G28510.1 73.846 65 13 1 74 138 47 107 5.38e-28 110
Msa0603250 AT1G28310.2 79.661 59 11 1 75 133 51 108 5.91e-28 111
Msa0603250 AT5G62940.1 77.193 57 13 0 69 125 67 123 7.54e-28 112
Msa0603250 AT3G61850.2 58.889 90 29 2 71 156 58 143 2.39e-27 108
Msa0603250 AT3G61850.3 58.889 90 29 2 71 156 58 143 2.39e-27 108
Msa0603250 AT5G66940.1 65.714 70 21 1 75 144 32 98 2.52e-27 107
Msa0603250 AT3G61850.1 58.889 90 29 2 71 156 70 155 2.83e-27 108
Msa0603250 AT3G61850.5 58.889 90 29 2 71 156 58 143 3.13e-27 109
Msa0603250 AT1G21340.1 71.875 64 12 1 74 137 37 94 3.35e-27 108
Msa0603250 AT3G61850.4 58.889 90 29 2 71 156 70 155 3.86e-27 109
Msa0603250 AT4G21050.1 47.581 124 50 3 68 190 17 126 4.61e-27 106
Msa0603250 AT3G50410.1 70.690 58 17 0 75 132 30 87 1.47e-26 106
Msa0603250 AT2G46590.1 56.250 80 35 0 43 122 36 115 2.86e-26 107
Msa0603250 AT2G46590.2 56.250 80 35 0 43 122 48 127 3.42e-26 107
Msa0603250 AT4G00940.1 63.514 74 23 2 59 129 50 122 1.17e-25 104
Msa0603250 AT4G00940.3 63.514 74 23 2 59 129 50 122 1.17e-25 104
Msa0603250 AT4G00940.2 63.514 74 23 2 59 129 50 122 1.17e-25 104
Msa0603250 AT1G64620.1 66.176 68 23 0 56 123 30 97 4.12e-25 104
Msa0603250 AT1G47655.1 70.000 60 16 1 66 123 18 77 2.00e-24 99.4
Msa0603250 AT2G34140.1 58.442 77 28 2 53 129 39 111 3.77e-24 97.4
Msa0603250 AT1G29160.1 69.492 59 17 1 71 129 58 115 1.24e-23 96.3
Msa0603250 AT4G21040.1 56.250 80 30 1 77 156 27 101 3.43e-23 96.7
Msa0603250 AT5G39660.3 66.667 63 20 1 67 129 130 191 3.52e-23 100
Msa0603250 AT5G39660.1 66.667 63 20 1 67 129 130 191 3.52e-23 100
Msa0603250 AT5G39660.2 66.667 63 20 1 67 129 130 191 3.52e-23 100
Msa0603250 AT1G26790.1 54.412 68 31 0 60 127 96 163 4.15e-23 99.0
Msa0603250 AT5G62430.1 56.000 75 31 2 58 131 36 109 4.38e-23 97.8
Msa0603250 AT3G47500.1 65.000 60 21 0 68 127 103 162 8.21e-23 99.0
Msa0603250 AT1G69570.1 61.667 60 23 0 68 127 125 184 2.08e-22 97.4
Msa0603250 AT4G21080.1 63.492 63 18 1 77 139 27 84 7.64e-22 93.2
Msa0603250 AT4G21030.1 54.098 61 25 1 68 128 17 74 1.86e-15 74.3

Find 113 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACTCATCTCTTATAAATT+TGG 0.183593 4_2:+47573183 Msa0603250:exon
GCAAGACATGTAGGCGTTAT+TGG 0.207030 4_2:+47572493 Msa0603250:CDS
AGTAATTTATGTACTATATA+TGG 0.208661 4_2:+47573365 Msa0603250:three_prime_UTR
GTTGTTGTTGATGTTGTTCT+TGG 0.217968 4_2:-47572785 None:intergenic
GATGAGAGAATGAGGGAATA+AGG 0.227115 4_2:+47571809 Msa0603250:five_prime_UTR
TCAAGAGCCACCGGTTAAAA+TGG 0.246428 4_2:+47573058 Msa0603250:CDS
TCTTGAGAGTTTGTTGAATT+AGG 0.247926 4_2:-47572603 None:intergenic
CCATTTGCTTGTTGATGATT+TGG 0.249048 4_2:-47572246 None:intergenic
ACCAACAACAATACTATTCT+TGG 0.250684 4_2:+47573138 Msa0603250:CDS
GTTTCTGCTTCTGCTGTTGC+TGG 0.257390 4_2:+47572870 Msa0603250:CDS
GATCATATGGGGTTTCAAAT+TGG 0.271163 4_2:+47572816 Msa0603250:CDS
TATTCTTGGAATGATTTATC+AGG 0.272738 4_2:+47573152 Msa0603250:CDS
TTTATAAAAGAAAACATAAA+AGG 0.276480 4_2:+47571973 Msa0603250:five_prime_UTR
TACTGGTGGCGGTGGTACTA+TGG 0.292815 4_2:+47572320 Msa0603250:CDS
TTTACCTGGTGGTGCCAATT+AGG 0.304320 4_2:-47572044 None:intergenic
CCTTCTCATCATGAACTAAT+TGG 0.327970 4_2:+47572654 Msa0603250:CDS
TTCATATCTTTGATCCATTC+CGG 0.333436 4_2:-47573206 None:intergenic
GTTATGTAGATCCTCCTAAT+TGG 0.350822 4_2:+47572030 Msa0603250:CDS
AACATTGAAGCTGCAGCATC+TGG 0.352153 4_2:+47572999 Msa0603250:CDS
TTCTCTTCTCTGTATTCCCT+TGG 0.355868 4_2:+47571912 Msa0603250:five_prime_UTR
AATTCCTAACCTACCCTTCA+TGG 0.365060 4_2:+47572695 Msa0603250:CDS
ACAACAACAACAAGATCATA+TGG 0.370266 4_2:+47572803 Msa0603250:CDS
ATGAGAGAATGAGGGAATAA+GGG 0.394979 4_2:+47571810 Msa0603250:five_prime_UTR
TCTGCTGTTGCTGGAGGTGT+TGG 0.398368 4_2:+47572879 Msa0603250:CDS
TTTCAAATTGGTATTGGTAA+TGG 0.399190 4_2:+47572828 Msa0603250:CDS
TCCAAGAATAGTATTGTTGT+TGG 0.406762 4_2:-47573139 None:intergenic
ATGGGGTTTCAAATTGGTAT+TGG 0.408013 4_2:+47572822 Msa0603250:CDS
AATACAGAGAAGAGAAAGAA+AGG 0.409902 4_2:-47571905 None:intergenic
GTAATTTATGTACTATATAT+GGG 0.411722 4_2:+47573366 Msa0603250:three_prime_UTR
CTACGACACCCTCCACCAAC+TGG 0.417773 4_2:-47572543 None:intergenic
GGACACTTTAGCGCTGGTTC+TGG 0.424523 4_2:-47572399 None:intergenic
TGAGAAGGGATTGCACTTGA+TGG 0.431346 4_2:-47572639 None:intergenic
CTGACGTTGTAATATCAATT+CGG 0.439770 4_2:+47573458 Msa0603250:three_prime_UTR
GCTCGATCAGCCATGGAGCC+TGG 0.444337 4_2:-47572354 None:intergenic
CCTCAAGTGGGAGGTAGTAC+TGG 0.448394 4_2:+47572303 Msa0603250:CDS
CCAAATCATCAACAAGCAAA+TGG 0.450966 4_2:+47572246 Msa0603250:CDS
TTGATGGCTCTTTAGGGAGA+TGG 0.453212 4_2:-47571947 None:intergenic
GCTGCAGCATCTGGATTTGT+TGG 0.454209 4_2:+47573008 Msa0603250:CDS
GATATGAAGTTTCGAACGAT+TGG 0.454492 4_2:+47573221 Msa0603250:three_prime_UTR
AAACAAAATCAATTACTAGA+AGG 0.455741 4_2:-47573319 None:intergenic
TCTTATAAATTTGGCCGGAA+TGG 0.459361 4_2:+47573192 Msa0603250:exon
GATTCAAAAGTATTCAAGAA+AGG 0.460724 4_2:-47572933 None:intergenic
CTTAGAAATGTTCCAGTTGG+TGG 0.472325 4_2:+47572531 Msa0603250:CDS
CAACAACAACAAGATCATAT+GGG 0.473282 4_2:+47572804 Msa0603250:CDS
CTCGATCAGACCAGGCTCCA+TGG 0.475078 4_2:+47572344 Msa0603250:CDS
GAGATATAGCTTCAAACTCT+AGG 0.480948 4_2:+47573030 Msa0603250:CDS
CCTCCCACTTGAGGTGGTAA+AGG 0.483237 4_2:-47572294 None:intergenic
TTTAGCGCTGGTTCTGGTGG+TGG 0.483860 4_2:-47572393 None:intergenic
ATACAGAGAAGAGAAAGAAA+GGG 0.484195 4_2:-47571904 None:intergenic
GGTAGGTTAGGAATTGTTTG+TGG 0.484498 4_2:-47572687 None:intergenic
AGCATTAAAGGAAGAGAGGA+GGG 0.487565 4_2:-47571875 None:intergenic
GAGGCCATGAAGGGTAGGTT+AGG 0.490552 4_2:-47572699 None:intergenic
AGAAATGTTCCAGTTGGTGG+AGG 0.497986 4_2:+47572534 Msa0603250:CDS
AAAGGAAGAGAGGAGGGAGG+AGG 0.506532 4_2:-47571869 None:intergenic
CCAATTAGTTCATGATGAGA+AGG 0.516486 4_2:-47572654 None:intergenic
AATTTATCAAGAACATCCCT+TGG 0.520144 4_2:+47573098 Msa0603250:CDS
CACTTTAGCGCTGGTTCTGG+TGG 0.522746 4_2:-47572396 None:intergenic
GAAGTAGCAAAATTTAGTGT+TGG 0.523027 4_2:-47572436 None:intergenic
TCCTCCTAATTGGCACCACC+AGG 0.528426 4_2:+47572040 Msa0603250:CDS
AAAGAGCATTAAAGGAAGAG+AGG 0.530419 4_2:-47571879 None:intergenic
ACTGGTGGCGGTGGTACTAT+GGG 0.530847 4_2:+47572321 Msa0603250:CDS
GGAAACGTTAACATGGGACT+TGG 0.531022 4_2:+47572750 Msa0603250:CDS
GAGAAGGGATTGCACTTGAT+GGG 0.532889 4_2:-47572638 None:intergenic
AGGAGGAAAGCAGCAATAAG+AGG 0.536872 4_2:-47571852 None:intergenic
TCATCTCTTATAAATTTGGC+CGG 0.539379 4_2:+47573187 Msa0603250:exon
CAACGAGGACACTTTAGCGC+TGG 0.540252 4_2:-47572405 None:intergenic
ACCTGGTGGTGCCAATTAGG+AGG 0.541280 4_2:-47572041 None:intergenic
TCTGCTTCTGCTGTTGCTGG+AGG 0.548891 4_2:+47572873 Msa0603250:CDS
GAGCATTAAAGGAAGAGAGG+AGG 0.549927 4_2:-47571876 None:intergenic
ATGGCTCTTTAGGGAGATGG+TGG 0.554378 4_2:-47571944 None:intergenic
AGATATAGCTTCAAACTCTA+GGG 0.556521 4_2:+47573031 Msa0603250:CDS
GGAGGTAGTACTGGTGGCGG+TGG 0.560481 4_2:+47572312 Msa0603250:CDS
ATTCTGCATCGAGGCCATGA+AGG 0.561082 4_2:-47572709 None:intergenic
AATCTGAGTCGTTATGTCGT+TGG 0.570962 4_2:+47572729 Msa0603250:CDS
CAATTAGTTCATGATGAGAA+GGG 0.573984 4_2:-47572653 None:intergenic
TGTCGTTGGAAACGTTAACA+TGG 0.575317 4_2:+47572743 Msa0603250:CDS
TCCACCTTTACCACCTCAAG+TGG 0.577272 4_2:+47572290 Msa0603250:CDS
ACTATGGGCTCGATCAGACC+AGG 0.580839 4_2:+47572336 Msa0603250:CDS
TTCTGCATCGAGGCCATGAA+GGG 0.581643 4_2:-47572708 None:intergenic
CCCACTTGAGGTGGTAAAGG+TGG 0.581750 4_2:-47572291 None:intergenic
ATTAAAGGAAGAGAGGAGGG+AGG 0.592350 4_2:-47571872 None:intergenic
GAAATGTTCCAGTTGGTGGA+GGG 0.594932 4_2:+47572535 Msa0603250:CDS
GCACTTAGAAATGTTCCAGT+TGG 0.600640 4_2:+47572528 Msa0603250:CDS
TATATTATTCAACTCAGTCA+TGG 0.602165 4_2:+47573269 Msa0603250:three_prime_UTR
AGAAGGGATTGCACTTGATG+GGG 0.604506 4_2:-47572637 None:intergenic
GTAGGCGTTATTGGACAAGA+GGG 0.605161 4_2:+47572502 Msa0603250:CDS
TAGGGCTAATCAAGAGCCAC+CGG 0.605644 4_2:+47573049 Msa0603250:CDS
GTCGTTGGAAACGTTAACAT+GGG 0.609381 4_2:+47572744 Msa0603250:CDS
GTCACTTCTGCAAGACATGT+AGG 0.611130 4_2:+47572484 Msa0603250:CDS
TGTAGGCGTTATTGGACAAG+AGG 0.616737 4_2:+47572501 Msa0603250:CDS
GCATCGAGGCCATGAAGGGT+AGG 0.623817 4_2:-47572704 None:intergenic
AATTGCTGCAAATTACGCCA+TGG 0.629104 4_2:-47572909 None:intergenic
TAAACGAGCTCGATCAGCCA+TGG 0.633447 4_2:-47572361 None:intergenic
CATGTCTTGCAGAAGTGACG+AGG 0.635723 4_2:-47572480 None:intergenic
GTGGGAGGTAGTACTGGTGG+CGG 0.638124 4_2:+47572309 Msa0603250:CDS
AACAACAACAAGATCATATG+GGG 0.646211 4_2:+47572805 Msa0603250:CDS
TATTATGTTCAGAGACACCA+AGG 0.647047 4_2:-47573115 None:intergenic
CCACCTTTACCACCTCAAGT+GGG 0.662217 4_2:+47572291 Msa0603250:CDS
CAAGTGGGAGGTAGTACTGG+TGG 0.664836 4_2:+47572306 Msa0603250:CDS
GTACTACCTCCCACTTGAGG+TGG 0.666584 4_2:-47572300 None:intergenic
TAGGCGTTATTGGACAAGAG+GGG 0.675252 4_2:+47572503 Msa0603250:CDS
AGGCGTTATTGGACAAGAGG+GGG 0.675862 4_2:+47572504 Msa0603250:CDS
ATGGTGGAGAATCAAACCAA+GGG 0.681127 4_2:-47571928 None:intergenic
CCAGTACTACCTCCCACTTG+AGG 0.681370 4_2:-47572303 None:intergenic
GATGGTGGAGAATCAAACCA+AGG 0.687483 4_2:-47571929 None:intergenic
CCTTTACCACCTCAAGTGGG+AGG 0.700568 4_2:+47572294 Msa0603250:CDS
GAGGTGTTGGAGTTGAACCA+TGG 0.701694 4_2:+47572892 Msa0603250:CDS
TGAGTTGAATAATATATGAG+TGG 0.703462 4_2:-47573262 None:intergenic
TTGAATAGAAGAGAAAACCA+TGG 0.707467 4_2:-47572006 None:intergenic
ATTATGTTCAGAGACACCAA+GGG 0.718424 4_2:-47573114 None:intergenic
AGAAGTGACGAGGTTGAGTG+AGG 0.724186 4_2:-47572470 None:intergenic
GTGTTGGTTGAATCGCAACG+AGG 0.733913 4_2:-47572420 None:intergenic
ACGACTCAGATTCTGCATCG+AGG 0.741695 4_2:-47572718 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATAAAAGAAAACATAAA+AGG + chr4_2:47571973-47571992 Msa0603250:five_prime_UTR 10.0%
!!! ATTTTAAAAAATTATTGTGA+TGG - chr4_2:47572183-47572202 None:intergenic 10.0%
!!! ATAAGCTTCTTTTTTTTAAT+GGG - chr4_2:47571734-47571753 None:intergenic 15.0%
!!! TATAAGCTTCTTTTTTTTAA+TGG - chr4_2:47571735-47571754 None:intergenic 15.0%
!! ATTAAAAAAAAGAAGCTTAT+AGG + chr4_2:47571734-47571753 Msa0603250:five_prime_UTR 15.0%
!!! TTTCTTTTATAAAGTTTTGA+TGG - chr4_2:47571966-47571985 None:intergenic 15.0%
!! ATGGAAAGAAATTAAAAAAA+GGG - chr4_2:47572207-47572226 None:intergenic 15.0%
!!! TGTTTATGTTTTTTATTTCA+TGG + chr4_2:47573337-47573356 Msa0603250:three_prime_UTR 15.0%
!! AGTAATTTATGTACTATATA+TGG + chr4_2:47573365-47573384 Msa0603250:three_prime_UTR 15.0%
!! GTAATTTATGTACTATATAT+GGG + chr4_2:47573366-47573385 Msa0603250:three_prime_UTR 15.0%
!!! ATATTTTTTAGGGTTTTTAG+TGG - chr4_2:47571774-47571793 None:intergenic 20.0%
!! ATGAAAATTAAAACTTTACC+TGG - chr4_2:47572061-47572080 None:intergenic 20.0%
!!! TTCACTTTCTTTTTTGAATT+TGG - chr4_2:47572090-47572109 None:intergenic 20.0%
!! GATGGAAAGAAATTAAAAAA+AGG - chr4_2:47572208-47572227 None:intergenic 20.0%
!!! AATAATTCATACACTTTTCA+TGG + chr4_2:47572969-47572988 Msa0603250:CDS 20.0%
!! AAACAAAATCAATTACTAGA+AGG - chr4_2:47573322-47573341 None:intergenic 20.0%
!! TAAAAGGATTTTCAATACCA+TGG + chr4_2:47571989-47572008 Msa0603250:exon 25.0%
! AAAATTAAAACTTTACCTGG+TGG - chr4_2:47572058-47572077 None:intergenic 25.0%
! ATATTCAAGAAGATGAAGTA+AGG - chr4_2:47572147-47572166 None:intergenic 25.0%
!! TTGAATTAGGAGATTTTGAA+CGG - chr4_2:47572593-47572612 None:intergenic 25.0%
!!! TTTCAAATTGGTATTGGTAA+TGG + chr4_2:47572828-47572847 Msa0603250:CDS 25.0%
!!! GATTCAAAAGTATTCAAGAA+AGG - chr4_2:47572936-47572955 None:intergenic 25.0%
! TATTCTTGGAATGATTTATC+AGG + chr4_2:47573152-47573171 Msa0603250:CDS 25.0%
! CTACTCATCTCTTATAAATT+TGG + chr4_2:47573183-47573202 Msa0603250:exon 25.0%
!! TGAGTTGAATAATATATGAG+TGG - chr4_2:47573265-47573284 None:intergenic 25.0%
! TATATTATTCAACTCAGTCA+TGG + chr4_2:47573269-47573288 Msa0603250:three_prime_UTR 25.0%
!!! GCTTCTTTTTTTTAATGGGT+TGG - chr4_2:47571730-47571749 None:intergenic 30.0%
!!! TTTTTTAGGGTTTTTAGTGG+AGG - chr4_2:47571771-47571790 None:intergenic 30.0%
AAAAAGAGATGAGAGAATGA+GGG + chr4_2:47571802-47571821 Msa0603250:five_prime_UTR 30.0%
!! AAAGGGAAAAAGAGCATTAA+AGG - chr4_2:47571890-47571909 None:intergenic 30.0%
ATACAGAGAAGAGAAAGAAA+GGG - chr4_2:47571907-47571926 None:intergenic 30.0%
AATACAGAGAAGAGAAAGAA+AGG - chr4_2:47571908-47571927 None:intergenic 30.0%
!! AAAGTTTTGATGGCTCTTTA+GGG - chr4_2:47571956-47571975 None:intergenic 30.0%
!! TAAAGTTTTGATGGCTCTTT+AGG - chr4_2:47571957-47571976 None:intergenic 30.0%
TTGAATAGAAGAGAAAACCA+TGG - chr4_2:47572009-47572028 None:intergenic 30.0%
! ATGGTTTTCTCTTCTATTCA+AGG + chr4_2:47572008-47572027 Msa0603250:CDS 30.0%
GAAGTAGCAAAATTTAGTGT+TGG - chr4_2:47572439-47572458 None:intergenic 30.0%
!! TCTTGAGAGTTTGTTGAATT+AGG - chr4_2:47572606-47572625 None:intergenic 30.0%
CAATTAGTTCATGATGAGAA+GGG - chr4_2:47572656-47572675 None:intergenic 30.0%
ACAACAACAACAAGATCATA+TGG + chr4_2:47572803-47572822 Msa0603250:CDS 30.0%
CAACAACAACAAGATCATAT+GGG + chr4_2:47572804-47572823 Msa0603250:CDS 30.0%
AACAACAACAAGATCATATG+GGG + chr4_2:47572805-47572824 Msa0603250:CDS 30.0%
AGATATAGCTTCAAACTCTA+GGG + chr4_2:47573031-47573050 Msa0603250:CDS 30.0%
!! CGATTTTCTTCCATTTTAAC+CGG - chr4_2:47573071-47573090 None:intergenic 30.0%
AATTTATCAAGAACATCCCT+TGG + chr4_2:47573098-47573117 Msa0603250:CDS 30.0%
ACCAACAACAATACTATTCT+TGG + chr4_2:47573138-47573157 Msa0603250:CDS 30.0%
TCCAAGAATAGTATTGTTGT+TGG - chr4_2:47573142-47573161 None:intergenic 30.0%
TCATCTCTTATAAATTTGGC+CGG + chr4_2:47573187-47573206 Msa0603250:exon 30.0%
TTCATATCTTTGATCCATTC+CGG - chr4_2:47573209-47573228 None:intergenic 30.0%
CTGACGTTGTAATATCAATT+CGG + chr4_2:47573458-47573477 Msa0603250:three_prime_UTR 30.0%
CAAAAAGAGATGAGAGAATG+AGG + chr4_2:47571801-47571820 Msa0603250:five_prime_UTR 35.0%
ATGAGAGAATGAGGGAATAA+GGG + chr4_2:47571810-47571829 Msa0603250:five_prime_UTR 35.0%
!! AAAGAGCATTAAAGGAAGAG+AGG - chr4_2:47571882-47571901 None:intergenic 35.0%
GTTATGTAGATCCTCCTAAT+TGG + chr4_2:47572030-47572049 Msa0603250:CDS 35.0%
TTGCTGCAGGTTAAAATAGA+TGG - chr4_2:47572226-47572245 None:intergenic 35.0%
!! CCATTTGCTTGTTGATGATT+TGG - chr4_2:47572249-47572268 None:intergenic 35.0%
CCAAATCATCAACAAGCAAA+TGG + chr4_2:47572246-47572265 Msa0603250:CDS 35.0%
CCAATTAGTTCATGATGAGA+AGG - chr4_2:47572657-47572676 None:intergenic 35.0%
CCTTCTCATCATGAACTAAT+TGG + chr4_2:47572654-47572673 Msa0603250:CDS 35.0%
! GTTGTTGTTGATGTTGTTCT+TGG - chr4_2:47572788-47572807 None:intergenic 35.0%
GATCATATGGGGTTTCAAAT+TGG + chr4_2:47572816-47572835 Msa0603250:CDS 35.0%
ATGGGGTTTCAAATTGGTAT+TGG + chr4_2:47572822-47572841 Msa0603250:CDS 35.0%
! ACTTTTGAATCAAACTCTGC+TGG + chr4_2:47572945-47572964 Msa0603250:CDS 35.0%
GAGATATAGCTTCAAACTCT+AGG + chr4_2:47573030-47573049 Msa0603250:CDS 35.0%
!! TTTTCTTCCATTTTAACCGG+TGG - chr4_2:47573068-47573087 None:intergenic 35.0%
ATTATGTTCAGAGACACCAA+GGG - chr4_2:47573117-47573136 None:intergenic 35.0%
TATTATGTTCAGAGACACCA+AGG - chr4_2:47573118-47573137 None:intergenic 35.0%
TCTTATAAATTTGGCCGGAA+TGG + chr4_2:47573192-47573211 Msa0603250:exon 35.0%
GATATGAAGTTTCGAACGAT+TGG + chr4_2:47573221-47573240 Msa0603250:three_prime_UTR 35.0%
GATGAGAGAATGAGGGAATA+AGG + chr4_2:47571809-47571828 Msa0603250:five_prime_UTR 40.0%
AGCATTAAAGGAAGAGAGGA+GGG - chr4_2:47571878-47571897 None:intergenic 40.0%
TTCTCTTCTCTGTATTCCCT+TGG + chr4_2:47571912-47571931 Msa0603250:five_prime_UTR 40.0%
! ATGGTGGAGAATCAAACCAA+GGG - chr4_2:47571931-47571950 None:intergenic 40.0%
GCACTTAGAAATGTTCCAGT+TGG + chr4_2:47572528-47572547 Msa0603250:CDS 40.0%
CTTAGAAATGTTCCAGTTGG+TGG + chr4_2:47572531-47572550 Msa0603250:CDS 40.0%
GGTAGGTTAGGAATTGTTTG+TGG - chr4_2:47572690-47572709 None:intergenic 40.0%
AATTCCTAACCTACCCTTCA+TGG + chr4_2:47572695-47572714 Msa0603250:CDS 40.0%
AATCTGAGTCGTTATGTCGT+TGG + chr4_2:47572729-47572748 Msa0603250:CDS 40.0%
TGTCGTTGGAAACGTTAACA+TGG + chr4_2:47572743-47572762 Msa0603250:CDS 40.0%
GTCGTTGGAAACGTTAACAT+GGG + chr4_2:47572744-47572763 Msa0603250:CDS 40.0%
AATTGCTGCAAATTACGCCA+TGG - chr4_2:47572912-47572931 None:intergenic 40.0%
AGGAGGAAAGCAGCAATAAG+AGG - chr4_2:47571855-47571874 None:intergenic 45.0%
ATTAAAGGAAGAGAGGAGGG+AGG - chr4_2:47571875-47571894 None:intergenic 45.0%
GAGCATTAAAGGAAGAGAGG+AGG - chr4_2:47571879-47571898 None:intergenic 45.0%
!! GATGGTGGAGAATCAAACCA+AGG - chr4_2:47571932-47571951 None:intergenic 45.0%
! TTGATGGCTCTTTAGGGAGA+TGG - chr4_2:47571950-47571969 None:intergenic 45.0%
! TTTACCTGGTGGTGCCAATT+AGG - chr4_2:47572047-47572066 None:intergenic 45.0%
!! GTTGATGATTTGGTTGCTGC+AGG - chr4_2:47572239-47572258 None:intergenic 45.0%
GTCACTTCTGCAAGACATGT+AGG + chr4_2:47572484-47572503 Msa0603250:CDS 45.0%
GCAAGACATGTAGGCGTTAT+TGG + chr4_2:47572493-47572512 Msa0603250:CDS 45.0%
TGTAGGCGTTATTGGACAAG+AGG + chr4_2:47572501-47572520 Msa0603250:CDS 45.0%
GTAGGCGTTATTGGACAAGA+GGG + chr4_2:47572502-47572521 Msa0603250:CDS 45.0%
TAGGCGTTATTGGACAAGAG+GGG + chr4_2:47572503-47572522 Msa0603250:CDS 45.0%
!! AGAAATGTTCCAGTTGGTGG+AGG + chr4_2:47572534-47572553 Msa0603250:CDS 45.0%
!! GAAATGTTCCAGTTGGTGGA+GGG + chr4_2:47572535-47572554 Msa0603250:CDS 45.0%
AGAAGGGATTGCACTTGATG+GGG - chr4_2:47572640-47572659 None:intergenic 45.0%
GAGAAGGGATTGCACTTGAT+GGG - chr4_2:47572641-47572660 None:intergenic 45.0%
TGAGAAGGGATTGCACTTGA+TGG - chr4_2:47572642-47572661 None:intergenic 45.0%
GGAAACGTTAACATGGGACT+TGG + chr4_2:47572750-47572769 Msa0603250:CDS 45.0%
AACATTGAAGCTGCAGCATC+TGG + chr4_2:47572999-47573018 Msa0603250:CDS 45.0%
! TCAAGAGCCACCGGTTAAAA+TGG + chr4_2:47573058-47573077 Msa0603250:CDS 45.0%
!!! TTGTTTTATTATATTTTTTA+GGG - chr4_2:47571784-47571803 None:intergenic 5.0%
!!! TTTGTTTTATTATATTTTTT+AGG - chr4_2:47571785-47571804 None:intergenic 5.0%
! ATGGCTCTTTAGGGAGATGG+TGG - chr4_2:47571947-47571966 None:intergenic 50.0%
TCCACCTTTACCACCTCAAG+TGG + chr4_2:47572290-47572309 Msa0603250:CDS 50.0%
CCACCTTTACCACCTCAAGT+GGG + chr4_2:47572291-47572310 Msa0603250:CDS 50.0%
TAAACGAGCTCGATCAGCCA+TGG - chr4_2:47572364-47572383 None:intergenic 50.0%
!! GTGTTGGTTGAATCGCAACG+AGG - chr4_2:47572423-47572442 None:intergenic 50.0%
AGAAGTGACGAGGTTGAGTG+AGG - chr4_2:47572473-47572492 None:intergenic 50.0%
CATGTCTTGCAGAAGTGACG+AGG - chr4_2:47572483-47572502 None:intergenic 50.0%
AGGCGTTATTGGACAAGAGG+GGG + chr4_2:47572504-47572523 Msa0603250:CDS 50.0%
TTCTGCATCGAGGCCATGAA+GGG - chr4_2:47572711-47572730 None:intergenic 50.0%
ATTCTGCATCGAGGCCATGA+AGG - chr4_2:47572712-47572731 None:intergenic 50.0%
ACGACTCAGATTCTGCATCG+AGG - chr4_2:47572721-47572740 None:intergenic 50.0%
GTTTCTGCTTCTGCTGTTGC+TGG + chr4_2:47572870-47572889 Msa0603250:CDS 50.0%
!! GAGGTGTTGGAGTTGAACCA+TGG + chr4_2:47572892-47572911 Msa0603250:CDS 50.0%
GCTGCAGCATCTGGATTTGT+TGG + chr4_2:47573008-47573027 Msa0603250:CDS 50.0%
TAGGGCTAATCAAGAGCCAC+CGG + chr4_2:47573049-47573068 Msa0603250:CDS 50.0%
AAAGGAAGAGAGGAGGGAGG+AGG - chr4_2:47571872-47571891 None:intergenic 55.0%
TCCTCCTAATTGGCACCACC+AGG + chr4_2:47572040-47572059 Msa0603250:CDS 55.0%
! ACCTGGTGGTGCCAATTAGG+AGG - chr4_2:47572044-47572063 None:intergenic 55.0%
CCCACTTGAGGTGGTAAAGG+TGG - chr4_2:47572294-47572313 None:intergenic 55.0%
CCTCCCACTTGAGGTGGTAA+AGG - chr4_2:47572297-47572316 None:intergenic 55.0%
CCTTTACCACCTCAAGTGGG+AGG + chr4_2:47572294-47572313 Msa0603250:CDS 55.0%
GTACTACCTCCCACTTGAGG+TGG - chr4_2:47572303-47572322 None:intergenic 55.0%
CCAGTACTACCTCCCACTTG+AGG - chr4_2:47572306-47572325 None:intergenic 55.0%
CCTCAAGTGGGAGGTAGTAC+TGG + chr4_2:47572303-47572322 Msa0603250:CDS 55.0%
! CAAGTGGGAGGTAGTACTGG+TGG + chr4_2:47572306-47572325 Msa0603250:CDS 55.0%
!! TACTGGTGGCGGTGGTACTA+TGG + chr4_2:47572320-47572339 Msa0603250:CDS 55.0%
!! ACTGGTGGCGGTGGTACTAT+GGG + chr4_2:47572321-47572340 Msa0603250:CDS 55.0%
ACTATGGGCTCGATCAGACC+AGG + chr4_2:47572336-47572355 Msa0603250:CDS 55.0%
!! TTTAGCGCTGGTTCTGGTGG+TGG - chr4_2:47572396-47572415 None:intergenic 55.0%
!! CACTTTAGCGCTGGTTCTGG+TGG - chr4_2:47572399-47572418 None:intergenic 55.0%
!! GGACACTTTAGCGCTGGTTC+TGG - chr4_2:47572402-47572421 None:intergenic 55.0%
CAACGAGGACACTTTAGCGC+TGG - chr4_2:47572408-47572427 None:intergenic 55.0%
GAGGCCATGAAGGGTAGGTT+AGG - chr4_2:47572702-47572721 None:intergenic 55.0%
TCTGCTTCTGCTGTTGCTGG+AGG + chr4_2:47572873-47572892 Msa0603250:CDS 55.0%
! TCTGCTGTTGCTGGAGGTGT+TGG + chr4_2:47572879-47572898 Msa0603250:CDS 55.0%
!! GTGGGAGGTAGTACTGGTGG+CGG + chr4_2:47572309-47572328 Msa0603250:CDS 60.0%
CTCGATCAGACCAGGCTCCA+TGG + chr4_2:47572344-47572363 Msa0603250:CDS 60.0%
CTACGACACCCTCCACCAAC+TGG - chr4_2:47572546-47572565 None:intergenic 60.0%
GCATCGAGGCCATGAAGGGT+AGG - chr4_2:47572707-47572726 None:intergenic 60.0%
!! GGAGGTAGTACTGGTGGCGG+TGG + chr4_2:47572312-47572331 Msa0603250:CDS 65.0%
GCTCGATCAGCCATGGAGCC+TGG - chr4_2:47572357-47572376 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 47571716 47573493 47571716 ID=Msa0603250;Name=Msa0603250
chr4_2 mRNA 47571716 47573493 47571716 ID=Msa0603250-mRNA-1;Parent=Msa0603250;Name=Msa0603250-mRNA-1;_AED=0.01;_eAED=0.01;_QI=292|1|1|1|1|1|2|294|336
chr4_2 exon 47571716 47572061 47571716 ID=Msa0603250-mRNA-1:exon:10891;Parent=Msa0603250-mRNA-1
chr4_2 exon 47572243 47573493 47572243 ID=Msa0603250-mRNA-1:exon:10892;Parent=Msa0603250-mRNA-1
chr4_2 five_prime_UTR 47571716 47572007 47571716 ID=Msa0603250-mRNA-1:five_prime_utr;Parent=Msa0603250-mRNA-1
chr4_2 CDS 47572008 47572061 47572008 ID=Msa0603250-mRNA-1:cds;Parent=Msa0603250-mRNA-1
chr4_2 CDS 47572243 47573199 47572243 ID=Msa0603250-mRNA-1:cds;Parent=Msa0603250-mRNA-1
chr4_2 three_prime_UTR 47573200 47573493 47573200 ID=Msa0603250-mRNA-1:three_prime_utr;Parent=Msa0603250-mRNA-1
Gene Sequence

>Msa0603250

ATGGTTTTCTCTTCTATTCAAGGTTATGTAGATCCTCCTAATTGGCACCACCAGCAACCAAATCATCAACAAGCAAATGGAAGCAATAACACTCAACTTCTTCCACCTTTACCACCTCAAGTGGGAGGTAGTACTGGTGGCGGTGGTACTATGGGCTCGATCAGACCAGGCTCCATGGCTGATCGAGCTCGTTTAGCAAAACTACCACCACCAGAACCAGCGCTAAAGTGTCCTCGTTGCGATTCAACCAACACTAAATTTTGCTACTTCAACAACTACAGCCTCACTCAACCTCGTCACTTCTGCAAGACATGTAGGCGTTATTGGACAAGAGGGGGTGCACTTAGAAATGTTCCAGTTGGTGGAGGGTGTCGTAGAAACAACAAGAAAAACAAAAGAAGCCGTTCAAAATCTCCTAATTCAACAAACTCTCAAGAAAAACAAACTTCCCCATCAAGTGCAATCCCTTCTCATCATGAACTAATTGGTCATCAGTTACCACAAACAATTCCTAACCTACCCTTCATGGCCTCGATGCAGAATCTGAGTCGTTATGTCGTTGGAAACGTTAACATGGGACTTGGTTTGCGTGAGATCCAAGAACAACATCAACAACAACAACAACAAGATCATATGGGGTTTCAAATTGGTATTGGTAATGGTAACTCAACTTCTGTTTCTGTTTCTGCTTCTGCTGTTGCTGGAGGTGTTGGAGTTGAACCATGGCGTAATTTGCAGCAATTTCCTTTCTTGAATACTTTTGAATCAAACTCTGCTGGAAATAATTCATACACTTTTCATGGAGAAAGTAACATTGAAGCTGCAGCATCTGGATTTGTTGGAGATATAGCTTCAAACTCTAGGGCTAATCAAGAGCCACCGGTTAAAATGGAAGAAAATCGAGCTTTAAATTTATCAAGAACATCCCTTGGTGTCTCTGAACATAATAACCAACAACAATACTATTCTTGGAATGATTTATCAGGCTCTTCAACTACTCATCTCTTATAA
Protein sequence

>Msa0603250

MVFSSIQGYVDPPNWHHQQPNHQQANGSNNTQLLPPLPPQVGGSTGGGGTMGSIRPGSMADRARLAKLPPPEPALKCPRCDSTNTKFCYFNNYSLTQPRHFCKTCRRYWTRGGALRNVPVGGGCRRNNKKNKRSRSKSPNSTNSQEKQTSPSSAIPSHHELIGHQLPQTIPNLPFMASMQNLSRYVVGNVNMGLGLREIQEQHQQQQQQDHMGFQIGIGNGNSTSVSVSASAVAGGVGVEPWRNLQQFPFLNTFESNSAGNNSYTFHGESNIEAAASGFVGDIASNSRANQEPPVKMEENRALNLSRTSLGVSEHNNQQQYYSWNDLSGSSTTHLL*